CN107439842A - Penaeus Vannmei growth-promoting feed - Google Patents
Penaeus Vannmei growth-promoting feed Download PDFInfo
- Publication number
- CN107439842A CN107439842A CN201710638690.7A CN201710638690A CN107439842A CN 107439842 A CN107439842 A CN 107439842A CN 201710638690 A CN201710638690 A CN 201710638690A CN 107439842 A CN107439842 A CN 107439842A
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- CN
- China
- Prior art keywords
- parts
- peanut meal
- penaeus vannmei
- feed
- growth
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses Penaeus Vannmei growth-promoting feed, the composition and its parts by weight of the feed are：12 16 parts of fish meal, 20 24 parts of fermented peanut meal, 35 parts of fish oil, 25 parts of soyabean lecithin oil, 40 45 parts of flour, 0.1 0.3 parts of Choline Chloride, 0.8 1.2 parts of brewer's yeast, 0.1 0.2 parts of bile salt.Have the beneficial effect that：The present invention is with bacillus subtilis to peanut meal fermentation process, various antigenic components, ANFs in peanut meal is set to be can be effectively reduced removal, protein in peanut meal is also broken down into substantial amounts of small peptide, this nonantigenic small peptide is more easy to be absorbed by Penaeus Vannmei, the growth of Penaeus Vannmei can be remarkably promoted, residual of the feed in water body is reduced simultaneously, reduces the ammonia-nitrogen content in water body, meets green requirement.
Description
Technical field
The present invention relates to aquatic feeds field, more particularly, to Penaeus Vannmei growth-promoting feed.
Background technology
Penaeus Vannmei belongs to Penaeidae, scientific name Environment of Litopenaeus vannamei Low, is three big species of world's shrimps in culture yield highest
One of.The thin body fertilizer of Penaeus Vannmei shell, Fresh ＆ Tender in Texture, dressing percentage is up to more than 65%, and nutritious, depth is joyous by domestic and international market
Meet；The speed of growth is fast, and 60 days i.e. up to listing specification；Proper salinity level is wide (0-40%), can take pure fresh water, brackish water, sea
The a variety of aquaculture models of water, can grow from natural waterses to freshwater, so as to break territory restriction, and have high temperature resistant, resist
Sick power is strong, is the fine quality of " sea shrimp is light to support ", is the shrimps in culture for having very much development potentiality.Culture of Penaeus vannamei is China sea
The pillar industry of water cultivation, economic benefit is good, and development prospect is wide, and culture of Penaeus vannamei is intensive and the life of scale
Production has dependent on penaeus vannamei boone feed industrial expansion.
Prior art such as Authorization Notice No. is the B of CN 101828644 Chinese invention patent, discloses a kind of vannamei boone pair
Shrimp feed, nutrition is balanced comprehensively, improves the cultivation profit of Environment of Litopenaeus vannamei Low, but the feed is non-digestible, and Yi Shui
Remained in body, in turn result in the pollution-free of environment.
The content of the invention
It is an object of the invention to provide feed absorption easy to digest prepared by Penaeus Vannmei growth-promoting feed, the method, energy
Penaeus Vannmei growth is enough remarkably promoted, and it is small to water pollution.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Penaeus Vannmei growth-promoting feed,
The composition and its parts by weight of the feed be：Fish meal 12-16 parts, fermented peanut meal 20-24 parts, fish oil 3-5 parts, soyabean lecithin oil 2-
5 parts, flour 40-45 parts, Choline Chloride 0.1-0.3 parts, brewer's yeast 0.8-1.2 parts, bile salt 0.1-0.2 parts.Prepared
The cost of raw material is cheap used in journey, easily stored, and the ratio between composition is moderate, and prepared feed palatability is good, easily
Absorption is digested, Penaeus Vannmei growth can be remarkably promoted, and is remained less in water body, it is small to water pollution.
Preferably, the preparation method of fermented peanut meal is：Activation culture, peanut meal fermentation.With bacillus subtilis pair
Peanut meal fermentation process, various antigenic components, ANFs in peanut meal is set to be can be effectively reduced removal, the egg in peanut meal
White matter is also broken down into substantial amounts of small peptide, and this nonantigenic small peptide is more easy to be absorbed by Penaeus Vannmei, can remarkably promote south
The growth of penaeus vannamei.Bacillus subtilis used is bought in Chinese agriculture Culture Collection.
Preferably, activation culture process is：Bacillus subtilis is inoculated in inclined-plane culture in a manner of sterile working
Base, 29-33 DEG C of culture 2-3d, the composition and its parts by weight of slant medium are：Beef extract 0.2-0.5 parts, peptone 1-1.4
Part, sodium chloride 0.4-0.7 parts, agar 2-3 parts, water 90-95 parts；Then lived from slant medium picking 1-2 rings bacterium access liquid
Change in culture medium, 32-37 DEG C, 170-180r/min shaken cultivation 6-7h, the composition and its parts by weight of liquid activation medium are：
Beef extract 1.4-2.5 parts, peptone 5-7 parts, sodium chloride 2.4-3.5 parts, water 400-450 parts.Under the conditions of being somebody's turn to do, the growth of strain is lived
Power becomes strong, and physiological character is more stable, can quickly obtain purebred, the shortening process cycle of certain amount and quality, and is
Follow-up peanut meal fermentation process provides sufficient amount of seed.
Preferably, peanut meal fermentation process is：By the bacillus subtilis bacterium solution obtained by activation culture process with 10%
Inoculum concentration accesses peanut meal fermentation substrate, adds 0.2-0.5 part active peptides, 30-37 DEG C of culture 60-70h, obtains fermenting peanut
The dregs of rice.The preparation method of peanut meal fermentation substrate is：Peanut meal is ground with plant pulverizer, takes 7-9 parts peanut meal, 0.5-1.3 parts
Wheat bran and the mixing of 2-3 parts water, stand 2h, gained mixture are placed in blake bottle, 121 DEG C of sterilizing 25min, obtain peanut meal hair
Ferment matrix.Under the conditions of being somebody's turn to do, peanut meal fermentation substrate utilization rate is high, probiotics, oligopeptides, glutamic acid, lactic acid and dimension in tunning
The content of raw element is big, can improve animal body immunity, suppress the generation of disease of digestive tract, promote the life of Penaeus Vannmei
It is long.
Preferably, the amino acid sequence of the active peptides added in peanut meal fermentation process is：
HSHARARRCGYYVSKAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.Bacillus subtilis is in solid-state
Exotoxin can be synthesized in fermentation process, influences its metabolism, the bioactive peptide of addition is combined with ectotoxic active site, had
Ectotoxic poisonous effect is reduced to effect, favorable environment is provided for solid state fermentation culture.
Preferably, the preparation process of feed is：Take fish meal 12-16 parts, fermented peanut meal 20-24 parts, fish oil 3-5 parts,
Soyabean lecithin oil 2-5 parts, flour 40-45 parts, Choline Chloride 0.1-0.3 parts, brewer's yeast 0.8-1.2 parts, bile salt 0.1-
0.2 part well mixed, by coarse-cracking machine and micronizer, sorting, granulation, curing, drying feed.It is made under the conditions of being somebody's turn to do
Standby feed nutrition is comprehensive, and protein sources are mainly nonantigenic small peptide, are easily digested and assimilated by animal, reduces feed in water body
Residual, reduce the ammonia-nitrogen content in water body, meet green requirement.
Compared with prior art, the advantage of the invention is that：The present invention prepares feed using fermented peanut meal as protein sources, with
Bacillus subtilis is can be effectively reduced various antigenic components, ANFs in peanut meal peanut meal fermentation process
Removing, the protein in peanut meal is also broken down into substantial amounts of small peptide, and this nonantigenic small peptide is more easy to be absorbed by Penaeus Vannmei,
The growth of Penaeus Vannmei can be remarkably promoted, while reduces residual of the feed in water body, reduces the ammonia nitrogen in water body
Content, meet green requirement.
Embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
Penaeus Vannmei growth-promoting feed, the feed ingredient and its parts by weight are：12 parts of fish meal, 20 parts of fermented peanut meal, fish oil 3
Part, 2 parts of soyabean lecithin oil, 40 parts of flour, 0.1 part of Choline Chloride, 0.8 part of brewer's yeast, 0.1 part of bile salt.The system of feed
It is standby to comprise the following steps：
1) activation culture：Bacillus subtilis is inoculated in slant medium first in a manner of sterile working, 29 DEG C are cultivated 2d,
The composition and its parts by weight of slant medium be：0.2 part of beef extract, 1 part of peptone, 0.4 part of sodium chloride, 2 parts of agar, water 90
Part；Then accessed from the ring bacterium of slant medium picking 1 in liquid activation medium, 32 DEG C, 170r/min shaken cultivation 6h, liquid
The composition and its parts by weight of activation medium be：1.4 parts of beef extract, 5 parts of peptone, 2.4 parts of sodium chloride, 400 parts of water.Used
Bacillus subtilis is bought in Chinese agriculture Culture Collection；
2) peanut meal ferments：By the bacillus subtilis bacterium solution obtained by activation culture process with 10% inoculum concentration access peanut meal hair
Ferment matrix, 0.2 part of active peptides is added, 30 DEG C of culture 60h, obtains fermented peanut meal.The preparation method of peanut meal fermentation substrate
For：Plant pulverizer grinds peanut meal, takes 7 parts of peanut meals, 0.5 part of wheat bran and 2 parts of water mixing, stands 2h, gained is mixed
Thing is placed in blake bottle, 121 DEG C of sterilizing 25min, obtains peanut meal fermentation substrate.The amino acid sequence of active peptides is：
HSHARARRCGYYVSKAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR；
3) preparation of feed：Take 12 parts of fish meal, 20 parts of fermented peanut meal, 3 parts of fish oil, 2 parts of soyabean lecithin oil, 40 parts of flour, chlorine
Change 0.1 part of choline, 0.8 part of brewer's yeast, 0.1 part of bile salt is well mixed, by coarse-cracking machine and micronizer, sorting,
Granulation, curing, drying feed.Prepared feed nutrition is comprehensive under the conditions of this, and protein sources are mainly nonantigenic small peptide,
Easily digested and assimilated by animal, reduce residual of the feed in water body, reduce the ammonia-nitrogen content in water body, meet green
Requirement.
Embodiment 2：
Penaeus Vannmei growth-promoting feed, the feed ingredient and its parts by weight are：16 parts of fish meal, 24 parts of fermented peanut meal, fish oil 5
Part, 5 parts of soyabean lecithin oil, 45 parts of flour, 0.3 part of Choline Chloride, 1.2 parts of brewer's yeast, 0.2 part of bile salt.The system of feed
It is standby to comprise the following steps：It is first activation culture：Bacillus subtilis is inoculated in inclined-plane culture in a manner of sterile working first
Base, 33 DEG C of culture 3d, the composition and its parts by weight of slant medium are：0.5 part of beef extract, 1.4 parts of peptone, sodium chloride 0.7
Part, 3 parts of agar, 95 parts of water；Then accessed from the ring bacterium of slant medium picking 2 in liquid activation medium, 37 DEG C, 180r/min
Shaken cultivation 7h, the composition and its parts by weight of liquid activation medium are：2.5 parts of beef extract, 7 parts of peptone, sodium chloride 3.5
Part, 450 parts of water.Bacillus subtilis used is bought in Chinese agriculture Culture Collection.Under the conditions of being somebody's turn to do, strain
Growth vigor becomes strong, and physiological character is more stable, can quickly obtain purebred, the shortening technique week of certain amount and quality
Phase, and provide sufficient amount of seed for follow-up peanut meal fermentation process；Fermented followed by peanut meal：By activation culture process
The bacillus subtilis bacterium solution of gained accesses peanut meal fermentation substrate with 10% inoculum concentration, adds 0.5 part of active peptides, 37 DEG C
70h is cultivated, obtains fermented peanut meal.The preparation method of peanut meal fermentation substrate is：Plant pulverizer grinds peanut meal, takes 9 parts of flowers
The raw dregs of rice, 1.3 parts of wheat brans and 3 parts of water mixing, stand 2h, gained mixture are placed in blake bottle, 121 DEG C of sterilizing 25min, obtain
Peanut meal fermentation substrate.Should under the conditions of, peanut meal fermentation substrate utilization rate is high, probiotics in tunning, oligopeptides, glutamic acid,
The content of lactic acid and vitamin is big, can improve animal body immunity, suppresses the generation of disease of digestive tract, promotes South America right in vain
The growth of shrimp；It is finally the preparation of feed：Take 16 parts of fish meal, 24 parts of fermented peanut meal, 5 parts of fish oil, 5 parts of soyabean lecithin oil, face
45 parts of powder, 0.3 part of Choline Chloride, 1.2 parts of brewer's yeast, 0.2 part of bile salt is well mixed, by coarse-cracking machine and ultramicro grinding
Machine, sorting, granulation, curing, drying feed.Prepared feed nutrition is comprehensive under the conditions of this, and protein sources are mainly nonreactive
Former small peptide, is easily digested and assimilated by animal, reduces residual of the feed in water body, reduce the ammonia-nitrogen content in water body, accords with
Close green requirement.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Animal feed technological development Co., Ltd is full of by Pujiang County association
<120>Penaeus Vannmei growth-promoting feed
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 57
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Arg Ala Arg Arg Cys Gly Tyr Tyr Val Ser Lys Ala
1 5 10 15
His Cys Arg Arg Ala Arg Arg Cys Gly Tyr Tyr Val Ser His Arg Gly
20 25 30
Arg Cys Tyr Cys Arg Cys Leu Arg Cys Arg Val Leu His Pro Gly Lys
35 40 45
Leu Cys Val Cys Val Asn Cys Ser Arg
50 55
Claims (7)
1. Penaeus Vannmei growth-promoting feed, it is characterised in that：The composition and its parts by weight of the feed be：Fish meal 12-16 parts,
Fermented peanut meal 20-24 parts, fish oil 3-5 parts, soyabean lecithin oil 2-5 parts, flour 40-45 parts, Choline Chloride 0.1-0.3 parts, beer
Brewer yeast 0.8-1.2 parts, bile salt 0.1-0.2 parts.
2. Penaeus Vannmei growth-promoting feed according to claim 1, it is characterised in that：The preparation of the fermented peanut meal
Method is：Activation culture, peanut meal fermentation.
3. Penaeus Vannmei growth-promoting feed according to claim 2, it is characterised in that：The peanut meal fermentation process
For：Bacillus subtilis bacterium solution obtained by activation culture process is accessed into peanut meal fermentation substrate with 10% inoculum concentration, added
0.2-0.5 part active peptides, 30-37 DEG C of culture 60-70h, obtain fermented peanut meal.
4. Penaeus Vannmei growth-promoting feed according to claim 2, it is characterised in that：The process of the activation culture
For：Bacillus subtilis is inoculated in slant medium, 29-33 DEG C of culture 2-3d, slant medium in a manner of sterile working
Composition and its parts by weight be：Beef extract 0.2-0.5 parts, peptone 1-1.4 parts, sodium chloride 0.4-0.7 parts, agar 2-3 parts, water
90-95 parts；Then accessed from slant medium picking 1-2 rings bacterium in liquid activation medium, 32-37 DEG C, 170-180r/min
Shaken cultivation 6-7h, the composition and its parts by weight of liquid activation medium are：Beef extract 1.4-2.5 parts, peptone 5-7 parts, chlorine
Change sodium 2.4-3.5 parts, water 400-450 parts.
5. Penaeus Vannmei growth-promoting feed according to claim 1, it is characterised in that：The preparation method of the feed
For：Take fish meal 12-16 parts, fermented peanut meal 20-24 parts, fish oil 3-5 parts, soyabean lecithin oil 2-5 parts, flour 40-45 parts, chlorination
Choline 0.1-0.3 parts, brewer's yeast 0.8-1.2 parts, bile salt 0.1-0.2 parts are well mixed, by coarse-cracking machine and Ultramicro-powder
Broken machine, sorting, granulation, curing, drying feed.
6. Penaeus Vannmei growth-promoting feed according to claim 3, it is characterised in that：The amino acid of the active peptides
Sequence is：HSHARARRCGYYVSKAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.
7. Penaeus Vannmei growth-promoting feed according to claim 3, it is characterised in that：The peanut meal fermentation substrate
Preparation method is：Peanut meal is ground with plant pulverizer, takes 7-9 parts peanut meal, 0.5-1.3 parts wheat bran and the mixing of 2-3 parts water,
2h is stood, gained mixture is placed in blake bottle, 121 DEG C of sterilizing 25min, obtains peanut meal fermentation substrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710638690.7A CN107439842A (en) | 2017-07-31 | 2017-07-31 | Penaeus Vannmei growth-promoting feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710638690.7A CN107439842A (en) | 2017-07-31 | 2017-07-31 | Penaeus Vannmei growth-promoting feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107439842A true CN107439842A (en) | 2017-12-08 |
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ID=60490538
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710638690.7A Pending CN107439842A (en) | 2017-07-31 | 2017-07-31 | Penaeus Vannmei growth-promoting feed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113412891A (en) * | 2021-07-13 | 2021-09-21 | 江苏艾生牧饲料有限公司 | Penaeus vannamei larva breeding feed and preparation process and preparation device thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828644A (en) * | 2010-05-11 | 2010-09-15 | 中山大学 | Feed for litopenaeus vannamei |
| CN104093734A (en) * | 2011-12-21 | 2014-10-08 | 明尼苏达大学董事会 | Cationic peptides and use of such peptides for inhibiting exotoxin production |
| CN104757320A (en) * | 2015-04-10 | 2015-07-08 | 烟台大乐饲料有限公司 | Functional compound feed special for greenhouse cultivation of litopenaeus vannamei |
-
2017
- 2017-07-31 CN CN201710638690.7A patent/CN107439842A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828644A (en) * | 2010-05-11 | 2010-09-15 | 中山大学 | Feed for litopenaeus vannamei |
| CN104093734A (en) * | 2011-12-21 | 2014-10-08 | 明尼苏达大学董事会 | Cationic peptides and use of such peptides for inhibiting exotoxin production |
| CN104757320A (en) * | 2015-04-10 | 2015-07-08 | 烟台大乐饲料有限公司 | Functional compound feed special for greenhouse cultivation of litopenaeus vannamei |
Non-Patent Citations (1)
| Title |
|---|
| 孙杰等: "枯草芽孢杆菌发酵制备花生粕饲料条件优化", 《核农学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113412891A (en) * | 2021-07-13 | 2021-09-21 | 江苏艾生牧饲料有限公司 | Penaeus vannamei larva breeding feed and preparation process and preparation device thereof |
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Application publication date: 20171208 |