CN105462903A - Efficient sewage treatment microbial agent - Google Patents

Efficient sewage treatment microbial agent Download PDF

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Publication number
CN105462903A
CN105462903A CN201610032517.8A CN201610032517A CN105462903A CN 105462903 A CN105462903 A CN 105462903A CN 201610032517 A CN201610032517 A CN 201610032517A CN 105462903 A CN105462903 A CN 105462903A
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sewage treatment
treatment microbial
efficient sewage
microbial inoculant
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张文荟
钟诚
吴庆儒
姜蒙
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NANJING GLOBAL BIOLOGICAL SCIENCE & TECHNOLOGY Co Ltd
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NANJING GLOBAL BIOLOGICAL SCIENCE & TECHNOLOGY Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/347Use of yeasts or fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast

Abstract

The invention discloses an efficient sewage treatment microbial agent. The efficient sewage treatment microbial agent is prepared from, by weight, 2-4 parts of nitrosobacteria, 10-20 parts of bacillus subtilis, 8-15 parts of alcaligenes faecalis, 5-10 parts of bacillus megatherium, 2-8 parts of pseudomonas aeruginosa, 2-10 parts of candida lipolytica, 2-8 parts of deoxidized paracoccus, 2-6 parts of pseudomonas stutzeri, 2-10 parts of saccharomyces cerevisiae, 1-4 parts of aspergillus niger, 0.1-0.6 part of yeast extract, 2-6 parts of peptone, 0.1-0.5 part of glucose, 8-15 parts of kaolin, 2-5 parts of aluminum polychlorid, 0.1-0.5 part of FMEE and a proper amount of water. The efficient sewage treatment microbial agent is suitable for treating industrial waste water, breeding waste water and the like, all strains are in cooperation, symbiotic coordination is achieved, high activity is achieved, and reproduction is fast. The efficient sewage treatment microbial agent is put in the waste water to be treated, the degradation effect can be effectively accelerated, the treatment water quality can be greatly improved, operating cost can be reduced, the treated water reaches the standard to be drained, and damage to environment is reduced.

Description

A kind of sewage treatment microbial inoculant efficiently
Technical field
The present invention relates to technical field of water treatment chemical, particularly relate to a kind of sewage treatment microbial inoculant efficiently.
Background technology
At present, industry, incomparable effect is played in the process of cultivation and sanitary wastewater in protection of the environment, but inside sewage treatment area, still there is a lot of problem, such as industrial sewage is unstable when water quality treatment effect, and operation cost is higher, water after purification needs through passing into the disinfections such as chlorine, causing is the microorganism generation mortality carrying the inside originally, thus allow water body lose the ability of self-purification originally, there is higher phenomenon in the chemical composition in sewage-treating agent, easily secondary pollution etc. is caused to water body, the COD of aquaculture wastewater and ammonia nitrogen superelevation, traditional biogas and active sludge cannot reach the requirement of more and more tighter environmental protection index, and produce a large amount of mud, difficult treatment.And for sewage disposal, simple passes through microbiological deterioration, and speed is slow, is not easy to take effect very soon.For this reason, we propose a kind of sewage treatment microbial inoculant efficiently.
Summary of the invention
Based on the technical problem that background technology exists, the present invention proposes a kind of sewage treatment microbial inoculant efficiently.
The present invention proposes a kind of sewage treatment microbial inoculant efficiently, its raw material comprises by weight: nitrococcus 2-4 part, subtilis 10-20 part, Bacillus foecalis alkaligenes 8-15 part, bacillus megaterium 5-10 part, Pseudomonas aeruginosa 2-8 part, Candida lipolytica 2-10 part, deoxidation secondary coccus 2-8 part, Pseudomonas stutzeri 2-6 part, yeast saccharomyces cerevisiae 2-10 part, aspergillus niger 1-4 part, yeast extract paste 0.1-0.6 part, peptone 2-6 part, glucose 0.1-0.5 part, kaolin 8-15 part, polymerize aluminum chloride 2-5 part, aliphatic acid polyethenoxy methyl ether 0.1-0.5 part, suitable quantity of water.
Preferably, the weight ratio of subtilis, Bacillus foecalis alkaligenes, bacillus megaterium is 15-17:11-13:6-8.
Preferably, the weight ratio of Pseudomonas aeruginosa, Candida lipolytica, the secondary coccus of deoxidation, Pseudomonas stutzeri, yeast saccharomyces cerevisiae is 5-7:6-8:5-7:3-4:6-8.
Preferably, aspergillus niger, yeast extract paste, peptone, the weight ratio of glucose is 2-3:0.3-0.5:3-5:0.3-0.5.
Preferably, the efficient sewage treatment microbial inoculant of described one, its raw material comprises by weight: nitrococcus 2.5-3.2 part, subtilis 17-19 part, Bacillus foecalis alkaligenes 10-13 part, bacillus megaterium 6-8 part, Pseudomonas aeruginosa 3-6 part, Candida lipolytica 5-8 part, deoxidation secondary coccus 5-6 part, Pseudomonas stutzeri 4-5 part, yeast saccharomyces cerevisiae 5-7 part, aspergillus niger 2.5-3.5 part, yeast extract paste 0.3-0.5 part, peptone 3.5-5 part, glucose 0.3-0.45 part, kaolin 10-12 part, polymerize aluminum chloride 3-4 part, aliphatic acid polyethenoxy methyl ether 0.3-0.4 part.
Preferably, the efficient sewage treatment microbial inoculant of described one, its raw material comprises by weight: nitrococcus 3 parts, subtilis 16 parts, Bacillus foecalis alkaligenes 12 parts, bacillus megaterium 7 parts, Pseudomonas aeruginosa 5.5 parts, Candida lipolytica 7.5 parts, secondary 6 parts, the coccus of deoxidation, Pseudomonas stutzeri 5 parts, yeast saccharomyces cerevisiae 6.5 parts, aspergillus niger 3 parts, yeast extract paste 0.35 part, peptone 4.5 parts, glucose 0.4 part, kaolin 11 parts, polymerize aluminum chloride 3.5 parts, aliphatic acid polyethenoxy methyl ether 0.35 part.
Disclosed in the present invention also, a kind of preparation method of efficient sewage treatment microbial inoculant, comprises the steps:
S1, provide mixed strains substratum, this mixed strains substratum is put in this mixed strains substratum the peptone, glucose, yeast extract paste and the water that take according to deal proportioning through high-temperature sterilization process;
S2, to take nitrococcus, subtilis, Bacillus foecalis alkaligenes, bacillus megaterium, Pseudomonas aeruginosa, Candida lipolytica, the secondary coccus of deoxidation, Pseudomonas stutzeri, yeast saccharomyces cerevisiae, aspergillus niger pour stirrer into and carry out stirring 5-10 minute and obtain mixed strains according to deal proportioning;
S3, to be placed in mixed strains substratum by gained mixed strains in S2 and to cultivate, culture temperature is 25-35 DEG C, and incubation time is 30-45 hour, can obtain complex microorganism after incubation time;
S4, by complex microorganism through centrifugal drying process, kaolin, polymerize aluminum chloride and aliphatic acid polyethenoxy methyl ether is taken again according to deal proportioning, and be placed in stirrer to stir and obtain mixture, then by mixture with dried complex microorganism heated and stirred machine is stirred just can obtain efficient sewage treatment microbial inoculant.
In the present invention, each bacterial classification wherein in nitrococcus, subtilis, Bacillus foecalis alkaligenes, bacillus megaterium, Pseudomonas aeruginosa, Candida lipolytica, the secondary coccus of deoxidation, Pseudomonas stutzeri, yeast saccharomyces cerevisiae can accelerate the degraded of microorganism to sewage, to improve the bioavailability of whole Waste Water Treatment, thus accelerate the operation at waste water place, the foreign material accelerated in sewage decompose, cooperatively interact between each bacterial classification, symbiosis is coordinated, active high, and breeding is fast, be dropped in waste water to be dealt with, effectively can be accelerated degradation effect; Multiple harmful ion in the kaolin energy planar water added, further by sewage purification, it is wide that the polymerize aluminum chloride wherein added adapts to waters, and hydrolysis rate is fast, high adsorption capacity, forms alumen ustum large, and the close precipitation of matter is fast, the advantages such as delivery turbidity is low, and dewatering is good; The aliphatic acid polyethenoxy methyl ether added has fast, strong to the grease solubilising feature of cheap, the low bubble of raw material, water-soluble speed.The present invention is applicable to the process of the waste water such as industry and cultivation, can improve water quality treatment in a large number, reduces running cost, allows and processes water qualified discharge, reduce the destruction to environment.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
A kind of sewage treatment microbial inoculant efficiently, its raw material comprises by weight: nitrococcus 2 parts, subtilis 11 parts, Bacillus foecalis alkaligenes 8.5 parts, bacillus megaterium 5.5 parts, Pseudomonas aeruginosa 3 parts, Candida lipolytica 3 parts, secondary 2 parts, the coccus of deoxidation, Pseudomonas stutzeri 2 parts, yeast saccharomyces cerevisiae 3 parts, aspergillus niger 2 parts, yeast extract paste 0.15 part, peptone 2 parts, glucose 0.15 part, kaolin 8 parts, polymerize aluminum chloride 2 parts, aliphatic acid polyethenoxy methyl ether 0.1 part, suitable quantity of water.
Its preparation method comprises the following steps:
S1, provide mixed strains substratum, this mixed strains substratum is put in this mixed strains substratum the peptone, glucose, yeast extract paste and the water that take according to deal proportioning through high-temperature sterilization process;
S2, to take nitrococcus, subtilis, Bacillus foecalis alkaligenes, bacillus megaterium, Pseudomonas aeruginosa, Candida lipolytica, the secondary coccus of deoxidation, Pseudomonas stutzeri, yeast saccharomyces cerevisiae, aspergillus niger pour stirrer into and carry out stirring and obtain mixed strains in 5 minutes according to deal proportioning;
S3, to be placed in mixed strains substratum by gained mixed strains in S2 and to cultivate, culture temperature is 26 DEG C, and incubation time is 30 hours, can obtain complex microorganism after incubation time;
S4, by complex microorganism through centrifugal drying process, kaolin, polymerize aluminum chloride and aliphatic acid polyethenoxy methyl ether is taken again according to deal proportioning, and be placed in stirrer to stir and obtain mixture, then by mixture with dried complex microorganism heated and stirred machine is stirred just can obtain efficient sewage treatment microbial inoculant.
Embodiment 2
A kind of sewage treatment microbial inoculant efficiently, its raw material comprises by weight: nitrococcus 2.5 parts, subtilis 14 parts, Bacillus foecalis alkaligenes 10 parts, bacillus megaterium 6.5 parts, Pseudomonas aeruginosa 4 parts, Candida lipolytica 5 parts, secondary 4.5 parts, the coccus of deoxidation, Pseudomonas stutzeri 3.5 parts, yeast saccharomyces cerevisiae 4.5 parts, aspergillus niger 3.2 parts, yeast extract paste 0.35 part, peptone 3 parts, glucose 0.25 part, kaolin 10 parts, polymerize aluminum chloride 2.8 parts, aliphatic acid polyethenoxy methyl ether 0.25 part, suitable quantity of water.
Its preparation method comprises the following steps:
S1, provide mixed strains substratum, this mixed strains substratum is put in this mixed strains substratum the peptone, glucose, yeast extract paste and the water that take according to deal proportioning through high-temperature sterilization process;
S2, to take nitrococcus, subtilis, Bacillus foecalis alkaligenes, bacillus megaterium, Pseudomonas aeruginosa, Candida lipolytica, the secondary coccus of deoxidation, Pseudomonas stutzeri, yeast saccharomyces cerevisiae, aspergillus niger pour stirrer into and carry out stirring and obtain mixed strains in 6 minutes according to deal proportioning;
S3, to be placed in mixed strains substratum by gained mixed strains in S2 and to cultivate, culture temperature is 28 DEG C, and incubation time is 35 hours, can obtain complex microorganism after incubation time;
S4, by complex microorganism through centrifugal drying process, kaolin, polymerize aluminum chloride and aliphatic acid polyethenoxy methyl ether is taken again according to deal proportioning, and be placed in stirrer to stir and obtain mixture, then by mixture with dried complex microorganism heated and stirred machine is stirred just can obtain efficient sewage treatment microbial inoculant.
Embodiment 3
A kind of sewage treatment microbial inoculant efficiently, its raw material comprises by weight: nitrococcus 3 parts, subtilis 16 parts, Bacillus foecalis alkaligenes 12 parts, bacillus megaterium 7 parts, Pseudomonas aeruginosa 5.5 parts, Candida lipolytica 7.5 parts, secondary 6 parts, the coccus of deoxidation, Pseudomonas stutzeri 5 parts, yeast saccharomyces cerevisiae 6.5 parts, aspergillus niger 3 parts, yeast extract paste 0.35 part, peptone 4.5 parts, glucose 0.4 part, kaolin 11 parts, polymerize aluminum chloride 3.5 parts, aliphatic acid polyethenoxy methyl ether 0.35 part, suitable quantity of water.
Its preparation method comprises the following steps:
S1, provide mixed strains substratum, this mixed strains substratum is put in this mixed strains substratum the peptone, glucose, yeast extract paste and the water that take according to deal proportioning through high-temperature sterilization process;
S2, to take nitrococcus, subtilis, Bacillus foecalis alkaligenes, bacillus megaterium, Pseudomonas aeruginosa, Candida lipolytica, the secondary coccus of deoxidation, Pseudomonas stutzeri, yeast saccharomyces cerevisiae, aspergillus niger pour stirrer into and carry out stirring and obtain mixed strains in 7 minutes according to deal proportioning;
S3, to be placed in mixed strains substratum by gained mixed strains in S2 and to cultivate, culture temperature is 30 DEG C, and incubation time is 38 hours, can obtain complex microorganism after incubation time;
S4, by complex microorganism through centrifugal drying process, kaolin, polymerize aluminum chloride and aliphatic acid polyethenoxy methyl ether is taken again according to deal proportioning, and be placed in stirrer to stir and obtain mixture, then by mixture with dried complex microorganism heated and stirred machine is stirred just can obtain efficient sewage treatment microbial inoculant.
Embodiment 4
A kind of sewage treatment microbial inoculant efficiently, its raw material comprises by weight: nitrococcus 4 parts, subtilis 18 parts, Bacillus foecalis alkaligenes 14 parts, bacillus megaterium 9 parts, Pseudomonas aeruginosa 7.5 parts, Candida lipolytica 8.5 parts, secondary 7.5 parts, the coccus of deoxidation, Pseudomonas stutzeri 5.5 parts, yeast saccharomyces cerevisiae 9 parts, aspergillus niger 3.5 parts, yeast extract paste 0.5 part, peptone 5 parts, glucose 0.45 part, kaolin 14 parts, polymerize aluminum chloride 4.5 parts, aliphatic acid polyethenoxy methyl ether 0.45 part, suitable quantity of water.
Its preparation method comprises the following steps:
S1, provide mixed strains substratum, this mixed strains substratum is put in this mixed strains substratum the peptone, glucose, yeast extract paste and the water that take according to deal proportioning through high-temperature sterilization process;
S2, to take nitrococcus, subtilis, Bacillus foecalis alkaligenes, bacillus megaterium, Pseudomonas aeruginosa, Candida lipolytica, the secondary coccus of deoxidation, Pseudomonas stutzeri, yeast saccharomyces cerevisiae, aspergillus niger pour stirrer into and carry out stirring and obtain mixed strains in 9 minutes according to deal proportioning;
S3, to be placed in mixed strains substratum by gained mixed strains in S2 and to cultivate, culture temperature is 33 DEG C, and incubation time is 43 hours, can obtain complex microorganism after incubation time;
S4, by complex microorganism through centrifugal drying process, kaolin, polymerize aluminum chloride and aliphatic acid polyethenoxy methyl ether is taken again according to deal proportioning, and be placed in stirrer to stir and obtain mixture, then by mixture with dried complex microorganism heated and stirred machine is stirred just can obtain efficient sewage treatment microbial inoculant.
The above; be only the present invention's preferably embodiment; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.

Claims (7)

1. an efficient sewage treatment microbial inoculant, it is characterized in that, its raw material comprises by weight: nitrococcus 2-4 part, subtilis 10-20 part, Bacillus foecalis alkaligenes 8-15 part, bacillus megaterium 5-10 part, Pseudomonas aeruginosa 2-8 part, Candida lipolytica 2-10 part, deoxidation secondary coccus 2-8 part, Pseudomonas stutzeri 2-6 part, yeast saccharomyces cerevisiae 2-10 part, aspergillus niger 1-4 part, yeast extract paste 0.1-0.6 part, peptone 2-6 part, glucose 0.1-0.5 part, kaolin 8-15 part, polymerize aluminum chloride 2-5 part, aliphatic acid polyethenoxy methyl ether 0.1-0.5 part, suitable quantity of water.
2. the efficient sewage treatment microbial inoculant of one according to claim 1, is characterized in that, the weight ratio of subtilis, Bacillus foecalis alkaligenes, bacillus megaterium is 15-17:11-13:6-8.
3. the efficient sewage treatment microbial inoculant of one according to claim 1, is characterized in that, the weight ratio of Pseudomonas aeruginosa, Candida lipolytica, the secondary coccus of deoxidation, Pseudomonas stutzeri, yeast saccharomyces cerevisiae is 5-7:6-8:5-7:3-4:6-8.
4. the efficient sewage treatment microbial inoculant of one according to claim 1, is characterized in that, aspergillus niger, yeast extract paste, peptone, and the weight ratio of glucose is 2-3:0.3-0.5:3-5:0.3-0.5.
5. the efficient sewage treatment microbial inoculant of one according to claim 1, it is characterized in that, its raw material comprises by weight: nitrococcus 2.5-3.2 part, subtilis 17-19 part, Bacillus foecalis alkaligenes 10-13 part, bacillus megaterium 6-8 part, Pseudomonas aeruginosa 3-6 part, Candida lipolytica 5-8 part, deoxidation secondary coccus 5-6 part, Pseudomonas stutzeri 4-5 part, yeast saccharomyces cerevisiae 5-7 part, aspergillus niger 2.5-3.5 part, yeast extract paste 0.3-0.5 part, peptone 3.5-5 part, glucose 0.3-0.45 part, kaolin 10-12 part, polymerize aluminum chloride 3-4 part, aliphatic acid polyethenoxy methyl ether 0.3-0.4 part.
6. a kind of sewage treatment microbial inoculant efficiently according to claim 1 or 5, it is characterized in that, its raw material comprises by weight: nitrococcus 3 parts, subtilis 16 parts, Bacillus foecalis alkaligenes 12 parts, bacillus megaterium 7 parts, Pseudomonas aeruginosa 5.5 parts, Candida lipolytica 7.5 parts, secondary 6 parts, the coccus of deoxidation, Pseudomonas stutzeri 5 parts, yeast saccharomyces cerevisiae 6.5 parts, aspergillus niger 3 parts, yeast extract paste 0.35 part, peptone 4.5 parts, glucose 0.4 part, kaolin 11 parts, polymerize aluminum chloride 3.5 parts, aliphatic acid polyethenoxy methyl ether 0.35 part.
7. a preparation method for a kind of efficient sewage treatment microbial inoculant according to any one of claim 1-6, is characterized in that, comprise the steps:
S1, provide mixed strains substratum, this mixed strains substratum is put in this mixed strains substratum the peptone, glucose, yeast extract paste and the water that take according to deal proportioning through high-temperature sterilization process;
S2, to take nitrococcus, subtilis, Bacillus foecalis alkaligenes, bacillus megaterium, Pseudomonas aeruginosa, Candida lipolytica, the secondary coccus of deoxidation, Pseudomonas stutzeri, yeast saccharomyces cerevisiae, aspergillus niger pour stirrer into and carry out stirring 5-10 minute and obtain mixed strains according to deal proportioning;
S3, to be placed in mixed strains substratum by gained mixed strains in S2 and to cultivate, culture temperature is 25-35 DEG C, and incubation time is 30-45 hour, can obtain complex microorganism after incubation time;
S4, by complex microorganism through centrifugal drying process, kaolin, polymerize aluminum chloride and aliphatic acid polyethenoxy methyl ether is taken again according to deal proportioning, and be placed in stirrer to stir and obtain mixture, then by mixture with dried complex microorganism heated and stirred machine is stirred just can obtain efficient sewage treatment microbial inoculant.
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CN105907673B (en) * 2016-04-29 2018-02-06 广州市佳境水处理技术工程有限公司 A kind of method using microorganism formulation enhanced water treatment biochemical effects
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