CN103749966A - Potato residue fermented feed and preparation method thereof - Google Patents
Potato residue fermented feed and preparation method thereof Download PDFInfo
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- CN103749966A CN103749966A CN201410039337.3A CN201410039337A CN103749966A CN 103749966 A CN103749966 A CN 103749966A CN 201410039337 A CN201410039337 A CN 201410039337A CN 103749966 A CN103749966 A CN 103749966A
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- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
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- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
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- Fodder In General (AREA)
Abstract
The invention discloses a potato residue fermented feed and a preparation method thereof, and relates to the field of animal feed prepared from plant materials. Taking potato residue 75-80%, dried jujube powder 10-15% and defatted rice bran 5-15% as raw and auxiliary materials, adding 0.75-1.5% of urea, 0.75-1.5% of ammonium sulfate, 0.5-0.7% of potassium dihydrogen phosphate and 0.4-0.6% of magnesium sulfate on the basis, mixing to obtain a fermentation substrate, preparing a combined zymogen bacterial solution by using bacterial solutions of bacillus subtilis, saccharomycete, aspergillus niger and lactobacillus plantarum, and performing aerobic fermentation and then anaerobic fermentation to obtain the product; the weight ratio of the fermentation substrate to the combined zymocyte bacterial liquid is 1: 1-1.2; the volume ratio of the four bacterial liquids is 1-3:1-3:1-3: 1-3. The invention can increase the true protein content of the fermentation substrate, reduce the crude fiber content, improve the palatability and the digestion utilization rate of the feed, comprehensively improve the nutritional value of the potato residue and have double benefits of economic and environmental benefits.
Description
Technical field
The technical field of animal that the present invention relates to make from vegetable material.
Background technology
China is potato planting the world's strongest nation.In China, potato is except directly eating, be mainly used to produce starch, 1 ton of farina of every production can produce the fresh slag of 7000kg left and right, and fresh potato slag water content is up to 90%, carry miscellaneous bacteria many, be difficult for storing, transportation, but contain again a small amount of protein and much starch, cellulose, pectin etc. in dry, can utilize composition, if not being used, not only can cause the wasting of resources, also can be to environment.If dry and be directly used as animal feed, cost is high and protein content is low, and crude fiber content is high, and palatability is poor.Therefore, develop safe and efficient effective potato residues earned value techniques, improve its nutritive value and security, there is economy and environment double benefit.
By biotechnology, particularly microbial fermentation technology is processed feedstuff, is improved nutritive value, is the safe and efficient method of low-quality feed resource exploitation.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of potato residues fermented feed and preparation method thereof, can increase the true protein content of fermentation substrate, reduce crude fibre amount, improve feed palatability and digestive utilization ratio, improve potato residues nutritive value comprehensively, there is economy and environment double benefit.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of potato residues fermented feed, with potato residue 75-80wt%, dry jujube powder 10-15wt% and defatted rice bran 5-15wt% are supplementary material, on this supplementary material basis, add reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, be mixed to get fermentation substrate, with bacillus subtilis, saccharomycopsis fibuligera, the bacterium liquid of aspergillus niger and Lactobacillus plantarum is mixed into combined fermentation bacterial classification bacterium liquid, first aerobic fermentation, anaerobic fermentation is prepared from again, water content≤the 14wt% of described potato residue, the weight ratio of fermentation substrate and combined fermentation bacterial classification bacterium liquid is 1:1-1.2, the bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is 1 × 10
6individual/ml, the ratio that combined fermentation bacterial classification bacterium liquid is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes.
Preferably, the ratio that combined fermentation bacterial classification bacterium liquid is 3:3:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes.
The preparation method of potato residues fermented feed, comprises the following steps:
(1) take potato residue 75-80wt%, dry jujube powder 10-15wt% and defatted rice bran 5-15wt% as supplementary material, on this supplementary material basis, add reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, mix, make fermentation substrate; Water content≤the 14wt% of described potato residue.
(2) above-mentioned fermentation substrate is mixed into combined fermentation bacterial classification bacterium liquid with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum, fermentation substrate evenly mixes by the weight ratio of 1:1-1.2 with combined fermentation bacterial classification bacterium liquid, 28-30 ℃ of aerobic fermentation 48-72 hour, then anaerobic fermentation 24-48 hour, dries and pulverizes; The bacterial concentration of described bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is 1 × 10
6individual/ml, the ratio that combined fermentation bacterial classification bacterium liquid is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes.
Preferably, the ratio that combined fermentation bacterial classification bacterium liquid is 3:3:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes.
Bacillus subtilis (Bacillus subtilis) 10075, aspergillus niger (Aspergillus niger) 40094, is purchased from Chinese industrial microorganism fungus kind preservation administrative center; Saccharomycopsis fibuligera (Endomycopsis fibuligera) 20154, Lactobacillus plantarum (Lactobacillus plantarum) 11016, be purchased from Chinese agriculture microorganism fungus kind preservation administrative center.
The preparation of bacillus subtilis used, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum bacterium liquid can adopt prior art, the invention provides following methods.
1, the preparation of Lactobacillus plantarum bacterium liquid
The Lactobacillus plantarum that 4 ℃ of inclined-planes are preserved is connected on MRS liquid tube culture medium and activates, at 37 ℃ with 220rmin
-1shaken cultivation 24h, cultivates seed as one-level; With 1% inoculum concentration, be connected in MRS triangular flask fluid nutrient medium again, at 37 ℃ with 220rmin
-1shaken cultivation 24h.MRS culture medium: peptone 10 g, beef extract 10 g, yeast extract 5 g, hydrogen citrate 2 g, glucose 20 g, Tween 80 1 ml, sodium acetate 5 g, dipotassium hydrogen phosphate 2 g, magnesium sulfate 0.58 g, manganese sulfate 0.25 g, distilled water 1000 ml, 6.2～6.6,121 ℃ of moist heat sterilization 15min of pH.
2, the preparation of saccharomycopsis fibuligera bacterium bacterium liquid
The saccharomycopsis fibuligera bacterium that 4 ℃ of inclined-planes are preserved is connected on YEPD liquid tube culture medium and activates, at 30 ℃ with 220rmin
-1shaken cultivation 24h, cultivates seed as one-level; With 1% inoculum concentration, be connected in YEPD triangular flask fluid nutrient medium again, at 30 ℃ with 220rmin
-1shaken cultivation 24h.YEPD culture medium: dusty yeast 10 g, peptone 20 g, glucose 20 g, distilled water 1000 ml, 6.0,115 ℃ of moist heat sterilization 20min of pH.
3, the preparation of bacillus subtilis bacterium liquid
The bacillus subtilis that 4 ℃ of inclined-planes are preserved is connected on beef extract-peptone liquid tube culture medium and activates, at 32 ℃ with 220rmin
-1shaken cultivation is cultivated 24h, as one-level, cultivates seed; With 1% inoculum concentration, be connected in beef extract-peptone triangular flask fluid nutrient medium again, at 32 ℃ with 220rmin
-1shaken cultivation 24h.Beef-protein medium: beef-protein medium: beef extract 0.5 g, peptone 15 g, sodium chloride 5 g, glucose 20 g, distilled water 1000 ml, 7.0～7.2,115 ℃ of moist heat sterilization 20min of pH.
4, the preparation of black-koji mould liquid
The aspergillus niger that 4 ℃ of inclined-planes are preserved is connected on Cha Shi liquid tube culture medium and activates, at 28 ℃ with 220rmin
-1shaken cultivation is cultivated 36h, as one-level, cultivates seed; With 1% inoculum concentration, be connected in Cha Shi triangular flask fluid nutrient medium again, at 28 ℃ with 220rmin
-1shaken cultivation 36h.Czapek's medium: sodium nitrate 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5 g, potassium chloride 0.5 g, ferrous sulfate 0.01 g, sucrose 30 g, distilled water 1000 ml, pH nature, 121 ℃ of moist heat sterilization 20min.
The present invention is mainly that application potato residue is raw material, dry jujube powder and defatted rice bran are auxiliary material, add on this basis reagent: urea, ammonium sulfate, potassium dihydrogen phosphate and magnesium sulfate, be mixed to get fermentation substrate, the combined fermentation bacterial classification bacterium liquid being mixed into through the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum, first aerobic fermentation, then anaerobic fermentation is prepared from.Its principle is: potato residue, defatted rice bran and dry jujube powder contain enough starch and crude fibre and a small amount of true albumen; Urea, ammonium sulfate contain the available a large amount of inorganic nitrogens of microorganism; Magnesium sulfate, potassium dihydrogen phosphate can promote some beneficial microbe growth; Above-mentioned three class material action compensatings, application simultaneously can produce Overlay.
Application saccharomycopsis fibuligera can improve fermentation substrate true protein content; Bacillus subtilis can produce protease, improves protein utilization rate; Aspergillus niger can eccrine fiber element enzyme degradation of fibers, thereby also can secrete pectase decompose pectin; Lactobacillus plantarum can produce lactic acid, improves feed local flavor; Fermentation substrate is after aerobic, anaerobic fermentation are processed, and true protein content is 13.24%, and total amount has improved 36.93%, and crude fiber content is 24.13%, and total amount has reduced by 41.00%, and has sweet-smelling smell.
The beneficial effect that adopts technique scheme to produce is:
(1) the present invention can increase the true protein content of fermentation substrate, reduces crude fibre amount, improves feed palatability and digestive utilization ratio, improves potato residues nutritive value comprehensively, has economy and environment double benefit; After application the inventive method is processed fermentation substrate, true protein content is 13.24%, and total amount has improved 36.93%, and crude fiber content is 24.13%, and total amount has reduced by 41.00%, and has sweet-smelling smell.
(2) product of the present invention substitutes wheat bran by 15% part by weight, can improve growing and fattening pig feed intake, daily gain, reduces feedstuff-meat ratio.
(3) the present invention is pure green product, without any side effects, does not produce incompatibility with other raw materials.
The specific embodiment
Potato residue is purchased from Kangbao County, Zhangjiakou moral along starch factory, water content≤14wt%, and market is on sale.
Embodiment 1
Adopt supplementary material and the reagent of following weight composition: 160 grams of potato residues, 20 grams, dry jujube powder, 20 grams of defatted rice brans, 3 grams, urea, 3 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: after above-mentioned supplementary material and reagent are weighed, input pulverizer is pulverized, dropping into mixer mixes again, make fermentation substrate, then take the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum as combined fermentation bacterial classification bacterium liquid, wherein the bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is 1 × 10
6individual/ml, the ratio that combined fermentation bacterial classification bacterium liquid is 3:3:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes, fermentation substrate evenly mixes by the weight ratio of 1:1 with combined fermentation bacterial classification bacterium liquid, 28-30 ℃ of aerobic fermentation 48 hours, then anaerobic fermentation 24 hours, dry to water content≤14wt% for 70-80 ℃, pulverize.
Embodiment 2
Adopt supplementary material and the reagent of following weight composition: 160 grams of potato residues, 20 grams, dry jujube powder, 20 grams of defatted rice brans, 3 grams, urea, 1.5 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 1:2:2:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.2 with combined fermentation bacterial classification bacterium liquid.
Embodiment 3
Adopt supplementary material and the reagent of following weight composition: 150 grams of potato residues, 30 grams, dry jujube powder, 20 grams of defatted rice brans, 3 grams, urea, 3 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 1:1:1:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.1 with combined fermentation bacterial classification bacterium liquid.
Embodiment 4
Adopt supplementary material and the reagent of following weight composition: 150 grams of potato residues, 30 grams, dry jujube powder, 20 grams of defatted rice brans, 3 grams, urea, 1.5 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 2:1:2:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.05 with combined fermentation bacterial classification bacterium liquid.
Embodiment 5
Adopt supplementary material and the reagent of following weight composition: 160 grams of potato residues, 20 grams, dry jujube powder, 20 grams of defatted rice brans, 1.5 grams, urea, 1.5 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 1:3:3:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.15 with combined fermentation bacterial classification bacterium liquid.
Embodiment 6
Adopt supplementary material and the reagent of following weight composition: 150 grams of potato residues, 30 grams, dry jujube powder, 20 grams of defatted rice brans, 1.5 grams, urea, 1.5 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 3:1:3:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes;
Embodiment 7
Adopt supplementary material and the reagent of following weight composition: 160 grams of potato residues, 30 grams, dry jujube powder, 10 grams of defatted rice brans, 2 grams, urea, 1.8 grams, ammonium sulfate, 1 gram of potassium dihydrogen phosphate, 0.8 gram, magnesium sulfate.
Preparation method: be to be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 2:2:3:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes with the difference of embodiment 1.
Embodiment 8
Adopt supplementary material and the reagent of following weight composition: 150 grams of potato residues, 20 grams, dry jujube powder, 30 grams of defatted rice brans, 2.5 grams, urea, 2 grams, ammonium sulfate, 1.1 grams of potassium dihydrogen phosphates, 1.2 grams, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 2:3:1:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.2 with combined fermentation bacterial classification bacterium liquid.
Embodiment 9
Adopt supplementary material and the reagent of following weight composition: 156 grams of potato residues, 24 grams, dry jujube powder, 20 grams of defatted rice brans, 1.7 grams, urea, 2.5 grams, ammonium sulfate, 1.3 grams of potassium dihydrogen phosphates, 0.9 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 3:3:2:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.1 with combined fermentation bacterial classification bacterium liquid, 28-30 ℃ of aerobic fermentation 60 hours, then anaerobic fermentation 48 hours.
Embodiment 10
Adopt supplementary material and the reagent of following weight composition: 152 grams of potato residues, 28 grams, dry jujube powder, 20 grams of defatted rice brans, 2.2 grams, urea, 2.7 grams, ammonium sulfate, 1.4 grams of potassium dihydrogen phosphates, 1.1 grams, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 3:2:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.15 with combined fermentation bacterial classification bacterium liquid, 28-30 ℃ of aerobic fermentation 72 hours, then anaerobic fermentation 36 hours.
Its using method of potato residues fermented feed of the present invention is: with this product, at growing-finishing pigs diets moderate, substitute 15% wheat bran, other raw materials are constant.
Experimental example:
This potato residues fermented feed affects experimental design to growing-finishing pig production performance
1.1 experimental animals and grouping
Select age in days to differ to be no more than 120 of 30 kilograms of identical left and right pigs of 7 days, kind, the principle identical according to genetic background, body weight is close, sex ratio is consistent is divided into 2 processing at random, 6 repetitions are established in every processing, 10 of every repetitions, and between each group, starting weight difference is not remarkable.
Control group: the basal diet of feeding; Test group: feed and substitute the test daily ration of equivalent wheat bran with 15% potato residues fermented feed.
1.2 test daily ration and Treatment Design
With reference to NRC(1998) growing-finishing pig nutrition requirements in conjunction with production practices preparations basal diet.
Table 1 Diet Formula and trophic level
1.3 feeding and management
Test is carried out in same closed pig house, raises on the net, and humiture is suitable; Free choice feeding and drinking-water.Immunity disinfectant program is undertaken by pig farm conventional method.Test is responsible for by special messenger, in strict accordance with test requirements document, manages.Feeding and management is carried out with reference to the feeding and management rules of this product boar.Test period 40 days.
1.4. test method
When on-test and end, take individuality as unit, all test pig are carried out to empty stomach and weigh, record in detail body weight; In process of the test to repeat recording in detail feed intake as unit; Record in detail pig death and superseded situation, while there is dead naughty pig, expect and weigh for timely section.
1.5 detect index
The average daily gain of growth performance index, average daily ingestion amount and feedstuff-meat ratio.
1.6 data processings and statistical analysis technique
Data acquisition carries out variance analysis with the ANOVA in SPSS13.0 statistical software; Pat everywhere between mean and carry out significance of difference comparison by Duncan method, result of the test data represent with mean+SD.
2. results and analysis
The impact of table 2 on growing-finishing pig production performance
Note: not remarkable (P>0.05) of the identical person's difference of colleague's shoulder mark lowercase, the different persons of colleague's shoulder mark represent significant difference (P<0.05).
As can be seen from Table 2, test group off-test weight ratio control group has improved 3.42%(P<0.05); Average daily gain has improved 7.12%(P<0.05 than control group); Average daily ingestion amount has improved 3.11%(P>0.05 than control group); Feedstuff-meat ratio reduces 3.82%(P>0.05 than control group).
3. conclusion
By result of the test, can be obtained to draw a conclusion:
In growing and fattening pig feed, with fermenting potato slag equivalent substitution wheat bran, can significantly improve growing and fattening daily gain in pigs, feed intake, feedstuff-meat ratio are also improved to trend;
Because this product is safe green feedstuff, and cost is significantly lower than substitute-wheat bran, and therefore produce market has a extensive future.
Claims (4)
1. a potato residues fermented feed, it is characterized in that: take potato residue 75-80wt%, dry jujube powder 10-15wt% and defatted rice bran 5-15wt% as supplementary material, on this supplementary material basis, add reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, be mixed to get fermentation substrate, with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum, be mixed into combined fermentation bacterial classification bacterium liquid, first aerobic fermentation, then anaerobic fermentation is prepared from; Water content≤the 14wt% of described potato residue; The weight ratio of fermentation substrate and combined fermentation bacterial classification bacterium liquid is 1:1-1.2; The bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is 1 × 10
6individual/ml, the ratio that combined fermentation bacterial classification bacterium liquid is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes.
2. potato residues fermented feed according to claim 1, is characterized in that the ratio that described combined fermentation bacterial classification bacterium liquid is 3:3:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes.
3. a preparation method for potato residues fermented feed as claimed in claim 1 or 2, is characterized in that, comprises the following steps:
(1) take potato residue 75-80wt%, dry jujube powder 10-15wt% and defatted rice bran 5-15wt% as supplementary material, on this supplementary material basis, add reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, mix, make fermentation substrate; Water content≤the 14wt% of described potato residue;
(2) above-mentioned fermentation substrate is mixed into combined fermentation bacterial classification bacterium liquid with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum, fermentation substrate evenly mixes by the weight ratio of 1:1-1.2 with combined fermentation bacterial classification bacterium liquid, 28-30 ℃ of aerobic fermentation 48-72 hour, then anaerobic fermentation 24-48 hour, dries and pulverizes; The bacterial concentration of described bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is 1 × 10
6individual/ml, the ratio that combined fermentation bacterial classification bacterium liquid is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes.
4. the preparation method of potato residues fermented feed according to claim 3, is characterized in that the ratio that described combined fermentation bacterial classification bacterium liquid is 3:3:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum according to volume ratio mixes.
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| CN105961828A (en) * | 2016-03-22 | 2016-09-28 | 安徽旺旺禽业科技有限公司 | Ganoderma lucidum feed for broiler chicken and preparation method thereof |
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| CN110269145A (en) * | 2019-08-01 | 2019-09-24 | 哈尔滨工业大学 | The process of potato starch industrial by-products fermenting and producing cell protein |
| CN110269145B (en) * | 2019-08-01 | 2023-06-09 | 哈尔滨工业大学 | Process method for producing cellular protein by fermenting potato starch industrial byproducts |
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| CN114766596A (en) * | 2022-05-09 | 2022-07-22 | 广东大泽农生物科技股份有限公司 | Degradation method of coix seed bran and Chinese gall residue, application of degradation method and biological fermentation feed |
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