CN103947643B - Special root-growing agent of a kind of Vitex rotundifolia and preparation method thereof and using method - Google Patents

Special root-growing agent of a kind of Vitex rotundifolia and preparation method thereof and using method Download PDF

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Publication number
CN103947643B
CN103947643B CN201410169327.1A CN201410169327A CN103947643B CN 103947643 B CN103947643 B CN 103947643B CN 201410169327 A CN201410169327 A CN 201410169327A CN 103947643 B CN103947643 B CN 103947643B
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vitex rotundifolia
root
growing agent
cuttings
vitex
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CN201410169327.1A
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CN103947643A (en
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黄东风
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Institute of Soil and Fertilizer Fujian Academy of Agricultural Sciences
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Institute of Soil and Fertilizer Fujian Academy of Agricultural Sciences
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Abstract

The invention discloses special root-growing agent of a kind of Vitex rotundifolia and preparation method thereof and using method, containing heteroauxin 50mg, methyl α-naphthyl acetate 250mg in the special root-growing agent of 1000ml Vitex rotundifolia, surplus is deionized water.By the IAA solution of 100mg/L and the NAA solution of 500mg/L, by equal-volume mixing, be the special root-growing agent of Vitex rotundifolia.Be immersed in by Vitex rotundifolia spray cuttings base portion in the special root-growing agent solution of Vitex rotundifolia, soaking depth 6 ~ 8cm, soak time 1h, namely the Vitex rotundifolia cuttings of taking out after soaking is applied to cuttings plantation and breeds.The special root-growing agent of Vitex rotundifolia of the present invention, than contrasting or using common root-growing agent to significantly improve the survival rate of Vitex rotundifolia cuttings, digit rate of taking root and number of taking root, shorten the initial rootage duration of cuttings, promote cuttings early growth and quick tiller, be worth wider further genralrlization application in production.

Description

Special root-growing agent of a kind of Vitex rotundifolia and preparation method thereof and using method
Technical field
The invention belongs to agriculture field, be specifically related to special root-growing agent of a kind of Vitex rotundifolia and preparation method thereof and using method.
Background technology
Medicinal fructus viticis be Verbenaceae Vitex rotundifolia ( vitex trifolia L.var.simplicifolia Cham) and wild pepper ( vitex trifolia L) dry mature fruit, be the legal parts of generic medicinal plants that " Chinese Pharmacopoeia " records, Vitex rotundifolia row top grade.Vitex rotundifolia is perennial liana shrub plant, primary growth in sandy beach, beach, river bank, inland sand ground etc.Fructus viticis determined curative effect, medicinal history is long, and begin to be loaded in the Shennong's Herbal of the Eastern Han Dynasty, is specified in " Tang materia medica " of the Tang Dynasty, and recent studies achievement is loaded among modern medical science monumental work " China's book on Chinese herbal medicine ".Fructus viticis is cool in nature, bitter is pungent, effect of tool dispelling wind and heat from the body, the clear sharp head; Cure mainly affection of exogenous wind-heat, dizzy headache, antimigraine, swelling and aching of gum, red eye, swell pain, many tear, or pain in order eyeball, dim not clear, the diseases such as arthritis with fixed pain caused by dampness contraction.Vitex rotundifolia, containing effective ingredients such as Alkaloid, vitamin A, negundo chastetree fruit flavones, has high medical value.Modern pharmacological research shows: fructus viticis have anti-microbial effect, analgesic activity, antiinflammatory action, hypotensive activity, anticoagulation, eliminating phlegm and relieving asthma effect, to effects of curing the disease widely such as intestinal tube smooth muscle effects.So in Chinese Medicinal Materials Markets the fructus viticis of high-quality supply falls short of demand.Due to the huge market demand, gathering excessively, adding that sand beach people is serious for digging husky destruction; Vitex rotundifolia resource is caused to reduce rapidly; the continuous exhaustion of germ plasm resource is degenerated, and belongs to endangered plants, has been listed in Key Protected medicinal material species register (three classes).
The propagation method of current Vitex rotundifolia mainly contains seminal propagation, offshoot and cuttage etc. 3 kinds.Wherein, seminal propagation mode, not only time-consumingly takes a lot of work, and first will use the after-ripening of damp sand lamination, broadcast front warm water pretreatment, and can emerge through 30 ~ 40 days, and emergence rate be low, just can transplant after 2 ~ 3 years, and at least needing more than 5 years could large volume productions fruit.Propagation by division coefficient is little, should not large area plant.And cottage propagation is not only simple and easy to do, produce effects soon, effective, and adopt kind of bar vegetative propagation technique to keep offspring's breeding characteristic more reliable and more stable than seed sexual propagation, therefore production above adopts cuttage more.But the survival rate of at present Vitex rotundifolia cuttings cuttage is general not high, take root slow and rooting rate is not high, be to hinder Vitex rotundifolia large area to expand one of numerous principal element.
Summary of the invention
The object of the present invention is to provide and a kind ofly can improve special root-growing agent of Vitex rotundifolia of Vitex rotundifolia cuttage cuttings survival rate and preparation method thereof and using method.
For achieving the above object, the present invention adopts following technical scheme:
The present invention provide firstly the special root-growing agent of a kind of Vitex rotundifolia, and it is the solution be made up of the raw material of following proportioning:
Containing heteroauxin 50 mg, methyl α-naphthyl acetate 250 mg in 1000ml solution, surplus is deionized water.
Preparation method is as follows:
1) preparation of initial stoste 1: heteroauxin deionized water dissolving, is mixed with the IAA solution that mass concentration is 100 mg/L;
2) preparation of initial stoste 2: methyl α-naphthyl acetate deionized water dissolving, is mixed with the NAA solution that mass concentration is 500 mg/L;
3) preparation of the special root-growing agent of Vitex rotundifolia: by step 1) and 2) the initial stoste 1 prepared respectively and initial stoste 2, by equal-volume mixing, be the special root-growing agent of Vitex rotundifolia.
Be immersed in by Vitex rotundifolia spray cuttings base portion in the special root-growing agent solution of Vitex rotundifolia, soaking depth 6 ~ 8 cm, soak time 1 h, namely the Vitex rotundifolia cuttings of taking out after soaking is applied to cuttings plantation and breeds.
Described Vitex rotundifolia spray, chooses stalwartness, gives birth to Vitex rotundifolia branch without l ~ 2 year of damage by disease and insect, be cut into 20 cm long, the cuttings of tool 2 ~ 3 joint.
Heteroauxin, i.e. indole-3-acetic acid, Indole-3-acetic acid, is abbreviated as IAA; Methyl α-naphthyl acetate, i.e. 2-(1-naphthyl) acetic acid, English general 1-naphthylacetic acid by name, be abbreviated as NAA, other names are called α-naphthaleneacetic acid.
Remarkable advantage of the present invention is:
(1) the special root-growing agent of Vitex rotundifolia of the present invention, comparable contrast (clear water immersion) or use common root-growing agent (ABT root-inducing powder) to significantly improve the survival rate of Vitex rotundifolia cuttings, digit rate of taking root and number of taking root, shorten the initial rootage duration of cuttings, promote cuttings early growth and quick tiller, be worth wider further genralrlization application in production.
(2) prepare the general reagent that the present invention's heteroauxin used and methyl α-naphthyl acetate are all lower, the easy purchases of price, and manufacturing process is simple to operate, easily-learned easily mastered.
Embodiment
The special root-growing agent of a kind of Vitex rotundifolia, its concrete preparation method's step is as follows:
1) preparation of initial stoste 1: take heteroauxin in plastic basin, add the abundant agitating solution of deionized water, added heteroauxin is dissolved completely, be mixed with the IAA solution that mass concentration is 100 mg/L.
2) preparation of initial stoste 2: take methyl α-naphthyl acetate in plastic basin, add the abundant agitating solution of deionized water, added methyl α-naphthyl acetate is dissolved completely, be namely mixed with the NAA solution that mass concentration is 500 mg/L.
3) preparation of the special root-growing agent of Vitex rotundifolia: by step 1) the initial stoste 1 prepared, with step 2) initial stoste 2 equal-volume prepared mixes, abundant stirring and evenly mixing, is the special root-growing agent being applicable to promote Vitex rotundifolia cuttage cuttage rooting prepared.
Choose stalwartness, give birth to Vitex rotundifolia branch without l ~ 2 year of damage by disease and insect, be cut into 20 cm long, the cuttings of tool 2 ~ 3 joint, Vitex rotundifolia cuttage cuttings base portion 6 ~ 8 cm is immersed in step 3) 1 h in the special root-growing agent solution that is prepared into, takes out the Vitex rotundifolia cuttings after soaking, inserts seedbed by seeding row spacing 6 cm × 15 cm, bury and be deeply about 2/3 of cuttings, compacting earth, waters permeable, covers grass.Cuttage person in spring field planting in autumn then, Autumn Cutting person transplants next early April in spring.
Concrete implementation result
In order to verify the take root facilitation effect of special root-growing agent of the present invention to Vitex rotundifolia cuttage cuttings, adopt husky bed as seedling medium, asexual branch cutting nursery.Elite selecting uses more general ABT root-inducing powder to be reference in the market, design 5 experimental group, that is: (1) contrast (clear water immersion), (2) ABT root-growing agent (500 mg/L), (3) special root-growing agent formula 1, i.e. IAA (50mg/L)+NAA (400mg/L) (the same the present invention of preparation method), (4) special root-growing agent formula 2, the i.e. special root-growing agent of Vitex rotundifolia of the present invention, IAA (100mg/L)+NAA (500mg/L), (5) special root-growing agent formula 3, i.e. IAA (150mg/L)+NAA (600mg/L) (the same the present invention of preparation method).Each experimental group establishes 6 repetitions.Vitex rotundifolia nursery base, Putian City Xiuyu District is picked up from for examination cuttage Vitex rotundifolia branch, wild pepper cuttings specification is long 20 cm, husky bed specification is 150cm (length) × 90 cm (wide) × 18cm (height), the strain of each husky bed cuttage wild pepper branch 150, before cuttage kind bar, first soak cuttings base portion 6 ~ 8 cm 1 h by the different root-growing agents (or equivalent clear water) of test Treatment Design.Test was from March 10, and after cuttage, 30d and 45 d gets the situation of taking root of each process 3 repeated observation cuttage branches, and calculates the digit rate of taking root of cuttage branch.Duration of test every day respectively to spray husky bed with 5000ml clear water for 2 times sooner or later, and maintenance matrix is moistening, is beneficial to the normal growth of cuttage branch.
After cuttage, the result of the test (see table 1) of 30 d shows: compared with processing with contrast (clear water soaks), process with Vitex rotundifolia " special root-growing agent formula 1 ~ 3 " and " ABT root-growing agent " and all can significantly improve the survival rate of Vitex rotundifolia cuttings, digit rate of taking root and individual plant and to take root number, increase rate is respectively 37.59% ~ 50.09%, 37.59% ~ 52.53%, 31.62 ~ 58.97%; Wherein, " special root-growing agent formula 2 " is better than " ABT root-inducing powder " and other 2 groups special root-growing agent formulas generally to the facilitation effect of Vitex rotundifolia cuttage rooting, and the individual plant especially significantly improving cuttings is taken root number.
(after cuttage, 30 d) on the impact of wild pepper cutting survival rate and situation of taking root for the different plant establishment agent of table 1
Note: digit rate of taking root (%)=(kind of a number of taking root/cuttage kind number) × 100; Different lowercases after same column numerical value represent significant difference (p<0.05).
After cuttage, the result of the test (see table 2) of 45 d shows: compared with processing with contrast (clear water soaks), process with Vitex rotundifolia " special root-growing agent formula 1 ~ 3 " and " ABT root-growing agent " and all can significantly improve the survival rate of Vitex rotundifolia cuttings, digit rate of taking root and individual plant further and to take root number, increase rate is respectively 43.2% ~ 61.01%, 43.37% ~ 57.77%, 52.38 ~ 95.24%; Wherein, " special root-growing agent formula 2 " is obviously better than " ABT root-growing agent " and other 2 groups special root-growing agent formulas to the facilitation effect of Vitex rotundifolia cuttage rooting, improves survival rate 10.58 % of Vitex rotundifolia cuttings, digit rate of taking root 9.58% and individual plant take root several 26.80% than use " ABT root-growing agent ".
(after cuttage, 45 d) on the impact of wild pepper cutting survival rate and situation of taking root for the different plant establishment agent of table 2

Claims (2)

1. a using method for the special root-growing agent of Vitex rotundifolia, is characterized in that: the special root-growing agent of described Vitex rotundifolia is the solution be made up of the raw material of following proportioning:
Containing heteroauxin 50 mg, methyl α-naphthyl acetate 250 mg in 1000ml solution, surplus is deionized water; Be immersed in by Vitex rotundifolia spray cuttings base portion in the special root-growing agent solution of Vitex rotundifolia, soaking depth 6 ~ 8 cm, soak time 1 h, namely the Vitex rotundifolia cuttings of taking out after soaking is applied to cuttings plantation and breeds.
2. the using method of the special root-growing agent of Vitex rotundifolia according to claim 1, is characterized in that: described Vitex rotundifolia spray, chooses stalwartness, gives birth to Vitex rotundifolia branch without l ~ 2 year of damage by disease and insect, be cut into 20 cm long, the cuttings of tool 2 ~ 3 joint.
CN201410169327.1A 2014-04-25 2014-04-25 Special root-growing agent of a kind of Vitex rotundifolia and preparation method thereof and using method Expired - Fee Related CN103947643B (en)

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CN104429845B (en) * 2014-09-25 2016-04-20 阜南县永兴工艺品有限公司 A kind of high-efficiency grow method of the twigs of the chaste tree
CN104855119A (en) * 2015-06-09 2015-08-26 黄振忠 Fructus viticis cutting seedling method
CN111357556A (en) * 2020-04-17 2020-07-03 山东胜伟盐碱地科技有限公司 Method for breeding new variety of vitex rotundifolia on saline-alkali soil

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