CN104628149B - A kind of composite flocculation agent of processing slaughtering wastewater - Google Patents

A kind of composite flocculation agent of processing slaughtering wastewater Download PDF

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CN104628149B
CN104628149B CN201410810664.4A CN201410810664A CN104628149B CN 104628149 B CN104628149 B CN 104628149B CN 201410810664 A CN201410810664 A CN 201410810664A CN 104628149 B CN104628149 B CN 104628149B
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blown
composite flocculation
flocculation agent
culture
mixed culture
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CN104628149A (en
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张琰
吴昌春
卢海燕
李才锐
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Negative-ion cooking fume purifier
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Negative-Ion Cooking Fume Purifier
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/22Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
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  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The present invention discloses a kind of composite flocculation agent of processing slaughtering wastewater, the formula of this composite flocculation agent is counted by weight, be made up of 3-5 part mixed culture fermentation bacterium powder, 0.5-1.5 part ferrous sulfate, described mixed culture fermentation bacterium powder is by the mixed culture fermentation bacterium powder of preparing after blown-ball Azotobacter and aspergillus niger mixed culture fermentation. The present invention's mixed culture fermentation under suitable culture medium prescription and condition of culture by blown-ball Azotobacter and aspergillus niger, the mixed culture fermentation bacterium powder obtaining has certain flocculation treatment effect to slaughtering wastewater, and this mixed culture fermentation bacterium powder and ferrous sulfate according to suitable ratio collocation after, better to the removal effect of COD, total phosphorus, turbidity and SS in slaughtering wastewater. After adopting composite flocculation agent of the present invention to process slaughtering wastewater, be conducive to reduce difficulty and the cost of waste water later stage biochemical treatment, also can effectively ensure the up to standard of water quality after wastewater treatment.

Description

A kind of composite flocculation agent of processing slaughtering wastewater
Technical field
The present invention relates to technical field of sewage, relate in particular to the treatment technology of slaughtering wastewater, be specifically related to a kind of placeThe composite flocculation agent of reason slaughtering wastewater.
Background technology
Slaughtering wastewater is the one of livestock products waste water, belongs to high concentrated organic wastewater, mainly come from hutch flushing, drip washing,Butcher and other factory building terrace rinses, scalds hair, analyses, non-staple foodstuff processing, washing oil etc., in its waste water, organic suspended matter content is high, easyCorruption, enters water body and can consume the dissolved oxygen in water, destroys the ecosystem, contaminated environment.
Although China has substantially realized in most of city in the fixed-point set of fowl poultry and having butchered, along with people's living standardImprove constantly, the scale in slaughterhouse is also in continuous expansion, the discharge capacity of slaughtering wastewater is increasing, and slaughtering wastewater needs tightLattice are carried out the regulation such as country and local environmental protection, health and safety, and main water quality index meets relative national standards after treatment, andSlaughtering wastewater has the features such as floorboard with high oil content, high carbon-phosphorus ratio and high carbon-nitrogen ratio, and therefore, intractability is larger.
After selecting suitable flocculant to carry out processing early stage to slaughtering wastewater, be conducive to reduce the biochemical treatment of waste water later stageDifficulty and cost, also can effectively ensure the up to standard of water quality after wastewater treatment. Therefore, researching and developing novel slaughtering wastewater processes with flocculationAgent has great importance and wide market prospects.
Summary of the invention
The object of this invention is to provide a kind of novel processing slaughtering wastewater composite flocculation agent.
Above-mentioned purpose of the present invention is achieved by following scheme:
Process a composite flocculation agent for slaughtering wastewater, the formula of this composite flocculation agent is each group by following parts by weightPart composition:
Mixed culture fermentation bacterium powder 3-5 part;
Ferrous sulfate 0.5-1.5 part.
In the formula of above-mentioned composite flocculation agent, described mixed culture fermentation bacterium powder is by blown-ball Azotobacter and aspergillus niger mixed culture fermentationThe mixed culture fermentation bacterium powder of rear preparation, concrete preparation method comprises the steps:
The activation of step 1. bacterial strain and cultivation
(1) activation of blown-ball Azotobacter and cultivation
Blown-ball Azotobacter bacterial strain is rinsed to inclined-plane with SPSS after activation is cultivated on inclined-plane and obtain blown-ball AzotobacterBacteria suspension, receives this blown-ball Azotobacter bacteria suspension in blown-ball Azotobacter culture medium and trains with the inoculum concentration of volume ratio 10%Support, condition of culture is: 25 DEG C-28 DEG C, shaking table is cultivated 72h, obtains blown-ball Azotobacter nutrient solution;
(2) activation of aspergillus niger and cultivation
Aspergillus niger strain is rinsed to inclined-plane with SPSS after activation is cultivated on inclined-plane and obtain black-koji mould suspension, willThis black-koji mould suspension is received in aspergillus niger culture medium and is carried out seed culture with the inoculum concentration of volume ratio 10%, and condition of culture is:30 DEG C-32 DEG C, shaking table is cultivated 72h, obtains aspergillus niger nutrient solution;
Step 2.
Blown-ball Azotobacter nutrient solution and aspergillus niger nutrient solution prepared by step 1 are inoculated in mixed fermentive culture medium, and 28Under the condition of DEG C-30 DEG C, liquid fermentation and culture, after 4 days, obtains mixing fermentation culture liquid, and this mixing fermentation culture liquid is carried out to bacteriumLiquid separates and obtains bacterium mud, this bacterium mud is sprayed dry, prepares mixed culture fermentation bacterium powder.
In the activation of above-mentioned bacterial strains and cultivation, described blown-ball Azotobacter and aspergillus niger are commercial product.
In the activation and incubation of above-mentioned blown-ball Azotobacter, described inclined-plane is cultivated activation and is adopted those skilled in the art'sRoutine operation, in specific operation process, also can adopt with the following method:
The formula of slant medium is: glucose 10g, NaCl2g, KH2PO40.2g、CaSO4·2H2O0.1g、MgSO40.2g、CaCO35g, agar 20g, distilled water 1000mL, pH7.0; It is 28 DEG C that activation temperature is cultivated on inclined-plane, incubation timeFor 48h.
In the activation and incubation of above-mentioned blown-ball Azotobacter, blown-ball Azotobacter bacterial strain after cultivating activation, inclined-plane is used to nothingBacterium normal saline flushing inclined-plane obtains blown-ball Azotobacter bacteria suspension, and the cell concentration of described blown-ball Azotobacter bacteria suspension is 5 ×108cfu/mL。
In the activation and incubation of above-mentioned blown-ball Azotobacter, the formula of described blown-ball Azotobacter culture medium is:KH2PO40.2g、K2HPO40.8g、MgSO4.7H2O0.2g、CaSO40.1g、Na2MoO4.2H2O trace, FeCl3Trace, yeast extract0.5g, sweet mellow wine 20g, distilled water 1000mL, agar 15g, pH7.2.
In the activation and incubation of above-mentioned aspergillus niger, activation employing those skilled in the art's routine is cultivated on described inclined-planeOperation, in specific operation process, also can adopt with the following method:
The formula of slant medium is: NaNO32.0g、K2HPO41.0g、KCl0.5g、MgSO40.5g、FeSO40.01g、Sucrose 30g, agar 20g, distilled water 1000mL, pH nature; It is 30 DEG C that activation temperature is cultivated on inclined-plane, and incubation time is 48h.
In the preparation method of above-mentioned black-koji mould powder, Aspergillus niger strain after cultivating activation, inclined-plane is used to SPSSRinse inclined-plane and obtain black-koji mould suspension, the cell concentration of described black-koji mould suspension is 6 × 108cfu/mL。
In the activation and incubation of above-mentioned aspergillus niger, the formula of described aspergillus niger culture medium is: cane molasses 3g, urea6g, corn protein powder 2g, K2HPO40.5g、MgSO4.7H2O0.06g, distilled water 1000mL, pH6.0.
In above-mentioned preparation method's step 2, blown-ball Azotobacter nutrient solution is to be inoculated into mixing with the inoculum concentration of volume ratio 5%In fermentation medium; Aspergillus niger nutrient solution is to be inoculated in mixed fermentive culture medium with the inoculum concentration of volume ratio 5%; Brown ball fixed nitrogenBacteria culture fluid and aspergillus niger nutrient solution are to be inoculated in same mixed fermentive culture medium, the formula of described mixed fermentive culture mediumFor: wheat bran 0.5g, corn flour 15g, glucose 5g, FeSO4.7H2O4g、K2HPO42.5g、KH2PO41g, distilled water 1000mL,pH7.0。
In above-mentioned preparation method, described spray-dired intake air temperature is 160 DEG C, and air outlet temperature is 60 DEG C, described inIn mixed culture fermentation bacterium powder, water content is not higher than 10%.
Above-mentioned a kind of composite flocculation agent of processing slaughtering wastewater, the preferred version of the formula of this composite flocculation agent is by as followsThe each component composition of parts by weight:
4 parts, mixed culture fermentation bacterium powder;
1 part, ferrous sulfate.
A kind of composite flocculation agent of processing slaughtering wastewater of the present invention, its preparation method comprises the steps:
Mixed culture fermentation bacterium powder will be prepared according to preceding method after blown-ball Azotobacter and aspergillus niger mixed culture fermentation;
After being ground, ferrous sulfate crosses 100 mesh sieves;
By mixed culture fermentation bacterium powder and ferrous sulfate according to formula consumption mix prepare a kind of process butcher uselessThe composite flocculation agent of water.
Through experiment test, a kind of composite flocculation agent of processing slaughtering wastewater of the present invention can be for the treatment of slaughtering wastewater,COD in slaughtering wastewater, colourity, SS are had to good clearance, ammonia nitrogen is also had to good clearance; The present inventionThe amount of application of composite flocculation agent in slaughtering wastewater is 0.25g/L-0.5g/L.
Compared with prior art, the present invention has following beneficial effect:
1. for the high phenomenon of slaughtering wastewater content of organics, the present invention first by blown-ball Azotobacter and aspergillus niger through mixingAfter fermentation, for the flocculation treatment of slaughtering wastewater, and obtain beyond thought good flocculation treatment effect;
2. the selection of its formula of mixed fermentive culture medium of the present invention has ensured that blown-ball Azotobacter and aspergillus niger not only canMixed culture fermentation, and fermentation after bacterium mud can realize object of the present invention;
3. the present invention finds by research, by blown-ball Azotobacter and aspergillus niger at suitable culture medium prescription and condition of cultureLower mixed culture fermentation, the mixed culture fermentation bacterium powder obtaining has certain flocculation treatment effect to slaughtering wastewater, and this mixed culture fermentation bacteriumAfter powder and ferrous sulfate are arranged in pairs or groups according to suitable ratio, to the removal effect of COD, colourity, SS and ammonia nitrogen in slaughtering wastewaterFruit is better;
4., after adopting composite flocculation agent of the present invention to process slaughtering wastewater, be conducive to reduce biochemical place of waste water later stageDifficulty and the cost of reason, also can effectively ensure the up to standard of water quality after wastewater treatment.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is done further and described, but specific embodiment is not appointed the present inventionWhat limits.
Activation and the cultivation of embodiment 1 blown-ball Azotobacter
The blown-ball Azotobacter of the present embodiment adopts commercial product---blown-ball Azotobacter ACCC10096.
Blown-ball Azotobacter bacterial strain is first carried out to inclined-plane and cultivate activation, the formula of slant medium is: glucose 10g, NaCl2g、KH2PO40.2g、CaSO4·2H2O0.1g、MgSO40.2g、CaCO35g, agar 20g, distilled water 1000mL, pH7.0; TiltedlyIt is 28 DEG C that face is cultivated activation temperature, and incubation time is 48h; Inclined-plane rinses inclined-plane with SPSS after cultivating and activating and obtains brownBall nitrogen-fixing bacteria bacteria suspension, the cell concentration of described blown-ball Azotobacter bacteria suspension is 5 × 108cfu/mL;
Above-mentioned blown-ball Azotobacter bacteria suspension is received in blown-ball Azotobacter culture medium and carried out with the inoculum concentration of volume ratio 10%Cultivate, condition of culture is: 25 DEG C-28 DEG C, shaking table is cultivated 72h, obtains blown-ball Azotobacter nutrient solution; Described blown-ball Azotobacter is cultivatedThe formula of base is KH2PO40.2g、K2HPO40.8g、MgSO4.7H2O0.2g、CaSO40.1g、Na2MoO4.2H2O trace, FeCl3Trace, yeast extract 0.5g, sweet mellow wine 20g, distilled water 1000mL, agar 15g, pH7.2.
Activation and the cultivation of embodiment 2 aspergillus nigers
The present embodiment aspergillus niger adopts commercial product---aspergillus niger ATCC10864.
Aspergillus niger strain is first carried out to inclined-plane and cultivate activation, the formula of slant medium is: NaNO32.0g、K2HPO41.0g、KCl0.5g、MgSO40.5g、FeSO40.01g, sucrose 30g, agar 20g, distilled water 1000mL, pH nature;It is 30 DEG C that activation temperature is cultivated on inclined-plane, and incubation time is 48h; Inclined-plane rinses inclined-plane with SPSS after cultivating and activating and obtainsBlack-koji mould suspension, the cell concentration of described black-koji mould suspension is 6 × 108cfu/mL;
Above-mentioned black-koji mould suspension is received and in aspergillus niger culture medium, is carried out seed culture with the inoculum concentration of volume ratio 10%,Condition of culture is: 30 DEG C-32 DEG C, shaking table is cultivated 72h, obtains aspergillus niger nutrient solution; The formula of described aspergillus niger culture medium is: sweetCane molasses 3g, urea 6g, corn protein powder 2g, K2HPO40.5g、MgSO4.7H2O0.06g, distilled water 1000mL, pH6.0.
Embodiment 3 mixed culture fermentations
Prepare mixed fermentive culture medium, its formula is wheat bran 0.5g, corn flour 15g, glucose 5g, FeSO4.7H2O4g、K2HPO42.5g、KH2PO41g, distilled water 1000mL, pH7.0.
Blown-ball Azotobacter nutrient solution prepared by embodiment 1 is inoculated into above-mentioned mixed culture fermentation with the inoculum concentration of volume ratio 5%In culture medium, and aspergillus niger nutrient solution prepared by embodiment 2 is also inoculated into this mixed culture fermentation with the inoculum concentration of volume ratio 5%In culture medium, under the condition of 28 DEG C-30 DEG C, liquid fermentation and culture, after 4 days, obtains mixing fermentation culture liquid, by this mixed culture fermentationNutrient solution carries out the separation of bacterium liquid and obtains bacterium mud, this bacterium mud is sprayed dry, and spray-dired intake air temperature is 160 DEG C,Air outlet temperature is 60 DEG C, prepares mixed culture fermentation bacterium powder, and in this mixed culture fermentation bacterium powder, water content is not higher than 10%.
Embodiment 4 blown-ball Azotobacter bacterium powder
The blown-ball Azotobacter bacterium powder of the present embodiment is for contrast experiment, and its fermentation medium adopts mixing of embodiment 3Close fermentative medium formula, blown-ball Azotobacter nutrient solution prepared by embodiment 1 is inoculated into mixing with the inoculum concentration of volume ratio 5%In fermentation medium, under the condition of 28 DEG C-30 DEG C, liquid fermentation and culture, after 4 days, obtains blown-ball Azotobacter zymotic fluid, and this is brownBall nitrogen-fixing bacteria zymotic fluid carries out the separation of bacterium liquid and obtains bacterium mud, this bacterium mud sprayed dry, and spray-dired intake air temperatureBe 160 DEG C, air outlet temperature is 60 DEG C, prepares blown-ball Azotobacter bacterium powder, and in this blown-ball Azotobacter bacterium powder, water content is notHigher than 10%.
Embodiment 5 black-koji mould powder
The black-koji mould powder of the present embodiment is for contrast experiment, and its fermentation medium adopts the mixing of embodiment 3 to send outFerment culture medium prescription, aspergillus niger nutrient solution prepared by embodiment 1 is inoculated into mixing fermentation culture with the inoculum concentration of volume ratio 5%In base, under the condition of 28 DEG C-30 DEG C, liquid fermentation and culture, after 4 days, obtains fermentation of Aspergillus niger liquid, and this fermentation of Aspergillus niger liquid is enteredRow bacterium liquid separates and obtains bacterium mud, this bacterium mud is sprayed dry, and spray-dired intake air temperature is 160 DEG C, air outlet temperatureDegree is 60 DEG C, prepares black-koji mould powder, and in this black-koji mould powder, water content is not higher than 10%.
Embodiment 6 performance test experiments
1, laboratory sample
The laboratory sample of the present embodiment is divided into experimental group 1, experimental group 2, contrast groups 1, contrast groups 2 and contrast groups 3, concrete asShown in lower:
(1) experimental group 1
After being ground, ferrous sulfate crosses 100 mesh sieves, according to following formula consumption, and mixed culture fermentation bacterium prepared by embodiment 3After mixing, powder and ferrous sulfate prepare the laboratory sample of experimental group 1.
The formula of experimental group 1 is made up of the each component of following parts by weight:
3 parts, mixed culture fermentation bacterium powder;
1.5 parts, ferrous sulfate.
(2) experimental group 2
After being ground, ferrous sulfate crosses 100 mesh sieves, according to following formula consumption, and mixed culture fermentation bacterium prepared by embodiment 3After mixing, powder and ferrous sulfate prepare the laboratory sample of experimental group 2.
The formula of experimental group 2 is made up of the each component of following parts by weight:
4 parts, mixed culture fermentation bacterium powder;
1 part, ferrous sulfate.
(3) contrast groups 1
Black-koji mould powder prepared by blown-ball Azotobacter bacterium powder prepared by embodiment 4 and embodiment 5 is according to weight ratio 1:1'sRatio mixes and obtains Mixed Microbes powder;
After being ground, ferrous sulfate crosses 100 mesh sieves, according to following formula consumption, by mixed to above-mentioned Mixed Microbes powder and ferrous sulfateAfter closing evenly, prepare the laboratory sample of contrast groups 1.
The formula of contrast groups 1 is made up of the each component of following parts by weight:
4 parts, Mixed Microbes powder;
1 part, ferrous sulfate.
(4) contrast groups 2
Mixed culture fermentation bacterium powder prepared by embodiment 3 is the laboratory sample of contrast groups 2.
(5) contrast groups 3
Rear 100 mesh sieves excessively of ferrous sulfate grinding are the laboratory sample of contrast groups 3.
2, experimentation and result
Taking local certain 1,100,000 first-born pig/year slaughterhouse slaughtering wastewater as experimental subjects, the slaughtering wastewater warp in this slaughterhouseDetect COD (COD in its waste watercr) concentration be that the concentration of 1800mg/L, SS is 1000mg/L, ammonia nitrogen (NH3-N)Concentration be that 30mg/L, colourity are 300 times.
This slaughtering wastewater is divided into 5 parts, and every part of 1L adds respectively above-mentioned experimental group 1, experimental group in every part of slaughtering wastewater2, the laboratory sample of contrast groups 1, contrast groups 2 and contrast groups 3, addition is 0.25g/L, after stirring 20min, leaves standstill, and leaves standstillAfter 30min, detect, result is as shown in table 1.
Table 1 flocculating effect statistics
As can be seen from Table 1, compared with single fermentation, the present invention by after blown-ball Azotobacter and aspergillus niger mixed culture fermentation to slaughterThe removal effect of killing COD, SS, colourity and ammonia nitrogen in waste water increases greatly, and mixed culture fermentation bacterium powder collocation sulfuric acid AsiaAfter iron, its removal effect to COD, SS, colourity and ammonia nitrogen in slaughtering wastewater is more excellent.

Claims (10)

1. process a composite flocculation agent for slaughtering wastewater, the formula that it is characterized in that this composite flocculation agent is by following weight portionThe each component composition of number:
Mixed culture fermentation bacterium powder 3-5 part;
Ferrous sulfate 0.5-1.5 part;
Above-mentioned mixed culture fermentation bacterium powder is by the mixed culture fermentation bacterium powder of preparing after blown-ball Azotobacter and aspergillus niger mixed culture fermentation;
The mixed fermentive culture medium formula that above-mentioned mixed culture fermentation adopts is: wheat bran 0.5g, corn flour 15g, glucose 5g,FeSO4·7H2O4g、K2HPO42.5g、KH2PO41g, distilled water 1000mL, pH7.0; Mixed culture fermentation temperature is 28 DEG C-30DEG C, the mixed culture fermentation time is 4 days.
2. a kind of composite flocculation agent of processing slaughtering wastewater according to claim 1, is characterized in that described composite flocculation agentFormula formed by the each component of following parts by weight:
4 parts, mixed culture fermentation bacterium powder;
1 part, ferrous sulfate.
3. according to a kind of composite flocculation agent of processing slaughtering wastewater described in claim 1 or 2, it is characterized in that described mixed culture fermentationThe preparation method of bacterium powder comprises the steps:
The activation of step 1. bacterial strain and cultivation
(1) activation of blown-ball Azotobacter and cultivation
Blown-ball Azotobacter bacterial strain after cultivating activation, inclined-plane is obtained to blown-ball Azotobacter bacterium with SPSS flushing inclined-plane outstandingLiquid, receives this blown-ball Azotobacter bacteria suspension in blown-ball Azotobacter culture medium and cultivates with the inoculum concentration of volume ratio 10%, cultivatesCondition is: 25 DEG C-28 DEG C, shaking table is cultivated 72h, obtains blown-ball Azotobacter nutrient solution;
(2) activation of aspergillus niger and cultivation
Aspergillus niger strain is rinsed to inclined-plane with SPSS after activation is cultivated on inclined-plane and obtain black-koji mould suspension, this is blackAspergillus suspension is received in aspergillus niger culture medium and is carried out seed culture with the inoculum concentration of volume ratio 10%, and condition of culture is: 30 DEG C-32 DEG C, shaking table is cultivated 72h, obtains aspergillus niger nutrient solution;
Step 2.
Blown-ball Azotobacter nutrient solution and aspergillus niger nutrient solution prepared by step 1 are inoculated in mixed fermentive culture medium, 28 DEG C-30DEG C condition under, liquid fermentation and culture, after 4 days, obtains mixing fermentation culture liquid, this mixing fermentation culture liquid is carried out to bacterium liquid and divideFrom obtaining bacterium mud, this bacterium mud is sprayed dry, prepare mixed culture fermentation bacterium powder.
4. a kind of composite flocculation agent of processing slaughtering wastewater according to claim 3, is characterized in that described blown-ball AzotobacterActivation and incubation in, the formula of slant medium is: glucose 10g, NaCl2g, KH2PO40.2g、CaSO4·2H2O0.1g、MgSO40.2g、CaCO35g, agar 20g, distilled water 1000mL, pH7.0; Activation temperature is cultivated on inclined-plane28 DEG C, incubation time is 48h; The cell concentration of blown-ball Azotobacter bacteria suspension is 5 × 108cfu/mL。
5. a kind of composite flocculation agent of processing slaughtering wastewater according to claim 3, is characterized in that described blown-ball AzotobacterThe formula of culture medium is: KH2PO40.2g、K2HPO40.8g、MgSO4·7H2O0.2g、CaSO40.1g、Na2MoO4·2H2O trace, FeCl3Trace, yeast extract 0.5g, sweet mellow wine 20g, distilled water 1000mL, agar 15g, pH7.2.
6. a kind of composite flocculation agent of processing slaughtering wastewater according to claim 3, is characterized in that the work of described aspergillus nigerIn change and incubation, the formula of slant medium is: NaNO32.0g、K2HPO41.0g、KCl0.5g、MgSO40.5g、FeSO40.01g, sucrose 30g, agar 20g, distilled water 1000mL, pH nature; It is 30 that activation temperature is cultivated on inclined-planeDEG C, incubation time is 48h; The cell concentration of black-koji mould suspension is 6 × 108cfu/mL。
7. a kind of composite flocculation agent of processing slaughtering wastewater according to claim 3, is characterized in that described aspergillus niger cultivatesThe formula of base is: cane molasses 3g, urea 6g, corn protein powder 2g, K2HPO40.5g、MgSO4·7H2O0.06g, steamingHeat up in a steamer water 1000mL, pH6.0.
8. a kind of composite flocculation agent of processing slaughtering wastewater according to claim 3, is characterized in that in described step 2, brownBall nitrogen-fixing bacteria nutrient solution is to be inoculated in mixed fermentive culture medium with the inoculum concentration of volume ratio 5%; Aspergillus niger nutrient solution is with volumeInoculum concentration than 5% is inoculated in mixed fermentive culture medium.
9. a kind of composite flocculation agent of processing slaughtering wastewater according to claim 3, is characterized in that described spray-diredIntake air temperature is 160 DEG C, and air outlet temperature is 60 DEG C.
10. a kind of composite flocculation agent of processing slaughtering wastewater according to claim 3, is characterized in that described composite flocculation agentAmount of application in slaughtering wastewater is 0.25g/L-0.5g/L.
CN201410810664.4A 2014-12-23 2014-12-23 A kind of composite flocculation agent of processing slaughtering wastewater Active CN104628149B (en)

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