CN104194942A - Extracting method for rhizoma cyperi essential oil - Google Patents
Extracting method for rhizoma cyperi essential oil Download PDFInfo
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- CN104194942A CN104194942A CN201410445659.8A CN201410445659A CN104194942A CN 104194942 A CN104194942 A CN 104194942A CN 201410445659 A CN201410445659 A CN 201410445659A CN 104194942 A CN104194942 A CN 104194942A
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- rhizoma cyperi
- extracting method
- extracting
- essential oil
- volatile oil
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Abstract
The invention relates to an extracting method for rhizoma cyperi essential oil. The extracting method comprises the following treatment steps: getting and crushing rhizoma cyperi, passing the crushed rhizoma cyperi through a 40-meshed sieve, placing the sieved rhizoma cyperi into a fermentation barrel, based on the weight of raw materials, adding 0.5-1.0% of cellulase, 0.5-1.0% of hemicellulase and 0.5-1.0% of acid protease to uniform mix, adding buffer liquor, namely acetic acid/sodium acetate with pH of 4.5-5.5, until the mixture can be held into clusters by hands, sealing and fermenting for 8-10 days at 35-45 DEG C; taking out the fermented raw materials, extracting by virtue of a microwave process, continuously extracting for 1-2 hours under microwave power of 600-800W, filtering, extracting filtrate twice to three times by use of an organic solvent, combining the extracting liquor, drying by use of anhydrous sodium sulfate, and compressing under reduced pressure to obtain the rhizoma cyperi essential oil. According to the extracting method, the yield of the rhizoma cyperi essential oil is effectively increased by adopting a method of combining mixed enzyme natural fermentation with microwave extraction, the quality of the rhizoma cyperi essential oil is good, and thus, the extracting method is suitable for industrial production.
Description
Technical field
The present invention relates to a kind of extracting method of Rhizoma Cyperi volatile oil, belong to extracted form natural plant product technical field.
Background technology
Rhizoma cyperi is the dry rhizome of sedge nutgrass flatsedge, another name Rhizoma Cyperi, cyperi rhizoma, eastern rhizoma cyperi.Warm in nature, taste is pungent, bitter, micro-sweet, flat.Return liver, spleen, tri-jiao channel; Energy promoting qi circulation and removing obstruction in the collateral, menstruction regulating and pain relieving; For stagnation of QI due to depression of the liver, chest side of body abdominal distention, maldigestion, vexed, the cold hernia stomachache of chest gastral cavity ruffian, distending pain of the breast, menoxenia, through closing the diseases such as dysmenorrhoea.In rhizoma cyperi medicinal material, Rhizoma Cyperi volatile oil plays main pharmacodynamics effect, mainly contain cyperene, cyperol,
α-cyperone,
βthe large class of-cyperone etc. is to heat-labile compound.
Conventional extracting method has steam distillation and supercritical fluid extraction.Though steam distillation is simple for process, because heat-up time is long, extract temperature higher, effective constituent is easily destroyed, and the volatile oil yield that extracts is low, impurity is many; And the extraction of supercutical fluid to volatile oil component, its have activity substance content high, do not have the advantages such as pest is residual, environmental protection, but this method is to the having relatively high expectations of extraction equipment, and is not suitable for suitability for industrialized production.
By literature search, have no identical with the present invention open source literature report.
Summary of the invention
The object of the present invention is to provide a kind of method of extracting volatile oil from rhizoma cyperi that yield is high and product quality is good.
The present invention includes following steps:
Getting rhizoma cyperi pulverizes, cross 40 mesh sieves, be placed in mash-back, add the cellulase, 0.5 ~ 1.0% hemicellulase of raw material weight 0.5 ~ 1.0%, 0.5 ~ 1.0% aspartic protease, mix, add the buffered soln of the acetic acid/sodium-acetate of pH4.5 ~ 5.5, reach hold agglomerating, sealing, in 35 ~ 45 ℃ of fermentation 8 ~ 10d; Raw material after fermentation is taken out, 20 ~ 50% the ethanolic soln that adds 4 ~ 6 times, adopt microwave method to extract, under the condition of microwave power 600 ~ 800W, extract continuously 1 ~ 2h, filter, concentrated, by organic solvent extraction 2 ~ 3 times of concentrated solution liquid, combining extraction liquid, anhydrous sodium sulfate drying, concentrating under reduced pressure, obtains Rhizoma Cyperi volatile oil.
The activity of cellulase of the present invention and hemicellulase is 30 ~ 60u/mg.
The activity of acid starch enzyme of the present invention is 300000 ~ 400000u/mg.
Organic solvent of the present invention is a kind of in sherwood oil, normal hexane or ether.
Technique of the present invention is simple, utilizes mixed enzyme to adopt aging process to extract Rhizoma Cyperi volatile oil in conjunction with microwave extraction technology, has effectively improved the extraction yield of rhizoma cyperi essential oil, and product quality is good, is applicable to suitability for industrialized production.
Below in conjunction with embodiment, further illustrate the present invention, but the scope of protection of present invention is not limited to following embodiment.
embodiment:
Embodiment 1:
Getting rhizoma cyperi pulverizes, cross 40 mesh sieves, be placed in mash-back, add the cellulase (active for 30u/mg), 1.0% hemicellulase (active is 30u/mg) of raw material weight 1.0%, 0.5% aspartic protease (active is 300000u/mg), mix, add the buffered soln of acetic acid/sodium-acetate of pH5.5, reach hold agglomerating, sealing, in 35 ℃ of fermentation 10d; Raw material after fermentation is taken out, 20% the ethanolic soln that adds 4 times, adopts microwave method to extract, and extracts continuously 1h under the condition of microwave power 800W, filter, filtrate is concentrated, by concentrated solution petroleum ether extraction 2 times, combining extraction liquid, anhydrous sodium sulfate drying, concentrating under reduced pressure, obtains Rhizoma Cyperi volatile oil, and yield is 2.1%.
Embodiment 2:
Getting rhizoma cyperi pulverizes, cross 40 mesh sieves, be placed in mash-back, add the cellulase (active for 60u/mg), 0.5% hemicellulase (active is 60u/mg) of raw material weight 1.0%, 1.0% aspartic protease (active is 400000u/mg), mix, add the buffered soln of acetic acid/sodium-acetate of pH4.5, reach hold agglomerating, sealing, in 45 ℃ of fermentation 9d; Raw material after fermentation is taken out, 40% the ethanolic soln that adds 5 times, adopts microwave method to extract, and extracts continuously 2h under the condition of microwave power 700W, filter, filtrate is concentrated, by concentrated solution n-hexane extraction 2 times, combining extraction liquid, anhydrous sodium sulfate drying, concentrating under reduced pressure, obtains Rhizoma Cyperi volatile oil, and yield is 2.5%.
Embodiment 3:
Getting rhizoma cyperi pulverizes, cross 40 mesh sieves, be placed in mash-back, add the cellulase (active for 50u/mg), 0.5% hemicellulase (active is 50u/mg) of raw material weight 0.5%, 1.0% aspartic protease (active is 400000u/mg), mix, add the buffered soln of acetic acid/sodium-acetate of pH5.0, reach hold agglomerating, sealing, in 40 ℃ of fermentation 8d; Raw material after fermentation is taken out, 50% the ethanolic soln that adds 6 times, adopts microwave method to extract, and extracts continuously 2h under the condition of microwave power 600W, filter, filtrate is concentrated, by concentrated solution extracted with diethyl ether 2 times, combining extraction liquid, anhydrous sodium sulfate drying, concentrating under reduced pressure, obtains Rhizoma Cyperi volatile oil, and yield is 2.2%.
Claims (4)
1. the extracting method of a Rhizoma Cyperi volatile oil, it is characterized in that, comprise the following steps: get rhizoma cyperi and pulverize, cross 40 mesh sieves, be placed in mash-back, add the cellulase, 0.5 ~ 1.0% hemicellulase of raw material weight 0.5 ~ 1.0%, 0.5 ~ 1.0% aspartic protease, mix, add the buffered soln of the acetic acid/sodium-acetate of pH4.5 ~ 5.5, reach hold agglomerating, sealing, in 35 ~ 45 ℃ of fermentation 8 ~ 10d; Raw material after fermentation is taken out, 20 ~ 50% the ethanolic soln that adds 4 ~ 6 times, adopt microwave method to extract, under the condition of microwave power 600 ~ 800W, extract continuously 1 ~ 2h, filter, concentrated, by organic solvent extraction 2 ~ 3 times of concentrated solution liquid, combining extraction liquid, anhydrous sodium sulfate drying, concentrating under reduced pressure, obtains Rhizoma Cyperi volatile oil.
2. the extracting method of a kind of Rhizoma Cyperi volatile oil according to claim 1, is characterized in that, described cellulase and the activity of hemicellulase are 30 ~ 60u/mg.
3. the extracting method of a kind of Rhizoma Cyperi volatile oil according to claim 1, is characterized in that, the activity of described acid starch enzyme is 300000 ~ 400000u/mg.
4. the extracting method of a kind of Rhizoma Cyperi volatile oil according to claim 1, is characterized in that, described organic solvent is a kind of in sherwood oil, normal hexane or ether.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410445659.8A CN104194942A (en) | 2014-09-04 | 2014-09-04 | Extracting method for rhizoma cyperi essential oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410445659.8A CN104194942A (en) | 2014-09-04 | 2014-09-04 | Extracting method for rhizoma cyperi essential oil |
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| Publication Number | Publication Date |
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| CN104194942A true CN104194942A (en) | 2014-12-10 |
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| CN201410445659.8A Pending CN104194942A (en) | 2014-09-04 | 2014-09-04 | Extracting method for rhizoma cyperi essential oil |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107252432A (en) * | 2017-07-19 | 2017-10-17 | 辽宁华润本溪三药有限公司 | A kind of pharmaceutical composition for promoting gastroenteritic power and preparation method thereof and purposes |
| CN109744262A (en) * | 2018-11-26 | 2019-05-14 | 新昌县天姥园艺发展有限公司 | The preparation method of sweet viburnum cuttage root-taking agent |
-
2014
- 2014-09-04 CN CN201410445659.8A patent/CN104194942A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107252432A (en) * | 2017-07-19 | 2017-10-17 | 辽宁华润本溪三药有限公司 | A kind of pharmaceutical composition for promoting gastroenteritic power and preparation method thereof and purposes |
| CN109744262A (en) * | 2018-11-26 | 2019-05-14 | 新昌县天姥园艺发展有限公司 | The preparation method of sweet viburnum cuttage root-taking agent |
| CN109744262B (en) * | 2018-11-26 | 2021-07-09 | 何妮妮 | Preparation method of coral tree cutting rooting agent |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141210 |
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| WD01 | Invention patent application deemed withdrawn after publication |