CN103998658B - 用于标记dna-编码文库的方法 - Google Patents

用于标记dna-编码文库的方法 Download PDF

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CN103998658B
CN103998658B CN201280053930.2A CN201280053930A CN103998658B CN 103998658 B CN103998658 B CN 103998658B CN 201280053930 A CN201280053930 A CN 201280053930A CN 103998658 B CN103998658 B CN 103998658B
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nucleotides
headpiece
tag
building block
ligation
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CN103998658A (zh
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安东尼·D·基夫
理查德·W·瓦格纳
亚历山大·利托夫奇克
马修·克拉克
约翰·W·科佐
保罗·A·森特雷拉
克里斯托弗·D·赫普
张颖
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X Chem Inc
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/16—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support involving encoding steps
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support

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CN201280053930.2A 2011-09-07 2012-09-07 用于标记dna-编码文库的方法 Expired - Fee Related CN103998658B (zh)

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US201161531820P 2011-09-07 2011-09-07
US61/531,820 2011-09-07
US201161536929P 2011-09-20 2011-09-20
US61/536,929 2011-09-20
PCT/US2012/054228 WO2013036810A1 (en) 2011-09-07 2012-09-07 Methods for tagging dna-encoded libraries

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CN103998658B true CN103998658B (zh) 2021-06-18

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