CN101323649B - Method for extracting mushroom polysaccharide by using ultra-high pressure - Google Patents
Method for extracting mushroom polysaccharide by using ultra-high pressure Download PDFInfo
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- CN101323649B CN101323649B CN2008101505332A CN200810150533A CN101323649B CN 101323649 B CN101323649 B CN 101323649B CN 2008101505332 A CN2008101505332 A CN 2008101505332A CN 200810150533 A CN200810150533 A CN 200810150533A CN 101323649 B CN101323649 B CN 101323649B
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Abstract
The invention discloses a method for extracting shiitake polysaccharides from shiitakes, which overcomes the defects that the existing extracting method requires long time, has low efficiency and high energy consumption and damages active ingredients of the shiitake polysaccharides. The proposal adopted by the invention comprises the following steps of: crushing the selected dry shiitakes, adding extract, processing under super-high pressure, filtering, concentrating in vacuum, precipitating with alcohol, drying and finally obtaining shiitake polysaccharides. The high pressure is adopted by the invention to hold or relieve pressure of the shiitake extract, increase the differences between osmotic pressures in and out of cells and change the structure of cells, thereby easily dissolving out effective ingredients, keeping the physiologically active ingredients unchanged, killing a part of microorganisms by high-pressure processing and finishing extraction and sterilization in one step. Being a cold extracting technique, the super-high-pressure extracting method generates no high temperature during the whole extraction process, shortens the extracting time to a few minutes, conserves the effective ingredients, and has significant economic benefits and broad prospect of market development.
Description
Technical field
The present invention relates to a kind of method of from mushroom, extracting lentinan, specifically is a kind of method of utilizing ultra-high voltage to extract lentinan, belongs to field of medicinal materials processing.
Background technology
China is Edible Fungi big country, and wherein the output of mushroom occupies first place in the world.Mushroom contains rich in protein; Nutritive ingredients such as amino acid, fat, glucide, multivitamin and mineral substance, wherein the lentinan in the glucide have antiviral, antitumor, regulate immunologic function and stimulate multinomial functions such as Interferon, rabbit formation.Lentinan is not only a kind of more satisfactory medicine, also is one type of important protective foods functional factor, is current medicine and foodstuffs industry Focal Point of Common Attention, has the wide development application prospect.
At present, the most frequently used process for extracting of lentinan is a water extraction method, and this method extraction time is long, efficient is low, energy consumption is big, has also destroyed the activeconstituents of lentinan simultaneously.In recent years; Also the someone utilizes enzyme process, microwave method or ultrasonic extraction method lentinan, though these methods have improved polysaccharide yield to a certain extent, extraction time still was thirty minutes long 60 minutes; Cost is high, causes sex change inactivation, the radiation effect HUMAN HEALTH of effective constituent easily.Therefore, the process for extracting of a kind of lentinan fast and effectively of exploitation has become the key of lentinan product development.Utilize the ultra-high voltage extractive technique to extract lentinan, at home and abroad do not appear in the newspapers as yet at present.
Summary of the invention
The objective of the invention is to disclose a kind of method of utilizing ultra-high voltage rapid extraction lentinan, overcome the defective that present multiple extraction lentinan method exists, constant with the activeconstituents that guarantees extraction back lentinan.
The present invention accomplishes through the following steps preparation:
A. select dry mushroom;
B. pulverize;
C. add extracting solution;
D. uhp treatment;
E. filter;
F. vacuum concentration;
G. ethanol sedimentation;
H. dry;
I. lentinan.
The present invention adopts high pressure that the mushroom feed liquid is carried out pressurize, and release rapidly then increases the inside and outside permeable pressure head of cell; The structure of cell is changed, thereby make its effective constituent be able to rapid stripping, ultrahigh-pressure extraction method is a kind of cold extraction technology; In whole leaching process, can not produce high temperature, so kept the original physiologically active ingredient of mushroom constant, autoclaving has been killed the part mikrobe simultaneously; Make to extract with one step of sterilization and accomplish, extraction time can foreshorten to several minutes.
Embodiment
Concrete operations step of the present invention is following:
A. select dry mushroom
B. pulverize
C. add pure water
C.1 the mushroom after will pulverizing adds in the pure water, and its quality proportioning is 1: 1-50;
D. uhp treatment
D.1 pressure 100～600MPa
D.2 temperature is 20 ℃～80 ℃
D.3 dwell time 1～10min
D.4 release 1～3 time repeats to boost;
E. filter
F. vacuum concentration
F.1 temperature is 40 ℃～70 ℃
F.2 vacuum tightness 85～98KPa
F.3 time 30～90min;
G. ethanol sedimentation
G.1 95% ethanol that adds 2～5 times of liquid concentrator volumes
G.2 pure shallow lake time 4～15h;
H. dry
H.140 ℃～70 ℃ of oven dry;
I. lentinan.
Provide following examples, but the present invention is not limited to present embodiment at present:
A. select dry mushroom
B. pulverize
B.1 pulverized 20 orders;
C. add pure water
C.1 the mushroom after will pulverizing adds in the pure water, and its quality proportioning is 1: 30;
D. uhp treatment
D.1 pressure 400MPa
D.2 temperature is 50 ℃
D.3 dwell time 6min
D.4 release 2 times repeat to boost;
E. filter
F. vacuum concentration
F.1 thickening temperature is 50 ℃
F.2 vacuum tightness 85KPa
F.3 concentration time 45min;
G. ethanol sedimentation
G.1 95% ethanol that adds 3 times of liquid concentrator volumes
G.2 pure shallow lake time 8h;
H. dry
H.150 ℃ oven dry;
I. lentinan.
The present invention has thoroughly solved the defective that present lentinan process for extracting exists, and its technology is simple, polysaccharide yield is high, foreign matter content is few, cost is low.Can more effectively preserve the activeconstituents of lentinan, strengthen effect.A complete set of technology is simple, easy to operate, continuity is good, is prone to realize industriallization, products obtained therefrom steady quality, safety.Solved the technological barrier that present lentinan extraction efficiency is low, cost is high, foreign matter content is high.Change the rare present situation of high-quality in the market lentinan, realized the increment of lentinan.The present invention simultaneously also provides a kind of novel process fast and efficiently for the extraction of pharmaceutically active ingredient in other, has the huge economic benefit and the prospect of marketing.
Claims (2)
1. method of utilizing ultra-high voltage to extract lentinan is characterized in that being undertaken by following concrete operations step:
A. select dry mushroom;
B. pulverize;
C. add pure water,
C.1 the mushroom after will pulverizing adds in the pure water, and its quality proportioning is 1: 1-50;
D. uhp treatment,
D.1 pressure 100～600MPa,
D.2 temperature is 20 ℃～80 ℃,
D.3 dwell time 1～10min,
D.4 release 1～3 time repeats to boost;
E. filter;
F. vacuum concentration,
F.1 temperature is 40 ℃～70 ℃,
F.2 vacuum tightness 85～98KPa,
F.3 time 30～90min;
G. ethanol sedimentation,
G.1 95% ethanol that adds 2～5 times of liquid concentrator volumes,
G.2 pure shallow lake time 4～15h;
It is h. dry,
H.140 ℃～70 ℃ of oven dry;
I. lentinan.
2. a kind of method of utilizing ultra-high voltage to extract lentinan according to claim 1 is characterized in that being undertaken by following concrete operations step:
A. select dry mushroom;
B. pulverize,
B.1 pulverized 20 orders;
C. add pure water,
C.1 the mushroom after will pulverizing adds in the pure water, and its quality proportioning is 1: 30;
D. uhp treatment,
D.1 pressure 400MPa,
D.2 temperature is 50 ℃,
D.3 dwell time 6min,
D.4 release 2 times repeat to boost;
E. filter;
F. vacuum concentration,
F.1 thickening temperature is 50 ℃,
F.2 vacuum tightness 85KPa,
F.3 concentration time 45min;
G. ethanol sedimentation,
G.1 95% ethanol that adds 3 times of liquid concentrator volumes,
G.2 pure shallow lake time 8h;
It is h. dry,
H.150 ℃ oven dry;
I. lentinan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101505332A CN101323649B (en) | 2008-08-04 | 2008-08-04 | Method for extracting mushroom polysaccharide by using ultra-high pressure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101505332A CN101323649B (en) | 2008-08-04 | 2008-08-04 | Method for extracting mushroom polysaccharide by using ultra-high pressure |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101323649A CN101323649A (en) | 2008-12-17 |
| CN101323649B true CN101323649B (en) | 2012-02-29 |
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| CN2008101505332A Expired - Fee Related CN101323649B (en) | 2008-08-04 | 2008-08-04 | Method for extracting mushroom polysaccharide by using ultra-high pressure |
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Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2353402A1 (en) | 2010-02-05 | 2011-08-10 | Sense for taste | Method for altering the flavor of a food product |
| CN102150823B (en) * | 2010-12-28 | 2012-11-28 | 浙江省农业科学院 | Method for improving micromeritic characteristics of agrocybe aegerita and improving dissolution of part of nutrient substances |
| CN102793148B (en) * | 2011-05-23 | 2014-07-16 | 杨志强 | Method for processing instant nutrition pholiota nameko by utilizing ultrahigh pressure |
| CN103570841A (en) * | 2012-07-26 | 2014-02-12 | 福建省安新食品有限公司 | High-efficiency extraction method for pleurotus geesteranus polysaccharide |
| CN103564420B (en) * | 2012-07-26 | 2016-01-20 | 福建省安新食品有限公司 | A kind of production technology of canned mushroom |
| CN103694365B (en) * | 2013-12-12 | 2017-08-25 | 四川好医生攀西药业有限责任公司 | A kind of technique of ultra high pressure extraction blazei polysaccharide |
| CN104744600B (en) * | 2015-04-09 | 2017-04-12 | 四川农业大学 | Process for performing high pressure combined ultrasound-associated extraction on active substance from mushroom |
| CN104892785B (en) * | 2015-05-29 | 2017-07-07 | 湖南尔康制药股份有限公司 | A kind of extracting method of algal polysaccharides |
| CN106420876A (en) * | 2016-11-16 | 2017-02-22 | 马业忠 | Drinking liquid with uric acid decreasing and lipid lowering effects and preparation method of drinking liquid |
| CN106674369A (en) * | 2016-12-21 | 2017-05-17 | 临沂信邦生物科技有限公司 | Preparation method of lentinan |
| CN109384859A (en) * | 2018-12-18 | 2019-02-26 | 大连理工大学 | A kind of superhigh pressure extracting method of Siberian solomonseal rhizome polysaccharide |
| CN110229245A (en) * | 2019-07-22 | 2019-09-13 | 玉林师范学院 | A kind of preparation method with activity of fighting against senium beautiful millettia root polysaccharide |
| CN111289666A (en) * | 2020-03-11 | 2020-06-16 | 无限极（中国）有限公司 | Construction method of lentinan multivariate quantitative fingerprint spectrum |
| CN113940420A (en) * | 2020-07-18 | 2022-01-18 | 哈尔滨腾凝科技有限公司 | Method for promoting release of mushroom flavor substances by using ultrahigh pressure wall breaking technology |
| CN111978429B (en) * | 2020-09-16 | 2022-07-19 | 上海交通大学 | Extraction method of flammulina velutipes root polysaccharide |
Citations (2)
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|---|---|---|---|---|
| KR20010025337A (en) * | 2000-12-16 | 2001-04-06 | 정종상 | A method of preparing the antitumor and immuno-activity polysaccharide from the artificial cultivation of inonotus obliquus, and its use |
| CN1765935A (en) * | 2005-11-04 | 2006-05-03 | 杨勇杰 | Method for separating lentinan |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010025337A (en) * | 2000-12-16 | 2001-04-06 | 정종상 | A method of preparing the antitumor and immuno-activity polysaccharide from the artificial cultivation of inonotus obliquus, and its use |
| CN1765935A (en) * | 2005-11-04 | 2006-05-03 | 杨勇杰 | Method for separating lentinan |
Non-Patent Citations (2)
| Title |
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| 朱俊杰等.破壁提取综合法提取灵芝孢子多糖的研究.《农业机械学报》.2004,第35卷(第3期),184-185. * |
| 朱俊洁等.破壁提取综合法提取灵芝孢子多糖的研究.《农业机械学报》.2004,第35卷(第3期),184-185. * |
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