CN103735710A - Traditional Chinese medicine composition for treating recessive mastitis of dairy cows and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating recessive mastitis of dairy cows and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for treating recessive mastitis of dairy cows and a preparation method thereof. The traditional Chinese medicine composition comprises the following raw herbal materials: the seed of cowherb, astragalus membranaceus, Chinese angelica, codonopsis pilosula, incised spleenwort, fructus liquidambaris, babysbreath, uniflower swisscentaury root, radix paeoniae alba, derris eriocarpa how, caulis clematidis armandii, peach grass, delavay schefflera root and common adenostema herb. The preparation method comprises the following steps of: weighing all the raw herbal materials according to parts by weight, crushing the raw herbal materials in a crusher, and sieving through a 80-120-mesh sieve to obtain powder. The traditional Chinese medicine composition for treating recessive mastitis of dairy cows and the preparation method thereof disclosed by the invention have the beneficial effects that the traditional Chinese medicine composition disclosed by the invention has the effects of clearing heat, removing toxicity, eliminating stasis, dispelling swelling and relieving pain; after being taken orally and used externally, the traditional Chinese medicine composition is penetrated into bodies through skin, therefore, the therapeutic effect is increased; the curative effect is improved; the traditional Chinese medicine composition is used for treating internally and externally, and resolves the current problems and eliminates the root causes; the exterior is nourished by the interior; orally taken and externally applied medicines are promoted with each other; the auto-immunity is increased; side effects to dairy cows cannot be generated; the traditional Chinese medicine composition disclosed by the invention is safe and effective.
Description
Technical field
The present invention relates to contain the beast medicinal preparation that derives from plant, animal or mineral component, particularly a kind of Chinese medicine composition that is used for the treatment of bovine subclinical mastitis and preparation method thereof.
Background technology
Mastitis is one of modal disease of milch cow, is also the disease to milch cow disserve to produce maximum.Mastitis is that mammary gland tissue is subject to physics, chemistry, microorganism and stimulates a kind of inflammatory occurring to change.Be characterized in that Ruzhong somatic cell increases, mammary gland tissue generation pathological change, the character quality of breast occurs abnormal.
The harm mastitis average attack rate of latent mammitis is up to 40%～65%, and wherein the average attack rate of clinic mastitis is 2%～3%, and the average attack rate of latent mammitis is 38%～62%.The sick milch cow of mastitis not only milk yield declines, milk products degrading quality, and owing to discarding the increase of milk, the increasing of medical care expenses and eliminating too early etc. causes huge economic loss to dairy.
Although latent mammitis does not have manifest symptom clinically, caused the decline of milk yield and milk-quality.There is larger variation in the newborn nutritional labeling of sick cattle, and contains a large amount of invasive organisms, makes milk be easy to become sour rotten, affects again the healthy and safe of the nutritive value of milk product and people.Raising so sick cattle should increase feeding and management cost simultaneously, also can be transformed into clinic mastitis and stop giving milk when serious, to such an extent as to make milch cow have to eliminate, processes, causes larger economic loss; Latent mammitis can also cause the infection of reproductive system and ill, and suffers from the milch cow of latent mammitis, post-partum estrus time lengthening, and conception rate declines etc.Latent mammitis is pathogen carrier, can infect other cattle.Most of clinical mastitiss originate in latent mammitis, therefore, to latent mammitis, take necessary measure more important than the clinical mastitis of simple therapy, are also the important contents that milk cattle cultivating is managed.
Cause the main cause microorganism reason of bovine subclinical mastitis: the main reason that causes mastitis is the infection of pathogen, be mostly staphylococcus aureus and streptococcus, other also have escherichia coli, bacillus pyocyaneus, suppurative corynebacterium, necrobacillus etc.These pathogenic bacterias are generally invaded and to cause morbidity through papillary duct, also have plenty of the intrusion breast such as the uterus of falling ill through wound or gastrointestinal tract and fall ill.Environmental factors: as many in cowshed dust, unclean, do not sterilize; Cattle bed is moist, rinses not in time again, sterilizes; Sports ground is muddy, straw in a mattress is unclean; Milch cow sanitary condition is poor, and breast is by mud scholar, fecal pollution; Before milking, do not wash one's hands, do not clean nipple, while cleaning breast, one barrel of water of several cattle, a towel are cleaned a lot of cattle; Vacuum pump actuator is unclean, or often replacing of the rubber tube on milker, or cleaning milker does not add any disinfectant; The sick cattle Excreta of mastitis arbitrarily drains, three milk be arbitrarily extruded on cattle bed, contaminated environment etc.Traumatic factor: the unskilled or misoperation of various wounds and Milking Methods And Techniques, as excessive in milking machine negative pressure, milk middle twitch overlong time, excessive velocities, even the in the situation that of valve-off not, leave behind by force breast cup or lead the reasons such as latex dust improper use, and make papillary duct or newborn pond mucosa injury etc., can cause latent mammitis.Autogenous infection: as diseases such as puerperal septiemia, acute endometritis, tuberculosis, brucellosis, gastroenteritiss, the generation of most latent mammitises with local response.Latent mammitis also can be caused by the transfer of the diseases such as traumatic cervicitis, pyopericarditis, blood vessel embolism internal organs organ pipe.Feeding and management factor: diet nutrient is unbalanced, for high yield cow, the daily ration of high-energy, high protein is conducive to protection and improves milk yield, has also increased the load of breast simultaneously, the resistance of body is reduced, thereby cause mastitis.Stress: as bad climate (comprising severe cold, heat etc.), scaring, feedstuff moldy metamorphism etc., they can affect the normal physiological function of milch cow to a certain extent, cause mastitis morbidity to increase.Veterinary operates the milk cattle infected mastitis that also can make lack of standardization.High parity, older milch cow easily suffer from latent mammitis.The main manifestations of the sick cattle of latent mammitis and diagnosis main manifestations: latent mammitis does not generally show clinical symptoms, but it has distinctive syndrome.As milk yield reduces increasingly, the composition generation significant change containing in milk, histiocyte occurs in a large number as neutrophil, epithelial cell etc., and can retain the long duration in milk.Milk becomes alkalescence by normal faintly acid, and the quality of breast reduces, and easily becomes sour rotten.Latent mammitis, as treatment not in time, may transfer clinical mastitis to.
Summary of the invention
The object of the present invention is to provide a kind of instant effect, therapeutic effect is good and side effect is little is used for the treatment of Chinese medicine composition of bovine subclinical mastitis and preparation method thereof.
In order to realize foregoing invention object, the invention provides a kind of Chinese medicine composition that is used for the treatment of bovine subclinical mastitis, described Chinese medicine composition comprises following raw medicinal material: Semen Vaccariae, the Radix Astragali, Radix Angelicae Sinensis, Radix Codonopsis, Herba Asplenii Incisi, Fructus Liquidambaris, Herba Stellariae Neglectae, Radix Rhapontici, Radix Paeoniae Alba, pungent ginseng, Caulis Clematidis Armandii, purslane speedwell Herba, Radix Schefflerae Delavayi and Herba Adenostemmatis.
In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 20-35 weight portion, Radix Astragali 20-35 weight portion, Radix Angelicae Sinensis 15-30 weight portion, Radix Codonopsis 15-30 weight portion, Herba Asplenii Incisi 20-35 weight portion, Fructus Liquidambaris 15-25 weight portion, Herba Stellariae Neglectae 10-25 weight portion, Radix Rhapontici 10-25 weight portion, Radix Paeoniae Alba 20-35 weight portion, pungent ginseng 10-25 weight portion, Caulis Clematidis Armandii 10-20 weight portion, purslane speedwell Herba 10-20 weight portion, Radix Schefflerae Delavayi 10-25 weight portion and Herba Adenostemmatis 10-25 weight portion.
In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 23-32 weight portion, Radix Astragali 25-30 weight portion, Radix Angelicae Sinensis 19-25 weight portion, Radix Codonopsis 20-25 weight portion, Herba Asplenii Incisi 24-32 weight portion, Fructus Liquidambaris 17-23 weight portion, Herba Stellariae Neglectae 15-20 weight portion, Radix Rhapontici 15-20 weight portion, Radix Paeoniae Alba 26-31 weight portion, pungent ginseng 14-22 weight portion, Caulis Clematidis Armandii 14-18 weight portion, purslane speedwell Herba 13-17 weight portion, Radix Schefflerae Delavayi 15-20 weight portion and Herba Adenostemmatis 15-20 weight portion.
In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 26 weight portions, the Radix Astragali 27 weight portions, Radix Angelicae Sinensis 21 weight portions, Radix Codonopsis 23 weight portions, Herba Asplenii Incisi 29 weight portions, Fructus Liquidambaris 19 weight portions, Herba Stellariae Neglectae 18 weight portions, Radix Rhapontici 18 weight portions, Radix Paeoniae Alba 29 weight portions, pungent ginseng 18 weight portions, Caulis Clematidis Armandii 16 weight portions, purslane speedwell Herba 15 weight portions, Radix Schefflerae Delavayi 17 weight portions and Herba Adenostemmatis 17 weight portions.
Described Chinese medicine composition also comprises: Ganoderma, meal soup, Fructus Jujubae, Fructus Crataegi and Massa Medicata Fermentata.
In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 20-35 weight portion, Radix Astragali 20-35 weight portion, Radix Angelicae Sinensis 15-30 weight portion, Radix Codonopsis 15-30 weight portion, Herba Asplenii Incisi 20-35 weight portion, Fructus Liquidambaris 15-25 weight portion, Herba Stellariae Neglectae 10-25 weight portion, Radix Rhapontici 10-25 weight portion, Radix Paeoniae Alba 20-35 weight portion, pungent ginseng 10-25 weight portion, Caulis Clematidis Armandii 10-20 weight portion, purslane speedwell Herba 10-20 weight portion, Radix Schefflerae Delavayi 10-25 weight portion, Herba Adenostemmatis 10-25 weight portion, Ganoderma 20-30 weight portion, meal soup 10-20 weight portion, Fructus Jujubae 10-20 weight portion, Fructus Crataegi 10-25 weight portion and Massa Medicata Fermentata 10-20 weight portion.
In order better to realize foregoing invention object, the present invention also provides a kind of preparation method that is used for the treatment of the Chinese medicine composition of bovine subclinical mastitis, the dosage form of described Chinese medicine composition is powder, described preparation method comprises the following steps: according to ratio of weight and number, take all raw medicinal materials, input pulverizer is pulverized, cross 80-120 mesh sieve, obtain powder.
In order better to realize foregoing invention object, the present invention provides again a kind of preparation method that is used for the treatment of the Chinese medicine composition of bovine subclinical mastitis, and the dosage form of described Chinese medicine composition is that described Chinese medicine composition is unguentum, and described preparation method comprises the following steps:
A, by the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis ethanol extraction, concentrated, obtain concentrated solution 1;
B, put into ethanol after other raw medicinal materials are pulverized and soak, extract the concentrated concentrated solution 2 that to obtain;
C, above-mentioned two kinds of concentrated solutions 1 and concentrated solution 2 mixed, mix vaseline or Cera Flava is made unguentum.
In described step a, according to described ratio of weight and number, get the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis, after soak with ethanol 2-3 hour of use 70-80%, 70～85 ℃ of heat are extracted, and ethanol consumption used is 10 times of amounts of the Radix Astragali, Radix Angelicae Sinensis and three kinds of raw medicinal material gross weights of Radix Codonopsis; The concentrated ethanol extract of heating, is concentrated into the concentrated solution 1 that under room temperature, relative density is 1.08, stand for standby use.
In described step b, raw medicinal material described in all the other is mixed by proportioning, drop into pulverizer and be ground into fine powder, after sieving, puts into fine powder acid and alkali-resistance infusion kettle, at normal temperatures and pressures, with common the dipping 10～15 days of ethanol of 70-85%, the liquid having flooded and medicinal residues are carried out to squeeze and filter, after separation, get filtrate, obtain concentrated solution 2.
The beneficial effect that the technical scheme that the embodiment of the present invention provides is brought is: Chinese medicine composition of the present invention has the effect of heat-clearing and toxic substances removing, the gentle middle pain relieving of dissipating blood stasis for subsidence of swelling, oral administration and externally used, in skin penetrates body, strengthens therapeutical effect, improves curative effect; This Chinese medicine composition internal and external application, outside interior supporting, treating both the principal and secondary aspects of a disease, both mutually promote Orally taken and externally medicine, improve autoimmunity, and milch cow is not had side effects, safe and effective.
The specific embodiment
Embodiment 1 powder
The embodiment of the present invention provides a kind of Chinese medicine composition that is used for the treatment of bovine subclinical mastitis, and in Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 26 weight portions, the Radix Astragali 27 weight portions, Radix Angelicae Sinensis 21 weight portions, Radix Codonopsis 23 weight portions, Herba Asplenii Incisi 29 weight portions, Fructus Liquidambaris 19 weight portions, Herba Stellariae Neglectae 18 weight portions, Radix Rhapontici 18 weight portions, Radix Paeoniae Alba 29 weight portions, pungent ginseng 18 weight portions, Caulis Clematidis Armandii 16 weight portions, purslane speedwell Herba 15 weight portions, Radix Schefflerae Delavayi 17 weight portions and Herba Adenostemmatis 17 weight portions.
The dosage form of above-mentioned Chinese medicine composition is powder, and its preparation method comprises the following steps: according to ratio of weight and number, take all raw medicinal materials, drop into pulverizer and pulverize, cross 120 mesh sieves, obtain powder.
Embodiment 2 unguentum
The embodiment of the present invention provides a kind of Chinese medicine composition that is used for the treatment of bovine subclinical mastitis, in Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 27 weight portions, the Radix Astragali 27 weight portions, Radix Angelicae Sinensis 23 weight portions, Radix Codonopsis 22 weight portions, Herba Asplenii Incisi 27 weight portions, Fructus Liquidambaris 20 weight portions, Herba Stellariae Neglectae 17 weight portions, Radix Rhapontici 17 weight portions, Radix Paeoniae Alba 27 weight portions, pungent ginseng 19 weight portions, Caulis Clematidis Armandii 15 weight portions, purslane speedwell Herba 15 weight portions, Radix Schefflerae Delavayi 18 weight portions, Herba Adenostemmatis 17 weight portions, Ganoderma 25 weight portions, meal soup 15 weight portions, Fructus Jujubae 15 weight portions, Fructus Crataegi 17 weight portions and Massa Medicata Fermentata 15 weight portions.
The dosage form of above-mentioned Chinese medicine composition is unguentum, and its preparation method comprises the following steps:
A, by the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis ethanol extraction, concentrated, obtain concentrated solution 1;
B, put into ethanol after other raw medicinal materials are pulverized and soak, extract the concentrated concentrated solution 2 that to obtain;
C, above-mentioned two kinds of concentrated solutions 1 and concentrated solution 2 mixed, mix 85% vaseline or Cera Flava of concentrated solution 1 and concentrated solution 2 mixed weights and make unguentum.
Wherein, in step a, according to described ratio of weight and number, get the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis, the soak with ethanol with 75% is after 3 hours, and 80 ℃ of heat are extracted, and ethanol consumption used is 10 times of amounts of the Radix Astragali, Radix Angelicae Sinensis and three kinds of raw medicinal material gross weights of Radix Codonopsis; The concentrated ethanol extract of heating, is concentrated into the concentrated solution 1 that under room temperature, relative density is 1.08, stand for standby use.
In step b, all the other raw medicinal materials are mixed by proportioning, drop into pulverizer and be ground into fine powder, after fine powder sieves, put into acid and alkali-resistance infusion kettle, at normal temperatures and pressures, jointly flood 12 days with 80% ethanol, the liquid having flooded and medicinal residues are carried out to squeeze and filter, after separation, get filtrate, obtain concentrated solution 2.
Embodiment 3 powders
The embodiment of the present invention provides a kind of Chinese medicine composition that is used for the treatment of bovine subclinical mastitis, in Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 35 weight portions, the Radix Astragali 35 weight portions, Radix Angelicae Sinensis 15 weight portions, Radix Codonopsis 30 weight portions, Herba Asplenii Incisi 20 weight portions, Fructus Liquidambaris 15 weight portions, Herba Stellariae Neglectae 10 weight portions, Radix Rhapontici 25 weight portions, Radix Paeoniae Alba 35 weight portions, pungent ginseng 10 weight portions, Caulis Clematidis Armandii 20 weight portions, purslane speedwell Herba 10 weight portions, Radix Schefflerae Delavayi 25 weight portions, Herba Adenostemmatis 10 weight portions, Ganoderma 25 weight portions, meal soup 10 weight portions, Fructus Jujubae 15 weight portions, Fructus Crataegi 10 weight portions and Massa Medicata Fermentata 20 weight portions.
The dosage form of above-mentioned Chinese medicine composition is powder, and its preparation method comprises the following steps: according to ratio of weight and number, take all raw medicinal materials, drop into pulverizer and pulverize, cross 100 mesh sieves, obtain powder.
Wherein, the pharmacology of each raw medicinal material is as follows:
Semen Vaccariae: hardship, flat.Return liver, stomach warp.Function cures mainly: promoting blood circulation to restore menstrual flow, stimulating milk secretion detumescence.For agalactia, amenorrhea, dysmenorrhea, acute mastitis swells and ache.
The Radix Astragali: sweet, tepor; Return lung, spleen, liver, kidney channel.Admittedly de-for benefiting QI for strengthening the superficies, arresting sweating, to hold in the palm skin ulcer granulation promoting, inducing diuresis to remove edema effect.< < book on Chinese herbal medicine meets former > > to carry: all void of Radix Astragali energy tonifying five ZANG-organs, control stringy pulse spontaneous perspiration, and rush down YIN-fire, remove lung-heat, losslessly send out, there is antiperspirant to stop.Be used for the treatment of the deficiency of vital energy weak, sinking of QI of middle-JIAO, chronic diarrhea proctoptosis, the metrorrhagia of having blood in stool, exterior deficiency spontaneous perspiration, carbuncle is difficult bursts, and burst and do not hold back for a long time, blood deficiency and yellow complexion, interior-heat is quenched one's thirst, chronic nephritis, albuminuria, diabetes etc.The invigorating middle warmer of Radix Astragali Preparata QI invigorating, raw by consolidating superficial resistance holder skin ulcer.The Radix Astragali is the good medicine of promoting resistance and defence disease.
Radix Angelicae Sinensis: sweet in the mouth and weighing, therefore specially can enrich blood, its gas is light and pungent, thus again can promoting the circulation of blood, invigorating middle warmer has, in row, has benefit, really the gas medicine in blood, the also panacea in blood.About mending to mend of assistant, thus can support nutrition blood, QI invigorating spermatogenesis, settling five organs, body by force, beneficial mind, all tangible deficient diseases, nothing is unsuitable.Assistant logical to attack, therefore the pain relieving constipation of dispelling, relieving rigidity of muscles and joints, controls contracture, paralysis, the card such as dry, puckery.Nutrient QI being deficient and exterior syndrome being not relieved person, assistant is with agent such as bavin, Pueraria lobota, fiber crops, osmanthus, the Biao Weire that can fall apart greatly, and show not hold back person, and help with Radix Et Rhizoma Rhei and so on, again can consolidating superficial resistance.Precisely because gas Xin Erdong, thus want its quiet person when keeping away it, the sliding benefaction of property, stool is not consolidated person when keeping away it.All cloudy moderate heat Sheng persons, the active blood of Radix Angelicae Sinensis, also non-institute is suitable, cloudy deficiency of YANG of middle-JIAO person, Radix Angelicae Sinensis can nourish blood, and is indispensable.
Radix Codonopsis: property is flat, sweet in the mouth, micro-acid.Return spleen, lung meridian.Invigorating the spleen and replenishing QI, spleen invigorating lung benefiting.For deficiency of the spleen and lung, the cardiopalmus of breathing hard, anorexia and loose stool, dyspnea due to deficiency cough, interior-heat is quenched one's thirst.The just > > of < < book on Chinese herbal medicine carries: Radix Codonopsis power energy spleen reinforcing nourishing the stomach, and the lung moistening and production of body fluid promoting, gas in tendon fortune, this is very mutually not far away with Radix Ginseng.Its outstanding valuable person, spleen invigorating fortune and not dry, grows stomach-Yin and wet, and lung moistening and do not violate is cold and coolly nourished blood and is not partially grown greasyly, inspires clear sun, gas in vibration, and without just dry fraud.Radix Astragali QI invigorating, can rise enriching spleen-QI, again can lung benefiting consolidating superficial resistance.Radix Codonopsis QI invigorating, can only spleen invigorating QI invigorating, and without the power of consolidating superficial resistance, but Radix Codonopsis can also supplementing QI for promoting the production of body fluid, and the Radix Astragali is without the effect of promoting the production of body fluid, and the Radix Astragali is held concurrently can diuretic, and Radix Codonopsis is without diuretic effect.This two tastes medicine share can invigorating the spleen and replenishing QI, improves resistance.
Herba Asplenii Incisi: bitter, micro-sweet, cool.Function cures mainly: heat-clearing and toxic substances removing, and cough-relieving sore-throat relieving, inducing diuresis and reducing edema, hemostasis and pain-relieving.For influenza, flu, cough, tonsillitis, parotitis, conjunctival congestion and swelling pain, enteritis, dysentery, agalactia; The swollen furunculosis of skin ulcer is controlled in external, traumatic injury, and eczema, burn and scald, knife injury is hemorrhage.
Fructus Liquidambaris: hardship, flat.Return liver, kidney channel.Function cures mainly: dispelling, collateral-activating, diuretic stimulates the menstrual flow.For arthralgia, numbness contracture, edema distension, the few amenorrhea of breast.
Herba Stellariae Neglectae: micro-hardship; Cool in nature.Return stomach; The heart; Liver Channel; Function cures mainly: heat-clearing and toxic substances removing; Treating stranguria; Blood stasis dispelling.Main sick pyogenic infections from tumour or sore; Scall; Mastalgia; Hemorrhoid; Dysentery; Toothache; Pyretic stranguria; Puerperal abdonimal pain.
Radix Rhapontici: hardship, cold.Return stomach warp.Function cures mainly: heat-clearing and toxic substances removing, and eliminating carbuncle, stimulating milk secretion, relaxing muscles and tendons is promoted blood circulation.For acute mastitis, swell and ache, carbuncle carbuncle on the back, scrofula sore, galactostasis, arthralgia chiefly caused by damp pathogen contracture.
Radix Paeoniae Alba: cool in nature, bitter in the mouth acid, is slightly cold, and has the easing the affected liver of enriching blood, suppressing the hyperactive liver pain relieving.Yin fluid astringing is received the effects such as antiperspirant, is applicable to fever due to yin deficiency, menoxenia, the breast abdomen pain over the hypochondriac region, extremity contraction anxious, dysentery stomachache, spontaneous sweating, metrorrhagia, is with inferior disease.That paeoniflorin has is antibacterial, antipyretic, antiinflammatory, increase coronary flow, improve the effect of myocardial nutritional flow, blood vessel dilating, antagonism acute myocardial ischemia, anticoagulant, calmness, analgesia, spasmolytic, antiulcer, adjusting blood glucose.Radix Paeoniae Alba decoct can suppress dysentery bacterium, streptococcus pneumoniae, escherichia coli, Bacillus typhi, Hemolytic streptococcus, bacillus pyocyaneus etc.
Pungent ginseng: sweet in the mouth; Bitter; Cool in nature.Function cures mainly: regulating qi-flowing for strengthening spleen; Removing toxic substances and promoting subsidence of swelling.Main dyspepsia; Abdominal distention; Diarrhoea; Cough with asthma breast is vexed; Flu; Laryngopharynx swelling and pain; Drug intoxication; Traumatic injury congestive edema.
Caulis Clematidis Armandii: light, bitter, cold.GUIXIN, lung, small intestinal, urinary bladder channel.Function cures mainly: clearing away heat and promoting diuresis, the stimulating milk secretion of stimulating the menstrual flow.For edema, gonorrhea, urinary obstruction, arthralgia, amenorrhea breast is few.
Purslane speedwell Herba: sweet, bitter, temperature.Function cures mainly: invigorates blood circulation, and hemostasis, detumescence, pain relieving.For spitting blood, epistaxis, spitting of blood, has blood in stool; Fracture is controlled in external, traumatic injury, and congestion swells and ache.
Ganoderma: property is sweet, flat.GUIXIN, lung, liver, kidney channel.Cure mainly asthenia, cough, asthma, insomnia, dyspepsia, malignant tumor etc.Animal pharmacology shows experiment: Ganoderma has inhibitory action to nervous system, and blood circulation has blood pressure lowering and strengthens the effect of cardiac contractile force, and respiratory system is had to phlegm-dispelling functions, in addition, also has hepatoprotective, improves immunologic function, antibacterially waits effect.
Radix Schefflerae Delavayi: bitter, puckery, be slightly cold.Function cures mainly: reducing swelling and alleviating pain, expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, relieving constipation.Root, stem: toothache, stomachache, stomachache, constipation, traumatic injury, closed fracture.Bark: rheumatic numbness, arthralgia, traumatic injury stasis of blood pain, soreness of waist and knee joint, stomachache.Leaf: dermatitis, eczema, rubella.
Herba Adenostemmatis: acrid in the mouth; Micro-hardship; Cool in nature.Function cures mainly: heat-clearing and toxic substances removing; Expelling wind and removing dampness.Main cold, fever; Icterohepatitis; Cough due to lung-heat; Laryngopharynx swelling and pain; Rheumatism pyretic arthralgia; Acute mastitis; Carbuncle furuncle; Venom.
Meal soup: micro-hardship.Function cures mainly: spleen invigorating.Cure mainly weakness of the spleen and stomach, stomach is indigestion and loss of appetite blunt.
Fructus Jujubae: there is immunosuppressive action, the ethanol extract of Fructus Jujubae with every day 100mg to rat oral gavage, show that Fructus Jujubae and azathioprine have same immunosuppressant; To atopic diseases, can suppress the generation of antibody.Fructus Jujubae ethanol extract may have inhibitory action to rat reactive antibody, and non-reacted antibody is not produced to other effects of inhibitory action: the two glucoside A of flavone in Fructus Jujubae, pharmacological evaluation proof has calmness, hypnosis and hypotensive effect, Fructus Jujubae to have the effect that increases cAMP in leukocyte.Fructus Jujubae decoct can reduce the damage of CCl4 to Rabbit Liver.Can extend the swimming time of mice.Report Fructus Jujubae has the proliferation function of anticancer recently.
Fructus Crataegi: sour in the mouth, sweet, slightly warm in nature, returns spleen, stomach, Liver Channel, and for promoting digestion and invigorating the stomach, circulation of qi promoting dissipating blood stasis, is mainly used to treat accumulation of food in the stomach and intes tine due to indigestion, have loose bowels, dysentery, Fructus Crataegi is the staple for the treatment of dyspepsia, Fructus Crataegi can increase the secretion of peptic digestion enzyme, facilitating digestion; Dysentery bacterium, escherichia coli, bacillus pyocyaneus, staphylococcus aureus etc. are had to obvious inhibitory action.
Massa Medicata Fermentata: for whole wheat flour and other drug (Herba Artemisiae Annuae, Herba Xanthii, the buds such as Herba polygoni hydropiperis, Semen Armeniacae Amarum, Semen Phaseoli) mix the processed goods forming by fermentation.Get Massa Medicata Fermentata and put in pot, to fry to appearance and be coke black, inner coke yellow, take out, spray the water that disappears, cool, be Massa Medicata Fermentata (parched to brown).Massa Medicata Fermentata forms through fermentation, and the product of all fermentations have the digestant effect of strengthening the spleen and stomach, therefore, for bad, the feeling of stuffiness in chest abdominal distention of the digestion due to diet internal injury, quite has effect.
Toxicity test
1, acute toxicity test for oral administration:
40 of application NIH mices, SPF level, male and female half and half, body weight 16～20g, carries out acute toxicity test.Mice is divided into two groups at random, and 20 every group, matched group and administration group, test front fasting 12 hours; The powder of embodiments of the invention 1 preparation is dissolved in water, (concentration is 6.58g crude drug/ml, maximum concentration) gavage, gavage volume is that 5ml/kg(is that single-dose dosage is 32.9g crude drug/kg), matched group gives equivalent normal saline, administration in a day 2 times, delivery time 6 hours, after administration, Continuous Observation is 14 days, and records toxic reaction and the death toll of mice.Experimental result shows: with matched group comparison, after administration, mice has no notable difference, experiment Continuous Observation 14 days, and mice overall health of patients, diet, drinking-water, body weight gain are all normal.Mice oral administration gavage powder LD50>32.9g of the present invention crude drug/kg, every day, maximum dosage-feeding was 65.8g crude drug/kg/ day.Chinese medicine clinical application amount of the present invention is 418g crude drug/day/head, and Niu Tichong is in 700kg, and average dosage is 0.597g crude drug/kg/ day.By weighing machine: the dosis tolerata of mice (average weight take 20g) oral administration gavage Chinese medicine of the present invention is quantity 110 times.Therefore Chinese medicine composition acute toxicity of the present invention is low, clinical drug safety.
2, long term toxicity test for oral administration:
By the powder of the embodiment of the present invention 3 to mice by 11.8,32.9 and 12 weeks (1.0ml/100g body weight of 65.8g crude drug/kg/ day continuous use, every day 2 times) and drug withdrawal after 4 weeks, result shows: Chinese medicine composition of the present invention all has no significant effect indexs such as the hair of rat, behavior, defecation, body weight, organ weights, hemogram, hepatic and renal function, blood glucose, blood fat, internal organs naked eyes do not find that difference changes and histological examination result shows, medication 12 weeks and drug withdrawal are after 4 weeks, and each internal organs of rat are all without obviously changing.Illustrate that toxicity is little after Chinese medicine composition of the present invention is to rat long-term prescription, after drug withdrawal, also there is no difference reaction, application safety.
3, external acute skin irritation test:
Get male 4 of Japan large ear rabbit, body weight 2kg～3kg, get the unguentum of the 0.5g embodiment of the present invention 2, directly spread upon on the skin of animal left side (opposite side in contrast), adopt blocking test, 4 hours application time, after off-test, use the residual tested material of warm water washing, after removing tested material, 1h, 24h, 48h and 72h observe respectively the dermoreaction of the tested district of animal and check plot, and top average is " 0 ", judge that these product are to animal skin nonirritant.
Clinical trial
(1) material is prepared---and test milch cow and place are chosen
Selecting Jinan, Shandong Province city dairy cow farm is Experimental Base, and the milch cow of 700kg of take is experimental subject, starts the milch cow quantity that statistics is suffered from mastitis in June, 2012, to during in May, 2013 totally 369.
(2) diagnostic method: adopt milk pH to measure and Ruzhong somatic cell inspection.Milk pH measures: adopt the blue test of bromine Moschus oxalic acid, and 500 milliliters of the ethanol that main agents is 47%, blue 1.0 grams of bromine Moschus oxalic acid, 5%NaOH1.3 milliliter～1.5 milliliter, stirring and evenly mixing, is green, and pH is 7.0.Get tested newborn 5 milliliters, 1 milliliter of reagent adding, mixes, and observes color and judges.Yellow green (pH6.5 is following) is normal breast, and green (pH6.6) is suspicious, blue positive to dark green (more than pH6.6).Ruzhong somatic cell checks: according to the somatic number in Ruzhong, judge whether milch cow suffers from latent mammitis, and somatic number is negative below 200,000 at every milliliter; Somatic number just likely suffers from latent mammitis every milliliter 200,000～500,000, and somatic number more than 500,000 suffers from latent mammitis or clinic mastitis certainly at every milliliter.
(2) contrast test
Select 360 of ill milch cows to take respectively different treatment meanss to cure as test milch cow, 360 cow heads are divided into four groups at random, 90 every group, are respectively treatment group 1-3 and matched group; Therapeutic Method is respectively: treatment group 1 adopts the powder of embodiment 1 and the unguentum partner treatment of embodiment 2; Treatment group 2 adopts the unguentum of embodiment 2 and the powder partner treatment of embodiment 3; Treatment group 3 adopts the powder partner treatment of embodiment 3; Matched group adopts neck injection penicillin, and injection in two days once, is injected continuously 5-7 days, and gavaged Chinese medicine " GONGYINGSAN ", and every day 1 time, each 250g, takes 1 week continuously.Wherein, the using method of the powder of embodiment 1 and embodiment 2 is for to take with warm water, each 300g, and once a day, every day smeared breast three times by the unguentum of embodiment 2, treats one week.
(3) therapeutic outcome
After treatment finishes, adopt milk pH to measure and detect.Testing result is positive is judged to be recovery from illness.Therapeutic outcome synopsis is as shown in table 1.
Table 1 therapeutic effect synopsis
| Group | Treatment group 1 | Treatment group 2 | Treatment group 3 | Matched group |
| Cure rate | 95.56% | 97.78% | 92.22% | 77.78% |
From the data of table 1, the bovine subclinical mastitis of the treatment for the treatment of group 1-3 is obviously better than matched group 1.After the treatment of a week, carry out following the tracks of half a year and understand, the milch cow of fully recovering in discovery matched group is easily recurred, and the milch cow of the recurrence in matched group produces drug resistance; During treatment, observe to find, treatment group 1-3 cow feeding amount with ill before compare slightly and increase, and matched group feed intake slightly declines.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (10)
1. the Chinese medicine composition that is used for the treatment of bovine subclinical mastitis, it is characterized in that, described Chinese medicine composition comprises following raw medicinal material: Semen Vaccariae, the Radix Astragali, Radix Angelicae Sinensis, Radix Codonopsis, Herba Asplenii Incisi, Fructus Liquidambaris, Herba Stellariae Neglectae, Radix Rhapontici, Radix Paeoniae Alba, pungent ginseng, Caulis Clematidis Armandii, purslane speedwell Herba, Radix Schefflerae Delavayi and Herba Adenostemmatis.
2. the Chinese medicine composition that is used for the treatment of bovine subclinical mastitis according to claim 1, it is characterized in that, in described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 20-35 weight portion, Radix Astragali 20-35 weight portion, Radix Angelicae Sinensis 15-30 weight portion, Radix Codonopsis 15-30 weight portion, Herba Asplenii Incisi 20-35 weight portion, Fructus Liquidambaris 15-25 weight portion, Herba Stellariae Neglectae 10-25 weight portion, Radix Rhapontici 10-25 weight portion, Radix Paeoniae Alba 20-35 weight portion, pungent ginseng 10-25 weight portion, Caulis Clematidis Armandii 10-20 weight portion, purslane speedwell Herba 10-20 weight portion, Radix Schefflerae Delavayi 10-25 weight portion and Herba Adenostemmatis 10-25 weight portion.
3. the Chinese medicine composition that is used for the treatment of bovine subclinical mastitis according to claim 1 and 2, it is characterized in that, in described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 23-32 weight portion, Radix Astragali 25-30 weight portion, Radix Angelicae Sinensis 19-25 weight portion, Radix Codonopsis 20-25 weight portion, Herba Asplenii Incisi 24-32 weight portion, Fructus Liquidambaris 17-23 weight portion, Herba Stellariae Neglectae 15-20 weight portion, Radix Rhapontici 15-20 weight portion, Radix Paeoniae Alba 26-31 weight portion, pungent ginseng 14-22 weight portion, Caulis Clematidis Armandii 14-18 weight portion, purslane speedwell Herba 13-17 weight portion, Radix Schefflerae Delavayi 15-20 weight portion and Herba Adenostemmatis 15-20 weight portion.
4. according to the Chinese medicine composition that is used for the treatment of bovine subclinical mastitis described in claim 1-3 any one, it is characterized in that, in described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 26 weight portions, the Radix Astragali 27 weight portions, Radix Angelicae Sinensis 21 weight portions, Radix Codonopsis 23 weight portions, Herba Asplenii Incisi 29 weight portions, Fructus Liquidambaris 19 weight portions, Herba Stellariae Neglectae 18 weight portions, Radix Rhapontici 18 weight portions, Radix Paeoniae Alba 29 weight portions, pungent ginseng 18 weight portions, Caulis Clematidis Armandii 16 weight portions, purslane speedwell Herba 15 weight portions, Radix Schefflerae Delavayi 17 weight portions and Herba Adenostemmatis 17 weight portions.
5. according to the Chinese medicine composition that is used for the treatment of bovine subclinical mastitis described in claim 1-4 any one, it is characterized in that, described Chinese medicine composition also comprises: Ganoderma, meal soup, Fructus Jujubae, Fructus Crataegi and Massa Medicata Fermentata.
6. according to the Chinese medicine composition that is used for the treatment of bovine subclinical mastitis described in claim 1-5 any one, it is characterized in that, in described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 20-35 weight portion, Radix Astragali 20-35 weight portion, Radix Angelicae Sinensis 15-30 weight portion, Radix Codonopsis 15-30 weight portion, Herba Asplenii Incisi 20-35 weight portion, Fructus Liquidambaris 15-25 weight portion, Herba Stellariae Neglectae 10-25 weight portion, Radix Rhapontici 10-25 weight portion, Radix Paeoniae Alba 20-35 weight portion, pungent ginseng 10-25 weight portion, Caulis Clematidis Armandii 10-20 weight portion, purslane speedwell Herba 10-20 weight portion, Radix Schefflerae Delavayi 10-25 weight portion, Herba Adenostemmatis 10-25 weight portion, Ganoderma 20-30 weight portion, meal soup 10-20 weight portion, Fructus Jujubae 10-20 weight portion, Fructus Crataegi 10-25 weight portion and Massa Medicata Fermentata 10-20 weight portion.
7. the preparation method of the Chinese medicine composition that is used for the treatment of bovine subclinical mastitis as described in claim 1-6 any one, it is characterized in that, the dosage form of described Chinese medicine composition is powder, described preparation method comprises the following steps: according to ratio of weight and number, take all raw medicinal materials, input pulverizer is pulverized, cross 80-120 mesh sieve, obtain powder.
8. a preparation method for the Chinese medicine composition that is used for the treatment of bovine subclinical mastitis as described in claim 1-6 any one, is characterized in that, the dosage form of described Chinese medicine composition is that described Chinese medicine composition is unguentum, and described preparation method comprises the following steps:
A, by the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis ethanol extraction, concentrated, obtain concentrated solution 1;
B, put into ethanol after other raw medicinal materials are pulverized and soak, extract the concentrated concentrated solution 2 that to obtain;
C, above-mentioned two kinds of concentrated solutions 1 and concentrated solution 2 mixed, mix vaseline or Cera Flava is made unguentum.
9. the preparation method that is used for the treatment of the Chinese medicine composition of bovine subclinical mastitis according to claim 8, it is characterized in that, in described step a, according to described ratio of weight and number, get the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis, with behind soak with ethanol 2-3 hour of 70-80%, 70～85 ℃ of heat are extracted, and ethanol consumption used is 10 times of amounts of the Radix Astragali, Radix Angelicae Sinensis and three kinds of raw medicinal material gross weights of Radix Codonopsis; The concentrated ethanol extract of heating, is concentrated into the concentrated solution 1 that under room temperature, relative density is 1.08, stand for standby use.
10. the preparation method of the Chinese medicine composition that is used for the treatment of bovine subclinical mastitis according to claim 8 or claim 9, it is characterized in that, in described step b, raw medicinal material described in all the other is mixed by proportioning, drop into pulverizer and be ground into fine powder, after sieving, puts into fine powder acid and alkali-resistance infusion kettle, at normal temperatures and pressures, with common the dipping 10～15 days of ethanol of 70-85%, the liquid having flooded and medicinal residues are carried out to squeeze and filter, after separation, get filtrate, obtain concentrated solution 2.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304809A (en) * | 2014-11-09 | 2015-01-28 | 蒋法成 | Feed additive for cow during lactation period and application of feed additive |
| RU2582269C1 (en) * | 2015-03-04 | 2016-04-20 | Анатолий Николаевич Иванников | Method of treating mastitis in cattle |
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| CN101011491A (en) * | 2007-01-24 | 2007-08-08 | 辽宁好护士药业（集团）有限责任公司 | Granule for expelling obstruction of galactophore and its preparation method |
| CN102151325A (en) * | 2011-04-13 | 2011-08-17 | 苑学 | Traditional Chinese medicine for treating breast diseases |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011491A (en) * | 2007-01-24 | 2007-08-08 | 辽宁好护士药业（集团）有限责任公司 | Granule for expelling obstruction of galactophore and its preparation method |
| CN102151325A (en) * | 2011-04-13 | 2011-08-17 | 苑学 | Traditional Chinese medicine for treating breast diseases |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304809A (en) * | 2014-11-09 | 2015-01-28 | 蒋法成 | Feed additive for cow during lactation period and application of feed additive |
| RU2582269C1 (en) * | 2015-03-04 | 2016-04-20 | Анатолий Николаевич Иванников | Method of treating mastitis in cattle |
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