CN103875609A - Cultural method for Oulenzia bakeri Hughes and application thereof - Google Patents

Cultural method for Oulenzia bakeri Hughes and application thereof Download PDF

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CN103875609A
CN103875609A CN201410143238.XA CN201410143238A CN103875609A CN 103875609 A CN103875609 A CN 103875609A CN 201410143238 A CN201410143238 A CN 201410143238A CN 103875609 A CN103875609 A CN 103875609A
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江宏斌
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Abstract

The invention provides a cultural method for Oulenzia bakeri Hughes and the application thereof. The method is characterized in that yeast or a material comprising a yeast component is used as compost for culturing the Oulenzia bakeri Hughes. The cultured Oulenzia bakeri Hughes can be used as fodder mites of predatory mites and used for culturing the predatory mites, the cultured predatory mites can be released on crops and woods to control harm of plant-feeding pest mites (or insects). The cultured Oulenzia bakeri Hughes mites can also be directly released on the crops and the woods to be used as the food source of the local predatory mites, and therefore the number of the predatory mites on the crops and the woods can be increased, and the control effect on the plant-feeding pest mites from the predatory mites (or the insects) can be improved. The harm to the crops and the woods from the plant-feeding pest mites (or the insects) can be reduced.

Description

Cultural method and the application thereof of Bei Shi Olympic mite
Technical field
The present invention relates to Bei Shi Olympic mite ( oulenzia bakerihughes) cultural method and application thereof.
Background technology
Bei Shi Olympic mite oulenzia bakerihughes is arachnids, Astigmata, and Wen Te mite section, a kind of mite class during Olympic mite belongs to, its synonym is Oulenzia arboricolaOudemans, Calvolia bakeri Hughes, calvolia arboricola(Oudemans) and lenzia arboricolaoudemans(Fan, et al. 2012; Hughes, 1962).Wherein Oulenzia, Calvolia and lenziaall refer to a genus in Zootaxy.Bei Shi Olympic mite inhabits (Fan, et al. 2012 on the blade and fruit of bamboo grows, jute and orange tree; Hughes, 1962; Muma, 1965; Buosi, 2006; Reinaldo, 2001).Think that in the world it is take plant leaf blade as food (Baker all the time, 1952), but just guess its feeding habits, do not carry out any biological study, more have no the forage mites using this mite as carnivorous mite class, any data that carnivorous mite class is cultivated.Because this mite does not also have Chinese name in China, therefore according to the custom of naming of Chinese, its Chinese name is decided to be Bei Shi Olympic mite by the inventor.The inventor, in the process that this mite is studied, has invented method and application thereof that the nucleus using yeast as composts or fertilisers of cultivating is cultivated Bei Shi Olympic mite.
Summary of the invention
One of task of the present invention provides the cultural method of Bei Shi Olympic mite.
Two of task of the present invention provides the application of Bei Shi Olympic mite.
The cultural method of Bei Shi Olympic mite of the present invention is realized with following technical scheme: the cultural method of Bei Shi Olympic mite of the present invention is using yeast or contains the material of zymasis as the composts or fertilisers of cultivating of Bei Shi Olympic mite, in order to cultivate Bei Shi Olympic mite.Yeast or can be separately in order to cultivate Bei Shi Olympic mite containing the material of zymasis, also can add other medium (as flour, wheat skin, vermiculite, wood chip, or their mixture) to form after mixture, then in order to cultivate Bei Shi Olympic mite.
The cultural method of Bei Shi Olympic mite of the present invention, its technological process is:
The first step: by the mixture of the yeast material of zymasis (or containing) or its and other medium put into one ventilative and can prevent culture vessel or the carrier of acarid escape;
Second step: then add more than 1 Bei Shi Olympic mite in culture vessel or carrier;
The 3rd step: will cultivate again condition of culture: temperature is 5-35 ℃, and relative air humidity is 50-100%RH, has or not illumination all can in culture vessel or carrier immigration culturing room; According to the quantity of the size of container, the Bei Shi Olympic mite that adds, and the difference of condition of culture, 10-100 days cultivated.
The application of Bei Shi Olympic mite of the present invention is: according to the cultural method of Bei Shi Olympic mite of the present invention, the Bei Shi Olympic mite of turning out can be used as the forage mites of carnivorous mite class, be applied to and cultivate carnivorous mite class, the concrete species of carnivorous mite class refer to Si Shi genus amblyseius berlese amblyseius( typhlodromips) swirskiiathias-Henriot, California genus amblyseius berlese neoseiulus( amblyseius) californicus(McGregor), pseudo-genus amblyseius berlese neoseiulus (Amblyseius) fallacies(Garman), the former genus amblyseius berlese in river amblyseiuseharaiamitai & Swirski, the nearly blind peaceful mite of lemon amblydromalus( amblyseius) limonicusgarman & McGregor, Anderson genus amblyseius berlese amblyseius andersonichant ,regression genus amblyseius berlese amblyseius( iphiseius) degeneransberlese, a kind of little blind peaceful mite typhlodromips( transeius) montdorensisthe very peaceful mite of Schicha, one euseius gallicuskreiter & Tixier .(illustrate: herein the title of carnivorous mite class in state-owned a lot of calls, but they all only have a kind of formal name used at school separately, and the little blind peaceful mite of above-mentioned one typhlodromips( transeius) montdorensisthe very peaceful mite of Schicha and one euseius gallicuskreiter & Tixier there is no Chinese name .)
Its application of cultural method of described Bei Shi Olympic mite, in the time using Bei Shi Olympic mite to cultivate above-mentioned carnivorous mite class as forage mites, can first in above-mentioned culture vessel or carrier, cultivate Bei Shi Olympic mite, when in culture vessel or carrier, when Bei Shi Olympic mite in every 0.1 gram of composts or fertilisers of cultivating reaches more than 1, add again carnivorous mite class, and the quantity of carnivorous mite class in every 0.1 gram of composts or fertilisers of cultivating is reached more than 1; Or, also can in described culture vessel or carrier, add Bei Shi Olympic mite and carnivorous mite class (its ratio is 1 carnivorous mite class of 1-100 Bei Shi Olympic mite ︰) simultaneously.The condition of culture of above-mentioned two kinds of training methods is identical with the 3rd step of the technological process of the cultural method of above-mentioned Bei Shi Olympic mite.
The application of Bei Shi Olympic mite of the present invention comprises: the Bei Shi Olympic mite of turning out, after packing, can be directly released in agriculture, woods crop, as the supplements of the carnivorous mite class in locality, to increase the food source of carnivorous mite class, thereby increase the population quantity of carnivorous mite class in agriculture, woods crop, closely strengthen the control action of carnivorous mite class to the phytophagy evil mite (or insect) on agriculture, woods crop, thereby reduce the harm of phytophagy evil mite (or insect) to agriculture, woods crop.
Compared with prior art, the cultural method of Bei Shi Olympic mite of the present invention and the novelty of application thereof are: there is no the biological any research data of Bei Shi Olympic mite both at home and abroad, more without any the related data of Bei Shi Olympic mite cultural method, the inventor be study in great detail after the biology of this mite, propose first using yeast or containing the material of zymasis the nucleus as the composts or fertilisers of cultivating of Bei Shi Olympic mite, and the condition of culture of Bei Shi Olympic mite is studied in detail, the condition of culture that proposes first Bei Shi Olympic mite is: temperature is 5-35 ℃, relative air humidity is 50-100%RH, have or not illumination all can.And Bei Shi Olympic mite is proposed in the world first can be used as the forage mites of above-mentioned carnivorous mite class, carry out the cultivation of above-mentioned carnivorous mite.The cultural method of Bei Shi Olympic mite of the present invention and the creativeness of application thereof are: think that in the world Bei Shi Olympic mite is take plant leaf blade as food (Baker all the time, 1952), and the inventor is after studying the biology of this mite, creatively invents using yeast or contained the material of zymasis as its composts or fertilisers of cultivating.The cultural method of Bei Shi Olympic mite of the present invention and the practicality of application thereof are: the Bei Shi Olympic mite of turning out can be used as the forage mites of carnivorous mite class, be used for cultivating carnivorous mite class, after the carnivorous mite class of turning out is packed, can be discharged on farm-forestry crop, play and control the object that evil mite, insect are caused harm, thereby reduce the use of agricultural chemicals, preserve the ecological environment and the mankind healthy; Or, the Bei Shi Olympic mite of turning out, also can be after packing, be directly released into the supplements as local carnivorous mite class in agriculture, woods crop, to increase the food source of carnivorous mite class, thereby increase the population quantity of carnivorous mite class on agriculture, woods crop, closely strengthen predation and the control action of carnivorous mite to phytophagy evil mite, insect, reduce phytophagy evil mite, the harm of insect to agriculture, woods crop.Conventional art uses the forage mites of storage mite class as carnivorous mite class; but there is the more data mite class (for example tyrophagus putrescentiae, oval baking soda mite, hesperidium mite) that shows to store in a warehouse can cause the allergy (being so-called acariasis) of human body skin and respiratory tract; and Bei Shi Olympic mite used in the present invention is from plant leaf blade; do not belong to storage mite class; can not cause the mankind's acariasis, can effectively protect that the producer and user's is healthy.In addition, the storage mite class that conventional art uses is from storage material, general all take vegetable material as food, so in the time of the carnivorous mite class using take storage mite class as forage mites cultivation, inevitably can storage mite class be discharged on agriculture, woods crop simultaneously, and plant is worked the mischief, for example tyrophagus putrescentiae (storage mite class) itself is exactly the insect of lepisphere stem crop, and although Bei Shi Olympic mite used in the present invention is from plant leaf blade, but they,, not take vegetable material as food, can not work the mischief to plant.Therefore the present invention has avoided two large disadvantages of conventional art, has significant progress with respect to conventional art.
 
Embodiment
Be to further illustrate of the present invention below in conjunction with specific embodiment and experimental data, but be not limitation of the present invention.
The cultural method of Bei Shi Olympic mite of the present invention, the concrete steps of an embodiment are:
The first step: the culture vessel and the lid thereof that are 1 liter by volume clean up, on lid, there is a ventilation mouth that accounts for lid area 1/3rd, ventilative cloth or paper sealing for ventilation mouth, cloth or paper must reach can breathe freely and can prevent the requirement that mite escapes, more particularly, generally printing paper can be used for the present invention.
Second step: 200 grams of vermiculites are put into above-mentioned culture vessel, and then add the yeast of 30 grams, and stir, yeast is dispersed in vermiculite.
The 3rd step: then add the Bei Shi Olympic mite of 10000 in above-mentioned culture vessel, the lid of covered container subsequently.
The 4th step: more above-mentioned culture vessel is moved in culturing room and cultivated, condition of culture: temperature is 25 ℃, relative air humidity is 90%RH.General cultivation can be used for 15-25 days.
The 5th step: when turning out after Bei Shi Olympic mite, add 10000 carnivorous mite classes in the container of above-mentioned cultivation Bei Shi Olympic mite, continue to cultivate under above-mentioned the same terms to obtain a large amount of carnivorous mite classes for 20 days.Or, also can repeat the above-mentioned first step and second step, the 3rd step changes the Bei Shi Olympic mite that adds 150000 in culture vessel into, and then add the carnivorous mite class of 5000, after the lid of covered container, move into after culturing room, under above-mentioned same culture conditions, cultivate 20 days, also can obtain a large amount of carnivorous mite classes.The carnivorous mite class of turning out, after packing, can be discharged on agriculture, woods crop, for the biological control of agriculture, woods crop evil mite, insect.
The 6th step: when the 4th step is turned out after a large amount of Bei Shi Olympic mites, also can directly pack, then be discharged on agriculture, woods crop, as the supplements of local carnivorous mite class on agriculture, woods crop, to increase the food source of carnivorous mite class, thereby increase the population quantity of carnivorous mite class on agriculture, woods crop, closely strengthen predation and the control action of carnivorous mite to phytophagy evil mite, insect, minimizing phytophagy evil mite, insect are caused harm to agriculture, woods crop.
Be below experimental datas more of the present invention, in order to prove feasibility of the present invention:
Table 1 Bei Shi Olympic mite growing take yeast as composts or fertilisers of cultivating (25 degree, 90%RH)
As seen from the data in Table 1, Bei Shi Olympic mite can normal development maturation during take yeast as composts or fertilisers of cultivating, but during merely take wheat skin as composts or fertilisers of cultivating, can not normal development, and all young mites are all dead within these few days.But add after yeast at Mai Pizhong, Bei Shi Olympic mite also can normal development, and close with the result of independent use yeast.Therefore in the time cultivating Bei Shi Olympic mite, in its composts or fertilisers of cultivating, yeast is necessary composition.
Table 2 Bei Shi Olympic mite take yeast as composts or fertilisers of cultivating fertility (25 degree, 90%RH)
As seen from the data in Table 2, can normal spawning breeding when Bei Shi Olympic mite is take yeast as composts or fertilisers of cultivating.
The different carnivorous mite classes of table 3 growing take Bei Shi Olympic mite as forage mites (25 degree, 90%RH)
Figure 201410143238X100002DEST_PATH_IMAGE005
As seen from the data in Table 3, above-mentioned carnivorous mite class can be grown by normal growth during as forage mites take Bei Shi Olympic mite.
The fertility (25 degree, 90%RH) of the different carnivorous mite classes of table 4 take Bei Shi Olympic mite as forage mites
Figure 193654DEST_PATH_IMAGE006
As seen from the data in Table 4, above-mentioned carnivorous mite class can normal spawning and breeding during as forage mites take Bei Shi Olympic mite.
The present invention is not limited to above-mentioned specific embodiment and experimental data, and other any or akin methods identical with the present invention that anyone draws under enlightenment of the present invention, within all dropping on protection scope of the present invention.

Claims (6)

1. Bei Shi Olympic mite oulenzia bakerithe cultural method of Hughes, is characterized in that: using yeast or containing the material of zymasis as the composts or fertilisers of cultivating of cultivating Bei Shi Olympic mite; Yeast or can be used alone containing the material of zymasis; the direct composts or fertilisers of cultivating as Bei Shi Olympic mite; or add other medium such as flour, wheat skin, vermiculite, wood chip to form after mixture by yeast or containing the material of zymasis, be re-used as the composts or fertilisers of cultivating of Bei Shi Olympic mite; The technological process of the cultural method of described Bei Shi Olympic mite is as follows:
The first step: by yeast or containing the material of zymasis, or its mixture forming with other medium such as flour, wheat skin, vermiculite, wood chip put into one ventilative and can prevent culture vessel or the carrier of acarid escape;
Second step: then add more than 1 Bei Shi Olympic mite in culture vessel or carrier;
The 3rd step: will cultivate again condition of culture: temperature is 5-35 ℃, and relative air humidity is 50-100%RH, has or not illumination all can in culture vessel or carrier immigration culturing room; According to the quantity of the size of container, the Bei Shi Olympic mite that adds, and the difference of condition of culture, 10-100 days cultivated.
2. the application of the cultural method of Bei Shi Olympic mite according to claim 1, is characterized in that: the Bei Shi Olympic mite of turning out, as the forage mites of cultivating carnivorous mite class, is applied to and cultivates carnivorous mite class.
3. the application of the cultural method of Bei Shi Olympic mite according to claim 2, it is characterized in that: the application of the cultural method of described Bei Shi Olympic mite is: first in described culture vessel or carrier, cultivate Bei Shi Olympic mite, when in culture vessel or carrier, when Bei Shi Olympic mite in every 0.1 gram of composts or fertilisers of cultivating reaches more than 1, add again carnivorous mite class, and the quantity of carnivorous mite class in every 0.1 gram of composts or fertilisers of cultivating is reached more than 1; Or in culture vessel or carrier, add Bei Shi Olympic mite and carnivorous mite class, its ratio is 1 carnivorous mite class of 1-100 Bei Shi Olympic mite ︰ simultaneously.
4. the application of the cultural method of Bei Shi Olympic mite according to claim 2, is characterized in that: the concrete species of described carnivorous mite class are: Si Shi genus amblyseius berlese amblyseius( typhlodromips) swirskiiathias-Henriot, California genus amblyseius berlese neoseiulus( amblyseius) californicus(McGregor), pseudo-genus amblyseius berlese neoseiulus (Amblyseius) fallacies(Garman), the former genus amblyseius berlese in river amblyseiuseharaiamitai & Swirski, the nearly blind peaceful mite of lemon amblydromalus( amblyseius) limonicusgarman & McGregor, Anderson genus amblyseius berlese amblyseius andersonichant ,regression genus amblyseius berlese amblyseius( iphiseius) degeneransberlese, a kind of little blind peaceful mite typhlodromips( transeius) montdorensisthe very peaceful mite of Schicha, one euseius gallicuskreiter & Tixier.
5. according to the application of the cultural method of the Bei Shi Olympic mite described in claim 1 or 2 or 3, it is characterized in that, the Bei Shi Olympic mite of turning out, after packing, food as local carnivorous mite class on agriculture, woods crop is directly released in agriculture, woods crop, or does harm to the biological control of mite, insect for agriculture, woods crop.
6. the cultural method of Bei Shi Olympic mite according to claim 1, it is characterized in that, the first step: the culture vessel and the lid thereof that are 1 liter by volume clean up, on lid, there is a ventilation mouth that accounts for lid area 1/3rd, ventilative cloth or paper sealing for ventilation mouth, cloth or paper must reach can breathe freely and can prevent the requirement that mite escapes, and more particularly, general printing paper can be used for the present invention;
Second step: 200 grams of vermiculites are put into above-mentioned culture vessel, and then add the yeast of 30 grams, and stir, yeast is dispersed in vermiculite;
The 3rd step: then add the Bei Shi Olympic mite of 10000 in above-mentioned culture vessel, the lid of covered container subsequently;
The 4th step: more above-mentioned culture vessel is moved in culturing room and cultivated, condition of culture: temperature is 25 ℃, relative air humidity is 90%RH; General cultivation can be used for 15-25 days;
The 5th step: when turning out after Bei Shi Olympic mite, add 10000 carnivorous mite classes in the container of above-mentioned cultivation Bei Shi Olympic mite, continue to cultivate under above-mentioned the same terms to obtain a large amount of carnivorous mite classes for 20 days; Or, also can repeat the above-mentioned first step and second step, the 3rd step changes the Bei Shi Olympic mite that adds 150000 in culture vessel into, and then add the carnivorous mite class of 5000, after the lid of covered container, move into after culturing room, under above-mentioned same culture conditions, cultivate 20 days, also can obtain a large amount of carnivorous mite classes.
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CN105794725A (en) * 2016-03-24 2016-07-27 首伯农(北京)生物技术有限公司 Method for breeding predatory mites and prey mites thereof
CN109699834A (en) * 2017-10-25 2019-05-03 石河子大学 A kind of man-made feeds of the new small peaceful mite of double tails

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CN109699834A (en) * 2017-10-25 2019-05-03 石河子大学 A kind of man-made feeds of the new small peaceful mite of double tails

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