CN103976110B - A kind of coreopsis tinctoria flower tea is precious and preparation method thereof - Google Patents
A kind of coreopsis tinctoria flower tea is precious and preparation method thereof Download PDFInfo
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Abstract
The invention discloses coreopsis tinctoria flower tea treasure and preparation method thereof.To avenge inulin broken, the ratio by weight 1:30 adds water to avenge in inulin end, carries out ultrasonically treated after water bath with thermostatic control, ultrasonically treated 55 DEG C, and 15min, power 160w, through enzymolysis, add cellulase, pectase and papain；The consumption of cellulase is 1%, the consumption of pectase is 1%, and the consumption of papain is 1.5%, enzymolysis 3h, 55 DEG C, go out enzyme 100 DEG C, 5min, centrifugal 15min, 3500r/min obtains supernatant, the mass ratio adding beta cyclodextrin, maltodextrin, beta cyclodextrin and the mixed quality of maltodextrin and snow inulin end solid content is 2:3, the acquisition finished product after being spray-dried.The snow chrysanthemum polysaccharide tea treasure of preparation has hypoglycemic effect, and diabetic, obesity patient and people to lose weight are had therapeutic action, has the using value of extensively reality.
Description
Technical field
The present invention relates to make the technical field that snow chrysanthemum polysaccharide tea is precious.Specifically, the present invention relates to a kind of making and have anti-oxidant, hypoglycemic, snow chrysanthemum polysaccharide tea treasure's goods of lipopenicillinase.
Background technology
Snow chrysanthemum is the double-colored golden wave of one that produced of Kunlun Mountain of Xinjiang area, is grown in the Kunlun of height above sea level more than 2600 meters, is that current Xinjiang is unique the most equally celebrated for their achievements with saussurea involucrata, has the rare psychrophyte of unique effects.Snow daisy_petal part is golden yellow, and ball-type pistil is brown, and annual August blooms, and the florescence is very brief, and growth area is little, and growing environment is special, and yield is minimum, precious.Have a sweet temper, it is possible to the most hypotensive, blood fat.The snow chrysanthemum of highest ranking refers to be grown on the secret coreopsis tinctoria of dimension of the extremely frigid zones of height above sea level about 3000 meters, referred to as " High aititude coreopsis tinctoria ".
Often drink coreopsis tinctoria flower tea, there are the step-down of mystery, effect for reducing fat, and have well clear liver and improve vision, benefit liver tonifying yin, beautifying face and moistering lotion, the characteristic that relaxes bowel, the visual fatigue that excess eye-using is caused and the symptoms such as the liver injury that causes of drinking, there is certain mitigation, and drink this tea before sleeping will not have a sleepless night, there is effect on the contrary that calm the nerves.Therefore, the active ingredient how making snow chrysanthemum sufficiently incorporates in water, in order to make snow chrysanthemum be fully utilized and make the nutritional labeling of snow chrysanthemum be absorbed by the body to utilize the development to snow chrysanthemum to have great importance.
Mostly snow chrysanthemum is to select the mineral water of soft when brewing, and avenging chrysanthemum soup look is that amber is red, is currently the only red soup chrysanthemum tea, and the existing chrysanthemum of its taste is fragrant, has again the taste of pekoe concurrently, and smell delicate fragrance is without cease, and the sweet silk floss of entrance is continuous, returns glycol thickness, and the sense of taste is outstanding.
Summary of the invention
It is to be taken as tea to drink for snow chrysanthemum major part in the life of Xinjiang people at present, seldom by the reality of deep processing, and the present research major part for snow chrysanthemum is to concentrate on a certain composition in snow chrysanthemum, such as flavones, but to work in human body be due to the common effect of multiple nutrients material to a kind of food.Coreopsis tinctoria flower tea treasure in the present invention is by technology such as ultrasonic, enzymolysis, makes the nutritional labeling in snow chrysanthemum more incorporate in water, in order to the nutriment of snow chrysanthemum is sufficiently absorbed by the body utilization, and does not cause the waste of resource.
The present invention is realized by technical scheme presented below:
With snow chrysanthemum as raw material, through selecting, impurity elimination, pulverizing, the snow inulin end of system, utilize ultrasonic technique auxiliary biological complex enzyme solution technology to make the clasmatosis of snow chrysanthemum, make intracellular nutriment be sufficiently dissolved out to greatest extent.Utilizing spray drying technology, solution is being become granular powder, it is simple to be drunk and preserve, thus having of making is anti-oxidant, the effect of hypoglycemic.
The present invention specifically provides a kind of method making snow chrysanthemum polysaccharide tea treasure, specifically comprises the following steps that
(1) select that neat and well spaced degree is high, bone is full, pistil petal color and luster is orange for flower, the drying snow chrysanthemum of impurity without leaf stalk, pistil smalls or other non-snow chrysanthemum classes, by broken for the snow inulin chosen, the granularity at the snow inulin end after pulverizing is at 100 mesh.
(2) during the ratio by weight 1:30 adds water to the snow inulin end of step (1), stirring with glass bar, preheat half an hour in putting into thermostat water bath, the temperature of thermostat water bath is 55 DEG C.
(3) it is ultrasonically treated that the solution after above-mentioned steps (2) being processed utilizes sonicator to carry out, and temperature time ultrasonically treated is 55 DEG C, time 15min, power 160w.
(4) the snow chrysanthemum solution after above-mentioned steps (3) being processed is poured in enzyme reactor, adds cellulase, pectase and papain in enzyme reactor；The consumption of cellulase is the 1% of snow chrysanthemum powder quality, and the consumption of pectase is the 1% of snow chrysanthemum powder quality, and the consumption of papain is the 1.5% of snow chrysanthemum powder quality, and the time of enzymatic reaction is 3h, temperature 55 DEG C, and pH value is natural.
(5) go out after enzyme reaction terminates ferment treatment by the snow chrysanthemum solution in enzyme reactor, and the temperature of the enzyme that goes out is 100 DEG C, and the time is 5min, makes the enzyme in solution inactivate.
(6) leaching liquor after step (5) being terminated is centrifuged processing, and the centrifugal time is 15min, and rotating speed is 3500r/min, obtains supernatant.
(7) it is spray-dried: in supernatant, add beta-schardinger dextrin, maltodextrin, beta-schardinger dextrin and the mixed quality of maltodextrin and the mass ratio avenging inulin end solid content is 2:3, be spray-dried；Beta-schardinger dextrin is formed by quality 1:1 with maltodextrin；EAT 170～180 DEG C, temperature of outgoing air 78～87 DEG C, dried material moisture≤5%.
(8) the snow chrysanthemum polysaccharide tea treasure after being spray-dried carries out packaging and is finished product.
The present invention provides a kind of snow chrysanthemum polysaccharide tea precious, and snow chrysanthemum polysaccharide tea is precious by snow chrysanthemum polysaccharide powder 55%-60%, beta-schardinger dextrin 15%-20% and maltodextrin 15%-20% composition, is obtained by the preparation technology of above-mentioned offer.
The ultrasonic technique that the present invention uses, biological complex enzyme solution technology, the technology such as spray drying, these sport technique segments are all the common technical measures in this area from single technical standpoint, but it is intended to obtain the " natural of present invention offer, healthy, nutrition, safety " snow chrysanthemum polysaccharide tea treasure's product typicalness, its integrated artistic has one up and down, tight indivisible feature, commonly through ultrasonic technique and biological complex enzyme solution technology, the cell of snow chrysanthemum is crushed, polysaccharide material in snow chrysanthemum is made sufficiently to be leached out, reach to avenge making full use of of chrysanthemum, and do not cause the waste of snow chrysanthemum.
In the present invention, the ultramicro grinding of employing, broken for avoiding the occurrence of, the superfine powder of even particle size distribution can be obtained, largely add the specific surface area of micro mist simultaneously, make adsorptivity, dissolubility etc. increase the most accordingly；Hot-spot phenomenon will not be produced in crushing process, speed is fast, and the present invention utilizes ultramicro grinding to retain the bioactive ingredients of snow chrysanthemum polysaccharide powder to greatest extent, and the nutritional labeling in snow chrysanthemum is easier to directly be absorbed by human body stomach, solution shape is become, without precipitation during with water Instant Drinks.
In the present invention, snow chrysanthemum polysaccharide tea treasure formula is selected the mixture that malto-oligosaccharide is made up of the carbohydrate of different polymerization degree, the enteron aisle of animal does not hydrolyze the enzymatic hydrolysis system of isomalto-oligosaccharide, therefore, the most digested absorption, but be directly entered big enteral and be preferably Bifidobacterium and utilized, thus, isomalto-oligosaccharide is the MF of Bifidobacterium, the Oilgosaccharkdes i.e. generally said；Again due to beneficial bacterium, also referred to as prebiotics in its energy high efficiently multiplying enteron aisle；Isomalto-oligosaccharide, after entering human body intestinal canal, absorbs for the beneficial bacterium in enteron aisle and decomposes, produce substantial amounts of SCFA; intestines peristalsis can be stimulated, stop the invasion of pernicious bacteria, thus reach anti-and treat constipation; the propagation of suppression harmful intestinal tract flora, improves intestinal environment and protection enteron aisle；As a kind of excellent water-soluble dietary fiber, can effectively reduce the quantity of serum cholesterol, triglycerides, free fatty, a series of angiocardiopathies such as the hypertension caused because blood fat is high, artery sclerosis are had a better role.The present invention confirms to select maltodextrin preparation standby snow chrysanthemum polysaccharide tea precious by test, and the snow chrysanthemum polysaccharide tea treasure of preparation has significant hypoglycemic effect, diabetic, obesity patient and people to lose weight are had therapeutic action.
In the present invention, the rate of drying used is fast, snow chrysanthemum leaching liquor is in hot-air flow, moment just can evaporate the moisture content of 95%-98%, completing only to need drying time the several seconds, dry run material heated time is short, material temperature is low, and the activity of bioactivator can retain well, and product grain degree is little and uniform, there are well dispersiveness and instant capacity；Production process simplifies, convenient operation and control.It is not required to after drying pulverize and screening, reduces production process, improve product purity；To product cut size, bulk density, moisture content, can be adjusted by changing operating condition within the specific limits, control and manage the most very convenient.
By implementing the concrete summary of the invention of the present invention, following effect can be reached:
(1) the major ingredient snow chrysanthemum that the snow chrysanthemum polysaccharide tea in the present invention is precious is by utilizing ultrasonic technology and biological complex enzyme solution technology, the polysaccharide material in snow chrysanthemum is made sufficiently to be dissolved out, snow chrysanthemum raw material is fully utilized, improve product specification and added value simultaneously, lengthening manufacturing chain, adds the comprehensive benefit of industry.
(2) present invention is by snow chrysanthemum polysaccharide tea treasure preparation technology, it is thus achieved that overall appearance is the snow chrysanthemum polysaccharide powder of brownish black, has good processing characteristics, trophic function and physiologically active, meets the propensity to consume of current " natural, healthy, nutrition, safety ".Allotment adds maltodextrin, both can be balanced nutritious as water-soluble dietary fiber, the brew of product can be improved again.
(3) antioxidation that snow chrysanthemum polysaccharide tea is precious: determine snow chrysanthemum polysaccharide tea precious to free radical DPPH, the Scavenging activity of hydroxyl radical free radical, and the reducing power (Prussian blue method) that snow chrysanthemum polysaccharide tea is precious, using Vc as comparison, although the precious ability at this three aspect of snow chrysanthemum polysaccharide tea is less than Vc, but snow chrysanthemum polysaccharide tea treasure has certain Scavenging activity and certain reducing power to free radical DPPH, hydroxyl radical free radical.
(4) present invention is by a series of food processing technologies to Xinjiang snow chrysanthemum, finally it is prepared as avenging chrysanthemum polysaccharide tea precious, conclude that the snow chrysanthemum polysaccharide tea treasure that the present invention provides has hypoglycemic effect by rat functional experiment, diabetic, obesity patient and people to lose weight there is therapeutic action, snow chrysanthemum polysaccharide tea treasure's product prepared by the present invention has obvious low sugar, low fat, characteristic low in calories, the snow chrysanthemum polysaccharide tea treasure's blood lipid-reducing blood sugar-decreasing effect prepared through scientific experiment is notable, has the using value of extensively reality.
Detailed description of the invention
Below, for embodiment, the present invention is described, but, the present invention is not limited to following embodiment.It addition, in following explanation, if no special instructions, % all refers to mass percent, i.e. based on m/m.
Instrument and equipment: pulverizer, thermostat water bath, enzyme reactor, spray drying device, electronic balance (0.0001g), disscting instrument etc..
Reagent uses: snow chrysanthemum, papain, cellulase, pectase, maltodextrin, beta-schardinger dextrin, distilled water etc.；TC, TG, LDL-C, HDL-C kit is purchased from Nanjing and builds up Bioengineering Research Institute, and it is pure that other reagent is domestic analysis；Test small white mouse, rat 40 are the most all purchased in Xinjiang Medicine University and Xinjiang Uygur Autonomous Regions's animal experimental center.
In the present invention, all reagent of selection, instrument, raw and auxiliary material are all to it is well known that selection, but are not intended to the enforcement of the present invention, and other reagent more well known in the art and equipment are applied both to the enforcement of implementation below of the present invention.
Embodiment one: the preparation that snow chrysanthemum polysaccharide tea is precious
(1) select that neat and well spaced degree is high, bone is full, pistil petal color and luster is orange for flower, the drying snow chrysanthemum of impurity without leaf stalk, pistil smalls or other non-snow chrysanthemum classes, by broken for the snow inulin chosen, the granularity at the snow inulin end after pulverizing is at 100 mesh.
(2) during the ratio by weight 1:30 adds water to the snow inulin end of step (1), stirring with glass bar, preheat half an hour in putting into thermostat water bath, the temperature of thermostat water bath is 55 DEG C.
(3) it is ultrasonically treated that the solution after above-mentioned steps (2) being processed utilizes sonicator to carry out, and temperature time ultrasonically treated is 55 DEG C, time 15min, power 160w.
(4) the snow chrysanthemum solution after above-mentioned steps (3) being processed is poured in enzyme reactor, adds cellulase, pectase and papain in enzyme reactor；The consumption of cellulase is the 1% of snow chrysanthemum powder quality, and the consumption of pectase is the 1% of snow chrysanthemum powder quality, and the consumption of papain is the 1.5% of snow chrysanthemum powder quality, and the time of enzymatic reaction is 3h, temperature 55 DEG C, and pH value is natural.
(5) go out after enzyme reaction terminates ferment treatment by the snow chrysanthemum solution in enzyme reactor, and the temperature of the enzyme that goes out is 100 DEG C, and the time is 5min, makes the enzyme in solution inactivate.
(6) leaching liquor after step (5) being terminated is centrifuged processing, and the centrifugal time is 15min, and rotating speed is 3500r/min, obtains supernatant.
(7) it is spray-dried: in supernatant, add beta-schardinger dextrin, maltodextrin, beta-schardinger dextrin and the mixed quality of maltodextrin and the mass ratio avenging inulin end solid content is 2:3, be spray-dried；Beta-schardinger dextrin is formed by quality 1:1 with maltodextrin；EAT 170～180 DEG C, temperature of outgoing air 78～87 DEG C, dried material moisture≤5%.
(8) the snow chrysanthemum polysaccharide tea treasure after being spray-dried carries out packaging and is finished product.
The snow chrysanthemum polysaccharide tea prepared through above-mentioned technique is precious by snow chrysanthemum polysaccharide powder 55%-60%, beta-schardinger dextrin 15%-20% and maltodextrin 15%-20% composition.
Embodiment two: be spray-dried the technical study that preparation snow chrysanthemum polysaccharide tea is precious
By the impact of spray effect has been made detailed research by each parameter when being spray-dried, to reach to optimize the drying process with atomizing parameter purpose that snow chrysanthemum polysaccharide tea is precious.
1. the impact that drying aid is precious on snow chrysanthemum polysaccharide tea, determine drying aid: during spray drying process drying snow chrysanthemum material, glutinous wall situation can be produced, viscous wall during in order to avoid being spray-dried is on snow inulin quality and the impact of yield, so adding drying aid before Pen Wuganzaoing in snow inulin feed liquid, test selects maltodextrin, beta-schardinger dextrin to be alternative drying aid.Snow inulin solid content selects tetra-kinds of ratios of 1:3,1.5:3,2:3,3:3 to carry out spray drying experiment with drying aid ratio respectively, spray condition is: EAT 170～180 DEG C, temperature of outgoing air 80～85 DEG C, rotating speed 35000r/min, charging rate is 28 mL/min.Testing index is aberration (Hunter L value, Hunter b value, Hunter a value), water content, observes the glutinous wall situation that snow chrysanthemum polysaccharide tea is precious.
2. drying process with atomizing parameter optimization: probe into drying process with atomizing import and export temperature to snow chrysanthemum polysaccharide tea treasure's moisture and the impact of color and luster, moisture, color and luster (Hunter in snow survey chrysanthemum polysaccharide tea treasure
L value, Hunter a value, Hunter
B value), observe glutinous wall degree.
3. the input concentration impact on being spray-dried: by being concentrated in vacuo, snow chrysanthemum polysaccharide liquid is condensed into variable concentrations, is spray-dried, surveys moisture content, color and luster.
4. it is spray-dried response surface experiments design: application response phase method carries out the response surface analysis (17 groups of experiments) of three factors to inlet temperature, outlet temperature, material concentration, detects moisture, Hunter L value, Hunter in sample
A value, Hunter b value.
Embodiment three: the Efficacy experiments that snow chrysanthemum polysaccharide tea is precious
1, the determination of rat model:
Modeling condition sets up aspect, uses STZ+ high lipid food method to make N-shaped diabetes rat model, but during modeling, the analysis of key influence factor is concentrated mainly on STZ injection volume and high fat word material proportioning aspect；The most international in particular problems such as high fat nursing time, STZ injection dosage, STZ injection site, the selection of rat sex, rat body weight, rat ages, the most unified standard, and the design in terms of these is also to affect animal model to become the key factor of mould rate, stability, thus experimental result is played an important role.
1.1
STZ injection volume aspect: having the research display single-bolus high-dose injection concentration that suitably uses of STZ is 40-60mg/kg, having researcher to think, STZ injects 50 mg/kg is optimal dose: multiple low dose injection STZ suitable concentration is 15-20 mg/kg；STZ injection volume and induction success rate in studying in conjunction with forefathers, the dosage that the present invention chooses is 55 mg/kg.
1.2 modeling rat Sex preference aspects: the researchers such as Morimoto in 2005 find that Sex preference can make a difference and affect in terms of STZ induced diabetic rats film forming in an experiment.The stability of blood sugar is affected by experimental rat sex, 14 days glucostasis after male rat lumbar injection STZ, after relative female rats lumbar injection STZ, after 4 weeks, blood sugar is just stable, and the level that follow-up study speculates rear estrogen of growing up can produce certain impact to glycolipid metabolism, therefore selects male mouse modeling to subsequent experimental interference less.After the research of Morimoto, studies in China personnel have also carried out the research in this direction in a deep going way, specify that the important function that rat sex plays in N-shaped diabetes rat model further.Therefore combining forefathers' research, present invention design should select male rat as experimental subjects.
1.3 the body weight selection aspect of rat: have researcher to be divided with different weight gradient by experimental rat, then packet carries out type ii diabetes rat model and prepares, result be body weight 160-180g,
181-200g、 201g-220g、221g-240g、 241g-260g、
In six groups of rats of 261g-280g, body weight not only becomes mould rate the highest two groups of rats of 220-240g with 241-260g, and the death rate is extremely low.First this result embodies rat body weight has certain impact to modeling and carrying out of subsequent experimental, next indicates the accuracy in order to increase experiment, in the experimental design procedure of type ii diabetes rat correlative study, the body weight of rat also should be used as one of considerations；This experiment is before modeling, by the regulation nursing cycle, as far as possible by body weight control in 220-240g Yu 241-260g two class range, thus improves modeling success rate, makes experimental result more reliable.The age aspect of rat: forefathers' research relates to rat and the susceptibility of STZ is had age-dependent, the therefore experimental subjects of modeling should select adult rat.Compared to children mouse, adult rat modeling has higher success rate.Therefore this experimental subjects, chooses adult rat.Comprehensive every factor affecting modeling success rate, the experimental rat that present invention experiment is chosen is male adult rat.
Before 1.4 modelings, body weight control is in the range of 220-240g or 241-260g, and STZ injection volume is single intraperitoneal injection 55mg/kg；The regulation effect of the fat disorder that mainly research active material hyperlipidemia concurrent to type ii diabetes is caused due to this experiment, therefore the method that experimental technique uses STZ (induction type ii diabetes)+high lipid food (induced hyperlipidemia), after modeling success, after gastric infusion certain time, using blood fat, blood sugar level change etc. as test index, thus observe whether snow chrysanthemum polysaccharide tea treasure exists regulation effect to rat body weight, blood sugar level and fat disorder.
2, reagent: TC, TG, LDL-C, HDL-C kit is purchased from Nanjing and builds up Bioengineering Research Institute, it is pure that other reagent is domestic analysis.
3, animal experiment method
3.1 animals: cleaning grade male SD rat 40, body weight 90 1 110g.
3.2 feeds: basal feed is that market is common；High lipid food is made up of 90% basal feed, 10% lard, and fresh lard is purchased from supermarket near city.
3.3 animal packet and experimental techniques.
40 rats, after basal feed adapts to feed one week, are taken 8 at random and simply continue to feeding basal feed by body weight, and as normal group (N), " after modeling success, modeling group rat is randomly divided into four groups by body weight to remaining equal feeding high lipid food, often group 8.First group of high lipid food group, HF feeding high lipid food；Second group of high lipid food snow chrysanthemum polysaccharide tea treasure's group, F mono-HF feeding is containing the high lipid food of 5% snow chrysanthemum polysaccharide tea treasure, and i.e. 95% high lipid food+5% snow chrysanthemum polysaccharide tea is precious (low dose group)；3rd group of basal feed group, c feeding basal feed one；4th group of basal feed snow chrysanthemum polysaccharide tea treasure's group, F-C feeding is containing the basal feed of 10% snow chrysanthemum polysaccharide tea treasure, and i.e. 9,0% high lipid food+10% snow chrysanthemum polysaccharide tea is precious (low dose group).
Each treated animal list cage is raised, and indoor temperature controls at 22 1 25 DEG C, and humid control is 40 one 60%, and 12h illumination 12h dark automatically controls, and ad lib is intake, and within every two days, measures body weight and food ration once；After 24 days, each group Rat Fast 24h, after claiming whole body weight, broken end takes blood, separating serum, reference reagent box specification carries out serum total cholesterol (TC), triglycerides (TG), LDL-C (LDL-C), the mensuration of HDL-C (HDL-C) content.Dissect rat, visually observe rat body fat calmness situation and internal organs situation of change, calculate AI.
3.4 statistical procedures: TC, TG, LDL-C, HDL-C are all measured by kit requirement: food utilization=body weight evolution/food ration × 100%；Fat coefficient=fat gross weight/body weight × 100%；AI AI=(TC-HDL-C)/
HDL-C；SPSSl6.0 software is used to carry out one-way analysis of variance.
3.5 result
3.5.1 animal used as test obese model
Before modeling, there was no significant difference for the body weight of two groups of rats, and after modeling, the rat body weight of modeling group is apparently higher than normal rats body weight (P < 0.01), such as table 1.
Table 1: the foundation of obesity rat model
| Group | Rat quantity (only) | Body weight (g) before modeling | Body weight (g) after modeling |
| Normal group | 8 | 90.2±2.4 | 267.8±4.1 |
| Modeling group | 32 | 89.7±2.3 | 289.2±2.54 |
3.5.2 the impact on rat food ration, body weight and weightening finish
As shown in table 2, in experimentation, each group rat shows no obvious abnormalities, and diet activity is in order.Each group rat body weight no significant difference before experiment.At the end of experiment, compared with HF group, F-HF group rat body weight significantly reduces (P < 0.05)；C group, F-C group pole significantly reduce (P < 0.01)；Each group rat all pole of increasing weight significantly reduces (P < 0.01)；The weightening finish of F-HF group, C group, F-C group rat body weight reduces successively.Illustrate that ingesting including snow chrysanthemum polysaccharide tea is precious intervenes body weight and the weightening finish that can significantly reduce nutritive obesity in rats, and it is optimal to combine, with snow chrysanthemum polysaccharide tea treasure, the Adjustment effect that low fat ingests.
F-HF group and HF group are without significant difference, and F-C group and C group, without significant difference, illustrate that avenging the precious food ration to experimental rat of chrysanthemum polysaccharide tea has no significant effect, and it is not to reach the intake of food by minimizing rat that snow chrysanthemum polysaccharide tea treasure reduces the effect of body weight.
Table 2: the precious impact on rat food ration, body weight and weightening finish of snow chrysanthemum polysaccharide tea
3.5.3 on rat fat index and the impact of AI
As shown in table 3, compared with HF group, F-HF group, C group, F-C group rat TC, LDL-C, AI pole significantly reduce (P < 0.01)；HDL-C significantly raises (P < 0.01)；Wherein F-HF group, F-C group rat HDL-C are higher than C group；F-HF group, F-C group rat TG pole significantly reduce (P < 0.01), C group rat TG is without notable change, illustrate to include avenging chrysanthemum polysaccharide tea treasure and diet modification can all significantly reduce TC, LDL-C content in rat body, improve HDL-C content, it is effectively improved blood lipid level in rat body, reduces and suffer from atherosclerotic risk；Snow chrysanthemum polysaccharide tea treasure is better than diet modification to the reduction of TG content in rat body and the elevating effect of HDL-C content.
Table 3: the precious impact on rat blood serum index of snow chrysanthemum polysaccharide tea
Conclusion:As seen from the experiment, the snow chrysanthemum polysaccharide tea using the present invention to prepare is precious the same with diet modification, can significantly reduce the body weight of nutritive obesity in rats, the accumulation of suppression rat body fat, have preferable fat-reducing effect to nutritive obesity in rats；Snow chrysanthemum polysaccharide tea treasure prepared by the present invention can also significantly reduce Serum TC, TG, LDL-C content, significantly improves rat blood serum HDL-C content, has good lipid-lowering effect；The precious reduction to rat blood serum TG content of snow chrysanthemum polysaccharide tea and the elevating effect of HDL-C content prepared by the present invention are especially notable, are better than diet modification.
Result of the test shows: snow chrysanthemum polysaccharide tea prepared by the present invention is precious good functions of lowering blood-fat and reducing weight to nutritive obesity in rats, and this provides a kind of new way for developing natural lowering blood-fat and reducing weight product, but the aspects such as its mechanism of action need to study further.To the snow bioactive further investigation of chrysanthemum, it is possible not only to exploitation snow chrysanthemum and utilizes new way, improve snow chrysanthemum resource utilization, it is also possible to improve snow chrysanthemum producing region economic benefit, there is important social effect.
Embodiment in the present invention described above, is not that the present invention makees other formal restriction, and the technology contents of above-mentioned elaboration can be carried out changing Equivalent embodiments by the personnel of this specialty.As long as but without departing from technical solution of the present invention content, according to the technical spirit of the present invention, simply above example is carried out simple modification, equivalent variations and remodeling, still falls within the protection domain of the technology of the present invention.
Claims (1)
1. a snow chrysanthemum polysaccharide tea is precious, it is characterised in that snow chrysanthemum polysaccharide tea treasure is prepared by method and obtains:
(1) select that neat and well spaced degree is high, bone is full, pistil petal color and luster is orange for flower, the drying snow chrysanthemum of impurity without leaf stalk, pistil smalls or other non-snow chrysanthemum classes, by broken for the snow inulin chosen, the granularity at the snow inulin end after pulverizing is at 100 mesh；
(2) during the ratio by weight 1:30 adds water to the snow inulin end of step (1), stirring with glass bar, preheat half an hour in putting into thermostat water bath, the temperature of thermostat water bath is 55 DEG C；
(3) it is ultrasonically treated that the solution after above-mentioned steps (2) being processed utilizes sonicator to carry out, and temperature time ultrasonically treated is 55 DEG C, time 15min, power 160w；
(4) the snow chrysanthemum solution after above-mentioned steps (3) being processed is poured in enzyme reactor, adds cellulase, pectase and papain in enzyme reactor；The consumption of cellulase is the 1% of snow chrysanthemum powder quality, and the consumption of pectase is the 1% of snow chrysanthemum powder quality, and the consumption of papain is the 1.5% of snow chrysanthemum powder quality, and the time of enzymatic reaction is 3h, temperature 55 DEG C, and pH value is natural；
(5) go out after enzyme reaction terminates ferment treatment by the snow chrysanthemum solution in enzyme reactor, and the temperature of the enzyme that goes out is 100 DEG C, and the time is 5min, makes the enzyme in solution inactivate；
(6) leaching liquor after step (5) being terminated is centrifuged processing, and the centrifugal time is 15min, and rotating speed is 3500r/min, obtains supernatant；
(7) it is spray-dried: in supernatant, add beta-schardinger dextrin, maltodextrin, beta-schardinger dextrin and the mixed quality of maltodextrin and the mass ratio avenging inulin end solid content is 2:3, be spray-dried；Beta-schardinger dextrin is formed by quality 1:1 with maltodextrin；EAT 170～180 DEG C, temperature of outgoing air 78～87 DEG C, dried material moisture≤5%；
(8) the snow chrysanthemum polysaccharide tea treasure after being spray-dried carries out packaging and obtains snow chrysanthemum polysaccharide tea treasure's finished product.
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| CN104586910B (en) * | 2014-12-10 | 2018-05-25 | 新疆华铁能源科技有限公司 | Coreopsis tinctoria extract and preparation method thereof |
| CN104872333A (en) * | 2015-05-28 | 2015-09-02 | 浙江农林大学 | Manufacture method of cymose buckwheat rhizome seed tea powder |
| CN104996652A (en) * | 2015-06-01 | 2015-10-28 | 新疆生命核力高科股份有限公司 | Sanvitalia procumbens beverage and processing method thereof |
| CN107173490A (en) * | 2017-05-16 | 2017-09-19 | 吴启川 | A kind of dog bull brain tea beverage processing technology |
| CN107319057A (en) * | 2017-08-26 | 2017-11-07 | 安徽阜南常晖食品有限公司 | A kind of preparation method of stem of noble dendrobium green tea beverage |
| CN109846964A (en) * | 2019-04-16 | 2019-06-07 | 滨州医学院附属医院 | Using chrysanthemum as anti-inflammatory tea-drinking of primary raw material and preparation method thereof |
| CN115886088A (en) * | 2022-11-22 | 2023-04-04 | 深圳市深宝华城科技有限公司 | Method for extracting tea leaves by combining repeated freezing and thawing with dynamic ultrahigh-pressure micro-jet technology |
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| CN102696811B (en) * | 2011-12-02 | 2014-04-16 | 湖南梅山黑茶有限公司 | Preparation method of instant black tea |
| CN102599309B (en) * | 2012-03-23 | 2014-08-06 | 新疆科宇科技有限公司 | Coreopsis tinctoria extract and coreopsis tinctoria health instant tea and production method thereof |
| CN102696834A (en) * | 2012-06-21 | 2012-10-03 | 唐军涛 | Instant senecio cineraria tea cream and preparing process thereof |
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