CN101323649A

CN101323649A - Method for extracting mushroom polysaccharide by using ultra-high pressure

Info

Publication number: CN101323649A
Application number: CNA2008101505332A
Authority: CN
Inventors: 杜冰; 杨公明; 温升南; 唐建; 李德灵
Original assignee: NANFANG LEE KUM KEE CO Ltd
Current assignee: NANFANG LEE KUM KEE CO Ltd
Priority date: 2008-08-04
Filing date: 2008-08-04
Publication date: 2008-12-17
Anticipated expiration: 2028-08-04
Also published as: CN101323649B

Abstract

The invention discloses a method for extracting shiitake polysaccharides from shiitakes, which overcomes the defects that the existing extracting method requires long time, has low efficiency and high energy consumption and damages active ingredients of the shiitake polysaccharides. The proposal adopted by the invention comprises the following steps of: crushing the selected dry shiitakes, adding extract, processing under super-high pressure, filtering, concentrating in vacuum, precipitating with alcohol, drying and finally obtaining shiitake polysaccharides. The high pressure is adopted by the invention to hold or relieve pressure of the shiitake extract, increase the differences between osmotic pressures in and out of cells and change the structure of cells, thereby easily dissolving out effective ingredients, keeping the physiologically active ingredients unchanged, killing a part of microorganisms by high-pressure processing and finishing extraction and sterilization in one step. Being a cold extracting technique, the super-high-pressure extracting method generates no high temperature during the whole extraction process, shortens the extracting time to a few minutes, conserves the effective ingredients, and has significant economic benefits and broad prospect of market development.

Description

A kind of method of utilizing ultra-high voltage to extract lentinan

Technical field

The present invention relates to a kind of method of extracting lentinan from mushroom, specifically is a kind of method of utilizing ultra-high voltage to extract lentinan, belongs to field of medicinal materials processing.

Background technology

China is Edible Fungi big country, and wherein the output of mushroom occupies first place in the world.Mushroom contains rich in protein, nutritive ingredients such as amino acid, fat, carbohydrate, multivitamin and mineral substance, wherein the lentinan in the carbohydrate have antiviral, antitumor, regulate immunologic function and stimulate multinomial functions such as Interferon, rabbit formation.Lentinan is not only a kind of more satisfactory medicine, also is the important protective foods functional factor of a class, is current medicine and foodstuffs industry Focal Point of Common Attention, has the wide development application prospect.

At present, the most frequently used extracting method of lentinan is a water extraction method, and this method extraction time is long, efficient is low, energy consumption is big, has also destroyed the activeconstituents of lentinan simultaneously.In recent years, also the someone utilizes enzyme process, microwave method or ultrasonic extraction method lentinan, though these methods have improved polysaccharide yield to a certain extent, extraction time still was thirty minutes long 60 minutes, the cost height causes sex change inactivation, the radiation effect HUMAN HEALTH of effective constituent easily.Therefore, the extracting method of a kind of lentinan fast and effectively of exploitation has become the key of lentinan product development.Utilize the ultra-high voltage extractive technique to extract lentinan, at home and abroad do not appear in the newspapers as yet at present.

Summary of the invention

The objective of the invention is to disclose a kind of method of utilizing ultra-high voltage rapid extraction lentinan, overcome the defective that present multiple extraction lentinan method exists, constant with the activeconstituents that guarantees extraction back lentinan.

The present invention finishes by the following steps preparation:

A. select dry mushroom;

B. pulverize;

C. add extracting solution;

D. uhp treatment;

E. filter;

F. vacuum concentration;

G. ethanol sedimentation;

H. dry;

I. lentinan.

The present invention adopts high pressure that the mushroom feed liquid is carried out pressurize, release rapidly then, increase the inside and outside permeable pressure head of cell, the structure of cell is changed, thereby make its effective constituent be able to rapid stripping, ultrahigh-pressure extraction method is a kind of cold extraction technology, can not produce high temperature in whole leaching process, so kept the original physiologically active ingredient of mushroom constant, autoclaving has been killed the part microorganism simultaneously, make to extract with one step of sterilization and finish, extraction time can foreshorten to several minutes.

Embodiment

Concrete operations step of the present invention is as follows:

A. select dry mushroom

B. pulverize

C. add pure water

C.1 the mushroom after will pulverizing adds in the pure water, and its quality proportioning is 1: 1-50;

D. uhp treatment

D.1 pressure 100～600MPa

D.2 temperature is 20 ℃～80 ℃

D.3 dwell time 1～10min

D.4 release 1～3 time repeats to boost;

E. filter

F. vacuum concentration

F.1 temperature is 40 ℃～70 ℃

F.2 vacuum tightness 85～98KPa

F.3 time 30～90min;

G. ethanol sedimentation

G.1 95% ethanol that adds 2～5 times of concentrated solution volumes

G.2 pure shallow lake time 4～15h;

H. dry

H.140 ℃～70 ℃ of oven dry;

I. lentinan.

Now provide following examples, but the present invention is not limited to present embodiment:

A. select dry mushroom

B. pulverize

B.1 pulverized 20 orders;

C. add pure water

C.1 the mushroom after will pulverizing adds in the pure water, and its quality proportioning is 1: 30;

D. uhp treatment

D.1 pressure 400MPa

D.2 temperature is 50 ℃

D.3 dwell time 6min

D.4 release 2 times repeat to boost;

E. filter

F. vacuum concentration

F.1 thickening temperature is 50 ℃

F.2 vacuum tightness 85KPa

F.3 concentration time 45min;

G. ethanol sedimentation

G.1 95% ethanol that adds 3 times of concentrated solution volumes

G.2 pure shallow lake time 8h;

H. dry

H.150 ℃ oven dry;

I. lentinan.

The present invention has thoroughly solved the defective that present lentinan extracting method exists, and its technology is simple, polysaccharide yield is high, foreign matter content is few, cost is low.Can more effectively preserve the activeconstituents of lentinan, strengthen effect.A complete set of technology is simple, easy to operate, continuity is good, easily realizes industrialization, products obtained therefrom steady quality, safety.Solved the technology barrier that present lentinan extraction efficiency is low, cost is high, foreign matter content is high.Change the present situation of high-quality in the market lentinan rareness, realized the increment of lentinan.The present invention simultaneously has the huge economic benefit and the prospect of marketing also for the extraction of pharmaceutically active ingredient in other provides a kind of novel process fast and efficiently.

Claims

1. method of utilizing ultra-high voltage to extract lentinan is characterized in that finishing by the following steps preparation:

1.a select dry mushroom;

1.b pulverize;

1.c adding extracting solution;

1.d uhp treatment;

1.e filter;

1.f vacuum concentration;

1.g ethanol sedimentation;

1.h it is dry;

1.i lentinan.

2. a kind of method of utilizing ultra-high voltage to extract lentinan according to claim 1 is characterized in that being undertaken by following concrete operations step:

2.a select dry mushroom

2.b pulverize

2.c adding pure water

2.c.1 the mushroom after will pulverizing adds in the pure water, its quality proportioning is 1: 1-50;

2.d uhp treatment

2.d.1 pressure 100～600MPa

2.d.2 20 ℃～80 ℃ of temperature

2.d.3 dwell time 1～10min

Release is 1～3 time 2.d.4 repeat to boost;

2.e filter

2.f vacuum concentration

2.f.1 40 ℃～70 ℃ of temperature

2.f.2 vacuum tightness 85～98KPa

2.f.3 time 30～90min;

2.g ethanol sedimentation

2.g.1 add 95% ethanol of 2～5 times of concentrated solution volumes

2.g.2 pure shallow lake time 4～15h;

2.h it is dry

2.h.1 40 ℃～70 ℃ oven dry;

2.i lentinan.

3. a kind of method of utilizing ultra-high voltage to extract lentinan according to claim 1 is characterized in that being undertaken by following concrete operations step:

3.a select dry mushroom

3.b pulverize

3.b.1 pulverized 20 orders;

3.c adding pure water

3.c.1 the mushroom after will pulverizing adds in the pure water, its quality proportioning is 1: 30;

3.d uhp treatment

3.d.1 pressure 400MPa

3.d.2 50 ℃ of temperature

3.d.3 dwell time 6min

Release is 2 times 3.d.4 repeat to boost;

3.e filter

3.f vacuum concentration

3.f.1 50 ℃ of thickening temperatures

3.f.2 vacuum tightness 85KPa

3.f.3 concentration time 45min;

3.g ethanol sedimentation

3.g.1 add 95% ethanol of 3 times of concentrated solution volumes

3.g.2 pure shallow lake time 8h;

3.h it is dry

3.h.1 50 ℃ of oven dry;

3.i lentinan.