CN103087548A - Method for extracting trichosanthes kirilowii maxim uranidin - Google Patents
Method for extracting trichosanthes kirilowii maxim uranidin Download PDFInfo
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- CN103087548A CN103087548A CN2013100334065A CN201310033406A CN103087548A CN 103087548 A CN103087548 A CN 103087548A CN 2013100334065 A CN2013100334065 A CN 2013100334065A CN 201310033406 A CN201310033406 A CN 201310033406A CN 103087548 A CN103087548 A CN 103087548A
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- snakegourd
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- uranidin
- trichosanthes kirilowii
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Abstract
The invention relates to a method for extracting trichosanthes kirilowii maxim uranidin, and solves the problems of dissolving difficulty, extracting difficulty and low extraction rate of the trichosanthes kirilowii maxim uranidin. The method comprises the steps of taking trichosanthes kirilowii maxim, removing seeds and skin; crushing trichosanthes kirilowii crumb, adding deionized water to mix; adjusting a pH value by citric acid; adding pectinase and cellulose; carrying out enzymolysis, filtering in vacuum to obtain a filter cake; adding ethanol to dissolve; bathing in water for 90 minutes at 50 DEG C; filtering to obtain filtrate; concentrating the filtrate by reduced pressure and drying in vacuum. The cell walls of colloid and cellulose are destroyed by a two-enzyme method, so that the uranidin in each cell can be released, so as to easily extract and improve the yield. The viscosity of the extracting solution can be reduced, and the extracting solution is easy to filter.
Description
Technical field
The invention belongs to natural plant pigment and extract preparing technical field, be specifically related to a kind of extracting method of snakegourd yellow pigment.
Background technology
Snake gourd Curcurbitaceae herbaceous perennial vine plant, national most area all has distribution.The yellow pigment that contains xenthophylls and carotene in mature fruit has very strong resistance of oxidation, be also the raw material that is used for the treatment of the ocular fundus pathology medicine simultaneously, research and develop the developing direction of sending out natural, safe, that multi-functional pigment is present food, medicine industry.
Snakegourd is from ancient times to the present extensively planted by China, and its fruit, seed, root all can enter separately medicinal.The skin of mature fruit is distinguished the flavor of sweet, cold in nature, and moistening the lung and resolving the phlegm is arranged, and falls the effect of fiery cough-relieving.Its flesh major ingredient is pectin and cellulose family composition, and with general solvent extraction, most snakegourd yellow pigment is difficult to stripping.
Summary of the invention
Go out for solving snakegourd yellow pigment indissoluble, be difficult for extracting, the problem that extraction yield is low the invention provides a kind of extracting method of snakegourd yellow pigment.
technical scheme of the present invention is: a kind of extracting method of snakegourd yellow pigment, get snakegourd, remove seed, peeling, with the snakegourd flesh, pulverize, get feed liquid, add deionized water, the weightmeasurement ratio of feed liquid and deionized water is that (described bulking value refers to that feed liquid is in g to 1:15, deionized water is in ml), after mixing, be 10% citric acid adjust pH=4.0～6.0 with mass concentration again, get compound, add the polygalacturonase of feed liquid weight 0.1～0.5% and the cellulase of feed liquid weight 0.3-1.0%, then, enzymolysis is 3 hours under the condition of 50 ℃～60 ℃, vacuum filtration, get filter cake, filter cake is namely the pigment part, adding mass concentration is 95% dissolve with ethanol, the weightmeasurement ratio of feed liquid and 95% ethanol is that (described bulking value refers to that feed liquid is in g to 1:20, 95% ethanol is in ml), 50 ℃ of water-baths 90 minutes, filter, get filtrate, filtrate decompression is concentrated, vacuum-drying, and get final product.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
The beneficial effect that the present invention is obtained
The present invention makes with double-enzyme method that in the snakegourd flesh, 90% water-fast pigment fully discharges, and solves the very low defective of traditional extraction process extraction yield.
It is mainly colloid and cellulosic material that the snakegourd flesh goes after seed, and pigment is wrapped wherein.The present invention adopts double-enzyme method to destroy colloid and cellulosic cell walls, the interior yellow pigment of cell can be discharged, thereby be easy to extract, and improves yield.And can reduce the viscosity of extracting solution, easily filter.
The invention solves the problem that in the snakegourd flesh, yellow pigment is difficult to stripping, pectin substance in flesh, fibrination is wrapped in pigment, causes the low technical barrier of extraction yield of snakegourd yellow pigment.
Specific embodiment
Embodiment 1
get snakegourd, remove seed, peeling, the snakegourd flesh is put into food-liquid grinder to be pulverized, get feed liquid, take feed liquid 10g, add the 150ml deionized water, after mixing, be that 10% citric acid is adjusted to pH value=4.0 with mass concentration again, get compound, then the cellulase that adds 0.05g polygalacturonase and 0.1g, under the condition of 50 ℃, enzymolysis is 3 hours, vacuum filtration, filter cake is namely the pigment part, adding the 200ml mass concentration is 95% dissolve with ethanol, put in water-bath, 50 ℃ were heated 90 minutes, remove by filter impurity, get filtrate 1ml, be that 95% ethanol is settled to 25ml with mass concentration, at the 438nm place, survey its light absorption value A=0.72, after the residual filtrate concentrating under reduced pressure reclaims ethanol, vacuum-drying gets finished product 1.1g, one time extraction yield is 11%.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
Embodiment 2
get snakegourd, peeling, the snakegourd flesh is put into food-liquid grinder to be pulverized, get feed liquid, take feed liquid 10g, add the 150ml deionized water, after mixing, be that 10% citric acid is adjusted to pH value=5.3 with mass concentration again, get compound, then the cellulase that adds 0.03g polygalacturonase and 0.07g, under the condition of 60 ℃, enzymolysis is 3 hours, vacuum filtration, filter cake is namely the pigment part, adding the 200ml mass concentration is 95% dissolve with ethanol, put in water-bath, 50 ℃ were heated 90 minutes, remove by filter impurity, get filtrate 1ml, be that 95% ethanol is settled to 25ml with mass concentration, at the 438nm place, survey its light absorption value A=0.65, after the residual filtrate concentrating under reduced pressure reclaims ethanol, vacuum-drying, 0.89g gets product, one time extraction yield is 8.9%.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
Embodiment 3
get snakegourd, remove seed, peeling, the snakegourd flesh is put into food-liquid grinder to be pulverized, get feed liquid, take feed liquid 10g, add the 150ml deionized water, after mixing, be that 10% citric acid is adjusted to pH value=6.0 with mass concentration again, then the cellulase that adds 0.01g polygalacturonase and 0.03g, under the condition of 55 ℃, enzymolysis is 3 hours, vacuum filtration, filter cake is namely the pigment part, adding the 200ml mass concentration is 95% dissolve with ethanol, put in water-bath, 50 ℃ were heated 90 minutes, remove by filter impurity, filter, get filtrate 1ml, be that 95% ethanol is settled to 25ml with mass concentration, at the 438nm place, measure its light absorption value A=0.66, after the residual filtrate concentrating under reduced pressure, vacuum-drying gets finished product 0.92g, one time extraction yield is 9.2%.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
Comparison example
Get snakegourd flesh slag and put into food-liquid grinder and pulverize, take snakegourd flesh slurry 10g, add deionized water by the solid-liquid ratio of 1g:15ml, be adjusted to PH=5.8 with 10% citric acid, 50 ℃ ~ 60 ℃ heating in water bath 30 hours, cooling, lose pigment and separate out, vacuum filtration, sad filter.Filter cake takes out puts into 500ml tool plug triangular flask, the 50 ℃ of water-baths of ethanol 90 minutes that add 200ml95%, filter, get filtrate 1ml, the ethanol with 95% is settled to 25ml, at the 438nm place, measure its light absorption value A=0.37, after the residual filtrate concentrating under reduced pressure, vacuum-drying gets finished product 0.56g, and one time extraction yield is 5.6%.
From above embodiment and Comparative Examples, we can find out advantage of the present invention, and the present invention selects suitable enzyme dosage, PH, solid-liquid ratio, temperature and enzymolysis time are easy to filter operation, and extraction yield is high, than without double-enzyme method, directly improve nearly one times of yield with 95% extraction using alcohol.
Claims (3)
1. the extracting method of a snakegourd yellow pigment, it is characterized in that: get snakegourd, remove seed, peeling, with the snakegourd flesh, pulverize, get feed liquid, add deionized water, the weightmeasurement ratio of feed liquid and deionized water is 1:15, after mixing, be 10% citric acid adjust pH=4.0～6.0 with mass concentration again, get compound, add the polygalacturonase of feed liquid weight 0.1～0.5% and the cellulase of feed liquid weight 0.3-1.0%, then, enzymolysis is 3 hours under the condition of 50 ℃～60 ℃, vacuum filtration, get filter cake, filter cake is namely the pigment part, adding mass concentration is 95% dissolve with ethanol, the weightmeasurement ratio of feed liquid and 95% ethanol is 1:20, 50 ℃ of water-baths 90 minutes, filter, get filtrate, filtrate decompression is concentrated, vacuum-drying, and get final product.
2. the extracting method of snakegourd yellow pigment according to claim 1, is characterized in that, the enzyme activity of described polygalacturonase is 50,000 u/g.
3. the extracting method of snakegourd yellow pigment according to claim 1, is characterized in that, the enzyme activity of described polygalacturonase is 10,000 u/g.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103815509A (en) * | 2014-02-22 | 2014-05-28 | 彭常安 | Preparation method of Trichosanthes kirilowii maxim seed beverage |
| CN103860624A (en) * | 2014-03-26 | 2014-06-18 | 成都理工大学 | Method for removing fructus trichosanthis pulp by assist of biological enzyme |
| CN103923482A (en) * | 2014-04-10 | 2014-07-16 | 福建新华东食品有限公司 | Method for extracting sea crab shell haematochrome |
| CN105238097A (en) * | 2015-11-06 | 2016-01-13 | 吉首大学 | Method for supercritical carbon dioxide extraction of green pigment in snakegourd seeds and pulp |
| CN109880400A (en) * | 2019-03-25 | 2019-06-14 | 潜山市天宝农业开发有限公司 | A kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit |
| CN111317691A (en) * | 2020-02-12 | 2020-06-23 | 南京中医药大学 | Trichosanthes kirilowii flesh total yellow pigment with whitening effect and preparation method and application thereof |
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| CN102166038A (en) * | 2011-03-15 | 2011-08-31 | 深圳波顿香料有限公司 | Method for extracting snakegourd peel, application of snakegourd peel extract to cigarettes and cigarettes |
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- 2013-01-29 CN CN201310033406.5A patent/CN103087548B/en not_active Expired - Fee Related
Patent Citations (1)
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| CN102166038A (en) * | 2011-03-15 | 2011-08-31 | 深圳波顿香料有限公司 | Method for extracting snakegourd peel, application of snakegourd peel extract to cigarettes and cigarettes |
Non-Patent Citations (2)
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| 孙体健等: "栝楼黄色素的提取及性能研究", 《中国食品卫生杂志》, vol. 17, no. 03, 30 May 2005 (2005-05-30), pages 228 - 231 * |
| 文廷刚等: "瓜蒌皮色素提取方法及其理化性质研究", 《食品科学》, vol. 30, no. 05, 1 March 2009 (2009-03-01), pages 32 - 35 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103815509A (en) * | 2014-02-22 | 2014-05-28 | 彭常安 | Preparation method of Trichosanthes kirilowii maxim seed beverage |
| CN103815509B (en) * | 2014-02-22 | 2016-01-20 | 南陵智联电子科技有限公司 | A kind of preparation method of Seeds of Trichosanthes kirilowii beverage |
| CN103860624A (en) * | 2014-03-26 | 2014-06-18 | 成都理工大学 | Method for removing fructus trichosanthis pulp by assist of biological enzyme |
| CN103860624B (en) * | 2014-03-26 | 2017-04-12 | 成都理工大学 | Method for removing fructus trichosanthis pulp by assist of biological enzyme |
| CN103923482A (en) * | 2014-04-10 | 2014-07-16 | 福建新华东食品有限公司 | Method for extracting sea crab shell haematochrome |
| CN103923482B (en) * | 2014-04-10 | 2016-02-10 | 福建新华东食品有限公司 | The extracting method of Sea crab crab shell haematochrome |
| CN105238097A (en) * | 2015-11-06 | 2016-01-13 | 吉首大学 | Method for supercritical carbon dioxide extraction of green pigment in snakegourd seeds and pulp |
| CN109880400A (en) * | 2019-03-25 | 2019-06-14 | 潜山市天宝农业开发有限公司 | A kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit |
| CN111317691A (en) * | 2020-02-12 | 2020-06-23 | 南京中医药大学 | Trichosanthes kirilowii flesh total yellow pigment with whitening effect and preparation method and application thereof |
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| CN103087548B (en) | 2014-10-22 |
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