CN103446022A - Plant polypeptide protein extract and use thereof - Google Patents
Plant polypeptide protein extract and use thereof Download PDFInfo
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- CN103446022A CN103446022A CN2013103935872A CN201310393587A CN103446022A CN 103446022 A CN103446022 A CN 103446022A CN 2013103935872 A CN2013103935872 A CN 2013103935872A CN 201310393587 A CN201310393587 A CN 201310393587A CN 103446022 A CN103446022 A CN 103446022A
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Abstract
The invention provides a plant polypeptide protein extract and use thereof. The extract is prepared from the following steps: taking one of saussurea involucrata membranous bract, red coix seed containing tertia, rose petals, barbados aloe, lavender petals, fruits of tea plants, seaweed leaves, zante currant and chamomile petals as a plant raw material; firstly, coarsely crushing and then crushing to obtain a pre-treatment mixture of the raw material; hermetically soaking the pre-treatment mixture in deionized water at 80-85 DEG C for 10-15 minutes; then adjusting the pH value to 6.2-6.8, adding protease and cooling to carry out hydrolysis reaction; centrifuging, participating, filtering, stewing and layering, so as to obtain clear liquid; carrying out shielding protection on a polypeptide protein end group in the clear liquid; desalting and concentrating to obtain the plant polypeptide protein extract. The plant polypeptide protein extract can be used as a cell activity factor and applied to foods, health-care drugs and cosmetics. The plant polypeptide protein extract has the advantages of being high in extraction efficiency, stable in extract, difficult to deteriorate, difficult to lose activity.
Description
Technical field
The present invention relates to Plant Extracts and uses thereof, particularly, relate to a kind of plant polypeptide protein extract and uses thereof.
Background technology
Level raising along with the plant extract technology, extract in plant is utilized more and more, in numerous extracts, there is the material that a class contains polypeptide protein more and more to be paid attention to by people, because polypeptide protein is confirmed gradually to the cell beauty functions of human body skin, the application of cosmetic industry polypeptide protein cell active factor is more and more extensive, the polypeptide protein that different plants contains has different performances, also shows that the effect of beauty treatment has difference.
Prior art is when being extracted plant polypeptide albumen, and the following shortcoming and defect of existence: extract yield is low, and the product stability after extraction is poor, apt to deteriorate, and loss of activity is serious.
Based on this, according to studying for a long period of time and clinical experience, be necessary above shortcoming is improved, thereby a kind of new plant polypeptide protein extract and uses thereof is provided.
Summary of the invention
The object of the invention is to overcome the defect of above-mentioned prior art existence and a kind of plant polypeptide protein extract and uses thereof is provided, it is high that plant polypeptide protein extract provided by the invention has extraction efficiency, extract stability is high, not perishable, and active being difficult for loses.
First purpose of the present invention is achieved through the following technical solutions, a kind of plant polypeptide protein extract, and described extract obtains by following steps:
Step (1) is got the membranous bract of Herba Saussureae Involueratae, a kind of as plant material containing in the red Semen Coicis of rough bark, roseleaf, Aloe, lavandula angustifolia petal, Camellia sinensis fruit, Sargassum leaf, Zante currant, Flos Matricariae chamomillae petal, first coarse crushing, pulverize again, obtain the pretreatment mixture of plant material;
Step (2) is got the described pretreatment mixture of 3.0～5.0 weight portion and is soaked in 5.0～7.0 parts by weight of deionized water, then at 80～85 ℃ of lower seals, keeps 10～15 minutes, obtains and soaks mixed liquor;
The pH value that step (3) is regulated described immersion mixed liquor is 6.2～6.8, add protease, described in described protease and step (2), the mass ratio of pretreatment mixture is 0.1～1.0%, then cool under 40～45 ℃ the reaction 4～5h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is carried out shielding protection by acetylization reaction and/or mixed anhydride method reaction to the polypeptide protein end group in described clear liquid again, then carries out desalination, concentrated, obtains.
Preferably, in step (2), described pretreatment mixture is 4.0 weight portions, and described deionized water is 6.0 weight portions.
Preferably, in step (2), at 82～83 ℃ of lower seals, keep 12～13 minutes, obtain described immersion mixed liquor.
Preferably, in step (3), described pH value is 6.4～6.6, and the mass ratio of described protease and described pretreatment mixture is 0.3～0.8%.
Preferably, in step (3), cool under 42～43 ℃ the reaction 4～5h that is hydrolyzed.
Preferably, described acetylization reaction comprises following concrete steps: by described clear liquid, with glacial acetic acid, according to mass ratio, be 1: (1.1～1.3) are mixed, with [MORBSA] [HSO
4] ionic liquid carries out acetylization reaction as catalyst, described catalyst amount account for described clear liquid and described glacial acetic acid gross mass 0.6～0.9%, reaction separates described catalyst after finishing.Preferred, the mass ratio of described clear liquid and glacial acetic acid is 1: 1.2, described catalyst amount account for described clear liquid and described glacial acetic acid gross mass 0.7～0.8%.
Preferably, described mixed anhydride method reaction is used chloro-carbonic acid isopropyl esters and/or chloro-carbonic acid isobutyl to be reacted, described in described chloro-carbonic acid isopropyl esters and step (3), the mass ratio of clear liquid is 1: (1.3～1.5), the mass ratio 1 of clear liquid described in described chloro-carbonic acid isobutyl and step (3): (1.3～1.5).Preferred, described in described chloro-carbonic acid isopropyl esters and step (3), the mass ratio of clear liquid is 1: 1.4, the mass ratio of clear liquid described in described chloro-carbonic acid isobutyl and step (3) 1: 1.4.
The present invention also provides above-mentioned plant polypeptide protein extract to be used as the application of cell active factor in cosmetics.
The present invention is by selected plant hydrolyzed or multistage hydrolysis, then decomposes through specific protease, then through acetylation or/and amidatioon shielding strand end group, then through centrifugal, precipitation, filter, the technique such as concentrated, obtain needed plant polypeptide protein extract.
Compared with prior art, the present invention has following beneficial effect:
(1) extraction efficiency of the present invention is high, and the product after extraction is stable, can avoid unstable, the defect that the maintenance phase is short of polypeptide protein;
(2) technique of the present invention simple, drop into lowly, by adopting specific protease and end group protection, kept the effect of product, improved the stability of storage, be suitable for large-scale industrial production, can be widely used in the fields such as food, health care medicine, cosmetics;
(3) raw material is taken from natural plants, and not containing chemical additive, safety has no side effect, and product and by-product can be to human body and environments;
(4) the present invention, by precisely controlling the polypeptide peptide bond, makes the extract of producing retain the nutritional labeling in the plant.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.Following examples will contribute to those skilled in the art further to understand the present invention, but not limit in any form the present invention.It should be pointed out that to those skilled in the art, without departing from the inventive concept of the premise, can also make some distortion and improvement.These all belong to protection scope of the present invention.
embodiment 1,
The present embodiment relates to a kind of plant polypeptide protein extract, by following steps, obtains:
Step (1) is got the membranous bract of Herba Saussureae Involueratae as plant material, first coarse crushing, then pulverize, obtain the pretreatment mixture of plant material;
Step (2) is got 3.0 weight portion pretreatment mixture and is soaked in 7.0 parts by weight of deionized water, then at 80 ℃ of lower seals, keeps 15 minutes, obtains and soaks mixed liquor;
It is 6.2 that step (3) is regulated the pH value that soaks mixed liquor, adds protease, and in protease and step (2), the mass ratio of pretreatment mixture is 1.0%, then cool under 40 ℃ the reaction 5h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is mixed clear liquid according to mass ratio with glacial acetic acid at 1: 1.1, with [MORBSA] [HSO
4] ionic liquid carries out acetylization reaction as catalyst, by acetylization reaction, the polypeptide protein end group in clear liquid carried out to shielding protection, catalyst amount account for clear liquid and glacial acetic acid gross mass 0.9%; after reaction finishes; separate described catalyst, then carry out desalination, concentrated, obtain.
embodiment 2,
The present embodiment relates to a kind of plant polypeptide protein extract, by following steps, obtains:
Step (1) is got containing the red Semen Coicis of rough bark as plant material, first coarse crushing, then pulverize, obtain the pretreatment mixture of plant material;
Step (2) is got 5.0 weight portion pretreatment mixture and is soaked in 5.0 parts by weight of deionized water, then at 85 ℃ of lower seals, keeps 10 minutes, obtains and soaks mixed liquor;
It is 6.8 that step (3) is regulated the pH value that soaks mixed liquor, adds protease, and in protease and step (2), the mass ratio of pretreatment mixture is 0.1%, then cool under 45 ℃ the reaction 4h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is mixed clear liquid according to mass ratio with glacial acetic acid at 1: 1.3, with [MORBSA] [HSO
4] ionic liquid carries out acetylization reaction as catalyst, by acetylization reaction, the polypeptide protein end group in clear liquid carried out to shielding protection, catalyst amount account for clear liquid and glacial acetic acid gross mass 0.6%; after reaction finishes; separate described catalyst, then carry out desalination, concentrated, obtain.
embodiment 3,
The present embodiment relates to a kind of plant polypeptide protein extract, by following steps, obtains:
Step (1) is got roseleaf as plant material, first coarse crushing, then pulverize, obtain the pretreatment mixture of plant material;
Step (2) is got 4.0 weight portion pretreatment mixture and is soaked in 6.0 parts by weight of deionized water, then at 82 ℃ of lower seals, keeps 13 minutes, obtains and soaks mixed liquor;
It is 6.4 that step (3) is regulated the pH value that soaks mixed liquor, adds protease, and in protease and step (2), the mass ratio of pretreatment mixture is 0.8%, then cool under 42 ℃ the reaction 4.5h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is mixed clear liquid according to mass ratio with glacial acetic acid at 1: 1.2, with [MORBSA] [HSO
4] ionic liquid carries out acetylization reaction as catalyst, by acetylization reaction, the polypeptide protein end group in clear liquid carried out to shielding protection, catalyst amount account for clear liquid and glacial acetic acid gross mass 0.7%; after reaction finishes; separate described catalyst, then carry out desalination, concentrated, obtain.
embodiment 4,
The present embodiment relates to a kind of plant polypeptide protein extract, by following steps, obtains:
Step (1) is got Aloe as plant material, first coarse crushing, then pulverize, obtain the pretreatment mixture of plant material;
Step (2) is got 4.0 weight portion pretreatment mixture and is soaked in 6.0 parts by weight of deionized water, then at 83 ℃ of lower seals, keeps 12 minutes, obtains and soaks mixed liquor;
It is 6.6 that step (3) is regulated the pH value that soaks mixed liquor, adds protease, and in protease and step (2), the mass ratio of pretreatment mixture is 0.3%, then cool under 43 ℃ the reaction 4.5h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is mixed clear liquid according to mass ratio with glacial acetic acid at 1: 1.2, with [MORBSA] [HSO
4] ionic liquid carries out acetylization reaction as catalyst, by acetylization reaction, the polypeptide protein end group in clear liquid carried out to shielding protection, catalyst amount account for clear liquid and glacial acetic acid gross mass 0.8%; after reaction finishes; separate described catalyst, then carry out desalination, concentrated, obtain.
embodiment 5,
Step (1) is got the lavandula angustifolia petal as plant material, first coarse crushing, then pulverize, obtain the pretreatment mixture of plant material;
Step (2) is got 3.0 weight portion pretreatment mixture and is soaked in 7.0 parts by weight of deionized water, then at 80 ℃ of lower seals, keeps 15 minutes, obtains and soaks mixed liquor;
It is 6.2 that step (3) is regulated the pH value that soaks mixed liquor, adds protease, and in protease and step (2), the mass ratio of pretreatment mixture is 1.0%, then cool under 40 ℃ the reaction 5h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is reacted the polypeptide protein end group in clear liquid is carried out to shielding protection by mixed anhydride method again; mixed anhydride method is used the chloro-carbonic acid isobutyl to be reacted; in chloro-carbonic acid isobutyl and step (3), the mass ratio of clear liquid is 1: 1.3; then carry out desalination, concentrated, obtain.
embodiment 6,
Step (1) is got the Camellia sinensis fruit as plant material, first coarse crushing, then pulverize, obtain the pretreatment mixture of plant material;
Step (2) is got 5.0 weight portion pretreatment mixture and is soaked in 5.0 parts by weight of deionized water, then at 85 ℃ of lower seals, keeps 10 minutes, obtains and soaks mixed liquor;
It is 6.8 that step (3) is regulated the pH value that soaks mixed liquor, adds protease, and in protease and step (2), the mass ratio of pretreatment mixture is 0.1%, then cool under 45 ℃ the reaction 4h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is reacted the polypeptide protein end group in clear liquid is carried out to shielding protection by mixed anhydride method again; mixed anhydride method is used the chloro-carbonic acid isobutyl to be reacted; in chloro-carbonic acid isobutyl and step (3), the mass ratio of clear liquid is 1: 1.5; then carry out desalination, concentrated, obtain.
embodiment 7,
Step (1) is got the Sargassum leaf as plant material, first coarse crushing, then pulverize, obtain the pretreatment mixture of plant material;
Step (2) is got 4.0 weight portion pretreatment mixture and is soaked in 6.0 parts by weight of deionized water, then at 82 ℃ of lower seals, keeps 13 minutes, obtains and soaks mixed liquor;
It is 6.4 that step (3) is regulated the pH value that soaks mixed liquor, adds protease, and in protease and step (2), the mass ratio of pretreatment mixture is 0.8%, then cool under 42 ℃ the reaction 4.5h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is reacted the polypeptide protein end group in clear liquid is carried out to shielding protection by mixed anhydride method again; mixed anhydride method is used the chloro-carbonic acid isobutyl to be reacted; in chloro-carbonic acid isobutyl and step (3), the mass ratio of clear liquid is 1: 1.4; then carry out desalination, concentrated, obtain.
embodiment 8,
Step (1) is got Zante currant as plant material, first coarse crushing, then pulverize, obtain the pretreatment mixture of plant material;
Step (2) is got 4.0 weight portion pretreatment mixture and is soaked in 6.0 parts by weight of deionized water, then at 83 ℃ of lower seals, keeps 12 minutes, obtains and soaks mixed liquor;
It is 6.5 that step (3) is regulated the pH value that soaks mixed liquor, adds protease, and in protease and step (2), the mass ratio of pretreatment mixture is 0.5%, then cool under 43 ℃ the reaction 4.5h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is reacted the polypeptide protein end group in clear liquid is carried out to shielding protection by mixed anhydride method again; mixed anhydride method is used the chloro-carbonic acid isopropyl esters to be reacted; in chloro-carbonic acid isopropyl esters and step (3), the mass ratio of clear liquid is 1: 1.4; then carry out desalination, concentrated, obtain.
embodiment 9,
Step (1) is got the Flos Matricariae chamomillae petal as plant material, first coarse crushing, then pulverize, obtain the pretreatment mixture of plant material;
Step (2) is got 3.0 weight portion pretreatment mixture and is soaked in 7.0 parts by weight of deionized water, then at 80 ℃ of lower seals, keeps 15 minutes, obtains and soaks mixed liquor;
It is 6.5 that step (3) is regulated the pH value that soaks mixed liquor, adds protease, and in protease and step (2), the mass ratio of pretreatment mixture is 0.5%, then cool under 40 ℃ the reaction 5h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is first mixed clear liquid according to mass ratio with glacial acetic acid at 1: 1.3, with [MORBSA] [HSO
4] ionic liquid carries out acetylization reaction as catalyst the polypeptide protein end group in clear liquid is carried out to shielding protection; catalyst amount account for clear liquid and glacial acetic acid gross mass 0.6%; after reaction finishes; separating catalyst; then add the chloro-carbonic acid isopropyl esters, according to the mixed anhydride method reaction, the polypeptide protein end group in clear liquid is carried out to shielding protection; in chloro-carbonic acid isopropyl esters and step (3), the mass ratio of clear liquid is 1: 1.5, then carries out desalination, concentrated, obtains.
embodiment 10,
Step (1) is got the membranous bract of Herba Saussureae Involueratae as plant material, first coarse crushing, then pulverize, obtain the pretreatment mixture of plant material;
Step (2) is got 5.0 weight portion pretreatment mixture and is soaked in 5.0 parts by weight of deionized water, then at 85 ℃ of lower seals, keeps 10 minutes, obtains and soaks mixed liquor;
It is 6.8 that step (3) is regulated the pH value that soaks mixed liquor, adds protease, and in protease and step (2), the mass ratio of pretreatment mixture is 0.1%, then cool under 45 ℃ the reaction 4h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is first mixed clear liquid according to mass ratio with glacial acetic acid at 1: 1.1, with [MORBSA] [HSO
4] ionic liquid carries out acetylization reaction as catalyst the polypeptide protein end group in clear liquid is carried out to shielding protection; catalyst amount account for clear liquid and glacial acetic acid gross mass 0.9%; after reaction finishes; separate described catalyst; then add the chloro-carbonic acid isobutyl, according to the mixed anhydride method reaction, the polypeptide protein end group in clear liquid is carried out to shielding protection; in chloro-carbonic acid isobutyl and step (3), the mass ratio of clear liquid is 1: 1.3, then carries out desalination, concentrated, obtains.
the evaluation of implementation result
The polypeptide protein extract that above-described embodiment is manufactured; with specific plant fragrance; its main chemical constitution is the compound that contains peptide bond; the peptide linkage content is less than 10; molecular weight is little; good with the affinity of water, be easy to be penetrated into by skin the skin inside of human body, as biocatalyzer (enzyme), the transhipment that helps skin metabolism regulating action, immanoprotection action, material and storage, motion and supporting function, participate in the transmission of iuntercellular information.
Because raw material adopts natural plant, extract is had no side effect safely, and technique is simple, the original effect of plant does not have destroyed, kept extracting the curative effect of product, improved the stability of storage, production efficiency is high, be suitable for large-scale industrial production, can be widely used in the fields such as food, health care medicine, cosmetics.
Above specific embodiments of the invention are described.It will be appreciated that, the present invention is not limited to above-mentioned specific implementations, and those skilled in the art can make various distortion or modification within the scope of the claims, and this does not affect flesh and blood of the present invention.
Claims (10)
1. a plant polypeptide protein extract, is characterized in that, described extract obtains by following steps:
Step (1) is got the membranous bract of Herba Saussureae Involueratae, a kind of as plant material containing in the red Semen Coicis of rough bark, roseleaf, Aloe, lavandula angustifolia petal, Camellia sinensis fruit, Sargassum leaf, Zante currant, Flos Matricariae chamomillae petal, first coarse crushing, pulverize again, obtain the pretreatment mixture of plant material;
Step (2) is got the described pretreatment mixture of 3.0～5.0 weight portion and is soaked in 5.0～7.0 parts by weight of deionized water, then at 80～85 ℃ of lower seals, keeps 10～15 minutes, obtains and soaks mixed liquor;
The pH value that step (3) is regulated described immersion mixed liquor is 6.2～6.8, add protease, described in described protease and step (2), the mass ratio of pretreatment mixture is 0.1～1.0%, then cool under 40～45 ℃ the reaction 4～5h that is hydrolyzed, centrifugal, precipitation, filter, stratification, obtain clear liquid;
Step (4) is carried out shielding protection by acetylization reaction and/or mixed anhydride method reaction to the polypeptide protein end group in described clear liquid again, then carries out desalination, concentrated, obtains.
2. plant polypeptide protein extract according to claim 1, is characterized in that, in step (2), described pretreatment mixture is 4.0 weight portions, and described deionized water is 6.0 weight portions.
3. plant polypeptide protein extract according to claim 1, is characterized in that, in step (2), at 82～83 ℃ of lower seals, keeps 12～13 minutes, obtains described immersion mixed liquor.
4. plant polypeptide protein extract according to claim 1, is characterized in that, in step (3), described pH value is 6.4～6.6, and the mass ratio of described protease and described pretreatment mixture is 0.3～0.8%.
5. plant polypeptide protein extract according to claim 1, is characterized in that, in step (3), cools under 42～43 ℃ the reaction 4～5h that is hydrolyzed.
6. plant polypeptide protein extract according to claim 1, is characterized in that, described acetylization reaction comprises following concrete steps: by described clear liquid and glacial acetic acid according to mass ratio 1: (1.1～1.3) are mixed, with [MORBSA] [HSO
4] ionic liquid carries out acetylization reaction as catalyst, described catalyst amount account for described clear liquid and described glacial acetic acid gross mass 0.6～0.9%, reaction separates described catalyst after finishing.
7. plant polypeptide protein extract according to claim 6, is characterized in that, the mass ratio of described clear liquid and glacial acetic acid is 1: 1.2, described catalyst amount account for described clear liquid and described glacial acetic acid gross mass 0.7～0.8%.
8. plant polypeptide protein extract according to claim 1, it is characterized in that, described mixed anhydride method reaction is used chloro-carbonic acid isopropyl esters and/or chloro-carbonic acid isobutyl to be reacted, described in described chloro-carbonic acid isopropyl esters and step (3), the mass ratio of clear liquid is 1: (1.3～1.5), described in described chloro-carbonic acid isobutyl and step (3), the mass ratio of clear liquid is 1: (1.3～1.5).
9. plant polypeptide protein extract according to claim 8, it is characterized in that, described in described chloro-carbonic acid isopropyl esters and step (3), the mass ratio of clear liquid is 1: 1.4, and described in described chloro-carbonic acid isobutyl and step (3), the mass ratio of clear liquid is 1: 1.4.
10. the described plant polypeptide protein extract of claim 1 to 9 any one is as the application of cell active factor in cosmetics.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104530197A (en) * | 2015-01-22 | 2015-04-22 | 马淑娟 | Water retention peptide extracted from salix mongolica and application of water retention peptide in cosmetics |
| CN104719920A (en) * | 2015-04-09 | 2015-06-24 | 广州超亚化妆品科技有限公司 | Polypeptide amino acid mixture and its preparation method and applications |
| CN105287332A (en) * | 2015-10-19 | 2016-02-03 | 广州瑾洋化妆品有限公司 | Whitening and moisturizing composition containing floral essence polypeptide and preparation method and application of whitening and moisturizing composition |
| CN105624252A (en) * | 2016-03-30 | 2016-06-01 | 蔡庭守 | Saussurea involucrate micro-molecular peptide extract as well as preparation method and application thereof |
| CN105662925A (en) * | 2016-02-23 | 2016-06-15 | 孟繁好 | Cosmetic with freckle removing function |
| CN109453100A (en) * | 2018-12-29 | 2019-03-12 | 拉芳家化股份有限公司 | A kind of full ingredient natural origin and the lotion without deionized water |
| CN113249421A (en) * | 2021-04-27 | 2021-08-13 | 天津科技大学 | Golden-silk-royal chrysanthemum protein polypeptide and preparation and application thereof |
| CN113667708A (en) * | 2021-08-23 | 2021-11-19 | 山东林森生物制品股份有限公司 | Preparation method of plant intercellular substance |
-
2013
- 2013-09-02 CN CN2013103935872A patent/CN103446022A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| SKKO110: "《百度百科》", 18 June 2013, HTTP://BAIKE.BAIDU.COM/VIEW/2768704.HTM?FR=ALADDIN * |
| 周虹等主编: "《生物化学与分子生物学高级教程》", 31 January 2002, 科学出版社 * |
| 徐淑芹等: ""美发护发化妆品中的植物蛋白"", 《日用化学工业》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104530197A (en) * | 2015-01-22 | 2015-04-22 | 马淑娟 | Water retention peptide extracted from salix mongolica and application of water retention peptide in cosmetics |
| CN104719920A (en) * | 2015-04-09 | 2015-06-24 | 广州超亚化妆品科技有限公司 | Polypeptide amino acid mixture and its preparation method and applications |
| CN104719920B (en) * | 2015-04-09 | 2017-03-08 | 广州超亚化妆品科技有限公司 | Polypeptide amino acid mixture and preparation method and application |
| CN105287332A (en) * | 2015-10-19 | 2016-02-03 | 广州瑾洋化妆品有限公司 | Whitening and moisturizing composition containing floral essence polypeptide and preparation method and application of whitening and moisturizing composition |
| CN105287332B (en) * | 2015-10-19 | 2017-12-05 | 广州瑾洋化妆品有限公司 | A kind of skin whitening, moisturizing composition comprising flower extraction polypeptide, preparation method and application |
| CN105662925A (en) * | 2016-02-23 | 2016-06-15 | 孟繁好 | Cosmetic with freckle removing function |
| CN105662925B (en) * | 2016-02-23 | 2018-05-11 | 杭州悦萱堂化妆品有限公司 | A kind of cosmetics with spot-removing function |
| CN105624252A (en) * | 2016-03-30 | 2016-06-01 | 蔡庭守 | Saussurea involucrate micro-molecular peptide extract as well as preparation method and application thereof |
| CN109453100A (en) * | 2018-12-29 | 2019-03-12 | 拉芳家化股份有限公司 | A kind of full ingredient natural origin and the lotion without deionized water |
| CN113249421A (en) * | 2021-04-27 | 2021-08-13 | 天津科技大学 | Golden-silk-royal chrysanthemum protein polypeptide and preparation and application thereof |
| CN113249421B (en) * | 2021-04-27 | 2023-03-03 | 天津科技大学 | Golden-silk-royal chrysanthemum protein polypeptide and preparation and application thereof |
| CN113667708A (en) * | 2021-08-23 | 2021-11-19 | 山东林森生物制品股份有限公司 | Preparation method of plant intercellular substance |
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Application publication date: 20131218 |