CN108546726A - A kind of lotus leaf polypeptide preparation process - Google Patents
A kind of lotus leaf polypeptide preparation process Download PDFInfo
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- CN108546726A CN108546726A CN201810285563.8A CN201810285563A CN108546726A CN 108546726 A CN108546726 A CN 108546726A CN 201810285563 A CN201810285563 A CN 201810285563A CN 108546726 A CN108546726 A CN 108546726A
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- lotus leaf
- dry powder
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- General Health & Medical Sciences (AREA)
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a kind of lotus leaf polypeptide preparation processes, first dry lotus leaf green grass in an oven, and dry powder is made in crushing；The water of 10 12 times of lotus leaf dry powder weight is added in lotus leaf dry powder later, obtains lotus leaf slurry；Adjust lotus leaf slurries pH value be 8.5 9, be added neutral proteinase digested, using enzyme deactivation, vacuum freeze drying and etc. lotus leaf polypeptide powder is made.Lotus leaf has rich in protein, is the mankind one of the food plant good for health with health care rich in amino acid necessary to human body, is quality raw materials prepared by polypeptide.Present invention process prepares lotus leaf polypeptide by enzymolysis, may be used as food additives, natural, and is easy to be absorbed by the body, good for health without side-effects.Change to the Land use systems of lotus leaf increases the utilization ways of lotus leaf, increases plantation profit.
Description
Technical field
The invention discloses a kind of lotus leaf polypeptide preparation processes, belong to technical field of polypeptide.
Technical background
Lotus leaf has rich in protein, rich in the amino acid necessary to human body, and is free of animality cholesterol.Together
A variety of alkaloids such as Shi Hanyou nuciferines, miltanthin and Nuciferine and vitamin C, polysaccharide.There are clearing heat and detoxicating, cool blood, hemostasis
Effect is the mankind one of the food plant good for health with health care, is quality raw materials prepared by polypeptide.Lotus leaf has been used as wind
Scape is interspersed, and part harvesting is used as food materials, causes the significant wastage of resource, seriously reduces the utility value of lotus leaf, this hair
Bright technique carries out enzymolysis and prepares lotus leaf polypeptide by extracting lotus leaf albumen, may be used as food additives, natural, and
And be easy to be absorbed by the body, it is good for health without side-effects.Change to the Land use systems of lotus leaf, increase lotus leaf utilizes way
Diameter increases plantation profit.
Invention content
The technical problem to be solved by the present invention is in view of the deficiencies of the prior art, provide a kind of preparation side of lotus leaf polypeptide
Method.
The technical scheme is that：
(1) prepare lotus leaf, lotus leaf is cleaned；
(2) lotus leaf is placed in 105 DEG C of baking ovens the 1h that finishes, is subsequently placed in 60h in 70 DEG C of baking ovens until drying, until weight
It does not change, it is spare to crushed 60 mesh sieve；
(3) water of 10-12 times of lotus leaf dry powder weight is added in the lotus leaf dry powder that will be obtained by step 2, by lotus leaf dry powder and
Water stirs evenly, and obtains lotus leaf slurry；
(4) pH value for adjusting lotus leaf slurries is 8.5-9, and neutral proteinase is added and is digested, 3.5- is digested at 50-55 DEG C
After 4h, it is placed in enzyme deactivation in 10 minutes in 90-100 DEG C of water-bath, it is lotus leaf protein hydrolyzate to take supernatant；
(5) obtained hydrolyzate is centrifuged, removes lower sediment, extract supernatant；
(6) supernatant is subjected to vacuum freeze drying into powder to get lotus leaf polypeptide products.
In embodiments of the present invention, in step (1), 105 DEG C of water-removing 1h, the protease that can be inactivated in plant is prevented
Only breaks down proteins.
In embodiments of the present invention, in step (3), lotus leaf dry powder stirs 0.5-1 hours with water.
In embodiments of the present invention, in step (4), enzyme content is 3000- in the neutral protein enzyme solutions of addition
3500U/g。
In embodiments of the present invention, in step (4), the temperature of enzyme deactivation is 90-100 DEG C, and high temperature can preferably go out
Living protein enzyme.
Specific implementation mode：
With reference to embodiment to the present invention technology contents be described further, following embodiments be it is illustrative, no
It is restrictive, protection scope of the present invention cannot be limited with following embodiments.
Embodiment 1：A kind of lotus leaf polypeptide production methods, are processed in accordance with the following steps：
(1) prepare lotus leaf, lotus leaf is cleaned；
(2) lotus leaf is placed in 105 DEG C of baking ovens the 1h that finishes, is subsequently placed in 60h in 70 DEG C of baking ovens until drying, until weight
It does not change, it is spare to crushed 60 mesh sieve；
(3) the lotus leaf dry powder obtained by step 2 is added to the water of 10 times of lotus leaf dry powder weight, lotus leaf dry powder is stirred with water
It mixes uniformly, obtains lotus leaf slurry；
(4) pH value for adjusting lotus leaf slurries is 8.5, and neutral proteinase (3000U/g), which is added, by lotus leaf dry powder weight carries out
Enzymolysis is placed in enzyme deactivation in 10 minutes in 90 DEG C of water-bath after digesting 3.5h at 50 DEG C, and it is lotus leaf protein hydrolyzate to take supernatant；
(5) obtained hydrolyzate is centrifuged, removes lower sediment, extract supernatant；
(6) supernatant is subjected to vacuum freeze drying into powder to get lotus leaf polypeptide products.
Embodiment 2：A kind of lotus leaf polypeptide production methods, are processed in accordance with the following steps：
(1) prepare lotus leaf, lotus leaf is cleaned；
(2) lotus leaf is placed in 105 DEG C of baking ovens the 1h that finishes, is subsequently placed in 60h in 70 DEG C of baking ovens until drying, until weight
It does not change, it is spare to crushed 60 mesh sieve；
(3) the lotus leaf dry powder obtained by step 2 is added to the water of 12 times of lotus leaf dry powder weight, lotus leaf dry powder is stirred with water
It mixes uniformly, obtains lotus leaf slurry；
(4) pH value for adjusting lotus leaf slurries is 9, and neutral proteinase (3500U/g), which is added, by lotus leaf dry powder weight carries out enzyme
Solution is placed in enzyme deactivation in 10 minutes in 100 DEG C of water-bath after digesting 4h at 55 DEG C, and it is lotus leaf protein hydrolyzate to take supernatant；
(5) obtained hydrolyzate is centrifuged, removes lower sediment, extract supernatant；
(6) supernatant is subjected to vacuum freeze drying into powder to get lotus leaf polypeptide products.
Claims (5)
1. a kind of lotus leaf polypeptide preparation process, which is characterized in that specific preparation process is as follows：
(1) prepare lotus leaf, lotus leaf is cleaned；
(2) lotus leaf is placed in 105 DEG C of baking ovens the 1h that finishes, is subsequently placed in 60h in 70 DEG C of baking ovens until drying, until weight is not sent out
It is raw to change, it is spare to crushed 60 mesh sieve；
(3) the lotus leaf dry powder obtained by step 2 is added to the water of 10-12 times of lotus leaf dry powder weight, lotus leaf dry powder is stirred with water
It mixes uniformly, obtains lotus leaf slurry；
(4) pH value for adjusting lotus leaf slurries is 8.5-9, and neutral proteinase is added and is digested, 3.5-4h is digested at 50-55 DEG C
Afterwards, it is placed in enzyme deactivation in 10 minutes in 90-100 DEG C of water-bath, it is lotus leaf protein hydrolyzate to take supernatant；
(5) obtained hydrolyzate is centrifuged, removes lower sediment, extract supernatant；
(6) supernatant is subjected to vacuum freeze drying into powder to get lotus leaf polypeptide products.
2. a kind of lotus leaf polypeptide preparation process according to claim 1, characterized in that in the step (1), 105 DEG C
Finish 1h, and the protease that can be inactivated in plant prevents breaks down proteins.
3. a kind of lotus leaf polypeptide preparation process according to claim 1, characterized in that in the step (3), lotus leaf is dry
Powder stirs 0.5-1 hours with water.
4. a kind of lotus leaf polypeptide preparation process according to claim 1, characterized in that in the step (4), addition
Enzyme content is 3000-3500U/g in neutral protein enzyme solutions.
5. a kind of lotus leaf polypeptide preparation process according to claim 1, characterized in that in the step (4), enzyme deactivation
Temperature is 90-100 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810285563.8A CN108546726A (en) | 2018-03-22 | 2018-03-22 | A kind of lotus leaf polypeptide preparation process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810285563.8A CN108546726A (en) | 2018-03-22 | 2018-03-22 | A kind of lotus leaf polypeptide preparation process |
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| CN201810285563.8A Pending CN108546726A (en) | 2018-03-22 | 2018-03-22 | A kind of lotus leaf polypeptide preparation process |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497913A (en) * | 2009-03-02 | 2009-08-05 | 湘潭大学 | Preparation of lotus seed protein polypeptide |
| CN105624252A (en) * | 2016-03-30 | 2016-06-01 | 蔡庭守 | Saussurea involucrate micro-molecular peptide extract as well as preparation method and application thereof |
| WO2017200340A2 (en) * | 2016-05-20 | 2017-11-23 | 주식회사 미싹바이오 | Low-molecular collagen peptide and method for preparing food composition by using same |
-
2018
- 2018-03-22 CN CN201810285563.8A patent/CN108546726A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497913A (en) * | 2009-03-02 | 2009-08-05 | 湘潭大学 | Preparation of lotus seed protein polypeptide |
| CN105624252A (en) * | 2016-03-30 | 2016-06-01 | 蔡庭守 | Saussurea involucrate micro-molecular peptide extract as well as preparation method and application thereof |
| WO2017200340A2 (en) * | 2016-05-20 | 2017-11-23 | 주식회사 미싹바이오 | Low-molecular collagen peptide and method for preparing food composition by using same |
Non-Patent Citations (2)
| Title |
|---|
| 刘贝涛: "荷叶的化学成分和药理作用探究", 《当代化工研究》 * |
| 庄玮婧等: "碱性蛋白酶酶解莲子蛋白制备抗氧化肽的研究", 《中国食品科学技术学会第十一届年会论文摘要集》 * |
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Application publication date: 20180918 |
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