CN103814967B - Chinese herbal medicine granular preparation of a kind of aquatic products sterilization use and preparation method thereof - Google Patents
Chinese herbal medicine granular preparation of a kind of aquatic products sterilization use and preparation method thereof Download PDFInfo
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- CN103814967B CN103814967B CN201410072837.7A CN201410072837A CN103814967B CN 103814967 B CN103814967 B CN 103814967B CN 201410072837 A CN201410072837 A CN 201410072837A CN 103814967 B CN103814967 B CN 103814967B
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Abstract
The invention discloses Chinese herbal medicine granular preparation of a kind of aquatic products sterilization use and preparation method thereof. A kind of composition of the present invention is made up of rheum officinale, golden cypress, the coptis, cloves, peppermint, lemongrass, thyme. It has solved current breeding water body bottom sterilization difficulty, and conventional chemical disinfectant is residual serious, the problems such as contaminated environment. The present invention produces simply, and actual operation is strong, and bacterium, fungi are all had to good killing action. Environmental protection no drug residue is a kind of pure Chinese medicinal preparation.
Description
(1) technical field
The present invention relates to a kind of Chinese medicine composition with disinfective action, be specifically related to a kind of aquatic products substrate antibacterialGrain preparation and preparation method thereof.
(2) background technology
Along with fish culture scale expands increasingly, disease problem is also day by day serious, causes serious danger to China's aquacultureEvil, the economic loss causing is every year huge. Because fish live in water body environment complicated and changeable, and fish disease for many yearsSick mostly be to use antibiotic and chemotherapy, consequently not only kill pathogen, the while has also been killed a large amount of usefulBacterium, makes in water body environment normal flora out of proportion, causes ecological deterioration. Along with endurance strain is on the increase, antibiotic is indiscriminatePollution with, the caused medicament residue of chemicals to water body environment and day by day serious to the harm of cultured fishes health, rightThe health of fish causes serious threat.
As the saying goes " breed fish and first support water ", in recent years, the application in aquaculture of substrate modifier and water conditioner is non-Normal general, especially in the cultivation of Bohai Sea Gulf fresh water shrimp, application is wider. At present, on market, conventional substrate modifier has: gemmaThe probio such as bacillus, enterococcus faecalis; Enzyme preparation, adsorbent, synergist; Shitosan, fulvic acid, sodium humate, bentonite, raw stoneAsh and chlorinated product etc. These products to a certain extent all can regulating water quality, improve substrate, but respectively has deficiency, quick limeEffectively simple, but complex operation, and effect is unstable; Chlorinated product effect is better, but excitant is large, contaminated environment; Enzyme preparationNeed regularly to add with probiotics, cost is higher, and quality is unstable.
Therefore, in aquaculture in the urgent need to developing a kind of green, safe and effective substrate modifier, especially particleGranulate, not thorough to solve pond bottom sterilization, the problem that bottom material of pond for breeding worsens.
(3) summary of the invention
The object of the invention is to solve bottom material of pond for breeding sterilization not thorough, the problem that bottom material of pond for breeding worsens, in oneThe bacteria resistance function of drug composition, provide a kind of for the green of sediment sterilization, safely, the Chinese herbal medicine particle that has no side effectPreparation and preparation method thereof.
The present invention is achieved through the following technical solutions:
A kind of sterilization comprises the steps: by the preparation method of herbal composite
1. filter out the Chinese medicine that can suppress Aeromonas hydrophila and vibrio parahaemolytious, rheum officinale, golden cypress, the coptis, cloves,Peppermint, lemongrass, thyme.
2. by the multiple medicinal material filtering out, by orthogonal test, carry out the antibacterial of Aeromonas hydrophila and vibrio parahaemolytiousTest, carries out significance analysis, obtains suitable prescription proportioning.
3. choose rheum officinale, golden cypress, the coptis according to parts by weight weighing, after mixing, put into Chinese medicine extracting tank, firstInferior extraction: add medicinal material weight 10-15 water doubly, extract 100 DEG C of temperature, wait while being warmed up to 100 DEG C, start timing, constant temperature timeFor 1-2h, filter will be inputted in original fluid container after medical filtration. Extract for the second time: add medicinal material weight 6-10 water doubly, extract temperatureDegree is 100 DEG C, reaches 100 DEG C and starts timing, and constant temperature time is 1-2h, by filter, medical filtration is inputted in original fluid container.In original fluid container, concentrate, being concentrated into medicinal material weight and concentrate weight is to stop for 1: 1 o'clock, obtains concentrated medicament.
4. choose cloves, peppermint, lemongrass, thyme according to parts by weight weighing, respectively or after mixing, put into Chinese medicineIn extractor, add medicinal material weight 8-12 water doubly, wait while being warmed up to 100 DEG C, start timing, boil 4-5 hour, condensation is collected and is wavedHair oil, after the volatile oil of collecting is mixed with appropriate alcohol, puts into freezer stand-by.
5. take auxiliary material according to following ratio of weight and number
By glucose, medical stone, dextrin, cornstarch adds three-dimensional hybrid fitness machine, mixes 1-2 hour, as carrier.Mixed carrier is placed on disk pelletizing machine. The concentrate that rheum officinale, golden cypress, the coptis are extracted, according to parts by weightThe ratio of 2%-8% is sprayed on outward on carrier, uses disk pelletizing machine carrier to be made to the Chinese herbal medicine particle of 2mm-8mm.
6. the Chinese herbal medicine particle making is put into 60 DEG C of-70 DEG C of baking ovens and dried 4-8 hour (moisture is less than 8%).Use spherical mixer, the volatile oil that cloves, peppermint, lemongrass, thyme are extracted is according to outside the ratio of parts by weight 2%-6%Be sprayed on particle surface, pack into fast in the aluminium foil bag of 500 grams/bag, press bag sealing, to obtain final product.
Detailed description of the invention
By more specific description the present invention, technical matters of the present invention and using method are easier to by the following exampleUnderstand.
Embodiment 1
1. filter out the Chinese medicine that can suppress Aeromonas hydrophila and vibrio parahaemolytious, rheum officinale, golden cypress, the coptis, cloves,Peppermint, lemongrass, thyme.
2. by the multiple medicinal material filtering out, by orthogonal test, carry out the antibacterial of Aeromonas hydrophila and vibrio parahaemolytiousTest, carries out significance analysis, obtains suitable prescription proportioning.
3. take by weight:
Rheum officinale 300g
Golden cypress 200g
Coptis 100g
After medicinal material is mixed, put into Chinese medicine extracting tank, extract for the first time: add 6L water, extract 100 DEG C of temperature, wait literTemperature during to 100 DEG C, starts timing, and constant temperature time is 1.5h, and 4 layers of filtered through gauze are poured in fluid reservoir. Extract for the second time: add 4.8LWater, extract temperature be 100 DEG C, reach 100 DEG C start timing, constant temperature time is 1.5h, 4 layers of filtered through gauze are poured fluid reservoir intoIn. In fluid reservoir, heat and concentrate, in the time that concentrate is 600ml, stop.
4. take according to weight,
After mixing, put into Chinese medicine extracting tank, add 4L water, wait while being warmed up to 100 DEG C, start timing, boil 4-5Hour, volatile oil is collected in condensation, after the volatile oil 1L collecting is mixed with the alcohol of 1L, puts into freezer stand-by.
5. take according to following weight:
Glucose, medical stone, dextrin, cornstarch are added to three-dimensional hybrid fitness machine, mix 1 hour, as carrier. WillMixed carrier is placed on disk pelletizing machine. The concentrate 600ml that rheum officinale, golden cypress, the coptis are extracted is sprayed on outward on carrier,Use disk pelletizing machine carrier to be made to the Chinese herbal medicine particle of 4mm.
6. the Chinese herbal medicine particle making is put into 60 DEG C of baking ovens and dried 7 hours (moisture is 6.38%). Use ballShape mixer, the volatile oil 1.2L that cloves, peppermint, lemongrass, thyme are extracted is sprayed on the particle surface of having dried outward, fast quick-mountingEnter in the aluminium foil bag of 500 grams/bag, press bag sealing, obtain this product.
Embodiment 2: the fungistatic effect of Chinese herbal medicine particle of the present invention is investigated
1 test objective: detect the fungistatic effect of Chinese herbal medicine particle of the present invention for Aeromonas hydrophila and vibrio parahaemolytious.
2, instrument and medicine
2.1 test apparatuses: clean bench, autoclave, growth cabinet, electronic analytical balance
2.2 test strains: Aeromonas hydrophila, vibrio parahaemolytious. Common purchased from China Microbial Culture Preservation CommissionMicroorganism center.
2.3 reagent: sodium chloride (analyzing pure), beef extract (biochemical level), peptone (biochemical level), Chinese herbal medicine particle of the present inventionProvided by Beijing Centre Biology Co., Ltd..
3 test methods:
The preparation of 3.1 bacteria suspensions: Aeromonas hydrophila is inoculated on beef-protein medium to vibrio parahaemolytiousBacterial classification is inoculated in 3% high salt beef-protein medium, cultivates 16-24 hour in 30 DEG C. Being diluted to concentration is 105-106The bacteria suspension (aseptic 8.5 ‰ physiological saline gradient dilutions) of cfu/ml.
Beef-protein medium formula
3.2 test operation programs:
Be divided into 8 groups by 2 kinds for examination bacterium liquid, every group of 2 repetitions, are respectively blank group, positive controls (10% poly-dimensionKetone iodine solution 0.5ppm), 0.5ppm groups of grains of the present invention and 1ppm groups of grains of the present invention (specifically grouping situation is in table 1), addAfter drug effect 1h, all shake up, sampling, carries out count plate respectively, calculates bacteriostasis rate.
The grouping of table 1 bacteriostatic test
| Numbering | Aeromonas hydrophila bacterium liquid Chinese traditional medicine adds concentration | Numbering | Vibrio parahaemolytious bacterium liquid Chinese traditional medicine adds concentration |
| 1 group | 0 (blank group) | 5 groups | 0 (blank group) |
| 2 groups | 10% PVP-I 0.5ppm | 6 groups | 10% PVP-I 0.5ppm |
| 3 groups | Particle 0.5ppm of the present invention | 7 groups | Particle 0.5ppm of the present invention 3 --> |
| 4 groups | Particle 1ppm of the present invention | 8 groups | Particle 1ppm of the present invention |
4, experimental result and discussion:
Table 2 bacteriostatic test result
5, conclusion: after particle of the present invention uses 1 hour for Aeromonas hydrophila, 0.5ppm dosage group bacteriostasis rate is57.83%, 1ppm dosage group bacteriostasis rate is 86.75%, all higher than the bacteriostasis rate of 10% Betagen Solution 0.5ppm dosage group(40.96%). After particle of the present invention uses 1 hour for vibrio parahaemolytious, 0.5ppm dosage group bacteriostasis rate is 45.71%,1ppm dosage group bacteriostasis rate is 77.14%, all higher than 10% PVP-I bacteriostasis rate (34.29%). Therefore, for freshwater aquicultureRecommendation dosage be 200～300 grams/acre of rice, the RD of sea-farming is 300～500 grams/acre of rice.
Should be understood that, those of ordinary skill in the art can improve or change according to this explanation, and these improvementAll should belong to the protection domain of claims of the present invention with conversion.
Claims (10)
1. there is a composition for disinfective action, it is characterized in that by rheum officinale, golden cypress, the coptis, cloves, peppermint, lemongrass, thymeMake, their ratio of weight and number is:
2. composition according to claim 1, is characterized in that active component is, rheum officinale water extract, golden cypress water extraction are gotThing, coptis root aqueous extract, volatile clove oil, Herba Menthae Haplocalycis volatile oil, lemongrass volatile oil, thyme volatile oil.
3. there is a preparation for disinfective action, it is characterized in that adopting conventional method by the composition described in claim 1 or 2Make common formulation.
4. preparation as claimed in claim 3, is characterized in that, adds cornstarch, glucose, wheat feed grade meal stone, dextrinGranulation agent.
5. preparation as claimed in claim 4, is characterized in that, auxiliary material ratio of weight and number is
6. the preparation method of composition as claimed in claim 1 or 2, is characterized in that comprising the following step:
(1) rheum officinale, golden cypress, the coptis are respectively or mix and carry out water extraction and get;
(2) volatile oil is extracted in cloves, peppermint, lemongrass, thyme difference or mixing.
7. the preparation method of preparation as claimed in claim 3, is characterized in that, comprises the following step:
(1) rheum officinale, golden cypress, coptis difference or mixing water extract
(2) cloves, peppermint, lemongrass, thyme difference or extraction volatile oil
(3) extract in step (1) and (2) and glucose, medical stone, dextrin, cornstarch are mixed and made into granule.
8. preparation method as claimed in claim 7, is characterized in that comprising the following step:
(1) choose rheum officinale, golden cypress, the coptis according to ratio of weight and number weighing, after mixing, put into Chinese medicine extracting tank, for the first timeExtract: add the water of 8-12 times of quality, extract 100 DEG C of temperature, constant temperature time is 1-2h, and filter will be inputted stoste after medical filtrationIn tank; Extract for the second time: add the water of 6-10 times of quality, extracting temperature is 100 DEG C, reach 100 DEG C and start timing, constant temperature time is1-2h, inputs medical filtration in fluid reservoir by filter; In fluid reservoir, concentrate, be concentrated into medicinal material weight and concentratedLiquid weight is to stop for 1: 1 o'clock;
(2) choose cloves, peppermint, lemongrass, thyme according to ratio of weight and number weighing, after mixing, put into Chinese medicine extracting tankIn, add 8-12 water doubly, wait and be warmed up to 100 DEG C, start timing, boil 4-5 hour, volatile oil is collected in condensation, by what collectVolatile oil is put into freezer stand-by after mixing with appropriate alcohol;
(3) glucose, medical stone, dextrin, cornstarch are added to three-dimensional hybrid fitness machine, mix 1-2 hour, as carrier;Mixed carrier is put on disk pelletizing machine; The concentrate that rheum officinale, golden cypress, the coptis are extracted, according to 5%-15%'sRatio of weight and number is sprayed on carrier, uses disk pelletizing machine that carrier is made to the spheroidal Chinese medicinal granule that diameter is 2mm-8mm;
(4) Chinese medicinal granule making is put into 50 DEG C of-70 DEG C of baking ovens and dried 6-8 hour, moisture is less than 8%; Drop into ballShape mixer, the volatile oil that cloves, peppermint, lemongrass, thyme are extracted is sprayed on particle table outward according to the part by weight of 2%-8%Face, packs in the aluminium foil bag of 500 grams/bag, presses bag sealing, to obtain final product.
9. the composition of claim 1 or 2 is in the application of preparing in aquatic products substrate bacteriostatic agent.
10. the preparation described in claim 3 or 4 or 5 is in the application of preparing in aquatic products substrate bacteriostatic agent.
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| CN105424927B (en) * | 2015-11-09 | 2017-05-10 | 山东省海洋生物研究院 | Method for detecting vibrio parahaemolyticus |
| CN105285523A (en) * | 2015-11-27 | 2016-02-03 | 青岛海之源智能技术有限公司 | Feed additive for preventing haemorrhagic septicaemia of ictalurus punctatus and preparation method of feed additive |
| CN105613614A (en) * | 2015-12-17 | 2016-06-01 | 集美大学 | Phyteral micro-ecological conditioning agent for reducing pathogenic bacteria in aquaculture and preparation method of phyteral micro-ecological conditioning agent |
| CN105660742A (en) * | 2016-02-26 | 2016-06-15 | 杨胜楠 | Traditional Chinese medicine disinfectant for physical examination |
| CN106577893A (en) * | 2016-11-29 | 2017-04-26 | 青岛海之星生物科技有限公司 | Shrimp and crap breeding pond vibrio inhibitor and preparation method thereof |
| CN106912507A (en) * | 2017-03-14 | 2017-07-04 | 滁州嘉实农业科技有限公司 | A kind of champignon and preparation method for black pig pigsty |
| CN109292833A (en) * | 2017-11-10 | 2019-02-01 | 天津地天科技发展有限公司 | A kind of green agriculture antitoxin improver of water quality of book on Chinese herbal medicine used for aquiculture |
| CN112616862B (en) * | 2021-01-07 | 2021-08-03 | 清远海贝生物技术有限公司 | Compound plant extract for inhibiting vibrio parahaemolyticus in water environment and preparation method and application thereof |
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| CN102178862A (en) * | 2011-04-27 | 2011-09-14 | 北京生泰尔生物科技有限公司 | Preparation and quality detection methods of Xianglian disinfectant |
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| CN102178862A (en) * | 2011-04-27 | 2011-09-14 | 北京生泰尔生物科技有限公司 | Preparation and quality detection methods of Xianglian disinfectant |
Non-Patent Citations (1)
| Title |
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| 抗弧菌中草药的筛选及其活性化合物的分离纯化;李爱娟;《中国优秀硕士学位论文全文数据库（电子期刊）》;20090215(第2期);对比文件2摘要，第3.4.2节以及第5.2.6节 * |
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