Background technology:
In recent years, because make friends with on shortage, fish meal price one tunnel of fish meal resource, Feed Enterprise has to adopt the consumption that improves vegetable protein in feed to come the consumption of Substitute For Partial or whole fish meal to reduce feed cost, but so just caused the appearance of new problem, because thereby a large amount of uses of vegetable protein have affected local flavor, the palatability of feed, in addition, because vegetable protein exists ANFs, digestibility low, cause the speed of growth of aquatic livestock and intestines and stomach health to be adversely affected.
The aquatic feeds phagostimulant of finding at present has amino acid, alkaloid, TMAO(TMAO), DMPT(dimethyl-DMPT) and nucleotides etc., TMAO wherein, the food calling effect of DMPT is relatively good, these two kinds of materials can be by stimulating the olfactory organ of aquatic livestock, thereby promote to ingest, and nucleotides, amino acid, alkaloid promotes ingesting of aquatic livestock by improving the taste perception of feed, but facts have proved, single or simply above-mentioned substance is carried out to interworking and can only play at short notice food calling effect, long-time result of use is also not obvious.Although its reason is main or add after the above-mentioned phagostimulant with food calling effect, although food ration has increased, but can not improve digesting and assimilating of feed, and in feed vegetable protein itself digest and assimilate low, excess intake can cause indigestion on the contrary, affect the health of digestive system, thereby affect the growth of aquatic livestock.Therefore, how to develop a kind of food calling effect that can improve aquatic feeds and can improve again the focus that aquatic feeds phagostimulant that aquatic feeds digests and assimilates has become research.
Summary of the invention:
The object of this invention is to provide a kind of quality stability, security environmental protection and aquatic feeds phagostimulant easy to process.
Aquatic feeds phagostimulant of the present invention, is characterized in that, by gross mass mark 100%, comprise yeast extract 40~50%, DMPT(dimethyl-DMPT) 5~10%, TMAO(TMAO) 20~30%, bile salt 5~10%, filler 10~20%.
Described yeast extract, preferably preparation by the following method:
Every 10L quality beer saccharomycete, after removal of impurities after filtration,, debitterize are processed, centrifugal in supercentrifuge, rotating speed is 4000r/min, centrifugal 30min adds papain 50000IU, trypsase 100000IU, insulation reaction in reative cell after centrifugal in thalline, temperature is controlled at 45 ℃, reacts 24 hours; Improve subsequently temperature to 95 ℃, insulation reaction is 2.5 hours again, add 2 kilograms, rice bran, then spraying is dry, and spray-dired shower nozzle rotating speed is 15000r/min, and inlet temperature is 250 ℃, outlet temperature is 110 ℃, negative pressure 90mpa, obtains yeast extract, by the cooling rear packing of the yeast extract of gained.
Described filler, preferably rice bran meal.
Aquatic feeds phagostimulant of the present invention is prepared by the following method, and all raw materials, according to its mass percent mixing and stirring, are obtained to aquatic feeds phagostimulant of the present invention.
The present invention carries out multiple single phagostimulant and the bile salt with short digestion, hepatic cholagogic function composite, and aquatic livestock is had to good food calling effect and short digestion effect.Aquatic feeds phagostimulant of the present invention is added in aquatic feeds, and its bile salt containing can effectively promote digesting and assimilating of feed.Aquatic feeds phagostimulant of the present invention is safe, containing poisonous and harmful substance, addition is low, selected material quality is stablized controlled, product is widely applicable.
The specific embodiment:
Following examples are to further illustrate of the present invention, rather than limitation of the present invention.
The test method of using in following examples if no special instructions, is conventional method.
The material using in following examples, reagent, if no special instructions, obtain in all can be from commercial channels.
Embodiment 1:
The preparation of yeast extract:
Every 10L quality beer saccharomycete, after removal of impurities after filtration,, debitterize are processed, centrifugal in supercentrifuge, rotating speed is 4000r/min, centrifugal 30min adds papain 50000IU, trypsase 100000IU, insulation reaction in reative cell after centrifugal in thalline, temperature is controlled at 45 ℃, reacts 24 hours; Improve subsequently temperature to 95 ℃, insulation reaction is 2.5 hours again, add 2 kilograms, rice bran, then spraying is dry, and spray-dired shower nozzle rotating speed is 15000r/min, and inlet temperature is 250 ℃, outlet temperature is 110 ℃, negative pressure 90mpa, obtains yeast extract, by the cooling rear packing of the yeast extract of gained.
By gross mass mark 100%, yeast extract 40%, DMPT(dimethyl-DMPT) 5%, TMAO(TMAO) 30%, bile salt 5%, rice bran meal 20%, by these composition mixing and stirring, obtains aquatic feeds phagostimulant of the present invention.
Embodiment 2:
The preparation of yeast extract:
Every 10L quality beer saccharomycete, after removal of impurities after filtration,, debitterize are processed, centrifugal in supercentrifuge, rotating speed is 4000r/min, centrifugal 30min adds papain 50000IU, trypsase 100000IU, insulation reaction in reative cell after centrifugal in thalline, temperature is controlled at 45 ℃, reacts 24 hours; Improve subsequently temperature to 95 ℃, insulation reaction is 2.5 hours again, add 2 kilograms, rice bran, then spraying is dry, and spray-dired shower nozzle rotating speed is 15000r/min, and inlet temperature is 250 ℃, outlet temperature is 110 ℃, negative pressure 90mpa, obtains yeast extract, by the cooling rear packing of the yeast extract of gained.
By gross mass mark 100%, yeast extract 50%, DMPT(dimethyl-DMPT) 10%, TMAO(TMAO) 20%, bile salt 10%, rice bran meal 10%, by these composition mixing and stirring, obtains aquatic feeds phagostimulant of the present invention.
By 4 weeks ingest and growth test is assessed phagostimulant, concrete scheme is: the raw fish commercial feed of take adds respectively 2% cuttlefish cream (W), the aquatic feeds phagostimulant (A) of 0.2% embodiment 1 preparation and aquatic feeds (B) prepared by 0.2% the present embodiment as control group (D), in raw fish commercial feed, make 4 groups of test feeds, raise the raw fish 4 weeks that initial body weight is about 50g/ tail.
Result is as shown in table 1; Food ration, rate of body weight gain, the feed coefficient of the raw fish of DIFFERENT FEED of showing to feed is followed successively by: grazing rate, rate of body weight gain: B>A>W>D, feed coefficient: D>W>A>BGreatT. GreaT.GT, wherein rate of body weight gain and the food ration of B group are significantly higher than other each groups, (P < 0.05), feed coefficient B organizes significantly lower than D group.
In table 1. feed, add the impact that phagostimulant and cuttlefish cream are ingested and grown raw fish
By 4 weeks ingest and growth test is assessed phagostimulant, concrete scheme is: take low fish meal penaeus vannamei boone feed as control group (D), in control group feed, add respectively 0.2% TMAO(T), 0.03%DMPT(DM) and the aquatic feeds phagostimulant (B) prepared of the present embodiment of 0.2%, make 4 groups of test feeds, raise initial body weight and be the Penaeus Vannmei 4 weeks of 2.3g/ tail.
Result is as shown in table 2: food ration, the rate of body weight gain of the DIFFERENT FEED of feeding Penaeus Vannmei are followed successively by: B>DM>T>D, and wherein rate of body weight gain, grazing rate and the protein efficiency ratio, PER of B group are significantly higher than other each groups (P < 0.05); The feed coefficient of B group is significantly lower than other each groups; Rate of body weight gain, protein efficiency ratio, PER and the food ration of TMAO and DMPT group are all significantly higher than control group (P < 0.05), the rate of body weight gain of DMPT group is significantly higher than TMAO group (P < 0.05), but feed coefficient, food ration and the protein efficiency ratio, PER of TMAO group and DMPT group do not have significant difference.
In table 2. feed, add that different phagostimulants are ingested on Penaeus Vannmei and the impact of growth performance