CN103976110A - Snow chrysanthemum tea Jane and preparation method thereof - Google Patents
Snow chrysanthemum tea Jane and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a snow chrysanthemum tea Jane and a preparation method thereof. The preparation method comprises the steps of adding water into snow chrysanthemum powder according to the weight ratio of 1:30, carrying out ultrasonic treatment at the temperature of 55 DEG C and the power of 160w for 15min after thermostatic waterbath, carrying out enzymatic hydrolysis, adding 1% of cellulose, 1% of pectinase and 1.5% of papain, carrying out enzymatic hydrolysis at the temperature of 55 DEG C for 3h, deactivating at the temperature of 100 DEG C for 5min, centrifuging at the rotating speed of 3500r/min for 15min to obtain a supernatant, adding beta-cyclodextrin and maltodextrin, and carrying out spray-drying to obtain a finished product, wherein the mass of a beta-cyclodextrin and maltodextrin mixture to the mass of a solid matter of the snow chrysanthemum powder is 2:3. The prepared snow chrysanthemum tea Jane has the effects of reducing blood glucose and fat, has a therapeutic effect for diabetic patients, obesity patients and weight losing crowds, and has wide practical application values.
Description
Technical field
The present invention relates to make snow chrysanthemum polysaccharide tea treasure's technical field.Specifically, the present invention relates to a kind of make have anti-oxidant, hypoglycemic, the precious goods of snow chrysanthemum polysaccharide tea of lipopenicillinase.
Background technology
Snow chrysanthemum is the double-colored golden wave of one that produce in Kunlun Mountain of Xinjiang area, is grown in 2600 meters of above Kunluns of height above sea level, is that current Xinjiang is unique equally celebrated for their achievements with saussurea involucrata, has the rare psychrophyte of unique effects.Snow daisy_petal part is golden yellow, and ball-type pistil is brown, and annual August is in full bloom, and the florescence is very brief, and growth area is little, and growing environment is special, and output is minimum, precious.Have a sweet temper, hypotensive, blood fat effectively.The snow chrysanthemum of highest ranking refers to the secret the Kunlun of the dimension of the extremely frigid zones that grows in 3000 meters of left and right of height above sea level snow chrysanthemum, is referred to as " high height above sea level the Kunlun snow chrysanthemum ".
Chang Yinxue chrysanthemum tea, there are magical step-down, effect for reducing fat, and have well clear liver and improve vision, beneficial liver tonifying yin, beautifying face and moistering lotion, the characteristic that relaxes bowel, the symptoms such as the visual fatigue that excess eye-using is caused and the liver injury that causes of drinking, there is certain mitigation, and before sleeping, drink this tea and can not have a sleepless night, there is on the contrary the effect of calming the nerves.Therefore, how to make the active ingredient of avenging chrysanthemum incorporate in water fully, to make to avenge, chrysanthemum is fully utilized and the nutritional labeling that makes to avenge chrysanthemum is absorbed by the body utilizes the development to snow chrysanthemum to have great importance.
Mostly snow chrysanthemum is to select the mineral water of soft in the time brewing, and snow chrysanthemum soup look is amber redness, is current unique red soup chrysanthemum tea, the existing chrysanthemum perfume (or spice) of its taste, has again the taste of pekoe concurrently, and smell delicate fragrance is without cease, the sweet silk floss of entrance is continuous, and time glycol is thick, and the sense of taste is outstanding.
Summary of the invention
Drunk as tea for current snow chrysanthemum major part in Xinjiang people's life, seldom by the reality of deep processing, and the present research major part for snow chrysanthemum is to concentrate on a certain composition in snow chrysanthemum, as flavones, but in human body, to work be the effect common due to multiple nutrients material to a kind of food.Snow chrysanthemum tea treasure in the present invention is by technology such as ultrasonic, enzymolysis, and the nutritional labeling that makes to avenge in chrysanthemum more incorporates in water, so that the nutriment utilization that is absorbed by the body fully of snow chrysanthemum, and does not cause the waste of resource.
The present invention realizes by the technical scheme providing below:
Taking snow chrysanthemum as raw material, through selecting, impurity elimination, pulverizing, the snow inulin end of system, utilizes the auxiliary biological complex enzyme solution technology of ultrasonic technique to make to avenge the clasmatosis of chrysanthemum, makes to greatest extent intracellular nutriment be dissolved out fully.Utilizing spray drying technology, solution is become to granular powder, be convenient to be drunk and preserve, thereby having of system is anti-oxidant, the effect of hypoglycemic.
The present invention specifically provides a kind of method of making snow chrysanthemum polysaccharide tea treasure, and concrete steps are as follows:
(1) select that neat and well spaced degree is high, flower bone is full, pistil petal color and luster is orange, containing the drying snow chrysanthemum of the impurity of leaf stalk, pistil smalls or other non-snow chrysanthemum classes, by broken the snow inulin of choosing, the granularity at the snow inulin end after pulverizing is at 100 orders.
(2) water is joined in the snow inulin end of step (1) by weight the ratio of 1:30, stir with glass bar, put in thermostat water bath preheating half an hour, the temperature of thermostat water bath is 55 DEG C.
(3) above-mentioned steps (2) soln using ultrasonic disruption after treatment instrument is carried out to ultrasonic processing, temperature when ultrasonic processing is 55 DEG C, time 15min, power 160w.
(4) above-mentioned steps (3) snow after treatment chrysanthemum solution is poured in enzyme reactor, in enzyme reactor, adds cellulase, pectase and papain; The consumption of cellulase is 1% of snow chrysanthemum powder quality, and the consumption of pectase is 1% of snow chrysanthemum powder quality, and the consumption of papain is 1.5% of snow chrysanthemum powder quality, and the time of enzymatic reaction is 3h, 55 DEG C of temperature, pH value nature.
(5) enzyme reaction finish after by the enzyme processing of going out of the snow chrysanthemum solution in enzyme reactor, the temperature of the enzyme that goes out is 100 DEG C, the time is 5min, makes the enzyme deactivation in solution.
(6) leaching liquor after step (5) is finished carries out centrifugal treating, and the centrifugal time is 15min, and rotating speed is 3500r/min, obtains supernatant.
(7) spraying is dry: in supernatant, add beta-schardinger dextrin-, maltodextrin, the mixed quality of beta-schardinger dextrin-and maltodextrin is 2:3 with the mass ratio of snow inulin end solid content, sprays dry; Beta-schardinger dextrin-and maltodextrin are by quality 1:1 composition; 170～180 DEG C of EATs, 78～87 DEG C of temperature of outgoing airs, dry rear material moisture≤5%.
(8) the dried snow chrysanthemum polysaccharide tea treasure that sprays packs and is finished product.
The invention provides a kind of snow chrysanthemum polysaccharide tea treasure, snow chrysanthemum polysaccharide tea treasure is by avenging chrysanthemum polysaccharide powder 55%-60%, and beta-schardinger dextrin-15%-20% and maltodextrin 15%-20% composition, obtain by the above-mentioned preparation technology who provides.
The ultrasonic technique that the present invention adopts, biological complex enzyme solution technology, the technology such as spraying is dry, these sport technique segments are all the common technical measures in this area from single technical standpoint, but obtain provided by the invention " natural, healthy, nutrition, safety " the precious product typicalness of snow chrysanthemum polysaccharide tea, its integrated artistic has upper and lower one, tight indivisible feature, jointly by ultrasonic technique and biological complex enzyme solution technology, the cell of snow chrysanthemum is carried out to fragmentation, making to avenge polysaccharide material in chrysanthemum is leached out fully, reach making full use of of snow chrysanthemum, and do not cause snow chrysanthemum waste.
In the present invention, the ultramicro grinding of employing, for avoiding occurring overbreak, can obtain the superfine powder that particle diameter is evenly distributed, and has increased to a great extent the specific area of micro mist simultaneously, makes the also corresponding increase such as adsorptivity, dissolubility; In crushing process, can not produce hot-spot phenomenon, speed is fast, and the present invention utilizes ultramicro grinding to retain to greatest extent the bioactive ingredients of snow chrysanthemum polysaccharide powder, and the nutritional labeling in snow chrysanthemum is more easily directly absorbed by human body stomach, when water Instant Drinks, become solution shape, without precipitation.
In the present invention, the mixture of selecting malto-oligosaccharide to be formed by the carbohydrate of different polymerization degree in the precious formula of snow chrysanthemum polysaccharide tea, in the enteron aisle of animal, be not hydrolyzed the enzymatic hydrolysis system of isomalto-oligosaccharide, therefore, not digested absorption, is preferably Bifidobacterium in large intestine and utilizes but directly enter, thus, isomalto-oligosaccharide is the MF of Bifidobacterium, the Oilgosaccharkdes of conventionally saying; Due to beneficial bacterium in its energy high efficiently multiplying enteron aisle, be also referred to as prebiotics again; Isomalto-oligosaccharide is entering after human body intestinal canal, decomposes for the beneficial bacterium in enteron aisle absorbs, and produces a large amount of SCFAs, can stimulate intestines peristalsis, stop the invasion of pernicious bacteria, treat constipation thereby reach anti-, suppress the propagation of harmful intestinal tract flora, improve intestinal environment and protection enteron aisle; As a kind of good water-soluble dietary fiber, can effectively reduce the quantity of serum cholesterol, triglycerides, free fatty, have a better role for a series of angiocardiopathies such as the hypertension, the artery sclerosis etc. that cause because blood fat is high.The present invention confirms to select the standby snow of maltodextrin preparation chrysanthemum polysaccharide tea treasure by test, and the snow chrysanthemum polysaccharide tea treasure of preparation has significant hypoglycemic effect, and diabetic, obesity patient and people to lose weight are had to therapeutic action.
In the present invention, the rate of drying adopting is fast, snow chrysanthemum leaching liquor is in hot-air flow, moment just can be evaporated the moisture content of 95%-98%, complete and only need the several seconds drying time, dry run material heated time is short, material temperature is low, and the activity of bioactivator can retain well, and product grain degree is little and even, there are good dispersiveness and instant capacity; Production process is simplified, convenient operation and control.After dry, do not need to pulverize and screening, reduce production process, improve product purity; To product cut size, bulk density, moisture content, can adjust by changing operating condition within the specific limits, and control and management is all very convenient.
By implementing the concrete summary of the invention of the present invention, can reach following effect:
(1) major ingredient of the snow chrysanthemum polysaccharide tea treasure in the present invention snow chrysanthemum is by utilizing ultrasonic technology and biological complex enzyme solution technology, the polysaccharide material that makes to avenge in chrysanthemum is dissolved out fully, snow chrysanthemum raw material is fully utilized, product specification and added value have been improved simultaneously, lengthening manufacturing chain, has increased the comprehensive benefit of industry.
(2) the present invention is by the precious preparation technology of snow chrysanthemum polysaccharide tea, and the snow chrysanthemum polysaccharide powder that to obtain overall appearance be brownish black, has good processing characteristics, trophic function and physiologically active, meets the propensity to consume of current " natural, healthy, nutrition, safety ".In allotment, add maltodextrin, both can be used as water-soluble dietary fiber, balanced nutritious, can improve again the brew of product.
(3) snow chrysanthemum polysaccharide tea treasure's antioxidation: measured the removing ability of snow chrysanthemum polysaccharide tea treasure to free radical DPPH, hydroxyl radical free radical, and snow chrysanthemum polysaccharide tea treasure's reducing power (Prussian blue method), with Vc in contrast, although snow chrysanthemum polysaccharide tea treasure, avenges chrysanthemum polysaccharide tea treasure free radical DPPH, hydroxyl radical free radical is had to certain removing ability and certain reducing power lower than Vc in the ability of this three aspects:.
(4) the present invention is by a series of food processing technologies to Xinjiang snow chrysanthemum, finally be prepared into snow chrysanthemum polysaccharide tea treasure, reach a conclusion by rat functional experiment: snow chrysanthemum polysaccharide tea treasure provided by the invention has hypoglycemic effect, diabetic, obesity patient and people to lose weight are had to therapeutic action, the precious product of snow chrysanthemum polysaccharide tea prepared by the present invention has obvious low sugar, low fat, characteristic low in calories, precious blood lipid-reducing blood sugar-decreasing effect of snow chrysanthemum polysaccharide tea of preparing through scientific experiment is remarkable, has the using value of extensive reality.
Detailed description of the invention
, for embodiment, the present invention is described below, still, the present invention is not limited to following embodiment.In addition, in following explanation, if no special instructions, % all refers to mass percent, by m/m.
Instrument and equipment: pulverizer, thermostat water bath, enzyme reactor, spray drying device, electronic balance (0.0001g), disscting instrument etc.
Reagent adopts: snow chrysanthemum, papain, cellulase, pectase, maltodextrin, beta-schardinger dextrin-, distilled water etc.; TC, TG, LDL-C, HDL-C kit are purchased from Nanjing and build up Bioengineering Research Institute, and it is pure that other reagent is domestic analysis; Test small white mouse, 40 of rats are all purchased in Xinjiang Medicine University and zoopery center, Xinjiang Uygur Autonomous Regions.
All reagent, instrument, the raw and auxiliary material in the present invention, selected are all well known selecting, but do not limit enforcement of the present invention, and other reagent more well known in the art and equipment are all applicable to the enforcement of the following embodiment of the present invention.
embodiment mono-: snow chrysanthemum polysaccharide tea treasure's preparation
(1) select that neat and well spaced degree is high, flower bone is full, pistil petal color and luster is orange, containing the drying snow chrysanthemum of the impurity of leaf stalk, pistil smalls or other non-snow chrysanthemum classes, by broken the snow inulin of choosing, the granularity at the snow inulin end after pulverizing is at 100 orders.
(2) water is joined in the snow inulin end of step (1) by weight the ratio of 1:30, stir with glass bar, put in thermostat water bath preheating half an hour, the temperature of thermostat water bath is 55 DEG C.
(3) above-mentioned steps (2) soln using ultrasonic disruption after treatment instrument is carried out to ultrasonic processing, temperature when ultrasonic processing is 55 DEG C, time 15min, power 160w.
(4) above-mentioned steps (3) snow after treatment chrysanthemum solution is poured in enzyme reactor, in enzyme reactor, adds cellulase, pectase and papain; The consumption of cellulase is 1% of snow chrysanthemum powder quality, and the consumption of pectase is 1% of snow chrysanthemum powder quality, and the consumption of papain is 1.5% of snow chrysanthemum powder quality, and the time of enzymatic reaction is 3h, 55 DEG C of temperature, pH value nature.
(5) enzyme reaction finish after by the enzyme processing of going out of the snow chrysanthemum solution in enzyme reactor, the temperature of the enzyme that goes out is 100 DEG C, the time is 5min, makes the enzyme deactivation in solution.
(6) leaching liquor after step (5) is finished carries out centrifugal treating, and the centrifugal time is 15min, and rotating speed is 3500r/min, obtains supernatant.
(7) spraying is dry: in supernatant, add beta-schardinger dextrin-, maltodextrin, the mixed quality of beta-schardinger dextrin-and maltodextrin is 2:3 with the mass ratio of snow inulin end solid content, sprays dry; Beta-schardinger dextrin-and maltodextrin are by quality 1:1 composition; 170～180 DEG C of EATs, 78～87 DEG C of temperature of outgoing airs, dry rear material moisture≤5%.
(8) the dried snow chrysanthemum polysaccharide tea treasure that sprays packs and is finished product.
The snow chrysanthemum polysaccharide tea treasure who prepares through above-mentioned technique is by avenging chrysanthemum polysaccharide powder 55%-60%, and beta-schardinger dextrin-15%-20% and maltodextrin 15%-20% form.
embodiment bis-: the dry preparation snow of spraying chrysanthemum polysaccharide tea treasure's technical study
By the each parameter when dry of spraying has been done to detailed research to the impact of spray effect, to reach the drying process with atomizing parameter object of optimizing snow chrysanthemum polysaccharide tea treasure.
1. the impact of drying aid on snow chrysanthemum polysaccharide tea treasure, determine drying aid: when the drying snow of spray drying process chrysanthemum material, can produce glutinous wall situation, the impact of sticky wall when dry for fear of spraying on snow inulin quality and yield, so add drying aid before spraying is dry in snow inulin feed liquid, it is alternative drying aid that maltodextrin, beta-schardinger dextrin-are selected in test.Snow inulin solid content and drying aid ratio select respectively 1:3,1.5:3,2:3, tetra-kinds of ratios of 3:3 to carry out spray drying experiment, spray condition is: 170～180 DEG C of EATs, 80～85 DEG C of temperature of outgoing airs, rotating speed 35000r/min, charging rate is 28 mL/min.Testing index is aberration (Hunter L value, Hunter b value, Hunter a value), water content, observes snow chrysanthemum polysaccharide tea treasure's glutinous wall situation.
2. drying process with atomizing parameter optimization: probe into the impact of drying process with atomizing import and export temperature on the precious moisture of snow chrysanthemum polysaccharide tea and color and luster, moisture, color and luster (Hunter L value, Hunter a value, Hunter b value), the glutinous wall degree of observation in snow survey chrysanthemum polysaccharide tea treasure.
3. input concentration is on spray-dired impact: by Vacuum Concentration, snow chrysanthemum polysaccharide liquid is condensed into variable concentrations, sprays dry, survey moisture content, color and luster.
4. the dry response surface experimental design of spraying: application responds face method is carried out the response surface analysis (17 groups of experiments) of three factors to inlet temperature, outlet temperature, material concentration, detect moisture, Hunter L value, Hunter a value, Hunter b value in sample.
embodiment tri-: effect experiment of snow chrysanthemum polysaccharide tea treasure
1, determining of rat model:
Modeling condition is set up aspect, adopt STZ+ high lipid food method to make N-shaped diabetes rat model, but in modeling process, the analysis of key influence factor mainly concentrates on STZ injection volume and high fat word material proportioning aspect; The particular problems such as the current international selection in high fat nursing time, STZ ID, STZ injection site, rat sex, rat body weight, rat age, also ununified standard, and the design of these aspects is also to affect animal model to become the key factor of mould rate, stability, thereby experimental result is played to important effect.
1.1 STZ injection volume aspects: have and studies show that the concentration of the suitable use of single-bolus high-dose injection STZ is 40-60mg/kg, have researcher to think that it is optimal dose that STZ injects 50 mg/kg: multiple low dose injection STZ suitable concentration is 15-20 mg/kg; In conjunction with STZ injection volume in forefathers' research and induction success rate, the dosage that the present invention chooses is 55 mg/kg.
1.2 modeling rat sex selection aspects: the researchers such as Morimoto in 2005 find in experiment that sex is chosen in aspect STZ induction diabetes rat film forming and can make a difference and affect.The stability of blood sugar is subject to the impact of experimental rat sex, 14 days glucostasis after male rat lumbar injection STZ, after relative female rats lumbar injection STZ, after 4 weeks, blood sugar is just stable, and follow-up study infers that the rear estrogenic level of growing up can produce certain impact to glycolipid metabolism, therefore selects male mouse modeling to disturb still less subsequent experimental.After the research of Morimoto, domestic researcher has also carried out the research of this direction in a deep going way, the important function that further clear and definite rat sex plays in N-shaped diabetes rat model.Therefore in conjunction with forefathers' research, the present invention's design should select male rat as experimental subjects.
The body weight selection aspect of 1.3 rats: have researcher that experimental rat is divided with different weight gradient, the preparation of type ii diabetes rat model is carried out in then grouping, result is that body weight is in 160-180g, 181-200g, 201g-220g, 221g-240g, 241g-260g, six groups of rats of 261g-280g, body weight not only becomes mould rate the highest at 220-240g with two groups of rats of 241-260g, and the death rate is extremely low.First this result has embodied rat body weight has certain influence to carrying out of modeling and subsequent experimental, next has shown the accuracy in order to increase experiment, in the experimental design procedure of type ii diabetes rat correlative study, the body weight of rat also should be served as one of considerations; This experiment, before modeling, by regulating the nursing cycle, is tried one's best body weight control in 220-240g and 241-260g two class ranges, thereby is improved modeling success rate, makes experimental result more reliable.The age aspect of rat: forefathers' research relates to rat the susceptibility of STZ is had to age-dependent, and the therefore experimental subjects of modeling, should select adult rat.Compared to young mouse, adult rat modeling has higher success rate.Therefore this experimental subjects, chooses adult rat.Comprehensive every factor that affects modeling success rate, it is male adult rat that the present invention tests the experimental rat of choosing.
Before 1.4 modelings, body weight is controlled within the scope of 220-240g or 241-260g, and STZ injection volume is single intraperitoneal injection 55mg/kg; Due to the regulating action of this experiment fat disorder that mainly research active material causes the concurrent hyperlipidemia of type ii diabetes, therefore experimental technique adopts the method for STZ (induction type ii diabetes)+high lipid food (induction hyperlipidemia), after modeling success, after gastric infusion certain time, using the variation of blood fat, blood sugar level etc. as test index, thereby observe snow chrysanthemum polysaccharide tea treasure, whether rat body weight, blood sugar level and fat disorder be there is to regulating action.
2, reagent: TC, TG, LDL-C, HDL-C kit are purchased from Nanjing and build up Bioengineering Research Institute, and it is pure that other reagent is domestic analysis.
3, animal experiment method
3.1 animals: 40 of clean level male SD rats, body weight 90 1 110g.
3.2 feeds: basal feed is that market is common; High lipid food is made up of 90% basal feed, 10% lard, and fresh lard is purchased near supermarket city.
3.3 animal grouping and experimental techniques.
40 rats adapt to feed after one week at basal feed, get at random 8 continue feeding basal feed by body weight, and as normal group (N), " after modeling success, modeling group rat is divided into four groups by body weight to all the other equal feeding high lipid foods at random, 8 every group.First group of high lipid food group, HF feeding high lipid food; Second group of precious group of high lipid food snow chrysanthemum polysaccharide tea, F mono-HF feeding is containing 5% snow chrysanthemum polysaccharide tea treasure's high lipid food, i.e. 95% high lipid food+5% snow chrysanthemum polysaccharide tea treasure (low dose group); The 3rd group of basal feed group, c feeding basal feed one; The 4th group of precious group of basal feed snow chrysanthemum polysaccharide tea, F-C feeding is containing 10% snow chrysanthemum polysaccharide tea treasure's basal feed, i.e. 9,0% high lipid food+10% snow chrysanthemum polysaccharide tea treasure (low dose group).
Each treated animal list cage is raised, and indoor temperature is controlled at 22 1 25 DEG C, and humidity is controlled at the dark control automatically of 40 1 60%, 12h illumination 12h, and ad lib water inlet, measures body weight and food ration for every two days once; After 24 days, each group rat fasting 24h, after claiming end-body heavy, broken end is got blood, separation of serum, reference reagent box description carries out the mensuration of serum total cholesterol (TC), triglycerides (TG), LDL-C (LDL-C), HDL-C (HDL-C) content.Dissect rat, visually observe the calm situation of rat body fat and internal organs situation of change, calculate AI.
3.4 statistical procedures: TC, TG, LDL-C, HDL-C all measure by kit requirement: food utilization=body weight recruitment/food ration × 100%; Fat coefficient=fatty gross weight/body weight × 100%; AI AI=(TC-HDL-C)/HDL-C; Adopt SPSSl6.0 software to carry out one-way analysis of variance.
3.5 result
3.5.1 animal used as test obese model
The body weight there was no significant difference of the front two groups of rats of modeling, after modeling, the rat body weight of modeling group is apparently higher than normal rats body weight (P<0.01), as table 1.
Table 1: the foundation of obesity rat model
| Group | Rat quantity (only) | Body weight (g) before modeling | Body weight after modeling (g) |
| Normal group | 8 | 90.2±2.4 | 267.8±4.1 |
| Modeling group | 32 | 89.7±2.3 | 289.2±2.54 |
3.5.2 the impact on rat food ration, body weight and weightening finish
As shown in table 2, in experimentation, each group rat shows no obvious abnormalities, and diet activity in order.Each group rat body weight no significant difference before experiment.When experiment finishes, compared with HF group, F-HF group rat body weight significantly reduces (P<0.05); C group, F-C group extremely significantly reduce (P<0.01); The weightening finish of each group rat all extremely significantly reduces (P<0.01); F-HF group, C group, the weightening finish of F-C group rat body weight reduce successively.Illustrate that the ingesting intervention including snow chrysanthemum polysaccharide tea treasure can significantly reduce body weight and the weightening finish of nutritive obesity in rats, and the adjustment best results of ingesting in conjunction with low fat with snow chrysanthemum polysaccharide tea treasure.
F-HF group is with HF group without significant difference, and F-C group without significant difference, illustrates that snow chrysanthemum polysaccharide tea treasure has no significant effect the food ration of experimental rat with C group, and the precious effect that reduces body weight of snow chrysanthemum polysaccharide tea is not by reducing rat, the intake of food to be reached.
Table 2: the impact of snow chrysanthemum polysaccharide tea treasure on rat food ration, body weight and weightening finish
3.5.3 the impact on rat fat index and AI
As shown in table 3, compared with HF group, F-HF group, C group, F-C group rat TC, LDL-C, AI extremely significantly reduce (P<0.01); HDL-C extremely significantly raise (P<0.01); Wherein F-HF group, F-C group rat HDL-C are higher than C group; F-HF group, F-C group rat TG extremely significantly reduce (P<0.01), C group rat TG is without marked change, illustrate and comprise that snow chrysanthemum polysaccharide tea treasure and diet adjustment can all significantly reduce TC, LDL-C content in rat body, improve HDL-C content, effectively improve blood lipid level in rat body, reduce and suffer from atherosclerotic risk; The reduction of snow chrysanthemum polysaccharide tea treasure to TG content in rat body and the rising effect of HDL-C content are better than diet adjustment.
Table 3: the impact of snow chrysanthemum polysaccharide tea treasure on rat blood serum index
conclusion:as seen from the experiment, the snow chrysanthemum polysaccharide tea treasure who adopts the present invention to prepare is the same with diet adjustment, can significantly reduce the body weight of nutritive obesity in rats, suppresses the accumulation of rat body fat, and nutritive obesity in rats is had to good fat-reducing effect; The precious significantly reduction of snow chrysanthemum polysaccharide tea Serum TC, TG, LDL-C content prepared by the present invention, significantly improve rat blood serum HDL-C content, has good lipid-lowering effect; The rising effect of the reduction of snow chrysanthemum polysaccharide tea treasure prepared by the present invention to rat blood serum TG content and HDL-C content is especially remarkable, is better than diet adjustment.
Result of the test shows: snow chrysanthemum polysaccharide tea treasure prepared by the present invention has good functions of lowering blood-fat and reducing weight to nutritive obesity in rats, and this provides a kind of new way, the further research but the aspects such as its mechanism of action need for developing natural lowering blood-fat and reducing weight product.To the bioactive further investigation of snow chrysanthemum, not only can develop snow chrysanthemum and utilize new way, improve snow chrysanthemum resource utilization, can also improve snow chrysanthemum producing region economic benefit, there is important social effect.
More than setting forth the embodiment in the present invention, is not that the present invention is done to other formal restriction, and these professional personnel can change equivalent embodiment to the technology contents of above-mentioned elaboration.But as long as not departing from technical solution of the present invention content, according to technical spirit of the present invention, just above example is carried out to simple modification, equivalent variations and remodeling, still belong to the protection domain of the technology of the present invention.
Claims (2)
1. a method of making snow chrysanthemum polysaccharide tea treasure, is characterized in that, described method step is as follows:
(1) select that neat and well spaced degree is high, flower bone is full, pistil petal color and luster is orange, containing the drying snow chrysanthemum of the impurity of leaf stalk, pistil smalls or other non-snow chrysanthemum classes, by broken the snow inulin of choosing, the granularity at the snow inulin end after pulverizing is at 100 orders;
(2) water is joined in the snow inulin end of step (1) by weight the ratio of 1:30, stir with glass bar, put in thermostat water bath preheating half an hour, the temperature of thermostat water bath is 55 DEG C;
(3) above-mentioned steps (2) soln using ultrasonic disruption after treatment instrument is carried out to ultrasonic processing, temperature when ultrasonic processing is 55 DEG C, time 15min, power 160w;
(4) above-mentioned steps (3) snow after treatment chrysanthemum solution is poured in enzyme reactor, in enzyme reactor, adds cellulase, pectase and papain; The consumption of cellulase is 1% of snow chrysanthemum powder quality, and the consumption of pectase is 1% of snow chrysanthemum powder quality, and the consumption of papain is 1,5% of snow chrysanthemum powder quality, and the time of enzymatic reaction is 3h, 55 DEG C of temperature, pH value nature;
(5) enzyme reaction finish after by the enzyme processing of going out of the snow chrysanthemum solution in enzyme reactor, the temperature of the enzyme that goes out is 100 DEG C, the time is 5min, makes the enzyme deactivation in solution;
(6) leaching liquor after step (5) is finished carries out centrifugal treating, and the centrifugal time is 15min, and rotating speed is 3500r/min, obtains supernatant;
(7) spraying is dry: in supernatant, add beta-schardinger dextrin-, maltodextrin, the mixed quality of beta-schardinger dextrin-and maltodextrin is 2:3 with the mass ratio of snow inulin end solid content, sprays dry; Beta-schardinger dextrin-and maltodextrin are by quality 1:1 composition; 170～180 DEG C of EATs, 78～87 DEG C of temperature of outgoing airs, dry rear material moisture≤5%;
(8) the dried snow chrysanthemum polysaccharide tea treasure that sprays packs and obtains the precious finished product of snow chrysanthemum polysaccharide tea.
2. the snow chrysanthemum polysaccharide tea treasure that prepared by making snow chrysanthemum polysaccharide tea treasure's as claimed in claim 1 method, is characterized in that, described snow chrysanthemum polysaccharide tea treasure is by avenging chrysanthemum polysaccharide powder 55%-60%, and beta-schardinger dextrin-15%-20% and maltodextrin 15%-20% form.
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| CN201410250085.9A CN103976110B (en) | 2014-06-09 | 2014-06-09 | A kind of coreopsis tinctoria flower tea is precious and preparation method thereof |
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| CN104586910A (en) * | 2014-12-10 | 2015-05-06 | 新疆华铁能源科技有限公司 | Coreopsis tinctoria extract and preparation method thereof |
| CN104872333A (en) * | 2015-05-28 | 2015-09-02 | 浙江农林大学 | Manufacture method of cymose buckwheat rhizome seed tea powder |
| CN104996652A (en) * | 2015-06-01 | 2015-10-28 | 新疆生命核力高科股份有限公司 | Sanvitalia procumbens beverage and processing method thereof |
| CN107173490A (en) * | 2017-05-16 | 2017-09-19 | 吴启川 | A kind of dog bull brain tea beverage processing technology |
| CN107319057A (en) * | 2017-08-26 | 2017-11-07 | 安徽阜南常晖食品有限公司 | A kind of preparation method of stem of noble dendrobium green tea beverage |
| CN109846964A (en) * | 2019-04-16 | 2019-06-07 | 滨州医学院附属医院 | Using chrysanthemum as anti-inflammatory tea-drinking of primary raw material and preparation method thereof |
| CN115886088A (en) * | 2022-11-22 | 2023-04-04 | 深圳市深宝华城科技有限公司 | Method for extracting tea leaves by combining repeated freezing and thawing with dynamic ultrahigh-pressure micro-jet technology |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104586910A (en) * | 2014-12-10 | 2015-05-06 | 新疆华铁能源科技有限公司 | Coreopsis tinctoria extract and preparation method thereof |
| CN104872333A (en) * | 2015-05-28 | 2015-09-02 | 浙江农林大学 | Manufacture method of cymose buckwheat rhizome seed tea powder |
| CN104996652A (en) * | 2015-06-01 | 2015-10-28 | 新疆生命核力高科股份有限公司 | Sanvitalia procumbens beverage and processing method thereof |
| CN107173490A (en) * | 2017-05-16 | 2017-09-19 | 吴启川 | A kind of dog bull brain tea beverage processing technology |
| CN107319057A (en) * | 2017-08-26 | 2017-11-07 | 安徽阜南常晖食品有限公司 | A kind of preparation method of stem of noble dendrobium green tea beverage |
| CN109846964A (en) * | 2019-04-16 | 2019-06-07 | 滨州医学院附属医院 | Using chrysanthemum as anti-inflammatory tea-drinking of primary raw material and preparation method thereof |
| CN115886088A (en) * | 2022-11-22 | 2023-04-04 | 深圳市深宝华城科技有限公司 | Method for extracting tea leaves by combining repeated freezing and thawing with dynamic ultrahigh-pressure micro-jet technology |
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