CN107348126A - Cherish ovum phase loach feed - Google Patents
Cherish ovum phase loach feed Download PDFInfo
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- CN107348126A CN107348126A CN201710640256.2A CN201710640256A CN107348126A CN 107348126 A CN107348126 A CN 107348126A CN 201710640256 A CN201710640256 A CN 201710640256A CN 107348126 A CN107348126 A CN 107348126A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Marine Sciences & Fisheries (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses bosom ovum phase loach feed, the feed ingredient and its parts by weight are:12 16 parts of white fish meal, 20 24 parts of fermented bean dregs, 35 parts of fish oil, 35 parts of soybean oil, 40 45 parts of Strong flour, 0.1 0.3 parts of Choline Chloride, 0.8 1.2 parts of calcium dihydrogen phosphate, 0.1 0.2 parts of bile salt.Have the beneficial effect that:Ovum phase loach feed is cherished in the present invention using fermented bean dregs as protein sources, with bafillus natto to bean pulp fermentation processing, various antigenic components, ANFs in dregs of beans is set to be can be effectively reduced removal, protein in dregs of beans is also broken down into substantial amounts of small peptide, this nonantigenic small peptide is more easy to be absorbed by loach, can significantly improve the fish brood amount of loach, while reduces residual of the feed in water body, reduce the ammonia-nitrogen content in water body, meet green requirement.
Description
Technical field
The present invention relates to aquatic feeds field, more particularly, to bosom ovum phase loach feed.
Background technology
Loach is subordinate to Cypriniformes Cobitidae loach genus, is that one kind blazons small-sized bottom and dwelt ominivore-fish.Due to special
Intestinal respiration function, loach lower oxygen concentration resistance easily transport, strong environmental adaptability, can almost be cultivated in various waters.Loach
Market demand is more stable, and demand for exports amount is larger, and the market price is high, high financial profit.But recently as chemical fertilizer, agriculture
The pollutions such as medicine, industrial wastewater aggravate, and the field ecological environment of loach further deteriorates, and plus the overfishing of the mankind, causes open country
Raw loach resource falls sharply.The yield of wild loach far can not meet the market demand, and development intensive culture is to meet people
The effective way of class demand, and feed is the basis for developing intensive culture.
Prior art such as Authorization Notice No. is the B of CN 104171570 Chinese invention patent, discloses a kind of loach and coordinates
Feed, nutrition is balanced can to make loach be readily adapted to accommodate breeding environment comprehensively, but the feed is non-digestible, and easily in water body
Middle residual, in turn result in the pollution-free of environment.
The content of the invention
It is an object of the invention to provide bosom ovum phase loach feed, feed absorption easy to digest, loach can be significantly improved
Fish brood amount, it is and small to water pollution.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is:Cherish ovum phase loach feed, the feed
Composition and its parts by weight are:White fish meal 12-16 parts, fermented bean dregs 20-24 parts, fish oil 3-5 parts, soybean oil 3-5 parts, Strong flour
40-45 parts, Choline Chloride 0.1-0.3 parts, calcium dihydrogen phosphate 0.8-1.2 parts, bile salt 0.1-0.2 parts.The raw material used
Cost is cheap, easily stored, and the ratio between composition is moderate, and prepared feed palatability is good, is easily digested absorption, Neng Gouxian
The fish brood amount of loach is write, and is remained less in water body, it is small to water pollution.
Preferably, the preparation process of fermented bean dregs includes in feed:Activation culture, bean pulp solid-state fermentation.Dregs of beans is entered
The processing of row biofermentation, it is the small peptide for being more easy to absorb by the larger protein degradation of relative molecular mass contained in dregs of beans,
The digestibility of dregs of beans and the absorptivity of animal are improved, and effectively reduces the content of ANFs in dregs of beans.
Preferably, activation culture process is:Bafillus natto is inoculated in inclined-plane in a manner of sterile working first
Culture medium, 30-33 DEG C of culture 2-3d, the composition and its parts by weight of slant medium are:Beef extract 0.3-0.5 parts, peptone 1-
1.3 parts, sodium chloride 0.5-0.7 parts, agar 2-3 parts, water 90-95 parts;Then liquid is accessed from slant medium picking 1-2 rings bacterium
In activation medium, 34-37 DEG C, 170-180r/min shaken cultivation 6-7h, the composition and its parts by weight of liquid activation medium
For:Beef extract 1.5-2.5 parts, peptone 5-7 parts, sodium chloride 2.5-3.5 parts, water 400-450 parts.Bafillus natto used
Buy in Chinese agriculture Culture Collection.Under the conditions of being somebody's turn to do, the growth vigor of strain becomes strong, and physiological character is more steady
It is fixed, purebred, the shortening process cycle of certain amount and quality can be quickly obtained, and be follow-up bean pulp solid-state fermentation process
Sufficient amount of seed is provided.
Preferably, bean pulp solid-state fermentation process is:By the bafillus natto bacterium solution obtained by activation culture process with
10% inoculum concentration accesses solid medium, adds 0.2-0.4 part active peptides, 35-37 DEG C of culture 60-70h, obtains fermentation beans
The dregs of rice.The preparation method of solid medium is:With plant pulverizer grind dregs of beans, take 7-9 parts dregs of beans, 0.5-1.3 parts wheat bran and
2-3 parts water mixes, and stands 2h, gained mixture is placed in blake bottle, 121 DEG C of sterilizing 25min, obtains solid medium.This
Under part, solid medium utilization rate is high, and probiotics in tunning, oligopeptides, glutamic acid, the content of lactic acid and vitamin are big, can
To improve animal body immunity, suppress the generation of disease of digestive tract, improve the fish brood amount of loach.
Preferably, the amino acid sequence of the active peptides added during bean pulp solid-state fermentation is:
HSHACASYSCRARRCAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.Bafillus natto is in solid-state
Exotoxin can be synthesized in fermentation process, influences its metabolism, the bioactive peptide of addition is combined with ectotoxic active site, had
Ectotoxic poisonous effect is reduced to effect, favorable environment is provided for solid state fermentation culture.
Preferably, the preparation method of feed is:Take white fish meal 12-16 parts, fermented bean dregs 20-24 parts, fish oil 3-5 parts,
Soybean oil 3-5 parts, Strong flour 40-45 parts, Choline Chloride 0.1-0.3 parts, calcium dihydrogen phosphate 0.8-1.2 parts, bile salt
0.1-0.2 parts are well mixed, by coarse-cracking machine and micronizer, sorting, granulation, curing, drying feed.Under the conditions of being somebody's turn to do
Prepared feed nutrition is comprehensive, and protein sources are mainly nonantigenic small peptide, are easily digested and assimilated by animal, reduces feed in water
Residual in body, reduce the ammonia-nitrogen content in water body, meet green requirement.
Compared with prior art, the advantage of the invention is that:Bosom ovum phase loach feed disclosed by the invention is with fermented bean dregs
For protein sources, with bafillus natto to bean pulp fermentation processing, make various antigenic components, ANFs in dregs of beans effective
Reduce and remove, the protein in dregs of beans is also broken down into substantial amounts of small peptide, and this nonantigenic small peptide is more easy to be absorbed by loach, energy
The fish brood amount of loach is enough significantly improved, while reduces residual of the feed in water body, reduces the ammonia-nitrogen content in water body, is accorded with
Close green requirement.
Embodiment
Below by embodiment, the present invention will be further described:
Embodiment 1:
Cherish ovum phase loach feed, the feed ingredient and its parts by weight are:12 parts of white fish meal, 20 parts of fermented bean dregs, 3 parts of fish oil, greatly
3 parts of soya-bean oil, 40 parts of Strong flour, 0.1 part of Choline Chloride, 0.8 part of calcium dihydrogen phosphate, 0.1 part of bile salt.The preparation of the feed
Method is:
1) activation culture:Bafillus natto is inoculated in slant medium first in a manner of sterile working, 30 DEG C are cultivated 2d,
The composition and its parts by weight of slant medium be:0.3 part of beef extract, 1 part of peptone, 0.5 part of sodium chloride, 2 parts of agar, water 90
Part;Then accessed from the ring bacterium of slant medium picking 1 in liquid activation medium, 34 DEG C, 170r/min shaken cultivation 6h, liquid
The composition and its parts by weight of activation medium be:1.5 parts of beef extract, 5 parts of peptone, 2.5 parts of sodium chloride, 400 parts of water.Used
Bafillus natto is bought in Chinese agriculture Culture Collection;
2) bean pulp solid-state fermentation:By the bafillus natto bacterium solution obtained by activation culture process with the access solid training of 10% inoculum concentration
Base is supported, 0.2 part of active peptides is added, 35 DEG C of culture 60h, obtains fermented bean dregs.The preparation method of solid medium is:Use plant
Pulverizer grinds dregs of beans, takes 7 parts of dregs of beans, 0.5 part of wheat bran and 2 parts of water mixing, stands 2h, gained mixture is placed in into blake bottle
In, 121 DEG C of sterilizing 25min, obtain solid medium.The amino acid sequence of active peptides is:
HSHACASYSCRARRCAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR;
3) preparation of feed:Take 12 parts of white fish meal, 20 parts of fermented bean dregs, 3 parts of fish oil, 3 parts of soybean oil, 40 parts of Strong flour, chlorine
Change 0.1 part of choline, 0.8 part of calcium dihydrogen phosphate, 0.1 part of bile salt is well mixed, by coarse-cracking machine and micronizer, divides
Choosing, granulation, curing, drying feed.Prepared feed nutrition is comprehensive under the conditions of this, and protein sources are mainly nonantigenic small
Peptide, easily digested and assimilated by animal, reduce residual of the feed in water body, reduce the ammonia-nitrogen content in water body, meet green
The requirement of environmental protection.
Embodiment 2:
Cherish ovum phase loach feed, the feed ingredient and its parts by weight are:16 parts of white fish meal, 24 parts of fermented bean dregs, 5 parts of fish oil, greatly
5 parts of soya-bean oil, 45 parts of Strong flour, 0.3 part of Choline Chloride, 1.2 parts of calcium dihydrogen phosphate, 0.2 part of bile salt.The preparation of the feed
Method is:It is activation culture first:Bafillus natto is inoculated in slant medium first in a manner of sterile working, 33 DEG C
3d is cultivated, the composition and its parts by weight of slant medium are:0.5 part of beef extract, 1.3 parts of peptone, 0.7 part of sodium chloride, agar 3
Part, 95 parts of water;Then accessed from the ring bacterium of slant medium picking 2 in liquid activation medium, 37 DEG C, 180r/min shaken cultivations
7h, the composition and its parts by weight of liquid activation medium are:2.5 parts of beef extract, 7 parts of peptone, 3.5 parts of sodium chloride, water 450
Part.Bafillus natto used is bought in Chinese agriculture Culture Collection.Under the conditions of being somebody's turn to do, the growth vigor of strain
Becoming strong, physiological character is more stable, can quickly obtain the purebred of certain amount and quality, shorten process cycle, and after being
Continuous bean pulp solid-state fermentation process provides sufficient amount of seed;Followed by bean pulp solid-state fermentation:By obtained by activation culture process
Bafillus natto bacterium solution solid medium is accessed with 10% inoculum concentration, add 0.4 part of active peptides, 37 DEG C of culture 70h,
Obtain fermented bean dregs.The preparation method of solid medium is:With plant pulverizer grind dregs of beans, take 9 parts of dregs of beans, 1.3 parts of wheat brans with
And 3 parts of water mixing, 2h is stood, gained mixture is placed in blake bottle, 121 DEG C of sterilizing 25min, obtains solid medium.This
Under part, solid medium utilization rate is high, and probiotics in tunning, oligopeptides, glutamic acid, the content of lactic acid and vitamin are big, can
To improve animal body immunity, suppress the generation of disease of digestive tract, improve the fish brood amount of loach;It is finally the preparation of feed:
Take 16 parts of white fish meal, 24 parts of fermented bean dregs, 5 parts of fish oil, 5 parts of soybean oil, 45 parts of Strong flour, 0.3 part of Choline Chloride, di(2-ethylhexyl)phosphate
1.2 parts of hydrogen calcium, 0.2 part of bile salt is well mixed, and by coarse-cracking machine and micronizer, sorting, granulation, curing, drying are
Obtain feed.Prepared feed nutrition is comprehensive under the conditions of this, and protein sources are mainly nonantigenic small peptide, are easily inhaled by animal digestion
Receive, reduce residual of the feed in water body, reduce the ammonia-nitrogen content in water body, meet green requirement.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Animal feed technological development Co., Ltd is full of by Pujiang County association
<120>Cherish ovum phase loach feed
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 57
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Ala Ser Tyr Ser Cys Arg Ala Arg Arg Cys Ala
1 5 10 15
His Cys Arg Arg Ala Arg Arg Cys Gly Tyr Tyr Val Ser His Arg Gly
20 25 30
Arg Cys Tyr Cys Arg Cys Leu Arg Cys Arg Val Leu His Pro Gly Lys
35 40 45
Leu Cys Val Cys Val Asn Cys Ser Arg
50 55
Claims (7)
1. cherish ovum phase loach feed, it is characterised in that:The composition and its parts by weight of the feed be:White fish meal 12-16 parts, fermentation
Dregs of beans 20-24 parts, fish oil 3-5 parts, soybean oil 3-5 parts, Strong flour 40-45 parts, Choline Chloride 0.1-0.3 parts, biphosphate
Calcium 0.8-1.2 parts, bile salt 0.1-0.2 parts.
2. bosom ovum phase loach feed according to claim 1, it is characterised in that:The preparation process of the fermented bean dregs is:
Activation culture, bean pulp solid-state fermentation.
3. bosom ovum phase loach feed according to claim 2, it is characterised in that:The process of the activation culture is:With nothing
Bafillus natto is inoculated in slant medium by the mode of bacterium operation, 30-33 DEG C of culture 2-3d, the composition of slant medium and
Its parts by weight is:Beef extract 0.3-0.5 parts, peptone 1-1.3 parts, sodium chloride 0.5-0.7 parts, agar 2-3 parts, water 90-95 parts;
Then accessed from slant medium picking 1-2 rings bacterium in liquid activation medium, 34-37 DEG C, 170-180r/min shaken cultivations
6-7h, the composition and its parts by weight of liquid activation medium are:Beef extract 1.5-2.5 parts, peptone 5-7 parts, sodium chloride 2.5-
3.5 parts, water 400-450 parts.
4. bosom ovum phase loach feed according to claim 2, it is characterised in that:The bean pulp solid-state fermentation process is:Will
Bafillus natto bacterium solution obtained by activation culture process accesses solid medium with 10% inoculum concentration, adds 0.2-0.4 parts
Active peptides, 35-37 DEG C of culture 60-70h, obtain fermented bean dregs.
5. bosom ovum phase loach feed according to claim 4, it is characterised in that:The amino acid sequence of the active peptides
For:HSHACASYSCRARRCAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.
6. bosom ovum phase loach feed according to claim 4, it is characterised in that:The preparation method of the solid medium
For:Dregs of beans is ground with plant pulverizer, 7-9 parts dregs of beans, 0.5-1.3 parts wheat bran and the mixing of 2-3 parts water is taken, 2h is stood, by institute
Obtain mixture to be placed in blake bottle, 121 DEG C of sterilizing 25min, obtain solid medium.
7. bosom ovum phase loach feed according to claim 1, it is characterised in that:The preparation method of the feed is:Take white
Fish meal 12-16 parts, fermented bean dregs 20-24 parts, fish oil 3-5 parts, soybean oil 3-5 parts, Strong flour 40-45 parts, Choline Chloride
0.1-0.3 parts, calcium dihydrogen phosphate 0.8-1.2 parts, bile salt 0.1-0.2 parts are well mixed, by coarse-cracking machine and ultramicro grinding
Machine, sorting, granulation, curing, drying feed.
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CN201710640256.2A CN107348126A (en) | 2017-07-31 | 2017-07-31 | Cherish ovum phase loach feed |
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Citations (6)
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---|---|---|---|---|
CN101283737A (en) * | 2008-04-09 | 2008-10-15 | 齐齐哈尔大学 | Fermented feed rich in probiotics and its preparation method |
CN103734530A (en) * | 2013-12-31 | 2014-04-23 | 佛山市信豚生物科技有限公司 | Feed inducing agent for aquatic feed |
CN103859151A (en) * | 2012-12-18 | 2014-06-18 | 青岛中仁药业有限公司 | Production technology of bacillus natto fermented soybean meal |
CN104000050A (en) * | 2014-06-18 | 2014-08-27 | 通威股份有限公司 | Loach settling-type puffing mixed feed and preparing method thereof |
CN104093734A (en) * | 2011-12-21 | 2014-10-08 | 明尼苏达大学董事会 | Cationic peptides and use of such peptides for inhibiting exotoxin production |
CN105660992A (en) * | 2014-11-22 | 2016-06-15 | 天津君合心科技发展有限公司 | Preparation method of fermented feed easy to absorb by animals |
-
2017
- 2017-07-31 CN CN201710640256.2A patent/CN107348126A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101283737A (en) * | 2008-04-09 | 2008-10-15 | 齐齐哈尔大学 | Fermented feed rich in probiotics and its preparation method |
CN104093734A (en) * | 2011-12-21 | 2014-10-08 | 明尼苏达大学董事会 | Cationic peptides and use of such peptides for inhibiting exotoxin production |
CN103859151A (en) * | 2012-12-18 | 2014-06-18 | 青岛中仁药业有限公司 | Production technology of bacillus natto fermented soybean meal |
CN103734530A (en) * | 2013-12-31 | 2014-04-23 | 佛山市信豚生物科技有限公司 | Feed inducing agent for aquatic feed |
CN104000050A (en) * | 2014-06-18 | 2014-08-27 | 通威股份有限公司 | Loach settling-type puffing mixed feed and preparing method thereof |
CN105660992A (en) * | 2014-11-22 | 2016-06-15 | 天津君合心科技发展有限公司 | Preparation method of fermented feed easy to absorb by animals |
Non-Patent Citations (1)
Title |
---|
杨玉红等: "《生命科学综合实验指导》", 31 July 2016, 东北大学出版社 * |
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Application publication date: 20171117 |