CN104263783A - Method for preparing protein peptide through waste water produced from potato starch producing - Google Patents
Method for preparing protein peptide through waste water produced from potato starch producing Download PDFInfo
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- CN104263783A CN104263783A CN201410525789.2A CN201410525789A CN104263783A CN 104263783 A CN104263783 A CN 104263783A CN 201410525789 A CN201410525789 A CN 201410525789A CN 104263783 A CN104263783 A CN 104263783A
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Abstract
The invention provides a method for preparing protein peptide through waste water produced from potato starch producing. The method comprises the following steps: taking waste water produced from production, heating to 50 DEG C, adjusting the pH value to be 8.5, performing heat preservation for 3.5 h, leaving the waste water to stand for 1 h, removing 80% of supernatant, adjusting the PH value to 6.5-7.0, stirring, sterilizing for 20 minutes at 115 DEG C, cooling to 30 DEG C, inoculating 10% of liquid bacillus subtilis strain, stirring, and fermenting for 68 hours at 28-30 DEG C and at the rotation speed of 150 rpm; sterilizing for 15 minutes at 115 DEG C, performing centrifugal separation, re-concentrating the supernatant until the solid accounts for 25-30%, and performing spray drying. The prepared protein peptide is applicable to the feed industry.
Description
Technical field
The present invention relates to the working method of protein, specifically a kind of method utilizing waste water produced in potato starch manufacturing to prepare potato protein peptide.
Background technology
In yam starch production process, a large amount of waste water and waste residue are discharged, these waste water contain a large amount of organism, the waste water of high density, high pollution, wherein COD can reach more than 50000, and triumphant formula nitrogen reaches about 3500, if do not processed, both wasted resource, again serious pollution was caused to environment.Because in sewage, nitrogen content is higher, be difficult to process, namely enable process is up to standard, processing costs also can be very high, and the installations and facilities investment of sewage disposal can be very large, and utilize the feature that its organism, especially protein content are higher, carrying out fully utilizing is more positive settling mode.
Summary of the invention
The invention provides a kind of method utilizing waste water produced in potato starch manufacturing to prepare protein peptide.
The object of the present invention is achieved like this: the waste water getting yam starch centrifugal station, is heated to 50 DEG C, and the phosphoric acid solution with 15% regulates pH to 8.5, insulation 3.5h, leaves standstill 1h, adopts siphon method to discard the supernatant liquor of 80%, adjust pH value to 6.5-7.0, stir, 115 DEG C of sterilizings 20 minutes, are cooled to 30 DEG C, by 10% access subtilis liquid seeds of slurries total amount, stir, 28 DEG C-30 DEG C, under rotating speed 150rpm condition, ferment 68 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, by supernatant liquor reconcentration to solid substance 25-30%, spraying dry i.e. obtained potato protein peptide.
The cultural method of described subtilis liquid seeds is: take 5g peptone, 4g glucose, 0.1g potassium primary phosphate, 0.05g magnesium sulfate, add 90ml water, stir, 121 DEG C of sterilizings 30 minutes, be cooled to 30 DEG C, access subtilis inclined-plane seed, stirs, 28 DEG C-30 DEG C, under rotating speed 200rpm condition, cultivate 24 hours.
The present invention take waste water produced in potato starch manufacturing as raw material, and by the method for fermentable, the potato protein in waste water is converted into potato protein peptide, it can be used as the added ingredients of feed.Protein peptide has higher biological activity and nutritive value than the amino acid of equivalent, small-molecule peptide and amino acid almost all can be absorbed and used by the Epithelium of intestinal villus cell of animal completely, enter into blood, then each position of organism is transported to, protein just can be absorbed and used after then having to pass through digestion, therefore peptide class is by the total free aminoacids of animal absorption rate faster than equivalent, greatly faster than the protein of equivalent.Potato protein peptide is applied to feedstuff industry and can improves cultivation efficiency, reduce aquaculture cost.
The present invention utilizes waste water produced in potato starch manufacturing production potato protein peptide, reduces the organic content in waste water, greatly reduces the load of wastewater treatment, be beneficial to and realize qualified discharge, decrease environmental pollution.Meanwhile, improve comprehensive process level and the economic benefit of Potato ring rot bacteria.
Four, specific embodiment
Embodiment one
The cultivation of subtilis liquid seeds: take 5g peptone, 4g glucose, 0.1g potassium primary phosphate, 0.05g magnesium sulfate, add 90mL water, stir, 121 DEG C of sterilizings 30 minutes, are cooled to 30 DEG C, access subtilis inclined-plane seed, stir, 28 DEG C-30 DEG C, under rotating speed 200rpm condition, cultivate 24 hours, detect qualified after, be subtilis liquid seeds.
The preparation of fermention medium and fermentation process are: the waste water 1000g taking yam starch centrifugal station, is heated to 50 DEG C, and the phosphoric acid solution with 15% regulates pH to 8.5, insulation 3.5h, leaves standstill 1h, adopts siphon method to discard 800g supernatant liquor, obtain 200g slurries, adjust pH value to 6.5-7.0, stir, 115 DEG C of sterilizings 20 minutes, be cooled to 30 DEG C, access 20mL subtilis liquid seeds, stirs, 28 DEG C-30 DEG C, under rotating speed 150rpm condition, ferment 68 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, by supernatant liquor reconcentration to solid substance 28%, spraying dry i.e. obtained potato protein peptide.
Embodiment two
The cultivation of liquid seeds: take 20g peptone, then claim 16g glucose, 0.4g potassium primary phosphate, 0.2g magnesium sulfate, adds 360mL water, stirs, 121 DEG C of sterilizings 30 minutes, be cooled to 30 DEG C, access subtilis inclined-plane seed, stirs, 28 DEG C-30 DEG C, under rotating speed 200rpm condition, cultivate 24 hours, detect qualified after, be subtilis liquid seeds.
The preparation method of potato protein peptide: the waste water 10kg taking yam starch centrifugal station, is heated to 50 DEG C, the phosphoric acid solution with 15% regulates pH to 8.5, insulation 3.5h, leaves standstill 1h, adopts siphon method to discard 8kg supernatant liquor, obtain 2kg slurries, adjust pH value to 6.5-7.0, stir, 115 DEG C of sterilizings 20 minutes, be cooled to 30 DEG C, access 200mL subtilis liquid seeds, stirs, 28 DEG C-30 DEG C, under rotating speed 150rpm condition, ferment 68 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, by supernatant liquor reconcentration to solid substance 25%, spraying dry i.e. obtained potato protein peptide.
Claims (1)
1. the method utilizing waste water produced in potato starch manufacturing to prepare protein peptide, it is characterized in that: the waste water getting yam starch centrifugal station, be heated to 50 DEG C, phosphoric acid solution with 15% regulates pH to 8.5, insulation 3.5h, leave standstill 1h, adopt siphon method to discard the supernatant liquor of 80%, adjust pH value to 6.5-7.0, stir, 115 DEG C of sterilizings 20 minutes, be cooled to 30 DEG C, by 10% access subtilis liquid seeds of slurries total amount, stir, 28 DEG C-30 DEG C, under rotating speed 150rpm condition, ferment 68 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, by supernatant liquor reconcentration to solid substance 25-30%, spraying dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410525789.2A CN104263783A (en) | 2014-10-09 | 2014-10-09 | Method for preparing protein peptide through waste water produced from potato starch producing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410525789.2A CN104263783A (en) | 2014-10-09 | 2014-10-09 | Method for preparing protein peptide through waste water produced from potato starch producing |
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| CN104263783A true CN104263783A (en) | 2015-01-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410525789.2A Pending CN104263783A (en) | 2014-10-09 | 2014-10-09 | Method for preparing protein peptide through waste water produced from potato starch producing |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110643654A (en) * | 2019-10-17 | 2020-01-03 | 浙江大学 | Method for producing polysaccharide by fermenting starch wastewater |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102127513A (en) * | 2010-01-12 | 2011-07-20 | 北京联合大学 | Method for culturing Bacillus subtilis by using potato starch wastewater |
| CN102210383A (en) * | 2011-04-08 | 2011-10-12 | 甘肃圣大方舟马铃薯变性淀粉有限公司 | Method for preparing mycoprotein feed from waste water and waste residues in processing process of potatoes |
| CN103141667A (en) * | 2013-04-08 | 2013-06-12 | 黑龙江省轻工科学研究院 | Method for producing corn protein peptide used for feeding by spent grains liquid of corn alcohol |
| CN103749966A (en) * | 2014-01-27 | 2014-04-30 | 河北农业大学 | Potato pulp fermented feed and preparation method thereof |
-
2014
- 2014-10-09 CN CN201410525789.2A patent/CN104263783A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102127513A (en) * | 2010-01-12 | 2011-07-20 | 北京联合大学 | Method for culturing Bacillus subtilis by using potato starch wastewater |
| CN102210383A (en) * | 2011-04-08 | 2011-10-12 | 甘肃圣大方舟马铃薯变性淀粉有限公司 | Method for preparing mycoprotein feed from waste water and waste residues in processing process of potatoes |
| CN103141667A (en) * | 2013-04-08 | 2013-06-12 | 黑龙江省轻工科学研究院 | Method for producing corn protein peptide used for feeding by spent grains liquid of corn alcohol |
| CN103749966A (en) * | 2014-01-27 | 2014-04-30 | 河北农业大学 | Potato pulp fermented feed and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 万俊杰等: "食品废水培养枯草芽孢杆菌产絮凝剂及固定化吸附Cu~(2+)研究", 《安徽农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110643654A (en) * | 2019-10-17 | 2020-01-03 | 浙江大学 | Method for producing polysaccharide by fermenting starch wastewater |
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