CN113754076A - Preparation method of microbial nutrient for biochemical treatment of wastewater - Google Patents
Preparation method of microbial nutrient for biochemical treatment of wastewater Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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Abstract
The invention discloses a preparation method of a microbial nutrient for wastewater biochemical treatment, which comprises the following steps: s1, cleaning the wheat roots, placing the wheat roots into a drying box for drying, crushing the wheat roots after drying, and sieving the wheat roots with a 50-80-mesh sieve; s2, adding the screened wheat roots into an ultrasonic extractor, adding 20-30 times of water, performing ultrasonic extraction for 1-3 times, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure to obtain a wheat root concentrated solution, and performing spray drying to obtain a wheat root extract; s3, placing the wheat root extract in a supercritical extraction kettle for supercritical carbon dioxide extraction to obtain an extract; s4, mixing and stirring the potassium fulvate, the ammonium molybdate tetrahydrate, the ferrous sulfate heptahydrate and the distilled water for 20-30min, controlling the temperature at 30-50 ℃, wherein the nutrient prepared by the preparation method of the microbial nutrient for biochemical treatment of wastewater has a remarkable effect of improving the activity of microorganisms, so that the input amount can be reduced, the raw materials are low in price and easy to obtain.
Description
Technical Field
The invention relates to the technical field of microbial nutrients, in particular to a preparation method of a microbial nutrient for biochemical treatment of wastewater.
Background
The waste water is a general term of water and runoff rainwater drained in the process of activities of residents. The method comprises the steps that domestic sewage, industrial wastewater and initial rainwater flow into a drainage pipe canal and other useless water, generally, the water cannot be recycled after certain technical treatment or the water with certain standard-unavailable purification treatment difficulty is prepared after primary pollution, the industrial wastewater directly flows into channels, rivers and lakes to pollute surface water, if the toxicity is high, aquatic animals and plants die and even disappear, and the industrial wastewater can also permeate into underground water to pollute the underground water; if the polluted surface water or underground water is used as domestic water by surrounding residents, the health of the residents can be harmed, and serious residents die; the industrial wastewater permeates into the soil, causing soil pollution. Influences the growth of microorganisms in plants and soil, some industrial wastewater has unpleasant odor and pollutes air, toxic and harmful substances in the industrial wastewater can remain in the body under the feeding and absorption actions of passive plants and then reach the human body through a food chain, the method mainly comprises a physical method, a chemical method and a biological method, wherein the biological method is used for creating an environment which is beneficial to the growth and the reproduction of microorganisms so as to ensure that the microorganisms multiply in quantity, so as to improve the oxidation of microorganisms, decompose organic pollutants, degrade and convert the organic pollutants into harmless substances, purify sewage, and have a plurality of advantages in application, if the comprehensive treatment capacity is strong, harmful metal ions such as copper, hexavalent chromium, nickel, zinc, chromium, lead and the like in the wastewater are effectively removed; the treatment method is simple and practical; the process control is simple; the sludge amount is less, the secondary pollution is obviously reduced, because of the reasons of high wastewater pollution concentration, high biological toxicity and the like, the activity of microorganisms in wastewater is poor, and the treatment effect is influenced, so people can add biological nutrient to the wastewater for improving the activity of the microorganisms, but the nutrient components provided by the existing biological nutrient are single, so that the types of the microorganisms which can be influenced to improve the activity are less, the effect of the biological nutrient is poor, and the use requirements of people cannot be met.
Disclosure of Invention
The invention aims to provide a preparation method of a microbial nutrient for biochemical treatment of wastewater, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of a microbial nutrient for wastewater biochemical treatment comprises the following steps:
s1, cleaning the wheat roots, placing the wheat roots into a drying box for drying, crushing the wheat roots after drying, and sieving the wheat roots with a 50-80-mesh sieve;
s2, adding the screened wheat roots into an ultrasonic extractor, adding 20-30 times of water, performing ultrasonic extraction for 1-3 times, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure to obtain a wheat root concentrated solution, and performing spray drying to obtain a wheat root extract;
s3, placing the wheat root extract in a supercritical extraction kettle for supercritical carbon dioxide extraction to obtain an extract;
s4, mixing and stirring potassium fulvate, ammonium molybdate tetrahydrate, ferrous sulfate heptahydrate and distilled water for 20-30min, controlling the temperature at 30-50 ℃, adding into a centrifuge, centrifuging, and taking supernatant;
s5, adding the extract, the supernatant, cobalt chloride hexahydrate, sodium o-nitrophenolate, hemin and bioactive phosphorus into a stirring kettle, controlling the temperature of the stirring kettle to be kept at 50-70 ℃, stirring for 30-60min, standing and cooling to obtain the microbial nutrient for the biochemical treatment of wastewater.
In a preferred embodiment of the present invention, the weight ratio of the wheat root, the distilled water, the ammonium molybdate tetrahydrate, the ferrous sulfate heptahydrate, the potassium fulvate, the cobalt chloride hexahydrate, the sodium o-nitrophenolate, the hemin and the bioactive phosphorus is 33-55%, 10-12% of distilled water, 4-6% of ammonium molybdate tetrahydrate, 1-2% of ferrous sulfate heptahydrate, 20-30% of potassium fulvate, 3-5% of cobalt chloride hexahydrate, 1.5-3% of sodium o-nitrophenolate, 0.5-2% of hemin and 5-7% of bioactive phosphorus.
As a preferable scheme of the present invention, the drying oven in step S1 is a blowing-type drying oven, the drying temperature is 60-90 ℃, and the drying time is 1-2 h.
As a preferable scheme of the invention, each ultrasonic extraction time in the step S2 is 1-3h, the inlet temperature in the spray drying device is 100-200 ℃ and the outlet temperature is 30-60 ℃.
As a preferable scheme of the invention, the supercritical carbon dioxide extraction pressure in the step S3 is 13-22Mpa, the carbon dioxide flow rate is 10-15kg/h, the extraction temperature is 40-60 ℃, and the extraction time is 15-35 min.
In a preferred embodiment of the present invention, in the step S4, the stirring speed is 60-120r/min, the centrifugal force is 80000-100000Xg, the centrifugation time is 10-25min, and the centrifugation temperature is 10-30 ℃.
As a preferable scheme of the invention, the stirring speed in the step S5 is 100-165r/min, and the cooling time is 3-5 h.
Compared with the prior art, the invention has the beneficial effects that: the nutrient prepared by the preparation method of the microbial nutrient for biochemical treatment of wastewater has obvious effect on improving the activity of microorganisms, thereby reducing the input amount, having low price of raw materials, easy acquisition and reduced production and use cost, can provide K, P, Fe and other elements for the microorganisms by adding potassium fulvate, ammonium molybdate tetrahydrate, bioactive phosphorus and ferrous sulfate heptahydrate, can provide an organic carbon source for the microorganisms by adding wheat roots, contains a small amount of sugar, fat, growth factors and the like besides rich protein, peptide and free amino acid, can play a good role in improving the activity for different types of microorganisms by mutually matching the components, and can provide a necessary cobalt element for the growth of the microorganisms by adding cobalt chloride hexahydrate, the microbial synthesis of vitamin B12 requires the participation of cobalt, the cobalt is beneficial to the synthesis of microbial nitrogen, the added hemin can be used as a growth stimulating factor of the microorganism, so that the microorganism can grow better, the establishment of a co-metabolic system is accelerated, the metabolism of the microorganism to target pollutants is enhanced, the protoplasm flow of the microorganism can be promoted through the added sodium o-nitrophenolate, and the microbial activity is improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: a preparation method of a microbial nutrient for wastewater biochemical treatment comprises the following steps:
s1, cleaning the wheat roots, placing the wheat roots into a drying box for drying, crushing the wheat roots after drying, and sieving the wheat roots with a 50-80-mesh sieve;
s2, adding the screened wheat roots into an ultrasonic extractor, adding 20-30 times of water, performing ultrasonic extraction for 1-3 times, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure to obtain a wheat root concentrated solution, and performing spray drying to obtain a wheat root extract;
s3, placing the wheat root extract in a supercritical extraction kettle for supercritical carbon dioxide extraction to obtain an extract;
s4, mixing and stirring potassium fulvate, ammonium molybdate tetrahydrate, ferrous sulfate heptahydrate and distilled water for 20-30min, controlling the temperature at 30-50 ℃, adding into a centrifuge, centrifuging, and taking supernatant;
s5, adding the extract, the supernatant, cobalt chloride hexahydrate, sodium o-nitrophenolate, hemin and bioactive phosphorus into a stirring kettle, controlling the temperature of the stirring kettle to be kept at 50-70 ℃, stirring for 30-60min, standing and cooling to obtain the microbial nutrient for the biochemical treatment of wastewater.
Understandably, the cobalt chloride hexahydrate is an inorganic substance, is a hydrate of the cobalt chloride and can provide cobalt element necessary for the growth of microorganisms;
the sodium o-nitrophenolate is a dark red needle crystal, is stable at normal temperature, is easy to dissolve in water, can be dissolved in organic solvents such as ethanol, methanol, acetone and the like, and can promote the protoplasm flow of microorganisms;
hemin is an in vitro purification form of natural heme, which is generally separated and purified from animal blood, and can be used as a growth stimulating factor of microorganisms;
the potassium fulvate is purchased from the chemical Limited of Jinnanding, the organic matter is more than or equal to 35 percent, and the mercury is less than or equal to 0.01 percent;
ammonium molybdate tetrahydrate is purchased from Jinlin chemical Limited, chloride is less than or equal to 0.005 percent, and heavy metal is less than or equal to 0.001 percent.
Furthermore, the weight proportions of the wheat root, the distilled water, the ammonium molybdate tetrahydrate, the ferrous sulfate heptahydrate, the potassium fulvate, the cobalt chloride hexahydrate, the sodium o-nitrophenolate, the hemin and the bioactive phosphorus are respectively 33-55 percent of the wheat root, 10-12 percent of the distilled water, 4-6 percent of the ammonium molybdate tetrahydrate, 1-2 percent of the ferrous sulfate heptahydrate, 20-30 percent of the potassium fulvate, 3-5 percent of the cobalt chloride hexahydrate, 1.5-3 percent of the sodium o-nitrophenolate, 0.5-2 percent of the hemin and 5-7 percent of the bioactive phosphorus.
Further, the drying box in the step S1 is a blowing-type drying box, the drying temperature is 60-90 ℃, and the drying time is 1-2 hours.
Further, each ultrasonic extraction time in the step S2 is 1-3h, the inlet temperature in the spray drying device is 100-200 ℃ and the outlet temperature is 30-60 ℃.
Further, in the step S3, the supercritical carbon dioxide extraction pressure is 13-22Mpa, the carbon dioxide flow rate is 10-15kg/h, the extraction temperature is 40-60 ℃, and the extraction time is 15-35 min.
Further, in the step S4, the stirring speed is 60-120r/min, the centrifugal force of the centrifugation is 80000-100000Xg, the centrifugation time is 10-25min, and the centrifugation temperature is 10-30 ℃.
Further, the stirring speed in the step S5 is 100-165r/min, and the cooling time is 3-5 h. Example 1, 33% of wheat root, 12% of distilled water, 6% of ammonium molybdate tetrahydrate, 2% of ferrous sulfate heptahydrate, 30% of potassium fulvate, 5% of cobalt chloride hexahydrate, 3% of sodium o-nitrophenolate, 2% of hemin and 7% of bioactive phosphorus were weighed in proportion by weight, respectively.
A preparation method of a microbial nutrient for wastewater biochemical treatment comprises the following steps:
s1, cleaning the wheat roots, putting the wheat roots into a blowing type drying box for drying at the temperature of 60 ℃ for 1h, crushing the wheat roots after drying, and sieving the wheat roots with a 50-mesh sieve;
s2, adding the screened wheat roots into an ultrasonic extractor, adding 20 times of water, performing ultrasonic extraction for 1 time, wherein the ultrasonic extraction time is 1 hour each time, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure to obtain a wheat root concentrated solution, performing spray drying to obtain a wheat root extract, wherein the inlet temperature of a spray drying device is 100 ℃ and the outlet temperature is 30 ℃;
s3, placing the wheat root extract in a supercritical extraction kettle for supercritical carbon dioxide extraction, wherein the supercritical carbon dioxide extraction pressure is 13Mpa, the carbon dioxide flow rate is 10kg/h, the extraction temperature is 40 ℃, and the extraction time is 15min, so as to obtain an extract;
s4, mixing and stirring potassium fulvate, ammonium molybdate tetrahydrate, ferrous sulfate heptahydrate and distilled water for 20min, controlling the temperature at 30 ℃ and the stirring speed at 60r/min, adding the mixture into a centrifugal machine for centrifugal treatment, wherein the centrifugal force is 80000Xg, the centrifugal time is 10min and the centrifugal temperature is 10 ℃, and taking supernatant;
s5, adding the extract, the supernatant, cobalt chloride hexahydrate, sodium o-nitrophenolate, hemin and bioactive phosphorus into a stirring kettle, controlling the temperature of the stirring kettle to be kept at 50 ℃, stirring at the speed of 100r/min, stirring for 30min, standing and cooling for 3h to obtain the microbial nutrient for biochemical treatment of wastewater.
Example 2, 55% of wheat root, 10% of distilled water, 4% of ammonium molybdate tetrahydrate, 1% of ferrous sulfate heptahydrate, 20% of potassium fulvate, 3% of cobalt chloride hexahydrate, 1.5% of sodium o-nitrophenolate, 0.5% of hemin and 5% of bioactive phosphorus are weighed respectively in weight proportion.
A preparation method of a microbial nutrient for wastewater biochemical treatment comprises the following steps:
s1, cleaning the wheat roots, putting the wheat roots into a blowing type drying box for drying at the temperature of 90 ℃ for 2 hours, crushing the wheat roots after drying, and sieving the wheat roots with a 80-mesh sieve;
s2, adding the screened wheat roots into an ultrasonic extractor, adding 30 times of water, performing ultrasonic extraction for 3 times, wherein the ultrasonic extraction time is 3 hours each time, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure to obtain a wheat root concentrated solution, performing spray drying to obtain a wheat root extract, wherein the inlet temperature and the outlet temperature in a spray drying device are respectively 200 ℃ and 60 ℃;
s3, placing the wheat root extract in a supercritical extraction kettle for supercritical carbon dioxide extraction, wherein the supercritical carbon dioxide extraction pressure is 22Mpa, the carbon dioxide flow rate is 15kg/h, the extraction temperature is 60 ℃, and the extraction time is 35min, so as to obtain an extract;
s4, mixing and stirring potassium fulvate, ammonium molybdate tetrahydrate, ferrous sulfate heptahydrate and distilled water for 30min, controlling the temperature at 50 ℃ and the stirring speed at 120r/min, adding the mixture into a centrifugal machine for centrifugal treatment, wherein the centrifugal force is 100000Xg, the centrifugal time is 25min, and the centrifugal temperature is 30 ℃, and taking supernatant;
s5, adding the extract, the supernatant, cobalt chloride hexahydrate, sodium o-nitrophenolate, hemin and bioactive phosphorus into a stirring kettle, controlling the temperature of the stirring kettle to be kept at 70 ℃, stirring at the speed of 165r/min, stirring for 60min, standing and cooling for 5h to obtain the microbial nutrient for biochemical treatment of wastewater.
Example 3, 44.5% of wheat root, 11% of distilled water, 5% of ammonium molybdate tetrahydrate, 1.5% of ferrous sulfate heptahydrate, 25% of potassium fulvate, 4% of cobalt chloride hexahydrate, 2% of sodium o-nitrophenolate, 1% of hemin and 6% of bioactive phosphorus are weighed respectively in weight proportion.
A preparation method of a microbial nutrient for wastewater biochemical treatment comprises the following steps:
s1, cleaning the wheat roots, putting the wheat roots into a blowing type drying box for drying at the temperature of 80 ℃ for 1.5h, crushing the wheat roots after drying, and sieving the wheat roots with a 70-mesh sieve;
s2, adding the screened wheat roots into an ultrasonic extractor, adding 25 times of water, performing ultrasonic extraction for 2 times, wherein the ultrasonic extraction time is 2 hours each time, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure to obtain a wheat root concentrated solution, performing spray drying to obtain a wheat root extract, wherein the inlet temperature and the outlet temperature in a spray drying device are respectively 150 ℃ and 50 ℃;
s3, placing the wheat root extract in a supercritical extraction kettle for supercritical carbon dioxide extraction, wherein the supercritical carbon dioxide extraction pressure is 18Mpa, the carbon dioxide flow rate is 13kg/h, the extraction temperature is 50 ℃, and the extraction time is 20min, so as to obtain an extract;
s4, mixing and stirring potassium fulvate, ammonium molybdate tetrahydrate, ferrous sulfate heptahydrate and distilled water for 25min, controlling the temperature at 40 ℃ and the stirring speed at 90r/min, adding the mixture into a centrifugal machine for centrifugal treatment, wherein the centrifugal force is 90000Xg, the centrifugal time is 17min, and the centrifugal temperature is 23 ℃, and taking supernatant;
s5, adding the extract, the supernatant, cobalt chloride hexahydrate, sodium o-nitrophenolate, hemin and bioactive phosphorus into a stirring kettle, controlling the temperature of the stirring kettle to be 65 ℃, stirring at the speed of 135r/min, stirring for 45min, standing and cooling for 4h to obtain the microbial nutrient for biochemical treatment of wastewater.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A preparation method of a microbial nutrient for biochemical treatment of wastewater is characterized by comprising the following steps:
s1, cleaning the wheat roots, placing the wheat roots into a drying box for drying, crushing the wheat roots after drying, and sieving the wheat roots with a 50-80-mesh sieve;
s2, adding the screened wheat roots into an ultrasonic extractor, adding 20-30 times of water, performing ultrasonic extraction for 1-3 times, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure to obtain a wheat root concentrated solution, and performing spray drying to obtain a wheat root extract;
s3, placing the wheat root extract in a supercritical extraction kettle for supercritical carbon dioxide extraction to obtain an extract;
s4, mixing and stirring potassium fulvate, ammonium molybdate tetrahydrate, ferrous sulfate heptahydrate and distilled water for 20-30min, controlling the temperature at 30-50 ℃, adding into a centrifuge, centrifuging, and taking supernatant;
s5, adding the extract, the supernatant, cobalt chloride hexahydrate, sodium o-nitrophenolate, hemin and bioactive phosphorus into a stirring kettle, controlling the temperature of the stirring kettle to be kept at 50-70 ℃, stirring for 30-60min, standing and cooling to obtain the microbial nutrient for the biochemical treatment of wastewater.
2. The method for preparing the microbial nutrient for the biochemical treatment of the wastewater according to claim 1, wherein the method comprises the following steps: the weight proportions of the wheat root, the distilled water, the ammonium molybdate tetrahydrate, the ferrous sulfate heptahydrate, the potassium fulvate, the cobalt chloride hexahydrate, the sodium o-nitrophenolate, the hemin and the bioactive phosphorus are respectively 33-55 percent of the wheat root, 10-12 percent of the distilled water, 4-6 percent of the ammonium molybdate tetrahydrate, 1-2 percent of the ferrous sulfate heptahydrate, 20-30 percent of the potassium fulvate, 3-5 percent of the cobalt chloride hexahydrate, 1.5-3 percent of the sodium o-nitrophenolate, 0.5-2 percent of the hemin and 5-7 percent of the bioactive phosphorus.
3. The method for preparing the microbial nutrient for the biochemical treatment of the wastewater according to claim 1, wherein the method comprises the following steps: and the drying box in the step S1 is a blowing type drying box, the drying temperature is 60-90 ℃, and the drying time is 1-2 h.
4. The method for preparing the microbial nutrient for the biochemical treatment of the wastewater according to claim 1, wherein the method comprises the following steps: in the step S2, the ultrasonic extraction time is 1-3h each time, the inlet temperature in the spray drying device is 100-200 ℃ and the outlet temperature is 30-60 ℃.
5. The method for preparing the microbial nutrient for the biochemical treatment of the wastewater according to claim 1, wherein the method comprises the following steps: in the step S3, the supercritical carbon dioxide extraction pressure is 13-22Mpa, the carbon dioxide flow rate is 10-15kg/h, the extraction temperature is 40-60 ℃, and the extraction time is 15-35 min.
6. The method for preparing the microbial nutrient for the biochemical treatment of the wastewater according to claim 1, wherein the method comprises the following steps: in the step S4, the stirring speed is 60-120r/min, the centrifugal force of the centrifugation is 80000-100000Xg, the centrifugation time is 10-25min, and the centrifugation temperature is 10-30 ℃.
7. The method for preparing the microbial nutrient for the biochemical treatment of the wastewater according to claim 1, wherein the method comprises the following steps: in the step S5, the stirring speed is 100-165r/min, and the cooling time is 3-5 h.
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Application publication date: 20211207 |