CN103814955A - Oxybasis glauca extract product and application of oxybasis glauca in trapping of prodenia litura - Google Patents

Oxybasis glauca extract product and application of oxybasis glauca in trapping of prodenia litura Download PDF

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Publication number
CN103814955A
CN103814955A CN201410069571.0A CN201410069571A CN103814955A CN 103814955 A CN103814955 A CN 103814955A CN 201410069571 A CN201410069571 A CN 201410069571A CN 103814955 A CN103814955 A CN 103814955A
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CN
China
Prior art keywords
oxybasis
chenopodium glaucum
glauca
glaucum linn
prodenia litura
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CN201410069571.0A
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Chinese (zh)
Inventor
叶敏
陈建民
陈谐晷
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YUNNAN TAIJIA BIOTECHNOLOGY Co Ltd
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YUNNAN TAIJIA BIOTECHNOLOGY Co Ltd
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Priority to CN201410069571.0A priority Critical patent/CN103814955A/en
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Abstract

The invention relates to an oxybasis glauca extract product and an application of oxybasis glauca in trapping of prodenia litura, which belongs to the technical field of the biological prevention and treatment of insect pests. The content of the invention comprises the following steps: crushing the fresh oxybasis glauca leaves and immersing for 1-96 hours by absolute ethyl alcohol with ratio of 1:2-1:8 times (w/w), extracting for 1-30 minutes by auxiliary of supersonic wave, filtering an ethanol leaching liquor, and performing vacuum concentration at 25-50 DEG C to obtain an oxybasis glauca ethanol extract product, dissolving the oxybasis glauca ethanol extract product by absolute ethyl alcohol to prepare an oxybasis glauca extract product solution, and injecting the oxybasis glauca extract product solution into a silicone rubber carrier trapping the prodenia litura. The oxybasis glauca extract product has strong trapping effect, has the advantages of high activity and long persistent period, and has good trapping effect to prodenia litura. The invention provides a novel biological prevention and treatment method for prodenia litura.

Description

A kind of chenopodium glaucum linn extract and lure concentrated application prodenia litura
Technical field
The present invention relates to a Plant Extracts in the application luring in collection prodenia litura, be specifically related to a kind of chenopodium glaucum linn extract in the application luring in collection prodenia litura, belong to biological control of insect pests technical field.
Background technology
Chenopodium glaucum linn (Chenopodium glaucum) is Chenopodiaceae annual herb plant, is distributed widely in global Temperate Region in China, is born in by the limit, farmland, water channel ditch of height above sea level 540-1400m, wasteland, Plain, mountain valley etc.
About the application of chenopodium glaucum linn, south China is among the people to be had and sets it as the edible custom of edible wild herbs.Chenopodium glaucum linn can also be used as medicine, and has effect of clearing heat and promoting diuresis.Chenopodium glaucum linn belongs to weeds in field, and the research of its application in agricultural pest control is less, and so far there are no about chenopodium glaucum linn extract for luring the relevant report of collection prodenia litura.
Prodenia litura (Spodoptera litura) is a kind of universal Important Agricultural insect of Lepidoptera Noctuidae.China is except Qinghai, Xinjiang are not bright, and each province (autonomous region) has generation.The blade of nearly 300 Plants such as larval feeding sweet potato, cotton, taro, lotus, sesbania, soybean, tobacco, twill and Cruciferae and solanaceous vegetable etc., the serious harm crops yield and quality that particularly vegetables produce.About the control main dependence chemical control at present of prodenia litura, for the insecticide of prodenia litura control, food security and ecotope are all had to very important negative effect.
Insect baiting technique is the green prevention and control technology advocated of country in recent years, and its principle is the insect that lures collection field by discharging special volatile chemical, concentrates after it is assembled and kills, and reaches the object of pest control.For luring the substance classes of collection prodenia litura very limited, research and develop the novel inducing agent of prodenia litura at present, to the biological control of prodenia litura, significant.
The inventor's the experimental results shows, chenopodium glaucum linn ethanol extract has good attractive effect to prodenia litura.By literature search, have no the open source literature report identical with the present invention.
Summary of the invention
The present invention has overcome the deficiency to chenopodium glaucum linn purposes understanding and practice, and the application of chenopodium glaucum linn control prodenia litura is provided.
The present invention is achieved in that
A kind of chenopodium glaucum linn extract, the present invention is characterised in that: this chenopodium glaucum linn extract is the ethanol extract of chenopodium glaucum linn leaf, concrete grammar is, fresh chenopodium glaucum linn blade is pulverized, be 1: 2~1: 8 times according to weight ratio and add soaked in absolute ethyl alcohol 1~96 hour, then ultrasonic assisted extraction 1~30 minute, after filtering by alcohol extract in 25~50 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
The preferred way of ethanol extract of this chenopodium glaucum linn leaf is, fresh chenopodium glaucum linn blade is pulverized, be 1: 4 times according to weight ratio and add soaked in absolute ethyl alcohol 48 hours, then ultrasonic assisted extraction 10 minutes, after filtering by alcohol extract in 40 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
Chenopodium glaucum linn extract lures a concentrated application prodenia litura, and the present invention is characterised in that, using the ethanol extract of chenopodium glaucum linn leaf with absolute ethyl alcohol as dissolution with solvents, make content and be 1~40% chenopodium glaucum linn leaf ethanol extract solution, for luring collection prodenia litura.
Beneficial effect of the present invention is:
1, the utilization of chenopodium glaucum linn has been had to brand-new understanding, for prodenia litura control provides a kind of novel inducing agent.
2, chenopodium glaucum linn ethanol extract has strong attractive effect to prodenia litura, active high, the lasting period is long, and prodenia litura is had to the good collection effect that lures.
Below in conjunction with specific embodiment, the present invention is further explained.
Embodiment
A kind of chenopodium glaucum linn extract, the present invention is characterised in that: this chenopodium glaucum linn extract is the ethanol extract of chenopodium glaucum linn leaf, concrete grammar is, fresh chenopodium glaucum linn blade is pulverized, be 1: 2~1: 8 times according to weight ratio and add soaked in absolute ethyl alcohol 1~96 hour, then ultrasonic assisted extraction 1~30 minute, after filtering by alcohol extract in 25~50 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
The preferred way of ethanol extract of this chenopodium glaucum linn leaf is, fresh chenopodium glaucum linn blade is pulverized, be 1: 4 times according to weight ratio and add soaked in absolute ethyl alcohol 48 hours, then ultrasonic assisted extraction 10 minutes, after filtering by alcohol extract in 40 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
Parameter in the preparation method of chenopodium glaucum linn extract of the present invention is not the unique point described in embodiment, but the chenopodium glaucum linn extract obtaining according to method described in the technical program, all should be in the protection domain of patent of the present invention.
A kind of chenopodium glaucum linn extract lures concentrated application prodenia litura, use absolute ethyl alcohol as dissolution with solvents the ethanol extract of chenopodium glaucum linn leaf, make content and be 1~40% chenopodium glaucum linn leaf ethanol extract solution, a certain amount of extract solution is injected to commercially available special silicon rubber carrier, and the silicon rubber carrier that contains extract solution is placed in to the trapper that hangs field, for luring collection prodenia litura.Silicon rubber carrier is height 1.5cm, and diameter 1cm's is cylindrical, and silicon rubber carrier has the characteristic of adsorption extract solution.
Embodiment 1
Be 1%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for prodenia litura.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
1% chenopodium glaucum linn ethanol extract solution prodenia litura lure collection effect
Figure BDA0000470795340000021
Embodiment 2
Be 5%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for prodenia litura.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
5% chenopodium glaucum linn ethanol extract solution prodenia litura lure collection effect
Figure BDA0000470795340000031
Embodiment 3
Be 10%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for prodenia litura.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
10% chenopodium glaucum linn ethanol extract solution prodenia litura lure collection effect
Figure BDA0000470795340000032
Embodiment 4
Be 20%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for prodenia litura.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
20% chenopodium glaucum linn ethanol extract solution prodenia litura lure collection effect
Embodiment 5
Be 30%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for prodenia litura.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
30% chenopodium glaucum linn ethanol extract solution prodenia litura lure collection effect
Figure BDA0000470795340000041
Embodiment 6
Be 40%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for prodenia litura.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
40% chenopodium glaucum linn ethanol extract solution prodenia litura lure collection effect

Claims (3)

1. a chenopodium glaucum linn extract, it is characterized in that: this chenopodium glaucum linn extract is the ethanol extract of chenopodium glaucum linn leaf, concrete grammar is, fresh chenopodium glaucum linn blade is pulverized, be 1: 2~1: 8 times according to weight ratio and add soaked in absolute ethyl alcohol 1~96 hour, then ultrasonic assisted extraction 1~30 minute, after filtering by alcohol extract in 25~50 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
2. a kind of chenopodium glaucum linn extract according to claim 1, it is characterized in that: the preferred way of ethanol extract of this chenopodium glaucum linn leaf is, fresh chenopodium glaucum linn blade is pulverized, be 1: 4 times according to weight ratio and add soaked in absolute ethyl alcohol 48 hours, then ultrasonic assisted extraction 10 minutes, after filtering by alcohol extract in 40 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
3. a chenopodium glaucum linn extract according to claim 1 and 2 lures concentrated application prodenia litura, it is characterized in that, using the ethanol extract of chenopodium glaucum linn leaf with absolute ethyl alcohol as dissolution with solvents, make content and be 1~40% chenopodium glaucum linn leaf ethanol extract solution, for luring collection prodenia litura.
CN201410069571.0A 2014-02-27 2014-02-27 Oxybasis glauca extract product and application of oxybasis glauca in trapping of prodenia litura Pending CN103814955A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5058005A (en) * 1973-09-27 1975-05-20
CN101940208A (en) * 2009-07-09 2011-01-12 慈溪市森林病虫防治检疫站 Sex pheromone composite for luring prodenia litura and luring core thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5058005A (en) * 1973-09-27 1975-05-20
CN101940208A (en) * 2009-07-09 2011-01-12 慈溪市森林病虫防治检疫站 Sex pheromone composite for luring prodenia litura and luring core thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
冯正娣 等: "棉田斜纹夜蛾生物学特性调查", 《江苏农业科学》 *
郭金春 等: "31种植物提取物除草作用研究初报", 《西北农林科技大学学报(自然科学版)》 *

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Application publication date: 20140528