CN103848504B - Biological reinforced dose and preparation method thereof and using method - Google Patents
Biological reinforced dose and preparation method thereof and using method Download PDFInfo
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Abstract
The invention belongs to environmental engineering trade effluent biologic treating technique field, be specially biological reinforced dose of one and preparation method thereof and using method, join in trade effluent biological reaction tank, to remove the organic pollutant in waste water.The present invention according to microorganism growth to nutraceutical demand, develop a kind of nontoxic and containing biological reinforced dose of bacterial classification, this medicament contains the compositions such as humic acids, vitamin H, amino acid, phytokinin, VITAMIN, trace element, drop in polluted water body, energy accelerate growth of microorganism, makes its amount reproduction, activates the activity of indigenous microorganism, by the Co metabolism effect of microorganism, remove the pollutent in water.This biological reinforced dose of raw material is cheap and easy to get, and preparation process is easy, and medicament energy long-time stable is preserved.Adopt this biological reinforced dose of auxiliary process as Industrial Wastewater Treatment, easy and simple to handle, energy-efficient, working cost is low, can be used in all technique utilizing biological method process trade effluent, has broad application prospects.
Description
Technical field
The present invention relates to a kind of medicament of Industrial Wastewater Treatment, namely develop a kind of nontoxic and not containing biological reinforced dose of bacterial classification and preparation method thereof and using method, join in trade effluent biological reaction tank, to remove the organic pollutant in waste water.The invention belongs to environmental engineering trade effluent biologic treating technique field, belong to biological reinforcing technology category.
Background technology
Bioremediation technology is the emerging technology that development in recent years is got up, and due to its reduced investment, does not cause the features such as secondary pollution to become and develops Treatment process rapidly.Microorganism remediation technology mainly contains two kinds of forms: one is utilize indigenous microorganism, for indigenous microorganism provides suitable living environment, as sewage disposal, for it provides the condition such as dissolved oxygen, nutritive substance, suitable pH value, temperature, to promote the growth of indigenous microorganism to degrade environmental pollutant, thus environment purification; Two is screening specified strains, for specific pollutent, filters out the microbial population of this pollutant of can degrading, specific engineering bacteria is inoculated in contaminated environment, and the life condition adapted to most is provided for it, to degradable pollutent, reach the object of environment remediation.The reparation of comparison Physicochemical, microorganism remediation major advantage has that less investment, energy consumption are low, convenient management, the advantage such as safe and reliable, meets economy type of setting up, resource recycling sustainable development society theory.
Biological reinforcing technology is the one of biological restoration, by adding nutritive substance, nontoxic surfactants, electron acceptor(EA) or co-metabolic substance in contaminated environment, activate the microorganism with degradation of contaminant ability that in contaminated environment, itself exists, its activity is greatly enhanced, quick growth and breeding, thus remove pollutent.
The microorganism existed in the environment all needs, from external world's picked-up matter and energy, meet the needs of own growth and breeding, and the nutrition of microorganism growth and energy mainly to originate from six large key elements such as carbon source, nitrogenous source, somatomedin, the energy, inorganic salt and water.In contaminated environment, pollutent only provides a certain in the large key element of carbon source or nitrogenous source etc. six or certain is several, and cause microorganism growth nutritive deficiency, nutraceutical shortage is usually the main limiting factor of microorganisms in water degradation of contaminant.Part nutritive substance or the biotechnological formulation containing some nutritive substance is added in contaminated environment, or add tensio-active agent, for microorganism growth provides nutrition, promote that it is to the absorption of organic pollutant, be conducive to its Growth and reproduction, thus consumption pollutent, contaminant degradation is become carbonic acid gas and water, the object reaching repairing environment improves water body purification efficiency simultaneously.
Typically biological reinforced dose generally contains organic acid, phytokinin, vitamin H, monose, natural hormone, enzyme, natural surface active agent, toxicity buffer reagent, trace element etc.Biological reinforced dose is added in industrial wastewater treatment system, the metabolism of microorganism in Waste Water Treatment can be promoted, impel microorganism to grow in poor environment rapid, high volume, form good zoogloea, Co metabolism effect is formed to organic hard-degraded substance, improves water treatment effect.Meanwhile, biological reinforced dose can also increase microbial diversity, multiple-microorganism is more effectively worked in coordination with and plays a role, degradation of contaminant more up hill and dale, and improve system resistance to overload shock ability.
Biological growth-promoting technology is studied comparatively early abroad, practical engineering application is also many, largest is once 1989, " Alaska research project " that the U.S. implements, for repairing the petroleum pollution in prince William bay, Alaska, added nitrogenous source and phosphorus source wherein as nutritive substance, make petroleum hydrocarbon degradation bacterium add 1-2 the order of magnitude, whole project is carried the first two months and is completed.Pharmaceutical preparation containing slow releasing pharmaceutical fertilizer, lactic acid and yeast extract paste etc. joins in the anaerobic sludge being subject to AH pollution by the people such as Quang-Dung Bach, PAH degradation rate is made to improve 1.2-8.2 doubly, after explanation adds this stimulation medicament, have promoter action to indigenous microorganism Growth and reproduction, indigenous microorganism quantity significantly improves.
Biological growth-promoting technology is as a kind of emerging pollution treatment means, represent current comparatively advanced biotechnology form and technical concept, possess skills the feature such as advanced person, economical and energy saving (without capital construction, non-power consumption, less investment), convenient management (simple to operate, without complicated utility appliance, without the need to special manning) and safe and reliable (utilize and strengthen the natural law of degradation of pollutant, do not increase other pollution form pollution treatment while).
Summary of the invention
The object of this invention is to provide biological reinforced dose of one and preparation method thereof and using method, this the biological reinforced dose auxiliary process that can be used as Industrial Wastewater Treatment, namely add in a certain amount of biological reinforced dose of trade effluent biological treatment facility extremely run or the biological treatment system being on the verge of to collapse, for the microorganism in system provides energy and nutritive substance, excite the activity of indigenous microorganism in sewage, the degradation capability of artificial-strengthening microorganism, accelerate organic degradation process, reach better wastewater treatment efficiency, improve the stability of biological treatment system simultaneously, the capacity of resisting impact load of system can be improved, contribute to the fast quick-recovery of collapse system.Adopt this biological reinforced dose to strengthen Industrial Wastewater Treatment, treatment process be simple, less investment, do not consume energy consumption, treatment effect is good.
Technical scheme of the present invention is:
A kind of biological reinforced dose, containing materials such as peptone, amino acid, glucose, VITAMIN, phytokinin, vitamin H, humic acids, trace elements, the massfraction of each material is:
Peptone 3.0%-5.2%; Amino acid 2.0%-4.5%; Glucose 1.4%-2.7%; VITAMIN 1.2%-2.5%; Phytokinin 0.27%-0.56%; Vitamin H 0.012%-0.037%; Humic acids 0.75%-1.5%; Trace element 0.5-1.5%; Surplus is the sodium chloride aqueous solution of concentration 0.5wt%.
In the present invention, trace element is inorganic nutrient substance, is mainly mineral nutrition and comprises phosphorus, sulphur, potassium, calcium, magnesium, iron, manganese, copper, cobalt, zinc and molybdenum etc., and ratio shared separately in trace element is not limit.Its effect, mainly as the composition of cell and the activator of enzymic activity, has promoter action to reaction each in microbe.
Preferably, in trace element, parts by weight shared are separately respectively phosphorus 10-30 part, sulphur 3-15 part, potassium 5-10 part, calcium 5-10 part, magnesium 1-3 part, iron 0.3-0.5 part, manganese 0.2-0.4 part, copper 0.2-0.5 part, cobalt 0.1-0.3 part, zinc 0.1-0.3 part, molybdenum 0.1-0.2 part.
The biological reinforced medicament safety non-toxic of the present invention, not containing bacterial classification, pH near neutral, for light brown liquid, containing the nutritive substance needed for microorganism growth, its nutritive ingredient contains: the accelerate growth of microorganisms such as vitamin H, phytokinin, amino acid, humic acids, monose, VITAMIN, trace element, the composition of activating microorganisms activity.By repetition test, improved formulations, containing materials such as peptone, humic acids, glucose, vitamin H, amino acid, VITAMIN, phytokinin, sodium-chlor, trace elements, according to the mixing of certain ratio, is mixed with biological reinforced dose.This medicament is dropped in sewage, can breed by accelerate growth of microorganism, excite microbic activity, by the Co metabolism effect of microorganism, to remove Recalcitrant chemicals in trade effluent, improve system stability, accelerate debugging biological treatment speed.
In the present invention, peptone is by the pulvis dry after protease hydrolysis such as fish, meat, be rich in organic nitrogen compound and some VITAMIN, can provide the nutritive substances such as carbon source, nitrogenous source and somatomedin for microorganism growth and breeding, some vitamin H contained can have promoter action to the specific meta-bolites of microorganism secretion.
In the present invention, VITAMIN is one or more of the category-B such as VITMAIN B1, B3, B5 VITAMIN, and its effect mainly stimulates microorganism to produce specific enzymes class, thus enhancement microbiological decomposes and utilizes organic hard-degraded substance.
The preparation method of described biological reinforced dose, is dissolved in component in the sodium chloride solution of 0.5wt%, is light brown liquid by the medicament stirring, leave standstill, the operation such as filtration is mixed with, pH value near neutral 6.8-7.2.The step of preparation is as follows:
The first step: dissolve.Take the materials such as peptone, amino acid, glucose, VITAMIN, phytokinin, vitamin H, humic acids, trace element according to described ratio, load weighted material being added respectively concentration is in the sodium chloride solution of 0.5wt%, makes mixed solution;
Second step: stir.Mixing solutions is slowly stirred 2-4 hour, reaches Homogeneous phase mixing;
3rd step: leave standstill.Solution after stirring at room temperature leaves standstill 4-6 hour;
4th step: filter.Filtered by mixed solution after leaving standstill, the filtrate obtained is biological reinforced dose of preparation.
In the present invention, the biological reinforced dose of light sealing that becomes, can long-time stable preserve.
The using method that the present invention is biological reinforced dose, before adding biological reinforced dose, according to trade effluent self character determination dosage, by biological reinforced dilution agent 10-20 doubly, adds equalizing tank or the water inlet of Sewage treatment systems.
Advantage of the present invention is as follows:
1. cheaper starting materials of the present invention is easy to get;
2. process for preparation of the present invention is simple, is easy to store;
3. the present invention uses the process of biological reinforced dose simple, can adopt and manually add, without the need to energy consumption, not increase off-set facility, drop into without any capital construction, make full use of original sewage treatment facility.
4. the present invention is in sewage treatment project, add the biological reinforced dose of auxiliary process as sewage disposal, processing cost is low, the cost that sewage per ton increases is only 0.3-0.6 unit,, sludge bulking significant sewage many to difficult degradation, foul smelling, foam has good treatment effect, improves efficiency and the economy for the treatment of system.
5. the present invention can be used in all technique utilizing biological method process trade effluent, has wide range of applications.
Embodiment
Embodiment 1: the high-concentration waste water of process ShaoXing,ZheJiang printing and dyeing mill
In the present embodiment, biological reinforced dose contains the materials such as peptone, amino acid, glucose, VITAMIN, phytokinin, vitamin H, humic acids, trace element, and the massfraction of each material is:
Peptone 4%; Amino acid 3%; Glucose 1.8%; VITAMIN (the present embodiment is B1) 1.6%; Phytokinin 0.35%; Vitamin H 0.020%; Humic acids 1.1%; Trace element 1%; Surplus is the sodium chloride aqueous solution of concentration 0.5wt%.Trace element is inorganic nutrient substance, be mainly mineral nutrition and comprise phosphorus, sulphur, potassium, calcium, magnesium, iron, manganese, copper, cobalt, zinc and molybdenum etc., in trace element, parts by weight shared are separately respectively 23 parts, phosphorus, sulphur 11 parts, 6.5 parts, potassium, calcium 5.2 parts, 2.3 parts, magnesium, iron 0.37 part, 0.28 part, manganese, copper 0.26 part, cobalt 0.17 part, 0.12 part, zinc, molybdenum 0.11 part.
The step of preparation is as follows:
The first step: dissolve.Take the materials such as peptone, amino acid, glucose, VITAMIN, phytokinin, vitamin H, humic acids, trace element according to described ratio, load weighted material being added respectively concentration is in the sodium chloride solution of 0.5wt%, makes mixed solution;
Second step: stir.Mixing solutions is slowly stirred 3 hours, reaches Homogeneous phase mixing;
3rd step: leave standstill.Solution after stirring at room temperature leaves standstill 5 hours;
4th step: filter.Filtered by mixed solution after leaving standstill, the filtrate obtained is biological reinforced dose of preparation.
This sewage work adopts acidication+Biological Contact Oxidation Process, treatment scheme is: raw waste water-equalizing tank-UBF acidication-bio-contact oxidation-second pond-air flotation pool-water outlet, effluent quality requires to reach " integrated wastewater discharge standard " (GB 8978-1996) three grades of emission standards, enter in municipal sewage plant, by biological reinforced dilution agent 10 times, in equalizing tank, add 10ml/m
3biological reinforced dose of sewage, by detecting Inlet and outlet water water-quality guideline, and contrast does not add the sewage disposal water quality index of biological reinforced dose, investigates the biological reinforced dose of impact on Sewage treatment systems water quality.Water-quality guideline is as shown in table 1, table 2.
1) biological reinforced dose is not added
Table 1 does not add the Sewage treatment systems Inlet and outlet water water-quality guideline of biological reinforced dose
| Index | COD Cr | Colourity | Ammonia nitrogen | Total phosphorus |
| Water inlet (mg/l) | 4320 | 400 | 24.8 | 2.6 |
| Water outlet (mg/l) | 1090 | 128 | 17.5 | 0.8 |
| Removal rate (%) | 74.7 | 68 | 29.4 | 69.2 |
2) biological reinforced dose is added
Table 2 adds the Sewage treatment systems Inlet and outlet water water-quality guideline of biological reinforced dose
| Index | COD Cr | Colourity | Ammonia nitrogen | Total phosphorus |
| Water inlet (mg/l) | 4286 | 420 | 23.1 | 4.0 |
| Water outlet (mg/l) | 482 | 66 | 9.6 | 0.3 |
| Removal rate (%) | 88.8 | 84.3 | 58.4 | 92.5 |
After adding biological reinforced dose, compared with the treatment effect do not added, COD
cr, colourity, ammonia nitrogen and total phosphorus removal rate greatly increase, simultaneously because dyeing waste water variation water quality is comparatively large, before adding biological reinforced dose, the fluctuation of biological treatment system effluent quality is comparatively large, add biological illustrate biological reinforced dose after, system stability is strengthened.Illustrate and add the processing power to dyeing waste water and the efficiency that this biological reinforced dose can be improved system, consolidation system stability.
Embodiment 2: the waste water of process Shanghai pharmaceutical factory
In the present embodiment, biological reinforced dose contains the materials such as peptone, amino acid, glucose, VITAMIN, phytokinin, vitamin H, humic acids, trace element, and the massfraction of each material is:
Peptone 3.0%; Amino acid 4.5%; Glucose 1.4%; VITAMIN (the present embodiment is B5) 2.5%; Phytokinin 0.27%; Vitamin H 0.037%; Humic acids 0.75%; Trace element 1.5%; Surplus is the sodium chloride aqueous solution of concentration 0.5wt%.Trace element is inorganic nutrient substance, be mainly mineral nutrition and comprise phosphorus, sulphur, potassium, calcium, magnesium, iron, manganese, copper, cobalt, zinc and molybdenum etc., in trace element, parts by weight shared are separately respectively 20 parts, phosphorus, sulphur 10 parts, 6.4 parts, potassium, calcium 7.2 parts, 1.4 parts, magnesium, iron 0.47 part, 0.22 part, manganese, copper 0.31 part, cobalt 0.1 part, 0.14 part, zinc, molybdenum 0.11 part.
The step of preparation is as follows:
The first step: dissolve.Take the materials such as peptone, amino acid, glucose, VITAMIN, phytokinin, vitamin H, humic acids, trace element according to described ratio, load weighted material being added respectively concentration is in the sodium chloride solution of 0.5wt%, makes mixed solution;
Second step: stir.Mixing solutions is slowly stirred 2 hours, reaches Homogeneous phase mixing;
3rd step: leave standstill.Solution after stirring at room temperature leaves standstill 4 hours;
4th step: filter.Filtered by mixed solution after leaving standstill, the filtrate obtained is biological reinforced dose of preparation.
This biochemical treatment process for wastewater is: raw wastewater-equalizing tank-hydrolysis acidification pool-UASB anaerobic pond-biological contact oxidation pond-second pond-water outlet.In actual motion, owing to containing organic pollutant excessive concentration in waste water, reach more than 20000mg/l, biochemical treatment facility cannot normally run.In aerobic treatment process, also create serious foul gas, affect surrounding enviroment.Influent COD
crfor 22000-24000mg/l, water outlet COD
crfor 2400-2700mg/l, processing efficiency about 80%, can not reach " integrated wastewater discharge standard " (GB 8978-1996) three grades of emission standards.
By biological reinforced dilution agent 10 times, biological reinforced dose of 30ml/m3 sewage is added at the equalizing tank water-in of Waste Water Treatment, influent COD Cr is 23000mg/l, water outlet CODCr is about 500mg/l, processing efficiency is 97.8%, with do not add compared with in the of biological reinforced dose, processing efficiency improves 17%, and essentially eliminates stench and greatly reduce a large amount of foams occurred in aeration process.
Embodiment 3: process Shanghai livestock breeding wastewater
In the present embodiment, biological reinforced dose contains the materials such as peptone, amino acid, glucose, VITAMIN, phytokinin, vitamin H, humic acids, trace element, and the massfraction of each material is:
Peptone 5.2%; Amino acid 2.0%; Glucose 2.7%; VITAMIN (the present embodiment is B3:B5 weight ratio=1:1) 1.2%; Phytokinin 0.56%; Vitamin H 0.012%; Humic acids 1.5%; Trace element 0.5%; Surplus is the sodium chloride aqueous solution of concentration 0.5wt%.Trace element is inorganic nutrient substance, be mainly mineral nutrition and comprise phosphorus, sulphur, potassium, calcium, magnesium, iron, manganese, copper, cobalt, zinc and molybdenum etc., in trace element, parts by weight shared are separately respectively 11 parts, phosphorus, sulphur 3 parts, 7.2 parts, potassium, calcium 5.4 parts, 2.6 parts, magnesium, iron 0.47 part, 0.38 part, manganese, copper 0.46 part, cobalt 0.27 part, 0.22 part, zinc, molybdenum 0.17 part.
The step of preparation is as follows:
The first step: dissolve.Take the materials such as peptone, amino acid, glucose, VITAMIN, phytokinin, vitamin H, humic acids, trace element according to described ratio, load weighted material being added respectively concentration is in the sodium chloride solution of 0.5wt%, makes mixed solution;
Second step: stir.Mixing solutions is slowly stirred 4 hours, reaches Homogeneous phase mixing;
3rd step: leave standstill.Solution after stirring at room temperature leaves standstill 6 hours;
4th step: filter.Filtered by mixed solution after leaving standstill, the filtrate obtained is biological reinforced dose of preparation.
This waste water treatment process is: solid-liquid separation-UASB(up-flow anaerobic sludge blanket)-anaerobic pond, water outlet performs " livestock and poultry breeding industry pollutant emission standard " (GB 18596-2001).By biological reinforced dilution agent 20 times, biological reinforced dose of 30ml/m3 sewage is added at equalizing tank water-in, by detecting Inlet and outlet water water-quality guideline, and contrast does not add the sewage disposal water quality index of biological reinforced dose, investigates the biological reinforced dose of impact on livestock breeding wastewater treatment system water quality.Water-quality guideline is as shown in table 3.
Table 3 leachate treatment system Inlet and outlet water water-quality guideline
After adding biological reinforced dose, COD
crincrease to some extent with ammonia nitrogen removal rate, substantially the emission standard that " livestock and poultry breeding industry pollutant emission standard " (GB 18596-2001) specifies can be reached, and do not need additionally to increase water processing establishment and energy consumption, visible employing biological reinforced dose of auxiliary process as livestock breeding wastewater treatment system is a kind of economically viable means.
Containing the organic pollutant of high density in most industry waste water, a lot of pollutent belongs to hard-degraded substance, carries out a biological disposal upon to be merely able to some organic pollutants of degrading within the regular hour, causes water outlet pollutant load still very high.In biologic treating technique while microbiological deterioration organic pollutant, biogenic energy and material need be absorbed from outside atmosphere, to meet the needs of its Growth and reproduction.Embodiment result shows, the present invention according to microorganism growth to nutraceutical demand, develop a kind of nontoxic and containing biological reinforced dose of bacterial classification, this medicament contains the compositions such as humic acids, vitamin H, amino acid, phytokinin, VITAMIN, trace element, drops in polluted water body, energy accelerate growth of microorganism, make its amount reproduction, activate the activity of indigenous microorganism, by the Co metabolism effect of microorganism, remove the pollutent in water.This biological reinforced dose of raw material is cheap and easy to get, and preparation process is easy, and medicament energy long-time stable is preserved.Adopt this biological reinforced dose of auxiliary process as Industrial Wastewater Treatment, easy and simple to handle, energy-efficient, working cost is low, can be used in all technique utilizing biological method process trade effluent, has broad application prospects.
Claims (6)
1. one kind biological reinforced dose, it is characterized in that, this biological reinforced dose contains peptone, amino acid, glucose, VITAMIN, phytokinin, vitamin H, humic acids, trace element, and the massfraction of each material is:
Peptone 3.0%-5.2%; Amino acid 2.0%-4.5%; Glucose 1.4%-2.7%; VITAMIN 1.2%-2.5%; Phytokinin 0.27%-0.56%; Vitamin H 0.012%-0.037%; Humic acids 0.75%-1.5%; Trace element 0.5-1.5%; Surplus is the sodium chloride aqueous solution of concentration 0.5wt%;
Trace element is inorganic nutrient substance, comprises phosphorus, sulphur, potassium, calcium, magnesium, iron, manganese, copper, cobalt, zinc and molybdenum; In trace element, parts by weight shared are separately respectively phosphorus 10-30 part, sulphur 3-15 part, potassium 5-10 part, calcium 5-10 part, magnesium 1-3 part, iron 0.3-0.5 part, manganese 0.2-0.4 part, copper 0.2-0.5 part, cobalt 0.1-0.3 part, zinc 0.1-0.3 part, molybdenum 0.1-0.2 part.
2. according to biological reinforced dose according to claim 1, it is characterized in that, VITAMIN is one or more of category-B VITAMIN.
3., according to biological reinforced dose according to claim 2, it is characterized in that, category-B VITAMIN be VITMAIN B1, B3, B5 one or more.
4. according to the preparation method of biological reinforced dose according to claim 1, it is characterized in that, component be dissolved in the sodium chloride solution of 0.5 wt %, is light brown liquid by the medicament stirring, leave standstill, filter progress is mixed with, pH value 6.8-7.2.
5. according to the preparation method of biological reinforced dose according to claim 4, it is characterized in that, comprise the steps:
The first step: dissolve; Take peptone, amino acid, glucose, VITAMIN, phytokinin, vitamin H, humic acids, trace element according to described ratio, load weighted material being added respectively concentration is in the sodium chloride solution of 0.5 wt %, makes mixed solution;
Second step: stir; Mixing solutions is slowly stirred 2-4 hour, reaches Homogeneous phase mixing;
3rd step: leave standstill; Solution after stirring at room temperature leaves standstill 4-6 hour;
4th step: filter; Filtered by mixed solution after leaving standstill, the filtrate obtained is biological reinforced dose of preparation.
6. according to the using method of biological reinforced dose according to claim 1, it is characterized in that, by biological reinforced dilution agent 10-20 doubly, add equalizing tank or the water inlet of Sewage treatment systems, the usage quantity of reinforcer is add biological reinforced dose of 10ml-50ml in every cubic metre of sewage.
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| CN102557245A (en) * | 2011-12-26 | 2012-07-11 | 新疆德蓝股份有限公司 | Microbial promotion nutrition agent for improving zoogloea structure and activity of sludge |
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| CN102557245A (en) * | 2011-12-26 | 2012-07-11 | 新疆德蓝股份有限公司 | Microbial promotion nutrition agent for improving zoogloea structure and activity of sludge |
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