CN102640863B - Preparation method of micro-ecological preparation for ornamental fish feed - Google Patents
Preparation method of micro-ecological preparation for ornamental fish feed Download PDFInfo
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- CN102640863B CN102640863B CN 201210028243 CN201210028243A CN102640863B CN 102640863 B CN102640863 B CN 102640863B CN 201210028243 CN201210028243 CN 201210028243 CN 201210028243 A CN201210028243 A CN 201210028243A CN 102640863 B CN102640863 B CN 102640863B
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Abstract
The invention discloses a micro-ecological preparation for an ornamental fish feed and a preparation method of the micro-ecological preparation, and belongs to a micro-ecological preparation, which comprises an embedding medium prepared by mixing a lactobacillus acidophilus suspension which is subjected to extended culture by a culture medium and has a culture inoculation rate of 8-12%, as well as beta-cyclodextrin, chitosan, whey protein concentrate and porous starch which respectively accounts for 10-15%, 12-18%, 3-5% and 8-10% of the total amount of the lactobacillus acidophilus suspension, or as well as chitosan, whey protein concentrate and carbohydrate which respectively accounts for 12-20%, 5-8% and 6-10 % of the total amount of the lactobacillus acidophilus suspension. The preparation method of the micro-ecological preparation for the ornamental fish feed has the advantages of simple process step, low cost of used materials and broad application range, and is suitable for industrial production.
Description
Technical field
The present invention relates to a kind of microorganism formulation, say more specifically a kind of preparation method of probiotics of raising for pet fish.
Background technology
Probiotics mainly is the living microbe formulation that utilizes the material of normal microorganism or accelerate growth of microorganism to make.The research of domestic animal microecological formulation starts from 20th century the seventies, what bright Puritanism by Sichuan Agricultural University is awarded the proposition that takes the lead in, and at first succeeded in developing the livestock and poultry fish microbe additives such as 8701,8801,8901 in 1987, started at home the microbe additive beginning.And at present, the probiotics micro-ecological formulation industry at home or an emerging industry, the starting stage of being at an early stage of development, development abroad relatively better, but the development time of whole industry is all also not long.Animal probio commonly used has in city's pin product at present: bacillus, lactic acid bacteria, saccharomycete, streptococcus fecalis etc.And the probio pulvis of domestic use such as lactobacillus preparation all are from external import basically, and home products is extremely rare, and the pet fish feeding micro-ecological preparation just still less.Probiotic composition in the market, majority is the trophosome cell of non-embedding, therefore, ubiquity keeping quality poor, prolong the shortcoming that number of viable reduction etc. is difficult to overcome with the holding time, the application of product is restricted, brings many negative effects to industry development.Therefore, be necessary to make further research and improvement for aforesaid defective for the pet fish feeding micro-ecological preparation.
Summary of the invention
The object of the invention is to solve above-mentioned deficiency, a kind of embedding medium that has is provided, and the preparation method of a kind of probiotics of raising for pet fish that is conducive to fancy fishes growths.
For solving above-mentioned technical problem, the present invention by the following technical solutions:
One aspect of the present invention provides a kind of probiotics of raising for pet fish, comprise in the described preparation through culture medium and enlarge cultivation, and cultivate rate of vaccination and be 8% to 12% lactobacillus acidophilus suspension and 10% to 15% the beta-schardinger dextrin-that accounts for lactobacillus acidophilus suspension total amount, 12% to 18% shitosan, 3% to 5% WPC, the embedding medium that 8% to 10% porous-starch mixes; Or with 12% to 20% the shitosan that accounts for lactobacillus acidophilus suspension total amount, 5% to 8% WPC, the embedding medium that 6% to 10% carbohydrate mixes.
The present invention provides a kind of preparation method of probiotics of raising for pet fish on the other hand, and described method operates in accordance with the following steps take lactobacillus acidophilus as main preparation probiotics:
The expansion of step 1, lactobacillus acidophilus is cultivated, and the good strain of seed selection lactobacillus acidophilus places culture medium to cultivate 30 to 40 degrees centigrade temperature and namely made the lactobacillus acidophilus suspension in 20 to 28 hours; Comprise 8 to 12 parts of peptones in the described culture medium, 5 to 7 parts of yeast extracts, 10 to 14 parts of beef extracts, 14 to 18 parts of glucose, 4 to 6 parts of sodium acetates, 2 to 2.5 parts of ammonium citrates, Tween800.8 to 1.2 part, MgSO
47H
2O0.55 to 0.6 part, MnSO
44H
20.04 to 0.06 part of O, K
2HPO41.8 to 2.2 part, 16 to 18 parts of agar powders, 900 to 1000 parts in water, the cultivation rate of vaccination of described lactobacillus acidophilus is 8% to 12%;
The wall material of step 2, lactobacillus acidophilus probiotics is selected, after enlarging the cultivation end, the lactobacillus acidophilus suspension is filtered, and add in the lactobacillus acidophilus suspension after filtration account for filter after 10% to 15% beta-schardinger dextrin-of lactobacillus acidophilus suspension total amount, 12% to 18% shitosan, 3% to 5% WPC, 8% to 10% porous-starch also mixes; Perhaps add account for filter after 12% to 20% shitosan of lactobacillus acidophilus suspension total amount, 5% to 8% WPC, 6% to 10% carbohydrate also mixes, and namely obtains adding the lactobacillus acidophilus suspension behind the embedding medium;
Step 3, the lactobacillus acidophilus suspension that adds behind the embedding medium is carried out namely obtaining lactobacillus acidophilus probiotics finished product after the drying.
Further technical scheme is: the pH acid-base value initial value of the culture medium that adopts in the described step 1 is 5.5.
Further technical scheme is: the drying means in the described step 3 is the low temperature spray drying method, lactobacillus acidophilus suspension behind the interpolation embedding medium is put into sprayer unit, the inlet temperature of device is 85 to 95 degrees centigrade, outlet temperature is 55 to 65 degrees centigrade, and be 3 to 6 seconds the average time that the lactobacillus acidophilus suspension behind the interpolation embedding medium is accepted to spray in sprayer unit.
Further technical scheme is: the drying means in the described step 3 is freeze-drying, the lactobacillus acidophilus suspension that adds behind the embedding medium is placed subzero low temperature refrigerator pre-freeze below 40 degrees centigrade 3 to 7 hours, and then be placed in the freeze drying plant that vacuum is 15 to 25 handkerchiefs freeze drying 30 to 40 hours.
Compared with prior art, the invention has the beneficial effects as follows: cultivate the good strain of lactobacillus acidophilus by adopting to enlarge, select shitosan, the lactalbumin embedding that combines with carbohydrate, perhaps adopt beta-schardinger dextrin-, shitosan, WPC, the probiotics that is exclusively used in fancy fishes has been developed in the porous-starch embedding that combines.And experimental result shows, microorganism formulation of the present invention is used for pet fish raises, the pet fish speed of growth is obviously accelerated, stocking density can increase by 30%, survival rate improves 50% to 60%, and the color of fancy fishes becomes more bright-coloured, so that pet fish has good economic worth, has more ornamental value.And a kind of probiotics preparation method who raises for pet fish provided by the present invention, processing step is simple, and the material therefor cost is low, is suitable for suitability for industrialized production, and range of application is wide.
The specific embodiment
The present invention is further elaborated below in conjunction with accompanying drawing.
The first embodiment of the present invention is that the one-tenth that a kind of probiotics of raising for pet fish will be described is grouped into, comprise in the described preparation through culture medium and enlarge cultivation, and the cultivation rate of vaccination is 8% to 12% lactobacillus acidophilus suspension, with 10% to 15% the beta-schardinger dextrin-that accounts for lactobacillus acidophilus suspension total amount, 12% to 18% shitosan, 3% to 5% WPC, the embedding medium that 8% to 10% porous-starch mixes; Or with 12% to 20% the shitosan that accounts for lactobacillus acidophilus suspension total amount, 5% to 8% WPC, the embedding medium that 6% to 10% carbohydrate mixes.
The second embodiment of the present invention is the preparation method that above-mentioned probiotics of raising for pet fish will be described, described method operates in accordance with the following steps take lactobacillus acidophilus as main preparation probiotics:
The expansion of step 1, lactobacillus acidophilus is cultivated, and the good strain of seed selection lactobacillus acidophilus places culture medium to cultivate 30 to 40 degrees centigrade temperature and namely made the lactobacillus acidophilus suspension in 20 to 28 hours; Comprise 8 to 12 parts of peptones in the described culture medium, 5 to 7 parts of yeast extracts, 10 to 14 parts of beef extracts, 14 to 18 parts of glucose, 4 to 6 parts of sodium acetates, 2 to 2.5 parts of ammonium citrates, Tween800.8 to 1.2 part, MgSO
47H
2O0.55 to 0.6 part, MnSO
44H
20.04 to 0.06 part of O, K
2HPO41.8 to 2.2 part, 16 to 18 parts of agar powders, 900 to 1000 parts in water, the cultivation rate of vaccination of described lactobacillus acidophilus is 8% to 12%;
When disposing above-mentioned culture medium, pay particular attention to its initial pH value, scope such as pH value arranges incorrect, just can affect the effect of the expansion cultivation of lactobacillus acidophilus, and the present inventor is through experiment, think that the culture medium initial p H value of comparative optimization is 5.5, but need to prove that this only is that the inventor tests better parameter, the pH value that fluctuates in this scope can both reach same or analogous effect, helps lactobacillus acidophilus to enlarge and cultivates.
The wall material of step 2, lactobacillus acidophilus probiotics is selected, after enlarging the cultivation end, the lactobacillus acidophilus suspension is filtered, and add in the lactobacillus acidophilus suspension after filtration account for filter after 10% to 15% beta-schardinger dextrin-of lactobacillus acidophilus suspension total amount, 12% to 18% shitosan, 3% to 5% WPC, 8% to 10% porous-starch also mixes; Perhaps add account for filter after 12% to 20% shitosan of lactobacillus acidophilus suspension total amount, 5% to 8% WPC, 6% to 10% carbohydrate also mixes, and namely obtains adding the lactobacillus acidophilus suspension behind the embedding medium;
Step 3, the lactobacillus acidophilus suspension that adds behind the embedding medium is carried out namely obtaining lactobacillus acidophilus probiotics finished product after the drying.
For in the above-mentioned steps three the lactobacillus acidophilus suspension behind the interpolation embedding medium being carried out dry mode, the present inventor thinks that with reference to various drying processes of the prior art following two kinds of drying modes all can reach the drying effect of expection.
These two kinds of drying modes are a kind of for adopting the low temperature spray drying method, lactobacillus acidophilus suspension behind the interpolation embedding medium is put into sprayer unit, the inlet temperature of device is 85 to 95 degrees centigrade, outlet temperature is 55 to 65 degrees centigrade, and be 3 to 6 seconds the average time that the lactobacillus acidophilus suspension behind the interpolation embedding medium is accepted to spray in sprayer unit.Because the bad control of accurate temperature of sprayer unit, what the inventor adopted when experiment is the sprayer unit of 3.5 meters of tower heights, and any one parameter value in the aforesaid temperature range value all can be realized the drying effect of this kind method, and the lactobacillus acidophilus suspension behind the interpolation embedding medium is accepted to be preferably 4.5 seconds in sprayer unit the average time of spraying.
Another kind of for adopting freeze-drying, the lactobacillus acidophilus suspension that adds behind the embedding medium is placed subzero 40 degrees centigrade low temperature refrigerator pre-freeze 3 to 7 hours, and then be placed in the freeze drying plant that vacuum is 15 to 25 handkerchiefs freeze drying 30 to 40 hours.The present inventor has carried out repeatedly realizing to this kind seasoning equally, think that the parameter value of comparative optimization in the above-mentioned parameter scope is pre-freeze under subzero 40 degrees centigrade low temperature 5 hours, and then be placed on that freeze drying namely realized dry in 36 hours in the freeze drying plant of vacuum 20 handkerchiefs.Need to prove still that herein the aforementioned parameters value can be used as a preferred embodiment of the present invention, the inventor thinks that all parameter values in the above-mentioned value range all can realize the drying effect of this kind seasoning.
It more than is preferred embodiment of the present invention; it is not the restriction to protection domain of the present invention; any to a kind of probiotics composition of raising for pet fish of the present invention interpolation or etc. matter replace; and the matter such as step that its preparation method is done are replaced; or have no the delete step behavior of technique effect, all within protection scope of the present invention.
Claims (4)
1. preparation method who is used for the probiotics that pet fish raises, it is characterized in that: described method operates in accordance with the following steps take lactobacillus acidophilus as main preparation probiotics:
The expansion of step 1, lactobacillus acidophilus is cultivated, and the good strain of seed selection lactobacillus acidophilus places culture medium to cultivate 30 to 40 degrees centigrade temperature and namely made the lactobacillus acidophilus suspension in 20 to 28 hours; Comprise 8 to 12 parts of peptones in the described culture medium, 5 to 7 parts of yeast extracts, 10 to 14 parts of beef extracts, 14 to 18 parts of glucose, 4 to 6 parts of sodium acetates, 2 to 2.5 parts of ammonium citrates, 0.8 to 1.2 part of Tween80, MgSO
47H
20.55 to 0.6 part of O, MnSO
44H
20.04 to 0.06 part of O, K
2HPO
41.8 to 2.2 parts, 16 to 18 parts of agar powders, 900 to 1000 parts in water, the cultivation rate of vaccination of described lactobacillus acidophilus is 8% to 12%;
The wall material of step 2, lactobacillus acidophilus probiotics is selected, after enlarging the cultivation end, the lactobacillus acidophilus suspension is filtered, and add in the lactobacillus acidophilus suspension after filtration account for filter after 10% to 15% beta-schardinger dextrin-of lactobacillus acidophilus suspension total amount, 12% to 18% shitosan, 3% to 5% WPC, 8% to 10% porous-starch also mixes; Perhaps add account for filter after 12% to 20% shitosan of lactobacillus acidophilus suspension total amount, 5% to 8% WPC, 6% to 10% carbohydrate also mixes, and namely obtains adding the lactobacillus acidophilus suspension behind the embedding medium;
Step 3, the lactobacillus acidophilus suspension that adds behind the embedding medium is carried out namely obtaining lactobacillus acidophilus probiotics finished product after the drying.
2. the preparation method of probiotics of raising for pet fish according to claim 1, it is characterized in that: the pH acid-base value initial value of the culture medium that adopts in the described step 1 is 5.5.
3. the preparation method of probiotics of raising for pet fish according to claim 1, it is characterized in that: the drying means in the described step 3 is the low temperature spray drying method, lactobacillus acidophilus suspension behind the interpolation embedding medium is put into sprayer unit, the inlet temperature of device is 85 to 95 degrees centigrade, outlet temperature is 55 to 65 degrees centigrade, and be 3 to 6 seconds the average time that the lactobacillus acidophilus suspension behind the interpolation embedding medium is accepted to spray in sprayer unit.
4. the preparation method of probiotics of raising for pet fish according to claim 1, it is characterized in that: the drying means in the described step 3 is freeze-drying, the lactobacillus acidophilus suspension that adds behind the embedding medium is placed subzero low temperature refrigerator pre-freeze below 40 degrees centigrade 3 to 7 hours, and then be placed in the freeze drying plant that vacuum is 15 to 25 handkerchiefs freeze drying 30 to 40 hours.
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102919542B (en) * | 2012-11-13 | 2014-02-05 | 西华大学 | Multistage-controlled-release probiotics microecological preparation composite feed for Silurus meridionalis and preparation method thereof |
| CN103478505A (en) * | 2013-10-15 | 2014-01-01 | 南京市水产科学研究所 | Soft granulated feed and preparation method thereof |
| CN104489268A (en) * | 2014-12-09 | 2015-04-08 | 中国农业科学院兰州畜牧与兽药研究所 | Micro-ecological preparation and preparation method thereof |
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| CN101904420A (en) * | 2010-09-02 | 2010-12-08 | 湖南农业大学 | Lactic acid bacterium or yeast porous starch embedding and protecting method |
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