CN103860624A - Method for removing fructus trichosanthis pulp by assist of biological enzyme - Google Patents
Method for removing fructus trichosanthis pulp by assist of biological enzyme Download PDFInfo
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- CN103860624A CN103860624A CN201410116545.9A CN201410116545A CN103860624A CN 103860624 A CN103860624 A CN 103860624A CN 201410116545 A CN201410116545 A CN 201410116545A CN 103860624 A CN103860624 A CN 103860624A
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- fructus trichosanthis
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- sloughing
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Abstract
The invention relates to a method for removing fructus trichosanthis pulp by assist of a biological enzyme, belongs to the field of medicament producing and processing, and aims at solving the technical problem of providing a method for removing fructus trichosanthis pulp by assist of a biological enzyme, and use of amylase and cellulose for removing the fructus trichosanthis pulp. The method for removing the fructus trichosanthis pulp by assist of the biological enzyme comprises the following steps: putting fructus trichosanthis seeds with pulp into a biological enzyme solution; taking the fructus trichosanthis seeds to wash and dry after separating the fructus trichosanthis seeds from the pulp, so as to obtain the fructus trichosanthis seeds. Furthermore, the invention also discloses use of amylase and cellulose for removing pulp. The method for removing the fructus trichosanthis pulp by assist of the biological enzyme has the advantages of being simple in process, convenient to operate, low in cost, high in removal efficiency and the like.
Description
Technical field
The present invention relates to the auxiliary method of sloughing Fructus Trichosanthis seed of enzyme, belong to drug manufacture manufacture field.
Background technology
Fructus Trichosanthis is the perennial dioecism liana of Cucurbitaceae, the large traditional Chinese medicinal materials assortment of dietotherapeutic, and applicating history is long, and medical value is high, at home and abroad has long enjoyed a good reputation.Fructus Trichosanthis not only has very high medical value; and there is higher nutrition and health care and be worth; its peel, kernel; root is all done medicinal, and often eating Fructus Trichosanthis seed has good effect to protection breathing and cardiovascular system, anticancer, treatment diabetes, treatment " three-hypers " and raising body immune function, adjustment endocrine.
Along with people's going deep into Fructus Trichosanthis understanding, it is worth the accreditation that more and more obtains everybody, Fructus Trichosanthis seed demand on edible, medicinal increases, and in Sichuan, the ground cultivated area such as Anhui, Zhejiang, Shandong is also more and more wider, but the technique that Fructus Trichosanthis seed is peeled off out from Fructus Trichosanthis flesh is but very loaded down with trivial details.Fructus Trichosanthis flesh is tightly bundled together seed, and after peelling off Pericarpium Trichosanthis, Fructus Trichosanthis flesh is wrapped in Fructus Trichosanthis seed tightly, and the many fleshes of seed are hard, and seed obtains very deeply by embedding, therefore wants successfully to peel off fast Fructus Trichosanthis seed and is not easy very much.In production practices, medicinal herb growers conventionally put into the Fructus Trichosanthis seed with Fructus Trichosanthis flesh in woven bag sealing or are covered with plastic sheeting, then water water, make its Fructus Trichosanthis flesh corruption, then after 12～14 days, by Fructus Trichosanthis flesh eluting, obtain Fructus Trichosanthis seed, conventionally adopt and hand-launder or stir and help Fructus Trichosanthis flesh to depart from waddy in container; In cleaning process, Fructus Trichosanthis flesh gives out strong pungent odor, the vomiting that turns one's stomach, and a lot of people's endurables, therefore Fructus Trichosanthis also has a popular name " dung melon seed ".Even if but through above-mentioned processing, Fructus Trichosanthis flesh is still difficult for eluting; Conventionally also need to clean 6～10 times, could be by Fructus Trichosanthis seed eluting out, if hand-laundered, opponent's injury is very large.Therefore the method for traditional eluting Fructus Trichosanthis seed, has consumed a large amount of manpower and materials, has brought great puzzlement to the melon grower of a large amount of plantations.Therefore, finding a kind of simple, convenient, with low cost method of sloughing Fructus Trichosanthis flesh, is problem demanding prompt solution.
Summary of the invention
The technical problem to be solved in the present invention is, provides a kind of auxiliary method of sloughing Fructus Trichosanthis flesh of enzyme and amylase, cellulase for sloughing the purposes of Fructus Trichosanthis flesh.
The auxiliary method of sloughing Fructus Trichosanthis flesh of enzyme of the present invention, is characterized in that comprising the steps: that the Fructus Trichosanthis seed by with flesh puts into enzyme solution, after Fructus Trichosanthis seed separates with flesh, gets Fructus Trichosanthis seed, and washing is dry, obtains Fructus Trichosanthis seed.
Wherein, the enzyme in enzyme solution can be hydrolyzed the organic principle in Fructus Trichosanthis flesh, thereby causes Fructus Trichosanthis flesh to separate with Fructus Trichosanthis seed.
Further, in order to take into account cost and Fructus Trichosanthis flesh is sloughed effect, the auxiliary method of sloughing Fructus Trichosanthis flesh of described enzyme, comprises the steps:
A, by the enzyme solution of putting into flesh Fructus Trichosanthis seed, wherein, be 3～8:3～8g/ml with the solid-liquid ratio of flesh Fructus Trichosanthis seed and enzyme solution, enzyme solution temperature is 25～35 DEG C;
25～35 DEG C of b, maintenance enzyme solution temperatures, leave standstill 60～120h;
C, get Fructus Trichosanthis seed, washing, dry, to obtain final product.
Further, in order to reduce production costs, ensure that Fructus Trichosanthis flesh can effectively be separated, described enzyme solution concentration is preferably 0.05～0.75wt% simultaneously, and more preferably 0.15～0.65wt%, most preferably is 0.25wt%.
Further, in order to enhance productivity, enzyme of the present invention is auxiliary to be sloughed the enzyme adopting in the method for Fructus Trichosanthis flesh and is preferably at least one in amylase, cellulase; Further, described amylase is preferably α-amylase; At least one in the preferred endoglucanase of described cellulase, exoglucanase, beta glucan glycosides enzyme.
Further, enzymatic solution consumption too much can cause the waste of raw material, consumption is crossed and can be affected at least Fructus Trichosanthis flesh and slough effect, in order to accelerate Fructus Trichosanthis flesh decomposition rate, allow the eluting of Fructus Trichosanthis seed cleaner simultaneously, as preferred version, in a step, be 4～7:4～7g/ml with the solid-liquid ratio of flesh Fructus Trichosanthis seed and enzyme solution, be preferably 5:6g/ml.
Further, under above-mentioned steps parameter, in order to make, Fructus Trichosanthis seed and flesh are better to be separated, and in a and b step, enzyme solution temperature is preferably 30 DEG C; In described b step, time of repose is preferably 65～84h, most preferably is 72h; Washing times preferably 2 or 3 times in described c step.
Further, amylase, cellulase are also disclosed in the purposes of sloughing in Fructus Trichosanthis flesh in the present invention; Preferably, described amylase is α-amylase; Described cellulase is at least one in endoglucanase, exoglucanase, beta glucan glycosides enzyme.
The present invention adopts biological enzyme hydrolysis principle to realize, and utilizes the hydrolysis property of enzyme to the organic principle such as starch, saccharide, and the organic principle in Fructus Trichosanthis flesh is decomposed, thereby its flesh seed is separated.
Beneficial effect of the present invention:
1, compared with the method for traditional eluting Fructus Trichosanthis flesh, the present invention utilizes the auxiliary method of sloughing Fructus Trichosanthis flesh of enzyme to Fructus Trichosanthis seed not damaged, and Fructus Trichosanthis seed is more complete.
2, the auxiliary method of sloughing Fructus Trichosanthis flesh of enzyme of the present invention, adopt the enzyme such as amylase, cellulase, by the nutritional labeling in Fructus Trichosanthis flesh as decomposition such as saccharide, starch, celluloses, the degraded of Fructus Trichosanthis flesh, corrupt speed are accelerated, Fructus Trichosanthis flesh is more easily divested, thereby obtains cleaner Fructus Trichosanthis seed.
3, the auxiliary method technique of sloughing Fructus Trichosanthis flesh of enzyme of the present invention is simple, with low cost.
4, the auxiliary softening time of method immersion of sloughing Fructus Trichosanthis flesh of enzyme of the present invention needs 3～5 days at most, and 12～14 days of comparing traditional method, have shortened elution time greatly.
5, enzyme of the present invention is auxiliary sloughs in the method for Fructus Trichosanthis flesh, and washing times is only 2～3 times, compares washing in traditional method 6～10 times, has both simplified operating procedure, has saved the time, has saved again water, has further reduced production cost.
6, Fructus Trichosanthis flesh medical value is high, can be used for extracting beauty treatment, cosmetics, but traditional elution process time is long, and Fructus Trichosanthis flesh all rots, and cannot recycle, and utilizes enzyme process can make Fructus Trichosanthis flesh again utilize, and has increased the added value of Fructus Trichosanthis.
Detailed description of the invention
The auxiliary method of sloughing Fructus Trichosanthis flesh of enzyme of the present invention, is characterized in that comprising the steps: that the Fructus Trichosanthis seed by with flesh puts into enzyme solution, after Fructus Trichosanthis seed separates with flesh, gets Fructus Trichosanthis seed, and washing is dry, obtains Fructus Trichosanthis seed.
Wherein, the enzyme in enzyme solution can be hydrolyzed the organic principle in Fructus Trichosanthis flesh, thereby causes Fructus Trichosanthis flesh to separate with Fructus Trichosanthis seed.
Further, in order to take into account cost and Fructus Trichosanthis flesh is sloughed effect, the auxiliary method of sloughing Fructus Trichosanthis flesh of described enzyme, comprises the steps:
A, by the enzyme solution of putting into flesh Fructus Trichosanthis seed, wherein, be 3～8:3～8g/ml with the solid-liquid ratio of flesh Fructus Trichosanthis seed and enzyme solution, enzyme solution temperature is 25～35 DEG C;
25～35 DEG C of b, maintenance enzyme solution temperatures, leave standstill 60～120h;
C, get Fructus Trichosanthis seed, washing, dry, to obtain final product.
Further, in order to reduce production costs, ensure that Fructus Trichosanthis flesh can effectively be separated, described enzyme solution concentration is preferably 0.05～0.75wt% simultaneously, and more preferably 0.15～0.65wt%, most preferably is 0.25wt%.
Further, in order to enhance productivity, enzyme of the present invention is auxiliary to be sloughed the enzyme adopting in the method for Fructus Trichosanthis flesh and is preferably at least one in amylase, cellulase; Further, described amylase is preferably α-amylase; At least one in the preferred endoglucanase of described cellulase, exoglucanase, beta glucan glycosides enzyme.
Further, enzymatic solution consumption too much can cause the waste of raw material, consumption is crossed and can be affected at least Fructus Trichosanthis flesh and slough effect, in order to accelerate Fructus Trichosanthis flesh decomposition rate, allow the eluting of Fructus Trichosanthis seed cleaner simultaneously, as preferred version, in a step, be 4～7:4～7g/ml with the solid-liquid ratio of flesh Fructus Trichosanthis seed and enzyme solution, be preferably 5:6g/ml.
Further, under above-mentioned steps parameter, in order to make, Fructus Trichosanthis seed and flesh are better to be separated, and in a and b step, enzyme solution temperature is preferably 30 DEG C; In described b step, time of repose is preferably 65～84h, most preferably is 72h; Washing times preferably 2 or 3 times in described c step.
Further, amylase, cellulase are also disclosed in the purposes of sloughing in Fructus Trichosanthis flesh in the present invention; Preferably, described amylase is α-amylase; Described cellulase is at least one in endoglucanase, exoglucanase, beta glucan glycosides enzyme.
The present invention adopts biological enzyme hydrolysis principle to realize, and utilizes the hydrolysis property of enzyme to the organic principle such as starch, saccharide, and the organic principle in Fructus Trichosanthis flesh is decomposed, thereby its flesh seed is separated.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 adopts the inventive method eluting to obtain Fructus Trichosanthis seed
A, 30g is put into the α-amylase solution that 30ml concentration is 0.75wt% with flesh Fructus Trichosanthis seed, wherein, enzymatic solution temperature is 25 DEG C;
25 DEG C of b, maintenance enzymatic solution temperature, leave standstill 60h;
C, get Fructus Trichosanthis seed, washing, dry, obtain Fructus Trichosanthis seed totally.
Embodiment 2 adopts the inventive method eluting to obtain Fructus Trichosanthis seed
A, 30g is put into the endoglucanase solution that 80ml concentration is 0.05wt% with flesh Fructus Trichosanthis seed, wherein, enzymatic solution temperature is 35 DEG C;
35 DEG C of b, maintenance enzymatic solution temperature, leave standstill 120h;
C, get Fructus Trichosanthis seed, washing, dry, obtain Fructus Trichosanthis seed totally.
Embodiment 3 adopts the inventive method eluting to obtain Fructus Trichosanthis seed
A, 50g is put into the enzymatic solution that 60ml concentration is 0.15wt% with flesh Fructus Trichosanthis seed, wherein, in enzymatic solution, enzyme is mixed and is formed by α-amylase, endoglucanase, exoglucanase, beta glucan glycosides enzyme, and enzymatic solution temperature is 30 DEG C;
30 DEG C of b, maintenance enzymatic solution temperature, leave standstill 72h;
C, get Fructus Trichosanthis seed, washing, dry, obtain Fructus Trichosanthis seed totally.
Embodiment 4 adopts the inventive method eluting to obtain Fructus Trichosanthis seed
A, 80g is put into the enzymatic solution that 30ml concentration is 0.65wt% with flesh Fructus Trichosanthis seed, wherein, in enzymatic solution, enzyme is mixed and is formed by α-amylase, exoglucanase, beta glucan glycosides enzyme, and enzymatic solution temperature is 30 DEG C;
30 DEG C of b, maintenance enzymatic solution temperature, leave standstill 84h;
C, get Fructus Trichosanthis seed, washing, dry, obtain Fructus Trichosanthis seed totally.
Embodiment 5 adopts the inventive method eluting to obtain Fructus Trichosanthis seed
A, 40g is put into the enzymatic solution that 70ml concentration is 0.35wt% with flesh Fructus Trichosanthis seed, wherein, the enzyme in enzymatic solution is made up of beta glucan glycosides enzyme and α-amylase, and enzymatic solution temperature is 30 DEG C;
30 DEG C of b, maintenance enzymatic solution temperature, leave standstill 72h;
C, get Fructus Trichosanthis seed, washing, dry, obtain Fructus Trichosanthis seed totally.
Embodiment 6 adopts the inventive method eluting to obtain Fructus Trichosanthis seed
A, be with flesh Fructus Trichosanthis seed to put into enzyme solution 50g, wherein, the cellulase that the α-amylase that enzyme solution is 0.75wt% by 25ml concentration and the concentration of 25ml are 0.25wt% mixes, and enzymatic solution temperature is 30 DEG C; Wherein, described cellulase is the mixture of exoglucanase, beta glucan glycosides enzyme composition;
30 DEG C of b, maintenance enzymatic solution temperature, leave standstill 72h;
C, get Fructus Trichosanthis seed, washing, dry, obtain Fructus Trichosanthis seed totally.
Embodiment 7 adopts the inventive method eluting to obtain Fructus Trichosanthis seed
A, 70g is put into the α-amylase solution that 40ml concentration is 0.75wt% with flesh Fructus Trichosanthis seed, enzymatic solution temperature is 30 DEG C;
30 DEG C of b, maintenance enzymatic solution temperature, leave standstill 72h;
C, get Fructus Trichosanthis seed, washing, dry, obtain Fructus Trichosanthis seed totally.
Embodiment 8 adopts the inventive method eluting to obtain Fructus Trichosanthis seed
A, 50g is put into the cellulase solution that 60ml concentration is 0.25wt% with flesh Fructus Trichosanthis seed, enzymatic solution temperature is 30 DEG C; Wherein, described cellulase is the mixture of endoglucanase, exoglucanase, beta glucan glycosides enzyme composition;
30 DEG C of b, maintenance enzymatic solution temperature, leave standstill 65h;
C, get Fructus Trichosanthis seed, washing, dry, obtain clean Fructus Trichosanthis seed.
Claims (10)
1. the auxiliary method of sloughing Fructus Trichosanthis flesh of enzyme, is characterized in that comprising the steps: that the Fructus Trichosanthis seed by with flesh puts into enzyme solution, after Fructus Trichosanthis seed separates with flesh, gets Fructus Trichosanthis seed, and washing is dry, obtains Fructus Trichosanthis seed.
2. the auxiliary method of sloughing Fructus Trichosanthis flesh of enzyme according to claim 1, is characterized in that comprising the steps:
A, band flesh Fructus Trichosanthis seed being put into enzyme solution, wherein, is 3～8:3～8g/ml with the solid-liquid ratio of flesh Fructus Trichosanthis seed and enzyme solution, and enzyme solution temperature is 25～35 DEG C;
25～35 DEG C of b, maintenance enzyme solution temperatures, leave standstill 60～120h;
C, get Fructus Trichosanthis seed, washing, dry, to obtain final product.
3. the auxiliary method of sloughing Fructus Trichosanthis flesh of enzyme according to claim 1 and 2, is characterized in that, described enzyme solution concentration is 0.05～0.75wt%.
4. the auxiliary method of sloughing Fructus Trichosanthis flesh of enzyme according to claim 3, is characterized in that: described enzyme solution concentration is 0.15～0.65wt%, is preferably 0.25wt%.
5. according to the auxiliary method of sloughing Fructus Trichosanthis flesh of the enzyme described in claim 1～4 any one, it is characterized in that: described enzyme is at least one in amylase, cellulase.
6. the auxiliary method of sloughing Fructus Trichosanthis flesh of enzyme according to claim 5, is characterized in that: described amylase is α-amylase; Described cellulase is at least one in endoglucanase, exoglucanase, beta glucan glycosides enzyme.
7. according to the auxiliary method of sloughing Fructus Trichosanthis flesh of the enzyme described in claim 2～6 any one, it is characterized in that: in described a step, be 4～7:4～7g/ml with the solid-liquid ratio of flesh Fructus Trichosanthis seed and enzyme solution, be preferably 5:6g/ml.
8. according to the auxiliary method of sloughing Fructus Trichosanthis flesh of the enzyme described in claim 2～7 any one, it is characterized in that: in described a and b step, enzyme solution temperature is 30 DEG C; In described b step, time of repose is 65～84h, is preferably 72h; In described c step, washing times is 2 or 3 times.
9. amylase, cellulase are in the purposes of sloughing in Fructus Trichosanthis flesh.
10. amylase according to claim 9, cellulase, in the purposes of sloughing in Fructus Trichosanthis flesh, is characterized in that: described amylase is α-amylase; Described cellulase is at least one in endoglucanase, exoglucanase, beta glucan glycosides enzyme.
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| CN201410116545.9A CN103860624B (en) | 2014-03-26 | 2014-03-26 | Method for removing fructus trichosanthis pulp by assist of biological enzyme |
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| CN201410116545.9A CN103860624B (en) | 2014-03-26 | 2014-03-26 | Method for removing fructus trichosanthis pulp by assist of biological enzyme |
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| CN103860624A true CN103860624A (en) | 2014-06-18 |
| CN103860624B CN103860624B (en) | 2017-04-12 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105287689A (en) * | 2015-11-13 | 2016-02-03 | 四川师范大学 | Preparation method of semen trichosanthis |
| CN106233872A (en) * | 2016-08-04 | 2016-12-21 | 安徽徽大农业有限公司 | A kind of cucumber seeds extracting method |
| CN106957928A (en) * | 2017-03-24 | 2017-07-18 | 南京中医药大学 | A kind of preparation method of snakegourd fructose syrup |
| CN107549748A (en) * | 2017-08-12 | 2018-01-09 | 安徽廷龙食品有限公司 | A kind of method for processing Snakegourd Fruit seed |
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| CN103087548A (en) * | 2013-01-29 | 2013-05-08 | 安阳天尊生物工程有限公司 | Method for extracting trichosanthes kirilowii maxim uranidin |
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2014
- 2014-03-26 CN CN201410116545.9A patent/CN103860624B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103087548A (en) * | 2013-01-29 | 2013-05-08 | 安阳天尊生物工程有限公司 | Method for extracting trichosanthes kirilowii maxim uranidin |
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| 徐储来等: "栝楼栽培与采收加工", 《安徽林业》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105287689A (en) * | 2015-11-13 | 2016-02-03 | 四川师范大学 | Preparation method of semen trichosanthis |
| CN105287689B (en) * | 2015-11-13 | 2019-10-18 | 四川师范大学 | The preparation method of Snakegourd Fruit seed |
| CN106233872A (en) * | 2016-08-04 | 2016-12-21 | 安徽徽大农业有限公司 | A kind of cucumber seeds extracting method |
| CN106957928A (en) * | 2017-03-24 | 2017-07-18 | 南京中医药大学 | A kind of preparation method of snakegourd fructose syrup |
| CN107549748A (en) * | 2017-08-12 | 2018-01-09 | 安徽廷龙食品有限公司 | A kind of method for processing Snakegourd Fruit seed |
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| CN103860624B (en) | 2017-04-12 |
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