CN111195285A - Method for preparing antibacterial agent by using attapulgite to support Chinese herbal medicine - Google Patents

Method for preparing antibacterial agent by using attapulgite to support Chinese herbal medicine Download PDF

Info

Publication number
CN111195285A
CN111195285A CN202010084669.9A CN202010084669A CN111195285A CN 111195285 A CN111195285 A CN 111195285A CN 202010084669 A CN202010084669 A CN 202010084669A CN 111195285 A CN111195285 A CN 111195285A
Authority
CN
China
Prior art keywords
attapulgite
chinese herbal
herbal medicine
antibacterial agent
preparing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010084669.9A
Other languages
Chinese (zh)
Inventor
王爱勤
惠爱平
黄正君
杨芳芳
康玉茹
许江
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
JIANGSU SINITIC BIOLOGICAL TECHNOLOGY Co.,Ltd.
Lanzhou Institute of Chemical Physics LICP of CAS
Original Assignee
Jiangsu Sinitic Biological Technology Co ltd
Xuyi R & D Center For Application Of Attapulgite Clay Lanzhou Institute Of Chemical Physics Chinese Academy Of Sciences
Lanzhou Institute of Chemical Physics LICP of CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangsu Sinitic Biological Technology Co ltd, Xuyi R & D Center For Application Of Attapulgite Clay Lanzhou Institute Of Chemical Physics Chinese Academy Of Sciences, Lanzhou Institute of Chemical Physics LICP of CAS filed Critical Jiangsu Sinitic Biological Technology Co ltd
Priority to CN202010084669.9A priority Critical patent/CN111195285A/en
Publication of CN111195285A publication Critical patent/CN111195285A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/539—Scutellaria (skullcap)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/19—Acanthaceae (Acanthus family)
    • A61K36/195—Strobilanthes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A61K36/315—Isatis, e.g. Dyer's woad
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/35—Caprifoliaceae (Honeysuckle family)
    • A61K36/355—Lonicera (honeysuckle)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/51—Gentianaceae (Gentian family)
    • A61K36/515—Gentiana
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/14—Esters of carboxylic acids, e.g. fatty acid monoglycerides, medium-chain triglycerides, parabens or PEG fatty acid esters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medical Informatics (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Communicable Diseases (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Neurosurgery (AREA)
  • Physiology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention discloses a method for preparing an antibacterial agent by utilizing attapulgite supported Chinese herbal medicines, which comprises the steps of spraying active components of the Chinese herbal medicines on the surface of attapulgite powder under stirring, sequentially spraying liquid fatty glyceride and quaternized chitosan oligosaccharide aqueous solution after mechanical grinding, uniformly stirring, and then carrying out roll-rolling, drying, crushing and packaging. According to the invention, the Chinese herbal medicine active components with antibacterial activity reside in the pore structure of the attapulgite through mechanochemical action, the fatty glyceride is utilized to form primary encapsulation on the surface of the attapulgite, and the quaternary chitosan oligosaccharide is utilized to realize secondary encapsulation, so that the obtained attapulgite immobilized Chinese herbal medicine antibacterial agent has good thermal stability, and the defect of poor thermal stability of the Chinese herbal medicine antibacterial active components is overcome.

Description

Method for preparing antibacterial agent by using attapulgite to support Chinese herbal medicine
Technical Field
The invention relates to a preparation method of an antibacterial agent, in particular to a method for preparing an antibacterial agent by utilizing attapulgite supported Chinese herbal medicines, which is mainly used for replacing antibiotics in the process of livestock and poultry breeding and belongs to the field of animal feed.
Background
Since the discovery of antibiotics, the antibiotics play a positive role in promoting the animal production performance and intensive animal breeding. However, with long-term and excessive use, a chain of 'feed antibiotics use-animal drug-resistant bacteria production-food chain transfer-human drug-resistant pathogenic bacteria infection' has been formed, and the chain poses a potential threat to human health. 7, 9.2019, 194 th bulletin board is officially released by rural parts of agriculture, a drug feed additive quit plan and related management policies are published, and 'comprehensive resistance forbidding' in 2020 is definitely proposed, which marks that green breeding becomes a great trend, and the research and development of novel safe and efficient feed antibiotic substitute products are imminent.
In recent years, Chinese herbal medicine extracts have attracted high attention because they do not produce bacterial resistance, and related products have begun to be used in animal feeds. However, the molecular structure of the Chinese herbal medicine extract is sensitive to the environment and is easy to inactivate, and the loss of active components in the feed processing process causes poor stability of product batches, so that the application of the Chinese herbal medicine extract is limited to a certain extent.
Attapulgite is a natural one-dimensional magnesium-aluminum-rich silicate clay mineral, has a nano rod-shaped crystal structure, nano pores and surface active groups, and is widely applied in various fields. Researches show that attapulgite is added into the feed to adsorb toxic and harmful substances such as pathogenic bacteria, heavy metals, mycotoxin and the like in the intestinal tract, so that the nutrient utilization rate of the feed is improved, the intestinal health of animals is protected, and the production performance of the animals is improved (ZL 201510285208.7; agricultural science and technology and equipment, 2019, (02): 69-70). Attapulgite is listed in feed additives, but the combination of attapulgite and Chinese herbal medicine extract, the primary encapsulation of fatty glyceride on the surface of attapulgite, and the secondary encapsulation of fatty glyceride by quaternized chitosan oligosaccharide have been reported to solve the technical problem of easy oxidation of Chinese herbal medicine extract in feed.
Disclosure of Invention
The invention aims to overcome the defects of easy inactivation of Chinese herbal medicine extracts and the like, and provides a preparation method of a botanical antibacterial preparation with good thermal stability.
The principle of the invention is that the adsorption function of attapulgite pore canals is utilized, the Chinese herbal medicine extract is retained in the attapulgite pore canals through mechanochemical action, the fatty glyceride is utilized to form primary encapsulation on the surface of the attapulgite, and the quaternary chitosan oligosaccharide is utilized to realize secondary encapsulation, thereby solving the technical problem that the Chinese herbal medicine extract is easy to oxidize when being applied to feed.
Preparation of Chinese herbal medicine antibacterial preparation
The preparation method of the Chinese herbal medicine antibacterial preparation comprises the steps of spraying the active ingredients of the Chinese herbal medicines on the surface of the attapulgite powder under stirring, sequentially spraying liquid fatty glyceride and quaternized chitosan oligosaccharide aqueous solution after mechanical grinding, stirring for 30 minutes, and then carrying out roll-rolling, drying, crushing and packaging.
The attapulgite is subjected to sulfuric acid acidification treatment and sand removal, wherein the mass fraction of the sulfuric acid acidification treatment is 2-3% relative to that of the attapulgite, so that a product with the purity of more than or equal to 90% is obtained.
The Chinese herbal medicine active components are at least one of licorice extract, isatis root extract, astragalus extract, honeysuckle extract and gentian extract; wherein each extract is a combined solution of crushed Chinese herbal medicines and water extracts for 3 times. The dosage of the Chinese herbal medicine active component is 5-10% of the mass of the attapulgite.
The fatty acid glyceride is at least one of lauric acid monoglyceride, mono-capric acid glyceride, mono-myristic acid glyceride, mono-lactic acid glyceride, di-lactic acid glyceride and tri-lactic acid glyceride; the dosage of the fatty glyceride is 10-20% of the mass of the attapulgite.
The quaternized chitosan oligosaccharide aqueous solution is one of an N-trimethyl chitosan oligosaccharide quaternary ammonium salt aqueous solution, an N-alkylated chitosan oligosaccharide quaternary ammonium salt aqueous solution, a carboxymethyl hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution, an N-hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution and an O-hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution. The dosage of the quaternized chitosan oligosaccharide is 0.5-2.5% of the mass of the attapulgite.
The mechanical grinding is ball grinding, the ball-material ratio is 1:10, and the grinding time is 30 minutes.
The pair roller is formed by extruding a mixture by using a pair roller machine, and the thickness of the pair roller sheet is 2-5 mm.
Second, evaluation of antibacterial property of Chinese herbal medicine active component preparation
And (4) evaluating the antibacterial performance by adopting a minimum inhibitory concentration method. Wherein, an experimental group, a negative control group, a positive control group and a blank control group are arranged.
(1) Preparation of standard strains: escherichia coli standard strain ATCC 25922, staphylococcus aureus standard strain ATCC 25923. Taking escherichia coli ATCC 25922 and staphylococcus aureus ATCC 25923 stored at the temperature of-20 ℃, unfreezing the escherichia coli and inoculating the escherichia coli and the staphylococcus aureus ATCC 25923 to an LB broth culture medium, incubating the culture medium in a 35 ℃ shaking box for 12 hours at a shaking frequency of 160 r/min, reviving the strains, transferring the strains to a nutrient agar culture medium again, placing the culture medium in a constant temperature incubator for incubation at the temperature of 37 ℃, and after 3 hours, carrying out bacterial passage and entering a log phase. Selecting single colony of standard strain entering logarithmic phase to prepare bacterial suspension, adjusting turbidity of bacterial suspension to 0.5 McFarland standard turbidity, and sequentially diluting with PBS buffer solution 1:10 to make its bacterial content 107CFU/mL、106CFU/mL、105CFU/mL、104CFU/mL。
(2) Preparing agar plates of samples to be detected with different concentrations: escherichia coli groups were 1mg/mL, 0.5mg/mL, 0.25mg/mL, 0.1mg/mL, and Staphylococcus aureus groups were 0.5mg/mL, 0.25mg/mL, 0.1mg/mL, 0.05 mg/mL.
(3) Dibbling: pipetting 1. mu.L of the above suspension (containing about 1X 10 cells) with a pipette4CFU/mL, spotted on agar plate dishes containing the samples to be tested, each plate spotted in 3 different locations, and 2 replicates were performed. All plates were transferred to a constant temperature incubator for incubation at 37 ℃ for 24 hours, and then the growth of bacteria was observed.
Fig. 1 is a side view digital photograph of the antibacterial agent (a) prepared according to the present invention, which is formed as a suspension (b) after the antibacterial agent is dissolved in water, and a top view digital photograph (c) of the suspension formed as the antibacterial agent is dissolved in water. As can be seen from fig. 1, the antibacterial agent forms a uniform suspension after being dissolved in water, and has good water solubility and dispersibility.
FIG. 2 shows the results of the minimum inhibitory concentration test of the antibacterial agent prepared according to the present invention against Escherichia coli (a) a blank, (b) 1mg/mL, (c) 0.5mg/mL, (d) 0.25mg/mL, (e) 0.1mg/mL and Staphylococcus aureus (f) a blank, (g) 0.5mg/mL, (h) 0.25mg/mL, (i) 0.1mg/mL, (j) 0.05 mg/mL. The result shows that the minimum inhibitory concentration of the antibacterial preparation prepared by the invention to Escherichia coli and Staphylococcus aureus is 0.25mg/mL and 0.1mg/mL respectively, and the antibacterial preparation shows strong antibacterial activity to gram-negative bacteria and gram-positive bacteria.
In summary, the present invention has the following advantages over the prior art:
1. according to the invention, the Chinese herbal medicine active components with antibacterial activity reside in an attapulgite pore structure through mechanochemical action, fatty glyceride is utilized to form primary encapsulation on the surface of attapulgite, secondary encapsulation is realized through quaternized chitosan oligosaccharide, the obtained attapulgite immobilized Chinese herbal medicine antibacterial agent has good thermal stability, the defect of poor thermal stability of the Chinese herbal medicine antibacterial active components is overcome, and on the basis of effectively realizing encapsulation of the Chinese herbal medicine active components, the obtained powder preparation has good water solubility and thermal stability, and can be used for processing requirements of feeds at different temperatures;
2. the antibacterial preparation prepared by the invention has broad-spectrum antibacterial activity, shows strong antibacterial performance to both gram-positive bacteria and gram-negative bacteria, and can replace antibiotics in the livestock and poultry breeding process;
3. the invention has simple process and is easy to realize industrial production.
Drawings
Fig. 1 is a digital photograph showing a suspension of the antibacterial agent prepared according to the present invention dissolved in water.
Fig. 2 is a result of a minimum inhibitory concentration test of the antibacterial agent prepared according to the present invention.
Detailed Description
The method for preparing the antibacterial preparation of active ingredients of herbal medicines according to the present invention will be described in detail with reference to the following examples.
Example 1
Spraying 5% of radix Scutellariae extract relative to attapulgite mass fraction on the surface of attapulgite under stirring, and grinding in a ball mill at a ball-to-material ratio of 1:10 for 30 min. Placing the mixed material in a stirring tank, spraying hot-melt lauric acid monoglyceride accounting for 20% of the mass fraction of the attapulgite, then spraying N-trimethyl chitosan oligosaccharide quaternary ammonium salt aqueous solution accounting for 1% of the mass fraction of the attapulgite into the mixture, continuously stirring for 30min after spraying is finished, then placing the mixture in a pair roller machine for pair roller treatment, and drying, crushing and packaging the material to obtain the product.
Example 2
Spraying 7.5% of radix astragali extract relative to attapulgite on the surface of attapulgite under stirring, and grinding in a ball mill at a ball-to-material ratio of 1:10 for 30 min. Placing the mixed material in a stirring tank, spraying hot-melt monodecane lauric glyceride accounting for 15% of the mass fraction of the attapulgite, then spraying an N-alkylated chitosan oligosaccharide quaternary ammonium salt aqueous solution accounting for 1.5% of the mass fraction of the attapulgite into the mixture, continuously stirring for 30min after the spraying is finished, then placing the mixture in a pair roller machine for pair roller treatment, and drying, crushing and packaging the material to obtain the product.
Example 3
Spraying 10% of radix astragali extract relative to attapulgite mass fraction on the surface of attapulgite under stirring, and grinding in a ball mill at a ball-to-material ratio of 1:10 for 30 min. Placing the mixed material in a stirring tank, spraying 10% of monomyristyl monoglyceride and 10% of lauric acid monoglyceride relative to the mass fraction of attapulgite, then spraying 2% of carboxymethyl hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution relative to the mass fraction of the attapulgite into the mixture, continuously stirring for 30min after the spraying is finished, then placing the mixture in a pair roller machine for roller treatment, and drying, crushing and packaging the material to obtain the product.
Example 4
Spraying a mixture of 2.5 percent of honeysuckle extract and 2.5 percent of gentian extract on the surface of the attapulgite under stirring, and then placing the mixture in a ball mill for grinding for 30 minutes at a ball-to-material ratio of 1: 10. Placing the mixed material in a stirring tank, spraying 2.5% of glycerol mono-lactate and 7.5% of glycerol monolaurate relative to the mass fraction of attapulgite, then spraying 1.5% of carboxymethyl hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution relative to the mass fraction of the attapulgite into the mixture, continuously stirring for 30min after spraying is finished, then placing the mixture in a pair roller machine for roller treatment, and drying, crushing and packaging the material to obtain the product.
Example 5
Spraying a mixture of the isatis root extract and the liquorice root extract with the mass fraction of 2.5 percent relative to the attapulgite on the surface of the attapulgite under stirring, and then placing the attapulgite in a ball mill for grinding for 30 minutes at a ball-to-material ratio of 1: 10. Placing the mixed material in a stirring tank, spraying 5% of monocaprylic acid glyceride and 5% of lauric acid monoglyceride relative to the mass fraction of attapulgite, then spraying 2% of carboxymethyl hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution relative to the mass fraction of the attapulgite into the mixture, continuously stirring for 30min after the spraying is finished, then placing the mixture in a pair roller machine for pair roller treatment, and drying, crushing and packaging the material to obtain the product.
Example 6
Spraying a mixture of honeysuckle extract 3 wt%, astragalus extract 1 wt% and licorice extract 1 wt% on the surface of the attapulgite under stirring, and grinding in a ball mill at a ball-to-material ratio of 1:10 for 30 min. Placing the mixed material in a stirring tank, spraying 5% of triglyceride and 10% of lauric acid monoglyceride relative to the mass fraction of attapulgite, then spraying 2.5% of N-hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution relative to the mass fraction of the attapulgite into the mixture, continuously stirring for 30min after the spraying is finished, then placing the mixture in a pair roller machine for roller treatment, and drying, crushing and packaging the material to obtain the product.
Example 7
Spraying a mixture of 1% of liquorice extract, 2.5% of baicalin and 2.5% of isatis root extract relative to the mass fraction of the attapulgite on the surface of the attapulgite under stirring, and then placing the attapulgite in a ball mill for grinding for 30 minutes at a ball-to-material ratio of 1: 10. Placing the materials in a stirring tank, spraying 2.5% of monocaprylic acid glyceride, 2.5% of diglyceride and 5% of monolaurin relative to the mass fraction of attapulgite, then spraying 0.5% of O-hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution relative to the mass fraction of the attapulgite in the mixture, continuously stirring for 30min after spraying is finished, then placing the mixture in a pair roller machine for roller treatment, and drying, crushing and packaging the materials to obtain the product.
In the embodiments, the attapulgite is subjected to acidification treatment and sand removal by using sulfuric acid with the mass fraction of 2-3% relative to the attapulgite to obtain a product with the purity of more than or equal to 90%; each extract is a combined solution of crushed Chinese herbal medicines and water extracts for 3 times.

Claims (10)

1. A method for preparing antibacterial agent from attapulgite supported Chinese herbal medicine comprises spraying active components of Chinese herbal medicine onto the surface of attapulgite powder under stirring, mechanically grinding, spraying liquid fatty glyceride and quaternized chitosan oligosaccharide aqueous solution in sequence, stirring, roller-drying, crushing, and packaging.
2. The method for preparing the antibacterial agent by using the attapulgite-immobilized Chinese herbal medicine as claimed in claim 1, wherein the method comprises the following steps: the attapulgite is subjected to sulfuric acid acidification treatment and sand removal, wherein the mass fraction of the sulfuric acid acidification treatment is 2-3% relative to that of the attapulgite, so that a product with the purity of more than or equal to 90% is obtained.
3. The method for preparing the antibacterial agent by using the attapulgite-immobilized Chinese herbal medicine as claimed in claim 1, wherein the method comprises the following steps: the Chinese herbal medicine active component is at least one of licorice extract, isatis root extract, astragalus extract, honeysuckle extract and gentian extract.
4. The method for preparing the antibacterial agent by using the attapulgite-immobilized Chinese herbal medicine as claimed in claim 3, wherein the method comprises the following steps: the dosage of the active components of the Chinese herbal medicines is 5-10% of the mass of the attapulgite.
5. The method for preparing the antibacterial agent by using the attapulgite-immobilized Chinese herbal medicine as claimed in claim 3, wherein the method comprises the following steps: the Chinese herbal medicine extract is a combined solution of three water extracts of Chinese herbal medicines after being crushed.
6. The method for preparing the antibacterial agent by using the attapulgite-immobilized Chinese herbal medicine as claimed in claim 1, wherein the method comprises the following steps: the fatty acid glyceride is at least one of lauric acid monoglyceride, mono-capric acid glyceride, mono-myristic acid glyceride, mono-lactic acid glyceride, di-lactic acid glyceride, and tri-lactic acid glyceride.
7. The method for preparing the antibacterial agent by using the attapulgite-immobilized Chinese herbal medicine as claimed in claim 6, wherein the method comprises the following steps: the dosage of the fatty glyceride is 10-20% of the mass of the attapulgite.
8. The method for preparing attapulgite-supported Chinese herbal medicine antibacterial agent according to claim 1, wherein the method comprises the following steps: the quaternized chitosan oligosaccharide aqueous solution is one of an N-trimethyl chitosan oligosaccharide quaternary ammonium salt aqueous solution, an N-alkylated chitosan oligosaccharide quaternary ammonium salt aqueous solution, a carboxymethyl hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution, an N-hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution and an O-hydroxypropyl trimethyl ammonium chloride chitosan oligosaccharide aqueous solution.
9. The method for preparing the antibacterial agent by using the attapulgite-immobilized Chinese herbal medicine as claimed in claim 8, wherein the method comprises the following steps: the dosage of the quaternized chitosan oligosaccharide is 0.5-2.5% of the mass of the attapulgite.
10. The method for preparing the antibacterial agent by using the attapulgite-immobilized Chinese herbal medicine as claimed in claim 1, wherein the method comprises the following steps: the mechanical grinding is ball mill grinding, the ball-material ratio is 1:10, and the grinding time is 30 minutes.
CN202010084669.9A 2020-02-10 2020-02-10 Method for preparing antibacterial agent by using attapulgite to support Chinese herbal medicine Pending CN111195285A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010084669.9A CN111195285A (en) 2020-02-10 2020-02-10 Method for preparing antibacterial agent by using attapulgite to support Chinese herbal medicine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010084669.9A CN111195285A (en) 2020-02-10 2020-02-10 Method for preparing antibacterial agent by using attapulgite to support Chinese herbal medicine

Publications (1)

Publication Number Publication Date
CN111195285A true CN111195285A (en) 2020-05-26

Family

ID=70742406

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010084669.9A Pending CN111195285A (en) 2020-02-10 2020-02-10 Method for preparing antibacterial agent by using attapulgite to support Chinese herbal medicine

Country Status (1)

Country Link
CN (1) CN111195285A (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112715575A (en) * 2021-03-03 2021-04-30 中国科学院兰州化学物理研究所 Attapulgite-fulvic acid hybrid antibacterial material and preparation method thereof
CN112741233A (en) * 2021-03-03 2021-05-04 中国科学院兰州化学物理研究所 Method for preparing suspended granular fish feed by using sea buckthorn fruit powder
CN112841414A (en) * 2021-03-03 2021-05-28 中国科学院兰州化学物理研究所 Preparation method of sea buckthorn inclusion compound with antibacterial and antioxidant functions
CN112868934A (en) * 2021-01-19 2021-06-01 山东省汇富农牧发展有限公司 Nano montmorillonite meat rabbit feed additive compound for detoxification and diarrhea prevention
WO2021160135A1 (en) * 2020-02-10 2021-08-19 中国科学院兰州化学物理研究所 Method for preparing natural antibacterial agent by using attapulgite pore channels to immobilize plant essential oil
WO2021203833A1 (en) * 2020-04-10 2021-10-14 中国科学院兰州化学物理研究所 Method for preparation of attapulgite-based ph-responsive antibacterial material
CN113545420A (en) * 2021-07-28 2021-10-26 中国科学院兰州化学物理研究所 Preparation and application of codonopsis pilosula polysaccharide @ attapulgite gel antibacterial material
CN114540045A (en) * 2022-01-26 2022-05-27 中国科学院兰州化学物理研究所 Soapberry modified attapulgite and preparation method of O/W type Pickering emulsion of plant essential oil of soapberry modified attapulgite
CN114533758A (en) * 2022-01-26 2022-05-27 中国科学院兰州化学物理研究所 Method for preparing selenium/attapulgite composite antibacterial material by using aloe extract
CN114651916A (en) * 2022-04-22 2022-06-24 中国科学院兰州化学物理研究所 Functional attapulgite-based aquatic feed adhesive

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104888691A (en) * 2015-05-29 2015-09-09 中国科学院兰州化学物理研究所盱眙凹土应用技术研发中心 Preparation method of antibacterial attapulgite zearalenone adsorbent
US20170196812A1 (en) * 2014-10-01 2017-07-13 Cool Planet Energy Systems, Inc. Biochars for use with animals
CN106962427A (en) * 2017-04-28 2017-07-21 明光市飞洲新材料有限公司 A kind of concave convex rod mosquito repellent powder and preparation method thereof
CN107320488A (en) * 2017-07-06 2017-11-07 中国科学院兰州化学物理研究所盱眙凹土应用技术研发中心 A kind of preparation method of chitosan oligosaccharide/ZnO/ palygorskite nano complex antimicrobials
CN109498645A (en) * 2018-11-28 2019-03-22 中国科学院兰州化学物理研究所盱眙凹土应用技术研发中心 Charge regulation cooperates with the high-pressure homogeneous method for preparing attapulgite cationic antibacterial agent

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170196812A1 (en) * 2014-10-01 2017-07-13 Cool Planet Energy Systems, Inc. Biochars for use with animals
CN104888691A (en) * 2015-05-29 2015-09-09 中国科学院兰州化学物理研究所盱眙凹土应用技术研发中心 Preparation method of antibacterial attapulgite zearalenone adsorbent
CN106962427A (en) * 2017-04-28 2017-07-21 明光市飞洲新材料有限公司 A kind of concave convex rod mosquito repellent powder and preparation method thereof
CN107320488A (en) * 2017-07-06 2017-11-07 中国科学院兰州化学物理研究所盱眙凹土应用技术研发中心 A kind of preparation method of chitosan oligosaccharide/ZnO/ palygorskite nano complex antimicrobials
CN109498645A (en) * 2018-11-28 2019-03-22 中国科学院兰州化学物理研究所盱眙凹土应用技术研发中心 Charge regulation cooperates with the high-pressure homogeneous method for preparing attapulgite cationic antibacterial agent

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
HUI, AIPING, ET AL.: "Hydrothermal fabrication of spindle-shaped ZnO/palygorskite nanocomposites using nonionic surfactant for enhancement of antibacterial activity", 《NANOMATERIALS》 *
任觉世: "《工业矿产资源开发利用手册》", 31 January 1993, 武汉工业大学出版社 *
唐洁等: "季铵盐有机化改性伊蒙混层黏土的悬浮性能", 《硅酸盐通报》 *
张一宾: "《工业防霉剂》", 28 February 2001, 中国物资出版社 *
汪多仁: "《绿色增塑剂》", 31 October 2011, 科学技术文献出版社 *
王冲等: "抗菌矿物材料及其研究现状", 《中国非金属矿工业导刊》 *
谢明全等: "凹凸棒石对中药水煎液中芍药苷和阿魏酸的吸附影响", 《中国医院药学杂志》 *
齐贵亮: "《塑料改性配方工艺速查360例》", 31 January 2016, 印刷工业出版社 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021160135A1 (en) * 2020-02-10 2021-08-19 中国科学院兰州化学物理研究所 Method for preparing natural antibacterial agent by using attapulgite pore channels to immobilize plant essential oil
US11700849B2 (en) 2020-04-10 2023-07-18 Lanzhou Institute Of Chemical Physics, Chinese Academy Of Sciences Method for preparation of attapulgite-based pH-responsive antibacterial material
WO2021203833A1 (en) * 2020-04-10 2021-10-14 中国科学院兰州化学物理研究所 Method for preparation of attapulgite-based ph-responsive antibacterial material
CN112868934A (en) * 2021-01-19 2021-06-01 山东省汇富农牧发展有限公司 Nano montmorillonite meat rabbit feed additive compound for detoxification and diarrhea prevention
CN112741233A (en) * 2021-03-03 2021-05-04 中国科学院兰州化学物理研究所 Method for preparing suspended granular fish feed by using sea buckthorn fruit powder
CN112841414A (en) * 2021-03-03 2021-05-28 中国科学院兰州化学物理研究所 Preparation method of sea buckthorn inclusion compound with antibacterial and antioxidant functions
CN112715575A (en) * 2021-03-03 2021-04-30 中国科学院兰州化学物理研究所 Attapulgite-fulvic acid hybrid antibacterial material and preparation method thereof
CN112741233B (en) * 2021-03-03 2022-04-29 中国科学院兰州化学物理研究所 Method for preparing suspended granular fish feed by using sea buckthorn fruit powder
CN113545420A (en) * 2021-07-28 2021-10-26 中国科学院兰州化学物理研究所 Preparation and application of codonopsis pilosula polysaccharide @ attapulgite gel antibacterial material
CN114533758A (en) * 2022-01-26 2022-05-27 中国科学院兰州化学物理研究所 Method for preparing selenium/attapulgite composite antibacterial material by using aloe extract
CN114540045B (en) * 2022-01-26 2022-10-28 中国科学院兰州化学物理研究所 Preparation method of plant essential oil O/W type Pickering emulsion
CN114533758B (en) * 2022-01-26 2022-11-11 中国科学院兰州化学物理研究所 Method for preparing selenium/attapulgite composite antibacterial material by using aloe extract
CN114540045A (en) * 2022-01-26 2022-05-27 中国科学院兰州化学物理研究所 Soapberry modified attapulgite and preparation method of O/W type Pickering emulsion of plant essential oil of soapberry modified attapulgite
CN114651916A (en) * 2022-04-22 2022-06-24 中国科学院兰州化学物理研究所 Functional attapulgite-based aquatic feed adhesive

Similar Documents

Publication Publication Date Title
CN111195285A (en) Method for preparing antibacterial agent by using attapulgite to support Chinese herbal medicine
CN111328918B (en) Preparation method of attapulgite-based pH response antibacterial material
CN111034861A (en) Method for preparing oil-in-water emulsion coated plant essential oil by using lauric acid monoglyceride
CN104855684B (en) A kind of antibacterial Chinese medicine microbial fermentation preparation and its preparation method and application
CN111011612A (en) Method for preparing natural antibacterial agent by using attapulgite pore channel immobilized plant essential oil
CN103814967A (en) Chinese herbal medicine granular preparation used for aquatic product disinfection and preparing method thereof
CN112358997B (en) Bacterial strain with biocontrol function on tobacco brown spot, fermentation method of bacterial strain and tobacco brown spot prevention and control compound agent
CN112136965B (en) Immunity-enhancing and growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof
CN105858909A (en) Microbial herbal composite preparation for aquiculture and preparation method thereof
CN113616715B (en) Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora thereof
KR100951273B1 (en) Feed additive containing an extract of bamboo and microorganism cultivate,for prevention of Porcinediarrhea caused by enterotoxigenic enterobacteria
CN105267957B (en) One boar biological antidiarrheic and its preparation method and application
CN110973357A (en) Slow-release type compound acidifier and preparation method thereof
CN107047972B (en) Functional feed added with water bloom blue algae extract
CN105166397B (en) Brevibacillus brevis microecological preparation
KR101420836B1 (en) Livestock feed additive with the culture medium of Latobacillus plantarum
EP1894995B1 (en) Irilis biopreparation based on bacillus-strain bacteria, bacillus subtilis and bacillus licheniformis contained therein
CN110157648B (en) Microbial fertilizer utilizing waste resources in pig raising industry and preparation method thereof
CN111548965A (en) Donkey-derived bacillus pumilus and application thereof in preparation of medicine for treating diarrhea of donkey colt
CN113424900A (en) Preparation method, product and application of probiotic feed additive
CN113040278A (en) Montmorillonite modified material and preparation method and application thereof
CN111821322A (en) Poultry micro-ecological oral preparation capable of replacing antibiotics and application thereof
CN109105666A (en) A kind of fish dedicated liquid acidulant and preparation method thereof
CN116162557B (en) A strain of Saccharomyces cerevisiae, chinese medicinal microecological preparation for preventing and treating ruminant diarrhea, and its preparation method
CN117796405A (en) Biopesticide for preventing and treating tobacco wildfire

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20210806

Address after: 730000 No. 18 Tianshui Middle Road, Chengguan District, Gansu, Lanzhou

Applicant after: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences

Applicant after: JIANGSU SINITIC BIOLOGICAL TECHNOLOGY Co.,Ltd.

Address before: 211700 No. 1, Xianglan Avenue, Aotu science and Technology Industrial Park, Xuyi county, Huai'an City, Jiangsu Province

Applicant before: XUYI R & D CENTER FOR APPLICATION OF ATTAPULGITE CLAY, LANZHOU INSTITUTE OF CHEMICAL PHYSICS CHINESE ACADEMY OF SCIENCES

Applicant before: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences

Applicant before: JIANGSU SINITIC BIOLOGICAL TECHNOLOGY Co.,Ltd.

TA01 Transfer of patent application right
RJ01 Rejection of invention patent application after publication

Application publication date: 20200526

RJ01 Rejection of invention patent application after publication