CN109172452B - Compound extract of white paeony root and sophora flower and application of compound extract as cosmetic additive - Google Patents
Compound extract of white paeony root and sophora flower and application of compound extract as cosmetic additive Download PDFInfo
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- CN109172452B CN109172452B CN201811238769.1A CN201811238769A CN109172452B CN 109172452 B CN109172452 B CN 109172452B CN 201811238769 A CN201811238769 A CN 201811238769A CN 109172452 B CN109172452 B CN 109172452B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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Abstract
The invention relates to a compound extract of white paeony root and sophora flower and application thereof as a cosmetic additive, wherein the preparation method of the compound extract of white paeony root and sophora flower comprises the following steps: (1) taking 4-5 parts of white paeony root and 1 part of sophora flower by weight, crushing and uniformly mixing, sieving by a 80-mesh sieve, and adding ionic liquid [ BMIM ]]PF6Heating the phosphate buffer solution (pH 5.0) and cellulase to 40-50 ℃, soaking and extracting for 3-5 hours, filtering, removing filter residues, standing and layering the filtrate, and collecting an ionic liquid layer; (2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2-3 times, combining ethyl acetate phases, washing with water and saturated sodium chloride in sequence, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain the compound extract of white paeony root and sophora flower.
Description
Technical Field
The invention belongs to the field of plant extracts and daily chemicals, and particularly relates to a compound extract of white paeony root and sophora flower and application thereof as a cosmetic additive.
Background
Radix paeoniae alba (Paeonia lactiflora Pall.) is a root of a plant peony which is boiled with water, peeled and dried in the sun, is a traditional Chinese medicinal material, is bitter in nature and slightly cold, and has the effects of calming the liver, relieving pain, nourishing blood, regulating menstruation, astringing yin and arresting sweating. The main chemical components of the composition are volatile oil, monoterpene, triterpenes, flavonoids and the like. Flos Sophorae Immaturus is the dried flower and bud of Leguminosae plant flos Sophorae Immaturus (Sophora japonica L.), and is collected and dried in summer to remove branches, stalks and impurities when flower is opened or bud is formed, wherein the former is called flos Sophorae Immaturus, and the latter is called flos Sophorae Immaturus. The chemical components mainly comprise flavone, saponin, fatty acid and the like. The invention provides a method for extracting effective components of white paeony root and sophora flower by utilizing hydrophobic ionic liquid to assist enzymolysis, and obtains a compound extract of the white paeony root and the sophora flower, wherein the extract has excellent performances in the aspects of free radical scavenging, tyrosinase inhibition, SOD enzyme activity and the like, and is expected to be developed and used as an additive of whitening and sunscreen cosmetics.
Disclosure of Invention
The invention provides a compound extract of white paeony root and sophora flower, which is characterized in that the preparation method of the compound extract of white paeony root and sophora flower comprises the following steps:
(1) taking 4-5 parts of white paeony root and 1 part of sophora flower by weight, crushing and uniformly mixing, sieving by a 80-mesh sieve, and adding ionic liquid [ BMIM ]]PF6Heating the phosphate buffer solution (pH 5.0) and cellulase to 40-50 ℃, soaking and extracting for 3-5 hours, filtering, removing filter residues, standing and layering the filtrate, and collecting an ionic liquid layer;
(2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2-3 times, combining ethyl acetate phases, washing with water and saturated sodium chloride in sequence, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain the compound extract of white paeony root and sophora flower.
In the step (1), the dosage of the cellulase is 3-5% of the mass of the sophora japonica, and the enzyme activity is 10-40 u/mg; the ionic liquid [ BMIM]PF6The dosage of the phosphate buffer solution is 10-15 times of the total mass of radix Paeoniae alba and flos Sophorae Immaturus, wherein the ionic liquid [ BMIM]PF6The mass fraction of (A) is 15-20%.
Another embodiment of the invention provides a preparation method of a compound extract of white paeony root and sophora flower, which is characterized by comprising the following steps:
(1) taking 4-5 parts of white paeony root and 1 part of sophora flower by weight, crushing and uniformly mixing, sieving by a 80-mesh sieve, and adding ionic liquid [ BMIM ]]PF6Heating the phosphate buffer solution (pH 5.0) and cellulase to 40-50 ℃, soaking and extracting for 3-5 hours, filtering, removing filter residues, standing and layering the filtrate, and collecting an ionic liquid layer;
(2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2-3 times, combining ethyl acetate phases, washing with water and saturated sodium chloride in sequence, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain the compound extract of white paeony root and sophora flower.
In the step (1), the dosage of the cellulase is 3-5% of the mass of the sophora japonica, and the enzyme activity is 10-40 u/mg; the ionic liquid [ BMIM]PF6The dosage of the phosphate buffer solution is 10-15 times of the total mass of radix Paeoniae alba and flos Sophorae Immaturus, wherein the ionic liquid [ BMIM]PF6The mass fraction of (A) is 15-20%.
The other embodiment of the invention provides application of the compound extract of white paeony root and sophora flower as an antioxidant.
Another embodiment of the invention provides application of the compound extract of white paeony root and sophora flower in the aspect of scavenging free radicals. The radical is preferably DPPH radical or superoxide anion radical.
The other embodiment of the invention provides application of the compound extract of white paeony root and sophora japonica in inhibiting tyrosinase.
Another embodiment of the invention provides an application of the compound extract of white paeony root and sophora flower in preparing a cosmetic additive for preventing ultraviolet injury.
The other embodiment of the invention provides application of the compound extract of white paeony root and sophora flower as a cosmetic additive. The cosmetic is preferably whitening agent, sunscreen agent, etc.
The other embodiment of the invention provides application of the compound extract of white paeony root and sophora flower in preparing cosmetics.
The other embodiment of the invention provides application of the compound extract of white paeony root and sophora flower in preparing SOD cosmetics. The SOD cosmetics are selected from SOD facial mask, SOD honey, SOD snake powder, etc.
Ionic liquids [ BMIM ] according to the invention]PF6The Chinese name of (A) is 1-butyl-3-methylimidazolium hexafluorophosphate; the phosphate buffer solution (pH 5.0) can be prepared from two or more of sodium dihydrogen phosphate (or potassium), disodium hydrogen phosphate (or potassium), sodium hydroxide and phosphoric acid; for example, the solution can be prepared by adjusting 0.2mol/L sodium dihydrogen phosphate solution to pH5.0 with sodium hydroxide solution.
Compared with the prior art, the invention has the advantages that: (1) the present invention utilizes ionic liquids ([ BMIM)]PF6) Extracting effective components of radix Paeoniae alba and flos Sophorae Immaturus with enzyme-binding method, and hydrophobic ionic liquid [ BMIM ]]PF6The enzyme is used as a biological catalysis medium and an extraction solvent, can effectively stabilize the transition state of enzyme-substrate, reduce the activation energy of reaction and enable the enzyme to show higher catalytic activity; (2) the invention determines the optimal mixing ratio of the white paeony root and the sophora flower, and the activity of the extract is higher than that of the extract obtained by extracting the white paeony root or the sophora flower alone or other mixing ratios (such as 1:1), which is probably caused by the concomitant extraction of plants; (3) radix paeoniae alba and sophora japonica prepared by the inventionThe flower compound extract shows strong activity in the aspects of free radical removal and tyrosinase inhibition; (4) the compound extract of white paeony root and sophora flower prepared by the invention shows strong SOD enzyme activity.
Detailed Description
In order to facilitate a further understanding of the invention, the following examples are provided to illustrate it in more detail. However, these examples are only for better understanding of the present invention and are not intended to limit the scope or the principle of the present invention, and the embodiments of the present invention are not limited to the following.
Example 1
(1) Taking 400g of white paeony root and 100g of sophora flower, crushing and uniformly mixing the white paeony root and the sophora flower, sieving the mixture by a 80-mesh sieve, and adding ionic liquid [ BMIM ]]PF6Phosphate buffer solution (pH5.0, 5.0kg, containing 1.0kg of [ BMIM ]]PF6) Heating to 40 deg.C, soaking and extracting for 5 hr, filtering, removing residue, standing for layering, and collecting ionic liquid layer;
(2) and (2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2 times, combining ethyl acetate phases, washing with water and saturated sodium chloride in sequence, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain the compound extract (8.62g, hereinafter referred to as product A) of the white paeony root and the sophora flower.
Example 2
(1) Mixing radix Paeoniae alba 500g and flos Sophorae Immaturus 100g, pulverizing, sieving with 80 mesh sieve, and adding ionic liquid [ BMIM ]]PF6Phosphate buffer solution (pH5.0, 9.0kg, containing 1.35kg of [ BMIM ]]PF6) Heating to 50 deg.C, soaking and extracting for 3 hr, filtering, removing residue, standing for layering, and collecting ionic liquid layer;
(2) and (2) extracting the ionic liquid layer obtained in the step (1) for 3 times by using ethyl acetate with the same volume, combining ethyl acetate phases, sequentially washing by using water and saturated sodium chloride, drying and filtering an organic layer by using anhydrous sodium sulfate, concentrating and drying filtrate to obtain the compound extract (9.42g, hereinafter referred to as a product B) of the white paeony root and the sophora flower.
Example 3
(1) Pulverizing radix Paeoniae alba 100g and flos Sophorae Immaturus 100g, mixing, sieving with 80 mesh sieve, and adding ionic liquid [ BMIM ]]PF6Phosphate buffer solution (pH5.0, 5.0kg, containing 1.0kg of [ BMIM ]]PF6) Heating to 40 deg.C, soaking and extracting for 5 hr, filtering, removing residue, standing for layering, and collecting ionic liquid layer;
(2) and (2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2 times, combining ethyl acetate phases, washing with water and saturated sodium chloride in sequence, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain a compound extract (3.79g, hereinafter referred to as a product C) of the white paeony root and the sophora flower.
Example 4
(1) Pulverizing radix Paeoniae alba 400g, mixing, sieving with 80 mesh sieve, adding ionic liquid [ BMIM ]]PF6Phosphate buffer solution (pH5.0, 5.0kg, containing 1.0kg of [ BMIM ]]PF6) Heating to 40 deg.C, soaking and extracting for 5 hr, filtering, removing residue, standing for layering, and collecting ionic liquid layer;
(2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2 times, combining ethyl acetate phases, washing with water and saturated sodium chloride in sequence, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain radix Paeoniae alba extract (5.02g, hereinafter referred to as product D).
Example 5
(1) Taking 100g of sophora flower, crushing and uniformly mixing the sophora flower, sieving the sophora flower with a 80-mesh sieve, and adding the BMIM-containing ionic liquid]PF6Phosphate buffer solution (pH5.0, 5.0kg, containing 1.0kg of [ BMIM ]]PF6) Heating to 40 deg.C, soaking and extracting for 5 hr, filtering, removing residue, standing for layering, and collecting ionic liquid layer;
(2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2 times, combining ethyl acetate phases, washing with water and saturated sodium chloride in sequence, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain flos Sophorae Immaturus extract (1.54g, hereinafter referred to as product E).
Example 6
Taking 400g of white paeony root and 100g of sophora flower, crushing and uniformly mixing the white paeony root and the sophora flower, sieving by a sieve of 80 meshes, adding phosphate buffer solution (pH5.0, 5.0kg) and cellulase (3g,40u/mg), heating to 40 ℃, soaking and extracting for 5 hours, filtering, removing filter residues, extracting the filtrate for 2 times by using ethyl acetate with the same volume, combining ethyl acetate phases, washing by using water and saturated sodium chloride in sequence, drying an organic layer by using anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain a compound extract (5.96g, hereinafter referred to as a product F) of the white paeony root and the sophora flower.
Example 7
Taking 400G of white paeony root and 100G of sophora flower, crushing and uniformly mixing the white paeony root and the sophora flower, sieving by a sieve of 80 meshes, adding 5.0kg of water, heating to 40 ℃, soaking and extracting for 5 hours, filtering, removing filter residues, concentrating the filtrate, and drying to obtain a compound extract (3.88G, hereinafter referred to as a product G) of the white paeony root and the sophora flower.
Example 8
An appropriate amount of the products A-G and the products D + E (mass ratio 1:1) were dissolved in DMSO to prepare a solution with a mass concentration of 1.0mg/mL, and the DPPH radical scavenging ability, superoxide anion scavenging ability, and tyrosinase inhibition rate of the products A-G and the products D + E (mass ratio 1:1) were tested according to the previously described method (Chinese patent application No. CN201810216275.7), and the results are shown in Table 1.
TABLE 1
Example 9 superoxide dismutase (SOD for short) Activity test
According to the Chinese patent application number: the SOD enzyme activities of the products A to G were measured by the "riboflavin-NBT method" described in CN201711352387.7, and the results showed that only IC of product A, B was observed503.0-3.5 μ g/mL, IC of other products50Are all more than 20 mug/mL.
Claims (5)
1. The compound extract of white paeony root and sophora flower is characterized in that the preparation method of the compound extract of white paeony root and sophora flower comprises the following steps:
(1) taking 4-5 parts of white paeony root by weight1 part of sophora flower, pulverizing, uniformly mixing, sieving with a 80-mesh sieve, adding ionic liquid [ BMIM ]]PF6Heating the phosphate buffer solution and the cellulase to 40-50 ℃, soaking and extracting for 3-5 hours, filtering, removing filter residues, standing and layering the filtrate, and collecting an ionic liquid layer;
(2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2-3 times, combining ethyl acetate phases, washing with water and saturated sodium chloride in sequence, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain the compound extract of white paeony root and sophora flower;
in the step (1), the dosage of the cellulase is 3-5% of the mass of the sophora japonica, and the enzyme activity is 10-40 u/mg; the ion-containing liquid [ BMIM]PF6The pH of the phosphate buffer solution is 5.0, and the dosage of the phosphate buffer solution is 10-15 times of the sum of the mass of the white paeony root and the sophora flower, wherein the ionic liquid [ BMIM ]]PF6The mass fraction of (A) is 15-20%.
2. The use of the compound extract of radix paeoniae alba and flos sophorae as claimed in claim 1 in the preparation of cosmetics for inhibiting tyrosinase.
3. Use of the compound extract of paeonia lactiflora pall and sophora japonica as claimed in claim 1 in preparing a cosmetic additive for preventing ultraviolet injury.
4. Use of the compound extract of paeonia lactiflora pall and sophora japonica as claimed in claim 1 as a cosmetic additive.
5. Use according to any one of claims 3 to 4, characterized in that the cosmetic additives are selected from whitening agents, sunscreens.
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| CN111388367B (en) * | 2020-03-25 | 2021-09-24 | 江南大学 | Composition for inhibiting melanin at multiple target points, preparation method and application of composition in cosmetics |
| CN112545943B (en) * | 2020-12-20 | 2022-04-08 | 时垠(上海)生物科技有限公司 | SOD cosmetic additive containing plant extract |
| CN114469795A (en) * | 2020-12-20 | 2022-05-13 | 刘爽 | Preparation method of SOD cosmetic additive extract |
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| WO2015039619A1 (en) * | 2013-09-22 | 2015-03-26 | 天士力制药集团股份有限公司 | Use of yangxueqingnao formulation in preparation of medicine for treating alzheimer's disease |
| CN105055289A (en) * | 2015-07-20 | 2015-11-18 | 毛春红 | Multi-effect skin caring and smoothening traditional Chinese medicine lotion and preparation method thereof |
| CN107212215A (en) * | 2017-05-11 | 2017-09-29 | 徐州工程学院 | A kind of ginkgo sophora flower composite beverage and preparation method thereof |
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| DE102007002410A1 (en) * | 2007-01-17 | 2008-07-24 | Merck Patent Gmbh | Synthesis of chroman derivatives |
| CN101708210B (en) * | 2009-12-04 | 2011-10-05 | 广东工业大学 | Application of pagodatree flower general flavone extract in preparing medicament for inhabiting cutaneous pigmentation |
| NL2004835C2 (en) * | 2010-06-07 | 2011-12-08 | Univ Leiden | Process for extracting materials from biological material. |
| CN103524320A (en) * | 2013-09-11 | 2014-01-22 | 西安交通大学 | Substituted benzophenone and preparation method thereof |
| CN108078885B (en) * | 2018-02-05 | 2020-09-18 | 海南师范大学 | Dendrobium extract and application thereof in preparation of whitening sunscreen agent |
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| WO2015039619A1 (en) * | 2013-09-22 | 2015-03-26 | 天士力制药集团股份有限公司 | Use of yangxueqingnao formulation in preparation of medicine for treating alzheimer's disease |
| CN105055289A (en) * | 2015-07-20 | 2015-11-18 | 毛春红 | Multi-effect skin caring and smoothening traditional Chinese medicine lotion and preparation method thereof |
| CN107212215A (en) * | 2017-05-11 | 2017-09-29 | 徐州工程学院 | A kind of ginkgo sophora flower composite beverage and preparation method thereof |
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Denomination of invention: The invention relates to a compound extract of white peony and Sophora japonica flower and its application as a cosmetic additive Effective date of registration: 20210809 Granted publication date: 20200929 Pledgee: Industrial and Commercial Bank of China Limited Guangzhou Yunshan sub branch Pledgor: Guangdong fanmilin Biotechnology Co., Ltd Registration number: Y2021440000253 |
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