CN103922847A - Pleurotus eryngii culture medium containing rubber tree sawdust and preparation method of pleurotus eryngii culture medium - Google Patents

Pleurotus eryngii culture medium containing rubber tree sawdust and preparation method of pleurotus eryngii culture medium Download PDF

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CN103922847A
CN103922847A CN201410159771.5A CN201410159771A CN103922847A CN 103922847 A CN103922847 A CN 103922847A CN 201410159771 A CN201410159771 A CN 201410159771A CN 103922847 A CN103922847 A CN 103922847A
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rubber tree
pleurotus eryngii
wood chip
fruit
tree wood
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CN103922847B (en
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张柏松
杨向黎
郑福丽
田叶
马征
边文范
徐长英
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Institute of Agricultural Resources and Regional Planning of CAAS
Institute of Agricultural Resources and Environment of Shandong Academy of Agricultural Sciences
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Institute of Agricultural Resources and Environment of Shandong Academy of Agricultural Sciences
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Abstract

The invention belongs to the technical scheme of mushroom cultivation and particularly relates to a pleurotus eryngii culture medium containing rubber tree sawdust and a preparation method of the pleurotus eryngii culture medium. The pleurotus eryngii culture medium containing rubber tree sawdust is characterized by comprising the following components: corncob, wheat bran, rubber tree sawdust, land plaster, lime, cottonseed hull, sugar, light calcium carbonate, mushroom residue, root and base of flammulina velutipes, palm kernel shell, soybean meal, cold pressed peanut meal, urea, manganese and copper. When the culture medium disclosed by the invention is used for cultivating pleurotus eryngii, hyphae grow vigorously and are pure white and dense, cultivation bags cannot be contaminated, pleurotus eryngii sporocarp has pure white prosthecae and round and regular shape, no deformed pleurotus eryngii grows out, biological efficiency reaches 108%.

Description

Contain culture material of the Pleurotus eryngii of rubber tree wood chip and preparation method thereof
Technical field
The invention belongs to mushroom class cultivation technique field, be specifically related to a kind of culture material of the Pleurotus eryngii containing rubber tree wood chip, also relate to its making method of above-mentioned culture material.
background technology
Pleurotus eryngii, has another name called eryngo and picks up the ears, and is to develop in recent years cultivation successfully to integrate rare edible mushrooms new variety edible, medicinal, dietotherapy.Pleurotus eryngii bacterial context plumpness, quality is tender and crisp, particularly stem dense structure, solid, milky white, can all eat, and stem, than the more crisp cunning of cap, tasty and refreshing, is called as " flat mushroom king ", " dried scallop mushroom ", there is happy almond flavor and as the mouthfeel of abalone, be applicable to fresh-keeping, processing, firmly get liking of people.Mushroom body has almond flavor, meat plumpness, and mouthfeel is fresh and tender, and taste delicate fragrance is nutritious, can cook tens road delicious foods.Also have reducing blood-fat, decreasing cholesterol, promotion gastro-intestinal digestion, enhancing body immunological competence, prevent that the effects such as cardiovascular diseases, the utmost point are liked by people, market value is than 5 times of the high 3-of flat mushroom.
The reproductive condition of Pleurotus eryngii is as follows:
1. nutrition: Pleurotus eryngii is a kind of decompose xylogen, edible mushrooms that Mierocrystalline cellulose ability is stronger, need to have richer nutrition, and particularly nitrogenous source is abundant, and mycelial growth is luxuriant, and output is higher.Tame major ingredient is grown fine in the matrix such as cotton seed hull, wood chip, bagasse, straw.Substituting stuff cultivation adds subsidiary material, and as rice bran, wheat bran, Semen Maydis powder, cotton seed powder cake, calcium carbonate, sugar etc., can promote mycelial growth spreads, and increases mushroom flower bud generating capacity.
2. moisture: Pleurotus eryngii is more drought-enduring, but suitable being more conducive to of water content grow, improve output.Vegetative stage, the suitable water content of culture material is 60%-65%, but because should not spray water on mushroom body in the time of cultivation, the required moisture of mushroom body mainly originates from culture material, so can appropriately be increased to 65%-70% while allocating culture material water content.Relative air humidity, mycelial growth stage 60% left and right, and after existing former base sporophore differential period be advisable with 90-95%, the sporophore growth stage can appropriately turn down 85-90%.
3. temperature: temperature is the key that determines planting almond abalone mushroom success or failure and output height.The mycelia growing of pleurotus eryngii temperature is 22-27 DEG C, and 25 DEG C of optimum temperutures, for a long time lower than 18 DEG C of easy fruitings.It is constant temperature fecundity mushroom, sporophore genesis and development temperature 10-20 DEG C, 15 DEG C of optimums.
4. air: vegetative stage oxygen requirement is relatively less, certain density CO 2mycelial growth is had to promoter action.Former base forms and sporophore growth wants air fresh, CO 2too much high meeting produces toxic action, between the sporophore growth growth period, and CO 2concentration is controlled at 0.03-0.2% and is advisable.
5. illumination: the mycelial growth stage does not need light can be accelerated mycelial growth under dark environment.The growth and development stage of existing former base and sporophore needs certain scattered light, and suitable intensity of illumination is 500-1000Lx, the blackening of light intensity cap; A little less than light, cap bleaches, and stem is elongated.
6. potential of hydrogen: the pH value field of mycelial growth is 4-8, and optimal pH is 6.5-7.5.The optimum pH in fruiting stage is 5.5-6.5.
Pleurotus eryngii is a kind of decompose fiber rope, xylogen, edible mushrooms that protein ability is stronger, Carbon and nitrogen sources in the raw material of various agricultural byproducts tankage and cultivating white mushroom can absorb, especially with major ingredients such as cotton seed hulls, waste cotton, hardwood sawdust, corn cobs, add in right amount the auxiliary materials such as wheat bran, rice bran, Semen Maydis powder and improve nitrogenous source for best.Because within the specific limits, Pleurotus eryngii nitrogenous source is abundanter, and mycelial growth is better, and output is higher.
In recent years, Pleurotus eryngii was the rising star in factory edible fungi family of China, because its quality is tender and crisp, had one light almond flavor, applicable fresh-keeping, and processing and the cooking are very popular.Add the rapid increasing of China's batch production yield of flammulina velutipes, impel the output of Pleurotus eryngii also within 2 years, to increase rapidly at this.
About cultivation matrix and the method for Pleurotus eryngii, below patent done disclosure:
CN 101456772 B " culture material of a kind of Pleurotus eryngii and preparation method thereof " disclose a kind of culture material and preparation method thereof, culture material comprises cotton seed hulls or wheat straw or straw or corn cob 75-80%, wheat bran or rice bran 5-12%, just 8-10% of the argol of poultry, Semen Maydis powder 3-8%, plaster of Paris 1-2%, sucrose 0.5-1.5%, unslaked lime 0.5-1.5%, add potassium primary phosphate 0.1-0.3% by the total amount of above-mentioned batching again, magnesium sulfate 0.1-0.2%, each component is all weight percent, the argol of cotton seed hulls or wheat straw or straw or corn cob and poultry just uses the long-pending retting of the windrow that advances: the culture material after Xinbao mushroom culturing is also field earth backing processing on the spot: adopt this technical scheme, can make Pleurotus geesteranus biological growth better, overcome and in prior art, do not had specially for the grow defect of the culture material of preparing of Pleurotus geesteranus, simplify production technique, shorten the growth cycle of Pleurotus eryngii, improve the biological transformation ratio of Pleurotus eryngii, but its shortcoming is that not only raw material is many for the formula of culture material, production cost is higher, and batching is complicated, production environment peculiar smell weight, body and mind impact on staff is larger.
The patent of CN1166272C has disclosed " utilizing the method for corn stalk Xinbao mushroom culturing ", the method comprises that Pleurotus eryngii is the one-level kind of bacterial classification, the preparation of secondary kind, cultivation matrix preparation, under aseptic condition, by one of cultivation matrix access secondary kind, send out bacterium and cultivate, fruiting, adopt mushroom, it is characterized in that cultivation medium formula is: major ingredient: corn stalk 40-100 part, be weight part, lower same, cotton seed hulls 0-100 part; Auxiliary material: wheat bran 4-20 part, cottonseed cake 2-10 part, white sugar 0.4-2 part, terra alba 0.4-2 part, calcium superphosphate 0.4-2 part; Moisture: water 85-430 part, heavy 0.75-1.2 kilogram every bag of cultivation matrix pack, secondary kind inoculum size is every bag of 15-30 wheat, after inoculation, sack is tightened, sent out bacterium and cultivate 40-50 days, fruiting, former base forms rear 5-12 days and gathers, and can go out second batch of mushroom by change of tide after adopting mushroom again.Above-mentioned method Xinbao mushroom culturing quality is slightly soft, and fruiting lacks of staying power.
CN101456772B discloses substratum of a kind of Pleurotus eryngii and preparation method thereof, and the method is specific as follows:
A kind of substratum of Pleurotus eryngii, this substratum comprises crop stalk 75~80%, wheat bran or rice bran 5~12%, the argol of poultry just 8~10%, Semen Maydis powder 3~8%, the plaster of Paris 1~2%, sucrose 0.5~1.5%, unslaked lime 0.5~1.5%, then add the potassium primary phosphate and 0.1~0.2% magnesium sulfate that account for above-mentioned batching total amount 0.1~0.3%, each component is all weight percent; Before described crop stalk uses, first water is fully moistening, piles up retting 5~7 days.In two kinds of above-mentioned methods taking corn stalk or crop stalk as major ingredient, and clearly disclosure in " Edible Fungi technology " the 3rd section Section eight that Chemical Industry Press publishes: Pleurotus eryngii is not liked and is produced in straw matrix, first-selected cotton seed hulls raw material in production, secondly can the neither too hard, nor too soft wood chip material of more alternative materials, as poplar, willow, elm, oak, toothed oak etc.
CN101717109 " a kind of pleurotus eryngii cultivating material " discloses following content: a kind of pleurotus eryngii cultivating material, it is characterized in that comprising the component of following weight percent: wood chip 35%, corn cob 30%, rice bran 20%, wheat bran 14%, Wingdale %, wherein corn cob comprises 4-5 object 60-80%, 5-10 object 20-40%.The preparation method of above-mentioned culture material comprises following step: wood chip, corn cob, rice bran, wheat bran, lime are mixed, and the stirring that adds water, makes water content reach a 55-67%.Above-mentioned method has mainly been done patent description to the particle diameter of corn cob, and this culture material ventilation property is relatively good, but nutrition is relatively dull, and the output in pleurotus eryngii fruiting later stage is had a certain impact.
Therefore, need to improve for above-mentioned defect, seek a kind of convenient batching, cost is low, nutritive ingredient is abundant, the method that cultivating rate is high.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of culture material that can make Pleurotus eryngii output improve and can reasonably utilize again the Pleurotus eryngii containing rubber tree wood chip of waste;
The present invention also provides the making method of above-mentioned culture material.
The present invention realizes by following technical scheme:
A making method that contains the culture material of the Pleurotus eryngii of rubber tree wood chip, comprises following step:
Corn cob 56.37 wheat bran 19.28
Rubber tree wood chip 21.47 terra albas 0.39
Lime 0.91 cotton seed hulls 5.43
Sugar 1.08 light calcium carbonates 0.94
The bacterium chaff 4.31 golden mushroom root bases of a fruit 7.46
Palm hull 3.25 soybean meal 2.07
Cold press peanut meal 3.56 urea 0.35
Manganese 0.15 bronze medal 0.07;
Above-mentioned sugar is at least one in glucose, fructose, maltose and sucrose;
Above-mentioned bacterium chaff is the waste material of cultivating needle mushroom or mushroom;
Waste material, sawdust or wood shavings powder etc. that described rubber tree wood chip stays while being rubber tree pruning or wood working;
The above-mentioned golden mushroom root base of a fruit is after needle mushroom is gathered, and packaging needed the root base of a fruit of the connection culture material part of excision in the past.
Above-mentioned palm hull is in plam oil refine process, to remove the fruit stone housing parts peeling off after pulp, and it strips off from palm, pure natural plants integral part.
Its water ratio of above-mentioned soybean meal is 4-6%;
The water ratio of described cold press peanut meal is 5-7%;
The water content of above-mentioned rubber tree wood chip is 6-8%.
Preferably, above-mentioned sugar is the mixture of glucose and fructose, and the weight ratio of glucose and fructose is 1:3.
The making method of the culture material of the Pleurotus eryngii of above-mentioned rubber tree wood chip of the present invention, comprises following step:
(1) prepare culture material by following parts by weight:
Corn cob 56.37 wheat bran 19.28
Rubber tree wood chip 21.47 terra albas 0.39
Lime 0.91 cotton seed hulls 5.43
Sugar 1.08 light calcium carbonates 0.94
The bacterium chaff 4.31 golden mushroom root bases of a fruit 7.46
Palm hull 3.25 soybean meal 2.07
Cold press peanut meal 3.56 urea 0.35
Manganese 0.15 bronze medal 0.07;
Above-mentioned sugar is at least one in glucose, fructose, maltose and sucrose;
Above-mentioned bacterium chaff is the waste material of cultivating needle mushroom or mushroom;
Described rubber tree wood chip is the rubber tree waste material pruning or stay when wood working, sawdust, wood shavings powder etc.
The above-mentioned golden mushroom root base of a fruit is after needle mushroom is gathered, and packaging needed the part root base of a fruit of the connection culture material of excision in the past.
Above-mentioned palm hull is in plam oil refine process, to remove the fruit stone housing parts peeling off after pulp.
Its water ratio of above-mentioned soybean meal is 4-6%, and the water ratio of cold press peanut meal is 5-7%;
Above-mentioned corn cob is to be crushed to the material that particle diameter is 2-5mm;
The water content that above-mentioned rubber tree wood chip is is 6-8%;
(2) corn cob, rubber tree wood chip, cotton seed hulls, palm hull are added water and fully soak, the add-on of described water is 1-2 times of above-mentioned compound weight, keeps 6-8 hour; Lime, terra alba, light calcium carbonate, urea, manganese, copper, sugar is soluble in water, stir, obtain mixing solutions, then in the solution of gained, add wheat bran, the golden mushroom root base of a fruit, bacterium chaff, soybean meal, cold press peanut meal; Then with the raw material blending having infiltrated before.
(3) by above-mentioned 2) in the mixture that mixes, pack (bottle) according to a conventional method, 120~140 DEG C of high-temperature sterilization 2.0~2.5h, are pleurotus eryngii cultivating material.
Wherein the treatment process of rubber tree wood chip is, rubber tree wood chip dries to water content 10-15% after collecting, and is crushed to particle diameter 1-5mm;
Wherein the treatment process of palm hull is, palm hull is shakeout and dried to water content 10-12% after collecting, and is crushed to particle diameter 2-10mm;
Wherein the treatment process of the golden mushroom root base of a fruit is, after the golden mushroom root base of a fruit is sheared, oven dry to its moisture content is 5-10%, is crushed to below particle diameter 1-3mm;
Preferably, the water ratio of above-mentioned culture material is 65-70%.
As a modification of the present invention, as follows containing the making method of the culture material of the Pleurotus eryngii of brown rubber tree wood chip:
(1) prepare culture material by following parts by weight:
Corn cob 56.37 wheat bran 19.28
Rubber tree wood chip 14.47 palm leaves 9.56
Terra alba 0.39 lime 0.91
Cotton seed hulls 5.43 sugar 1.08
Light calcium carbonate 0.94 bacterium chaff 4.31
The golden mushroom root base of a fruit 7.46 palm hull 3.25
Soybean meal 2.07 cold press peanut meals 3.56
Urea 0.35 manganese 0.15
Copper 0.07;
Above-mentioned sugar is at least one in glucose, fructose, maltose and sucrose;
Bacterium chaff is the waste material of cultivating needle mushroom or mushroom;
The treatment process of rubber tree wood chip is that rubber tree wood chip is dried to water content below 15% after collecting, and is crushed to particle diameter 1-5mm;
The treatment process of the golden mushroom root base of a fruit is, dry to moisture content be 5-10%, be crushed to below particle diameter 1-3mm;
Its water ratio of soybean meal is 4-6%, and the water ratio of described cold press peanut meal is 5-7%;
Corn cob is to be crushed to the material that particle diameter is 0.2-0.5cm;
The water content that rubber tree wood chip is is 5-7%;
The treatment process of palm leaves is by shredding the order to 20-30 after palm leaves collection, to add bubble wet, accumulation 2-4 hour, the 1-2 that described amount of water is palm leaves times;
(2) corn cob, rubber tree wood chip, cotton seed hulls, palm leaves, palm hull are added water and fully soak, the add-on of described water is 1-2 times of above-mentioned compound weight, keeps 6-8 hour; Lime, terra alba, light calcium carbonate, urea, manganese, copper, sugar is soluble in water, stir, obtain mixing solutions, then in the solution of gained, add wheat bran, the golden mushroom root base of a fruit, bacterium chaff, soybean meal, cold press peanut meal; Then with the raw material blending having infiltrated before.
(3) by above-mentioned 2) in the mixture that mixes, pack (bottle) according to a conventional method, 120~140 DEG C of high-temperature sterilization 2.0~2.5h, obtain pleurotus eryngii cultivating material.
Formula taking corn cob as major ingredient, the sporophore growth cycle is short, output is also higher, this is relevant to corn cob physicochemical property, corn cob granule degree is larger, air permeability is good, though water suction is slow simultaneously, but water regain is large, water retention capacity is strong, low price, be suitable as factory culture major ingredient, but while using corn cob, granularity can not be too large, the corn cob hardness that granularity is larger is larger, should not be degraded by mycelia, and the large corn cob of granularity, if the time of prewetting falls short of, when sterilizing, center is drier, do not reach required sterilising temp, easily cause sterilizing not thorough, cause environmental pollution.
Formula taking cotton seed hulls as major ingredient, fruiting number is many, and single mushroom is heavy on the low side, thereby has affected output, and growth cycle is also longer, therefore should not add more.
Formula taking wood chip as major ingredient, mushroom body color is whiter, but output is very low, wood chip cost is minimum, but needs the accumulation of long period to send out this locality, and composition is unstable, can cause the underproduction if contain hazardous substance, therefore, not all wood chip is all suitable for Xinbao mushroom culturing.
Rubber tree is a kind of aiphyllium of Euphorbiaceae, originates in the tropical rain forest of Brazilian Amazon Basin, up to 30 meters.Because its resin is the main raw material of natural rubber, some state's daives life of a family of torrid areas, the world are introduced a fine variety now, especially the widest with the cultivation of South Asia various countries.All introducing culture in a large number of China Hainan Island, Taiwan and our province southern areas.
Manganese can promote mycelial growth, improve fruiting body yield, and medium and small grade sporophore size and number is had a certain impact, the mechanism of action of manganese forms relevant with the impact of superoxide activity with manganese on Yeast Nucleic Acid, copper is to some bacterial strain, can strengthen its resistance against diseases, but excessive concentration can affect output.
Rubber tree wood chip is compared with common wood chip, be applied in the cultivation of Pleurotus eryngii, its advantage is that rubber tree wood chip, except containing a large amount of Mierocrystalline cellulose and xylogen, also contains the starch of 3-4% and 1.2% protein, its nutrition is more nutritious than common fast-growing hardwood sawdust, be more suitable for that hypha of edible fungus grows and fruiting, and rubber tree material structure is even, belongs to diffuse-porous wood, water-absorbent is strong, good water-retaining property.
Beneficial effect of the present invention is, adopts substrate culture Pleurotus eryngii of the present invention, and its mycelial growth is vigorous, and mycelia is pure white dense, there will not be the situations such as contaminated bacteria bag.Pleurotus eryngii sporophore stem is pure white, and mushroom type rounding, does not have misshapen mushroom, and biological transformation ratio has reached 108%.In addition, because fast development and the broad-leaf forest resource of mushroom industry are increasingly exhausted, need to try to explore to substitute other resource of wood chip.Rubber tree is as a kind of economic tree; need constantly to update; about 30 years economical lifves of gum; then will cut down renewal; the exploitation of rubber tree wood chip can effectively reduce the dependence of planting almond abalone mushroom raw material to conventional forest wood chip; to protecting national resource, the felling, the adjusting eubiosis etc. that reduce forest all play good action.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
Embodiment 1
The making method that contains the culture material of the Pleurotus eryngii of rubber tree wood chip, comprises following step:
(1) prepare culture material by following parts by weight:
Corn cob 56.37 wheat bran 19.28
Rubber tree wood chip 21.47 terra albas 0.39
Lime 0.91 cotton seed hulls 5.43
Sugar 1.08 light calcium carbonates 0.94
The bacterium chaff 4.31 golden mushroom root bases of a fruit 7.46
Palm hull 3.25 soybean meal 2.07
Cold press peanut meal 3.56 urea 0.35
Manganese 0.15 bronze medal 0.07;
Above-mentioned sugar is glucose;
Bacterium chaff is the waste material of cultivating needle mushroom;
Palm hull treatment process is, shakeouts and dry to moisture content 10% after collection, is crushed to particle diameter 7mm.
The treatment process of the golden mushroom root base of a fruit is, by the golden mushroom root base of a fruit shear then dry to its moisture content be 8%, be crushed to below particle diameter 2mm;
Its water ratio of soybean meal is 5%, and the water ratio of described cold press peanut meal is 6%;
Corn cob is to be crushed to the material that particle diameter is 0.4cm;
The water content that rubber tree wood chip is is 7%;
(2) corn cob, rubber tree wood chip, cotton seed hulls, palm hull are added water and fully soak, the add-on of described water is 1.5-2 times of above-mentioned compound weight, keeps 8 hours; Lime, terra alba, light calcium carbonate, urea, manganese, copper, sugar is soluble in water, stir, obtain mixing solutions, then in the solution of gained, add wheat bran, the golden mushroom root base of a fruit, bacterium chaff, soybean meal, cold press peanut meal; Then with the raw material blending having infiltrated before.
(3) by above-mentioned 2) in the mixture that mixes, according to a conventional method culture material is packed into 17 × 40 × 0.05cm polypropylene plastics pocket, 125 DEG C of high-temperature sterilization 2.2h, are pleurotus eryngii cultivating material; Inoculation, a bacterium and management of producing mushroom process routinely technical requirements are carried out.
Comparative example 1
Be with the difference of embodiment 1, the wood chip adopting in comparative example 1 is willow wood chip; All the other are identical with embodiment 1;
The culture material that the method for embodiment 1 and comparative example 1 is made, on above-mentioned two kinds different culture materials, the production index of Pleurotus eryngii is as follows:
As can be seen from the results, the Pleurotus eryngii mycelia growing way of embodiment 1 is good, sends out bacterium fast, and output is high, and cost is low, and economic benefit environmental benefit is good, is worthy to be popularized.
Embodiment 2
The making method that contains the culture material of the Pleurotus eryngii of palm tree wood chip, comprises following step:
(1) prepare culture material by following parts by weight:
Corn cob 56.37 wheat bran 19.28
Rubber tree wood chip 14.47 palm leaves 9.56
Terra alba 0.39 lime 0.91
Cotton seed hulls 5.43 sugar 1.08
Light calcium carbonate 0.94 bacterium chaff 4.31
The golden mushroom root base of a fruit 7.46 palm hull 3.25
Soybean meal 2.07 cold press peanut meals 3.56
Urea 0.35 manganese 0.15
Copper 0.07;
Above-mentioned sugar is the mixture of glucose and fructose 1:1;
Bacterium chaff is the waste material of cultivating needle mushroom or mushroom;
The treatment process of the golden mushroom root base of a fruit is, dry to moisture content be 8%, be crushed to below particle diameter 2mm;
Palm hull treatment process is, shakeouts and dry to moisture content 10% after collection, is crushed to particle diameter 8mm;
Its water ratio of soybean meal is 5%, and the water ratio of described cold press peanut meal is 6%;
Corn cob is to be crushed to the material that particle diameter is 0.3-0.5cm;
The water content that rubber tree wood chip is is 6%;
The treatment process of palm leaves is, after will palm leaves collecting, shred the order to 20-30, adds bubble wet, piles up the 1.5-2 that described amount of water is palm leaves times 4 hours;
(2) corn cob, rubber tree wood chip, cotton seed hulls, palm leaves, palm hull are added water and fully soak, the add-on of described water is 1.5-2 times of above-mentioned compound weight, keeps 8 hours; Lime, terra alba, light calcium carbonate, urea, manganese, copper, sugar is soluble in water, stir, obtain mixing solutions, then in the solution of gained, add wheat bran, the golden mushroom root base of a fruit, bacterium chaff, soybean meal, cold press peanut meal; Then with the raw material blending having infiltrated before.
(3) by above-mentioned 2) in the mixture that mixes, pack (bottle) according to a conventional method, 125 DEG C of high-temperature sterilization 2.2h, obtain pleurotus eryngii cultivating material.
It is fast that the present embodiment Pleurotus eryngii is sent out bacterium, grows vigorous, and mycelia is pure white dense, do not occur contaminated bacteria bag.Pleurotus eryngii sporophore mushroom type rounding, stem is pure white, there is no misshapen mushroom, fresh mushroom biological transformation ratio has reached 111%, and output exceeds 3% than embodiment 1, simultaneously, the consumption that the planting material of this embodiment has been avoided using common wood chip and obviously reduced cotton seed hulls, has effectively reduced the destruction of Deforestation to environment, in the situation that not reducing income, ecological benefits are obvious, are worthy to be popularized.
Embodiment 3
A making method that contains the culture material of the Pleurotus eryngii of rubber tree wood chip, comprises following step:
(1) prepare culture material by following parts by weight:
Corn cob 56.37 wheat bran 19.28
Rubber tree wood chip 21.47 terra albas 0.39
Lime 0.91 cotton seed hulls 5.43
Sugar 1.08 light calcium carbonates 0.94
The bacterium chaff 4.31 golden mushroom root bases of a fruit 7.46
Soybean meal 2.07 cold press peanut meals 3.56
Urea 0.35 manganese 0.15
Copper 0.07;
Above-mentioned sugar is glucose;
Bacterium chaff is the waste material of cultivating needle mushroom;
The treatment process of the golden mushroom root base of a fruit is, by the golden mushroom root base of a fruit shear then dry to its moisture content be 8%, be crushed to particle diameter 2-3mm;
Its water ratio of soybean meal is 5%, and the water ratio of described cold press peanut meal is 6%;
Corn cob is to be crushed to the material that particle diameter is 0.3-0.5cm;
The water content that rubber tree wood chip is is 7%;
(2) corn cob, rubber tree wood chip, cotton seed hulls, palm hull are added water and fully soak, the add-on of described water is 1.5-2 times of above-mentioned compound weight, keeps 8 hours; Lime, terra alba, light calcium carbonate, urea, manganese, copper, sugar is soluble in water, stir, obtain mixing solutions, then in the solution of gained, add wheat bran, the golden mushroom root base of a fruit, bacterium chaff, soybean meal, cold press peanut meal; Then with the raw material blending having infiltrated before.
(3) by above-mentioned 2) in the mixture that mixes, according to a conventional method culture material is packed into 17 × 40 × 0.05cm polypropylene plastics pocket, 125 DEG C of high-temperature sterilization 2.2h, are pleurotus eryngii cultivating material; Inoculation, a bacterium and management of producing mushroom process routinely technical requirements are carried out.
In palm hull, contain abundant mineral element, what wherein content was maximum is potassium element,, containing potassium 6-12%, more than 90% is wherein generally water-soluble, exists with carbonate form; Next is phosphorus, generally containing 1.5-3%; Also contain the micronutrient elements such as calcium, magnesium, silicon, sulphur and iron, manganese, copper, zinc, boron, molybdenum.
In the present embodiment, do not add palm hull, its effect is as follows: it is fast that Pleurotus eryngii mycelia sends out bacterium, and mycelia is pure white dense, does not occur contaminated bacteria bag.Pleurotus eryngii sporophore mushroom type rounding, stem is pure white, there is no misshapen mushroom, and biological transformation ratio is 98%.Compared with embodiment 1, mycelia growing way is slightly poor, and the fruiting later stage lacks of staying power, and output is lower by 10% than embodiment 1.

Claims (8)

1. the culture material that contains the Pleurotus eryngii of rubber tree wood chip, is characterized in that, described culture material comprises the composition of following portions by weight:
Corn cob 56.37 wheat bran 19.28
Rubber tree wood chip 21.47 terra albas 0.39
Lime 0.91 cotton seed hulls 5.43
Sugar 1.08 light calcium carbonates 0.94
The bacterium chaff 4.31 golden mushroom root bases of a fruit 7.46
Palm hull 3.25 soybean meal 2.07
Cold press peanut meal 3.56 urea 0.35
Manganese 0.15 bronze medal 0.07;
Above-mentioned sugar is at least one in glucose, fructose, maltose and sucrose;
Described bacterium chaff is the waste material of cultivating needle mushroom or mushroom;
Waste material, sawdust or wood shavings powder that described rubber tree wood chip stays while being rubber tree pruning or wood working;
The described golden mushroom root base of a fruit is after needle mushroom is gathered, the root base of a fruit of the connection culture material part of excision before packaging;
Described palm hull is the fruit stone housing parts peeling off remove pulp in the time extracting plam oil after;
Its water ratio of described soybean meal is 4-6%, and the water ratio of described cold press peanut meal is 5-7%;
The water content of described rubber tree wood chip is 6-8%.
2. the culture material of the Pleurotus eryngii containing rubber tree wood chip as claimed in claim 1, is characterized in that, described sugar is the mixture of glucose and fructose, and the weight ratio of glucose and fructose is 1:3.
3. the making method of the culture material of the Pleurotus eryngii containing rubber tree wood chip as claimed in claim 1 or 2, comprises following step:
(1) prepare culture material by following parts by weight:
Corn cob 56.37 wheat bran 19.28
Rubber tree wood chip 21.47 terra albas 0.39
Lime 0.91 cotton seed hulls 5.43
Sugar 1.08 light calcium carbonates 0.94
The bacterium chaff 4.31 golden mushroom root bases of a fruit 7.46
Palm hull 3.25 soybean meal 2.07
Cold press peanut meal 3.56 urea 0.35
Manganese 0.15 bronze medal 0.07;
Above-mentioned sugar is at least one in glucose, fructose, maltose and sucrose;
Described bacterium chaff is the waste material of cultivating needle mushroom or mushroom;
Waste material, sawdust or wood shavings powder that described rubber tree wood chip stays while being rubber tree pruning or wood working;
The described golden mushroom root base of a fruit is after needle mushroom is gathered, the root base of a fruit of the connection culture material part of excision before packaging;
Described palm hull is the fruit stone housing parts peeling off remove pulp in the time extracting plam oil after;
Its water ratio of described soybean meal is 4-6%;
The water ratio of described cold press peanut meal is 5-7%;
Described corn cob is to be crushed to the material that particle diameter is 2-5mm;
The water content that described rubber tree wood chip is is 6-8%;
(2) corn cob, rubber tree wood chip, cotton seed hulls, palm hull are added water and fully soak, the add-on of described water is 1-2 times of above-mentioned compound weight, keeps 6-8 hour; Lime, terra alba, light calcium carbonate, urea, manganese, copper, sugar is soluble in water, stir, obtain mixing solutions, then in the solution of gained, add wheat bran, the golden mushroom root base of a fruit, bacterium chaff, soybean meal, cold press peanut meal; Then with the raw material blending having infiltrated before;
(3) by the mixture mixing in above-mentioned steps (2), pack or bottling according to a conventional method, 120~140 DEG C of high-temperature sterilization 2.0~2.5h, are pleurotus eryngii cultivating material.
4. the making method of the culture material of the Pleurotus eryngii containing rubber tree wood chip as claimed in claim 3, is characterized in that, rubber tree wood chip dries to its water content 10-15%, is crushed to particle diameter 1-5mm.
5. the making method of the culture material of the Pleurotus eryngii containing rubber tree wood chip as claimed in claim 3, is characterized in that, described palm hull is shakeout and dried to water content 10-12% after collecting, and is crushed to particle diameter 2-10mm.
6. the treatment process of the golden mushroom root base of a fruit as claimed in claim 3 is, the golden mushroom root base of a fruit is sheared, and oven dry to its moisture content is 5-10%, is crushed to below particle diameter 1-3mm.
7. the making method of the culture material of the Pleurotus eryngii containing rubber tree wood chip as claimed in claim 3, is characterized in that, the water ratio of described culture material is 65-70%.
8. the making method of the culture material of the Pleurotus eryngii containing brown rubber tree wood chip as claimed in claim 1 or 2, comprises following step:
(1) prepare culture material by following parts by weight:
Corn cob 56.37 wheat bran 19.28
Rubber tree wood chip 14.47 palm leaves 9.56
Terra alba 0.39 lime 0.91
Cotton seed hulls 5.43 sugar 1.08
Light calcium carbonate 0.94 bacterium chaff 4.31
The golden mushroom root base of a fruit 7.46 palm hull 3.25
Soybean meal 2.07 cold press peanut meals 3.56
Urea 0.35 manganese 0.15
Copper 0.07;
Above-mentioned sugar is at least one in glucose, fructose, maltose and sucrose;
Above-mentioned bacterium chaff is the waste material of cultivating needle mushroom or mushroom;
The treatment process of the above-mentioned golden mushroom root base of a fruit is, dry to moisture content be 5-10%, be crushed to below particle diameter 1-3mm;
Its water ratio of above-mentioned soybean meal is 4-6%, and the water ratio of described cold press peanut meal is 5-7%;
Above-mentioned corn cob is to be crushed to the material that particle diameter is 0.2-0.5cm;
The water content that above-mentioned rubber tree wood chip is is 6-8%;
The treatment process of above-mentioned palm leaves is by shredding the order to 20-30 after palm leaves collection, to add bubble wet, accumulation 2-4 hour, the 1-2 that described amount of water is palm leaves times;
(2) corn cob, rubber tree wood chip, cotton seed hulls, palm leaves, palm hull are added water and fully soak, the add-on of described water is 1-2 times of above-mentioned compound weight, keeps 6-8 hour; Lime, terra alba, light calcium carbonate, urea, manganese, copper, sugar is soluble in water, stir, obtain mixing solutions, then in the solution of gained, add wheat bran, the golden mushroom root base of a fruit, bacterium chaff, soybean meal, cold press peanut meal; Then with the raw material blending having infiltrated before;
(3) by the mixture mixing in above-mentioned steps (2), pack or bottling according to a conventional method, at 120~140 DEG C, high-temperature sterilization 2.0~2.5h, obtains pleurotus eryngii cultivating material.
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CN104163705A (en) * 2014-07-28 2014-11-26 安庆市绿茵农业发展有限公司 Pleurotus eryngii culture medium with mushroom dregs as raw material and preparation method thereof
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CN105237159A (en) * 2015-09-10 2016-01-13 凤台县绿然农业发展有限公司 High-effective pleurotus eryngii culture medium mainly prepared from roots and bases of flammulina velutipes and preparation method thereof
CN105254400A (en) * 2015-09-23 2016-01-20 山东省农业科学院农业资源与环境研究所 Domestic fungus culture material prepared from Chinese wistaria byproducts, and preparation method thereof
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CN105152803A (en) * 2015-10-21 2015-12-16 济南舜昊生物科技有限公司 Pleurotus eryngii culture medium and preparation method thereof
CN105218257A (en) * 2015-11-12 2016-01-06 山东省农业科学院农业资源与环境研究所 A kind of pleurotus eryngii cultivating material suppressing miscellaneous bacteria and preparation method thereof
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CN114847079A (en) * 2022-05-17 2022-08-05 福建省农业科学院亚热带农业研究所(福建省农业科学院蔗麻研究中心) Application of fructus cannabis sativa seeds in edible fungus cultivation
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