CN105110486B - A kind of method that tetracycline in water removal is removed with microbial flocculant - Google Patents
A kind of method that tetracycline in water removal is removed with microbial flocculant Download PDFInfo
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Abstract
A kind of method that tetracycline in water removal is removed with microbial flocculant, it is related to a kind of method that tetracycline in water removal is removed with microbial flocculant.The invention solves the problems that in traditional treatment method the problem of secondary pollution.The method of the present invention is one, microbial flocculant and flocculation aid is added into tetracycline water, obtains mixed solution；Wherein, the volume ratio of microbial flocculant and tetracycline water is 3：100；The volume ratio of flocculation aid and tetracycline water is 9：10000；2nd, shaking table is put into after regulation mixed solution pH value, shaken after training, taken out to stand and filtered after 5min, survey liquid phase.The method of the present invention removes the clearance of tetracycline up to 70~80%.
Description
Technical field
The present invention relates to a kind of method that tetracycline in water removal is removed with microbial flocculant.
Background technology
Late 1990s, antibiotic is as a kind of new pollutant in environment, its pollution and harm to environment
Start the extensive concern by foreign scholar.Human body or animal-use drug are the topmost sources of antibiotic, and medicine is through human body or dynamic
After thing intake, in fact only small part is metabolized, and major part is in the form of parent compound or metabolism body through animal wastes
Excreted with urine, into breeding wastewater or the water body environment medium on periphery.Antibioticses drug contamination pair in water environment
The influence of health is mainly manifested at following 2 points：Polluted drinking water simultaneously may have three-induced effect, intestinal bacilli illness etc. to dive
In harm；Contaminated soil and food, the final indirect hazard mankind.Wherein, the antibiotic such as Tetracyclines, fluoroquinolones is extensive
For livestock and poultry cultivation, in prevention and treatment disease, promote to have played aobvious in terms of growth of animal and raising food utilization efficiency
The effect of work, the method tool as tetracycline in the indispensable factor of modern farming enterprise's aquaculture, therefore further investigation processing water
It is significant.Because current sewage treatment process thoroughly can not effectively remove antibiotic contamination, part is undegradable
Active constituents of medicine is just ultimately discharged into natural water body with treated sewage, or is adsorbed in activated sludge, passes through the agricultures such as fertilising
Industry production activity eventually enters into natural environment, therefore it is extremely urgent to find antibiotic in a kind of new method processing water.
The content of the invention
The problem of purpose of the present invention is in order to solve secondary pollution in traditional treatment method, and there is provided a kind of green, height
Effect, the method for non-secondary pollution-go to remove water the plain method of moderate resistance life using microbial flocculant, and applied to the processing of tetracycline
In.
A kind of method that tetracycline in water removal is removed with microbial flocculant of the present invention, it is to follow the steps below
's：
First, microbial flocculant and flocculation aid are added into tetracycline water, mixed solution is obtained；Wherein, microbial flocculant
Volume ratio with tetracycline water is 3：100；The volume ratio of flocculation aid and tetracycline water is 9：10000；
2nd, shaking table is put into after regulation mixed solution pH value, shakes after 0.1~90min of training, take out and stand after 5min, that is, complete；
Wherein, the preparation process of microbial flocculant is as follows：It is 1g according to mass volume ratio：480~520mL ratio will
Microbial flocculant MFX dry powder is mixed with distilled water, is placed on magnetic stirring apparatus, and the microorganism that concentration is 2.0g/L is obtained after stirring
Flocculant；
The concentration of described tetracycline water is 75~85 μ g/L.
The present invention includes following beneficial effect：
The method of the present invention removes the clearance of tetracycline up to 70~80%.
By further response surface optimization, tetracycline clearance scope can bring up to 75%~80%.
By scanning electron microscopic observation it can be found that the specific surface area of microbial flocculant in itself is larger, micropore is more, is four
Ring element provides more adsorption site, and the flco after absorption then substantially becomes consolidation, and adsorption site is occupied, and shows adsorption effect
Preferably.
Brief description of the drawings
Fig. 1 is microbial flocculant in embodiment 1 to 80 μ g/L tetracycline removal effect figures.
Embodiment
Embodiment one：A kind of method that tetracycline in water removal is removed with microbial flocculant of present embodiment, it
Follow the steps below：
First, microbial flocculant and flocculation aid are added into tetracycline water, mixed solution is obtained；Wherein, microbial flocculant
Volume ratio with tetracycline water is 3：100；The volume ratio of flocculation aid and tetracycline water is 9：10000；
2nd, shaking table is put into after regulation mixed solution pH value, shakes after 0.1~90min of training, take out and stand after 5min, that is, complete；
Wherein, the preparation process of microbial flocculant is as follows：It is 1g according to mass volume ratio：480~520mL ratio will
Microbial flocculant MFX dry powder is mixed with distilled water, is placed on magnetic stirring apparatus, and the microorganism that concentration is 2.0g/L is obtained after stirring
Flocculant；
The concentration of described tetracycline water is 75~85 μ g/L.
Embodiment two：Present embodiment from unlike embodiment one：Helping described in step one is solidifying
Agent is the CaCl that mass fraction is 10%2Solution.It is other identical with embodiment one.
Embodiment three：Present embodiment from unlike embodiment one：In tetracycline water process for preparation
Solvent is used as using ultra-pure water.It is other identical with embodiment one.
Embodiment four：Present embodiment from unlike embodiment one：Described microbial flocculant
For microbial flocculant MFX, wherein microbial flocculant MFX is by preserving number:CGMCC No.6243, depositary institution is：China
Microbiological Culture Collection administration committee common micro-organisms center, preservation address is：City of BeiJing, China Chaoyang District North Star West Road 1
Institute, preservation date is：What on 06 20th, 2012 Klebsiella (Klebsiellasp.) MFX was produced.Other and specific reality
Apply mode one identical.
Embodiment five：Present embodiment from unlike embodiment one：Microbial flocculant MFX dry powder
Preparation method is：
First, Klebsiella (Klebsiellasp.) MFX bacterial strains are activated first and are fermented, and obtain zymotic fluid；
2nd, it is the anhydrous second after 95% 4 DEG C of precoolings that the weight/mass percentage composition of 2 times of fermentating liquid volumes is added into zymotic fluid
Alcohol, after stirring, the white flco in solution is collected by filtration；
3rd, the operation of repeat step two, merges white flco；
4th, the white flco that step 3 is obtained is placed in bag filter, then bag filter is immersed in distilled water carry out it is saturating
Analysis, 24h is placed in room temperature, is put into sets temperature to freeze 24h in -80 DEG C of ultra low temperature freezer afterwards, place into freeze dryer
Dry powder is frozen into, microbial flocculant MFX dry powder is produced；
Described activation Klebsiella (Klebsiellasp.) MFX bacterial strains use culture medium prescription for：Peptone
5.0g, glucose 10.0g, malt extract 3.0g, yeast extract 3.0g, agar 20.0g and water 1000mL；
The culture medium prescription that described fermentation Klebsiella (Klebsiellasp.) MFX bacterial strains are used for：Glucose
10.0g、K2HPO45.0g、NaCl 0.1g、MgSO40.2g, urea 0.5g, yeast extract 0.5g and water 1000mL, pH is 7.2~
7.5, sterilize 30min at 112 DEG C.
It is other identical with embodiment one.
Embodiment six：Present embodiment from unlike embodiment one：The concentration of tetracycline water is 80 μ
g/L.It is other identical with embodiment one.
Embodiment seven：Present embodiment from unlike embodiment one：The preparation of microbial flocculant
Process is as follows：It is 1g according to mass volume ratio：500mL ratio mixes microbial flocculant MFX dry powder with distilled water, is placed in
The microbial flocculation agent solution that concentration is 2.0g/L is obtained on magnetic stirring apparatus, after stirring.It is other identical with embodiment one.
Embodiment eight：Present embodiment from unlike embodiment one：Described regulation mixed solution
PH value refers to adjust mixed solution pH to 8.0.It is other identical with embodiment one.
Embodiment nine：Present embodiment from unlike embodiment one：Described filtering is to use
What 0.45 μm of fibrous filter membrane was filtered.It is other identical with embodiment one.
Embodiment ten：Present embodiment from unlike embodiment one：Shaking the training time in step 2 is
50min.It is other identical with embodiment one.
Present invention is not limited only to the content of the respective embodiments described above, the group of one of them or several embodiments
Contract sample can also realize the purpose of invention.
Beneficial effects of the present invention are verified by following examples：
Embodiment 1
The method that tetracycline in water removal is removed using microbial flocculant, detailed process is：
Activated strains and fermented first, microbial flocculant is extracted from zymotic fluid and freeze-dried powder is prepared；Weigh 1g
Microbial flocculant MFX dry powder is dissolved in 500mL distilled water, is placed on magnetic stirring apparatus and is configured to 2.0g/L solution.Then,
Addition 2.0g/L microbial flocculation agent solution 3.0mL, 90.0 μ L mass fractions are in 80 μ g/L tetracycline water of preparation
10% flocculation aid CaCl2Solution, regulation solution ph is 8.0, carries out shaking table and shakes 0.1~90min of training, takes out and stand after 5min
Filtered with 0.45 μm of fibrous filter membrane, tetracycline concentration is determined using high performance liquid chromatography；
Described microbial flocculant is microbial flocculant MFX, and wherein microbial flocculant MFX is by preserving number:
CGMCC No.6243, depositary institution is：China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address
For：City of BeiJing, China Chaoyang District North Star West Road 1 institute, preservation date is：The Klebsiella of on 06 20th, 2012
(Klebsiellasp.) MFX is produced.
Wherein activation is with culture medium prescription：Peptone 5.0g, glucose 10.0g, malt extract 3.0g, yeast extract 3.0g,
Agar 20.0g, water 1000mL；Fermentative medium formula is：Glucose 10.0g, K2HPO45.0g、NaCl 0.1g、
MgSO40.2g, urea 0.5g, yeast extract 0.5g, water 1000mL, regulation pH value are 7.5.
Through the treatment conditions in embodiment 1：Flocculant dosage is 3.0mL, and flocculation aid dosage is 90.0 μ L, and pH value is
8.0, flocculation time is under conditions of 50min, microbial flocculant is good to the treatment effect of tetracycline in water, and clearance is reachable
71.68%.
The microbial flocculant of the present embodiment is as shown in Figure 1 to 80 μ g/L tetracycline removal effect figures.
Choi etc. removes 100mg/L TAS using aluminium polychloride as the water process coagulation process of water treatment agent, removes
Rate uses active carbon absorption technology to remove tetracycline antibiotics (TAS) and found in the range of 19%~66%, and its is right
10mg/L TAS clearances are higher than 68%.Although traditional treatment method effect is also preferable, secondary pollution is easily produced.And
Its tetracycline clearance is less than the removal effect of the present embodiment.
Claims (8)
1. a kind of method that tetracycline in water removal is removed with microbial flocculant, it is characterised in that it is to follow the steps below
's：
First, microbial flocculant and flocculation aid are added into tetracycline water, mixed solution is obtained；Wherein, microbial flocculant and four
The volume ratio of ring element water is 3：100；The volume ratio of flocculation aid and tetracycline water is 9：10000；
2nd, shaking table is put into after regulation mixed solution pH value, shakes after 0.1~90min of training, take out and stand 5min, that is, complete；
Wherein, the preparation process of microbial flocculant is as follows：It is 1g according to mass volume ratio：480~520mL ratio is by micro- life
Thing flocculant MFX dry powder is mixed with distilled water, is placed on magnetic stirring apparatus, and the microbial flocculation that concentration is 2.0g/L is obtained after stirring
Agent；
The concentration of described tetracycline water is 75~85 μ g/L；Described microbial flocculant is microbial flocculant MFX, wherein
Microbial flocculant MFX is by preserving number:CGMCC No.6243, depositary institution is：Chinese microorganism strain preservation conservator
Understand common micro-organisms center, preservation address is：City of BeiJing, China Chaoyang District North Star West Road 1 institute, preservation date is：2012 06
What Klebsiella (Klebsiella sp.) MFX on the moon 20 was produced.
2. a kind of method that tetracycline in water removal is removed with microbial flocculant according to claim 1, it is characterised in that step
Flocculation aid described in rapid one is the CaCl that mass fraction is 10%2Solution.
3. a kind of method that tetracycline in water removal is removed with microbial flocculant according to claim 1, it is characterised in that four
Ultra-pure water is used as solvent in ring element water process for preparation.
4. a kind of method that tetracycline in water removal is removed with microbial flocculant according to claim 1, it is characterised in that four
The concentration of ring element water is 80 μ g/L.
5. a kind of method that tetracycline in water removal is removed with microbial flocculant according to claim 1, it is characterised in that micro-
The preparation process of biological flocculant is as follows：It is 1g according to mass volume ratio：500mL ratio is by microbial flocculant MFX dry powder
Mix, be placed on magnetic stirring apparatus with distilled water, the microbial flocculation agent solution that concentration is 2.0g/L is obtained after stirring.
6. a kind of method that tetracycline in water removal is removed with microbial flocculant according to claim 1, it is characterised in that institute
The regulation mixed solution pH value stated refers to adjust mixed solution pH to 8.0.
7. a kind of method that tetracycline in water removal is removed with microbial flocculant according to claim 1, it is characterised in that step
The training time is shaken in rapid two for 50min.
8. a kind of method that tetracycline in water removal is removed with microbial flocculant according to claim 1, it is characterised in that micro-
Biological flocculant MFX process for preparation of dry powder is：
First, Klebsiella (Klebsiella sp.) MFX bacterial strains are activated first and are fermented, and obtain zymotic fluid；
2nd, it is the absolute ethyl alcohol after 95% 4 DEG C of precoolings that the weight/mass percentage composition of 2 times of fermentating liquid volumes is added into zymotic fluid,
After stirring, the white flco in solution is collected by filtration；
3rd, the operation of repeat step two, merges white flco；
4th, the white flco that step 3 is obtained is placed in bag filter, then bag filter is immersed in distilled water dialysed, in
Room temperature places 24h, is put into sets temperature to freeze 24h in -80 DEG C of ultra low temperature freezer afterwards, place into freeze dryer be frozen into it is dry
Powder, produces microbial flocculant MFX dry powder；
Described activation Klebsiella (Klebsiella sp.) MFX bacterial strains use culture medium prescription for：Peptone 5.0g, Portugal
Grape sugar 10.0g, malt extract 3.0g, yeast extract 3.0g, agar 20.0g and water 1000mL；
The culture medium prescription that described fermentation Klebsiella (Klebsiella sp.) MFX bacterial strains are used for：Glucose
10.0g、K2HPO4 5.0g、NaCl 0.1g、MgSO40.2g, urea 0.5g, yeast extract 0.5g and water 1000mL, pH is 7.2~
7.5, sterilize 30min at 112 DEG C.
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| CN107459114A (en) * | 2017-09-25 | 2017-12-12 | 吉林大学 | A kind of method of pecan shell electric flocculation coupling processing Tetracyclines simulated wastewater |
| CN107858305B (en) * | 2017-11-28 | 2020-08-14 | 盐城师范学院 | Bacterium for efficiently degrading tetracycline and application thereof |
| CN110803826A (en) * | 2019-10-15 | 2020-02-18 | 广州市凯卫莎环保科技有限公司 | System for treating sewage by adopting microorganisms and treatment method thereof |
| CN111693687B (en) * | 2020-05-28 | 2021-09-17 | 长沙都正生物科技股份有限公司 | Preparation method of biological negative sample and biological standard substance and TMAO detection method |
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| CN104403965B (en) * | 2014-11-03 | 2017-11-17 | 中国地质大学（武汉） | A kind of roost rose of Sharon pseudomonad of water body tetracycline pollutant of degrading and its application |
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