CN103783090A - Oxybasis glauca extract and application thereof in trapping spodoptera exigua - Google Patents
Oxybasis glauca extract and application thereof in trapping spodoptera exigua Download PDFInfo
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- CN103783090A CN103783090A CN201410068938.7A CN201410068938A CN103783090A CN 103783090 A CN103783090 A CN 103783090A CN 201410068938 A CN201410068938 A CN 201410068938A CN 103783090 A CN103783090 A CN 103783090A
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- chenopodium glaucum
- glaucum linn
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Abstract
The invention relates to an oxybasis glauca extract and applicationof in trapping spodoptera exigua, belonging to the technical field of pest biological control. Fresh oxybasis glauca leaves are ground, soaked in absolute ethyl alcohol with the weight ratio of 1:2-1:8 for 1-96h, subjected to ultrasonic-assisted extraction for 1-30h and filtered, and the ethyl alcohol extract liquid is subjected to vacuum concentration at the temperature of 25-50 DEG C so as to obtain the oxybasis glauca extract which is dissolved by the ethyl alcohol extract liquid and prepared into an oxybasis glauca extract solution with the weight ratio of 1-40%, and the oxybasis glauca extract solution is injected into a silicone rubber carrier for trapping the spodoptera exigua. The oxybasis glauca extract has a strong trapping effect on oxybasis glauca, and is high in activity and long in lasting period. The invention provides a novel biological control method for controlling oxybasis glauca.
Description
Technical field
The present invention relates to a Plant Extracts in the application luring in collection beet armyworm, be specifically related to a kind of chenopodium glaucum linn extract in the application luring in collection beet armyworm, belong to biological control of insect pests technical field.
Background technology
Chenopodium glaucum linn (Chenopodium glaucum) is Chenopodiaceae annual herb plant, is distributed widely in global Temperate Region in China, is born in by the limit, farmland, water channel ditch of height above sea level 540-1400m, wasteland, Plain, mountain valley etc.
About the application of chenopodium glaucum linn, south China is among the people to be had and sets it as the edible custom of edible wild herbs.Chenopodium glaucum linn can also be used as medicine, and has effect of clearing heat and promoting diuresis.Chenopodium glaucum linn belongs to weeds in field, and the research of its application in agricultural pest control is less, and so far there are no about chenopodium glaucum linn extract for luring the relevant report of collection beet armyworm.
Beet armyworm (Spodoptera exigua) is a kind of worldwide intermittent large polyphagous pest-insect take danger to vegetables as master occurring that distributes of Lepidoptera Noctuidae.The vegetables such as shallot, wild cabbage, Chinese cabbage, celery, cauliflower, carrot, asparagus, water spinach, three-coloured amaranth, capsicum, cowpea, cauliflower, eggplant, cabbage mustard, tomato, cabbage heart, a variety of Chinese cabbage, broccoli, spinach, radish are had to harm, most of China is economized (autonomous region) all generation, the yield and quality that serious harm vegetables produce.About the control main dependence chemical control at present of beet armyworm, for the insecticide of beet armyworm control, food security and ecotope are all had to very important negative effect.
Insect baiting technique is the green prevention and control technology advocated of country in recent years, and its principle is the insect that lures collection field by discharging special volatile chemical, concentrates after it is assembled and kills, and reaches the object of pest control.For luring the substance classes of collection beet armyworm very limited, research and develop the novel inducing agent of beet armyworm at present, to the biological control of beet armyworm, significant.
The inventor's the experimental results shows, chenopodium glaucum linn ethanol extract has good attractive effect to beet armyworm.By literature search, have no the open source literature report identical with the present invention.
Summary of the invention
The present invention has overcome the deficiency to chenopodium glaucum linn purposes understanding and practice, and the application of chenopodium glaucum linn control beet armyworm is provided.
The present invention is achieved in that
A kind of chenopodium glaucum linn extract, the present invention is characterised in that: this chenopodium glaucum linn extract is the ethanol extract of chenopodium glaucum linn leaf, concrete grammar is, fresh chenopodium glaucum linn blade is pulverized, be 1: 2～1: 8 times according to weight ratio and add soaked in absolute ethyl alcohol 1～96 hour, then ultrasonic assisted extraction 1～30 minute, after filtering by alcohol extract in 25～50 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
The preferred way of ethanol extract of this chenopodium glaucum linn leaf is, fresh chenopodium glaucum linn blade is pulverized, be 1: 4 times according to weight ratio and add soaked in absolute ethyl alcohol 48 hours, then ultrasonic assisted extraction 10 minutes, after filtering by alcohol extract in 40 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
Chenopodium glaucum linn extract lures a concentrated application beet armyworm, and the present invention is characterised in that, using the ethanol extract of chenopodium glaucum linn leaf with absolute ethyl alcohol as dissolution with solvents, make content and be 1～40% chenopodium glaucum linn leaf ethanol extract solution, for luring collection beet armyworm.
Beneficial effect of the present invention is:
1, the utilization of chenopodium glaucum linn has been had to brand-new understanding, for beet armyworm control provides a kind of novel inducing agent.
2, chenopodium glaucum linn ethanol extract has strong attractive effect to beet armyworm, active high, the lasting period is long, and beet armyworm is had to the good collection effect that lures.
Below in conjunction with specific embodiment, the present invention is further explained.
Embodiment
A kind of chenopodium glaucum linn extract, the present invention is characterised in that: this chenopodium glaucum linn extract is the ethanol extract of chenopodium glaucum linn leaf, concrete grammar is, fresh chenopodium glaucum linn blade is pulverized, be 1: 2～1: 8 times according to weight ratio and add soaked in absolute ethyl alcohol 1～96 hour, then ultrasonic assisted extraction 1～30 minute, after filtering by alcohol extract in 25～50 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
The preferred way of ethanol extract of this chenopodium glaucum linn leaf is, fresh chenopodium glaucum linn blade is pulverized, be 1: 4 times according to weight ratio and add soaked in absolute ethyl alcohol 48 hours, then ultrasonic assisted extraction 10 minutes, after filtering by alcohol extract in 40 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
Parameter in the preparation method of chenopodium glaucum linn extract of the present invention is not the unique point described in embodiment, but the chenopodium glaucum linn extract obtaining according to method described in the technical program, all should be in the protection domain of patent of the present invention.
A kind of chenopodium glaucum linn extract lures concentrated application beet armyworm, use absolute ethyl alcohol as dissolution with solvents the ethanol extract of chenopodium glaucum linn leaf, make content and be 1～40% chenopodium glaucum linn leaf ethanol extract solution, a certain amount of extract solution is injected to commercially available special silicon rubber carrier, and the silicon rubber carrier that contains extract solution is placed in to the trapper that hangs field, for luring collection beet armyworm.Silicon rubber carrier is height 1.5cm, and diameter 1cm's is cylindrical, and silicon rubber carrier has the characteristic of adsorption extract solution.
Embodiment 1
Be 1%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for beet armyworm.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
1% chenopodium glaucum linn ethanol extract solution beet armyworm lure collection effect
Embodiment 2
Be 5%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for beet armyworm.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
5% chenopodium glaucum linn ethanol extract solution beet armyworm lure collection effect
Embodiment 3
Be 10%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for beet armyworm.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
10% chenopodium glaucum linn ethanol extract solution beet armyworm lure collection effect
Embodiment 4
Be 20%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for beet armyworm.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
20% chenopodium glaucum linn ethanol extract solution beet armyworm lure collection effect
Embodiment 5
Be 30%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for beet armyworm.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
30% chenopodium glaucum linn ethanol extract solution beet armyworm lure collection effect
Embodiment 6
Be 40%(w/w by the ethanol extract of chenopodium glaucum linn with making content after anhydrous alcohol solution) chenopodium glaucum linn ethanol extract solution, in the chenopodium glaucum linn ethanol extract solution Implanted Silicon rubber carrier of different volumes, lure collection for beet armyworm.Lure liquid collecting (sugar: vinegar: wine: water=3: 4: 1: 2) to lure collection thing as contrasting take sweet and sour.
40% chenopodium glaucum linn ethanol extract solution beet armyworm lure collection effect
Claims (3)
1. a chenopodium glaucum linn extract, it is characterized in that: this chenopodium glaucum linn extract is the ethanol extract of chenopodium glaucum linn leaf, concrete grammar is, fresh chenopodium glaucum linn blade is pulverized, be 1: 2～1: 8 times according to weight ratio and add soaked in absolute ethyl alcohol 1～96 hour, then ultrasonic assisted extraction 1～30 minute, after filtering by alcohol extract in 25～50 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
2. a kind of chenopodium glaucum linn extract according to claim 1, it is characterized in that: the preferred way of ethanol extract of this chenopodium glaucum linn leaf is, fresh chenopodium glaucum linn blade is pulverized, be 1: 4 times according to weight ratio and add soaked in absolute ethyl alcohol 48 hours, then ultrasonic assisted extraction 10 minutes, after filtering by alcohol extract in 40 ℃ of reduced pressure concentrations, obtain the ethanol extract of chenopodium glaucum linn.
3. a chenopodium glaucum linn extract according to claim 1 and 2 lures concentrated application beet armyworm, it is characterized in that, using the ethanol extract of chenopodium glaucum linn leaf with absolute ethyl alcohol as dissolution with solvents, make content and be 1～40% chenopodium glaucum linn leaf ethanol extract solution, for luring collection beet armyworm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410068938.7A CN103783090A (en) | 2014-02-27 | 2014-02-27 | Oxybasis glauca extract and application thereof in trapping spodoptera exigua |
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| CN201410068938.7A CN103783090A (en) | 2014-02-27 | 2014-02-27 | Oxybasis glauca extract and application thereof in trapping spodoptera exigua |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986663A (en) * | 2012-12-24 | 2013-03-27 | 福建省农业科学院植物保护研究所 | Beet armyworm dual-sexual attractant |
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2014
- 2014-02-27 CN CN201410068938.7A patent/CN103783090A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986663A (en) * | 2012-12-24 | 2013-03-27 | 福建省农业科学院植物保护研究所 | Beet armyworm dual-sexual attractant |
Non-Patent Citations (2)
| Title |
|---|
| M. BERDEGUE等: "Effects of plant chemical extracts and physical characteristics of Apium graveolens and Chenopodium murale on host choice by Spodoptera exigua larvae", 《ENTOMOLOGIA EXPERIMENTALIS ET APPHCATA》, no. 78, 31 December 1996 (1996-12-31), pages 253 - 262 * |
| 冯艳波等: "南疆12种植物抗氧化成分的测定及其清除自由基作用研究", 《中国酿造》, vol. 32, no. 11, 15 November 2013 (2013-11-15), pages 94 - 97 * |
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Application publication date: 20140514 |