CN101803679B - Micro-ecological feed additive for enhancing color of pet fish and preparation method thereof - Google Patents
Micro-ecological feed additive for enhancing color of pet fish and preparation method thereof Download PDFInfo
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- CN101803679B CN101803679B CN2009102489172A CN200910248917A CN101803679B CN 101803679 B CN101803679 B CN 101803679B CN 2009102489172 A CN2009102489172 A CN 2009102489172A CN 200910248917 A CN200910248917 A CN 200910248917A CN 101803679 B CN101803679 B CN 101803679B
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Abstract
The invention relates to fish culture, in particular to a micro-ecological feed additive for enhancing the color of pet fish and a preparation method thereof. The micro-ecological feed additive of the invention comprises the following components in parts by weight: 1-3 parts of candida utilis, 1-3 parts of enterococcus faecalis, 1-3 parts of lactobacillus acidophilus and 1-3 parts of bacillus subtilis; and the components are cultured in a culture medium until the pH value is 2.5-3.5 to obtain the feed additive. The product of the invention has rich nutrients and is green and environment friendly, and has good effects on the adjusting and control of the water quality in the process of culturing the pet fish and the healthy growth of the pet fish.
Description
Technical field
The present invention relates to fishery cultivating, specifically microbial forage additive of a kind of enhancing color of pet fish and preparation method thereof.
Background technology
During reality was produced, it was formulated by materials such as fish meal, dregs of beans, spirulina powder and VITAMINss mostly that many feed stuff for pet fish are arranged, and these feeds have the growth of promotion to fishing gear; Improve immunizing power; Strengthen abilities such as disease-resistant, but in improving painted and the hyperchromic feed of aquarium fish add hyperchromic element more, and these hyperchromic elements price height not only; Also can cause water pollution, environmental disruption simultaneously.Be unfavorable for viewing and admiring the healthy Sustainable development of fishery.
Summary of the invention
The object of the invention is microbial forage additive that provides a kind of enhancing color of pet fish and preparation method thereof.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is:
A kind of microbial forage additive of enhancing color of pet fish: form by Candida utilis, enterococcus faecalis, Lactobacterium acidophilum and subtilis; Then in substratum, be cultured to pH value 2.5-3.5; Be fodder additives, count Candida utilis by weight: enterococcus faecalis: Lactobacterium acidophilum: subtilis=1-3: 1-3: 1-3: 1-3.
Said substratum is agar 0.5～1.5g, yeast 0.5～1g, Carnis Bovis seu Bubali cream 0.5～1.5g, yeast extract 2～3g, sucrose 30～45g, skimmer 1.5～3.0g, peptone 0.5～1.5g, zero(ppm) water 1000mL.pH3.0～5.0。Said fodder additives and aquarium fish basal feed are fed after mixing with the proportioning of aquarium fish basal feed 1～3 ‰.
Preparing method: the Candida utilis behind the multiplication culture, enterococcus faecalis, Lactobacterium acidophilum and subtilis are counted Candida utilis by weight: enterococcus faecalis: Lactobacterium acidophilum: subtilis==1-3: 1-3: 1-3: 1-3; After above-mentioned bacterial classification mixed by mass percentage 10～15% in substratum ferment at constant temperature; And the logical simultaneously oxygen of stirring; The temperature initial temperature of cultivating is 28 ℃ and cultivated 24 hours; Cultivate 24 hours, 35 ℃ for 30 ℃ and cultivated 12 hours, get final product to pH value 2.5-3.5 until fermentation culture.Said substratum is agar 0.5～1.5g, yeast 0.5～1g, Carnis Bovis seu Bubali cream 0.5～1.5g, yeast extract 2～3g, sucrose 30～45g, skimmer 1.5～3.0g, peptone 0.5～1.5g, zero(ppm) water 1000mL.pH3.0～5.0。
The advantage that the present invention had:
The hyperchromic fodder additives of the present invention mixes with the aquarium fish basal feed throws something and feeds; Can significantly improve the painted and hyperchromic of aquarium fish; Strengthen color sensation, improve the ornamental value of aquarium fish, the cultivation water of aquarium fish and the intestinal microflora of aquarium fish, immune resistibility etc. are all had good regulating effect.
Hyperchromic fodder additives of the present invention not only has tangible enhancement to the color sensation of aquarium fish, simultaneously, can improve the dissolved oxygen in the water, significantly reduces the concentration of objectionable impuritiess such as ammonia-state nitrogen, nitrite nitrogen and hydrogen sulfide, safety and environmental protection, drug residue free.
Embodiment
Embodiment 1
Preparing method: the Candida utilis behind the multiplication culture, enterococcus faecalis, Lactobacterium acidophilum and subtilis are counted Candida utilis by weight: enterococcus faecalis: Lactobacterium acidophilum: subtilis==1: 1: 1: 1; After above-mentioned bacterial classification mixed by mass percentage 10～15% in substratum ferment at constant temperature; And the logical simultaneously oxygen of stirring; The temperature initial temperature of cultivating is 28 ℃ and cultivated 24 hours; Cultivate 24 hours, 35 ℃ for 30 ℃ and cultivated 12 hours, get final product until fermentation culture to pH value 2.5.
Said substratum is agar 1.5g, yeast 1g, Carnis Bovis seu Bubali cream 1g, yeast extract 2g, sucrose 35g, skimmer (commercial product, present embodiment is available from U.S. occasion limited-liability company) 2.0g, peptone 1g, zero(ppm) water 1000mL, pH3.0～5.0.Said yeast extract is that yeast soaks powder (commercial product, present embodiment yeast soak powder available from the extensive and profound in meaning star in Beijing Bioisystech Co., Ltd).
Said Candida utilis, enterococcus faecalis, Lactobacterium acidophilum and subtilis carry out multiplication culture according to ordinary method in following substratum separately.
Said Candida utilis multiplication culture soaks powder (commercial) 3g in yam, and glucose 20g, agar powder 15g, zero(ppm) water add in the 1000ml substratum, and 121 ℃ of autoclavings 20 minutes are subsequent use.
Said enterococcus faecalis multiplication culture is in glucose 2%, peptone 1%, Carnis Bovis seu Bubali cream 1%, yeast extract paste 0.5%; Potassium hydrogenphosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%; Sal epsom 0.058%, manganous sulfate 0.025%, agar 1.5-2%; In the pH6.5 substratum, 121 ℃ of autoclavings 20 minutes, subsequent use.
Said Lactobacterium acidophilum multiplication culture is in glucose 2%, peptone 1%, Carnis Bovis seu Bubali cream 1%, yeast extract paste 0.5%; Potassium hydrogenphosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%; Sal epsom 0.058%, manganous sulfate 0.025%, agar 1.5-2%; In the pH6.5 substratum, 121 ℃ of autoclavings 20 minutes, subsequent use.Said subtilis multiplication culture is in peptone 10g, agar 20g, and sodium-chlor 5g, meat soak powder 10g, and water adds to 1000ml, in the pH7.2 substratum, 121 ℃ of autoclavings 20 minutes, subsequent use.
Application examples
Selecting market conventional cultivation kind rose cockatto fish is test materials; (available from the beautiful red Shui nationality in Taiwan Province ltd) mixed with the hyperchromic fodder additives of above-mentioned gained and aquarium fish foundation bait; Add rose cockatto aquarium fish of throwing something and feeding after the hyperchromic fodder additives of above-mentioned gained mixes with aquarium fish foundation bait 3 ‰ ratios, through after the feeding an of week, test group is compared with control group; Test group rose cockatto fish color sensation strengthens; Significantly improve the painted and hyperchromic of rose cockatto fish, color and luster is vivider, and ornamental value has raising significantly.
Embodiment 2
Preparing method: the Candida utilis behind the multiplication culture, enterococcus faecalis, Lactobacterium acidophilum and subtilis are counted Candida utilis by weight: enterococcus faecalis: Lactobacterium acidophilum: subtilis==1: 2: 2: 1; After above-mentioned bacterial classification mixed by mass percentage 15% in substratum ferment at constant temperature; And the logical simultaneously oxygen of stirring; The temperature initial temperature of cultivating is 28 ℃ and cultivated 24 hours; Cultivate 24 hours, 35 ℃ for 30 ℃ and cultivated 12 hours, get final product until fermentation culture to pH value 3.Said substratum is agar 0.5g, Carnis Bovis seu Bubali cream 0.5g, yeast extract 2g, sucrose 30g, skimmer 3.0g, peptone 0.5g, zero(ppm) water 1000mL.PH3.0～5.0,110～130 ℃ autoclaving.Said yeast extract is that yeast soaks powder.
Application examples
Selecting the red swordfish of market conventional cultivation kind aquarium fish is test materials, uses " Eating God is red " bait (available from Haifeng Fodder Co., Ltd.) to be foundation bait, adds the red swordfish of throwing something and feeding after the hyperchromic fodder additives of above-mentioned gained mixes with aquarium fish foundation bait 1 ‰ ratios; Through one the week feed after; Test group is compared with control group, and the red swordfish color and luster of test group is scarlet, and body colour is even; Color sensation obviously strengthens, and has sight more.
Embodiment 3
Preparing method: the Candida utilis behind the multiplication culture, enterococcus faecalis, Lactobacterium acidophilum and subtilis are counted Candida utilis by weight: enterococcus faecalis: Lactobacterium acidophilum: subtilis==3: 1: 1: 1; After above-mentioned bacterial classification mixed by mass percentage 12% in substratum ferment at constant temperature; And the logical simultaneously oxygen of stirring; The temperature initial temperature of cultivating is 28 ℃ and cultivated 24 hours; Cultivate 24 hours, 35 ℃ for 30 ℃ and cultivated 12 hours, get final product until fermentation culture to pH value 3.5.
Said substratum is agar 1.5g, Carnis Bovis seu Bubali cream 1.5g, yeast extract 2g, sucrose 45g, skimmer 2g, peptone 1.5g, zero(ppm) water 1000mL.PH3.0～5.0,110～130 ℃ autoclaving.
Embodiment 4
Preparing method: the Candida utilis behind the multiplication culture, enterococcus faecalis, Lactobacterium acidophilum and subtilis are counted Candida utilis by weight: enterococcus faecalis: Lactobacterium acidophilum: subtilis==1: 2: 2: 1; After above-mentioned bacterial classification mixed by mass percentage 14% in substratum ferment at constant temperature; And the logical simultaneously oxygen of stirring; The temperature initial temperature of cultivating is 28 ℃ and cultivated 24 hours; Cultivate 24 hours, 35 ℃ for 30 ℃ and cultivated 12 hours, get final product until fermentation culture to pH value 3.3.Said substratum is agar 1.2g, Carnis Bovis seu Bubali cream 1.3g, yeast extract 2.8g, sucrose 40g, skimmer 2g, peptone 0.8g, zero(ppm) water 1000mL.PH3.0～5.0,110～130 ℃ autoclaving.
Claims (4)
1. the microbial forage additive of an enhancing color of pet fish; It is characterized in that: form by Candida utilis, enterococcus faecalis, Lactobacterium acidophilum and subtilis; Then in substratum, be cultured to the strong acid attitude environment of pH value 2.5-3.5, count Candida utilis by weight: enterococcus faecalis: Lactobacterium acidophilum: subtilis=1-3: 1-3: 1-3: 1-3;
Said fodder additives and aquarium fish basal feed are fed after mixing with the proportioning of aquarium fish basal feed 1～3 ‰;
After above-mentioned bacterial classification mixed by mass percentage 10～15% in substratum ferment at constant temperature; And the logical simultaneously oxygen of stirring; The temperature initial temperature of cultivating is 28 ℃ cultivated 24 hours, cultivated 24 hours, 35 ℃ for 30 ℃ and cultivated 12 hours, got final product to pH value 2.5-3.5 until fermentation culture.
2. by the microbial forage additive of the described enhancing color of pet fish of claim 1, it is characterized in that: said substratum is agar 0.5～1.5g, yeast 0.5～1g; Carnis Bovis seu Bubali cream 0.5～1.5g, yeast extract 2～3g, sucrose 30～45g; Skimmer 1.5～3.0g; Peptone 0.5～1.5g, zero(ppm) water 1000mL, pH3.0～5.0.
3. preparation method by the microbial forage additive of the described enhancing color of pet fish of claim 1; It is characterized in that: the Candida utilis behind the multiplication culture, enterococcus faecalis, Lactobacterium acidophilum and subtilis are counted Candida utilis by weight: enterococcus faecalis: Lactobacterium acidophilum: subtilis=1-3: 1-3: 1-3: 1-3; After above-mentioned bacterial classification mixed by mass percentage 10～15% in substratum ferment at constant temperature; And the logical simultaneously oxygen of stirring; The temperature initial temperature of cultivating is 28 ℃ cultivated 24 hours, cultivated 24 hours, 35 ℃ for 30 ℃ and cultivated 12 hours, got final product to pH value 2.5-3.5 until fermentation culture.
4. press the preparation method of the microbial forage additive of the described enhancing color of pet fish of claim 3, it is characterized in that: said substratum is agar 0.5～1.5g, yeast 0.5～1g; Carnis Bovis seu Bubali cream 0.5～1.5g, yeast extract 2～3g, sucrose 30～45g; Skimmer 1.5～3.0g; Peptone 0.5～1.5g, zero(ppm) water 1000mL, pH3.0～5.0.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009102489172A CN101803679B (en) | 2009-12-30 | 2009-12-30 | Micro-ecological feed additive for enhancing color of pet fish and preparation method thereof |
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| CN2009102489172A CN101803679B (en) | 2009-12-30 | 2009-12-30 | Micro-ecological feed additive for enhancing color of pet fish and preparation method thereof |
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| CN101803679B true CN101803679B (en) | 2012-06-27 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102640863B (en) * | 2012-02-09 | 2013-04-17 | 西华大学 | Preparation method of micro-ecological preparation for ornamental fish feed |
| CN103621792A (en) * | 2013-11-29 | 2014-03-12 | 吉林省科技评估有限责任公司 | Feed additive containing L-lysine composite probiotics and preparation method thereof |
| CN103749353A (en) * | 2014-01-03 | 2014-04-30 | 中国水产科学研究院珠江水产研究所 | Method of reddening bodies of aquarium fishes through albumen gland of Pomacea canaliculata females |
| EP3612021A4 (en) * | 2017-04-20 | 2021-04-14 | Locus IP Company, LLC | Cost-effective compositions and methods for enhanced aquatic farming and aquarium fish husbandry |
| CN109156667A (en) * | 2018-09-28 | 2019-01-08 | 河南科技大学 | A kind of fish hyperchromic feed and the preparation method and application thereof |
| CN114128808A (en) * | 2020-09-03 | 2022-03-04 | 河北科星药业有限公司 | Fish liquid feed additive capable of promoting digestion and preparation method and application thereof |
| CN112314778A (en) * | 2020-11-25 | 2021-02-05 | 天津科润达生物工程研究有限公司 | Compound probiotic fermented Chinese herbal medicine feed for aquarium fish and preparation method thereof |
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