CN108977483A - A kind of technique that papain prepares lotus leaf polypeptide - Google Patents
A kind of technique that papain prepares lotus leaf polypeptide Download PDFInfo
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- CN108977483A CN108977483A CN201810412056.6A CN201810412056A CN108977483A CN 108977483 A CN108977483 A CN 108977483A CN 201810412056 A CN201810412056 A CN 201810412056A CN 108977483 A CN108977483 A CN 108977483A
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- lotus leaf
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention discloses the techniques that a kind of papain prepares lotus leaf polypeptide, first dry lotus leaf green grass in an oven, and dry powder is made in crushing；10-12 times of weight of lotus leaf dry powder of water is added in lotus leaf dry powder later, obtains lotus leaf slurry；Adjust lotus leaf slurries pH value be 6.5-7.5, be added papain digested, using enzyme deactivation, vacuum freeze drying and etc. lotus leaf polypeptide powder is made.Lotus leaf has rich in protein, rich in amino acid necessary to human body, is the mankind one of the food plant good for health with health care, is the quality raw materials of polypeptide preparation.Present invention process prepares lotus leaf polypeptide by enzymatic hydrolysis, may be used as food additives, natural, and is easy to be absorbed by the body, good for health without side-effects.Change to the Land use systems of lotus leaf increases the utilization ways of lotus leaf, increases plantation profit.
Description
Technical field
The invention discloses the techniques that a kind of papain prepares lotus leaf polypeptide, belong to technical field of polypeptide.
Technical background
Contain a large amount of protein in lotus leaf, rich in amino acid necessary to human body, and there is no animality cholesterol.Simultaneously
Contain the functional components such as a variety of alkaloids such as nuciferine, miltanthin and Nuciferine and vitamin C, polysaccharide.Have clearing heat and detoxicating, cool
Blood, hemostasis effect, be the food plant one of beneficial to human health and health care, be polypeptide preparation quality raw materials.Lotus leaf
Interspersing for landscape is had been used as, part harvesting is used as food materials, causes the significant wastage of resource, seriously reduce the benefit of lotus leaf
With value, present invention process carries out enzymatic hydrolysis by extraction lotus leaf albumen and prepares lotus leaf polypeptide, may be used as food additives, naturally
Antioxidant, and be easy to be absorbed by the body, it is good for health without side-effects.Change to the Land use systems of lotus leaf, increases lotus
The utilization ways of leaf increase plantation profit.
Summary of the invention
The technical problem to be solved by the present invention is in view of the deficiencies of the prior art, provide a kind of preparation side of lotus leaf polypeptide
Method.
The technical scheme is that
(1) prepare lotus leaf, lotus leaf is cleaned；
(2) lotus leaf is placed in 105 DEG C of baking ovens the 1h that finishes, is subsequently placed in 60h in 70 DEG C of baking ovens until drying, until weight
It does not change, it is spare to crushed 60 meshes；
(3) 10-12 times of weight of lotus leaf dry powder of water is added in the lotus leaf dry powder that will be obtained by step 2, by lotus leaf dry powder and
Water stirs evenly, and obtains lotus leaf slurry；
(4) pH value for adjusting lotus leaf slurries is 6.5-7.5, and papain is added and is digested, digests at 50-55 DEG C
After 3.5-4h, it is placed in enzyme deactivation in 10 minutes in 90-100 DEG C of water-bath, taking supernatant is lotus leaf protein hydrolyzate；
(5) obtained hydrolyzate is centrifugated, removes lower sediment, extract supernatant；
(6) supernatant is subjected to vacuum freeze drying into powder to get lotus leaf polypeptide products.
In embodiments of the present invention, in step (1), it is anti-can to inactivate the intracorporal protease of plant by 105 DEG C of water-removing 1h
Only breaks down proteins.
In embodiments of the present invention, in step (3), lotus leaf dry powder and water are stirred 0.5-1 hours.
In embodiments of the present invention, in step (4), enzyme content is 1000- in the papain solution of addition
1500U/g。
In embodiments of the present invention, in step (4), the temperature of enzyme deactivation is 90-100 DEG C, and high temperature can preferably go out
Living protein enzyme.
Specific embodiment:
Technology contents of the invention are described further below with reference to embodiment, following embodiments be it is illustrative, no
It is restrictive, cannot be limited the scope of protection of the present invention with following embodiments.
Embodiment 1: a kind of papain prepares the technique of lotus leaf polypeptide, processes in accordance with the following steps:
(1) prepare lotus leaf, lotus leaf is cleaned；
(2) lotus leaf is placed in 105 DEG C of baking ovens the 1h that finishes, is subsequently placed in 60h in 70 DEG C of baking ovens until drying, until weight
It does not change, it is spare to crushed 60 meshes；
(3) the lotus leaf dry powder obtained by step 2 is added to the water of 10 times of lotus leaf dry powder weight, lotus leaf dry powder is stirred with water
It mixes uniformly, obtains lotus leaf slurry；
(4) pH value for adjusting lotus leaf slurries is 6.5, and papain (1000U/g) is added by lotus leaf dry powder weight and carries out
Enzymatic hydrolysis, after 50 DEG C of enzymatic hydrolysis 3.5h, is placed in enzyme deactivation in 10 minutes in 90 DEG C of water-bath, and taking supernatant is lotus leaf protein hydrolyzate；
(5) obtained hydrolyzate is centrifugated, removes lower sediment, extract supernatant；
(6) supernatant is subjected to vacuum freeze drying into powder to get lotus leaf polypeptide products.
Embodiment 2: a kind of papain prepares the technique of lotus leaf polypeptide, processes in accordance with the following steps:
(1) prepare lotus leaf, lotus leaf is cleaned；
(2) lotus leaf is placed in 105 DEG C of baking ovens the 1h that finishes, is subsequently placed in 60h in 70 DEG C of baking ovens until drying, until weight
It does not change, it is spare to crushed 60 meshes；
(3) the lotus leaf dry powder obtained by step 2 is added to the water of 12 times of lotus leaf dry powder weight, lotus leaf dry powder is stirred with water
It mixes uniformly, obtains lotus leaf slurry；
(4) pH value for adjusting lotus leaf slurries is 7.5, and papain (1500U/g) is added by lotus leaf dry powder weight and carries out
Enzymatic hydrolysis, after 55 DEG C of enzymatic hydrolysis 4h, is placed in enzyme deactivation in 10 minutes in 100 DEG C of water-bath, and taking supernatant is lotus leaf protein hydrolyzate；
(5) obtained hydrolyzate is centrifugated, removes lower sediment, extract supernatant；
(6) supernatant is subjected to vacuum freeze drying into powder to get lotus leaf polypeptide products.
Claims (5)
1. the technique that a kind of papain prepares lotus leaf polypeptide, which is characterized in that specific preparation process is as follows:
(1) prepare lotus leaf, lotus leaf is cleaned；
(2) lotus leaf is placed in 105 DEG C of baking ovens the 1h that finishes, is subsequently placed in 60h in 70 DEG C of baking ovens until drying, until weight is not sent out
It is raw to change, it is spare to crushed 60 meshes；
(3) the lotus leaf dry powder obtained by step 2 is added to 10-12 times of weight of lotus leaf dry powder of water, lotus leaf dry powder is stirred with water
It mixes uniformly, obtains lotus leaf slurry；
(4) pH value for adjusting lotus leaf slurries is 6.5-7.5, and papain is added and is digested, in 50-55 DEG C of enzymatic hydrolysis 3.5-4h
Afterwards, it is placed in enzyme deactivation in 10 minutes in 90-100 DEG C of water-bath, taking supernatant is lotus leaf protein hydrolyzate；
(5) obtained hydrolyzate is centrifugated, removes lower sediment, extract supernatant；
(6) supernatant is subjected to vacuum freeze drying into powder to get lotus leaf polypeptide products.
2. the technique that a kind of papain according to claim 1 prepares lotus leaf polypeptide, characterized in that the step
(1) in, 105 DEG C of water-removing 1h, can inactivate the intracorporal protease of plant prevents breaks down proteins.
3. the technique that a kind of papain according to claim 1 prepares lotus leaf polypeptide, characterized in that the step
(3) in, lotus leaf dry powder and water are stirred 0.5-1 hours.
4. the technique that a kind of papain according to claim 1 prepares lotus leaf polypeptide, characterized in that the step
(4) in, enzyme content is 1000-1500U/g in the papain solution of addition.
5. the technique that a kind of papain according to claim 1 prepares lotus leaf polypeptide, characterized in that the step
(4) in, the temperature of enzyme deactivation is 90-100 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810412056.6A CN108977483A (en) | 2018-04-24 | 2018-04-24 | A kind of technique that papain prepares lotus leaf polypeptide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810412056.6A CN108977483A (en) | 2018-04-24 | 2018-04-24 | A kind of technique that papain prepares lotus leaf polypeptide |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497913A (en) * | 2009-03-02 | 2009-08-05 | 湘潭大学 | Preparation of lotus seed protein polypeptide |
| CN105624252A (en) * | 2016-03-30 | 2016-06-01 | 蔡庭守 | Saussurea involucrate micro-molecular peptide extract as well as preparation method and application thereof |
| WO2017200340A2 (en) * | 2016-05-20 | 2017-11-23 | 주식회사 미싹바이오 | Low-molecular collagen peptide and method for preparing food composition by using same |
-
2018
- 2018-04-24 CN CN201810412056.6A patent/CN108977483A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497913A (en) * | 2009-03-02 | 2009-08-05 | 湘潭大学 | Preparation of lotus seed protein polypeptide |
| CN105624252A (en) * | 2016-03-30 | 2016-06-01 | 蔡庭守 | Saussurea involucrate micro-molecular peptide extract as well as preparation method and application thereof |
| WO2017200340A2 (en) * | 2016-05-20 | 2017-11-23 | 주식회사 미싹바이오 | Low-molecular collagen peptide and method for preparing food composition by using same |
Non-Patent Citations (2)
| Title |
|---|
| 刘贝涛: "荷叶的化学成分和药理作用探究", 《当代化工研究》 * |
| 李恒等: "木瓜蛋白酶制备广昌白莲降血压活性肽的研究", 《食品工业科技》 * |
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Application publication date: 20181211 |
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