CN102250791A - Method for producing bacillus subtilis microbial inoculum by taking soybean cake meal as major raw material - Google Patents
Method for producing bacillus subtilis microbial inoculum by taking soybean cake meal as major raw material Download PDFInfo
- Publication number
- CN102250791A CN102250791A CN2011101614030A CN201110161403A CN102250791A CN 102250791 A CN102250791 A CN 102250791A CN 2011101614030 A CN2011101614030 A CN 2011101614030A CN 201110161403 A CN201110161403 A CN 201110161403A CN 102250791 A CN102250791 A CN 102250791A
- Authority
- CN
- China
- Prior art keywords
- soybean cake
- bacillus subtilis
- culture medium
- raw material
- glucose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000068988 Glycine max Species 0.000 title claims abstract description 18
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 18
- 244000063299 Bacillus subtilis Species 0.000 title claims abstract description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 11
- 239000002994 raw material Substances 0.000 title claims abstract description 10
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 9
- 235000012054 meals Nutrition 0.000 title abstract 3
- 239000002068 microbial inoculum Substances 0.000 title abstract 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims abstract description 22
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 12
- 239000008103 glucose Substances 0.000 claims abstract description 12
- 235000019270 ammonium chloride Nutrition 0.000 claims abstract description 11
- 239000001963 growth medium Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 230000000813 microbial effect Effects 0.000 claims description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 abstract description 18
- 230000004151 fermentation Effects 0.000 abstract description 18
- 241001052560 Thallis Species 0.000 abstract description 5
- 244000005700 microbiome Species 0.000 abstract description 2
- 230000003115 biocidal effect Effects 0.000 description 5
- 239000006041 probiotic Substances 0.000 description 5
- 235000018291 probiotics Nutrition 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- 108010062877 Bacteriocins Proteins 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of industrial microorganisms and fermentation engineering, and particularly relates to a fermentation culture medium for producing a bacillus subtilis microbial inoculum by taking soybean cake meal as a major raw material. The culture medium comprises the following components in percentage by weight: 2-4 percent of soybean cake meal, 0.1-0.5 percent of glucose, 0.05-0.15 percent of ammonium chloride and the balance of water. By adopting the culture medium, mass propagation of bacillus subtilis at a growing period can be facilitated, thalli are kept robust and regular, and the production cost is reduced under the condition of keeping the product quality invariant.
Description
Technical field
The invention belongs to industrial microorganism fermentation engineering field, relate in particular to a kind of production method of bacillus subtilis microbial agent.
Background technology
In recent years, the aquaculture develop rapidly, the a large amount of uses in the intensification large-scale production process of microbiotic and other chemicals, though reached volume increase and disease-resistant purpose, but antibiotic long-term abuse has not only caused the resistance of pathogenic bacteria, and antibiotic remains is directly threatening human beings'health.Antibiotic remains has become the international trade barrier of the multiple cultured product of China, makes Enterprises for Export in China suffer enormous economic loss.Country takes severe means in recent years, and strictness limits microbiotic or forbids some antibiotic use, and actively seeks its substitute.
The development of microbiotic substitute (as probiotics, oligosaccharides, antibacterial peptide etc.) becomes various countries scholar's research focus.Probiotics is brought into use very early, developed rapidly all over the world since the twentieth century, to the beginning of the eighties existing multiple little ecological goods put on market, and quantity and kind constantly enlarge.The probiotics for animals of the bacillus category that contemporaneity China succeeds in developing, lactic acid bacteria class and budding fungus is widely used as fodder additives.
Subtilis is a kind of aerobic bacteria, except producing proteolytic enzyme, lipase, amylase etc., can also secrete antibiotic, be that China Ministry of Agriculture allows one of two kinds of genus bacillus that can be used as fodder-grade micro-ecological additive, and it is developed into feeding micro-ecological preparation more and more.In addition; subtilis more is used in fields such as livestock industry, medicine industry, control of plant disease and environment protection widely as a kind of important industrial strain; so have development prospect widely, demonstrate huge social benefit and ecological benefits.
At present, producing bacillus subtilis microbial agent is main raw material with food crop such as starch, Semen Maydis powder all, and process is produced probiotics by fermentation.In patent: in the purposes (CN200510061554.3) of a kind of lichem bacillus strain and the bacteriocin that produced thereof, the substratum of producing bacteriocin is the LB substratum, and its main component is expensive peptone and yeast extract.But, in recent years,, cause the leavened prod cost increase because the food crop price constantly rises.The present application people found through experiments the agricultural byproducts soybean cake powder has the growth of promotion effect to various bacillus and the mould that produces probiotics, as raw materials for production, can significantly reduce the consumption of grains such as starch, glucose, Semen Maydis powder, yeast extract/yeast extract, peptone, its production cost is reduced.
Summary of the invention
At the too high weak point of raw materials cost in the above technology, the objective of the invention is to adopt cheap soybean cake powder is that main fermentation raw material is produced bacillus subtilis microbial agent, make present method not only keep the advantage of above-mentioned technology, and production cost is descended significantly.
A kind of soybean cake powder that adopts is the method that main fermentation raw material is produced bacillus subtilis microbial agent, it is characterized in that fermenting process may further comprise the steps:
1, be that main raw material is made culture medium solution with the soybean cake powder;
2, Bacillus subtilis strain is inserted in the said culture medium solution cultivate; It is characterized in that also comprising in the said culture medium solution glucose and ammonium chloride.
The technical solution adopted in the present invention is: it is that main raw material adds an amount of glucose and ammonium chloride that traditional fermention medium (LB substratum) is changed to the soybean cake powder, repeatedly batch fermentation experimental results show that, viable count average out to 3.68 hundred million cfu/ml in this fermented liquid, be more or less the same with viable count mean number 3.8 hundred million cfu/ml in the LB substratum, and the thalli growth state consistency.
The weight percentage of each component is respectively in the substratum of the present invention:
Soybean cake powder 2-4%
Glucose 0.1-0.5%
Ammonium chloride 0.05-0.15%
Surplus is a water
Experiment showed, fermention medium (that is: soybean cake powder 2-4%; Glucose 0.1-0.5%; Ammonium chloride 0.05-0.15%; Surplus is a water) in the change of each component little to fermentation level influence, and thalline is sturdy neat, makes the fermentation level of 5L fermentor tank reach the level of average 3.75 hundred million cfu/ml, under the constant situation of quality product, greatly reduces production cost.
Embodiment
Embodiment one
Adopt (by weight) substratum of following proportioning:
Soybean cake powder 2%
Glucose 0.1%
Ammonium chloride 0.05%
Surplus is a water
Culture condition: temperature is 28 ℃, and pH is 7.0-7.5, and stirring velocity is 150-180rpm, air flow is 2L/min, fermentation period is 36 hours, in the fermentation cylinder for fermentation of 5L, adopts the plate dilution assay method viable count, triplicate is averaged, viable count can reach 3.68 hundred million cfu/ml, and the viable count of LB substratum is 3.75 hundred million cfu/ml, and the thalli growth state consistency, size evenly, and is sturdy neat.
Embodiment two
Adopt (by weight) substratum of following proportioning:
Soybean cake powder 4%
Glucose 0.5%
Ammonium chloride 0.15%
Surplus is a water
Culture condition: temperature is 28 ℃, and pH is 7.0-7.5, and stirring velocity is 150-180rpm, air flow is 2L/min, fermentation period is 36 hours, in the fermentation cylinder for fermentation of 5L, adopts the plate dilution assay method viable count, triplicate is averaged, viable count can reach 3.7 hundred million cfu/ml, and the viable count of LB substratum is 3.8 hundred million cfu/ml, and the thalli growth state consistency, size evenly, and is sturdy neat.
Embodiment three
Adopt (by weight) substratum of following proportioning:
Soybean cake powder 3%
Glucose 0.3%
Ammonium chloride 0.1%
Surplus is a water
Culture condition: temperature is 28 ℃, and pH is 7.0-7.5, and stirring velocity is 150-180rpm, air flow is 2L/min, fermentation period is 36 hours, in the fermentation cylinder for fermentation of 5L, adopts the plate dilution assay method viable count, triplicate is averaged, viable count can reach 3.75 hundred million cfu/ml, and the viable count of LB substratum is 3.82 hundred million cfu/ml, and the thalli growth state consistency, size evenly, and is sturdy neat.
Claims (3)
1. one kind is the method for main material production bacillus subtilis microbial agent with the soybean cake powder, it is characterized in that may further comprise the steps:
1) be that main raw material is made culture medium solution with the soybean cake powder;
2) Bacillus subtilis strain is inserted in the described culture medium solution cultivate, it is characterized in that needing simultaneously in the described culture medium solution to add glucose and ammonium chloride.
2. substratum as claimed in claim 1 is characterized in that, by weight percentage, the weight percentage of each component is in this substratum: soybean cake powder 2-4%; Glucose 0.1-0.5%; Ammonium chloride 0.05-0.15%; Surplus is a water.
3. substratum as claimed in claim 1 is characterized in that, by weight percentage, and the content of soybean cake powder preferably 3%; The content of glucose best 0.3%; The content of ammonium chloride best 0.1%; Surplus is a water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101614030A CN102250791A (en) | 2011-05-28 | 2011-05-28 | Method for producing bacillus subtilis microbial inoculum by taking soybean cake meal as major raw material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101614030A CN102250791A (en) | 2011-05-28 | 2011-05-28 | Method for producing bacillus subtilis microbial inoculum by taking soybean cake meal as major raw material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102250791A true CN102250791A (en) | 2011-11-23 |
Family
ID=44978363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101614030A Pending CN102250791A (en) | 2011-05-28 | 2011-05-28 | Method for producing bacillus subtilis microbial inoculum by taking soybean cake meal as major raw material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102250791A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102604871A (en) * | 2012-03-27 | 2012-07-25 | 福建省农业科学院土壤肥料研究所 | Probiotics fungicide for fermenting bean pulp and preparation method thereof |
| CN103194411A (en) * | 2013-04-08 | 2013-07-10 | 中国农业大学 | Bacillus subtilis and application of bacillus subtilis to production of antimicrobial peptide |
| CN104388367A (en) * | 2014-12-19 | 2015-03-04 | 东北农业大学 | Fermentation medium for phthalic acid dibutyl degrading bacteria |
-
2011
- 2011-05-28 CN CN2011101614030A patent/CN102250791A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102604871A (en) * | 2012-03-27 | 2012-07-25 | 福建省农业科学院土壤肥料研究所 | Probiotics fungicide for fermenting bean pulp and preparation method thereof |
| CN103194411A (en) * | 2013-04-08 | 2013-07-10 | 中国农业大学 | Bacillus subtilis and application of bacillus subtilis to production of antimicrobial peptide |
| CN103194411B (en) * | 2013-04-08 | 2015-04-15 | 中农颖泰林州生物科园有限公司 | Bacillus subtilis and application of bacillus subtilis to production of antimicrobial peptide |
| CN104388367A (en) * | 2014-12-19 | 2015-03-04 | 东北农业大学 | Fermentation medium for phthalic acid dibutyl degrading bacteria |
| CN104388367B (en) * | 2014-12-19 | 2018-07-13 | 东北农业大学 | A kind of fermentation medium of dibutyl phthalate degradation bacterium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101698539B (en) | Microecological agent for purifying water and preparation method thereof | |
| CN102696865B (en) | Method for producing probiotic bacteria granular feed by fermenting corn straw with mixed strain | |
| CN105670976B (en) | Fermentation medium of bacillus amyloliquefaciens and application thereof | |
| CN104082345A (en) | Special EM (Effective Microorganisms) bacteria powder for aquaculture and preparation method and application of special EM (Effective Microorganisms) bacteria powder | |
| CN102583776A (en) | Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method | |
| CN103468594B (en) | Candidautilis strain and application thereof | |
| CN102919516A (en) | Novel vinasse biological feed and production process thereof | |
| CN102719380B (en) | Aquatic compound microecologics and preparation method thereof | |
| CN103484421B (en) | A kind of chlamydosporic method of liquid fermenting scale up test Gliocladium roseum | |
| CN105432935A (en) | Production method for functional amino-acid humic-acid microecological preparation for aquatic animal and poultry | |
| CN103964908A (en) | Compound microorganism agent for compound microorganism fermentation of organic solid wastes | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN103749967A (en) | Sweet potato residue fermentation feed and preparation method thereof | |
| CN101884394B (en) | Composite microbial feed additive for chicken and preparation method thereof | |
| CN107603916A (en) | A kind of antimicrobial composition microbial inoculum, its preparation method and application | |
| CN101973806A (en) | Nanocrystallization compound-type biological liquid fertilizer and production method thereof | |
| CN108085265A (en) | Bacillus coagulans new strains and its probiotics and feed | |
| CN1653936B (en) | Method for preparing composite micro-ecological enzyme for feeding the ruminant by using disused mushroom dregs | |
| CN104388341A (en) | Bacillus mucilaginosus strain and application thereof in seaweed degradation | |
| CN102250791A (en) | Method for producing bacillus subtilis microbial inoculum by taking soybean cake meal as major raw material | |
| CN103719537A (en) | Nonreactive biological fermented feed and preparation method thereof | |
| CN109055264A (en) | A kind of microbial bacterial agent and preparation method thereof for holothruian cultures | |
| CN103829040B (en) | A kind of with the method for Chen Xiangcha and bacillus subtilis composite fermentation dregs of beans and application | |
| CN102787153B (en) | Method for producing enramycin by microbial fermentation supplement feed | |
| CN108587966B (en) | Pure natural plant source EM (effective microorganisms) culture medium and application of fermentation product thereof in aquaculture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20111123 |