CN105063141A - Small-molecule Cordyceps militaris active peptide and preparing method thereof - Google Patents
Small-molecule Cordyceps militaris active peptide and preparing method thereof Download PDFInfo
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Abstract
The invention relates to a small-molecule Cordyceps militaris active peptide and a preparing method thereof. The preparing method includes the steps of drying, crushing, extracting, enzymolysis, filtering and drying. Enzymolysis includes first enzymolysis, second enzymolysis and third enzymolysis. The first enzymolysis process includes adding beta-glucanase accounting for 0.5% to 1% of dry weight of extracted Cordyceps militaris into Cordyceps militaris extract acquired by extracting Cordyceps militaris, adjusting solution pH to 4 to 5.5, setting temperature of first enzymolysis to 40 DEG C to 60 DEG C, and allowing first enzymolysis to be carried out for 4h to 6h. hard cell walls of Cordyceps militaris are broken down by a beta-glucanase enzymolysis technique, dissolution of cellular contents is benefited, a smooth extracting process is facilitated, and effective components such as proteins, Cordyceps polysaccharide and Cordyceps adenosine can be extracted more efficiently; Cordyceps militaris can be enzymolyzed by the multiple enzymolysis method, and the proteins in Cordyceps militaris can be fully enzymolyzed.
Description
Technical field
The present invention relates to biologically active substance and extract field, be specifically related to a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide and preparation method thereof.
Background technology
Cordyceps militaris (L.) Link. has another name called Cordyceps militaris (L.) Link., Cordyceps militaris etc., a kind of famous and precious Chinese medicine and high tonic, its activeconstituents is extremely similar to famous medicinal fungi Cordyceps sinensis with function, containing chinese caterpillar fungus polypeptide, Chinese caterpillar fungus adenosine, cordycepic acid, the biologically active substances such as Cordyceps polysaccharide and multiple amino acids, VITAMIN, trace element.Cordyceps militaris (L.) Link. is consistent with the key effects composition of wild cordyceps, but on content and on some special functional component, Cordyceps militaris (L.) Link. is 10 times of Cordyceps sinensis to tens times.Cordycepin in Cordyceps militaris (L.) Link. is 35 times of Cordyceps sinensis, and the VITAMIN B4 in Cordyceps militaris (L.) Link. is 40 times of Cordyceps sinensis, and the Nucleotide total content in Cordyceps militaris (L.) Link. is 6.25 times of wild cordyceps.
Cordyceps militaris (L.) Link. have antitumor, improve body's immunity, anti-oxidant, anti-ageing, protection liver kidney and the aspect different physiological roles such as cardiovascular.The PRODUCTION TRAITS of the cordycepin of Cordyceps militaris (L.) Link., Cordyceps polysaccharide, cordycepic acid aspect is more, but less for the PRODUCTION TRAITS of protein active peptide aspect.Containing protein 26%, sugar 28%, fat 2.9% in Cordyceps militaris (L.) Link., protein content is abundanter.
Containing rich in protein and amino acid in Cordyceps militaris (L.) Link., by enzymolysis protein matter, can produce multiple Polypetide Nitrogen and the amino acid with physiologically active, these peptides can directly absorbed by the bodyly utilize, and then regulate physical mechanism more rapidly and effectively, promote health.Among the people, Cordyceps militaris (L.) Link. is many to take by decocting the mode such as to stew, edible and carry inconvenience.Utilize the related products that Cordyceps militaris (L.) Link. produces in the market, great majority are Cordyceps militaris (L.) Link. and pulverize fine grinding, although particle is less, but wherein still containing the material such as a large amount of Mierocrystalline cellulose and macro-molecular protein, do not reach the effect directly absorbed, simultaneously because the embedding of the materials such as Mierocrystalline cellulose, also have impact on absorbing of functional component.Also there is not the report being prepared Cordyceps militaris (L.) Link. Gly-His-Lys by the method for enzymolysis Cordyccps-militaris-(L.)-link. Sporophore in the market.
Summary of the invention
Technical problem to be solved by this invention is the defect for prior art, provides a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide and preparation method thereof.
The technical scheme that the present invention solves the problems of the technologies described above is as follows: a kind of preparation method of small molecules Cordyceps militaris (L.) Link. bioactive peptide, comprises the following steps: drying and crushing, extraction, enzymolysis, filtration and drying successively;
Described enzymolysis comprises primary enzymolysis, secondary enzymolysis and three enzymolysis, obtains small molecules Cordyceps militaris (L.) Link. bioactive peptide solution through three enzymolysis; Described primary enzymolysis process is: add through extracting in the Cordyceps militaris (L.) Link. extracting solution obtained the beta-glucanase being equivalent to extract Cordyceps militaris (L.) Link. dry weight 0.5%-1% used to Cordyceps militaris (L.) Link., regulator solution pH is 4-5.5, primary enzymolysis temperature is 40 DEG C-60 DEG C, and the primary enzymolysis time is 4h-6h.
The invention has the beneficial effects as follows: the present invention utilizes beta-glucan enzyme resolving tech to abolish the hard cell walls of Cordyceps militaris (L.) Link., be conducive to the stripping of cell Dissolve things inside, be conducive to carrying out smoothly of leaching process, improve the extraction efficiency of the functional components such as protein, Cordyceps polysaccharide and Chinese caterpillar fungus adenosine; The present invention utilizes repeatedly enzyme solution to carry out enzymolysis to Cordyceps militaris (L.) Link., can carry out abundant enzymolysis to the protein in Cordyceps militaris (L.) Link..
On the basis of technique scheme, the present invention can also do following improvement.
Further, described secondary enzymolysis process is: lower the temperature after the enzymolysis solution after primary enzymolysis is boiled 1min-5min centrifugal, after centrifugal removal of impurities, add papoid and carry out secondary enzymolysis in primary enzymolysis liquid, the add-on of papoid is equivalent to Cordyceps militaris (L.) Link. dry weight 0.5%-1%; The pH regulating enzymolysis solution is 6-7.5, and secondary enzymolysis temperature is 45 DEG C-55 DEG C, and the secondary enzymolysis time is 2h-4h.
Further, described three enzymolysis process are: will add the trypsinase being equivalent to Cordyceps militaris (L.) Link. dry weight 1%-1.5% in the enzymolysis solution after secondary enzymolysis, the pH value regulating enzymolysis solution is 7.5-8.5, and three times hydrolysis temperature is 45 DEG C-55 DEG C, and three enzymolysis times are 4h-6h.
Further, described filtration procedure is: be down to room temperature after the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution obtained through enzymolysis is boiled 1min-5min, to the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution after the enzyme that goes out be boiled through filter press, remove the macromolecular components in solution and insoluble composition.
Further, described drying and crushing process is: Cordyceps militaris (L.) Link. is dry and be crushed to 40 order-60 orders.
Further, described leaching process is: add in extractor by the Cordyceps militaris (L.) Link. powder through drying and crushing, add distilled water and stir in extractor, and the solid-to-liquid ratio in extractor is 1:8-10.
The beneficial effect of above-mentioned further scheme is adopted to be: to adopt low temperature water extraction to be combined with enzymolysis, to avoid in traditional technology high temperature extraction to the destruction of active substance in Cordyceps militaris (L.) Link., ensure that the biological activity of the functional components such as adenosine, substantially reduce extraction time simultaneously, improve production efficiency.
Further, described drying process adopts spraying dry.
Adopt aforesaid method i.e. obtained a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide of the present invention, described small molecules Cordyceps militaris (L.) Link. bioactive peptide is that molecular-weight average is less than 1000 daltonian oligopeptides.
Embodiment
Be described principle of the present invention and feature with the following Examples, example, only for explaining the present invention, is not intended to limit scope of the present invention.
Embodiment 1
The preparation method of a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide of the present embodiment, specifically comprises the following steps:
1) drying and crushing: Cordyceps militaris (L.) Link. is dry and be crushed to 40 orders.
2) extract: add in extractor by the Cordyceps militaris (L.) Link. powder through drying and crushing, add the distilled water of 20 DEG C and stir in extractor, the solid-to-liquid ratio in extractor is 1:8.The present embodiment adopts low temperature water extraction to be combined with enzymolysis, avoids high temperature extraction in traditional technology and, to the destruction of active substance in Cordyceps militaris (L.) Link., ensure that the biological activity of the functional components such as adenosine, substantially reduce extraction time simultaneously, improve production efficiency.
3) enzymolysis: described enzymolysis comprises primary enzymolysis, secondary enzymolysis and three enzymolysis, obtains small molecules Cordyceps militaris (L.) Link. bioactive peptide solution through three enzymolysis; Described primary enzymolysis process is: in Cordyceps militaris (L.) Link. extracting solution, add the beta-glucanase being equivalent to extract Cordyceps militaris (L.) Link. dry weight 0.5% used, regulator solution pH is 4, and primary enzymolysis temperature is 40 DEG C, and the primary enzymolysis time is 4h.
Secondary enzymolysis process is: lower the temperature after the enzymolysis solution after primary enzymolysis is boiled 1min centrifugal, and after centrifugal removal of impurities, add papoid and carry out secondary enzymolysis in primary enzymolysis liquid, the add-on of papoid is equivalent to Cordyceps militaris (L.) Link. dry weight 0.5%; The pH regulating enzymolysis solution is 6, and secondary enzymolysis temperature is 45 DEG C, and the secondary enzymolysis time is 2h.
Three times enzymolysis process is: will add the trypsinase being equivalent to Cordyceps militaris (L.) Link. dry weight 1% in the enzymolysis solution after secondary enzymolysis, and the pH value of adjustment enzymolysis solution is 7.5, three hydrolysis temperatures is 45 DEG C, and three enzymolysis times are 4h.
The present embodiment utilizes beta-glucan enzyme resolving tech to abolish the hard cell walls of Cordyceps militaris (L.) Link., is conducive to the stripping of entocyte, is conducive to carrying out smoothly of leaching process, improves the extraction efficiency of the functional components such as protein, Cordyceps polysaccharide and Chinese caterpillar fungus adenosine; The present embodiment utilizes repeatedly enzyme solution to carry out enzymolysis to Cordyceps militaris (L.) Link., can carry out abundant enzymolysis to the protein in Cordyceps militaris (L.) Link..
4) filter: be down to room temperature after the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution obtained through enzymolysis is boiled 1min, will the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution after the enzyme that goes out be boiled through filter press, remove the macromolecular components in solution and insoluble composition.
5) dry: described drying process adopts spraying dry, namely obtains small molecules Cordyceps militaris (L.) Link. Gly-His-Lys finished product of the present invention after drying terminates.
Embodiment 2
The preparation method of a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide of the present embodiment, specifically comprises the following steps:
1) drying and crushing: Cordyceps militaris (L.) Link. is dry and be crushed to 50 orders.
2) extract: add in extractor by the Cordyceps militaris (L.) Link. powder through drying and crushing, add the distilled water of 22 DEG C and stir in extractor, the solid-to-liquid ratio in extractor is 1:9.The present embodiment adopts low temperature water extraction to be combined with enzymolysis, avoids high temperature extraction in traditional technology and, to the destruction of active substance in Cordyceps militaris (L.) Link., ensure that the biological activity of the functional components such as adenosine, substantially reduce extraction time simultaneously, improve production efficiency.
3) enzymolysis: described enzymolysis comprises primary enzymolysis, secondary enzymolysis and three enzymolysis, obtains small molecules Cordyceps militaris (L.) Link. bioactive peptide solution through three enzymolysis; Described primary enzymolysis process is: in Cordyceps militaris (L.) Link. extracting solution, add the beta-glucanase being equivalent to extract Cordyceps militaris (L.) Link. dry weight 0.8% used, regulator solution pH is 5, and primary enzymolysis temperature is 50 DEG C, and the primary enzymolysis time is 5h.
Secondary enzymolysis process is: lower the temperature after the enzymolysis solution after primary enzymolysis is boiled 3min centrifugal, and after centrifugal removal of impurities, add papoid and carry out secondary enzymolysis in primary enzymolysis liquid, the add-on of papoid is equivalent to Cordyceps militaris (L.) Link. dry weight 0.8%; The pH regulating enzymolysis solution is 7, and secondary enzymolysis temperature is 50 DEG C, and the secondary enzymolysis time is 3h.
Three times enzymolysis process is: will add the trypsinase being equivalent to Cordyceps militaris (L.) Link. dry weight 1.5% in the enzymolysis solution after secondary enzymolysis, and the pH value of adjustment enzymolysis solution is 8, three hydrolysis temperatures is 50 DEG C, and three enzymolysis times are 5h.
The present embodiment utilizes beta-glucan enzyme resolving tech to abolish the hard cell walls of Cordyceps militaris (L.) Link., is conducive to the stripping of entocyte, is conducive to carrying out smoothly of leaching process, improves the extraction efficiency of the functional components such as protein, Cordyceps polysaccharide and Chinese caterpillar fungus adenosine; The present embodiment utilizes repeatedly enzyme solution to carry out enzymolysis to Cordyceps militaris (L.) Link., can carry out abundant enzymolysis to the protein in Cordyceps militaris (L.) Link..
4) filter: be down to room temperature after the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution obtained through enzymolysis is boiled 3min, will the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution after the enzyme that goes out be boiled through filter press, remove the macromolecular components in solution and insoluble composition.
5) dry: described drying process adopts spraying dry, namely obtains small molecules Cordyceps militaris (L.) Link. Gly-His-Lys finished product of the present invention after drying terminates.
Embodiment 3
The preparation method of a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide of the present embodiment, specifically comprises the following steps:
1) drying and crushing: Cordyceps militaris (L.) Link. is dry and be crushed to 60 orders.
2) extract: add in extractor by the Cordyceps militaris (L.) Link. powder through drying and crushing, add the distilled water of 25 DEG C and stir in extractor, the solid-to-liquid ratio in extractor is 1:10.The present embodiment adopts low temperature water extraction to be combined with enzymolysis, avoids high temperature extraction in traditional technology and, to the destruction of active substance in Cordyceps militaris (L.) Link., ensure that the biological activity of the functional components such as adenosine, substantially reduce extraction time simultaneously, improve production efficiency.
3) enzymolysis: described enzymolysis comprises primary enzymolysis, secondary enzymolysis and three enzymolysis, obtains small molecules Cordyceps militaris (L.) Link. bioactive peptide solution through three enzymolysis; Described primary enzymolysis process is: in Cordyceps militaris (L.) Link. extracting solution, add the beta-glucanase being equivalent to extract Cordyceps militaris (L.) Link. dry weight 1% used, regulator solution pH is 5.5, and primary enzymolysis temperature is 60 DEG C, and the primary enzymolysis time is 6h.
Secondary enzymolysis process is: lower the temperature after the enzymolysis solution after primary enzymolysis is boiled 5min centrifugal, and after centrifugal removal of impurities, add papoid and carry out secondary enzymolysis in primary enzymolysis liquid, the add-on of papoid is equivalent to Cordyceps militaris (L.) Link. dry weight 1%; The pH regulating enzymolysis solution is 7.5, and secondary enzymolysis temperature is 55 DEG C, and the secondary enzymolysis time is 4h.
Three times enzymolysis process is: will add the trypsinase being equivalent to Cordyceps militaris (L.) Link. dry weight 1.5% in the enzymolysis solution after secondary enzymolysis, and the pH value of adjustment enzymolysis solution is 8.5, three hydrolysis temperatures is 55 DEG C, and three enzymolysis times are 6h.
The present embodiment utilizes beta-glucan enzyme resolving tech to abolish the hard cell walls of Cordyceps militaris (L.) Link., is conducive to the stripping of entocyte, is conducive to carrying out smoothly of leaching process, improves the extraction efficiency of the functional components such as protein, Cordyceps polysaccharide and Chinese caterpillar fungus adenosine; The present embodiment utilizes repeatedly enzyme solution to carry out enzymolysis to Cordyceps militaris (L.) Link., can carry out abundant enzymolysis to the protein in Cordyceps militaris (L.) Link..
4) filter: be down to room temperature after the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution obtained through enzymolysis is boiled 5min, will the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution after the enzyme that goes out be boiled through filter press, remove the macromolecular components in solution and insoluble composition.
5) dry: described drying process adopts spraying dry, namely obtains small molecules Cordyceps militaris (L.) Link. Gly-His-Lys finished product of the present invention after drying terminates.
Embodiment 4
The preparation method of a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide of the present embodiment, specifically comprises the following steps:
1) drying and crushing: Cordyceps militaris (L.) Link. is dry and be crushed to 55 orders.
2) extract: add in extractor by the Cordyceps militaris (L.) Link. powder through drying and crushing, add the distilled water of 20 DEG C and stir in extractor, the solid-to-liquid ratio in extractor is 1:8.The present embodiment adopts low temperature water extraction to be combined with enzymolysis, avoids high temperature extraction in traditional technology and, to the destruction of active substance in Cordyceps militaris (L.) Link., ensure that the biological activity of the functional components such as adenosine, substantially reduce extraction time simultaneously, improve production efficiency.
3) enzymolysis: described enzymolysis comprises primary enzymolysis, secondary enzymolysis and three enzymolysis, obtains small molecules Cordyceps militaris (L.) Link. bioactive peptide solution through three enzymolysis; Described primary enzymolysis process is: in Cordyceps militaris (L.) Link. extracting solution, add the beta-glucanase being equivalent to extract Cordyceps militaris (L.) Link. dry weight 1% used, regulator solution pH is 5.5, and primary enzymolysis temperature is 60 DEG C, and the primary enzymolysis time is 6h.
Secondary enzymolysis process is: lower the temperature after the enzymolysis solution after primary enzymolysis is boiled 1min centrifugal, and after centrifugal removal of impurities, add papoid and carry out secondary enzymolysis in primary enzymolysis liquid, the add-on of papoid is equivalent to Cordyceps militaris (L.) Link. dry weight 0.5%; The pH regulating enzymolysis solution is 6, and secondary enzymolysis temperature is 55 DEG C, and the secondary enzymolysis time is 2h.
Three times enzymolysis process is: will add the trypsinase being equivalent to Cordyceps militaris (L.) Link. dry weight 1% in the enzymolysis solution after secondary enzymolysis, and the pH value of adjustment enzymolysis solution is 7.5, three hydrolysis temperatures is 45 DEG C, and three enzymolysis times are 4h.
The present embodiment utilizes beta-glucan enzyme resolving tech to abolish the hard cell walls of Cordyceps militaris (L.) Link., is conducive to the stripping of entocyte, is conducive to carrying out smoothly of leaching process, improves the extraction efficiency of the functional components such as protein, Cordyceps polysaccharide and Chinese caterpillar fungus adenosine; The present embodiment utilizes repeatedly enzyme solution to carry out enzymolysis to Cordyceps militaris (L.) Link., can carry out abundant enzymolysis to the protein in Cordyceps militaris (L.) Link..
4) filter: be down to room temperature after the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution obtained through enzymolysis is boiled 1min, will the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution after the enzyme that goes out be boiled through filter press, remove the macromolecular components in solution and insoluble composition.
5) dry: described drying process adopts spraying dry, namely obtains small molecules Cordyceps militaris (L.) Link. Gly-His-Lys finished product of the present invention after drying terminates.
Embodiment 5
The preparation method of a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide of the present embodiment, specifically comprises the following steps:
1) drying and crushing: Cordyceps militaris (L.) Link. is dry and be crushed to 60 orders.
2) extract: add in extractor by the Cordyceps militaris (L.) Link. powder through drying and crushing, add the distilled water of 25 DEG C and stir in extractor, the solid-to-liquid ratio in extractor is 1:10.The present embodiment adopts low temperature water extraction to be combined with enzymolysis, avoids high temperature extraction in traditional technology and, to the destruction of active substance in Cordyceps militaris (L.) Link., ensure that the biological activity of the functional components such as adenosine, substantially reduce extraction time simultaneously, improve production efficiency.
3) enzymolysis: described enzymolysis comprises primary enzymolysis, secondary enzymolysis and three enzymolysis, obtains small molecules Cordyceps militaris (L.) Link. bioactive peptide solution through three enzymolysis; Described primary enzymolysis process is: in Cordyceps militaris (L.) Link. extracting solution, add the beta-glucanase being equivalent to extract Cordyceps militaris (L.) Link. dry weight 1% used, regulator solution pH is 5.5, and primary enzymolysis temperature is 60 DEG C, and the primary enzymolysis time is 6h.
Secondary enzymolysis process is: lower the temperature after the enzymolysis solution after primary enzymolysis is boiled 5min centrifugal, and after centrifugal removal of impurities, add papoid and carry out secondary enzymolysis in primary enzymolysis liquid, the add-on of papoid is equivalent to Cordyceps militaris (L.) Link. dry weight 0.5%-1%; The pH regulating enzymolysis solution is 7.5, and secondary enzymolysis temperature is 55 DEG C, and the secondary enzymolysis time is 4h.
Three times enzymolysis process is: will add the trypsinase being equivalent to Cordyceps militaris (L.) Link. dry weight 1.5% in the enzymolysis solution after secondary enzymolysis, and the pH value of adjustment enzymolysis solution is 8.5, three hydrolysis temperatures is 55 DEG C, and three enzymolysis times are 6h.
The present embodiment utilizes beta-glucan enzyme resolving tech to abolish the hard cell walls of Cordyceps militaris (L.) Link., is conducive to the stripping of entocyte, is conducive to carrying out smoothly of leaching process, improves the extraction efficiency of the functional components such as protein, Cordyceps polysaccharide and Chinese caterpillar fungus adenosine; The present embodiment utilizes repeatedly enzyme solution to carry out enzymolysis to Cordyceps militaris (L.) Link., can carry out abundant enzymolysis to the protein in Cordyceps militaris (L.) Link..
4) filter: be down to room temperature after the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution obtained through enzymolysis is boiled 3min, will the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution after the enzyme that goes out be boiled through filter press, remove the macromolecular components in solution and insoluble composition.
5) dry: described drying process adopts spraying dry, namely obtains small molecules Cordyceps militaris (L.) Link. Gly-His-Lys finished product of the present invention after drying terminates.
Adopt the molecular weight distribution of GB/T22729-2008 method to the small molecules sea cucumber Gly-His-Lys that the present embodiment 2 obtains to detect, detected result is as shown in table 1.
The molecular weight distribution table of table 1 small molecules sea cucumber Gly-His-Lys
| Molecular weight ranges | Peak area percent (%, λ 220nm) | Number-average molecular weight | Weight-average molecular weight |
| >5000 | 0.24 | 7708 | 8701 |
| 5000～3000 | 0.53 | 3622 | 3701 |
| 3000～2000 | 1.53 | 2327 | 2356 |
| 2000～1000 | 9.87 | 1263 | 1311 |
| 1000～500 | 34.82 | 755 | 770 |
| 500～180 | 48.57 | 295 | 321 |
| ＜180 | 4.44 | / | / |
As shown in Table 1, the molecular weight of the small molecules Cordyceps militaris (L.) Link. active peptide powder that method obtains described in embodiment 2 is adopted mainly to be distributed between 180 dalton-1000 dalton, small molecules Cordyceps militaris (L.) Link. active peptide powder good water solubility between this molecular weight area, is more conducive to absorption and the utilization of Active Components in Various Cordyceps militaris Strains.
Adopt GB5009.5-2010 to detect the peptide content of a kind of small molecules Cordyceps militaris (L.) Link. active peptide powder that method described in the present embodiment 2 obtains, acid-soluble protein content, total nitrogen content and weight loss on drying, detected result is as shown in table 2.
The peptide content of table 2 small molecules Cordyceps militaris (L.) Link. active peptide powder, acid-soluble protein content, total nitrogen content and weight loss on drying detected result
| Peptide content (g/100g) | 81.05 |
| Acid-soluble protein content (g/100g) | 78.10 |
| Total nitrogen content (g/100g) | 14.05 |
| Weight loss on drying (%) | 3.28 |
As shown in Table 2, the yield adopting method described in the present embodiment 2 to produce small molecules Cordyceps militaris (L.) Link. bioactive peptide is 80%-90%, wherein acid-soluble protein content is in the great majority, and is conducive to digesting and assimilating of human stomach, have antitumor, improve body immunity, the different physiological roles such as anti-oxidant.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. a preparation method for small molecules Cordyceps militaris (L.) Link. bioactive peptide, is characterized in that, comprises the following steps successively: drying and crushing, extraction, enzymolysis, filtration and drying;
Described enzymolysis comprises primary enzymolysis, secondary enzymolysis and three enzymolysis, obtains small molecules Cordyceps militaris (L.) Link. bioactive peptide solution through three enzymolysis; Described primary enzymolysis process is: add through extracting in the Cordyceps militaris (L.) Link. extracting solution obtained the beta-glucanase being equivalent to extract Cordyceps militaris (L.) Link. dry weight 0.5%-1% used to Cordyceps militaris (L.) Link., regulator solution pH is 4-5.5, primary enzymolysis temperature is 40 DEG C-60 DEG C, and the primary enzymolysis time is 4h-6h.
2. the preparation method of a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide according to claim 1, it is characterized in that, described secondary enzymolysis process is: lower the temperature after the enzymolysis solution after primary enzymolysis is boiled 1min-5min centrifugal, after centrifugal removal of impurities, in primary enzymolysis liquid, add papoid carry out secondary enzymolysis, the add-on of papoid is equivalent to the 0.5%-1% of Cordyceps militaris (L.) Link. dry weight; The pH regulating enzymolysis solution is 6-7.5, and secondary enzymolysis temperature is 45 DEG C-55 DEG C, and the secondary enzymolysis time is 2h-4h.
3. a kind of preparation method of small molecules Cordyceps militaris (L.) Link. bioactive peptide according to claim 1 or 2, it is characterized in that, described three enzymolysis process are: will add the trypsinase being equivalent to Cordyceps militaris (L.) Link. dry weight 1%-1.5% in the enzymolysis solution after secondary enzymolysis, the pH value regulating enzymolysis solution is 7.5-8.5, three times hydrolysis temperature is 45 DEG C-55 DEG C, and three enzymolysis times are 4h-6h.
4. the preparation method of a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide according to claim 1, it is characterized in that, described filtration procedure is: be down to room temperature after the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution obtained through enzymolysis is boiled 1min-5min, to the small molecules Cordyceps militaris (L.) Link. bioactive peptide solution after the enzyme that goes out be boiled through filter press, remove the macromolecular components in solution and insoluble composition.
5. the preparation method of a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide according to claim 1, it is characterized in that, described drying and crushing process is: Cordyceps militaris (L.) Link. is dry and be crushed to 40 order-60 orders.
6. the preparation method of a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide according to claim 1, it is characterized in that, described leaching process is: add in extractor by the Cordyceps militaris (L.) Link. powder through drying and crushing, add the distilled water of room temperature and stir in extractor, and the solid-to-liquid ratio in extractor is 1:8-10.
7. the preparation method of a kind of small molecules Cordyceps militaris (L.) Link. bioactive peptide according to claim 1, is characterized in that, described drying process adopts spraying dry.
8. a small molecules Cordyceps militaris (L.) Link. bioactive peptide, it is characterized in that, adopt method i.e. obtained small molecules Cordyceps militaris (L.) Link. bioactive peptide of the present invention as described in any one of claim 1-7, described small molecules Cordyceps militaris (L.) Link. bioactive peptide is that molecular-weight average is less than 1000 daltonian oligopeptides.
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| CN106434810A (en) * | 2016-11-18 | 2017-02-22 | 辽宁省农业科学院 | Preparation method of cordyceps militaris polypeptide dried powder |
| CN112813124A (en) * | 2021-01-05 | 2021-05-18 | 钱康南 | Small molecule active grass enzyme peptide, preparation method and application thereof |
| CN112890194A (en) * | 2021-02-05 | 2021-06-04 | 山西省农业科学院食用菌研究所 | Tongdong day lily nutrition essence syrup and preparation process thereof |
| CN115181775A (en) * | 2022-07-19 | 2022-10-14 | 张建明 | Extraction method of small molecular liver peptide |
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| CN106434810A (en) * | 2016-11-18 | 2017-02-22 | 辽宁省农业科学院 | Preparation method of cordyceps militaris polypeptide dried powder |
| CN112813124A (en) * | 2021-01-05 | 2021-05-18 | 钱康南 | Small molecule active grass enzyme peptide, preparation method and application thereof |
| CN112813124B (en) * | 2021-01-05 | 2024-05-07 | 钱康南 | Small-molecule active grass enzyme peptide and preparation method and application thereof |
| CN112890194A (en) * | 2021-02-05 | 2021-06-04 | 山西省农业科学院食用菌研究所 | Tongdong day lily nutrition essence syrup and preparation process thereof |
| CN115181775A (en) * | 2022-07-19 | 2022-10-14 | 张建明 | Extraction method of small molecular liver peptide |
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