CN102499267B - Preparation and using methods of Scarabaeus pest food attractant - Google Patents

Preparation and using methods of Scarabaeus pest food attractant Download PDF

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Publication number
CN102499267B
CN102499267B CN 201110308667 CN201110308667A CN102499267B CN 102499267 B CN102499267 B CN 102499267B CN 201110308667 CN201110308667 CN 201110308667 CN 201110308667 A CN201110308667 A CN 201110308667A CN 102499267 B CN102499267 B CN 102499267B
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China
Prior art keywords
lixiviate
lure
preparation
scarab beetle
scarabaeus
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CN 201110308667
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CN102499267A (en
Inventor
曲明静
李晓
鞠倩
赵志强
姜晓静
杨伟强
禹山林
杨珍
宋文武
江晨
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Shandong Peanut Research Institute
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Shandong Peanut Research Institute
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Abstract

The invention discloses preparation and using methods of a Scarabaeus pest food attractant. The preparation method of the food attractant comprises the following steps of, firstly, collecting fresh elm leaves, grinding the fresh elm leaves in a grinder, extracting the ground fresh elm leaves with an extraction agent under the room temperature with the ratio of the extraction agent and the ground raw materials (V/W) of 3:1, and then filtering the extracted elm leaves; secondly, concentrating the extract liquid prepared in the step one by a rotary evaporator; and thirdly, adding 4ml concentrated extract liquid prepared in step two on an absorbent cotton ball with the diameter of 2cm to prepare a lure. The using method comprises the following steps that, firstly, the lure is hung in a collection bag which is arranged below a scarab trap; secondly, the collection bag is placed in a field from 19:30 every night to midnight; thirdly, during hanging, the height of the collection bag arranged below the scarab trap from the ground is from 2m to 4m; and fourthly, the using time is based on the emergence activity time of Scarabaeus overwintering imagoes. Main components of the attractant are derived from plants, and the Scarabaeus pest food attractant is not contacted with crops when being used, so that the attractant has no poison or pollution and is effective to all main Scarabaeus pests.

Description

A kind of preparation and using method of scarab beetle class pest food lure
Technical field
The present invention relates to a kind of food lure preparation and using method, relate in particular to a kind of preparation and using method of scarab beetle class pest food lure.
Background technology
The cockchafer Class A is the common main soil insect that dwells during agriculture and forestry are produced, various crop such as its larva grub harm peanut, soybean, pachyrhizus, corn, especially three big chafers: Holotrichia parallela, anomala corpulenta and black greatly gill cockchafer, because it distributes wide, feeding habits are assorted, disguised high, adaptability is strong, control is the emphasis and the difficult point of plant protection work always, the economic loss that causes every year is huge, the tradition controlling mode adopts chemical pesticide, not only produces problems such as persistent pesticide, insect develop immunity to drugs, contaminated environment, and far not only like this to people and animals' harm.
The worm new technology of controlling of insect pheromone or attractant pest control is used more and more widely in recent years, compares with chemical pesticide, and this technology adopts pure biological mode, can not produce problems such as high residue, pesticide resistance, contaminated environment, and is safer to people and animals.The usability pheromones lures adult that a lot of application reports have been arranged, this technology can reach the purpose that the larva insect density takes place and reduces the monitoring adult, play the effect that soil insect is controlled on the ground, but gyplure has the selectivity and the specificity of height, can only prevent and treat at a certain insect, and gyplure can only be traped male worm or female worm, it is its limitation part, especially inadequate on control scarab beetle class pest, with the Holotrichia parallela is example, the repeatedly mating of female worm is so the gyplure trapping can only be played the effect that reduces the male worm mating rate.Because above-mentioned several scarab beetle class pests are lured the comparison sensitivity to food, so adopting the food lure to kill this class pest is one of best approach, but how to make and use scarab beetle class pest food lure to lure field scarab beetle class pest, its product slaughtered and technology are not appeared in the newspapers yet.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of preparation and using method of scarab beetle class pest food lure is provided.
For achieving the above object, the technology used in the present invention means are that the preparation method is as follows for this food lure:
, gather fresh leaf of elm tree, add in the cracker and pulverize, add the lixiviate of lixiviate reagent, institute adds lixiviate reagent and (v/w) that pulverize the back raw material than being 3:1, lixiviate under the room temperature, then filtration;
Two, step 1 is made leaching liquor, be concentrated into 1/4~1/16 of original volume with Rotary Evaporators;
Three, step 2 gained lixiviate concentrate 4ml is added to make on the rayon balls of diameter 2cm and lures core.
The using method of this food lure is as follows:
One, will lure core to be suspended in the collecting bag of scarab beetle trapper below;
Two, place the field with 19:30 every night to morning next day;
When three, hanging, scarab beetle trapper below collecting bag is 2m~4m apart from ground level;
Can begin when four, survive the winter the adult eclosion activity with cockchafer service time.
The invention has the beneficial effects as follows: food lure main component derives from plant, and consumption is few, does not directly contact with crop, and crop is not produced murder by poisoning; Environment and crop are not had influence, be particularly useful for the production of green organic food; All can lure female male worm, and lure the worm kind many, all effective to the main scarab beetle class pest of damage to crops; The insect of being traped can be used for feeding other purposes such as chicken, has improved economic benefit.
Embodiment
Embodiment 1
A kind of preparation and using method of scarab beetle class pest food lure, described food lure preparation method is as follows:
One, gather fresh leaf of elm tree, add in the cracker and pulverize, take by weighing 20g after the pulverizing, add lixiviate reagent carrene 60ml, lixiviate 24h under the room temperature then filters;
Two, step 1 is made leaching liquor, be concentrated into 1/4 of original volume with RE52CS-1 type Rotary Evaporators;
Three, step 2 gained lixiviate concentrate 4ml is added to make on the rayon balls of diameter 2cm and lures core.
The using method of this food lure is as follows:
One, will lure core to be suspended in the collecting bag of scarab beetle trapper below, used trapper is patent No. ZL 200920217763.6 disclosed cockchafer subtype trappers;
Two, place the peanut field with 19:30 every night to morning next day, 60m at interval between the trapper;
When three, hanging, scarab beetle trapper below collecting bag is 2m apart from ground level;
Bring into use when four, surviving the winter the adult eclosion activity with cockchafer.
Statistical data is an example when getting experiment July 17, knocking off morning in 2010 in the date, and quantity that each trapper is pounced on is 106 ± 22/platform, and male and female are than being 1:1.07, far above not using 6 ± 2/platform eating lure.
Embodiment 2
A kind of preparation and using method of scarab beetle class pest food lure, described food lure preparation method is as follows:
One, gathers fresh leaf of elm tree, add in the cracker and pulverize, take by weighing 20g after the pulverizing, add lixiviate reagent carrene 60ml, with the auxiliary lixiviate of KQ-300VDE type ultrasonic washing instrument, set 20 ℃ of ultrasonic temperature, frequency 28kHz, power 150W, concussion 25min filters then;
Two, step 1 is made leaching liquor, be concentrated into 1/16 of original volume with RE52CS-1 type Rotary Evaporators;
Three, step 2 gained lixiviate concentrate 4ml is added to make on the rayon balls of diameter 2cm and lures core.
The using method of this food lure is as follows:
One, will lure core to be suspended in the collecting bag of scarab beetle trapper below, used trapper is patent No. ZL 200920217763.6 disclosed cockchafer subtype trappers;
Two, place the peanut field with 19:30 every night to morning next day, 60m at interval between the trapper;
When three, hanging, scarab beetle trapper below collecting bag is 3m apart from ground level;
Bring into use when four, surviving the winter the adult eclosion activity with cockchafer.
Statistical data is an example when getting experiment July 17, knocking off morning in 2010 in the date, and quantity that each trapper is pounced on is 147 ± 33/platform, and male and female are than being 1:1.02, far above not using 6 ± 2/platform eating lure.
Embodiment 3
A kind of preparation and using method of scarab beetle class pest food lure, described food lure preparation method is as follows:
One, gathers fresh leaf of elm tree, add in the cracker and pulverize, take by weighing 20g after the pulverizing, add lixiviate reagent acetone 60ml, lixiviate under the room temperature with the auxiliary lixiviate of KQ-300VDE type ultrasonic washing instrument, is set 20 ℃ of ultrasonic temperature, frequency 28kHz, power 150W, concussion 25min filters then;
Two, step 1 is made leaching liquor, be concentrated into 1/8 of original volume with RE52CS-1 type Rotary Evaporators;
Three, step 2 gained lixiviate concentrate 4ml is added to make on the rayon balls of diameter 2cm and lures core.
The using method of this food lure is as follows:
One, will lure core to be suspended in the collecting bag of scarab beetle trapper below, used trapper is patent No. ZL 200920217763.6 disclosed cockchafer subtype trappers;
Two, place the peanut field with 19:30 every night to morning next day, 60m at interval between the trapper;
When three, hanging, scarab beetle trapper below collecting bag is 4m apart from ground level;
Bring into use when four, surviving the winter the adult eclosion activity with cockchafer.
Statistical data is an example when getting experiment July 17, knocking off morning in 2010 in the date, and quantity that each trapper is pounced on is 102 ± 28/platform, and male and female are than being 1:0.95, far above not using 6 ± 2/platform eating the lure blank.
Embodiment 4
A kind of preparation and using method of scarab beetle class pest food lure, described food lure preparation method is as follows:
One, gathers fresh leaf of elm tree, add in the cracker and pulverize, take by weighing 20g after the pulverizing, add lixiviate reagent n-hexane 60ml, lixiviate under the room temperature with the auxiliary lixiviate of KQ-300VDE type ultrasonic washing instrument, is set 20 ℃ of ultrasonic temperature, frequency 28kHz, power 150W, concussion 25min filters then;
Two, step 1 is made leaching liquor, be concentrated into 1/10 of original volume with RE52CS-1 type Rotary Evaporators;
Three, step 2 gained lixiviate concentrate 4ml is added to make on the rayon balls of diameter 2cm and lures core.
The using method of this food lure is as follows:
One, will lure core to be suspended in the collecting bag of scarab beetle trapper below, used trapper is patent No. ZL 200920217763.6 disclosed cockchafer subtype trappers;
Two, place the peanut field with 19:30 every night to morning next day, 60m at interval between the trapper;
When three, hanging, scarab beetle trapper below collecting bag is 2m apart from ground level;
Bring into use when four, surviving the winter the adult eclosion activity with cockchafer.
Statistical data is an example when getting experiment July 17, knocking off morning in 2010 in the date, and quantity that each trapper is pounced on is 133 ± 35/platform, and male and female are than being 1:1.05, far above not using 6 ± 2/platform eating lure.
As can be seen from the above embodiments, food lure provided by the present invention lures the worm Considerable effect, and the scarab beetle class pest of being lured comprises Holotrichia parallela, anomala corpulenta; And female male imago all had attracting action, and the scarab beetle adult female-male proportion that lures meets the property ratio of its natural population.In view of the omnivorousness of luring insect, lure provided by the present invention can also be in the soybean field, the melon patch, ground, and use in the crop field that other scarab beetle such as corn field is caused harm.
More than listed embodiment only for some embodiment of the present invention is described, those skilled in the art in the change of carrying out on the basis of the present invention that does not break away from flesh and blood of the present invention also in protection domain of the present invention.

Claims (5)

1. the preparation method of scarab beetle class pest food lure, it is characterized in that: the preparation method is as follows for this food lure:
One, gather fresh leaf of elm tree, add in the cracker and pulverize, add the lixiviate of lixiviate reagent, the volume that institute adds lixiviate reagent is 3:1 with the mass ratio of pulverizing the back raw material, lixiviate under the room temperature, then filtration;
Two, step 1 is made leaching liquor, concentrate with Rotary Evaporators;
Three, step 2 gained lixiviate concentrate 4ml is added to make on the rayon balls of diameter 2cm and lures core; Wherein
Described lixiviate reagent is a kind of in carrene, acetone, three kinds of organic solvents of n-hexane.
2. the preparation method of scarab beetle class pest food lure according to claim 1, it is characterized in that: lixiviate under the described room temperature, do not use the auxiliary equipment time to be 24h or the auxiliary lixiviate of use KQ-300VDE type ultrasonic washing instrument, concussion 25min when 20 ℃ of ultrasonic temperature, frequency 28kHz, power 150W.
3. the preparation method of scarab beetle class pest food lure according to claim 1, it is characterized in that: described Rotary Evaporators is selected the RE52CS-1 type for use, and leaching liquor is concentrated into 1/4~1/16 of original volume.
4. the using method of a scarab beetle class pest according to claim 1 food lure, it is characterized in that: the using method of this food lure is as follows:
One, will lure core to be suspended in the collecting bag of scarab beetle trapper below;
Two, place the field with 19:30 every night to morning next day;
When three, hanging, scarab beetle trapper below collecting bag is 2m~4m apart from ground level;
Can begin when four, survive the winter the adult eclosion activity with cockchafer service time.
5. the using method of scarab beetle class pest food lure according to claim 4, it is characterized in that: the spacing distance of described scarab beetle trapper is arranged on 60m.
CN 201110308667 2011-10-13 2011-10-13 Preparation and using methods of Scarabaeus pest food attractant Expired - Fee Related CN102499267B (en)

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Publication number Priority date Publication date Assignee Title
CN103371137B (en) * 2011-10-13 2014-12-17 山东省花生研究所 Use method of food attractant for scarab species
CN114431230A (en) * 2022-01-17 2022-05-06 江苏凯元科技有限公司 Plant source pesticide surfactant and preparation method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1903032A (en) * 2006-07-28 2007-01-31 河南农业大学 Broad spectrum attractant for tumblebug perfect insects
CN101190006A (en) * 2006-11-30 2008-06-04 河北省农林科学院植物保护研究所 Method for selecting and biological detecting high toxicity Bt new bacterial strain and gene of scarabaeus

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1903032A (en) * 2006-07-28 2007-01-31 河南农业大学 Broad spectrum attractant for tumblebug perfect insects
CN101190006A (en) * 2006-11-30 2008-06-04 河北省农林科学院植物保护研究所 Method for selecting and biological detecting high toxicity Bt new bacterial strain and gene of scarabaeus

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暗黑鳃金龟成虫对非寄主蓖麻和几种寄主叶片的选择和取食反应;李为争等;《河南农业大学学报》;20100831;第44卷(第4期);第438-442、447页 *
李为争等.暗黑鳃金龟成虫对非寄主蓖麻和几种寄主叶片的选择和取食反应.《河南农业大学学报》.2010,第44卷(第4期),第438-442、447页.
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Address after: 266100, Shandong Province, Qingdao City, Laixi Province town shop No. 23 Hing Road

Applicant after: Shandong Peanut Inst.

Address before: 266100, No. 64, Wangcheng Road, Laixi, Shandong, Qingdao

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