CN104263784A - Method for preparing corn protein peptide through waste water produced from corn starch producing - Google Patents
Method for preparing corn protein peptide through waste water produced from corn starch producing Download PDFInfo
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- CN104263784A CN104263784A CN201410531407.7A CN201410531407A CN104263784A CN 104263784 A CN104263784 A CN 104263784A CN 201410531407 A CN201410531407 A CN 201410531407A CN 104263784 A CN104263784 A CN 104263784A
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Abstract
The invention provides a method for preparing corn protein peptide through waste water produced from corn starch producing. The method comprises the following steps: taking the waste water produced from corn starch producing, adding 0.06% of sodium sulfite, heating to 40 DEG C, preserving heat and settling for 8 h, removing 80% of supernatant by a siphon method, adjusting the PH value to 6.5-7.0, uniformly stirring, sterilizing for 30 minutes at 121 DEG C, cooling to 30 DEG C, inoculating liquid bacillus subtilis strain accounting for 8% of the total amount of a slurry, stirring, and fermenting for 72 hours at 28-30 DEG C and at the rotation speed of 150 rpm; sterilizing for 15 minutes at 115 DEG C, performing centrifugal separation, re-concentrating the supernatant until the solid accounts for 25-30%, and performing spray drying to obtain the corn protein peptide. The prepared corn protein peptide is applicable to the feed industry.
Description
Technical field
The present invention relates to a kind of working method of protein, specifically a kind of method utilizing production of corn starch waste water to prepare corn protein peptide.
Background technology
When taking corn as raw material production starch, can produce a large amount of containing the organic compound of high density and the waste water of suspended substance, it is mainly derived from the operations such as the immersion of corn, germ separation, fiber wash and dehydration.Often produce 1 ton of W-Gum and about produce 15-20 ton waste water, waste water generation is comparatively large, mainly starch-containing, albumen and degraded intermediate etc. thereof.If waste water directly discharges, because it contains the large amount of organic such as protein, carbohydrate, not only serious pollution is caused to environment, waste resource, add the comprehensive cost of Starch Production; If carry out wastewater treatment, because its organism nitrogen content is higher, processing costs also can be very high, and the installations and facilities investment of sewage disposal can be very large.Utilize the feature that corn starch wastewater organism, especially protein content are higher, fully utilizing waste water is more positive settling mode.
Summary of the invention
The invention provides a kind of method utilizing production of corn starch waste water to prepare corn protein peptide.
The object of the present invention is achieved like this: extracting corn starch factory effluent, adds the S-WAT of 0.06%, is heated to 40 DEG C, heat preservation settlement 8h, adopt siphon method to discard the supernatant liquor of 80%, adjust pH value to 6.5-7.0, stir, 121 DEG C of sterilizings 30 minutes, be cooled to 30 DEG C, by 8% access subtilis liquid seeds of slurries total amount, stir, 28 DEG C-30 DEG C, under rotating speed 150rpm condition, ferment 72 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, by supernatant liquor reconcentration to solid substance 25-30%, spraying dry i.e. obtained corn protein peptide.
The cultural method of described subtilis liquid seeds is: take 5g peptone, 4g glucose, 0.1g potassium primary phosphate, 0.05g magnesium sulfate, add 90ml water, stir, 121 DEG C of sterilizings 30 minutes, be cooled to 30 DEG C, access subtilis inclined-plane seed, stirs, 28 DEG C-30 DEG C, under rotating speed 180rpm condition, cultivate 24 hours.
The albumen that corn contains is based on prolamine, and prolamine is not soluble in water, is therefore not easily absorbed by organism.The present invention is for raw material with production of corn starch waste water, by the method for fermentable, the prolamine not soluble in water in waste water is converted into small molecules, corn protein peptide soluble in water, substantially increase the trophicity of zein, functional, expand its Application Areas and use range.Protein peptide has higher biological activity and nutritive value than the amino acid of equivalent, and it is by the total free aminoacids of animal absorption rate faster than equivalent, greatly faster than the protein of equivalent.Corn protein peptide contains 15 kinds of inorganic salt such as 18 seed amino acid such as L-glutamic acid, leucine, vitamin A, potassium, phosphorus, magnesium, sulphur and the exclusive corn yellow OB of corn, can be used as the added ingredients of feed, also as foodstuff additive.Corn protein peptide is applied to feedstuff industry and can improves cultivation efficiency, reduce aquaculture cost.
The present invention utilizes production of corn starch waste water to produce corn protein peptide, reduces the organic content in waste water, greatly reduces the load of wastewater treatment, be beneficial to and realize qualified discharge, decrease environmental pollution.Meanwhile, improve comprehensive process level and the economic benefit of W-Gum industry.
Four, specific embodiment
Embodiment one
The cultivation of liquid seeds: take 5g peptone, 4g glucose, 0.1g potassium primary phosphate, 0.05g magnesium sulfate, add 90mL water, stir, 121 DEG C of sterilizings 30 minutes, are cooled to 30 DEG C, access subtilis inclined-plane seed, stir, 28 DEG C-30 DEG C, under rotating speed 180rpm condition, cultivate 24 hours, detect qualified after, be subtilis liquid seeds.
The preparation method of corn protein peptide: take production of corn starch waste water 1000g, add 0.6g S-WAT, be heated to 40 DEG C, heat preservation settlement 8h, adopts siphon method to discard 800g supernatant liquor, stays 200g slurries, adjust pH value to 6.5-7.0, stir, 121 DEG C of sterilizings 30 minutes, are cooled to 30 DEG C, access 16mL subtilis liquid seeds, stir, 28 DEG C-30 DEG C, under rotating speed 150rpm condition, ferment 72 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, by supernatant liquor reconcentration to solid substance 26%, spraying dry i.e. obtained corn protein peptide.
Embodiment two
The cultivation of liquid seeds: take 10g peptone, then claim 8g glucose, 0.2g potassium primary phosphate, 0.2g magnesium sulfate, adds 180mL water, stirs, 121 DEG C of sterilizings 30 minutes, be cooled to 30 DEG C, access subtilis inclined-plane seed, stirs, 28 DEG C-30 DEG C, under rotating speed 200rpm condition, cultivate 24 hours, detect qualified after, be subtilis liquid seeds.
The preparation method of corn protein peptide: take production of corn starch waste water 5kg, add 3g S-WAT, be heated to 40 DEG C, heat preservation settlement 8h, adopts siphon method to discard the supernatant liquor of 4kg, obtains 1kg slurries, adjust pH value to 6.5-7.0, stir, 121 DEG C of sterilizings 30 minutes, are cooled to 30 DEG C, access 80mL subtilis liquid seeds, stir, 28 DEG C-30 DEG C, under rotating speed 180rpm condition, ferment 72 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, by supernatant liquor reconcentration to solid substance 28%, spraying dry i.e. obtained corn protein peptide.
Claims (1)
1. the invention provides a kind of method utilizing production of corn starch waste water to prepare corn protein peptide, its feature is as follows: extracting corn starch factory effluent, add the S-WAT of 0.06%, be heated to 40 DEG C, heat preservation settlement 8h, siphon method is adopted to discard the supernatant liquor of 80%, adjust pH value to 6.5-7.0, stir, 121 DEG C of sterilizings 30 minutes, are cooled to 30 DEG C, by 8% access subtilis liquid seeds of slurries total amount, stir, 28 DEG C-30 DEG C, under rotating speed 150rpm condition, ferment 72 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, by supernatant liquor reconcentration to solid substance 25-30%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410531407.7A CN104263784A (en) | 2014-10-11 | 2014-10-11 | Method for preparing corn protein peptide through waste water produced from corn starch producing |
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| CN201410531407.7A CN104263784A (en) | 2014-10-11 | 2014-10-11 | Method for preparing corn protein peptide through waste water produced from corn starch producing |
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| CN104263784A true CN104263784A (en) | 2015-01-07 |
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| CN201410531407.7A Pending CN104263784A (en) | 2014-10-11 | 2014-10-11 | Method for preparing corn protein peptide through waste water produced from corn starch producing |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994938A (en) * | 2016-05-23 | 2016-10-12 | 吉林省农业科学院 | Production method of compound bacterium solid-state fermented maize protein meal |
| CN107217018A (en) * | 2017-06-19 | 2017-09-29 | 武汉轻工大学 | Resistance bacillus subtilis and its application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101503716A (en) * | 2008-10-13 | 2009-08-12 | 哈尔滨商业大学 | Method for preparing poly-gamma-glutamic acid by fermenting maize raw material Bacillus subtilis |
| CN101676242A (en) * | 2008-09-17 | 2010-03-24 | 山东福田药业有限公司 | Manufacturing method for producing anti-disease microbial fertilizer by utilizing corncob waste residue |
| CN103141667A (en) * | 2013-04-08 | 2013-06-12 | 黑龙江省轻工科学研究院 | Method for producing corn protein peptide used for feeding by spent grains liquid of corn alcohol |
-
2014
- 2014-10-11 CN CN201410531407.7A patent/CN104263784A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101676242A (en) * | 2008-09-17 | 2010-03-24 | 山东福田药业有限公司 | Manufacturing method for producing anti-disease microbial fertilizer by utilizing corncob waste residue |
| CN101503716A (en) * | 2008-10-13 | 2009-08-12 | 哈尔滨商业大学 | Method for preparing poly-gamma-glutamic acid by fermenting maize raw material Bacillus subtilis |
| CN103141667A (en) * | 2013-04-08 | 2013-06-12 | 黑龙江省轻工科学研究院 | Method for producing corn protein peptide used for feeding by spent grains liquid of corn alcohol |
Non-Patent Citations (1)
| Title |
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| 吴泽柱等: "玉米蛋白肽的生产加工研究现状", 《中国食品添加剂》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994938A (en) * | 2016-05-23 | 2016-10-12 | 吉林省农业科学院 | Production method of compound bacterium solid-state fermented maize protein meal |
| CN107217018A (en) * | 2017-06-19 | 2017-09-29 | 武汉轻工大学 | Resistance bacillus subtilis and its application |
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Application publication date: 20150107 |