CN103864943A - Portulace polysaccharide ultrasonic-auxiliary extraction method - Google Patents
Portulace polysaccharide ultrasonic-auxiliary extraction method Download PDFInfo
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- CN103864943A CN103864943A CN201210550635.XA CN201210550635A CN103864943A CN 103864943 A CN103864943 A CN 103864943A CN 201210550635 A CN201210550635 A CN 201210550635A CN 103864943 A CN103864943 A CN 103864943A
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Abstract
The invention discloses a purslane polysaccharide ultrasonic-auxiliary extraction method, and purslane polysaccharide is extracted by the following steps: drying, crushing, screening, degreasing, ethanol reflux extraction, drying in air, ultrasonic extraction, concentration, alcohol precipitation, centrifugation, washing, drying and the like. Ultrasonic ''cavitation phenomenon'' can produce an instant thousands of PA pressure, tiny bubbles in an extraction medium are compressed, burst and broken, and a target extract is acceleratedly dissolved; diffusion of dissolved components can be intensified by ''mechanical vibration'' and ''thermal effect'', and the whole process is completed in a moment, so that the broken speed is increased, the broken time is shortened, the extraction efficiency is greatly improved, the extraction cost is saved, and the product quality is improved.
Description
Technical field
The invention belongs to extraction process technology field, be specifically related to the method for ultrasonic wave auxiliary extraction Polysaccharide from Portulaca oleracea.
Background technology
Purslane is Portulacaceae annual herb plant, and all there is distribution various places at home, is medicine food dual purpose plant.Herb hyoscine, has clearing heat and promoting diuresis, detoxify and promote the subsdence of swelling, anti-inflammatory, quenches the thirst, diuretic properties, seed materials for improving vision.There are some researches show, the ethanol extraction of purslane and decocting liquid have significant restraining effect to dysentery bacterium, and in addition, Herba portulacae extract also all has restraining effect to intestinal bacteria, Corynebacterium diphtheriae, streptococcus aureus.Polysaccharide is the main biologically active substance of purslane, if can extract Polysaccharide from Portulaca oleracea from purslane and apply it to health care pharmaceutical industries, can increase purslane value-added content of product.But the traditional extraction process of Polysaccharide from Portulaca oleracea exists the shortcomings such as yield is low, loss is large, power consumption is higher at present.
Summary of the invention
The above-mentioned defect existing in order to overcome prior art field, the object of the invention is to, and a kind of method of ultrasonic wave auxiliary extraction Polysaccharide from Portulaca oleracea is provided, solve prior art tradition and extract the method for Polysaccharide from Portulaca oleracea, exist yield low, lose greatlyr, higher problem consumes energy.
The method of ultrasonic wave auxiliary extraction Polysaccharide from Portulaca oleracea provided by the invention, comprise the following steps: by adopt new fresh purslane clean, with 50 ℃ of oven dry of loft drier, then pulverize with pulverizer, 40 orders sieve, 5 times of sherwood oil backflow degreasings 2 times, then 5 times of 95% alcohol reflux 2 times, dry filter residue ventilation, after drying, add 45 times of distilled water, in ultrasonic cleaner with power 130W, extract 55 minutes at 75 ℃, extraction finishes rear filtration extracting solution, filtrate is concentrated to proper volume with vacuum rotary evaporator, get filtrate carries out alcohol and analyses at 4 ℃, centrifugal, gained precipitation 95% ethanol, dehydrated alcohol, washing with acetone for several times, vacuum-drying, obtain Polysaccharide from Portulaca oleracea.
The method of ultrasonic wave auxiliary extraction Polysaccharide from Portulaca oleracea provided by the invention, its beneficial effect is, ultrasonic wave has " cavitation phenomenon ", the characteristic such as " mechanical vibration " and " heat effect ", " cavitation phenomenon " can produce several kpa pressures of moment, make to extract the micro-bubble compression in medium, explosion, broken, accelerate the dissolving of target extract, " mechanical vibration " and " heat effect " have further strengthened the diffusion of dissolved element, whole process completed in moment, thereby improve broken speed, shorten the broken time, greatly improve extraction efficiency, save extraction cost, improve the quality of products.
Embodiment
Below in conjunction with an embodiment, the method for ultrasonic wave auxiliary extraction Polysaccharide from Portulaca oleracea provided by the invention is described in detail.
Embodiment
The method of the ultrasonic wave auxiliary extraction Polysaccharide from Portulaca oleracea of the present embodiment, comprise the following steps: by adopt new fresh purslane clean, with 50 ℃ of oven dry of loft drier, then pulverize with pulverizer, 40 orders sieve, 5 times of sherwood oil backflow degreasings 2 times, then 5 times of 95% alcohol reflux 2 times, dry filter residue ventilation, after drying, add 45 times of distilled water, in ultrasonic cleaner with power 130W, extract 55 minutes at 75 ℃, extraction finishes rear filtration extracting solution, filtrate is concentrated to proper volume with vacuum rotary evaporator, get filtrate carries out alcohol and analyses at 4 ℃, centrifugal, gained precipitation 95% ethanol, dehydrated alcohol, washing with acetone for several times, vacuum-drying, obtain Polysaccharide from Portulaca oleracea, yield is 13.28%.
The present invention adopts ultrasonic wave auxiliary law to extract Polysaccharide from Portulaca oleracea, by experiment of single factor and orthogonal experiment, its extraction process is optimized, to determine optimum process condition.Whole process completed in moment, thereby had improved broken speed, had shortened the broken time, had greatly improved extraction efficiency.
Claims (1)
1. the method for a ultrasonic wave auxiliary extraction Polysaccharide from Portulaca oleracea, it is characterized in that: comprise the following steps: by adopt new fresh purslane clean, with 50 ℃ of oven dry of loft drier, then pulverize with pulverizer, 40 orders sieve, 5 times of sherwood oil backflow degreasings 2 times, then 5 times of 95% alcohol reflux 2 times, dry filter residue ventilation, after drying, add 45 times of distilled water, in ultrasonic cleaner with power 130W, extract 55 minutes at 75 ℃, extraction finishes rear filtration extracting solution, filtrate is concentrated to proper volume with vacuum rotary evaporator, get filtrate carries out alcohol and analyses at 4 ℃, centrifugal, gained precipitation 95% ethanol, dehydrated alcohol, washing with acetone for several times, vacuum-drying, obtain Polysaccharide from Portulaca oleracea.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210550635.XA CN103864943A (en) | 2012-12-18 | 2012-12-18 | Portulace polysaccharide ultrasonic-auxiliary extraction method |
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| CN201210550635.XA CN103864943A (en) | 2012-12-18 | 2012-12-18 | Portulace polysaccharide ultrasonic-auxiliary extraction method |
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| CN103864943A true CN103864943A (en) | 2014-06-18 |
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| CN201210550635.XA Pending CN103864943A (en) | 2012-12-18 | 2012-12-18 | Portulace polysaccharide ultrasonic-auxiliary extraction method |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106117381A (en) * | 2016-06-22 | 2016-11-16 | 陈凌 | A kind of method utilizing pectase to extract Polysaccharide from Portulaca oleracea |
| CN106220750A (en) * | 2016-08-30 | 2016-12-14 | 佛山科学技术学院 | The extracting method of Polysaccharide from Portulaca oleracea |
| CN106361593A (en) * | 2016-09-22 | 2017-02-01 | 防城港市港口区思达电子科技有限公司 | Natural anti-senescence component and preparation method thereof |
| CN107216402A (en) * | 2016-12-23 | 2017-09-29 | 翁绍瑀 | A kind of preparation method of Polysaccharide from Portulaca oleracea |
| CN108498410A (en) * | 2018-06-26 | 2018-09-07 | 康瑞芳 | A kind of anti-dandruff nourishing shampoo of plant essence and preparation method thereof |
| CN108542926A (en) * | 2018-05-23 | 2018-09-18 | 广东工业大学 | The preparation method and pharmaceutical composition of a kind of purslane extract and its application |
| CN110477191A (en) * | 2019-09-02 | 2019-11-22 | 广州纳多利生物科技有限公司 | A kind of preparation method of son's pig feed additive |
| CN110483655A (en) * | 2019-08-30 | 2019-11-22 | 山东花物堂生物科技有限公司 | A kind of Polysaccharide from Portulaca oleracea extraction process and its application in skin care item |
-
2012
- 2012-12-18 CN CN201210550635.XA patent/CN103864943A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106117381A (en) * | 2016-06-22 | 2016-11-16 | 陈凌 | A kind of method utilizing pectase to extract Polysaccharide from Portulaca oleracea |
| CN106220750A (en) * | 2016-08-30 | 2016-12-14 | 佛山科学技术学院 | The extracting method of Polysaccharide from Portulaca oleracea |
| CN106361593A (en) * | 2016-09-22 | 2017-02-01 | 防城港市港口区思达电子科技有限公司 | Natural anti-senescence component and preparation method thereof |
| CN107216402A (en) * | 2016-12-23 | 2017-09-29 | 翁绍瑀 | A kind of preparation method of Polysaccharide from Portulaca oleracea |
| CN108542926A (en) * | 2018-05-23 | 2018-09-18 | 广东工业大学 | The preparation method and pharmaceutical composition of a kind of purslane extract and its application |
| CN108498410A (en) * | 2018-06-26 | 2018-09-07 | 康瑞芳 | A kind of anti-dandruff nourishing shampoo of plant essence and preparation method thereof |
| CN110483655A (en) * | 2019-08-30 | 2019-11-22 | 山东花物堂生物科技有限公司 | A kind of Polysaccharide from Portulaca oleracea extraction process and its application in skin care item |
| CN110477191A (en) * | 2019-09-02 | 2019-11-22 | 广州纳多利生物科技有限公司 | A kind of preparation method of son's pig feed additive |
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Application publication date: 20140618 |