CN105624252A - Saussurea involucrate micro-molecular peptide extract as well as preparation method and application thereof - Google Patents
Saussurea involucrate micro-molecular peptide extract as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of a traditional Chinese medicine extraction method and particularly provides a saussurea involucrate micro-molecular peptide extract and application thereof. The saussurea involucrate micro-molecular peptide extract is prepared by the following steps: drying, crushing, primary crude extraction, secondary crude extraction, enzymolysis, decoloration, concentration and drying. According to a preparation method of the saussurea involucrate micro-molecular peptide extract, provided by the invention, various beneficial active ingredients in saussurea involucrate can be fully extracted, and the extraction rate of the beneficial active ingredients is high. The molecular weights of micro-molecular peptides in the saussurea involucrate micro-molecular peptide extract are mainly distributed between 100-1000 D, and the micro-molecular peptides mostly are dipeptide, tripeptide, tetrapeptide and the like, belong to micro-molecular structures and have the characteristic of being very easy to absorb and utilize by human bodies.
Description
Technical field
The invention belongs to Chinese medicinal materials extracting method technical field, it is specifically related to a kind of Herba Saussureae Involueratae small-molecular peptides extract, its preparation method and application.
Background technology
Snow Lotus Herb (formal name used at school: Saussureainvolucrata) mainly refers to that composite family hieracioides belongs to fleshiness plant, has another name called Herba Saussureae Involueratae, Herba Saussureae Involueratae, Herba Saussureae Involueratae. It is exotic flowers and rare herbs rare to see. Xinjiang, the Tibet region people are referred to as Ka Erlaili, high 15-35 centimetre, can do medicinal, also have certain ornamental value. Tibetan language claims just really to revive bar, and Uighur claims Ka Erguli. It is born in hillside, mountain valley, crack of stone, waterside, grassy marshland, height above sea level 2400-3470 rice more. Russia and in state-owned distribution. Herba Saussureae Involueratae belongs to precious medical flower plant, containing volatile oil, alkaloid, flavonoid, phenols, carbohydrate, tannin isoreactivity composition, has except cold phlegm, tonifying yang and enriching blood, and the loose stasis of blood in warm palace, controls the effects such as menoxenia. In addition, Herba Saussureae Involueratae is to treatment lumbago due to renal deficiency, and wind-damp dispelling, the disease such as restore menstrual flow and invigorate blood circulation has positive effect.
Medicinal and the health care great in view of Herba Saussureae Involueratae is worth, and in industry, the extraction of its effective constituent has been done more further investigation. Nonetheless, the deficiencies such as the Herba Saussureae Involueratae extract that traditional extracting method obtains still exists that effective component extraction yield is low, gained Herba Saussureae Involueratae extract drug effect and health-care effect difference.
Summary of the invention
In order to solve the problems referred to above that prior art exists, the present invention provides the Herba Saussureae Involueratae small-molecular peptides extract extracting method of the effective component extraction yield height of a kind of Herba Saussureae Involueratae and the extract health effect excellence of gained. Meanwhile, the present invention also provides the Herba Saussureae Involueratae small-molecular peptides extract and application thereof of preparing gained accordingly.
The technical solution adopted in the present invention is:
First the present invention provides the preparation method of a kind of Herba Saussureae Involueratae small-molecular peptides extract, comprises the steps:
S1 is dry: Herba Saussureae Involueratae is dried process;
S2 pulverizes: dried Herba Saussureae Involueratae is carried out pulverization process;
S3 once slightly carries: is mixed with water by the Herba Saussureae Involueratae after pulverizing, is heated to backflow and once slightly carries;
S4 slightly carries for bis-times: after once slightly carrying, add methyl alcohol, ultrasonic carry out two times and slightly carries;
S5 enzymolysis: after slightly carrying for two times, adds proteolytic enzyme and carries out enzymolysis, and enzymolysis is complete obtains enzymolysis solution;
S6 decolours: filters enzymolysis solution and gets filtrate, adds bleaching agent bleaching in filtrate, after decolouring, filters and gets filtrate;
S7 concentrates: the filtrate of gained after vacuum concentration decolorization filtering, obtains extract crude product;
S8 is dry: extract crude product described in lyophilize obtains described Herba Saussureae Involueratae extract.
Further advantageous embodiment, in S1 after drying treatment, the moisture weight part content in described Herba Saussureae Involueratae is not more than 5%.
Further advantageous embodiment, pulverizes into particle diameter 90 object particle by dried Herba Saussureae Involueratae in S2.
Further advantageous embodiment, the weight ratio of Herba Saussureae Involueratae described in S3 and water is 1:10, and once slightly carrying middle reflux extracting time is 2 hours.
Further advantageous embodiment, the weight of the methyl alcohol added in S4 is 3 times of dried Herba Saussureae Involueratae weight, slightly carries for described two times and is specially under 70 DEG C of conditions supersound extraction 1.2 hours.
Further advantageous embodiment, described in S5, enzymolysis specifically comprises the following steps:
The pH of system is adjusted to 9.4 by the aqueous sodium hydroxide solution of S5.1 functional quality mark 15%, add Sumizyme MP, enzymolysis 1 hour under 60 DEG C of conditions, the add-on of described Sumizyme MP is 0.02 times of Herba Saussureae Involueratae weight after described drying, and the activity of described Sumizyme MP is 500,000 u/g;
The pH of system is adjusted to 7.2 by the aqueous sodium hydroxide solution of S5.2 functional quality mark 15% and the hydrochloric acid of massfraction 8%, add neutral protease, enzymolysis 1 hour under 60 DEG C of conditions, the add-on of described neutral protease is 0.01 times of Herba Saussureae Involueratae weight after described drying, and the activity of described Sumizyme MP is 150,000 u/g;
The pH of system is adjusted to 3.2 by the hydrochloric acid of S5.3 functional quality mark 8%, add stomach en-, enzymolysis 5 hours under 65 DEG C of conditions, the add-on of described neutral protease is 0.01 times of Herba Saussureae Involueratae weight after described drying, and the activity of described Sumizyme MP is 50,000 u/g;
System is warming up to backflow by S5.4, and backflow insulation 30 minutes, enzymolysis terminates.
Further advantageous embodiment, discoloring agent described in S6 is gac.
Further advantageous embodiment, in S7, the temperature of vacuum concentration is 60 DEG C, and after concentrated, the volume of the extract crude product obtained is 1/5th of the volume of concentrated front filtrate.
Secondly, present invention also offers the Herba Saussureae Involueratae small-molecular peptides extract prepared by aforementioned preparation process, the small-molecular peptides molecular weight of described Herba Saussureae Involueratae small-molecular peptides extract is mainly distributed between 100-1000D, it mostly is the small-molecular peptides such as dipeptides, tripeptides, four peptides, belong to small molecule structure, have the advantages that very easily be absorbed by the body and utilize. Herba Saussureae Involueratae small-molecular peptides extract provided by the invention is also containing other a large amount of nutritional benefit compositions, comprise volatile oil, alkaloid, flavonoid, phenols, carbohydrate, tannin isoreactivity composition, growth in humans can not only be provided to grow required nutritive substance, and there is unique biological function.
Meanwhile, accordingly, present invention also offers the Herba Saussureae Involueratae small-molecular peptides extract of aforementioned the present invention in the application prepared in healthcare products and Chinese herbal product.
Discussing based on above scheme, the useful effect of the present invention is: the preparation method of Herba Saussureae Involueratae small-molecular peptides extract provided by the invention can fully extract the various beneficial activity compositions in Herba Saussureae Involueratae, and beneficial activity constituents extraction rate height. The small-molecular peptides molecular weight of the Herba Saussureae Involueratae small-molecular peptides extract of the present invention is mainly distributed between 100-1000D, mostly is the small-molecular peptides such as dipeptides, tripeptides, four peptides, belongs to small molecule structure, has the advantages that very easily be absorbed by the body and utilize.
Embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated.
Embodiment 1:
Present embodiments provide a kind of Herba Saussureae Involueratae small-molecular peptides extract.
The preparation method of the Herba Saussureae Involueratae small-molecular peptides extract of the present embodiment comprises the following steps:
S1 is dry: Herba Saussureae Involueratae is dried process;
S2 pulverizes: dried Herba Saussureae Involueratae is carried out pulverization process;
S3 once slightly carries: is mixed with water by the Herba Saussureae Involueratae after pulverizing, is heated to backflow and once slightly carries;
S4 slightly carries for bis-times: after once slightly carrying, add methyl alcohol, ultrasonic carry out two times and slightly carries;
S5 enzymolysis: after slightly carrying for two times, adds proteolytic enzyme and carries out enzymolysis, and enzymolysis is complete obtains enzymolysis solution;
S6 decolours: filters enzymolysis solution and gets filtrate, adds bleaching agent bleaching in filtrate, after decolouring, filters and gets filtrate;
S7 concentrates: the filtrate of gained after vacuum concentration decolorization filtering, obtains extract crude product;
S8 is dry: extract crude product described in lyophilize obtains described Herba Saussureae Involueratae extract.
Meanwhile, the present embodiment additionally provides the Herba Saussureae Involueratae small-molecular peptides extract obtained by the present embodiment in the application prepared in healthcare products and Chinese herbal product.
Embodiment 2:
The present embodiment 2 also provides a kind of Herba Saussureae Involueratae small-molecular peptides extract.
The preparation method of the Herba Saussureae Involueratae small-molecular peptides extract of the present embodiment 2 is the further Optimal improvements on embodiment 1 basis, and concrete optimization details is as follows:
In S1 after drying treatment, the moisture weight part content in described Herba Saussureae Involueratae is not more than 5%. In three examples arranged side by side of the present embodiment, after dry, the moisture weight part content in described Herba Saussureae Involueratae is respectively 1%, 2% and 5%.
Dried Herba Saussureae Involueratae is pulverized into particle diameter 90 object particle by S2.
The weight ratio of Herba Saussureae Involueratae described in S3 and water is 1:10, and once slightly carrying middle reflux extracting time is 2 hours.
The weight of the methyl alcohol added in S4 is 3 times of dried Herba Saussureae Involueratae weight, slightly carries for described two times and is specially under 70 DEG C of conditions supersound extraction 1.2 hours.
Described in S5, enzymolysis specifically comprises the following steps: the pH of system is adjusted to 9.4 by the aqueous sodium hydroxide solution of S5.1 functional quality mark 15%, add Sumizyme MP, enzymolysis 1 hour under 60 DEG C of conditions, the add-on of described Sumizyme MP is 0.02 times of Herba Saussureae Involueratae weight after described drying, and the activity of described Sumizyme MP is 500,000 u/g; The pH of system is adjusted to 7.2 by the aqueous sodium hydroxide solution of S5.2 functional quality mark 15% and the hydrochloric acid of massfraction 8%, add neutral protease, enzymolysis 1 hour under 60 DEG C of conditions, the add-on of described neutral protease is 0.01 times of Herba Saussureae Involueratae weight after described drying, and the activity of described Sumizyme MP is 150,000 u/g; The pH of system is adjusted to 3.2 by the hydrochloric acid of S5.3 functional quality mark 8%, add stomach en-, enzymolysis 5 hours under 65 DEG C of conditions, the add-on of described neutral protease is 0.01 times of Herba Saussureae Involueratae weight after described drying, and the activity of described Sumizyme MP is 50,000 u/g; System is warming up to backflow by S5.4, and backflow insulation 30 minutes, enzymolysis terminates.
Discoloring agent described in S6 is gac.
In S7, the temperature of vacuum concentration is 60 DEG C, and after concentrated, the volume of the extract crude product obtained is 1/5th of the volume of concentrated front filtrate.
The Herba Saussureae Involueratae small-molecular peptides extract that the present embodiment 2 prepares gained can be used for preparing healthcare products and Chinese herbal product equally.
The present invention is not limited to above-mentioned preferred forms; anyone can draw the product of other various forms under the enlightenment of the present invention; though but in its details, do any change, every have the technical scheme identical or close with the application, all drops within protection scope of the present invention.
Claims (10)
1. the preparation method of Herba Saussureae Involueratae small-molecular peptides extract, it is characterised in that, comprise the following steps:
S1 is dry: Herba Saussureae Involueratae is dried process;
S2 pulverizes: dried Herba Saussureae Involueratae is carried out pulverization process;
S3 once slightly carries: is mixed with water by the Herba Saussureae Involueratae after pulverizing, is heated to backflow and once slightly carries;
S4 slightly carries for bis-times: after once slightly carrying, add methyl alcohol, ultrasonic carry out two times and slightly carries;
S5 enzymolysis: after slightly carrying for two times, adds proteolytic enzyme and carries out enzymolysis, and enzymolysis is complete obtains enzymolysis solution;
S6 decolours: filters enzymolysis solution and gets filtrate, adds bleaching agent bleaching in filtrate, after decolouring, filters and gets filtrate;
S7 concentrates: the filtrate of gained after vacuum concentration decolorization filtering, obtains extract crude product;
S8 is dry: extract crude product described in lyophilize obtains described Herba Saussureae Involueratae extract.
2. the preparation method of Herba Saussureae Involueratae small-molecular peptides extract according to claim 1, it is characterised in that: in S1 after drying treatment, the moisture weight part content in described Herba Saussureae Involueratae is not more than 5%.
3. the preparation method of Herba Saussureae Involueratae small-molecular peptides extract according to claim 1, it is characterised in that: dried Herba Saussureae Involueratae is pulverized into particle diameter 90 object particle by S2.
4. the preparation method of Herba Saussureae Involueratae small-molecular peptides extract according to claim 1, it is characterised in that: the weight ratio of Herba Saussureae Involueratae described in S3 and water is 1:10, and once slightly carrying middle reflux extracting time is 2 hours.
5. the preparation method of Herba Saussureae Involueratae small-molecular peptides extract according to claim 1, it is characterized in that: the weight of the methyl alcohol added in S4 is 3 times of dried Herba Saussureae Involueratae weight, slightly carry for described two times and be specially under 70 DEG C of conditions supersound extraction 1.2 hours.
6. the preparation method of Herba Saussureae Involueratae small-molecular peptides extract according to claim 1, it is characterised in that:
Described in S5, enzymolysis specifically comprises the following steps:
The pH of system is adjusted to 9.4 by the aqueous sodium hydroxide solution of S5.1 functional quality mark 15%, add Sumizyme MP, enzymolysis 1 hour under 60 DEG C of conditions, the add-on of described Sumizyme MP is 0.02 times of Herba Saussureae Involueratae weight after described drying, and the activity of described Sumizyme MP is 500,000 u/g;
The pH of system is adjusted to 7.2 by the aqueous sodium hydroxide solution of S5.2 functional quality mark 15% and the hydrochloric acid of massfraction 8%, add neutral protease, enzymolysis 1 hour under 60 DEG C of conditions, the add-on of described neutral protease is 0.01 times of Herba Saussureae Involueratae weight after described drying, and the activity of described Sumizyme MP is 150,000 u/g;
The pH of system is adjusted to 3.2 by the hydrochloric acid of S5.3 functional quality mark 8%, add stomach en-, enzymolysis 5 hours under 65 DEG C of conditions, the add-on of described neutral protease is 0.01 times of Herba Saussureae Involueratae weight after described drying, and the activity of described Sumizyme MP is 50,000 u/g;
System is warming up to backflow by S5.4, and backflow insulation 30 minutes, enzymolysis terminates.
7. the preparation method of Herba Saussureae Involueratae small-molecular peptides extract according to claim 1, it is characterised in that: discoloring agent described in S6 is gac.
8. the preparation method of Herba Saussureae Involueratae small-molecular peptides extract according to claim 1, it is characterised in that: in S7, the temperature of vacuum concentration is 60 DEG C, and after concentrated, the volume of the extract crude product obtained is 1/5th of the volume of concentrated front filtrate.
9. Herba Saussureae Involueratae small-molecular peptides extract, it is characterised in that: obtain by the arbitrary described preparation method preparation of claim 1��8.
10. Herba Saussureae Involueratae small-molecular peptides extract described in claim 9 is in the application prepared in healthcare products and Chinese herbal product.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108113926A (en) * | 2017-12-29 | 2018-06-05 | 吉林农业科技学院 | A kind of Herba Saussureae Involueratae ultrasonic extraction process |
| CN108410937A (en) * | 2018-04-24 | 2018-08-17 | 烟台金蕊女性用品有限公司 | Herba Saussureae Involueratae peptide molecule extraction process |
| CN108546726A (en) * | 2018-03-22 | 2018-09-18 | 许昌学院 | A kind of lotus leaf polypeptide preparation process |
| CN108977483A (en) * | 2018-04-24 | 2018-12-11 | 许昌学院 | A kind of technique that papain prepares lotus leaf polypeptide |
| CN108998487A (en) * | 2018-04-24 | 2018-12-14 | 许昌学院 | A kind of technique that alkali protease prepares lotus leaf polypeptide |
| CN113717250A (en) * | 2021-11-02 | 2021-11-30 | 烟台金蕊女性用品有限公司 | Saussurea involucrate micromolecule polypeptide, extraction process and application thereof |
| CN113730430A (en) * | 2021-08-23 | 2021-12-03 | 无限极（中国）有限公司 | Application of small molecular saussurea involucrate polysaccharide in adiponectin secretion regulation and preparation method thereof |
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| CN101818155A (en) * | 2009-12-22 | 2010-09-01 | 石河子大学 | Application of herba saussureae involucratae sikDh2 gene in cultivating cold resistant plant |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108113926A (en) * | 2017-12-29 | 2018-06-05 | 吉林农业科技学院 | A kind of Herba Saussureae Involueratae ultrasonic extraction process |
| CN108546726A (en) * | 2018-03-22 | 2018-09-18 | 许昌学院 | A kind of lotus leaf polypeptide preparation process |
| CN108410937A (en) * | 2018-04-24 | 2018-08-17 | 烟台金蕊女性用品有限公司 | Herba Saussureae Involueratae peptide molecule extraction process |
| CN108977483A (en) * | 2018-04-24 | 2018-12-11 | 许昌学院 | A kind of technique that papain prepares lotus leaf polypeptide |
| CN108998487A (en) * | 2018-04-24 | 2018-12-14 | 许昌学院 | A kind of technique that alkali protease prepares lotus leaf polypeptide |
| CN113730430A (en) * | 2021-08-23 | 2021-12-03 | 无限极（中国）有限公司 | Application of small molecular saussurea involucrate polysaccharide in adiponectin secretion regulation and preparation method thereof |
| CN113730430B (en) * | 2021-08-23 | 2023-09-05 | 无限极（中国）有限公司 | Application of small molecule saussurea involucrata polysaccharide in adiponectin secretion regulation and preparation method thereof |
| CN113717250A (en) * | 2021-11-02 | 2021-11-30 | 烟台金蕊女性用品有限公司 | Saussurea involucrate micromolecule polypeptide, extraction process and application thereof |
| CN113717250B (en) * | 2021-11-02 | 2022-02-18 | 烟台金蕊女性用品有限公司 | Saussurea involucrate micromolecule polypeptide, extraction process and application thereof |
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