CN105230985A - Aquafeed feeding-promoting agent with health-care function and preparation method thereof - Google Patents
Aquafeed feeding-promoting agent with health-care function and preparation method thereof Download PDFInfo
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- CN105230985A CN105230985A CN201510708854.XA CN201510708854A CN105230985A CN 105230985 A CN105230985 A CN 105230985A CN 201510708854 A CN201510708854 A CN 201510708854A CN 105230985 A CN105230985 A CN 105230985A
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Abstract
The invention provides an aquafeed feeding-promoting agent with a health-care function and a preparation method thereof. The feeding-promoting agent is composed of a yeast extract product and an auxiliary agent. A preparation method of the yeast extract product comprises the following steps: 1) weighing yeast milk or yeast powder as raw material, heating the material to 50-65 DEG C, and simultaneously inactivating an enzyme system of a yeast under the pressure of 50-70 MPa; 2) cooling the yeast milk or the yeast powder after the enzyme inactivation in the step 2) to 50-55 DEG C, adjusting the pH value to 5.0-7.0, adding a compound enzyme system, and carrying out an enzymatic hydrolysis reaction for 18-24 hours; 3) heating an enzymatic hydrolysate obtained in the step 2) to 65-70 DEG C, adjusting the pH value to 5.0-6.0, adding nuclease, carrying out an enzymatic hydrolysis reaction for 5-10 hours; and 4) heating the enzymatic hydrolysate obtained in the step 3) to 75-90 DEG C, carrying out inactivation for 0.5-1 hours, after the inactivation, cooling the temperature to 60-70 DEG C, and obtaining a yeast extract. The feeding-promoting agent has a good feeding-promoting effect for aquatic animals, and at the same time improves disease-resistant and anti-stress capabilities of aquatic animals.
Description
Technical field
The invention belongs to field of feed additive technology, be specifically related to a kind of aquatic feeds phagostimulant with health care and preparation method thereof.
Background technology
Phagostimulant is also known as attractant, Appetizer or palatability additive, belong to non-nutritional feed additive, have and improve feed palatability, strengthen animal appetite, improve animal feed intake, Feeding Velocity, promote aquatic livestock digesting and assimilating and growing feed, the effect alleviating water pollution He reduce costs.Though phagostimulant is non-nutritive additive, but appropriate interpolation can improve the palatability of feed in aquatic feeds, contribute to aquatic livestock and still can maintain high food ration under stress situation or disease condition, ensure that aquatic livestock has enough nutrition intake, thus raising Abwehrkraft des Koepers, reduce ill probability or alleviate some disease, for healthy aquaculture provides safeguard.In addition, in the face of the situation of nowadays fish meal shortage, the exploitation of phagostimulant can improve the local flavor of low fish meal or fish meal-less feed, alleviate animal protein source problem in short supply, for the exploitation of novel fodder resource provide new approaches.
The aquatic feeds phagostimulant of current discovery has amino acid, alkaloid, TMAO (TMAO), DMPT (Dimethyl-propionic acid thetin) etc.But facts have proved, single or simply carry out interworking to above-mentioned substance and can only play phagostimulating effect at short notice, long-time result of use is also not obvious.Although current CN103734530A has produce aquatic attractant by adding yeast extract, current phagostimulant product function is comparatively single, does not have the health-care effect of develop immunitypty.And in the large scale and high density breeding production of modern aquatic livestock, due to the impact of various factors, the disease-resistant of fishes and shrimps or anti-stress ability is caused to decline, Long term Animal is in sub-health state, disease is multiple, survival rate and economic benefit decline, and have higher requirement to health care food calling feed or health care food calling feed addictive etc. in market.
Summary of the invention
For the phagostimulating effect solving the existence of existing aquatic feeds phagostimulant is undesirable, not there is enhancing immunologic function, the present invention proposes a kind of aquatic feeds phagostimulant with health care, this phagostimulant is by improving the preparation technology of yeast extract, by self enzymatic activity in pressure removing yeast milk or dusty yeast, and retain the activity of himself other albumen, the yeast extract that obtains is hydrolyzed again and auxiliary agent acts synergistically by complex enzyme system, not only be there is good phagostimulating effect, and aquatic livestock non-specific immune systems can be activated, thus improve the disease-resistant and anti-stress ability of aquatic livestock.
Technical scheme of the present invention is achieved in that
Have an aquatic feeds phagostimulant for health care, it is characterized in that, be made up of yeast extract and auxiliary agent, described yeast extract is prepared by the following method and obtains:
1) yeast milk or dusty yeast is taken as raw material, wherein said dusty yeast needs use water to be mixed with quality and volume ratio percent concentrations is 10-15% yeast soln, then be heated to 50-65 DEG C, under 50-70MPa pressure condition, remove the activity of yeast self enzyme system simultaneously;
2) by step 1) go out enzyme live process after yeast milk or yeast soln be cooled to 50-55 DEG C, and regulate pH to 5.0-7.0, then one or more enzymes in papain, alkali protease, neutral proteinase, acid protease are added, enzyme digestion reaction 18-24h;
3) by step 2) enzymolysis liquid that obtains is warming up to 65-70 DEG C, and regulates pH to 5.0-6.0, then adds nuclease, enzyme digestion reaction 5-10h;
4) by step 3) enzymolysis liquid that obtains is warming up to 75-90 DEG C, deactivation 0.5-1h, 60-70 DEG C is cooled to after deactivation, then being separated or liquid after not being separated, is that 30-65% is cream yeast extract or carries out spraying dry and is dusty yeast extract through being evaporated to percent mass Particle density.
Further, described step 1) go out enzyme live process after yeast milk or yeast soln adopt colloid mill carry out homogeneous process.
Further, calculate by weight percentage, described step 2) in the addition of enzyme be the 0.1-0.3% of described yeast milk or dusty yeast, described step 3) in the addition of nuclease be the 0.3-0.6% of described yeast milk or dusty yeast.
Further, calculate according to percetage by weight, the content of described yeast extract is 92-97%, and the content of described auxiliary agent is 3-8%.
Further, described auxiliary agent comprises tasty agents, filler, arginine, glycine, alanine, glutamic acid and lysine.
Further, described tasty agents is 5 '-flavour nucleotide disodium and disodium succinate, and described filler is cyclodextrine.
Further, the content of described yeast extract is 93.5-96.75%, and the content of described 5 '-flavour nucleotide disodium and disodium succinate is 0.3-0.5%, and the content of described cyclodextrine is 2-4%, and described arginic content is 1-2%.
Another object of the present invention is to provide a kind of preparation method with the aquatic feeds phagostimulant of health care, by above-mentioned yeast extract and auxiliary agent mixing and stirring, namely obtains described aquatic feeds phagostimulant.
Further, also comprise ultrafiltration membrane and filter and vacuum-concentrcted step, the aquatic feeds phagostimulant be mixed evenly is filtered by ultrafiltration membrane, then with vacuum-concentrcted to required product concentration.Filtered by ultrafiltration membrane and can remove some water-soluble poor impurity, then meet spray-dired requirement by vacuum decompression to required product concentration.
The aquatic feeds phagostimulant with health care of the present invention is by improving the preparation technology of yeast extract, by self enzymatic activity in pressure removing yeast milk or active dry yeast, and retain the activity of himself other albumen, the yeast extract that obtains is hydrolyzed again and auxiliary agent acts synergistically by complex enzyme system, not only be there is good phagostimulating effect, and aquatic livestock non-specific immune systems can be activated, thus improve the disease-resistant and anti-stress ability of aquatic livestock.
Detailed description of the invention
Embodiment 1
The preparation of yeast extract:
Be raw material with yeast milk, adding water and being settled to mass concentration is 10%.Then be heated to 90-95 DEG C of insulation 30-60 minute, the activity of removing yeast self enzyme system, is cooled to 50-65 DEG C, broken yeast cell under 50-70MPa pressure condition subsequently
Homogeneous 3 times again.Then, regulate pH to 5.8 be 50 DEG C in temperature, add compound protease, enzyme digestion reaction 24h, alkali protease addition is 0.2% of yeast milk weight.Reactant liquor after enzymolysis is regulated pH to 5.0, and be warming up to 65 DEG C, add nuclease (addition of nuclease is 0.3% of yeast milk weight) and carry out enzyme digestion reaction, enzymolysis time is 10h, during enzymolysis, carry out intermittent stirring, stirs 10 minutes every 1h.Be warming up to 75 DEG C of deactivation 1h after enzyme digestion reaction terminates, be then cooled to 60 DEG C, carry out separation with centrifuge and obtain supernatant, be i.e. yeast extract.
There is the preparation of the aquatic feeds phagostimulant of health care:
Get 5 '-flavour nucleotide disodium of supernatant 10kg, 0.03kg, 0.2kg cyclodextrine mixes with 0.1kg ispol (mass ratio of arginine, glycine, alanine, glutamic acid, lysine is 20:5:5:60:10), then 55 DEG C are warming up to, molecular cut off is adopted to be the ultrafiltration membrance filter of 10000da, obtain dislysate, it is 60% that permeate adopts vacuum decompression solute in dislysate to be concentrated into mass concentration.
Embodiment 2
The preparation of yeast extract:
Be raw material with dusty yeast, adding deionized water, to be settled to mass concentration be 15%.Then be heated to 90-95 DEG C of insulation 30-60 minute, the activity of removing yeast self enzyme system, is cooled to 50-65 DEG C, broken yeast cell under 50-70MPa pressure condition subsequently
Homogeneous 3 times again.Then, regulate pH to 5.5 be 50 DEG C in temperature, add compound protease, enzyme digestion reaction 18h, mixture (mass ratio of papain and neutral proteinase the is 5:5) addition of papain and neutral proteinase is 0.3% of yeast milk weight.Reactant liquor after enzymolysis is regulated pH to 6.0, and be warming up to 65 DEG C, add nuclease (addition of nuclease is 0.6% of yeast milk weight) and carry out enzyme digestion reaction, enzymolysis time is 5h, during enzymolysis, carry out intermittent stirring, stirs 10 minutes every 1h.Be warming up to 90 DEG C of deactivation 0.5h after enzyme digestion reaction terminates, be then cooled to 65 DEG C, i.e. yeast extract.
There is the preparation of the aquatic feeds phagostimulant of health care:
Get 5 '-flavour nucleotide disodium and the disodium succinate mixture of above-mentioned solution 10kg, 0.05kg, 0.4kg cyclodextrine mixes with 0.2kg ispol (mass ratio of arginine, glycine, alanine, glutamic acid, lysine is 10:5:15:55:5), then carries out spraying dry and obtains powder product.
Embodiment 3
Be raw material with dusty yeast, adding deionized water, to be settled to mass concentration be 12%.Then be heated to 90-95 DEG C of insulation 30-60 minute, the activity of removing yeast self enzyme system, is cooled to 50-65 DEG C, broken yeast cell under 50-70MPa pressure condition subsequently
Homogeneous 3 times again.Then, regulate pH to 5.8 be 55 DEG C in temperature, add compound protease, enzyme digestion reaction 20h, neutral proteinase addition is 0.15% of yeast milk weight.Reactant liquor after enzymolysis is regulated pH to 5.5, and be warming up to 70 DEG C, add nuclease (addition of nuclease is 0.4% of yeast milk weight) and carry out enzyme digestion reaction, enzymolysis time is 5h, during enzymolysis, carry out intermittent stirring, stirs 10 minutes every 1h.Be warming up to 90 DEG C of deactivation 0.5h after enzyme digestion reaction terminates, be then cooled to 65 DEG C, carry out separation with centrifuge and obtain supernatant, be i.e. yeast extract.
There is the preparation of the aquatic feeds phagostimulant of health care:
Get 5 '-flavour nucleotide disodium of above-mentioned solution 10kg, 0.04kg, disodium succinate mixture, 0.3kg cyclodextrine mixes with 0.15kg ispol (mass ratio of arginine, glycine, alanine, glutamic acid, lysine is 10:5:15:35:35), then carries out spraying dry and obtains powder product.
The aquatic feeds phagostimulant prepared using embodiment 1 is as experimental group, DMPT is as main flow phagostimulant and do not add any phagostimulant as blank group, the method of " the adding different phagostimulant in feed to Penaeus Vannmei growth and the impact of feed digestion " of delivering in " fresh water fishery " the 33rd volume the 5th phase in 2003 according to Liu Lihe, Zheng Shixuan, Zheng Xianchang etc. is to study phagostimulant of the present invention to the impact of Penaeus Vannmei on its immunity, digestion and intestinal microflora, and concrete outcome is as follows:
The different phagostimulant of table 1 is on the impact of Penaeus Vannmei immune indexes
In table 1, SOD is superoxide dismutase, and MDA is MDA.As can be seen from Table 1, add its lysozyme activity of experimental group of embodiment 1, phenoloxidase activity and SOD active all higher than blank group, and all higher than phagostimulant DMPT.The activity of these 3 enzymes is all relevant with biological stress reaction, it can thus be appreciated that embodiment 1 can significantly improve the anti-stress ability of Penaeus Vannmei, namely improves its immunocompetence.
The different phagostimulant of table 2 affects Penaeus Vannmei enteric microorganism
| Heterotrophicy bacteria quantity | Amount of vibrio | Bacillus quantity | Lactic acid bacterium number | |
| Blank group | 2.4*10 10 | 3.9*10 6 | 0 | 0 |
| DMPT | 2.1*10 10 | 3.2*10 6 | 0 | 0 |
| Embodiment 1 | 2.3*10 10 | 2.2*10 5 | 3.2*10 6 | 1.8*10 7 |
As can be seen from Table 2, feeding crosses heterotrophicy bacteria quantity, amount of vibrio, bacillus quantity and lactic acid bacterium number in its enteron aisle of Penaeus Vannmei containing embodiment 1 phagostimulant all higher than blank group, and all higher than phagostimulant DMPT.Thus, the phagostimulant of embodiment 1 can be conducive to the existence of gut flora, there is good health care.
The different phagostimulant of table 3 affects Penaeus Vannmei digestive ferment
As can be seen from Table 3, its digestive ferment of Penaeus Vannmei secretion that feeding is crossed containing embodiment 1 phagostimulant significantly increases, and crosses the Penaeus Vannmei of DMPT higher than feeding.As can be seen here, embodiment 1 has good phagostimulating effect.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. have an aquatic feeds phagostimulant for health care, it is characterized in that, be made up of yeast extract and auxiliary agent, described yeast extract is prepared by the following method and obtains:
1) yeast milk or dusty yeast is taken as raw material, wherein said dusty yeast needs use water to be mixed with quality and volume ratio percent concentrations is 10-15% yeast soln, then be heated to 50-65 DEG C, under 50-70MPa pressure condition, remove the activity of yeast self enzyme system simultaneously;
2) by step 1) go out enzyme live process after yeast milk or yeast soln be cooled to 50-55 DEG C, and regulate pH to 5.0-7.0, then one or more enzymes in papain, alkali protease, neutral proteinase, acid protease are added, enzyme digestion reaction 18-24h;
3) by step 2) enzymolysis liquid that obtains is warming up to 65-70 DEG C, and regulates pH to 5.0-6.0, then adds nuclease, enzyme digestion reaction 5-10h;
4) by step 3) enzymolysis liquid that obtains is warming up to 75-90 DEG C, deactivation 0.5-1h, 60-70 DEG C is cooled to after deactivation, then being separated or liquid after not being separated, is that 30-65% is cream yeast extract or carries out spraying dry and is dusty yeast extract through being evaporated to percent mass Particle density.
2. the aquatic feeds phagostimulant with health care according to claim 1, is characterized in that, described step 1) go out enzyme live process after yeast milk or yeast soln adopt colloid mill carry out homogeneous process.
3. the aquatic feeds phagostimulant with health care according to claim 1 and 2, it is characterized in that, calculate by weight percentage, described step 2) in the addition of enzyme be the 0.1-0.3% of described yeast milk or dusty yeast, described step 3) in the addition of nuclease be the 0.3-0.6% of described yeast milk or dusty yeast.
4. the aquatic feeds phagostimulant with health care according to claim 1 and 2, is characterized in that, calculate according to percetage by weight, the content of described yeast extract is 92-97%, and the content of described auxiliary agent is 3-8%.
5. the aquatic feeds phagostimulant with health care according to claim 4, it is characterized in that, described auxiliary agent comprises tasty agents, filler, arginine, glycine, alanine, glutamic acid and lysine.
6. the aquatic feeds phagostimulant with health care according to claim 5, is characterized in that, described tasty agents is 5 '-flavour nucleotide disodium and disodium succinate, and described filler is cyclodextrine.
7. the aquatic feeds phagostimulant with health care according to claim 6, it is characterized in that, the content of described yeast extract is 93.5-96.75%, the content of described 5 '-flavour nucleotide disodium and disodium succinate is 0.3-0.5%, the content of described cyclodextrine is 2-4%, and described arginic content is 1-2%.
8. there is a preparation method for the aquatic feeds phagostimulant of health care, it is characterized in that, by yeast extract according to claim 1 and auxiliary agent mixing and stirring, namely obtain described aquatic feeds phagostimulant.
9. the preparation method with the aquatic feeds phagostimulant of health care according to claim 8, it is characterized in that, also comprise ultrafiltration membrane to filter and vacuum-concentrcted step, the aquatic feeds phagostimulant be mixed evenly is filtered by ultrafiltration membrane, then with vacuum-concentrcted to required product concentration.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108432977A (en) * | 2018-03-26 | 2018-08-24 | 佛山市顺德区活宝源生物科技有限公司 | A kind of preparation and preparation method thereof promoting snakehead growth based on yeast class component |
| CN108740358A (en) * | 2018-05-17 | 2018-11-06 | 南京同凯兆业生物技术有限责任公司 | A kind of feed addictive and the preparation method and application thereof |
| CN112080540A (en) * | 2020-09-09 | 2020-12-15 | 广西普乐益生物科技有限公司 | Yeast hydrolysate with high nucleotide content and preparation method thereof |
| CN113040295A (en) * | 2021-04-26 | 2021-06-29 | 浙江工业大学 | Artificial compound swimming crab bait and preparation method thereof |
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| CN101053373A (en) * | 2007-05-08 | 2007-10-17 | 朱旺明 | Food attractant growth promoting agent for aquatic animals and its manufacturing technology |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108432977A (en) * | 2018-03-26 | 2018-08-24 | 佛山市顺德区活宝源生物科技有限公司 | A kind of preparation and preparation method thereof promoting snakehead growth based on yeast class component |
| CN108740358A (en) * | 2018-05-17 | 2018-11-06 | 南京同凯兆业生物技术有限责任公司 | A kind of feed addictive and the preparation method and application thereof |
| CN108740358B (en) * | 2018-05-17 | 2021-05-25 | 南京同凯兆业生物技术有限责任公司 | Feed additive and preparation method and application thereof |
| CN112080540A (en) * | 2020-09-09 | 2020-12-15 | 广西普乐益生物科技有限公司 | Yeast hydrolysate with high nucleotide content and preparation method thereof |
| CN113040295A (en) * | 2021-04-26 | 2021-06-29 | 浙江工业大学 | Artificial compound swimming crab bait and preparation method thereof |
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