(2) background technology
Along with fish culture scale expands increasingly, disease problem is also day by day serious, causes serious harm to China's aquaculture, and the economic loss causing is every year huge.Because fish live in water body environment complicated and changeable, and mostly fish disease is to use antibiotic and chemotherapy for many years, consequently not only kill pathogen, also killed a large amount of beneficial bacteriums simultaneously, make in water body environment normal flora out of proportion, cause environmental deterioration.Along with endurance strain is on the increase, abuse of antibiotics, the pollution of the caused medicament residue of chemicals to water body environment and day by day serious to the harm of cultured fishes health, causes serious threat to the health of fish.
As the saying goes " breed fish and first support water ", in recent years, the application in aquaculture of substrate modifier and water conditioner is very general, and especially in the cultivation of Bohai Sea Gulf fresh water shrimp, application is wider.At present, on market, conventional substrate modifier has: the probios such as bacillus, enterococcus faecalis; Enzyme preparation, adsorbent, synergist; Shitosan, fulvic acid, sodium humate, bentonite, quicklime and chlorinated product etc.These products to a certain extent all can regulating water quality, improve substrate, but respectively has deficiency, and quicklime is simply effective, but complex operation, and effect is unstable; Chlorinated product effect is better, but excitant is large, contaminated environment; Enzyme preparation and probiotics need regularly to add, and cost is higher, and quality is unstable.
Therefore, in aquaculture, in the urgent need to developing a kind of green, safe and effective substrate modifier, especially particle is granulated, not thorough to solve pond bottom sterilization, the problem that bottom material of pond for breeding worsens.
(3) summary of the invention
The object of the invention is to solve bottom material of pond for breeding sterilization not thorough, the problem that bottom material of pond for breeding worsens, by a kind of bacteria resistance function of Chinese medicine composition, provide a kind of for the green of sediment sterilization, safely, the Chinese herbal medicine granular preparation that has no side effect and preparation method thereof.
The present invention is achieved through the following technical solutions:
A kind of sterilization comprises the steps: by the preparation method of herbal composite
1. filter out the traditional Chinese medicine that can suppress Aeromonas hydrophila and vibrio parahaemolytious, rheum officinale, golden cypress, the coptis, cloves, peppermint, lemongrass, thyme.
2. by the multiple medicinal material filtering out, by orthogonal experiment, carry out the bacteriostatic test of Aeromonas hydrophila and vibrio parahaemolytious, carry out significance analysis, obtain suitable prescription proportioning.
3. choose rheum officinale, golden cypress, the coptis according to parts by weight weighing, after mixing, put into Chinese medicine extracting tank, extract for the first time: add medicinal material weight 10-15 water doubly, extract 100 ℃ of temperature, when being warmed up to 100 ℃, start timing, constant temperature time is 1-2h, and filter will be inputted in original fluid container after medical filtration.Extract for the second time: add medicinal material weight 6-10 water doubly, extracting temperature is 100 ℃, reach 100 ℃ and start timing, constant temperature time is 1-2h, by filter, medical filtration is inputted in original fluid container.In original fluid container, concentrate, being concentrated into medicinal material weight and concentrate weight is to stop for 1: 1 o'clock, obtains concentrated medicament.
4. choose cloves, peppermint, lemongrass, thyme according to parts by weight weighing, respectively or after mixing, put into Chinese medicine extracting tank, add medicinal material weight 8-12 water doubly, when being warmed up to 100 ℃, start timing, boil 4-5 hour, volatile oil is collected in condensation, after the volatile oil of collecting is mixed with appropriate alcohol, put into freezer stand-by.
5. take auxiliary material according to following ratio of weight and number
By glucose, medical stone, dextrin, corn starch adds three-dimensional hybrid fitness machine, mixes 1-2 hour, as carrier.Mixed carrier is placed on disk pelletizing machine.The concentrate that rheum officinale, golden cypress, the coptis are extracted, is sprayed on outward on carrier according to the ratio of parts by weight 2%-8%, uses disk pelletizing machine carrier to be made to the Chinese herbal medicine particle of 2mm-8mm.
6. the Chinese herbal medicine particle making is put into 60 ℃ of-70 ℃ of baking ovens and dried 4-8 hour (moisture is less than 8%).Use spherical mixer, the volatile oil that cloves, peppermint, lemongrass, thyme are extracted is sprayed on particle surface outward according to the ratio of parts by weight 2%-6%, packs into fast in the aluminium foil bag of 500 grams/bag, presses bag sealing, to obtain final product.
Embodiment
By more specific description the present invention, make technical matters of the present invention and using method be easier to understand by the following example.
Embodiment 1
1. filter out the traditional Chinese medicine that can suppress Aeromonas hydrophila and vibrio parahaemolytious, rheum officinale, golden cypress, the coptis, cloves, peppermint, lemongrass, thyme.
2. by the multiple medicinal material filtering out, by orthogonal experiment, carry out the bacteriostatic test of Aeromonas hydrophila and vibrio parahaemolytious, carry out significance analysis, obtain suitable prescription proportioning.
3. take by weight:
Rheum officinale 300g
Golden cypress 200g
Coptis 100g
After medicinal material is mixed, put into Chinese medicine extracting tank, extract for the first time: add 6L water, extract 100 ℃ of temperature, wait while being warmed up to 100 ℃, start timing, constant temperature time is 1.5h, and 4 layers of filtered through gauze are poured in fluid reservoir.Extract for the second time: add the water of 4.8L, extracting temperature is 100 ℃, reach 100 ℃ and start timing, constant temperature time is 1.5h, and 4 layers of filtered through gauze are poured in fluid reservoir.In fluid reservoir, heat and concentrate, in the time that concentrate is 600ml, stop.
4. take according to weight,
After mixing, put into Chinese medicine extracting tank, add 4L water, wait while being warmed up to 100 ℃, start timing, boil 4-5 hour, volatile oil is collected in condensation, after the volatile oil 1L collecting is mixed with the alcohol of 1L, puts into freezer stand-by.
5. take according to following weight:
Glucose, medical stone, dextrin, corn starch are added to three-dimensional hybrid fitness machine, mix 1 hour, as carrier.Mixed carrier is placed on disk pelletizing machine.The concentrate 600ml that rheum officinale, golden cypress, the coptis are extracted is sprayed on outward on carrier, uses disk pelletizing machine carrier to be made to the Chinese herbal medicine particle of 4mm.
6. the Chinese herbal medicine particle making is put into 60 ℃ of baking ovens and dried 7 hours (moisture is 6.38%).Use spherical mixer, the volatile oil 1.2L that cloves, peppermint, lemongrass, thyme are extracted is sprayed on the particle surface of having dried outward, packs into fast in the aluminium foil bag of 500 grams/bag, presses bag sealing, obtains this product.
Embodiment 2: the fungistatic effect of Chinese herbal medicine particle of the present invention is investigated
1 test objective: detect the fungistatic effect of Chinese herbal medicine particle of the present invention for Aeromonas hydrophila and vibrio parahaemolytious.
2, instrument and medicine
2.1 test apparatuses: clean bench, autoclave, climatic cabinate, electronic analytical balance
2.2 test strains: Aeromonas hydrophila, vibrio parahaemolytious.Purchased from China Microbial Culture Preservation Commission's common micro-organisms center.
2.3 reagent: sodium chloride (analyzing pure), beef extract (biochemical level), peptone (biochemical level), Chinese herbal medicine particle of the present invention is provided by Beijing Centre Biology Co., Ltd..
3 test methods:
The preparation of 3.1 bacteria suspensions: Aeromonas hydrophila is inoculated on beef-protein medium, and vibrio parahaemolytious bacterial classification is inoculated in 3% high salt beef-protein medium, cultivates 16-24 hour in 30 ℃.Being diluted to concentration is 10
5-10
6the bacteria suspension (aseptic 8.5 ‰ physiological saline gradient dilutions) of cfu/ml.
Beef-protein medium formula
3.2 test operation programs:
Be divided into 8 groups by 2 kinds for examination bacterium liquid, every group of 2 repetitions, be respectively blank group, positive controls (10% Betagen Solution 0.5ppm), 0.5ppm groups of grains of the present invention and 1ppm groups of grains of the present invention (specifically grouping situation is in table 1), add after drug effect 1h, all shake up, sampling, carries out count plate respectively, calculates bacteriostasis rate.
The grouping of table 1 bacteriostatic test
Numbering | Aeromonas hydrophila bacterium liquid Chinese traditional medicine adds concentration | Numbering | Vibrio parahaemolytious bacterium liquid Chinese traditional medicine adds concentration |
1 group | 0 (blank group) | 5 groups | 0 (blank group) |
2 groups | 10% povidone iodine 0.5ppm | 6 groups | 10% povidone iodine 0.5ppm |
3 groups | Particle 0.5ppm of the present invention | 7 groups | Particle 0.5ppm of the present invention |
4 groups | Particle 1ppm of the present invention | 8 groups | Particle 1ppm of the present invention |
4, experimental result and discussion:
Table 2 bacteriostatic test result
5, conclusion: after particle of the present invention uses 1 hour for Aeromonas hydrophila, 0.5ppm dosage group bacteriostasis rate is 57.83%, 1ppm dosage group bacteriostasis rate is 86.75%, all higher than the bacteriostasis rate (40.96%) of 10% Betagen Solution 0.5ppm dosage group.After particle of the present invention uses 1 hour for vibrio parahaemolytious, 0.5ppm dosage group bacteriostasis rate is that 45.71%, 1ppm dosage group bacteriostasis rate is 77.14%, all higher than 10% povidone iodine bacteriostasis rate (34.29%).Therefore, be 200~300 grams/acre of rice for the recommendation dosage of freshwater aquiculture, the recommended dose of mariculture is 300~500 grams/acre of rice.
Should be understood that, those of ordinary skills can improve or change according to this explanation, and these improvement and conversion all should belong to the protection domain of claims of the present invention.