CN103875418A - Method for producing comospore veronica sara-banda - Google Patents
Method for producing comospore veronica sara-banda Download PDFInfo
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Abstract
The invention relates to a method for producing comospore veronica sara-banda. The method for producing comospore veronica sara-banda includes the steps of planting and processing. The panting method of the comospore veronica sara-banda includes the steps of treating seeds, preparing soil, sowing the seeds, performing management in the seedling stage, performing treatment for passing the winter, transplanting and planting seedlings in the field, performing daily field management and harvesting. In the aspects of safety, effectiveness, stable quality and feature preservation of Tibetan medicine, an artificial planting and production method of Tibetan medicine is researched and developed, and the obtained Tibetan medicine comospore veronica sara-banda produced with the method is stable in quality, safe and effective.
Description
Technical field
The present invention relates to a kind of production method of Herba Veronicae, belong to Chinese medicine production technical field.
Background technology
The Veronica that uses in Tibetan medicine (Dong Dong Na is red, draft BAXIAGA), to indicate the Herba Veronicae Veronica eriogyne H.Winkl.Rep.Nov.Beih.12:480.1922 producing in most area, Tibet as certified products.Tibetan medicine and pharmacology research institute of Tibet Autonomous Region, in land in 2009, everybody traditional Tibetan medicine is learned expert's understanding, gather sample in kind, and through the doctor of Plant Inst., Chinese medicine institute of Shanghai Univ. of Traditional Chinese Medicine dream ten million doctor's qualification, be defined as Herba Veronicae Veronica eriogyne H.Winkl.Rep.Nov.Beih..Therefore, the Veronica that Tibet is conventional, " Dong Dong Na is red, draft BAXIAGA " that " Jingzhubencao " recorded, is actually Scrophulariaceae Veronica (Veronica Linn.) plant comospore grandmother.
Herba Veronicae is distributed in China Qinghai-Tibet Platean and the northwestward, with the Partial Species of equal plant lousewort seemingly, belong to high height above sea level and psychrophyte, be in China's traditional medicine Tibetan medicine, to use very general medicinal plant, its herb can be used as medicine, and has the effects such as clearing heat and detoxicating, expelling wind and removing dampness, cure mainly hepatitis, cholecystitis, rheumatalgia, nettle rash etc.Up to now, at traditional Tibetan medicine pharmacy corporation, Herba Veronicae raw medicinal material all derives from wild resource, in the whole nation and even the whole world, yet there are no the correlation technique document of delivering the cultivation of Herba Veronicae artificial planting.Due to this species natural propagation ability extreme difference, add huge raw materials requirement, at present the wild natural resources of this species plant in and Endangered status, in suitable habitats, can not find the intensive population of these species, cause the reduce or stop production of the nearly 50 kinds of tibetan traditional medicine kinds of a lot of traditional Tibetan medicine manufacturing enterprise, not only enterprise is caused to huge economic loss, and had influence on the daily diagnosis and treatment of Liao Ge Tibetan medicine hospital and patient's normal therapeutic.Therefore, Herba Veronicae is a collection of large Tibetan medicine material of representative, along with the continuous expansion of industry size, along with the increase of market to Tibetan medicine product demand, generally occur that wild resource cannot meet the situation of Production requirement, therefrom also related go out industry Arrested Development, conventional tibetan traditional medicine supply with the safety of difficulty, medication and unstable etc. the series of problems of drug quality.From safe and effective, the steady quality of Tibetan medicine with guarantee the angle of characteristic, active research is explored the artificial planting culture technique of Tibetan medicine material, greatly develops the Tibetan medicine material artificial production industry of standardizing, and realizes industry development and the joint-action mechanism of optimizing resource.
Summary of the invention
The present invention's impact on its seed germination by different condition of culture and pretreatment, establishes the method that improves seed germination rate, and a kind of production method of Endangered Medicinal Herb is finally provided.
The present invention adopts following technical scheme to achieve these goals:
The production method of Herba Veronicae, comprises the following steps:
(1) plantation
(2) processing
The implantation methods of described Herba Veronicae comprises following content:
1, seed treatment
2, wholely
3, sowing
4, seedling management
5, the processing of passing the winter
6, transplant planting
7, daily field management
8, gather
Preferably, wherein seed treatment step, comprise seed soaking and HORMONE TREATMENT, seed soaking be sowing before with water soaking seed 1-4 hour, water content reaches 40-50%, preferably soaks seed 2 hours, water content reaches 44.44% and is advisable.
HORMONE TREATMENT
In order to improve seed germination rate and seedling percent under artificial environment, we control HORMONE TREATMENT under 15 DEG C of natural care conditions, use the gibberellin (GA) of 100ppm, seed soaking 1-4 hour, preferably 2h, after rinsing is clean, dry in the air in the cool to half-dried, put into glass container, under 0 DEG C of condition, refrigeration 20-30 hour, 24 hours.
Wherein, referred to before sowing wholely carry out soil property upgrading, dig, sterilization, rake are thin etc. works wholely; Concrete grammar is, according to the soil on sowing ground, and taking slant acidity sandy loam as benchmark soil moved in to improve the original, and every mu of mixed farmyard manure 300-400kg, preferably 333kg, mixes humus soil 130-170kg, and preferably 150kg climbs thin whole ground, for subsequent use.
Wherein, in sowing step, the sowing time was chosen as before and after annual April 15 beginning of spring in 5 days, adopted mixed-matrix dilution method, determined seed: fine sand=1: 10 dilution ratio is broadcasted sowing.
Seedling management, irrigates and adopt dropper method or spraying irrigation method seedling stage, sooner or later nice and cool period or cloudy day, and within every 4 days, irrigate once, and once fill with thoroughly, need wait until when seedling grows to 4-8cm, carry out weeding work.
The processing of passing the winter, after thinning, Herba Veronicae seedling will not be transplanted immediately, in seedbed, carry out overwintering, carry out again transplant planting, these technical measures the beginning of spring in the coming year, play on the one hand the survival of the fittest under natural conditions, two aspects strengthen the resistance of actual plantation plant.At overwintering period, most important control measures are to water foot to freeze water, and supplementary field table is fertile, and fertilizer is mainly selected animal fertilizer, by every mu of 1200-1800 jin, preferably uses for 1600 jin.
Transplant planting, during the transplant planting of Herba Veronicae seedling is annual March 23 to April 10 in the beginning of spring, transplant planting selected plant is 15x20 planting density specification apart from x line-spacing.During transplant planting, base fertilizer is mainly selected farmers''s animal fertilizer, and rate of fertilizer is 1.7 tons/mu.
Daily field management, after Herba Veronicae field planting is transplanted, during annual June 10 to June 20, start to enter prosperous long-term, during annual May 15 to 31, require to topdress once, fertilizer variety mainly selects the organic manure such as animal fertilizer, liquid dung, rate of fertilizer is 0.5 ton/mu, adopts ditch to bury, or mixed water-reducible method is appended at plant root.
Irrigation in every 5-7 days is once chosen in sooner or later or the cloudy day irrigates, and takes flooding irrigation method, avoids repeatedly a small amount of when irrigation, fills with thoroughly and should once fill with foot.
Gathering of medicinal material, collecting time is chosen in, annual 15-25 day in June, the herb of gathering.
Processing, owing to just locating the vigorous stage of growing, top has formed petal simultaneously, be characterized in moisture many, petal place easily goes mouldy infested, therefore, the fresh medicinal material of gathering is sure not to stack, and should rinse in time after the earth foreign material of being infected with on medicinal material, and spread out and dries at the place of selecting to dry in the shade.Packing packing, should indicate the place of production in packaging.
Brief description of the drawings
Five crowdes of Veronica medicinal materials fingerprint stacking charts of Fig. 1.
Embodiment
, seed treatment
Seed soaking
According to test, seed its water content without any processing in the situation that of storing a year is 8.99%, and this does not reach the needs of normal seed germination completely, therefore, we have carried out the test of seed imbibition, and determine the best seed soaking condition of the front seed of sowing 2h, and water content can reach 44.44%;
HORMONE TREATMENT
We are by carrying out the activity test of Herba Veronicae inhibition of seed germination thing, there is inhibitory action to a certain degree to the sprouting of a variety of Chinese cabbage and wheat seed in Herba Veronicae seed alcohol extract and water extract, this is also, in natural habitat, the key factor that this species self-reproduction ability is weak, therefore, in order to improve seed germination rate and seedling percent under artificial environment, we have filtered out best HORMONE TREATMENT condition, under 15 DEG C of natural care conditions, use the GA of 100ppm, seed soaking 2h, after rinsing is clean, dry in the air in the cool to half-dried, put into glass container, under 0 DEG C of condition, refrigeration 24h.
, whole ground
Herba Veronicae is Scrophulariaceae Veronica Herba Veronicae Lhasa subspecies, belongs to high height above sea level hardy plant, and half cloudy happiness is wet, and slant acidity sandy loam needs the organic matter of high level.According to this habitat conditions, carried out soil property upgrading, dig before sowing, sterilization, rake are thin etc. worked wholely.Concrete grammar is, according to the soil on sowing ground, and taking slant acidity sandy loam as benchmark soil moved in to improve the original, and every mu of mixed farmyard manure 333kg, mix humus soil 150kg, climb thin whole ground, for subsequent use.
, sowing
Sowing time was chosen as about annual April 15 beginning of spring.Herba Veronicae thousand grain weigth is only 0.04263g, seed is very small, adopt conventional purebred broadcast very difficult, therefore, in sowing link, we adopt mixed-matrix dilution method, we have carried out respectively the dilution ratio of (seed: humus=1: 3,1: 5,1: 10), the dilution ratio of 1: 3 as a result affects survival rate of seedlings because seedling is overstocked, the dilution ratio even density of 1: 10, survival rate of seedlings is better, therefore finally determines seed: fine sand=1: 10 dilution ratio is broadcasted sowing, lower weight is 0.007-0.01g/m
2.
, seedling management
Seed after planting, emerges after 10 to 12 days in the field of growing seedlings, and enters the whole bed seeding stage about 20 days.The newborn seedling of Herba Veronicae is due to reasons such as seed matrix are poor, shoot root is very very thin, in management, emphasis exists and irrigates inconvenience and the large difficulty of weeding difficulty, therefore, by the summary of many years of experience, irrigate seedling stage and adopt dropper method or spraying irrigation method, irrigate practice and be chosen in sooner or later nice and cool period or cloudy day, within every 4 days, irrigate once, and once fill with thoroughly.Weeding in seedling stage aspect, because shoot root is very thin, need wait until when seedling grows to 5cm left and right, in conjunction with thinning work, carries out weeding work, in the time making seedbed, for avoiding perplexed by weeds, accomplishes dusting cover field soil and mixed fertilizer simultaneously as far as possible.
, the processing of passing the winter
After thinning, Herba Veronicae seedling will not be transplanted immediately, carries out overwintering in seedbed, carries out transplant planting the beginning of spring in the coming year again, and these technical measures play the survival of the fittest under natural conditions on the one hand, and two aspects strengthen the resistance of actual plantation plant.At overwintering period, most important control measures are to water foot to freeze water, and supplementary field table is fertile, and fertilizer is mainly selected animal fertilizer, uses by 1600 jin every mu.
, transplant planting
The transplant planting of Herba Veronicae seedling, mainly selects two time periods to carry out, one be annual March 23 to April 10 in the beginning of spring during, another is mid-November after autumn every year, until Herba Veronicae seedling on the ground branches and leaves withered after, excavate underground rhizome and transplant.In actual cultivation process, two time periods all can select, medical material quanlity is had no significant effect, but both also differences to some extent.The former, during due to transplant planting, the existing phenomenon of emerging, root yield is then had to certain influence, but be easy to find individual plant and the loss that reduces seedling, the latter is owing to directly transplanting underground rhizome, can better ensure next year root yield, but easily lose seedling in transplanting process.
In transplant planting link, topmost sport technique segment is the assurance of planting density, and we find in actual production process, the excessive easy appearance lodging of density, and density is crossed and is dredged easily raw weeds and waste soil.For this reason, we have carried out transplant planting density test specially, we are provided with (spacing in the rows x line-spacing) cm[(15x15) cm, (15x20) cm, (20x20) cm] three grades of planting density specifications, by the investigation record in May to August, find in the Plant Density Experiment field of (15x15) cm, obviously there is lodging phenomenon, individual plant weight is significantly too small, (20x20) in cm Plant Density Experiment field, weeds harm obviously, root yield is affected, (15x20) the Plant Density Experiment field of cm, lodging phenomenon, individual plant weight, overall yield, the investigation indexs such as weeds harm are all superior to all the other two specifications, therefore, in actual artificial planting cultivation process, the transplant planting phase select (spacing in the rows x line-spacing) cm[(15x20) cm] and germplasm density specification.
During transplant planting, base fertilizer is mainly selected farmers''s animal fertilizer, and rate of fertilizer is 1.7 tons/mu.In addition, after transplant planting completes, there is no special field management requirement, take conventional field crop field control measures.
, daily field management
Herba Veronicae seedling after transplant planting, the growth that starts to enter the medicinal material phase.The medicinal part of Herba Veronicae is herb or acrial part.In actual production process, in order to protect wild Herba Veronicae resource, more promotion acrial parts are medicinal raw material.
Herba Veronicae artificial planting, enters medicinal material after date, with regard to the growth of plant itself, no longer includes very large obstacle, and particularly sturdy along with organs such as rhizomes, in field management, takes conventional field management method just can meet cultivation demand.
After Herba Veronicae field planting is transplanted, during annual June 10 to June 20, start to enter prosperous long-term, i.e. vegetative growth phase, now higher to the demand of nutriment, therefore,, during annual May 15 to 31, require to topdress once, fertilizer variety mainly selects the organic manure such as animal fertilizer, liquid dung, rate of fertilizer is 0.5 ton/mu, adopts ditch to bury, or mixed water-reducible method is appended at plant root.
Herba Veronicae after transplant planting, is different from seedling stage, sturdy along with plant roots and stems, and daily irrigation can be taked flooding irrigation method completely, within every 5 to 7 days, irrigates once, is chosen in sooner or later or the cloudy day irrigates, and avoids repeatedly a small amount of when irrigation, fills with thoroughly and should once fill with foot.
In vegetative growth phase, the grown place that Herba Veronicae is received is subject to weeds puzzlement, pass through many years of experience, except artificial weeding is necessary preventing and treating method, in order to contain weeds overgrowing, select rational planting density very crucial, by the population advantage control weed growth of Herba Veronicae, suitable planting density is: (spacing in the rows x line-spacing) cm[(15x20) cm].
, the gathering of medicinal material
Herba Veronicae is used as medicine with herb or acrial part, and therefore, collecting time is chosen in, plant from the vegetative reproduction stage excessively to the transition stage of generative growth phase.In actual production process, transition stage is about annual June 20.In the selection of collecting time, we have also made special comparative trial in 2012, and by fingerprint map analyzing, acquisition time too early and excessively late, all can cause in situation that composition is identical, and the remarkable low situation of contrast component content, therefore also affects commodity value.For example: (being collected in the sample in Linzhi Lu Na town on August 23rd, 2011), liquid-phase chromatograph finger print atlas with reference to sample (the medicinal sample of sweet dew Tibetan medicine) relatively, and machine is assisted similarity evaluation software (" similarity evaluation 2004 editions " as calculated, Chinese Pharmacopoeia Commission) calculate, similarity is greater than 0.9, conforms with the regulations.With contrast with finger-print (generations of common pattern method of formation) comparison, and machine is assisted similarity evaluation software (" similarity evaluation 2004 editions " as calculated, Chinese Pharmacopoeia Commission) calculate, similarity is 0.899, be less than 0.9, against regulation; But main chemical compositions is basic identical, just content differs large (on the low side).
, processing
The medicinal material of gathering, owing to just locating the vigorous stage of growing, top has formed petal simultaneously, be characterized in moisture many, petal place easily goes mouldy infested, therefore, the fresh medicinal material of gathering is sure not to stack, and should rinse in time after the earth foreign material of being infected with on medicinal material, and spread out and dries at the place of selecting to dry in the shade.The packing of can directly packing of medicinal material after drying, should indicate in packaging:
Embodiment 2 sprouting condition screenings
Herba Veronicae black and amber seed are used respectively to 0ppm, 100ppm, 200ppm, the gibberellin of 300ppm soaks after 24h, using husky and filter paper as matrix, in culture dish, cultivates.100 seeds of each culture dish, 3 repetitions of each processing.Sprouting test 24h adds up 1 seed germination number.Cultivation temperature is respectively 4 DEG C, 15 DEG C.Light treatment is respectively full dark, 8h illumination/16h dark, and intensity of illumination is 6000lx.Nature group cultivation temperature is 28-32 DEG C in addition, and light application time is 13.5h.
Embodiment 3 transplant planting density tests
We find in actual production process, the excessive easy appearance lodging of density, and density is crossed and is dredged easily raw weeds and waste soil.For this reason, we have carried out transplant planting density test specially, we are provided with (spacing in the rows x line-spacing) cm[(15x15) cm, (15x20) cm, (20x20) cm] three grades of planting density specifications, by the investigation record in May to August, find in the Plant Density Experiment field of (15x15) cm, obviously there is lodging phenomenon, individual plant weight is significantly too small, (20x20) in cm Plant Density Experiment field, weeds harm obviously, root yield is affected, (15x20) the Plant Density Experiment field of cm, lodging phenomenon, individual plant weight, overall yield, the investigation indexs such as weeds harm are all superior to all the other two specifications, therefore, in actual artificial planting cultivation process, the transplant planting phase select (spacing in the rows x line-spacing) cm[(15x20) cm] and germplasm density specification.In vegetative growth phase, the grown place that Herba Veronicae is received is subject to weeds puzzlement, pass through many years of experience, except artificial weeding is necessary preventing and treating method, in order to contain weeds overgrowing, select rational planting density very crucial, by the population advantage control weed growth of Herba Veronicae, suitable planting density is: (spacing in the rows x line-spacing) cm[(15x20) cm].
Embodiment 4 collecting times are selected
In the selection of collecting time, we have also made special comparative trial in 2012, and by fingerprint map analyzing, acquisition time too early and excessively late, all can cause in situation that composition is identical, and the remarkable low situation of contrast component content, therefore also affects commodity value.For example: (being collected in the sample in Linzhi Lu Na town on August 23rd, 2011), liquid-phase chromatograph finger print atlas with reference to sample (the medicinal sample of sweet dew Tibetan medicine) relatively, and machine is assisted similarity evaluation software (" similarity evaluation 2004 editions " as calculated, Chinese Pharmacopoeia Commission) calculate, similarity is greater than 0.9, conforms with the regulations.With contrast with finger-print (generations of common pattern method of formation) comparison, and machine is assisted similarity evaluation software (" similarity evaluation 2004 editions " as calculated, Chinese Pharmacopoeia Commission) calculate, similarity is 0.899, be less than 0.9, against regulation; But main chemical compositions is basic identical, just content differs large (on the low side).
Embodiment 5 stores the each condition germination rate of seed of a year
Cultivating each group of seed for 4 DEG C does not all germinate.
Under the each condition of black seed, cultivate and all do not germinate.
Natural lighting group, dark group, the amber seeds that 15 DEG C of light groups, dark groups are cultivated are sprouted curves and are seen the following form respectively:
The each condition germination rate of amber seed that stores 1 year sees the following form:
The each condition germination rate of embodiment 6 new seed
4 DEG C of amber seeds are then cultivated each group and are not all germinateed.15 DEG C of light groups, dark group, 25 DEG C of sprouting situations that light group, dark group are cultivated:
The each condition germination rate of amber seed then sees the following form:
The different buried depth seed germination rates of embodiment 7
In conjunction with sprouting test result, from economic, angle consideration easily, select to adopt the GA3 of 100ppm to process seed, under the sprouting condition of natural lighting, carry out the soil incubation of the red seed of Dong Nadong, investigate the impact of three different buried depths on seed germination.The results are shown in following table:
The research of embodiment 8, Veronica finger-print
1, medicinal material test sample is collected
2, chromatographic condition
High performance liquid chromatograph Agilent1200LC
Detector G1315D diode array detector detects wavelength 203nm
Chromatographic column Gemini C18 110A, 4.60 × 250mm, 5 30 DEG C of μ column temperatures
Mobile phase acetonitrile-water flow velocity 1ml/min
3, the preparation of test sample and object of reference solution
The selection of object of reference: according to documents and materials, the chemical composition containing in brooklime mainly contains multiple benzyl carbinol glycoside as acteoside, is anti-inflammatory activity composition; Iridoid glycosides as Rhizoma Picrorhizae glucoside II, catalposide, there is the aucubin of hepatoprotective effect; The compound such as flavonoid glycoside and organic acid.In conjunction with experimental result, aucubin and picroside-II that we have selected that chromatographic behavior is more stable and peak area is relatively high are object of reference.
The preparation of object of reference solution: precision takes aucubin respectively, picroside-II reference substance is appropriate, adds methyl alcohol and makes the solution of every 1ml containing 1.6mg, to obtain final product.
Need testing solution preparation: get the about 1g of this product powder (crossing sieve No. three), accurately weighed, put in 100ml tool plug conical flask, precision adds 50% ethanol 25ml, weighed weight, ultrasonic processing (power 250W, frequency 40kHz) 40 minutes, let cool, the weight of supplying less loss with 50% ethanol, shakes up, and 0.45um filter membrane filters, get subsequent filtrate, to obtain final product.
Determination method is accurate reference substance solution 1 μ l and the need testing solution 10 μ l of drawing respectively, and injection liquid chromatography, measures, and to obtain final product.
, methodological study
Instrument and reagent
Agilent1200 type high performance liquid chromatograph system, forms (Agilent company of the U.S.) by G1311A quaternary gradient pump, G1315D type diode array detector, the full-automatic injector of G1329A type, ChemStation high performance liquid chromatography chem workstation.XS205DU electronic balance (Mettler Toledo Inc. of Switzerland); Shimadzu UV-2401PC ultraviolet specrophotometer.
Acetonitrile is chromatographically pure (Fisher company), and water is ultra-pure water, and all the other reagent are pure for analyzing.
Standard items:
Aucubin: assay use, 111761-200601, Nat'l Pharmaceutical & Biological Products Control Institute
Picroside-II: assay use, 111596-200402, Nat'l Pharmaceutical & Biological Products Control Institute
Detect sample: 5 batches.3 batch samples are picked up from main producing region, or purchased from medicinal material market, main producing region, 2 batches is full artificial cultivation, provides by institute of materia medica of Tibetan hospital.
The accurate each 10 μ l of need testing solution that draw respectively, injection liquid chromatography, measures by above-mentioned chromatographic condition, records the chromatogram in 110 minutes.Obtain five crowdes of Veronica medicinal materials fingerprint stacking charts.
, conclusion
(1) five batches of medicinal materials collecting, are at present through inspection, and liquid-phase chromatograph finger print atlas is used finger-print comparison with contrast, and machine is assisted the calculating of similarity evaluation software as calculated, and wherein four batches of similarities are all greater than 0.9, conform with the regulations; But wherein Yaocai3 (being collected in Linzhi Lu Na town) is less than 0.9, against regulation.This batch sample profile color is more withered, is obviously different from other several, when possible this batch sample gathers, has crossed collection season, causes the difference of its profile and chromatographic behavior and content.Between the finger-print of different places of production Veronica, there is some difference, reflected the lability of quality of medicinal material, be mainly manifested in chemical composition basic identical, but content exists difference.
Claims (10)
1. a production method for Herba Veronicae, comprises the following steps:
(1) plantation
(2) processing;
The implantation methods of described Herba Veronicae comprises following content:
1) seed treatment
2) wholely
3) sowing
4) seedling management
5) processing of passing the winter
6) transplant planting
7) daily field management
8) gather.
2. the production method of Herba Veronicae according to claim 1, is characterized in that, seed treatment comprises seed soaking and HORMONE TREATMENT, seed soaking is with water soaking seed 1-4 hour before sowing, water content reaches 40-50%, preferably soaks seed 2 hours, and water content reaches 44.44% and is advisable.
3. the production method of Herba Veronicae according to claim 2, is characterized in that, HORMONE TREATMENT, under 15 DEG C of natural care conditions, is used the gibberellin of 100ppm, seed soaking 1-4 hour, and 2h preferably soaks seed;
After rinsing is clean, dry in the air in the cool to half-dried, put into glass container, under 0 DEG C of condition, refrigeration 20-30 hour, preferably refrigerates 24 hours.
4. the production method of Herba Veronicae according to claim 1, is characterized in that, sows the soil on ground taking slant acidity sandy loam as benchmark soil moved in to improve the original in whole ground step, and every mu of mixed farmyard manure 333 ㎏, mixes humus soil 150 ㎏, climbs thin whole ground, for subsequent use.
5. the production method of Herba Veronicae according to claim 1, is characterized in that, in sowing step, the sowing time was chosen as before and after annual April 15 beginning of spring in 5 days, adopted mixed-matrix dilution method, and the dilution ratio of seed: fine sand=1:10 is broadcasted sowing.
6. the production method of Herba Veronicae according to claim 1, is characterized in that, in seedling management, irrigate seedling stage and adopt dropper method or spraying irrigation method, sooner or later nice and cool period or cloudy day, within every 4 days, irrigate once, and once fill with thoroughly, when seedling grows to 4-8cm, carry out weeding thinning work.
7. the production method of Herba Veronicae according to claim 1, it is characterized in that, after thinning, Herba Veronicae carries out overwintering in seedbed, and overwintering period waters foot and freezes water, and supplementary field table is fertile, fertilizer is mainly selected animal fertilizer, uses by 1600 jin every mu, carries out transplant planting the beginning of spring in the coming year again.
8. the production method of Herba Veronicae according to claim 1, transplant planting during March 23 to April 10 in the beginning of spring, the planting density that spacing in the rows * line-spacing is 15*20cm, base fertilizer is selected farmyard manure, rate of fertilizer is 1.7 tons/mu.
9. the production method of Herba Veronicae according to claim 1, during annual May 15 to 31, requirement is topdressed once, fertilizer variety mainly selects the organic manure such as animal fertilizer, liquid dung, and rate of fertilizer is 0.5 ton/mu, adopts ditch to bury, or mixed water-reducible method is appended at plant root, flooding irrigation method is taked in daily irrigation completely, within every 5 to 7 days, irrigates once, should once fill with foot and fill with thoroughly.
10. the production method of Herba Veronicae according to claim 1, collecting time is chosen in 15-25 day in June, and the herb of gathering should rinse after the earth foreign material of being infected with on medicinal material in time, dry, and spread out and dry at the place of preferably selecting to dry in the shade.
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| CN113812614A (en) * | 2021-03-04 | 2021-12-21 | 孙晓辉 | Uric acid resistant functional food |
| CN114246118A (en) * | 2021-12-22 | 2022-03-29 | 元合玲 | Cultivation technical method of evergreen Persian herb |
| CN115669483A (en) * | 2021-07-22 | 2023-02-03 | 石河子大学 | Method for promoting ferula seeds to germinate and propagate seedlings of ferula |
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| CN102027855A (en) * | 2010-10-25 | 2011-04-27 | 赤峰天奇制药有限责任公司 | Technology for processing coastal glehnia root medicinal materials in planting and producing area and quality standard |
| CN103262748A (en) * | 2013-06-14 | 2013-08-28 | 湖南麓山天然植物制药有限公司 | GAP (Good Agricultural Practices) cultivation method for medicinal gynostemma pentaphylla under mountain land red earth forests |
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| CA2544954A1 (en) * | 2006-04-28 | 2007-10-28 | Jean-Philippe Poiree-Ville | Soilless cultivation system |
| RU2389174C2 (en) * | 2007-07-27 | 2010-05-20 | Хасян Вагизович Шарафутдинов | Seed mixture for moorish lawn growing |
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| CN101578956A (en) * | 2009-06-22 | 2009-11-18 | 贵州信邦中药发展有限公司 | Standardization planting method of uncaria |
| CN101803518A (en) * | 2010-03-25 | 2010-08-18 | 广州陈李济药厂 | Standardized plating method of Kunming begonia traditional Chinese medicinal materials |
| CN101878717A (en) * | 2010-07-08 | 2010-11-10 | 贵阳德昌祥药业有限公司 | Standardized planting method for buerger lespedeza root |
| CN102027855A (en) * | 2010-10-25 | 2011-04-27 | 赤峰天奇制药有限责任公司 | Technology for processing coastal glehnia root medicinal materials in planting and producing area and quality standard |
| CN103262748A (en) * | 2013-06-14 | 2013-08-28 | 湖南麓山天然植物制药有限公司 | GAP (Good Agricultural Practices) cultivation method for medicinal gynostemma pentaphylla under mountain land red earth forests |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113812614A (en) * | 2021-03-04 | 2021-12-21 | 孙晓辉 | Uric acid resistant functional food |
| CN115669483A (en) * | 2021-07-22 | 2023-02-03 | 石河子大学 | Method for promoting ferula seeds to germinate and propagate seedlings of ferula |
| CN114246118A (en) * | 2021-12-22 | 2022-03-29 | 元合玲 | Cultivation technical method of evergreen Persian herb |
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