CN103864481A - method for preparing microbial inoculum and in-situ decomposing method and structure by heaping whole corn stalks - Google Patents

method for preparing microbial inoculum and in-situ decomposing method and structure by heaping whole corn stalks Download PDF

Info

Publication number
CN103864481A
CN103864481A CN201310711004.6A CN201310711004A CN103864481A CN 103864481 A CN103864481 A CN 103864481A CN 201310711004 A CN201310711004 A CN 201310711004A CN 103864481 A CN103864481 A CN 103864481A
Authority
CN
China
Prior art keywords
maize straw
corn
microbial inoculum
thoroughly decomposed
urea
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201310711004.6A
Other languages
Chinese (zh)
Other versions
CN103864481B (en
Inventor
石俊雄
骆建军
仲统伟
黄建国
袁玲
张恒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
QINGDAO TIANDIYUAN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
Southwest University
Guizhou Institute of Tobacco Science
Original Assignee
QINGDAO TIANDIYUAN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
Southwest University
Guizhou Institute of Tobacco Science
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by QINGDAO TIANDIYUAN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd, Southwest University, Guizhou Institute of Tobacco Science filed Critical QINGDAO TIANDIYUAN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
Priority to CN201310711004.6A priority Critical patent/CN103864481B/en
Publication of CN103864481A publication Critical patent/CN103864481A/en
Application granted granted Critical
Publication of CN103864481B publication Critical patent/CN103864481B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Fertilizers (AREA)

Abstract

The invention discloses a method for preparing a microbial inoculum and an in-situ decomposing method and structure by heaping whole corn stalks. The high-temperature spore fiber degrading bacteria is taken as a corn stalk composting strain. The method comprises the steps of: heaping whole plant corn stalks with corn removed in a flat area in a corn planting filed in a horizontal and vertical alternating manner, wherein the total height of corn stalk heap is 150cm-180cm; pressing and compacting corn stalks tightly and spreading uniformly the stain and urea on the surface of the corn stalks during the heating process when the thickness of a corn stalk heat reaches up to 25cm-35cm, wherein the use amount of the stain and urea account for 0.1%-0.3% and 1.5% and 3% of weight of the corn stalks, respectively and the weight of each corn stalk heap is not less than 1000kg; covering the stacked corn stalks with agricultural membrane, then compacting the top of each corn stalk heap tightly with stone and forming holes in the agricultural membrane at the position under the stone so as to supply water through rainwater; and composting for 90 to 120 days. By the method, the mineralization and humification of organic matters can be promoted, decomposition of corn stalks is accelerated and compost quality is improved.

Description

Become thoroughly decomposed method and structure are heaped in the whole strain of bacterial preparation process and maize straw original position
Technical field
The present invention relates to the whole strain of a kind of bacterial preparation process and maize straw original position and heap become thoroughly decomposed method and structure, belong to fertilizer and preparing technical field thereof.
Background technology
?southwest is one of major production areas of China's corn, and nearly 6,202 ten thousand mu of maize sown areas, exceed 70% of upland grain sown area and output, produce every year 49096.8 ten thousand tons of maize straws.Because high slope, local mountain is steep, to plough mainly take arable land, slope as main, it exists plot little, and the problem that the gradient is large causes its both also field of unsuitable mechanize crushing maize stalk.China Patent No. is 2012100795739, patent name is for disclosing a kind of method of utilizing maize straw to make fertilizer in the patent of " a kind of maize straw is without microbial inoculum fertilizer and preparation method thereof ", but its preparation process for " a, maize straw is processed into 45-50mm length; B, stalk is soaked in water or water is drenched, makes in stalk Relative water content at 58-62%; C, adopt paving one deck stalk to water clear liquid dung in selected collected manure region, the Recycle design of spreading again one deck oil cake piles up moulding, after accumulation, obturage with mud; Every layer of stalk thickness is 30-40cm, and oil cake thickness is 0.4-0.6cm; D, pile up turn up overturning in 25 days once, then composting is obturaged again, and repetitive operation 3 times is to fermentation ends; Make fertilizer." though the method can make maize straw be utilized effectively, " carrying ", " pulverizing " and " turning " that it relates to maize straw, cause whole production process to need highly intensive labour, relate to mechanised equipment and the energy; Be subject to the restriction of above-mentioned condition, though make that the method is good to be difficult to popularize.Therefore, the general indwelling of maize straw is rotted or on-site incineration naturally in field, not only affects second stubble crop plantation, and wastes a large amount of organic resources, and cause serious atmospheric pollution.
The main component of maize straw is Mierocrystalline cellulose, hemicellulose and xylogen, and a small amount of nitrogen, phosphorus, potassium, calcium, magnesium, silicon etc.In western developed country and northern China, prepartion of land is vast, and maize straw is through also field of mechanical disintegration.After they enter soil, the energy and the nutritive substance that need are provided to microorganism, promote its growth and breeding.Meanwhile, microorganism is decomposed organic, supplies with plant nutrition; Partial organic substances is again under the effect of microorganism, and synthetic soil ulmin, improves soil physico-chemical property.Therefore, straw-returning has the function that fertilizer is improved the soil concurrently, is the mode of utilizing generally adopting both at home and abroad.But due to the restriction of landform and social factor, maize straw mechanical disintegration also field is not suitable for Southwestern China mountain area and similar area.Therefore be badly in need of maize straw that a kind of applicable southwest the uses technology of becoming thoroughly decomposed.
Summary of the invention
The technical problem to be solved in the present invention: provide a kind of labour intensity low,, without mechanised equipment and the energy, be applicable to bacterial preparation process and the whole strain of maize straw original position that southwest straw decomposing uses and heap become thoroughly decomposed method and structure, can overcome the deficiencies in the prior art.
Technical scheme of the present invention:
A, bacterial strain screening
Get fresh animal waste 1000g, add 4000mL sterilized water, stir 5min, centrifugal 10min, gets supernatant liquor for subsequent use;
B, decomposing microbial inoculum preparation
(1) former bacterial classification preparation: the 30L water of getting 1000g pulverizing filter paper, 80gNa2HPO4,50gKH2PO4,75g peptone and pH6.5 ~ 7.5 is put in fermentor tank, 121 ℃ of sterilizing 30min, be cooled to 55 ℃ ± 0.5 ℃, add the supernatant liquor 3500mL in A step, keep 55 ℃ ± 0.5 ℃, 60 revs/min of stir culture 120 hours, obtain fermented liquid original seed;
(2) prepared by female kind: get cavings, be crushed to crop material, water, urea and fermented liquid original seed below 3mm, 100:10:40:1:1 mixes in mass ratio, by height, 1.0m ~ 1.5m banks up, and in heap postpone every turning in 5 days once, banks up and within 20-25 days, can prepare female kind;
(3) produce bacterial classification preparation: cavings, the crop material, water, urea and the mother that are crushed to below 10mm plant, 100:10:40:1:1 mixes in mass ratio, by height, 1.0m ~ 1.5m banks up, and in heap postpone every turning in 5 days once, banks up and can prepare decomposing microbial inoculum in 25-30 days.
The preparation method of above-mentioned decomposing microbial inoculum is, in steps A, isolates high temperature gemma cellulose-decomposing bacteria as the maize straw bacterial classification that becomes thoroughly decomposed from supernatant liquor.
It is to plant and select smooth region at corn that the method for becoming thoroughly decomposed is heaped in the whole strain of maize straw original position, the whole strain maize straw of collecting after corn is banked up according to the mode anyhow replacing, the total height of maize straw of banking up is 150 cm ~ 180cm, in the process of banking up, in the time that the thickness of banking up reaches 25 cm ~ 35cm, pushed consolidation and spread bacterial classification and urea at surface uniform, bacterial classification consumption is that 1 ‰ of maize straw weight ~ 3 ‰ and amount of urea are 1.5 ‰ of maize straw weight ~ 3 ‰, and every heap maize straw stack weight is not less than 1000kg; Maize straw has been banked up and rear shaping has been carried out in its top, makes its top opposed flattened, then uses plastic sheeting for farm use to cover, and is compressed in fact after covering at top with stone, punches on the plastic sheeting for farm use of pressing stone place simultaneously, keeps the skin wet by rainwater; 90 days ~ 120 days composting time.
The whole strain of maize straw original position is heaped the method for becoming thoroughly decomposed and also can be adopted and after root cuts down, make it be laid in field the maize straw that harvests corn, then get 1.5-2.0kg bacterial classification, 2.5-3.0kg urea, be evenly sprinkled upon on the maize straw after cutting down by every mu, it is become thoroughly decomposed completely.
The structure of becoming thoroughly decomposed is heaped in the whole strain of maize straw original position, it comprises the field of maize planting, be provided with the whole strain maize straw maize straw stacking forming of banking up at the headland in field, the total height of maize straw stacking is 150-180cm, between the maize straw that is 25-35cm at thickness, be provided with decomposing microbial inoculum layer, be coated with plastic sheeting for farm use at maize straw stacking, on the plastic sheeting for farm use at maize straw stacking top, be pressed with stone, have water seepage hole at stone.
It is on the plastic sheeting for farm use of maize straw stacking side, to offer 4-6 breather hole that the structure of becoming thoroughly decomposed is heaped in the whole strain of aforesaid maize straw original position.
It is that whole strain maize straw is banked up according to the mode layering anyhow replacing that the structure of becoming thoroughly decomposed is heaped in the whole strain of above-mentioned maize straw original position.
It is that the top of maize straw stacking is flat-tope structure that the structure of becoming thoroughly decomposed is heaped in the whole strain of aforesaid maize straw original position.
Compared with the prior art,
Innovative point major embodiment of the present invention is in the following areas:
1, the selection of decomposing microbial inoculum, decomposing microbial inoculum adopts high temperature gemma cellulose-decomposing bacteria and urea, high temperature gemma cellulose-decomposing bacteria can increase the quantity of high temperature resistant organism of fermentation, urea can reduce the carbon-nitrogen ratio of maize straw, can meet microorganism Carbon and nitrogen nutrition needs, can pile rotten fertile quality by Integral lifting maize straw;
2, provide the whole strain of a kind of new maize straw original position to heap the method for becoming thoroughly decomposed, the method for banking up, corn plantation ground is selected in the region of banking up of its method, without remote carrying, without agricultural machinery, without heavy labour; Its method adopts the whole strain of maize straw alternately to discharge, without mechanical means, all can complete without the energy, the old and the weak women and children; Its method is without turning, concerning laborer, save time, laborsaving, save worry; Original place carvel built
3, provide the whole strain of a kind of maize straw original position method of becoming thoroughly decomposed, its method is simple, laborsaving to making it naturally be laid in field after root cuts down the maize straw that harvests corn, then bacterial classification, urea is evenly sprinkled upon on the maize straw after cutting down; Though its effect is slightly poor, also can well promote in Southwestern China area;
4, the method for becoming thoroughly decomposed, the whole strain of the maize straw original position method of becoming thoroughly decomposed is heaped in the whole strain of the new maize straw original position proposing, and both operations are very simple, solved the technological difficulties in existing fermenting process, make fermentation technique become " fool " peasant and easily grasp.
Simultaneously for proving the effect of high temperature gemma cellulose-decomposing bacteria, the method (method of becoming thoroughly decomposed is heaped in the whole strain of maize straw original position) that applicant adopts with claim 3 is tested, take Natural compost as reference, (mode of banking up of the maize straw of Natural compost is identical with test method with quantity, but do not cover film, not with microbial inoculum, urea and magnesium sulfate) carry out simultaneous test, content of the test is as follows
1, microbial population and quantity
Table 1 is can culturing micro-organisms population and number change in composting process.Result shows, before test, isolates 14 kinds of bacteriums from maize straw, 7 kinds of fungies, 4 kinds of actinomycetes.In the stalk material of processing in test, the pliotherm period, (35 microbial populations d) significantly reduced, and only isolate 2 strain bacteriums (wherein 1 strain is the high temperature fiber genus bacillus of inoculation), 1 fungal strain and 1 strain actinomycetes; After becoming thoroughly decomposed, (90 d), and microbial population increased than pliotherm period, isolates 9 kinds of bacteriums, 2 kinds of fungies, 2 kinds of actinomycetes.In the stalk material of Natural compost (contrast), the microbial population of pliotherm period than compost before slightly reduce, bacterium, fungi and actinomycetes are respectively 11 kinds, 5 kinds and 3 kinds; Front without significant difference with test while banking up 90 d.
Figure 2013107110046100002DEST_PATH_IMAGE001
2. temperature contrast
As seen from Figure 1, after compost experiment starts, the thermometer of stalk material reveals obvious temperature raising period, pliotherm period, cooldown period.Wherein, approximately 5 ~ 7d of temperature raising period; In the pliotherm period, the temperature of stalk material, between 50 ~ 64 ° of C, approximately maintains 50d; Heap postpone 57d temperature is down to 50 ° below C, 80d after compost, and the temperature of stalk material is down to similar to temperature.But, in Natural compost process, the temperature of stalk material at 5th ~ 70d higher than temperature, but medial temperature than test process low 20.5 ° of C, and fluctuation larger.
3. moisture contrast
Before banking up, the water content of maize straw is 63.4%, and in composting process, the water content of material continues to reduce, and is down to steady state value (23.8% ~ 24.6%) after the 80d that banks up.But in the processing of Natural compost, the water content of precipitation affects material, is generally wave and reduces.Heap postpone 90d, the water content of heap body material is down to 43.1%, is significantly higher than test and processes (Fig. 2).
4.pH contrast
Fig. 3 is visible, and in test is processed, the pH value of stalk material continues to reduce, and the pH value that test is processed is significantly lower than Natural compost.In the time that compost finishes, the pH value of compost material is respectively pH6.14(test and processes) and pH6.42(Natural compost).
5. outward appearance, organic matter and nutrient
Adopt this test to process, along with the increase of the time of banking up, the color of maize straw changes grey, beige, brown, chocolate, volume-diminished into gradually by yellow.While banking up 90 d, maize straw is all chocolate, for chip or Powdered, becomes thoroughly decomposed completely.In the process of Natural compost, while banking up 90 d, maize straw gray or beige, there is tephrosious bacterial plaque on stalk surface, although volume reduces, substantially keeps original form, is difficult to fracture, and becomes thoroughly decomposed not good.
Adopt this test process, while banking up 90 d, the active organic matter of stalk material, nitrogen, Vanadium Pentoxide in FLAKES, potassium oxide content are followed successively by 43.35%(data problem), 1.26%, 0.57% and 1.85%.In Natural compost process, the organic matter of the 90d that banks up, nitrogen, Vanadium Pentoxide in FLAKES, potassium oxide content are followed successively by 33.47%(data problem), 0.72%, 0.46% and 1.74%, all significantly lower than test process.
Can be found out in composting process by above-mentioned test, adopt high temperature gemma cellulose-decomposing bacteria to make organic matter generation mineralising and the humify of material, temperature, moisture, pH and carbon-nitrogen ratio etc. are closely related with microbial activities and compost quality.In test is processed, access high temperature fiber decomposer, can increase the quantity of high temperature resistant organism of fermentation; The carbon-nitrogen ratio of adding urea reduction maize straw, can meet microorganism Carbon and nitrogen nutrition needs; Epiphragma can keep temperature of charge, prevents rainfall ponding, reduces the detrimentally affect of envrionment conditions, and these are all of value to microbial activities, promotes compost fermentation.
At the hot stage of compost, high temperature microbe is the most active, organic mineralising rapidly dehydration; Cooldown period mainly carries out humification, and the two is all critical periods that compost fermentation is produced fertilizer.In test is processed, the temperature of stalk material raises rapidly, shows the obvious pliotherm period, the pliotherm period of 50 ° of C approximately maintains 50 d, be up to 64 ° of C, be not only of value to the rapid mineralising of organic matter and the natural-dehydration of stalk material, and pathogenic microbe killing effectively.After inoculation high temperature fiber bacterium, quick heating, approximately 5 ~ 7 d; In the pliotherm period, the microbial population in stalk material significantly reduces, but microbe population significantly increases, and the overwhelming majority is bacterium.Microbe inoculation accelerated warming is described, the pyrolytic decomposition of stalk organic matter is relevant with high temperature resistant bacterium, and the stalk that microbe inoculation has participated in the pliotherm period decomposes.So, producing in the process of fertilizer at field compost treatment entirety maize straw, inoculation high temperature fiber bacterium has unusual effect.。
In test is processed, material moisture when compost finishes and pH are significantly lower than Natural compost, but organic matter, nitrogen, Vanadium Pentoxide in FLAKES, potassium oxide content are significantly higher than Natural compost, and be chocolate and crumb powder state, illustrate that maize straw is after test is processed, the degree of becoming thoroughly decomposed is better than Natural compost.In tobacco planting process, chemical fertilizer and stalk fertilizer are joined in right amount to execute and are significantly improved yield of flue-cured tobacco and the output value, and improve quality of tobacco.Wherein, 50% chemical fertilizer joins that to execute yield of tobacco, average price and the output value of 50% stalk fertilizer the highest.In addition, join in the processing of executing stalk fertilizer at chemical fertilizer, nicotine content significantly reduces, and tobacco leaf potassium, total reducing sugar, sugared alkali ratio and schmuck value significantly improve, and sugared alkali ratio and schmuck value remain on respectively between 11.80 ~ 12.67 and 4.30 ~ 4.42.The content of the chemical composition of tobacco leaf and ratio thereof are the objective standards of its quality of reflection, directly affect the quality of quality of tobacco.Wherein, potassium can improve the incendivity of tobacco leaf, appropriate sugar make flue gas mouthfeel alcohol and, fragrance is dense, too high protein and nicotine produce assorted taste, pungency increases.The sugar degree of cigarette product improves and increases with grade, and protein mass reduces with grade raising, therefore schmuck value is larger, quality of tobacco is better.Sugar alkali is than the ratio that is total Soluble Sugar and nicotine content, for reacting the soda acid Harmony of tobacco leaf.The sugared alkali of general tobacco leaf is than better in 10 ~ 13 scope.Join in the processing of executing at chemical fertilizer and stalk fertilizer, potassium content in tobacco leaf, total reducing sugar, schmuck value and sugared alkali, than improving, illustrate the coordination of cigarette chemical composition, quality improvement.The soil in Guizhou Province mostly is acidity-neutral yellow earth, and effectively potassium content is generally not high, and the foggy few light of weather, is unfavorable for tobacco leaf sucrose accumulation, uses the alternative chemical fertilizer of maize straw fertilizer, mends potassium and increases sugar, improves quality of tobacco.
In sum, prepare in fertilizer process inoculation high temperature fiber bacterium at field compost treatment entirety maize straw, add urea and magnesium sulfate, covering agricultural film is of value to enhancing microbial activities, promotes organic mineralising and humify, promote that maize straw becomes thoroughly decomposed, improve compost quality.In flue-cured tobacco cultivation, 50% chemical fertilizer is joined and is executed 50% stalk fertilizer and can significantly improve yield of tobacco, average price, the output value, improves quality of tobacco.
accompanying drawing explanation:
Fig. 1 temperature comparison diagram;
Fig. 2 is moisture content comparison diagram;
Fig. 3 pH value comparison diagram;
Fig. 4 is the application's sectional view.
embodiment:
Embodiment 1, the application's first-selection relates to the preparation method of decomposing microbial inoculum, and it comprises the following steps
A, bacterial strain screening
Get fresh animal waste 1000g, add 4000mL sterilized water, stir 5min, centrifugal 10min, gets supernatant liquor for subsequent use;
B, decomposing microbial inoculum preparation
(1) former bacterial classification preparation: the 30L water of getting 1000g pulverizing filter paper, 80gNa2HPO4,50gKH2PO4,75g peptone and pH6.5 ~ 7.5 is put in fermentor tank, 121 ℃ of sterilizing 30min, be cooled to 55 ℃ ± 0.5 ℃, add the supernatant liquor 3500mL in A step, keep 55 ℃ ± 0.5 ℃, 60 revs/min of stir culture 120 hours, obtain fermented liquid original seed;
(2) prepared by female kind: get cavings, be crushed to crop material, water, urea and fermented liquid original seed below 3mm, 100:10:40:1:1 mixes in mass ratio, by height, 1.0m ~ 1.5m banks up, and in heap postpone every turning in 5 days once, banks up and within 20-25 days, can prepare female kind;
(3) produce bacterial classification preparation: cavings, the crop material, water, urea and the mother that are crushed to below 10mm plant, 100:10:40:1:1 mixes in mass ratio, by height, 1.0m ~ 1.5m banks up, and in heap postpone every turning in 5 days once, banks up and can prepare decomposing microbial inoculum in 25-30 days.
Wherein the effective ingredient of decomposing microbial inoculum for isolating high temperature gemma cellulose-decomposing bacteria as the maize straw bacterial classification that becomes thoroughly decomposed in steps A from supernatant liquor.
Preparing after decomposing microbial inoculum, utilizing this decomposing microbial inoculum to carry out the whole strain of maize straw original position heaps the method for becoming thoroughly decomposed and is, select on the spot the field 1 of maize planting, select the smooth headland in field 1, according to the whole strain layering of the mode anyhow the replacing maize straw stacking 2 of banking up, the total height of the maize straw stacking 2 of banking up is 150-180cm, the weight of maize straw stacking 2 is not less than 1000kg, every layer thickness 25-35cm, every layer of maize straw banked up and pushed afterwards consolidation and spread bacterial classification and urea at surface uniform, bacterial classification consumption is that 1 ‰ of maize straw weight ~ 3 ‰ and amount of urea are 1.5 ‰ of maize straw weight ~ 3 ‰, after forming decomposing microbial inoculum layer 3, carry out again banking up of lower floor, maize straw stacking 2 is put to have piled and rear shaping is carried out in its top, makes its opposed flattened, then uses plastic sheeting for farm use 4 to cover, and is compressed in fact after covering at top with stone 5, punches on the plastic sheeting for farm use 4 at pressure stone place simultaneously, keeps the skin wet by rainwater, ventilative for realizing, on the plastic sheeting for farm use of maize straw stacking (2) side, offer breather hole, the quantity of breather hole is every square metre of 4-6, aperture 5mm.The whole compost time is 90 ~ 120 days.
 
Embodiment 2, the application's first-selection relates to the preparation method of decomposing microbial inoculum, and it comprises the following steps
A, bacterial strain screening
Get fresh animal waste 1000g, add 4000mL sterilized water, stir 5min, centrifugal 10min, gets supernatant liquor for subsequent use;
B, decomposing microbial inoculum preparation
(1) former bacterial classification preparation: the 30L water of getting 1000g pulverizing filter paper, 80gNa2HPO4,50gKH2PO4,75g peptone and pH6.5 ~ 7.5 is put in fermentor tank, 121 ℃ of sterilizing 30min, be cooled to 55 ℃ ± 0.5 ℃, add the supernatant liquor 3500mL in A step, keep 55 ℃ ± 0.5 ℃, 60 revs/min of stir culture 120 hours, obtain fermented liquid original seed;
(2) prepared by female kind: get cavings, be crushed to crop material, water, urea and fermented liquid original seed below 3mm, 100:10:40:1:1 mixes in mass ratio, by height, 1.0m ~ 1.5m banks up, and in heap postpone every turning in 5 days once, banks up and within 20-25 days, can prepare female kind;
(3) produce bacterial classification preparation: cavings, the crop material, water, urea and the mother that are crushed to below 10mm plant, 100:10:40:1:1 mixes in mass ratio, by height, 1.0m ~ 1.5m banks up, and in heap postpone every turning in 5 days once, banks up and can prepare decomposing microbial inoculum in 25-30 days.
Wherein the effective ingredient of decomposing microbial inoculum for isolating high temperature gemma cellulose-decomposing bacteria as the maize straw bacterial classification that becomes thoroughly decomposed in steps A from supernatant liquor.
Preparing after decomposing microbial inoculum, utilizing this decomposing microbial inoculum to carry out the whole strain of maize straw original position heaps the method for becoming thoroughly decomposed and is, after cutting down, root make it be laid in field the maize straw that harvests corn, then get 1.5-2.0kg bacterial classification, 2.0-3.0kg urea, be evenly sprinkled upon on the maize straw after cutting down by every mu, it is become thoroughly decomposed completely.

Claims (7)

1. a preparation method for decomposing microbial inoculum, is characterized in that: the method comprises the following steps
A, bacterial strain screening
Get fresh animal waste 1000g, add 4000mL sterilized water, stir 5min, centrifugal 10min, gets supernatant liquor for subsequent use;
B, decomposing microbial inoculum preparation
(1) former bacterial classification preparation: get 1000g and pulverize filter paper, 80gNa 2hPO 4, 50gKH 2pO 4, 75g peptone and pH6.5 ~ 7.5 30L water be put in fermentor tank, 121 ℃ of sterilizing 30min, are cooled to 55 ℃ ± 0.5 ℃, add the supernatant liquor 3500mL in A step, keep 55 ℃ ± 0.5 ℃, 60 revs/min of stir culture 120 hours, obtain fermented liquid original seed;
(2) prepared by female kind: get cavings, be crushed to crop material, water, urea and fermented liquid original seed below 3mm, 100:10:40:1:1 mixes in mass ratio, by height, 1.0m ~ 1.5m banks up, and in heap postpone every turning in 5 days once, banks up and within 20-25 days, can prepare female kind;
(3) produce bacterial classification preparation: cavings, the crop material, water, urea and the mother that are crushed to below 10mm plant, 100:10:40:1:1 mixes in mass ratio, by height, 1.0m ~ 1.5m banks up, and in heap postpone every turning in 5 days once, banks up and can prepare decomposing microbial inoculum in 25-30 days.
2. the preparation method of decomposing microbial inoculum according to claim 1, is characterized in that: in steps A, isolate high temperature gemma cellulose-decomposing bacteria as the maize straw bacterial classification that becomes thoroughly decomposed from supernatant liquor.
3. utilize the whole strain of maize straw original position described in claim 1 to heap the method for becoming thoroughly decomposed, it is characterized in that: the method is to plant and select smooth region at corn, the whole strain maize straw of collecting after corn is banked up according to the mode anyhow replacing, the total height of maize straw of banking up is 150 cm ~ 180cm, in the process of banking up, in the time that the thickness of banking up reaches 25 cm ~ 35cm, pushed consolidation and spread bacterial classification and urea at surface uniform, bacterial classification consumption is that 1 ‰ of maize straw weight ~ 3 ‰ and amount of urea are 1.5 ‰ of maize straw weight ~ 3 ‰, every heap maize straw stack weight is not less than 1000kg, maize straw has been banked up and rear shaping has been carried out in its top, makes its top opposed flattened, then uses plastic sheeting for farm use to cover, and is compressed in fact after covering at top with stone, punches on the plastic sheeting for farm use of pressing stone place simultaneously, keeps the skin wet by rainwater, 90 days ~ 120 days composting time.
4. utilize the whole strain of maize straw original position described in claim 1 method of becoming thoroughly decomposed, it is characterized in that: the method is after root cuts down, to make it naturally be laid in field the maize straw that harvests corn, then by every mu get 1.5kg ~ 2.0kg bacterial classification, 2.0kg ~ 3.0kg urea is evenly sprinkled upon on the maize straw after cutting down, and it is become thoroughly decomposed.
5. the structure of becoming thoroughly decomposed is heaped in the whole strain of maize straw original position, it comprises the field (1) of maize planting, it is characterized in that: corn plantation the headland of (1) be provided with the whole strain maize straw maize straw stacking (2) forming of banking up, the total height of maize straw stacking (2) is 150 cm ~ 180cm, between the maize straw that is 25cm ~ 35cm at thickness, be provided with decomposing microbial inoculum layer (3), be coated with thick plastic sheeting for farm use (4) at maize straw stacking (2), on the thick plastic sheeting for farm use (4) at maize straw stacking (2) top, be pressed with stone (5), have water seepage hole (6) at stone (5).
6. the structure of becoming thoroughly decomposed is heaped in the whole strain of maize straw original position according to claim 5, it is characterized in that: whole strain maize straw is banked up according to the mode layering anyhow replacing.
7. the structure of becoming thoroughly decomposed is heaped in the whole strain of maize straw original position according to claim 5, it is characterized in that: the top of maize straw stacking (2) is flat-tope structure.
CN201310711004.6A 2013-12-20 2013-12-20 Become thoroughly decomposed method and structure are heaped in bacterial preparation process and the whole strain of maize straw original position Expired - Fee Related CN103864481B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310711004.6A CN103864481B (en) 2013-12-20 2013-12-20 Become thoroughly decomposed method and structure are heaped in bacterial preparation process and the whole strain of maize straw original position

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310711004.6A CN103864481B (en) 2013-12-20 2013-12-20 Become thoroughly decomposed method and structure are heaped in bacterial preparation process and the whole strain of maize straw original position

Publications (2)

Publication Number Publication Date
CN103864481A true CN103864481A (en) 2014-06-18
CN103864481B CN103864481B (en) 2015-09-30

Family

ID=50903564

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310711004.6A Expired - Fee Related CN103864481B (en) 2013-12-20 2013-12-20 Become thoroughly decomposed method and structure are heaped in bacterial preparation process and the whole strain of maize straw original position

Country Status (1)

Country Link
CN (1) CN103864481B (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104521520A (en) * 2014-12-31 2015-04-22 吴中区金庭小美华家庭农场 Method for planting tagetes patula
CN105111015A (en) * 2015-08-14 2015-12-02 淮南市家合源大棚蔬菜种植农民专业合作社 Straw composting method
CN106336256A (en) * 2016-08-16 2017-01-18 中国科学院亚热带农业生态研究所 General method for in-situ rapid decomposition of southern crop straw
WO2017118876A1 (en) * 2016-01-05 2017-07-13 Stefan Grass Method for the production of a plant growth substrate from crop residues
CN111165861A (en) * 2020-01-10 2020-05-19 云南乌蒙金叶生物科技有限公司 Tobacco leaf stacking and covering fermentation method
CN111869508A (en) * 2020-07-20 2020-11-03 新乡市达威智能门窗实用技术研究中心 Corn straw oyster mushroom culture material and preparation method and application thereof
CN114045234A (en) * 2021-11-03 2022-02-15 哈尔滨工业大学 Bacterial liquid for promoting aerobic fermentation of corn straw to produce heat, preparation method and application
CN114213161A (en) * 2021-11-30 2022-03-22 四川省农业科学院经济作物育种栽培研究所 Straw joint filling layered decomposition method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1408684A (en) * 2002-09-04 2003-04-09 田艳芬 Straw fast decomposing agent
CN102199564A (en) * 2011-03-28 2011-09-28 武汉合缘绿色生物工程有限公司 Composite microbial agent for direct decomposition of crop straws returned to field and preparation method thereof
CN102433269A (en) * 2011-10-21 2012-05-02 西北农林科技大学 Bacillus and application thereof
CN102838391A (en) * 2011-06-24 2012-12-26 吴杰森 Usage method of straw decomposition agent for straw mulching
CN204079818U (en) * 2013-12-20 2015-01-07 贵州省烟草科学研究院 Structure of becoming thoroughly decomposed is heaped in the whole strain of a kind of maize straw original position

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1408684A (en) * 2002-09-04 2003-04-09 田艳芬 Straw fast decomposing agent
CN102199564A (en) * 2011-03-28 2011-09-28 武汉合缘绿色生物工程有限公司 Composite microbial agent for direct decomposition of crop straws returned to field and preparation method thereof
CN102838391A (en) * 2011-06-24 2012-12-26 吴杰森 Usage method of straw decomposition agent for straw mulching
CN102433269A (en) * 2011-10-21 2012-05-02 西北农林科技大学 Bacillus and application thereof
CN204079818U (en) * 2013-12-20 2015-01-07 贵州省烟草科学研究院 Structure of becoming thoroughly decomposed is heaped in the whole strain of a kind of maize straw original position

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104521520A (en) * 2014-12-31 2015-04-22 吴中区金庭小美华家庭农场 Method for planting tagetes patula
CN104521520B (en) * 2014-12-31 2017-02-15 绍兴胜元环境建设有限公司 Method for planting tagetes patula
CN105111015A (en) * 2015-08-14 2015-12-02 淮南市家合源大棚蔬菜种植农民专业合作社 Straw composting method
WO2017118876A1 (en) * 2016-01-05 2017-07-13 Stefan Grass Method for the production of a plant growth substrate from crop residues
CN106336256A (en) * 2016-08-16 2017-01-18 中国科学院亚热带农业生态研究所 General method for in-situ rapid decomposition of southern crop straw
CN111165861A (en) * 2020-01-10 2020-05-19 云南乌蒙金叶生物科技有限公司 Tobacco leaf stacking and covering fermentation method
CN111869508A (en) * 2020-07-20 2020-11-03 新乡市达威智能门窗实用技术研究中心 Corn straw oyster mushroom culture material and preparation method and application thereof
CN114045234A (en) * 2021-11-03 2022-02-15 哈尔滨工业大学 Bacterial liquid for promoting aerobic fermentation of corn straw to produce heat, preparation method and application
CN114213161A (en) * 2021-11-30 2022-03-22 四川省农业科学院经济作物育种栽培研究所 Straw joint filling layered decomposition method

Also Published As

Publication number Publication date
CN103864481B (en) 2015-09-30

Similar Documents

Publication Publication Date Title
CN103864481B (en) Become thoroughly decomposed method and structure are heaped in bacterial preparation process and the whole strain of maize straw original position
CN103966150B (en) A kind of agricultural crop straw decomposing microbial inoculum and using method thereof and effect
CN104987269A (en) Biological organic fertilizer special for apples and preparation method thereof
CN105493950B (en) A kind of machine-transplanted rice seedling raising method and its application
CN103964946A (en) Special compound microbial fertilizer for paddy rice and preparation method thereof
CN102199564A (en) Composite microbial agent for direct decomposition of crop straws returned to field and preparation method thereof
CN102134169A (en) Technology for producing biological organic fertilizer by using dairy manure and straw
CN102531801A (en) Acidic microbial organic fertilizer and preparation method thereof
CN103518781B (en) Core bio-control bacteria and organic fertilizer containing the core bio-control bacteria
CN102180743A (en) Method for producing insect resistance organic compound fertilizer by fermentation of agricultural organic wastes
CN102674997A (en) Biological fermentation fertilizer and preparation method of biological fermentation fertilizer
CN102887755A (en) Method for preparing organic fertilizer with chicken manure
CN102584467A (en) Biological organic fertilizer prepared by municipal sludge and preparation method thereof
CN102887756A (en) Method for preparing organic fertilizer
CN104355863A (en) Bio-organic fertilizer and preparation method thereof
CN103651067A (en) Soilless turf culturing medium and preparing method of soilless turf culturing medium
CN104126413A (en) Method for producing straw mushrooms by using cassava residues, mulberry stems and sugarcane leaves
CN106588467A (en) Biological fertilizer special for radix sophorae subprostratae and preparation method thereof
CN106591147A (en) Aspergillus niger NJDL-12 bacterial strain and application thereof to improvement of coastal saline-alkali soil
CN108059492A (en) A kind of method that vegetables residuum original position, quick Fertilizer Transformed utilize
CN204079818U (en) Structure of becoming thoroughly decomposed is heaped in the whole strain of a kind of maize straw original position
CN103992192A (en) Microbial compound fertilizer using fermented Zizania aquatica leaves as matrix and preparation method thereof
CN103351188B (en) Preparation method of agricultural organic fertilizer by treating pure straws as raw material
CN105724055B (en) A method of improving agaricus bisporus yield using needle mushroom dreg
CN103044125A (en) Green organic crop bacterial manure and method for producing same

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150930

Termination date: 20161220

CF01 Termination of patent right due to non-payment of annual fee