CN103891505B - A kind of authentication method of tea tree impoverishment tolerant resource - Google Patents
A kind of authentication method of tea tree impoverishment tolerant resource Download PDFInfo
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- CN103891505B CN103891505B CN201410118557.5A CN201410118557A CN103891505B CN 103891505 B CN103891505 B CN 103891505B CN 201410118557 A CN201410118557 A CN 201410118557A CN 103891505 B CN103891505 B CN 103891505B
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Abstract
An authentication method for tea tree impoverishment tolerant resource, belongs to technical field of tea tree cultivation.It is characterized in that comprising the following steps: 1) contrast tea tree breed and select; 2) tea tree branch is selected to prepare with cuttings; 3) plug media prepares; 4) cuttings cuttage; 5) in greenhouse, overlay film is taken root; 6) management of cuttage seeding; 7) Resources of Tea Plant impoverishment tolerant qualification.The authentication method of above-mentioned a kind of tea tree impoverishment tolerant resource, in greenhouse, Resources of Tea Plant is carried out cave dish cuttage and is cultivated a period of time, then according to the biomass of tea shoot, carry out characterization and evaluation to the impoverishment tolerant of Resources of Tea Plant, this authentication method is simple to operate, and qualification result is reliable.
Description
Technical field
The invention belongs to technical field of tea tree cultivation, be specially a kind of authentication method of tea tree impoverishment tolerant resource.
Background technology
Nitrogen is the most important essential nutrient element improving tea yield and kind.Nitrogen content in tea tree, especially young sprout nitrogen content and tea leaf quality closely related.In recent decades, tea place fertilising is based on ammonium nitrogen.But after tea tree absorbs ammonium nitrogen, root system can discharge a large amount of H+, thus, cause tea garden soil acidifying rapidly.According to statistics, the nitrate nitrogen existed in soil is most of between 50-150mg/kg, far above ammonium nitrogen.Consider that nitrate nitrogen is topmost inorganic nitrogen form in tea garden soil, therefore except ammonium nitrogen, nitrate nitrogen is also the important nitrogen source of tea tree.As can be seen here, select tea tree impoverishment tolerant kind, increase tea tree to NO3--N translocation availability, to the amount of application reducing tea place ammonium nitrogen, slow down tea garden soil acidifying, reducing tea place fertilizers input has important directive significance.At present, about the authentication method of tea tree impoverishment tolerant resource is still blank.
Summary of the invention
For the above-mentioned problems in the prior art, the object of the invention is to design the technical scheme of the authentication method that a kind of tea tree impoverishment tolerant resource is provided, in greenhouse, Resources of Tea Plant is carried out cave dish cuttage and is cultivated a period of time, then according to the biomass of tea shoot, characterization and evaluation is carried out to the impoverishment tolerant of Resources of Tea Plant.
The authentication method of described a kind of tea tree impoverishment tolerant resource, is characterized in that comprising the following steps:
1) contrast tea tree breed to select: select Fuding white tea as the contrast of impoverishment tolerant tea tree breed, Dragon Well tea 43 is as resistance to fertile tea tree check variety;
2) tea tree branch is selected to prepare with cuttings: tea tree is not adopted spring tea, kind to be identified and Fuding white tea, Dragon Well tea 43 carry out fringe bar respectively and stay foster, to by the end of June at the beginning of 7 months, after branch bar of receiving guest with tea is hardening, by stem section still for green tea tree branch is cut, then, branch is cut into the long cuttings of the 2.5-3.5cm of bud one leaf;
3) plug media prepares: adopt cave dish to carry out cuttage, Pin Shi Top of Denmark peat using volume ratio 1-3:0.5-1.5:0.5-1.5: perlite: vermiculite mixture is as cutting medium, cutting medium water semi-transparent after sabot again, during the dish of dress cave, require the compacting flattening a little of dress plug media, ensure that every hole tightness is basically identical, after assembling, irrigate with water, finally water execute sterilization with the liquor potassic permanganate of 5 ‰, preparation at least completes in cuttage the previous day;
4) cuttings cuttage: select overcast and rainy, by the cuttings cuttage that shears in plug media, compresses matrix, and adds cutting medium in matrix hole on the low side, then sprays carbendazim solution sterilization;
5) in greenhouse, overlay film is taken root: after cuttage, builds the Small plastic shed that 50-60cm is high, then covers brand-new plastic film, and surrounding is sealed, and opened by the sunshade net in greenhouse, temperature controls, at 15 DEG C-38 DEG C, to carry out taking root of tea shoot;
6) management of cuttage seeding: after treating that cuttings major part is taken root, selects overcast and rainyly to take film off, and the nitrogen-phosphorus-potassium compound fertilizer solution of blade spraying 1-1.5 ‰, afterwards, water with the nitrogen-phosphorus-potassium compound fertilizer solution of 2-2.5 ‰, every two weeks are once;
7) Resources of Tea Plant impoverishment tolerant qualification: cuttage is after 12 months, measure the tea shoot plant height of kind to be identified and check variety, stem slightly, the number of sheets; Tea shoot plant height, Resources of Tea Plant that the number of sheets is all better than Fuding white tea, to be accredited as impoverishment tolerant strong; Tea shoot plant height, stem thick and the be accredited as impoverishment tolerant of number of sheets indices all lower than Dragon Well tea 43 weak.
The authentication method of described a kind of tea tree impoverishment tolerant resource, is characterized in that in step 3): Pin Shi Top of Denmark peat: perlite: vermiculite volume ratio is 2:1:1.
The authentication method of described a kind of tea tree impoverishment tolerant resource, is characterized in that in step 5): temperature of taking root is 15 DEG C-38 DEG C.
The authentication method of above-mentioned a kind of tea tree impoverishment tolerant resource, in greenhouse, Resources of Tea Plant is carried out cave dish cuttage and is cultivated a period of time, then according to the biomass of tea shoot, carry out characterization and evaluation to the impoverishment tolerant of Resources of Tea Plant, this authentication method is simple to operate, and qualification result is reliable.
Embodiment
Now in conjunction with embodiments of the invention, further illustrate beneficial effect of the present invention.
Embodiment 1:
1) contrast tea tree breed to select: select Fuding white tea as the contrast of impoverishment tolerant tea tree breed, Dragon Well tea 43 is as resistance to fertile tea tree check variety, and black ox is early kind to be identified;
2) cuttings obtains: the tea tree of black ox morning, Fuding white tea, Dragon Well tea 43 does not adopt spring tea, carrying out fringe bar stays foster, to by the end of June to the cloudy day at the beginning of 7 months, by stem section still for the green hard branch of tea tree is cut, then, branch is cut into the long cuttings of the 2.5-3.5cm of bud one leaf;
3) plug media prepares: adopt the cave of 50 hole depth 4cm to coil and carry out cuttage, Pin Shi Top of Denmark peat using volume ratio 1-3:0.5-1.5:0.5-1.5: perlite: vermiculite mixture is as cutting medium, cutting medium water semi-transparent after sabot again, during the dish of dress cave, require the compacting flattening a little of dress plug media, ensure that every hole tightness is basically identical, after assembling, irrigate with water, finally water execute sterilization with the liquor potassic permanganate of 5 ‰, preparation at least completes in cuttage the previous day;
4) cuttings cuttage: select overcast and rainy, by the cuttings cuttage that shears in plug media, compresses matrix, and adds cutting medium in matrix hole on the low side, then sprays carbendazim solution sterilization;
5) in greenhouse, overlay film is taken root: after cuttage, builds the Small plastic shed that 50-60cm is high, then covers brand-new plastic film, and surrounding is sealed, and opened by the sunshade net in greenhouse, temperature controls, at 15 DEG C-38 DEG C, to carry out taking root of tea shoot;
6) management of cuttage seeding: cuttage is after 2 months, and most of cuttings is all taken root, and takes film off overcast and rainy, and the nitrogen-phosphorus-potassium compound fertilizer solution of blade spraying 1 ‰, afterwards, with the nitrogen-phosphorus-potassium compound fertilizer solution pouring of 2.5 ‰, every two weeks once, notes carrying out the extermination of disease and insect pest simultaneously;
7) Resources of Tea Plant impoverishment tolerant qualification: cuttage is after 12 months, measure black ox early and the tea shoot plant height of check variety Fuding white tea and Dragon Well tea 43, stem thick, the number of sheets; Investigation finds, the plant height of wuniu early tea seedling, the number of sheets and Fuding white tea quite, and every growth indexes is better than Dragon Well tea 43; Therefore, black ox impoverishment tolerant is early accredited as by force.
Cuttage is after 1 year as can be seen from Table 1, and tea tree breed crow ox to be identified growth parameter(s) index early is obviously better than the weak check variety Dragon Well tea 43 of impoverishment tolerant, suitable with the check variety Fuding white tea that impoverishment tolerant is strong.Therefore, black ox impoverishment tolerant is early accredited as by force.
Claims (1)
1. an authentication method for tea tree impoverishment tolerant resource, is characterized in that comprising the following steps:
1) contrast tea tree breed to select: select Fuding white tea as the contrast of impoverishment tolerant tea tree breed, Dragon Well tea 43 is as resistance to fertile tea tree check variety;
2) tea tree branch is selected to prepare with cuttings: tea tree is not adopted spring tea, kind to be identified and Fuding white tea, Dragon Well tea 43 carry out fringe bar respectively and stay foster, to by the end of June at the beginning of 7 months, after branch bar of receiving guest with tea is hardening, by stem section still for green tea tree branch is cut, then, branch is cut into the long cuttings of the 2.5-3.5cm of bud one leaf;
3) plug media prepares: adopt cave dish to carry out cuttage, Pin Shi Top of Denmark peat using volume ratio 3:0.5-1.5:0.5-1.5: perlite: vermiculite mixture is as cutting medium, cutting medium water semi-transparent after sabot again, during the dish of dress cave, require the compacting flattening a little of dress plug media, ensure that every hole tightness is basically identical, after assembling, irrigate with water, finally water execute sterilization with the liquor potassic permanganate of 5 ‰, preparation at least completes in cuttage the previous day;
4) cuttings cuttage: select overcast and rainy, by the cuttings cuttage that shears in plug media, compresses matrix, and adds cutting medium in matrix hole on the low side, then sprays carbendazim solution sterilization;
5) in greenhouse, overlay film is taken root: after cuttage, builds the Small plastic shed that 50-60cm is high, then covers brand-new plastic film, and surrounding is sealed, and opened by the sunshade net in greenhouse, temperature controls, at 15 DEG C-38 DEG C, to carry out taking root of tea shoot;
6) management of cuttage seeding: after treating that cuttings major part is taken root, selects overcast and rainyly to take film off, and the nitrogen-phosphorus-potassium compound fertilizer solution of blade spraying 1-1.5 ‰, afterwards, water with the nitrogen-phosphorus-potassium compound fertilizer solution of 2-2.5 ‰, every two weeks are once;
7) Resources of Tea Plant impoverishment tolerant qualification: cuttage is after 12 months, measure the tea shoot plant height of kind to be identified and check variety, stem slightly, the number of sheets; Tea shoot plant height, Resources of Tea Plant that the number of sheets is all better than Fuding white tea, to be accredited as impoverishment tolerant strong; Tea shoot plant height, stem thick and the be accredited as impoverishment tolerant of number of sheets indices all lower than Dragon Well tea 43 weak.
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| CN1742541A (en) * | 2005-09-28 | 2006-03-08 | 中国农业科学院茶叶研究所 | Factorial seedling-culturing method for tea tree |
| CN101569266A (en) * | 2009-06-08 | 2009-11-04 | 丽水市农业科学研究院 | Method for propagating tea plants |
| CN101755586A (en) * | 2009-09-28 | 2010-06-30 | 无锡市茶叶品种研究所有限公司 | Hole-tray sticking culture method of tea-tree seedlings |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1742541A (en) * | 2005-09-28 | 2006-03-08 | 中国农业科学院茶叶研究所 | Factorial seedling-culturing method for tea tree |
| CN101569266A (en) * | 2009-06-08 | 2009-11-04 | 丽水市农业科学研究院 | Method for propagating tea plants |
| CN101755586A (en) * | 2009-09-28 | 2010-06-30 | 无锡市茶叶品种研究所有限公司 | Hole-tray sticking culture method of tea-tree seedlings |
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