CN103875418B - Method for producing comospore veronica sara-banda - Google Patents
Method for producing comospore veronica sara-banda Download PDFInfo
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- CN103875418B CN103875418B CN201410115664.2A CN201410115664A CN103875418B CN 103875418 B CN103875418 B CN 103875418B CN 201410115664 A CN201410115664 A CN 201410115664A CN 103875418 B CN103875418 B CN 103875418B
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Abstract
The invention relates to a method for producing comospore veronica sara-banda. The method for producing comospore veronica sara-banda includes the steps of planting and processing. The panting method of the comospore veronica sara-banda includes the steps of treating seeds, preparing soil, sowing the seeds, performing management in the seedling stage, performing treatment for passing the winter, transplanting and planting seedlings in the field, performing daily field management and harvesting. In the aspects of safety, effectiveness, stable quality and feature preservation of Tibetan medicine, an artificial planting and production method of Tibetan medicine is researched and developed, and the obtained Tibetan medicine comospore veronica sara-banda produced with the method is stable in quality, safe and effective.
Description
Technical field
The present invention relates to a kind of production method of Herba Veronicae, belong to Chinese medicine production technical field.
Background technology
The Veronica that uses in Tibetan medicine (Dong Dong Na red, draft BAXIAGA), to indicate the Herba Veronicae Veronica eriogyne H.Winkl.Rep.Nov.Beih.12:480.1922 that produces in most area, Tibet for certified products.Tibetan medicine and pharmacology research institute of Tibet Autonomous Region, the understanding of everybody traditional Tibetan medicine expert in land in 2009, acquire sample in kind, and through Plant Inst. doctor, Chinese medicine institute of Shanghai Univ. of Traditional Chinese Medicine dream ten million doctor qualification, be defined as Herba Veronicae Veronica eriogyne H.Winkl.Rep.Nov.Beih..Therefore, the Veronica that Tibet is conventional, namely " Jingzhubencao " " Dong Dong Na red, draft BAXIAGA " of recording, is actually Scrophulariaceae Veronica (Veronica Linn.) plant comospore grandmother.
Herba Veronicae is distributed in China Qinghai-Tibet Platean and the northwestward, with the Partial Species of equal plant lousewort seemingly, belong to High aititude and psychrophyte, be use very general medicinal plant in China's traditional medicine Tibetan medicine, its herb can be used as medicine, and has the effects such as clearing heat and detoxicating, expelling wind and removing dampness, cure mainly hepatitis, cholecystitis, rheumatalgia, nettle rash etc.Up to now, at traditional Tibetan medicine pharmacy corporation, Herba Veronicae raw medicinal material all derives from wild resource, in the whole nation and even the whole world, yet there are no the relate art literature delivering the cultivation of Herba Veronicae artificial planting.Due to this species natural propagation ability extreme difference, add huge raw materials requirement, the wild natural resources of this species is in and Endangered status at present, the intensive population of these species has been can not find in suitable habitats, cause the reduce or stop production of the nearly 50 kinds of tibetan traditional medicine kinds of a lot of traditional Tibetan medicine manufacturing enterprises, not only huge economic loss is caused to enterprise, and have influence on the daily diagnosis and treatment of Liao Ge Tibetan medicine hospital and the normal therapeutic of patient.Therefore, Herba Veronicae is a collection of large Tibetan medicine material of representative, along with the continuous expansion of industry size, along with market is to the increase of Tibetan medicine product demand, generally occur that wild resource cannot meet the situation of Production requirement, therefrom also related go out the safety of industry Arrested Development, conventional tibetan traditional medicine supply difficulty, medication and unstable etc. the series of problems of drug quality.From safe and effective, the steady quality of Tibetan medicine and the angle guaranteeing characteristic, active research explores the artificial planting culture technique of Tibetan medicine material, greatly develops Tibetan medicine material and to standardize artificial production industry, realize industry development and the joint-action mechanism optimizing resource.
Summary of the invention
The present invention on the impact of its seed germination by different condition of culture and pretreatment, is established the method improving seed germination rate, finally provides a kind of production method of Endangered Medicinal Herb.
The present invention adopts following technical scheme to achieve these goals:
The production method of Herba Veronicae, comprises the following steps:
(1) plant
(2) process
The implantation methods of described Herba Veronicae comprises following content:
1, seed treatment
2, wholely
3, sow
4, seedling management
5, to pass the winter process
6, transplant planting
7, daily field management
8, gather
Preferably, wherein seed treatment step, comprises seed soaking and HORMONE TREATMENT, and seed soaking is that water content reaches 40-50% with water soaking seed 1-4 hour before sowing, preferably seed soaking 2 hours, and water content reaches 44.44% and is advisable.
HORMONE TREATMENT
In order to improve seed germination rate and seedling percent under artificial environment, we control HORMONE TREATMENT under 15 DEG C of natural care conditions, use the gibberellin (GA) of 100ppm, seed soaking 1-4 hour, preferred 2h, after rinsed clean, dry in the air in the cool to half-dried, put into glass container, under 0 DEG C of condition, refrigeration 20-30 hour, 24 hours.
Wherein, refer to wholely carry out soil property upgrading before planting, dig, sterilization, rake are thin etc. works wholely; Concrete grammar is, according to the soil on sowing ground, with slant acidity sandy loam for benchmark soil moved in improve the original, and every mu of mixed farmyard manure 300-400kg, preferred 333kg, mixing humus soil 130-170kg, preferred 150kg, climbs carefully wholely, for subsequent use.
Wherein, in sowing step, the sowing time is chosen as before and after annual April 15 beginning of spring in 5 days, adopts mixed-matrix dilution method, determines seed: fine sand=1: the dilution ratio of 10 is broadcasted sowing.
Seedling management, irrigates and adopts dropper method or spraying irrigation method, sooner or later nice and cool period or cloudy day, within every 4 days, irrigate once, and once fill with thoroughly, when need wait until that seedling grows to 4-8cm, carry out weeding work seedling stage.
Pass the winter process, after thinning, Herba Veronicae seedling will not be transplanted immediately, in seedbed, carry out overwintering, the beginning of spring in the coming year carries out transplant planting again, these technical measures, play the survival of the fittest under natural conditions on the one hand, two aspects strengthen the resistance of actual plantation plant.At overwintering period, most important control measures water foot to freeze water, and it is fertile to supplement field table, and fertilizer mainly selects animal fertilizer, by every mu of 1200-1800 jin, preferably uses for 1600 jin.
Transplant planting, during the transplant planting of Herba Veronicae seedling is annual March 23 to April 10 in the beginning of spring, transplant planting selected plant is 15x20 planting density specification apart from x line-spacing.During transplant planting, farmers''s animal fertilizer mainly selected by base fertilizer, and rate of fertilizer is 1.7 tons/mu.
Daily field management, after Herba Veronicae field planting is transplanted, during annual June 10 to June 20, start to enter for a long time prosperous, during annual May 15 to 31, require to topdress once, fertilizer variety mainly selects the organic manure such as animal fertilizer, liquid dung, rate of fertilizer is 0.5 ton/mu, adopts ditch to bury, or mixed water-reducible method adds at plant root.
Within every 5-7 days, irrigate and once select to irrigate in morning and evening or cloudy day, take flooding irrigation method, avoid repeatedly a small amount of during irrigation, and once should fill with foot filling thoroughly.
Gathering of medicinal material, collecting time is selected, annual 15-25 day in June, herb of gathering.
Processing, owing to just locating to grow the vigorous stage, top forms petal simultaneously, be characterized in moisture many, petal place easily goes mouldy infested, therefore, the fresh medicinal material of gathering is sure not to stack, and after should rinsing the earth foreign material be infected with on medicinal material in time, place of selecting to dry in the shade spreads out and dries.Packing packing, packaging should indicate the place of production.
Accompanying drawing explanation
Fig. 1 five crowdes of Veronica medicinal materials fingerprint stacking charts.
Embodiment
, seed treatment
Seed soaking
According to test, the seed storing a year is when being 8.99% without its water content when any process, and this does not reach the needs of normal seed germination completely, therefore, we have carried out the test of seed imbibition, and determine to sow the best seed soaking condition 2h of front seed, and water content can reach 44.44%;
HORMONE TREATMENT
We are by carrying out the activity test of Herba Veronicae inhibition of seed germination thing, there is inhibitory action to a certain degree in the sprouting to a variety of Chinese cabbage and wheat seed of Herba Veronicae seed alcohol extract and water extract, this is also, in natural habitat, the key factor that this species self-reproduction ability is weak, therefore, in order to improve seed germination rate and seedling percent under artificial environment, we have filtered out best HORMONE TREATMENT condition, namely under 15 DEG C of natural care conditions, use the GA of 100ppm, seed soaking 2h, after rinsed clean, dry in the air to half-dried in the cool, put into glass container, under 0 DEG C of condition, refrigeration 24h.
, whole
Herba Veronicae and Scrophulariaceae Veronica Herba Veronicae Lhasa subspecies, belong to High aititude hardy plant, and half cloudy happiness is wet, and slant acidity sandy loam, needs the organic matter of high level.According to this habitat conditions, carry out soil property upgrading before planting, dig, sterilization, rake are thin etc. works wholely.Concrete grammar is, according to the soil on sowing ground, with slant acidity sandy loam for benchmark soil moved in improve the original, and every mu of mixed farmyard manure 333kg, mixing humus soil 150kg, climbs carefully wholely, for subsequent use.
, sowing
Sowing time is chosen as annual about 15 days April of the beginning of spring.Herba Veronicae thousand grain weigth is only 0.04263g, seed is very small, adopt conventional purebred broadcast very difficult, therefore, in sowing link, we adopt mixed-matrix dilution method, we have carried out (seed: humus=1: dilution ratio 3,1: 5,1: 10) respectively, as a result 1: 3 dilution ratio due to seedling overstocked and affect survival rate of seedlings, the dilution ratio even density of 1: 10, survival rate of seedlings is better, and therefore finally determine seed: fine sand=1: the dilution ratio of 10 is broadcasted sowing, lower weight is 0.007-0.01g/m
2.
, seedling management
Seed after planting, emerges after 10 to 12 days in nursery field, within about 20 days, enters the whole bed seeding stage.The newborn seedling of Herba Veronicae is due to reasons such as seed matrix differences, shoot root is very very thin, there is the difficulty that irrigation is inconvenient and weeding difficulty is large in emphasis in management, therefore, by the summary of many years of experience, irrigate seedling stage and adopt dropper method or spraying irrigation method, irrigate practical choice at morning and evening nice and cool period or cloudy day, within every 4 days, irrigate once, and once fill with thoroughly.Young plant weeding aspect, because shoot root is very thin, when need wait until that seedling grows to about 5cm, in conjunction with thinning work, carries out weeding work, simultaneously when making seedbed, for avoiding perplex by weeds seedling stage, accomplishes dusting cover field soil and mixed fertilizer as far as possible.
, process of passing the winter
After thinning, Herba Veronicae seedling will not be transplanted immediately, in seedbed, carry out overwintering, and the beginning of spring in the coming year carries out transplant planting again, these technical measures, plays the survival of the fittest under natural conditions on the one hand, and two aspects strengthen the resistance of actual plantation plant.At overwintering period, most important control measures water foot to freeze water, and it is fertile to supplement field table, and fertilizer mainly selects animal fertilizer, uses by 1600 jin every mu.
, transplant planting
The transplant planting of Herba Veronicae seedling, main selects two time periods to carry out, one be annual March 23 to April 10 in the beginning of spring during, another be annual mid-November after autumn, after Herba Veronicae seedling ground branches and leaves are withered, excavates underground rhizome transplanting.In actual cultivation, two time periods all can select, and have no significant effect, but both also distinguish to some extent to medical material quanlity.The former, during due to transplant planting, existing phenomenon of emerging, certain influence is had to root yield then, but the loss being easy to find individual plant and reducing seedling, the latter is owing to directly transplanting underground rhizome, can better ensure next year root yield, but easily lose seedling in transplanting process.
In transplant planting link, topmost sport technique segment is the assurance of planting density, and we find in actual production process, the excessive easy appearance lodging of density, and density is crossed and dredged easily raw weeds and waste soil.For this reason, we have carried out transplant planting density test specially, we are provided with (spacing in the rows x line-spacing) cm[(15x15) cm, (15x20) cm, (20x20) cm] three grades of planting density specifications, by the investigation record in May to August, find in the Plant Density Experiment field of (15x15) cm, obvious appearance lodging phenomenon, individual plant weight is significantly too small, (20x20) in cm Plant Density Experiment field, Weed infestation is obvious, root yield is affected, (15x20) the Plant Density Experiment field of cm, lodging phenomenon, individual plant weight, overall yield, the inspection target such as Weed infestation are all superior to all the other two specifications, therefore, in actual artificial planting cultivation, the transplant planting phase select (spacing in the rows x line-spacing) cm[(15x20) cm] and kind matter density specification.
During transplant planting, farmers''s animal fertilizer mainly selected by base fertilizer, and rate of fertilizer is 1.7 tons/mu.In addition, after transplant planting completes, there is no special field management requirement, take conventional field crop field control measures.
, daily field management
Herba Veronicae seedling after transplant planting, starts the growth entering the medicinal material phase.The medicinal part of Herba Veronicae is herb or acrial part.In actual production process, in order to protect wild Herba Veronicae resource, more promotion acrial parts are medicinal raw material.
Herba Veronicae artificial planting, enters medicinal material after date, with regard to the growth of plant itself, no longer includes very large obstacle, sturdy particularly along with organs such as rhizomes, in field management, takes conventional field management method just can meet cultivation demand.
After Herba Veronicae field planting is transplanted, during annual June 10 to June 20, start to enter for a long time prosperous, i.e. vegetative growth phase, now higher to the demand of nutriment, therefore, during annual May 15 to 31, require to topdress once, fertilizer variety mainly selects the organic manure such as animal fertilizer, liquid dung, rate of fertilizer is 0.5 ton/mu, adopts ditch to bury, or mixed water-reducible method adds at plant root.
Herba Veronicae after transplant planting, is different from seedling stage, sturdy along with plant roots and stems, and daily irrigation can take flooding irrigation method completely, within every 5 to 7 days, irrigates once, select sooner or later or the cloudy day irrigate, avoid repeatedly a small amount of during irrigation, and once should fill with foot and fill with thoroughly.
In vegetative growth phase, the grown place that Herba Veronicae is received is subject to weeds puzzlement, pass through many years of experience, except artificial weeding is necessary prevention and controls, in order to contain that weeds overgrow, select rational planting density very crucial, by the population Predominant control weed growth of Herba Veronicae, suitable planting density is: (spacing in the rows x line-spacing) cm[(15x20) cm].
, the gathering of medicinal material
Herba Veronicae is used as medicine with herb or acrial part, therefore, collecting time select exist, plant from the vegetative reproduction stage excessively to the transition stage of generative growth phase.In actual production process, transition stage is on annual June about 20.In the selection of collecting time, we have also made special comparative trial in 2012, and by fingerprint map analyzing, acquisition time is too early with excessively late, and when composition all can be caused identical, the situation that contrast component content is significantly low, therefore also affects commodity value.Such as: (being collected in the sample in Linzhi Lu Na town on August 23rd, 2011), liquid-phase chromatograph finger print atlas compares with reference to sample (the medicinal sample of sweet dew Tibetan medicine), and similarity evaluation software (" similarity evaluation 2004 editions " assisted by machine as calculated, Chinese Pharmacopoeia Commission) calculate, similarity is greater than 0.9, conforms with the regulations.Compare with finger-print (common pattern method of formation generates) with contrasting, and similarity evaluation software (" similarity evaluation 2004 editions " assisted by machine as calculated, Chinese Pharmacopoeia Commission) calculate, similarity is 0.899, be less than 0.9, against regulation; But main chemical compositions is substantially identical, just content difference comparatively large (on the low side).
, processing
The medicinal material of gathering, owing to just locating to grow the vigorous stage, top forms petal simultaneously, be characterized in moisture many, petal place easily goes mouldy infested, therefore, the fresh medicinal material of gathering is sure not to stack, and after should rinsing the earth foreign material be infected with on medicinal material in time, place of selecting to dry in the shade spreads out and dries.Medicinal material after drying can directly be packed packing, and packaging should indicate:
Embodiment 2 sprouting condition screens
Herba Veronicae black and amber seed are used 0ppm respectively, after the gibberellin of 100ppm, 200ppm, 300ppm soaks 24h, using husky and filter paper as matrix, cultivates in culture dish.Each culture dish 100 seeds, each process 3 repetition.Sprouting test 24h adds up 1 seed germination number.Cultivation temperature is respectively 4 DEG C, 15 DEG C.Light treatment is respectively full dark, 8h illumination/16h is dark, and intensity of illumination is 6000lx.Nature group cultivation temperature is 28-32 DEG C in addition, and light application time is 13.5h.
Embodiment 3 transplant planting density test
We find in actual production process, the excessive easy appearance lodging of density, and density is crossed and dredged easily raw weeds and waste soil.For this reason, we have carried out transplant planting density test specially, we are provided with (spacing in the rows x line-spacing) cm[(15x15) cm, (15x20) cm, (20x20) cm] three grades of planting density specifications, by the investigation record in May to August, find in the Plant Density Experiment field of (15x15) cm, obvious appearance lodging phenomenon, individual plant weight is significantly too small, (20x20) in cm Plant Density Experiment field, Weed infestation is obvious, root yield is affected, (15x20) the Plant Density Experiment field of cm, lodging phenomenon, individual plant weight, overall yield, the inspection target such as Weed infestation are all superior to all the other two specifications, therefore, in actual artificial planting cultivation, the transplant planting phase select (spacing in the rows x line-spacing) cm[(15x20) cm] and kind matter density specification.In vegetative growth phase, the grown place that Herba Veronicae is received is subject to weeds puzzlement, pass through many years of experience, except artificial weeding is necessary prevention and controls, in order to contain that weeds overgrow, select rational planting density very crucial, by the population Predominant control weed growth of Herba Veronicae, suitable planting density is: (spacing in the rows x line-spacing) cm[(15x20) cm].
Embodiment 4 collecting time is selected
In the selection of collecting time, we have also made special comparative trial in 2012, and by fingerprint map analyzing, acquisition time is too early with excessively late, and when composition all can be caused identical, the situation that contrast component content is significantly low, therefore also affects commodity value.Such as: (being collected in the sample in Linzhi Lu Na town on August 23rd, 2011), liquid-phase chromatograph finger print atlas compares with reference to sample (the medicinal sample of sweet dew Tibetan medicine), and similarity evaluation software (" similarity evaluation 2004 editions " assisted by machine as calculated, Chinese Pharmacopoeia Commission) calculate, similarity is greater than 0.9, conforms with the regulations.Compare with finger-print (common pattern method of formation generates) with contrasting, and similarity evaluation software (" similarity evaluation 2004 editions " assisted by machine as calculated, Chinese Pharmacopoeia Commission) calculate, similarity is 0.899, be less than 0.9, against regulation; But main chemical compositions is substantially identical, just content difference comparatively large (on the low side).
Embodiment 5 stores each condition germination rate of seed of a year
Cultivate each group of seed for 4 DEG C all not germinate.
Cultivate under each condition of black seeds and all do not germinate.
Natural lighting group, dark group, the amber seeds that 15 DEG C of light groups, dark group are cultivated are sprouted curves and are seen the following form respectively:
The each condition germination rate of amber seed storing 1 year sees the following form:
The each condition germination rate of embodiment 6 new seed
Amber seed then 4 DEG C is cultivated each group and is not all germinateed.15 DEG C of light groups, dark group, the sprouting situations that 25 DEG C of light groups, dark groups are cultivated:
The each condition germination rate of amber seed then sees the following form:
The different buried depth seed germination rate of embodiment 7
In conjunction with sprouting test result, from economic, angle consideration easily, select the GA3 process seed adopting 100ppm, carry out the soil incubation of the red seed of Dong Nadong under the sprouting condition of natural lighting, investigate three different buried depths to the impact of seed germination.The results are shown in following table:
The research of embodiment 8, Veronica finger-print
1, medicinal material test sample is collected
2, chromatographic condition
High performance liquid chromatograph Agilent1200LC
Detector G1315D diode array detector determined wavelength 203nm
Chromatographic column Gemini C18 110A, 4.60 × 250mm, 5 μ column temperatures 30 DEG C
Mobile phase acetonitrile-water flow velocity 1ml/min
3, the preparation of test sample and object of reference solution
The selection of object of reference: according to documents and materials, the chemical composition contained in brooklime mainly contains multiple benzyl carbinol glycoside as acteoside, is anti-inflammatory active ingredient; Iridoid glycosides as Rhizoma Picrorhizae glucoside II, catalposide, there is the aucubin of hepatoprotective effect; The compound such as flavonoid glycoside and organic acid.Binding experiment result, we have selected that chromatographic behavior is more stable and the aucubin that peak area is relatively high and picroside-II are object of reference.
The preparation of object of reference solution: precision takes aucubin respectively, picroside-II reference substance is appropriate, adds methyl alcohol and makes the solution of every 1ml containing 1.6mg, to obtain final product.
Prepared by need testing solution: get this product powder (crossing No. three sieves) about 1g, accurately weighed, puts in 100ml tool plug conical flask, precision adds 50% ethanol 25ml, weighed weight, ultrasonic process (power 250W, frequency 40kHz) 40 minutes, let cool, supply the weight of less loss with 50% ethanol, shake up, 0.45um filter membrane filters, get subsequent filtrate, to obtain final product.
Determination method is accurate respectively draws reference substance solution 1 μ l and need testing solution 10 μ l, injection liquid chromatography, measures, to obtain final product.
, methodological study
Instrument and reagent
Agilent1200 type high performance liquid chromatograph system, is made up of (Agilent company of the U.S.) G1311A quaternary gradient pump, G1315D type diode array detector, the full-automatic injector of G1329A type, ChemStation high performance liquid chromatography chem workstation.XS205DU electronic balance (Mettler Toledo Inc. of Switzerland); Shimadzu UV-2401PC ultraviolet specrophotometer.
Acetonitrile is chromatographically pure (Fisher company), and water is ultra-pure water, and all the other reagent is pure for analyzing.
Standard items:
Aucubin: assay use, 111761-200601, Nat'l Pharmaceutical & Biological Products Control Institute
Picroside-II: assay use, 111596-200402, Nat'l Pharmaceutical & Biological Products Control Institute
Detect sample: 5 batches.3 batch samples pick up from main producing region, or purchased from medicinal material market, main producing region, 2 batches is full artificial cultivation, provides by institute of materia medica of Tibetan hospital.
The each 10 μ l of accurate absorption need testing solution respectively, injection liquid chromatography, measures by above-mentioned chromatographic condition, records the chromatogram in 110 minutes.Obtain five crowdes of Veronica medicinal materials fingerprint stacking charts.
, conclusion
(1) the five batches of medicinal materials collected, are at present through inspection, and liquid-phase chromatograph finger print atlas compares with finger-print with contrasting, and machine assists similarity evaluation software to calculate as calculated, and wherein four batches of similarities are all greater than 0.9, conform with the regulations; But wherein Yaocai3 (being collected in Linzhi Lu Na town) is less than 0.9, against regulation.This batch sample profile color is more withered, is obviously different from other several, has crossed collection season, cause the difference of its profile and chromatographic behavior and content when this batch sample possible gathers.Between the finger-print of Different sources Veronica, there is some difference, reflects the lability of quality of medicinal material, be mainly manifested in chemical composition substantially identical, but content exists difference.
Claims (2)
1. an implantation methods for Herba Veronicae, comprises the following steps:
1) seed treatment
Seed treatment comprises seed soaking and HORMONE TREATMENT,
After rinsed clean, dry in the air in the cool to half-dried, put into glass container, under 0 DEG C of condition, refrigerate 24 hours;
Seed soaking is that water content reaches 40-50% with water soaking seed 1-4 hour before sowing,
HORMONE TREATMENT, under 15 DEG C of natural care conditions, uses the gibberellin of 100ppm, soaks seed 2 hours;
2) wholely
In whole ground step, the soil on sowing ground with slant acidity sandy loam for benchmark soil moved in improve the original, and every mu of mixed farmyard manure 333 ㎏, mixing humus soil 150 ㎏, rake is carefully wholely, for subsequent use;
3) sow
Sowing time is chosen as before and after annual April 15 beginning of spring in 5 days, adopts mixed-matrix dilution method, seed: the dilution ratio of fine sand=1:10 is broadcasted sowing;
4) seedling management
In seedling management, irrigate seedling stage and adopt dropper method or spraying irrigation method, sooner or later nice and cool period or cloudy day, within every 4 days, irrigate once, and once fill with thoroughly, when seedling grows to 4-8cm, carry out weeding thinning work;
5) to pass the winter process
After thinning, Herba Veronicae carries out overwintering in seedbed, overwintering period, waters foot and freezes water, and it is fertile to supplement field table, and fertilizer mainly selects animal fertilizer, and use by 1600 jin every mu, the beginning of spring in the coming year carries out transplant planting again;
6) transplant planting
Transplant planting is during March 23 to April 10 in the beginning of spring, and spacing in the rows * line-spacing is the planting density of 15*20cm, and farmyard manure selected by base fertilizer, and rate of fertilizer is 1.7 tons/mu;
7) daily field management
After transplant planting, during annual May 15 to 31, requirement is topdressed once, fertilizer variety mainly selects the organic manure such as animal fertilizer, liquid dung, and rate of fertilizer is 0.5 ton/mu, adopts ditch to bury, or mixed water-reducible method adds at plant root, daily irrigation takes flooding irrigation method completely, within every 5 to 7 days, irrigates once, once should fill with foot and fill with thoroughly;
8) gather
Collecting time was selected in 15-25 day in June, herb of gathering, after should rinsing the earth foreign material be infected with on medicinal material in time, dry;
2. the production method of Herba Veronicae according to claim 1, herb gather after drying place of selecting to dry in the shade spread out and dry.
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