CN108911160A - A kind of sewage treatment biological growth promoter and preparation method thereof - Google Patents
A kind of sewage treatment biological growth promoter and preparation method thereof Download PDFInfo
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- CN108911160A CN108911160A CN201810646774.XA CN201810646774A CN108911160A CN 108911160 A CN108911160 A CN 108911160A CN 201810646774 A CN201810646774 A CN 201810646774A CN 108911160 A CN108911160 A CN 108911160A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
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- Organic Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
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Abstract
The invention discloses a kind of sewage treatment biological growth promoters.The sewage treatment is made with biological growth promoter of the raw material of following weight:4~6 parts of glucose, 1~2 part of immobilised enzymes particle, 0.7~1 part of amino acid, 0.1~0.3 part of gibberellin, 0.1~0.3 part of fulvic acid, 0.4~0.6 part of trace-element nutrient, 1~2 part of dihydric phosphate, 1~2 part of ammonium salt.The present invention also provides a kind of preparation methods of sewage treatment biological growth promoter.The present invention provides a kind of sewage treatment biological growth promoter and preparation method thereof, used cost of material is cheap, is easy to buy, product storage time is longer, dosing method is convenient and efficient, saprobia amount can be improved in a short time, it improves system stability and improves sewage treatment capacity, system ecology will not be damaged, and preparation method is simple.
Description
Technical field
The present invention relates to biologic product technology fields, specifically, being related to a kind of sewage treatment biological growth promoter and its system
Preparation Method.
Background technique
With the development of China's industrial or agricultural and being increasing for population, either municipal sewage, trade effluent or agriculture
Schedule has been put in the eutrophic sewage of industry, the technology development in terms of sewage treatment.Sewage quantity increases severely, and sewage is unprocessed just straight
The phenomenon that running in into river is generally existing, and the bearing capacity of surface water body is caused to decline, and pollution problem becomes increasingly conspicuous, serious to restrict
The economic development in city and industry, threatens people's health.
The major progress of water treatment field in recent years and development much concentrate on biofilm treatment process, and biofilm
Treatment process has compared other sewage water treatment methods, has many advantages, such as less investment, technologically advanced, convenient for management, safe and efficient.But
It is during actual use, due to the complicated component of sewage, after adding microbial bacterial agent needed for biofilm, wastewater treatment
System still may face various problems, such as after adding microbial inoculum, and effective bacterium number amount is few, limited activity, the efficiency of processing system
It is lower, to be difficult to quickly to be formed zoogloea, impact resistance weak etc..
Biological growth-promoting preparation is the water process preparation developed rapidly both at home and abroad the 1990s, by environment
Or water treatment system puts into nutrition, accelerates the metabolism of microorganism in water treatment system, improves the COD in sewage treatment industry
The indexs such as removal rate, ammonia nitrogen removal frank, nitrogen removal rate.And grows microbial flora that is original or adding with this, extends life
Object chain makes bioecosystem to high-level succession, increases the community diversity of microorganism, improves the stability of system and support
The ability of imperial external world's undesirable element (such as poisonous substance), to promote Environment-Ecosystem to benign cycle succession.
Currently, the mechanism of action majority of biological growth-promoting preparation technique and Related product is to add nutriment institute group using enzyme
At biological growth promoter is added in waste water treatment system, can effectively facilitate the metabolism of microorganism in waste water, promotes microorganism
Quick flourish in adverse conditions forms the zoogloea with excellent impact resistance energy and degradation property, is mentioned with this
The degradation efficiency of high biofilm.
Summary of the invention
In order to solve the above technical problem, the present invention provides a kind of sewage treatment biological growth promoter and preparation method thereof,
Used cost of material is cheap, is easy to buy, and product storage time is longer, and dosing method is convenient and efficient, can be in the short time
Interior raising saprobia amount improves system stability and improves sewage treatment capacity, will not damage to system ecology, and makes
Preparation Method is simple.
The present invention provides a kind of sewage treatment biological growth promoters, are made of the raw material of following weight:Grape
Sugar 4~6 parts, 1~2 part of immobilised enzymes particle, 0.7~1 part of amino acid, 0.1~0.3 part of gibberellin, 0.1~0.3 part of fulvic acid,
0.4~0.6 part of trace-element nutrient, 1~2 part of dihydric phosphate, 1~2 part of ammonium salt;
The amino acid includes isoleucine, lysine and tryptophan;
The trace-element nutrient includes the mixture of molysite, zinc salt, manganese salt, molybdenum salt.The sewage treatment biology
Growth-promoting agent is made of the raw material of following weight:It is 5 parts of glucose, 1.5 parts of immobilised enzymes particle, 0.8 part of amino acid, red mould
Plain 0.2 part, 0.25 part of fulvic acid, 0.5 part of trace-element nutrient, 1.3 parts of dihydric phosphate, 1.5 parts of ammonium salt.
In a kind of preferred embodiment of sewage treatment biological growth promoter provided by the invention, the immobilised enzymes particle
Preparation method be:
(1) by ferric chloride (FeCl36H2O) and frerrous chloride in molar ratio 1:1~2 ratio is dissolved in the hydrochloric acid of appropriate 0.1mol/L
In solution, hydrochloric acid mixed solution is obtained;
(2) hydrochloric acid mixed solution is put into 0.2mol/L ammonium hydroxide, gained particle is washed to by stirring at normal temperature 30min
PH 7.0, freeze-drying, obtains magnetic ferroferric oxide particle, spare;
(3) the anhydrous second of APTES, 0.63ml water, 150ml of 2.5ml is added by magnetic ferroferric oxide particle described in 0.5g
The ratio of alcohol sequentially adds the magnetic ferroferric oxide particle, APTES, water and dehydrated alcohol, 30 DEG C of stirrings into reactor
10 hours, then product vacuum is freeze-dried, obtains aminofunctional magnetic ferroferric oxide particle;
(4) by horseradish peroxidase and glucose oxidase in molar ratio 2~3:1 mixes, and amino-functional is then added
Change magnetic ferroferric oxide particle and dehydrated alcohol, glutaraldehyde is added after reacting 30min, is finally washed with distillation aquatic products
It washs, obtains immobilised enzymes particle, be put into refrigerator and save backup.
In a kind of preferred embodiment of sewage treatment biological growth promoter provided by the invention, the horseradish peroxidase
The concentration of enzyme is 3mg/mL, and the concentration of the glucose oxidase is 10mg/mL.
In a kind of preferred embodiment of sewage treatment biological growth promoter provided by the invention, the isoleucine, institute
The weight ratio for stating lysine and the tryptophan is 1:0.5~0.8:0.5~0.7.
In a kind of preferred embodiment of sewage treatment biological growth promoter provided by the invention, the molysite, the zinc
The weight ratio of salt, the manganese salt and the molybdenum salt is 1:0.2~0.4:0.1~0.3:0.1~0.3.
The present invention also provides a kind of preparation methods of sewage treatment biological growth promoter, include the following steps:
Step 1: taking glucose, immobilised enzymes particle, amino acid, gibberellin, yellow corruption respectively by the weight
Acid, trace-element nutrient, dihydric phosphate, ammonium salt;
Step 2: respectively by the glucose, the amino acid, the trace-element nutrient, the dihydric phosphate,
The ammonium salt, the gibberellin and the fulvic acid are sequentially added in suitable quantity of water and are dissolved, and are configured to homogeneous solution;
Step 3: the homogeneous solution is uniformly sprayed on the immobilised enzymes particle, be subsequently placed in 30~45 DEG C it is low
Temperature drying is to get sewage treatment biological growth promoter.
In a kind of preferred embodiment of the preparation method of sewage treatment biological growth promoter provided by the invention, the dirt
The water content of water process biological growth promoter is 10~12%.
Compared to the prior art, sewage treatment provided by the invention is had the advantages that with biological growth promoter:
One, sewage treatment of the present invention needs nutriment to be constituted with biological growth promoter by Institute of Micro-biology, includes carbon source, nitrogen
Source, trace-element nutrient etc., bioactivity is high, the ability of resistance BOD loading impact is strong, and biochemical treatment is high-efficient, Ke Yi
Saprobia amount is improved in short time, system stability is improved and simultaneously improves sewage treatment capacity, is suitable for various difficult to degrade or can
The anaerobism or aerobic section of the environment such as industrial wastewater, the black-odor riverway of biochemical difference.
Two, it is by horseradish peroxidase and glucose oxidase with nanoscale magnetic that the present invention, which uses immobilised enzymes particle,
Property ferroso-ferric oxide particle carry out total fixation as crosslinking agent as carrier, glutaraldehyde and be made, horseradish peroxide can be effectively improved
The processing capacity of compound enzyme.
Three, the cost of material of the invention is cheap, is easy to buy, and preparation method is simple and efficient, and prepared biology promotees
Raw agent can storage time it is longer, dosing method is convenient and efficient.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1
In the present embodiment, sewage treatment is made with biological growth promoter of the raw material of following weight:4 parts of glucose,
2 parts of immobilised enzymes particle, 0.7 part of amino acid, 0.1 part of gibberellin, 0.3 part of fulvic acid, 0.4 part of trace-element nutrient, phosphoric acid
2 parts of dihydric salt, 2 parts of ammonium salt;
The preparation method of the immobilised enzymes particle is:
(1) by ferric chloride (FeCl36H2O) and frerrous chloride in molar ratio 1:The hydrochloric acid that 1 ratio is dissolved in appropriate 0.1mol/L is molten
In liquid, hydrochloric acid mixed solution is obtained;
(2) hydrochloric acid mixed solution is put into 0.2mol/L ammonium hydroxide, gained particle is washed to by stirring at normal temperature 30min
PH 7.0, freeze-drying, obtains magnetic ferroferric oxide particle, spare;
(3) the anhydrous second of APTES, 0.63ml water, 150ml of 2.5ml is added by magnetic ferroferric oxide particle described in 0.5g
The ratio of alcohol sequentially adds the magnetic ferroferric oxide particle, APTES, water and dehydrated alcohol, 30 DEG C of stirrings into reactor
10 hours, then product vacuum is freeze-dried, obtains aminofunctional magnetic ferroferric oxide particle;
(4) by horseradish peroxidase and glucose oxidase in molar ratio 2:1 mixes, and aminofunctional magnetic is then added
Property ferroso-ferric oxide particle and dehydrated alcohol, glutaraldehyde is added after reacting 30min, is finally washed, is obtained with distillation aquatic products
Immobilised enzymes particle is put into refrigerator and saves backup.
The amino acid includes isoleucine, lysine and tryptophan, the isoleucine, the lysine and the color
The weight ratio of propylhomoserin is 1:0.5:0.7.
The trace-element nutrient includes the mixture of molysite, zinc salt, manganese salt, molybdenum salt, the molysite, the zinc salt,
The manganese salt and the weight ratio of the molybdenum salt are 1:0.2:0.3:0.3.
The preparation method of the sewage treatment biological growth promoter is as follows:
(1) glucose, immobilised enzymes particle, amino acid, gibberellin, fulvic acid, micro- is taken respectively by the weight
Secondary element nutritional agents, dihydric phosphate, ammonium salt;
(2) respectively by the glucose, amino acid, the trace-element nutrient, the dihydric phosphate, described
Ammonium salt, the gibberellin and the fulvic acid are sequentially added in suitable quantity of water and are dissolved, and are configured to homogeneous solution;
(3) homogeneous solution is uniformly sprayed on the immobilised enzymes particle, is subsequently placed in 30 DEG C of low temperature dryings extremely
Water content is 12% to get sewage treatment biological growth promoter.
Embodiment 2
In the present embodiment, sewage treatment is made with biological growth promoter of the raw material of following weight:5 parts of glucose,
1.5 parts of immobilised enzymes particle, 0.8 part of amino acid, 0.2 part of gibberellin, 0.2 part of fulvic acid, 0.5 part of trace-element nutrient, phosphorus
1.6 parts of acid dihydride salt, 1.8 parts of ammonium salt;
The preparation method of the immobilised enzymes particle is:
(1) by ferric chloride (FeCl36H2O) and frerrous chloride in molar ratio 1:1.5 ratio is dissolved in the hydrochloric acid of appropriate 0.1mol/L
In solution, hydrochloric acid mixed solution is obtained;
(2) hydrochloric acid mixed solution is put into 0.2mol/L ammonium hydroxide, gained particle is washed to by stirring at normal temperature 30min
PH 7.0, freeze-drying, obtains magnetic ferroferric oxide particle, spare;
(3) the anhydrous second of APTES, 0.63ml water, 150ml of 2.5ml is added by magnetic ferroferric oxide particle described in 0.5g
The ratio of alcohol sequentially adds the magnetic ferroferric oxide particle, APTES, water and dehydrated alcohol, 30 DEG C of stirrings into reactor
10 hours, then product vacuum is freeze-dried, obtains aminofunctional magnetic ferroferric oxide particle;
(4) by horseradish peroxidase and glucose oxidase in molar ratio 2.5:1 mixes, and aminofunctional is then added
Magnetic ferroferric oxide particle and dehydrated alcohol are added glutaraldehyde after reacting 30min, are finally washed with distillation aquatic products,
Immobilised enzymes particle is obtained, refrigerator is put into and saves backup.
The amino acid includes isoleucine, lysine and tryptophan, the isoleucine, the lysine and the color
The weight ratio of propylhomoserin is 1:0.7:0.6.
The trace-element nutrient includes the mixture of molysite, zinc salt, manganese salt, molybdenum salt, the molysite, the zinc salt,
The manganese salt and the weight ratio of the molybdenum salt are 1:0.3:0.2:0.2.
The preparation method of the sewage treatment biological growth promoter is as follows:
(1) glucose, immobilised enzymes particle, amino acid, gibberellin, fulvic acid, micro- is taken respectively by the weight
Secondary element nutritional agents, dihydric phosphate, ammonium salt;
(2) respectively by the glucose, amino acid, the trace-element nutrient, the dihydric phosphate, described
Ammonium salt, the gibberellin and the fulvic acid are sequentially added in suitable quantity of water and are dissolved, and are configured to homogeneous solution;
(3) homogeneous solution is uniformly sprayed on the immobilised enzymes particle, is subsequently placed in 40 DEG C of low temperature dryings extremely
Water content is 11% to get sewage treatment biological growth promoter.
Embodiment 3
In the present embodiment, sewage treatment is made with biological growth promoter of the raw material of following weight:6 parts of glucose,
1 part of immobilised enzymes particle, amino acid 1 part, 0.3 part of gibberellin, 0.1 part of fulvic acid, 0.6 part of trace-element nutrient, di(2-ethylhexyl)phosphate
1 part of hydrogen salt, 1 part of ammonium salt;
The preparation method of the immobilised enzymes particle is:
(1) by ferric chloride (FeCl36H2O) and frerrous chloride in molar ratio 1:The hydrochloric acid that 2 ratio is dissolved in appropriate 0.1mol/L is molten
In liquid, hydrochloric acid mixed solution is obtained;
(2) hydrochloric acid mixed solution is put into 0.2mol/L ammonium hydroxide, gained particle is washed to by stirring at normal temperature 30min
PH 7.0, freeze-drying, obtains magnetic ferroferric oxide particle, spare;
(3) the anhydrous second of APTES, 0.63ml water, 150ml of 2.5ml is added by magnetic ferroferric oxide particle described in 0.5g
The ratio of alcohol sequentially adds the magnetic ferroferric oxide particle, APTES, water and dehydrated alcohol, 30 DEG C of stirrings into reactor
10 hours, then product vacuum is freeze-dried, obtains aminofunctional magnetic ferroferric oxide particle;
(4) by horseradish peroxidase and glucose oxidase in molar ratio 3:1 mixes, and aminofunctional magnetic is then added
Property ferroso-ferric oxide particle and dehydrated alcohol, glutaraldehyde is added after reacting 30min, is finally washed, is obtained with distillation aquatic products
Immobilised enzymes particle is put into refrigerator and saves backup.
The amino acid includes isoleucine, lysine and tryptophan, the isoleucine, the lysine and the color
The weight ratio of propylhomoserin is 1:0.8:0.5.
The trace-element nutrient includes the mixture of molysite, zinc salt, manganese salt, molybdenum salt, the molysite, the zinc salt,
The manganese salt and the weight ratio of the molybdenum salt are 1:0.4:0.1:0.1.
The preparation method of the sewage treatment biological growth promoter is as follows:
(1) glucose, immobilised enzymes particle, amino acid, gibberellin, fulvic acid, micro- is taken respectively by the weight
Secondary element nutritional agents, dihydric phosphate, ammonium salt;
(2) respectively by the glucose, amino acid, the trace-element nutrient, the dihydric phosphate, described
Ammonium salt, the gibberellin and the fulvic acid are sequentially added in suitable quantity of water and are dissolved, and are configured to homogeneous solution;
(3) homogeneous solution is uniformly sprayed on the immobilised enzymes particle, is subsequently placed in 45 DEG C of low temperature dryings extremely
Water content is 10% to get sewage treatment biological growth promoter.
Sewage treatment provided by the invention is had the advantages that with biological growth promoter:
One, sewage treatment of the present invention needs nutriment to be constituted with biological growth promoter by Institute of Micro-biology, includes carbon source, nitrogen
Source, trace-element nutrient etc., bioactivity is high, the ability of resistance BOD loading impact is strong, and biochemical treatment is high-efficient, Ke Yi
Saprobia amount is improved in short time, system stability is improved and simultaneously improves sewage treatment capacity, is suitable for various difficult to degrade or can
The anaerobism or aerobic section of the environment such as industrial wastewater, the black-odor riverway of biochemical difference.
Two, it is by horseradish peroxidase and glucose oxidase with nanoscale magnetic that the present invention, which uses immobilised enzymes particle,
Property ferroso-ferric oxide particle carry out total fixation as crosslinking agent as carrier, glutaraldehyde and be made, horseradish peroxide can be effectively improved
The processing capacity of compound enzyme.
Three, the cost of material of the invention is cheap, is easy to buy, and preparation method is simple and efficient, and prepared biology promotees
Raw agent can storage time it is longer, dosing method is convenient and efficient.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (8)
1. a kind of sewage treatment biological growth promoter, which is characterized in that be made of the raw material of following weight:Glucose 4
~6 parts, it is 1~2 part of immobilised enzymes particle, 0.7~1 part of amino acid, 0.1~0.3 part of gibberellin, 0.1~0.3 part of fulvic acid, micro-
0.4~0.6 part of secondary element nutritional agents, 1~2 part of dihydric phosphate, 1~2 part of ammonium salt;The amino acid includes isoleucine, relies
Propylhomoserin and tryptophan;The trace-element nutrient includes the mixture of molysite, zinc salt, manganese salt, molybdenum salt.
2. sewage treatment biological growth promoter according to claim 1, which is characterized in that the sewage treatment is promoted with biology
Raw agent is made of the raw material of following weight:5 parts of glucose, 1.5 parts of immobilised enzymes particle, 0.8 part of amino acid, gibberellin
0.2 part, 0.25 part of fulvic acid, 0.5 part of trace-element nutrient, 1.3 parts of dihydric phosphate, 1.5 parts of ammonium salt.
3. sewage treatment biological growth promoter according to claim 1, which is characterized in that the system of the immobilised enzymes particle
Preparation Method is:
(1) by ferric chloride (FeCl36H2O) and frerrous chloride in molar ratio 1:1~2 ratio is dissolved in the hydrochloric acid solution of appropriate 0.1mol/L
In, obtain hydrochloric acid mixed solution;
(2) hydrochloric acid mixed solution is put into 0.2mol/L ammonium hydroxide, stirring at normal temperature 30min, gained particle is washed to pH
7.0, freeze-drying obtains magnetic ferroferric oxide particle, spare;
(3) APTES, 0.63ml water of 2.5ml, 150ml dehydrated alcohol is added by magnetic ferroferric oxide particle described in 0.5g
Ratio, the magnetic ferroferric oxide particle, APTES, water and dehydrated alcohol are sequentially added into reactor, and 30 DEG C of stirrings 10 are small
When, then product vacuum is freeze-dried, obtains aminofunctional magnetic ferroferric oxide particle;
(4) by horseradish peroxidase and glucose oxidase in molar ratio 2~3:1 mixes, and aminofunctional magnetic is then added
Property ferroso-ferric oxide particle and dehydrated alcohol, glutaraldehyde is added after reacting 30min, is finally washed, is obtained with distillation aquatic products
Immobilised enzymes particle is put into refrigerator and saves backup.
4. sewage treatment biological growth promoter according to claim 3, which is characterized in that the horseradish peroxidase
Concentration is 3mg/mL, and the concentration of the glucose oxidase is 10mg/mL.
5. sewage treatment biological growth promoter according to claim 1, which is characterized in that the isoleucine, it is described rely
The weight ratio of propylhomoserin and the tryptophan is 1:0.5~0.8:0.5~0.7.
6. sewage treatment biological growth promoter according to claim 1, which is characterized in that the molysite, the zinc salt, institute
The weight ratio for stating manganese salt and the molybdenum salt is 1:0.2~0.4:0.1~0.3:0.1~0.3.
7. a kind of preparation method based on any sewage treatment biological growth promoter of claim 1~6, feature exist
In including the following steps:
Step 1: taking glucose, immobilised enzymes particle, amino acid, gibberellin, fulvic acid, micro- respectively by the weight
Secondary element nutritional agents, dihydric phosphate, ammonium salt;
Step 2: respectively by the glucose, amino acid, the trace-element nutrient, the dihydric phosphate, described
Ammonium salt, the gibberellin and the fulvic acid are sequentially added in suitable quantity of water and are dissolved, and are configured to homogeneous solution;
Step 3: the homogeneous solution is uniformly sprayed on the immobilised enzymes particle, it is subsequently placed in 30~45 DEG C of low temperature and dries
It does to get sewage treatment biological growth promoter.
8. the preparation method of sewage treatment biological growth promoter according to claim 7, which is characterized in that at the sewage
The water content of reason biological growth promoter is 10~12%.
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CN109970213A (en) * | 2019-04-03 | 2019-07-05 | 石家庄宝川生物科技有限公司 | Biological bacteria conditioner |
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