CN102640863A - Micro-ecological preparation for ornamental fish feed and preparation method of micro-ecological preparation - Google Patents
Micro-ecological preparation for ornamental fish feed and preparation method of micro-ecological preparation Download PDFInfo
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- CN102640863A CN102640863A CN2012100282437A CN201210028243A CN102640863A CN 102640863 A CN102640863 A CN 102640863A CN 2012100282437 A CN2012100282437 A CN 2012100282437A CN 201210028243 A CN201210028243 A CN 201210028243A CN 102640863 A CN102640863 A CN 102640863A
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Abstract
The invention discloses a micro-ecological preparation for an ornamental fish feed and a preparation method of the micro-ecological preparation, and belongs to a micro-ecological preparation, which comprises an embedding medium prepared by mixing a lactobacillus acidophilus suspension which is subjected to extended culture by a culture medium and has a culture inoculation rate of 8-12%, as well as beta-cyclodextrin, chitosan, whey protein concentrate and porous starch which respectively accounts for 10-15%, 12-18%, 3-5% and 8-10% of the total amount of the lactobacillus acidophilus suspension, or as well as chitosan, whey protein concentrate and carbohydrate which respectively accounts for 12-20%, 5-8% and 6-10 % of the total amount of the lactobacillus acidophilus suspension. The preparation method of the micro-ecological preparation for the ornamental fish feed has the advantages of simple process step, low cost of used materials and broad application range, and is suitable for industrial production.
Description
Technical field
The present invention relates to a kind of microorganism formulation, a kind of probiotics that pet fish raises and preparation method thereof that is used for of saying so more specifically.
Background technology
Probiotics mainly is to utilize normal microorganism or promote the living microbe formulation that the material of growth of microorganism is processed.The research of domestic animal microecological formulation starts from 20th century the seventies; What bright Puritanism by Sichuan Agricultural University is awarded the proposition that takes the lead in; And at first succeeded in developing livestock and poultry fishes such as 8701,8801,8901 in 1987 and use microbe additive, started the microbe additive beginning at home.And at present, the probiotics micro-ecological formulation industry is still emerging industry at home, the starting stage of being at an early stage of development, and development abroad better relatively, but the development time of whole industry is all also not long.Animal probio commonly used has in city's pin product at present: bacillus, lactic acid bacteria, saccharomycete, streptococcus fecalis etc.And the probio pulvis of domestic use such as lactobacillus preparation all are from external import basically, and home products is extremely rare, and the pet fish feeding micro-ecological preparation just still less.Probiotic composition in the market; Majority is the trophosome cell of non-embedding, therefore, ubiquity keeping quality poor, prolong the shortcoming that number of viable reduction etc. is difficult to overcome with the holding time; The application of product is restricted, brings many negative effects to industry development.Therefore, be necessary to make further research and improvement to the pet fish feeding micro-ecological preparation to aforesaid defective.
Summary of the invention
The objective of the invention is to solve above-mentioned deficiency, a kind of embedding medium that has is provided, and a kind of probiotics that pet fish raises and preparation method thereof that is used for that helps fancy fishes growths.
For solving above-mentioned technical problem, the present invention adopts following technical scheme:
One aspect of the present invention provides a kind of probiotics that pet fish is raised that is used for; Comprise in the described preparation through the culture medium enlarged culture; And cultivate rate of vaccination and be 8% to 12% lactobacillus acidophilus suspension and 10% to 15% the beta-schardinger dextrin-that accounts for lactobacillus acidophilus suspension total amount; 12% to 18% shitosan, 3% to 5% WPC, the embedding medium that 8 to 10% porous-starch mixes; Or with 12% to 20% the shitosan that accounts for lactobacillus acidophilus suspension total amount, 5% to 8% WPC, the embedding medium that 6% to 10% carbohydrate mixes.
The present invention provides a kind of preparation method who is used for the probiotics that pet fish raises on the other hand, and described method is main preparation probiotics with the lactobacillus acidophilus, operates according to following steps:
The enlarged culture of step 1, lactobacillus acidophilus, the good strain of seed selection lactobacillus acidophilus place culture medium to cultivate 30 to 40 degrees centigrade temperature and promptly made the lactobacillus acidophilus suspension in 20 to 28 hours; Comprise 8 to 12 parts of peptones in the described culture medium, 5 to 7 parts of yeast extracts, 10 to 14 parts of beef extracts, 14 to 18 parts of glucose, 4 to 6 parts of sodium acetates, 2 to 2.5 parts of ammonium citrates, Tween800.8 to 1.2 part, MgSO
47H
2O0.55 to 0.6 part, MnSO
44H
20.04 to 0.06 part of O, K
21.8 to 2.2 parts of HPO4,16 to 18 parts of agar powders, 900 to 1000 parts in water, the cultivation rate of vaccination of described lactobacillus acidophilus is 8% to 12%;
The wall material of step 2, lactobacillus acidophilus probiotics is selected; After enlarged culture finishes; The lactobacillus acidophilus suspension is filtered, and add in the lactobacillus acidophilus suspension after filtration and account for 10% to 15% the beta-schardinger dextrin-that filters back lactobacillus acidophilus suspension total amount, 12% to 18% shitosan; 3% to 5% WPC, 8 to 10% porous-starch also mixes; Perhaps add accounting for 12% to 20% the shitosan that filters back lactobacillus acidophilus suspension total amount, 5% to 8% WPC, 6% to 10% carbohydrate also mixes, and promptly obtains adding the lactobacillus acidophilus suspension behind the embedding medium;
Step 3, the lactobacillus acidophilus suspension that adds behind the embedding medium is carried out promptly arriving lactobacillus acidophilus probiotics finished product after the drying.
Further technical scheme is: the pH acid-base value initial value of the culture medium that is adopted in the described step 1 is 5.5.
Technical scheme further is: the drying means in the described step 3 is the low temperature spray drying method; Lactobacillus acidophilus suspension behind the interpolation embedding medium is put into sprayer unit; The inlet temperature of device is 85 to 95 degrees centigrade; Outlet temperature is 55 to 65 degrees centigrade, and be 3 to 6 seconds the average time that the lactobacillus acidophilus suspension behind the interpolation embedding medium is accepted to spray in sprayer unit.
Technical scheme further is: the drying means in the described step 3 is a freeze-drying; The lactobacillus acidophilus suspension that adds behind the embedding medium is placed subzero low temperature refrigerator pre-freeze below 40 degrees centigrade 3 to 7 hours, and then be placed in the freeze drying plant that vacuum is 15 to 25 handkerchiefs freeze drying 30 to 40 hours.
Compared with prior art, the invention has the beneficial effects as follows:, select shitosan for use through adopting the good strain of enlarged culture lactobacillus acidophilus; The lactalbumin embedding that combines with carbohydrate; Perhaps adopt beta-schardinger dextrin-, shitosan, WPC; The probiotics that is exclusively used in fancy fishes has been developed in the porous-starch embedding that combines.And experimental result shows; Microorganism formulation according to the invention is used for pet fish raises, the pet fish speed of growth is obviously accelerated, stocking density can increase by 30%; Survival rate improves 50% to 60%; And the color of fancy fishes becomes more bright-coloured, makes pet fish have good economic worth, has more ornamental value.And a kind of probiotics preparation method that pet fish is raised that is used for provided by the present invention, processing step is simple, and the material therefor cost is low, is suitable for suitability for industrialized production, and range of application is wide.
The specific embodiment
Do further elaboration in the face of the present invention down.
First kind of embodiment of the present invention is to explain that a kind of one-tenth that is used for the probiotics that pet fish raises is grouped into; Comprise in the described preparation through the culture medium enlarged culture; And cultivate rate of vaccination and be 8% to 12% lactobacillus acidophilus suspension, with 10% to 15% the beta-schardinger dextrin-that accounts for lactobacillus acidophilus suspension total amount, 12% to 18% shitosan; 3% to 5% WPC, the embedding medium that 8 to 10% porous-starch mixes; Or with 12% to 20% the shitosan that accounts for lactobacillus acidophilus suspension total amount, 5% to 8% WPC, the embedding medium that 6% to 10% carbohydrate mixes.
Second kind of embodiment of the present invention is that the above-mentioned preparation method who is used for the probiotics that pet fish raises will be described, described method is main preparation probiotics with the lactobacillus acidophilus, operates according to following steps:
The enlarged culture of step 1, lactobacillus acidophilus, the good strain of seed selection lactobacillus acidophilus place culture medium to cultivate 30 to 40 degrees centigrade temperature and promptly made the lactobacillus acidophilus suspension in 20 to 28 hours; Comprise 8 to 12 parts of peptones in the described culture medium, 5 to 7 parts of yeast extracts, 10 to 14 parts of beef extracts, 14 to 18 parts of glucose, 4 to 6 parts of sodium acetates, 2 to 2.5 parts of ammonium citrates, 0.8 to 1.2 part of Tween80, MgSO
47H
2O0.55 to 0.6 part, MnSO
44H
20.04 to 0.06 part of O, K
21.8 to 2.2 parts of HPO4,16 to 18 parts of agar powders, 900 to 1000 parts in water, the cultivation rate of vaccination of described lactobacillus acidophilus is 8% to 12%;
When the above-mentioned culture medium of configuration, pay particular attention to its initial pH value, be provided with like the scope of pH value incorrect, just can influence the effect of the enlarged culture of lactobacillus acidophilus; And inventor of the present invention is through experiment; Think that the culture medium initial p H value of comparative optimization is 5.5, but need to prove that this only is that the inventor tests preferable parameter; The pH value that fluctuates in this scope can both reach same or analogous effect, helps the lactobacillus acidophilus enlarged culture.
The wall material of step 2, lactobacillus acidophilus probiotics is selected; After enlarged culture finishes; The lactobacillus acidophilus suspension is filtered, and add in the lactobacillus acidophilus suspension after filtration and account for 10% to 15% the beta-schardinger dextrin-that filters back lactobacillus acidophilus suspension total amount, 12% to 18% shitosan; 3% to 5% WPC, 8 to 10% porous-starch also mixes; Perhaps add accounting for 12% to 20% the shitosan that filters back lactobacillus acidophilus suspension total amount, 5% to 8% WPC, 6% to 10% carbohydrate also mixes, and promptly obtains adding the lactobacillus acidophilus suspension behind the embedding medium;
Step 3, the lactobacillus acidophilus suspension that adds behind the embedding medium is carried out promptly arriving lactobacillus acidophilus probiotics finished product after the drying.
To in the above-mentioned steps three the lactobacillus acidophilus suspension behind the interpolation embedding medium being carried out dry mode, inventor of the present invention thinks that with reference to various drying processes of the prior art following two kinds of drying modes all can reach the drying effect of expection.
These two kinds of drying modes are a kind of for adopting the low temperature spray drying method; Lactobacillus acidophilus suspension behind the interpolation embedding medium is put into sprayer unit; The inlet temperature of device is 85 to 95 degrees centigrade; Outlet temperature is 55 to 65 degrees centigrade, and be 3 to 6 seconds the average time that the lactobacillus acidophilus suspension behind the interpolation embedding medium is accepted to spray in sprayer unit.Because the bad control of accurate temperature of sprayer unit; What the inventor adopted when experiment is the sprayer unit of 3.5 meters of tower heights; And any one parameter value in the aforesaid temperature range value all can be realized the drying effect of this kind method, and the lactobacillus acidophilus suspension behind the interpolation embedding medium is accepted to be preferably 4.5 seconds the average time of spraying in sprayer unit.
Another kind of for adopting freeze-drying, the lactobacillus acidophilus suspension that adds behind the embedding medium is placed subzero 40 degrees centigrade low temperature refrigerator pre-freeze 3 to 7 hours, and then be placed in the freeze drying plant that vacuum is 15 to 25 handkerchiefs freeze drying 30 to 40 hours.Inventor of the present invention has carried out repeatedly realizing to this kind seasoning equally; Think that the parameter value of comparative optimization in the above-mentioned parameter scope is pre-freeze under subzero 40 degrees centigrade low temperature 5 hours, and then be placed on that freeze drying promptly realized dry in 36 hours in the freeze drying plant of vacuum 20 handkerchiefs.Need to prove still that here the aforementioned parameters value can be used as a preferred implementation of the present invention, the inventor thinks that all parameter values in the above-mentioned value range all can realize the drying effect of this kind seasoning.
It more than is preferred implementation of the present invention; It is not qualification to protection domain of the present invention; Any to a kind of interpolation that is used for the probiotics composition that pet fish raises of the present invention or etc. the matter replacement; And the matter such as step that its preparation method is done replacement, or have no the deletion step behavior of technique effect, all within protection scope of the present invention.
Claims (5)
1. one kind is used for the probiotics that pet fish is raised; It is characterized in that: comprise in the described preparation through the culture medium enlarged culture; And cultivate rate of vaccination and be 8% to 12% lactobacillus acidophilus suspension and 10% to 15% the beta-schardinger dextrin-that accounts for lactobacillus acidophilus suspension total amount; 12% to 18% shitosan, 3% to 5% WPC, the embedding medium that 8 to 10% porous-starch mixes; Or with 12% to 20% the shitosan that accounts for lactobacillus acidophilus suspension total amount, 5% to 8% WPC, the embedding medium that 6% to 10% carbohydrate mixes.
2. preparation method who is used for the probiotics that pet fish raises, it is characterized in that: described method is main preparation probiotics with the lactobacillus acidophilus, operates according to following steps:
The enlarged culture of step 1, lactobacillus acidophilus, the good strain of seed selection lactobacillus acidophilus place culture medium to cultivate 30 to 40 degrees centigrade temperature and promptly made the lactobacillus acidophilus suspension in 20 to 28 hours; Comprise 8 to 12 parts of peptones in the described culture medium, 5 to 7 parts of yeast extracts, 10 to 14 parts of beef extracts, 14 to 18 parts of glucose, 4 to 6 parts of sodium acetates, 2 to 2.5 parts of ammonium citrates, 0.8 to 1.2 part of Tween80, MgSO
47H
20.55 to 0.6 part of O, MnSO
40.04 to 0.06 part of 4H2O, K
2HPO
41.8 to 2.2 parts, 16 to 18 parts of agar powders, 900 to 1000 parts in water, the cultivation rate of vaccination of described lactobacillus acidophilus is 8% to 12%;
The wall material of step 2, lactobacillus acidophilus probiotics is selected; After enlarged culture finishes; The lactobacillus acidophilus suspension is filtered, and add in the lactobacillus acidophilus suspension after filtration and account for 10% to 15% the beta-schardinger dextrin-that filters back lactobacillus acidophilus suspension total amount, 12% to 18% shitosan; 3% to 5% WPC, 8 to 10% porous-starch also mixes; Perhaps add accounting for 12% to 20% the shitosan that filters back lactobacillus acidophilus suspension total amount, 5% to 8% WPC, 6% to 10% carbohydrate also mixes, and promptly obtains adding the lactobacillus acidophilus suspension behind the embedding medium;
Step 3, the lactobacillus acidophilus suspension that adds behind the embedding medium is carried out promptly arriving lactobacillus acidophilus probiotics finished product after the drying.
3. the preparation method who is used for the probiotics that pet fish raises according to claim 2, it is characterized in that: the pH acid-base value initial value of the culture medium that is adopted in the described step 1 is 5.5.
4. the preparation method who is used for the probiotics that pet fish raises according to claim 2; It is characterized in that: the drying means in the described step 3 is the low temperature spray drying method; Lactobacillus acidophilus suspension behind the interpolation embedding medium is put into sprayer unit; The inlet temperature of device is 85 to 95 degrees centigrade, and outlet temperature is 55 to 65 degrees centigrade, and be 3 to 6 seconds the average time that the lactobacillus acidophilus suspension behind the interpolation embedding medium is accepted to spray in sprayer unit.
5. the preparation method who is used for the probiotics that pet fish raises according to claim 2; It is characterized in that: the drying means in the described step 3 is a freeze-drying; The lactobacillus acidophilus suspension that adds behind the embedding medium is placed subzero low temperature refrigerator pre-freeze below 40 degrees centigrade 3 to 7 hours, and then be placed in the freeze drying plant that vacuum is 15 to 25 handkerchiefs freeze drying 30 to 40 hours.
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| CN 201210028243 CN102640863B (en) | 2012-02-09 | 2012-02-09 | Preparation method of micro-ecological preparation for ornamental fish feed |
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| CN102919542A (en) * | 2012-11-13 | 2013-02-13 | 西华大学 | Multistage-controlled-release probiotics microecological preparation composite feed for Silurus meridionalis and preparation method thereof |
| CN103478505A (en) * | 2013-10-15 | 2014-01-01 | 南京市水产科学研究所 | Soft granulated feed and preparation method thereof |
| CN104489268A (en) * | 2014-12-09 | 2015-04-08 | 中国农业科学院兰州畜牧与兽药研究所 | Micro-ecological preparation and preparation method thereof |
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Cited By (3)
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| CN102919542A (en) * | 2012-11-13 | 2013-02-13 | 西华大学 | Multistage-controlled-release probiotics microecological preparation composite feed for Silurus meridionalis and preparation method thereof |
| CN103478505A (en) * | 2013-10-15 | 2014-01-01 | 南京市水产科学研究所 | Soft granulated feed and preparation method thereof |
| CN104489268A (en) * | 2014-12-09 | 2015-04-08 | 中国农业科学院兰州畜牧与兽药研究所 | Micro-ecological preparation and preparation method thereof |
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| CN102640863B (en) | 2013-04-17 |
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