CN103789411A - 中国家猪与西方商品猪资源鉴定位点及其位点扩增引物 - Google Patents
中国家猪与西方商品猪资源鉴定位点及其位点扩增引物 Download PDFInfo
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- CN103789411A CN103789411A CN201310723082.8A CN201310723082A CN103789411A CN 103789411 A CN103789411 A CN 103789411A CN 201310723082 A CN201310723082 A CN 201310723082A CN 103789411 A CN103789411 A CN 103789411A
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- pig
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- 230000003321 amplification Effects 0.000 title claims abstract description 7
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 7
- 241000282898 Sus scrofa Species 0.000 title abstract description 40
- 239000002773 nucleotide Substances 0.000 claims abstract description 5
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 5
- 239000012634 fragment Substances 0.000 claims description 3
- 239000003550 marker Substances 0.000 abstract description 17
- 238000000034 method Methods 0.000 abstract description 9
- 241000282887 Suidae Species 0.000 abstract description 7
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000002068 genetic effect Effects 0.000 abstract description 3
- 238000001823 molecular biology technique Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000003147 molecular marker Substances 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 39
- 241000282894 Sus scrofa domesticus Species 0.000 description 33
- 238000006243 chemical reaction Methods 0.000 description 10
- 239000000203 mixture Substances 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 238000000546 chi-square test Methods 0.000 description 4
- 229910021642 ultra pure water Inorganic materials 0.000 description 4
- 239000012498 ultrapure water Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 108091092878 Microsatellite Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 108010007577 Exodeoxyribonuclease I Proteins 0.000 description 1
- 101150015860 MC1R gene Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 244000144980 herd Species 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
超纯水 | 460ul |
Mgcl2 | 234ul |
10×PCR buffer | 116ul |
Fast taq(5U/ul) | 24ul |
dNTP(10mM each) | 8ul |
扩增引物 | 8ul |
ExoⅠ(50U/ul) | 60ul |
SAP(1U/ul) | 130ul |
10×SAP buffer | 35ul |
超纯水 | 125ul |
37 ℃ | 40min |
96 ℃ | 10min |
SnaPshot multiplex yeady reaction mix | 100ul |
延伸引物 | 8ul |
5×sequencing buffer | 200ul |
超纯水 | 300ul |
SAP(1U/ul) | 120ul |
10×SAP buffer | 70ul |
超纯水 | 140ul |
37℃ | 40min |
75℃ | 20min |
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310723082.8A CN103789411B (zh) | 2013-12-25 | 2013-12-25 | 中国家猪与西方商品猪资源鉴定位点及其位点扩增引物 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310723082.8A CN103789411B (zh) | 2013-12-25 | 2013-12-25 | 中国家猪与西方商品猪资源鉴定位点及其位点扩增引物 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103789411A true CN103789411A (zh) | 2014-05-14 |
CN103789411B CN103789411B (zh) | 2015-12-30 |
Family
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Family Applications (1)
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CN201310723082.8A Expired - Fee Related CN103789411B (zh) | 2013-12-25 | 2013-12-25 | 中国家猪与西方商品猪资源鉴定位点及其位点扩增引物 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103789411B (zh) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105256035A (zh) * | 2015-10-26 | 2016-01-20 | 浙江省农业科学院 | 用于鉴别金华两头乌和杜洛克猪遗传背景的分子标记及其应用 |
CN105603089A (zh) * | 2016-02-03 | 2016-05-25 | 漳州傲农现代农业开发有限公司 | 一组用于鉴别猪种的snp标记及其应用 |
CN106244719A (zh) * | 2016-10-11 | 2016-12-21 | 贵州大学 | 一种鉴别香猪品种的结构变异sv141分子标记及其应用 |
CN117051115A (zh) * | 2022-09-20 | 2023-11-14 | 哈尔滨海关技术中心 | 用于进出境牛和猪种属和种系鉴定的引物组及应用 |
-
2013
- 2013-12-25 CN CN201310723082.8A patent/CN103789411B/zh not_active Expired - Fee Related
Non-Patent Citations (3)
Title |
---|
GUDALGO AM ET AL: "five mapping and single nucleotide polymorphism effects estimation on pig chromosomes 1,4,7,8,17 and x", 《GENET MOL BIOL》, vol. 36, no. 4, 8 November 2013 (2013-11-08), pages 511 - 519 * |
MATSUMOTO T ET AL: "porcine single nucleotide polymorphism (SNP) development and population structure of pigs assessed by validated SNPs", 《BIOCHEM GENET》, vol. 50, no. 56, 15 December 2011 (2011-12-15), pages 428 - 439 * |
RUSSO V ET AL: "use of single nucleotide polymorphisms to study variability in local and cosmopolitan pig breeds reared in italy", 《OPTIONS MEDITERRANEENNES》, vol. 76, 31 December 2007 (2007-12-31), pages 61 - 65 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105256035A (zh) * | 2015-10-26 | 2016-01-20 | 浙江省农业科学院 | 用于鉴别金华两头乌和杜洛克猪遗传背景的分子标记及其应用 |
CN105256035B (zh) * | 2015-10-26 | 2018-08-10 | 浙江省农业科学院 | 用于鉴别金华两头乌和杜洛克猪遗传背景的分子标记及其应用 |
CN105603089A (zh) * | 2016-02-03 | 2016-05-25 | 漳州傲农现代农业开发有限公司 | 一组用于鉴别猪种的snp标记及其应用 |
CN106244719A (zh) * | 2016-10-11 | 2016-12-21 | 贵州大学 | 一种鉴别香猪品种的结构变异sv141分子标记及其应用 |
CN117051115A (zh) * | 2022-09-20 | 2023-11-14 | 哈尔滨海关技术中心 | 用于进出境牛和猪种属和种系鉴定的引物组及应用 |
CN117051115B (zh) * | 2022-09-20 | 2024-04-30 | 哈尔滨海关技术中心 | 用于进出境牛和猪种属和种系鉴定的引物组及应用 |
Also Published As
Publication number | Publication date |
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CN103789411B (zh) | 2015-12-30 |
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C53 | Correction of patent of invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhang Yaping Inventor after: Xie Haibing Inventor after: Han Xuman Inventor after: Gao Yun Inventor before: Zhang Yaping Inventor before: Xie Haibing Inventor before: Han Xuman |
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COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: ZHANG YAPING XIE HAIBING HAN XUMAN TO: ZHANG YAPING XIE HAIBING HAN XUMAN GAO YUN |
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C14 | Grant of patent or utility model | ||
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CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151230 Termination date: 20171225 |