CN103923850A - Microbial preparation for degrading nitrites and inhibiting blue algae and its preparation method - Google Patents

Abstract

The invention relates to a microbial preparation for degrading nitrites and inhibiting blue algae and its preparation method; after submerged fermentation of bacillus subtilis with the nitrite degradation function and lactobacillus acidophilus strains with blue algae inhibiting function in separate liquids, dry powder is prepared, and the dry powder is proportionally mixed to prepare the microbial preparation; the new microbial preparation with nitrite degradation and blue algae inhibiting dual effects is provided.

Description

Microbial preparation of a kind of degrading nitrite and inhibition blue-green algae and preparation method thereof

Technical field

The invention belongs to agricultural application microbial technology field.

Background technology

China's culture fishery is mainly taking high-density breeding as main at present, therefore raiser is faced with and continues to increase on the one hand feed injected volume in order to pursue high yield, affects the contradiction of fishes and shrimps Individual Size and output on the one hand because a large amount of residual baits, the discharge of cultivated animals ight soil too much cause the ammonia nitrogen of water body and nitrite to raise.This contradiction is seriously restricting the Sustainable development of China's water industry.Microbial preparation product function on market is single now, cannot meet high-density breeding, so it is multi-functional to be badly in need of developing one, meets the microbial preparation of the environmentally safe of high-density breeding.

Summary of the invention

Technical problem

The object of this invention is to provide " microbial preparation of a kind of degrading nitrite and inhibition blue-green algae and preparation method thereof ", to there is the subtilis of degrading nitrite function and have after the independent liquid submerged fermentation of lactobacillus acidophilus strains that suppresses blue-green algae function, make after dry powder is mixed in proportion and make preparation, for aquaculture provides a kind of degrading nitrite and suppresses the novel microorganism preparation of blue-green algae.

Technical scheme

Microbial preparation of a kind of degrading nitrite of the present invention and inhibition blue-green algae and preparation method thereof, its step is as follows:

(1) subtilis having activated is inoculated in substratum and carries out shaking table cultivation by the inoculum size of 5%-10%, this substratum is: sucrose 15-20g/l, peptone 3-5g/l, sodium-chlor 5-10g/l, PH=7.0-7.2.Culture condition is: 150-200r/min, temperature are that 35-37 degree, air flow are 1: 1; Cultivate after 24-48 hour after concentrated, dry and make subtilis viable bacteria pulvis;

(2) the Lactobacterium acidophilum bacterial classification having activated is inoculated into and in substratum, carries out shaking table cultivation by the inoculum size of 5%-10%, this substratum is as follows: molasses 20-30g/l, yeast extract paste 4-8g/l, potassium primary phosphate 5-10g/l, ammonium sulfate 10-20g/l, magnesium sulfate 0.5-2g/l, with 121 degree sterilizing after adjusting PH=6-8 after tap water dissolving 20 minutes; Culture condition is: culture temperature is that 30-37 degree leaves standstill concentrated after 36-72 hour, the dry live lactobacillus acidophilus pulvis of making of cultivation;

(3) by above-mentioned subtilis viable bacteria pulvis, live lactobacillus acidophilus pulvis and zeolite powder in (1-3): (1-3): ratio (1-3) is mixed the composite microbial preparation that obtains double effects.

Embodiment:

Embodiment 1:

The subtilis having activated is inoculated in substratum and carries out shaking table cultivation by 5% inoculum size, and this substratum is: sucrose 20g/l, peptone 5g/l, sodium-chlor 5g/l, PH=7.0.Culture condition is: 180r/min, temperature are that 35 degree, air flow are 1: 1; Cultivate after 26 hours after concentrated, dry and make bacillus subtilis bacteria powder;

The Lactobacterium acidophilum bacterial classification having activated is inoculated into and in substratum, carries out shaking table cultivation by 5% inoculum size, this substratum is as follows: molasses 20g/l, yeast extract paste 4g/l, potassium primary phosphate 5g/l, ammonium sulfate 10g/l, magnesium sulfate 0.5g/l, with 121 degree sterilizing after adjusting PH=6-8 after tap water dissolving 20 minutes; Culture condition is: culture temperature is 35 degree, and standing incubation time is concentrated after 72 hours, the dry live lactobacillus acidophilus pulvis of making;

Above-mentioned bacillus subtilis bacteria powder, Lactobacterium acidophilum pulvis and zeolite powder are mixed to the composite microbial preparation that obtains double effects in the ratio of 1: 1: 1.

Embodiment 2:

The subtilis having activated is inoculated in substratum and carries out shaking table cultivation by 5% inoculum size, and this substratum is: sucrose 15g/l, peptone 6g/l, sodium-chlor 6g/l, PH=7.2.Culture condition is: 160r/min, temperature are that 36 degree, air flow are 1: 1; Cultivate after 32 hours after concentrated, dry and make bacillus subtilis bacteria powder;

The Lactobacterium acidophilum bacterial classification having activated is inoculated into and in substratum, carries out shaking table cultivation by 5% inoculum size, this substratum is as follows: molasses 25g/l, yeast extract paste 6g/l, potassium primary phosphate 6g/l, ammonium sulfate 15g/l, magnesium sulfate 0.8g/l, with 121 degree sterilizing after adjusting PH=6 after tap water dissolving 20 minutes; Culture condition is: culture temperature is 30 degree, and incubation time is concentrated after 60 hours, the dry Lactobacterium acidophilum pulvis of making;

Above-mentioned bacillus subtilis bacteria powder, Lactobacterium acidophilum pulvis and zeolite powder are mixed to the composite microbial preparation that obtains double effects in the ratio of 2: 1: 1.

Claims (2)

1. a microbial preparation for degrading nitrite and inhibition blue-green algae, is characterized in that: be made up of with the live lactobacillus acidophilus with inhibition blue-green algae function the subtilis viable bacteria with degrading nitrite.

2. a preparation method for the microbial preparation of degrading nitrite and inhibition blue-green algae, is characterized in that preparing as follows:

(1) subtilis having activated is inoculated in substratum and carries out shaking table cultivation by the inoculum size of 5%-10%, this substratum is: sucrose 15-20g/l, peptone 3-5g/l, sodium-chlor 5-10g/l, PH=7.0-7.2.Culture condition is: 150-200r/min, temperature are that 35-37 degree, air flow are 1: 1; Cultivate after 24-48 hour after concentrated, dry and make subtilis viable bacteria pulvis;

(2) the Lactobacterium acidophilum bacterial classification having activated is inoculated into and in substratum, carries out shaking table cultivation by the inoculum size of 5%-10%, this substratum is as follows: molasses 20-30g/l, yeast extract paste 4-8g/l, potassium primary phosphate 5-10g/l, ammonium sulfate 10-20g/l, magnesium sulfate 0.5-2g/l, with 121 degree sterilizing after adjusting PH=6-8 after tap water dissolving 20 minutes; Culture condition is: culture temperature is that 30-37 degree leaves standstill concentrated after 36-72 hour, the dry live lactobacillus acidophilus pulvis of making of cultivation;

(3) by above-mentioned subtilis viable bacteria pulvis, live lactobacillus acidophilus pulvis and zeolite powder in (1-3): (1-3): ratio (1-3) is mixed the composite microbial preparation that obtains double effects.