CN104054498B - A kind of cuttage and seedling culture method of tangut dragonhead - Google Patents

A kind of cuttage and seedling culture method of tangut dragonhead Download PDF

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CN104054498B
CN104054498B CN201410329469.XA CN201410329469A CN104054498B CN 104054498 B CN104054498 B CN 104054498B CN 201410329469 A CN201410329469 A CN 201410329469A CN 104054498 B CN104054498 B CN 104054498B
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cuttage
tangut dragonhead
root
cutting
dragonhead
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CN104054498A (en
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泽仁旺姆
尼珍
郭尚磊
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TIBETAN AUTONOMOUS REGION PLATEAU BIOLOGY INSTITUTE
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TIBETAN AUTONOMOUS REGION PLATEAU BIOLOGY INSTITUTE
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Abstract

The present invention relates to technical field of plant asexual propagation, especially relate to a kind of cuttage and seedling culture method of tangut dragonhead.Because tangut dragonhead just needs when in traditional Tibetan medicine is used as medicine, plant does not bloom to pluck, therefore cannot collect seed, utilizing cuttage to solve tangut dragonhead breeding problem is an effective approach.The present invention works out a kind of effective cuttage and seedling culture method, makes the rooting rate of tangut dragonhead cuttage reach 60%.

Description

A kind of cuttage and seedling culture method of tangut dragonhead
Technical field
The invention belongs to technical field of plant asexual propagation, especially relate to a kind of cuttage and seedling culture method of tangut dragonhead.
Technical background
Tangut dragonhead (DracocephalumtanguticumMaxim) belongs to the blue or green Cymbidium herbaceos perennial of Labiatae, has taste.Primary growth on the riverbank in dry river valley, field, careless beach or pine forest edge, height above sea level 3000-4600 rice.Produce most of county of Qamdo Prefecture, part county and the ground such as Lhasa, Linzhou such as Suo County, Bange of Nagqu Diqu.All herbal medicine, controls gastritis, hepatitis, dizziness, tired, the arthritis of god and scabies.Its distributed areas are comparatively wide, but grow sparse, and output is lower.Tangut dragonhead, except medicinal, is popular as traditional aromatic plant among the people, and therefore, excavate phenomenon seriously, resource amount reduces year by year, and reserve is less.
Because tangut dragonhead just needs when in traditional Tibetan medicine is used as medicine, plant does not bloom to pluck, therefore cannot collect seed, utilizing cuttage to solve tangut dragonhead breeding problem is an effective approach.
Tangut dragonhead is one of Tibetan medicine traditional medicine, but document mainly describes the aspect situation such as chemical composition and pharmacological research of tangut dragonhead at home and abroad, almost do not relate to the research to tangut dragonhead seedling growing process aspect, the research of the present invention to a kind of tangut dragonhead cuttage technique more belongs to the first.
Cottage propagation is the important means that clone is bred, and the excellent hereditary feature of parent can be inherited with chapter and verse, and nursery stock production is healthy and strong fast.Therefore, carry out the research of tangut dragonhead cutting propagation, its fine-variety breeding and Popularization And Development tool are of great significance.
Summary of the invention
The object of the present invention is to provide a kind of cuttage raise seedling technique method of tangut dragonhead, being intended to solve tangut dragonhead just needs when in traditional Tibetan medicine is used as medicine, plant does not bloom to pluck, and therefore cannot collect seed to carry out the problem of seeding and seedling raising.
The present invention is achieved in that a kind of cuttage raise seedling technique method of tangut dragonhead, it is characterized in that, described method comprises the steps:
The selection of step one, material: select that First Year is newly sent out, the tender stem of not yet hollow; And cutting grows 5 ~ 8cm, with two panels young leaves;
Step 2, by above-mentioned cutting equably cuttage inside slotting machine; Cuttage seeding row spacing 5cm × 5cm; Insertion depth is 1/3 ~ 1/2 of cutting length, compacting spigot after inserting; Described slotting machine is the long 40cm of thin river sand (granularity of thin river sand was 40 mesh sieves), the hollow out bamboo basket of wide 30cm that are paved with degree of depth 5cm, and this bamboo basket is positioned over shady and cool, ventilation location in greenhouse;
Water permeable with thin eye watering can after step 3, cuttage, depending on seedbed and the timely trickle of cuttings humidity condition, humidity remains on more than 85%;
Step 4, insert after management: cuttage 30d " Invest, Then Investigate " cuttage root-taking situation, to rooting rate, take root number and root length carry out observational record statistics.
About the selection of material, because tangut dragonhead is herbaceos perennial, along with the stem of growth tangut dragonhead can hollow gradually, thus need to select First Year newly to send out, the tender stem of not yet hollow; And cutting being grown 5 ~ 8cm, being tried material with two panels young leaves as supplying.
Cuttage has fast, the easy collection of reproduction speed, little, the convenient management of inheritance change, and some are had to the plant of merit, seminal propagation can make the character variation of plant, and cuttage then can make the offspring of breeding still have former merit.
About the effect that cottage method of the present invention has, one of technological difficulties due to this cottage method are exactly the selection of cutting material, because tangut dragonhead is herbaceos perennial, along with the stem of growth tangut dragonhead can hollow gradually, thus need to select First Year newly to send out, the tender stem of not yet hollow; Select ventilative good thin river sand can ensure to obtain good cutting experiment as cutting medium simultaneously.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Be selected in this Cutting test and carry out under intelligence temperature-controlling chamber of Lhasa district environment, carry out cuttage on April 8th, 2013.Cuttage Material selec-tion is collected in the strain in field.First two groups of cuttage comparative trials are set: 1. select First Year newly send out, not yet the tender stem of hollow be cutting, adopt different cutting medium: thin river sand and rural area soil, cuttage 30 days rooting rate in thin river sand matrix is 60%, and rural area soil is the rooting rate 14.8% of matrix.2. select thin river sand to be matrix, adopt different cutting: First Year is newly sent out, the not yet tender stem of hollow and the branch stem of hollow, and cuttage 30 days, the branch stem-root rate of hollow is 14.6%.
The selection of step one, material: select that First Year is newly sent out, the tender stem of not yet hollow; And cutting grows 5 ~ 8cm, with two panels young leaves;
Step 2, by above-mentioned cutting equably cuttage inside slotting machine; Cuttage seeding row spacing 5cm × 5cm; Insertion depth is 1/3 ~ 1/2 of cutting length, compacting spigot after inserting; Described slotting machine is the long 40cm of the thin river sand being paved with degree of depth 5cm, the hollow out bamboo basket of wide 30cm, and this bamboo basket is positioned over shady and cool, ventilation location in greenhouse;
Water permeable with thin eye watering can after step 3, cuttage, depending on seedbed and the timely trickle of cuttings humidity condition, humidity remains on more than 85%;
Step 4, slotting rear management: cuttage 30d " Invest, Then Investigate " cuttage root-taking situation, carry out observational record statistics to rooting rate, number of taking root, root length.
Carry out tangut dragonhead Cutting test according to the method described above, to rooting rate and root progress row observation statistics after 30d, result is as shown in table 1.
Table 1 tangut dragonhead cuttage root-taking situation
Cuttage strain number To take root strain number Rooting rate The longest root One-level root is on average long On average to take root number
28 17 60% 5.6cm 2.5cm 9.7 root
As can be seen from Table 1, it is feasible when tangut dragonhead carries out cottage propagation.The rooting rate of cuttage reaches more than 60%, and number of on average taking root also reaches 9.7, the long 2.50cm of average primary root.
After 30d, the situation of taking root of the tangut dragonhead cutting that every strain is taken root is added up, have recorded in detail every strain take root number and root long, result is as shown in table 2.
Table 2 tangut dragonhead rooting of cuttings situation
Numbering 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
To take root number 4 9 4 13 11 9 11 6 11 15 12 19 11 6 4 7 13
Average root is long 0.95 3.08 2.03 3.90 2.72 2.26 3.15 1.65 2.40 2.61 2.19 2.40 2.23 1.35 2.15 1.20 3.15
As can be seen from Table 2, tangut dragonhead rooting of cuttings number is minimum is 4, reaches at most 19, and at 0.95cm ~ 3.9cm not etc., it is feasible for utilizing the technology of cuttage to carry out breeding as can be seen here for the average root length of each cutting.
The cuttage and seedling culture method of a kind of tangut dragonhead of the present invention is described by concrete example, those skilled in the art can use for reference content of the present invention, the links such as suitable feed change, process conditions realize other object corresponding, its relevant change does not all depart from content of the present invention, all similar replacements and change will become apparent to those skilled in the art that and be all deemed to be included within scope of the present invention.

Claims (1)

1. a cuttage raise seedling technique method for tangut dragonhead, is characterized in that, described method comprises the steps:
The selection of step one, material: select that First Year is newly sent out, the tender stem of not yet hollow; And cutting grows 5 ~ 8cm, with two panels young leaves;
Step 2, by above-mentioned cutting equably cuttage inside slotting machine; Cuttage seeding row spacing 5cm × 5cm; Insertion depth is 1/3 ~ 1/2 of cutting length, compacting spigot after inserting; Described slotting machine is the long 40cm of the thin river sand being paved with degree of depth 5cm, the hollow out bamboo basket of wide 30cm, and the granularity of described thin river sand was 40 mesh sieves; This bamboo basket is positioned over shady and cool, ventilation location in greenhouse;
Water permeable with thin eye watering can after step 3, cuttage, depending on seedbed and the timely trickle of cuttings humidity condition, humidity remains on more than 85%;
Step 4, insert after management: cuttage 30d " Invest, Then Investigate " cuttage root-taking situation, to rooting rate, take root number and root length carry out observational record statistics.
CN201410329469.XA 2014-07-11 2014-07-11 A kind of cuttage and seedling culture method of tangut dragonhead Active CN104054498B (en)

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CN105309314B (en) * 2015-11-23 2017-08-25 内蒙古科技大学 A kind of method that anaesthetic Dracocephalum moldavica Tissue Culture Regeneration System is set up
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