CN107467347A - The preparation method of Penaeus Vannmei growth-promoting feed - Google Patents
The preparation method of Penaeus Vannmei growth-promoting feed Download PDFInfo
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- CN107467347A CN107467347A CN201710638731.2A CN201710638731A CN107467347A CN 107467347 A CN107467347 A CN 107467347A CN 201710638731 A CN201710638731 A CN 201710638731A CN 107467347 A CN107467347 A CN 107467347A
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- parts
- preparation
- peanut meal
- penaeus vannmei
- feed
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses the preparation method of Penaeus Vannmei growth-promoting feed, including activation culture, peanut meal fermentation, the preparation of feed.Have the beneficial effect that：Penaeus Vannmei growth-promoting feed prepared by the present invention is using fermented peanut meal as protein sources, with bacillus subtilis to peanut meal fermentation process, various antigenic components, ANFs in peanut meal is set to be can be effectively reduced removal, protein in peanut meal is also broken down into substantial amounts of small peptide, this nonantigenic small peptide is more easy to be absorbed by Penaeus Vannmei, the growth of Penaeus Vannmei can be remarkably promoted, reduce residual of the feed in water body simultaneously, reduce the ammonia-nitrogen content in water body, meet green requirement.
Description
Technical field
The present invention relates to aquatic feeds field, more particularly, to the preparation method of Penaeus Vannmei growth-promoting feed.
Background technology
Penaeus Vannmei belongs to Penaeidae, scientific name Environment of Litopenaeus vannamei Low, is three big species of world's shrimps in culture yield highest
One of.The thin body fertilizer of Penaeus Vannmei shell, Fresh ＆ Tender in Texture, dressing percentage is up to more than 65%, and nutritious, depth is joyous by domestic and international market
Meet；The speed of growth is fast, and 60 days i.e. up to listing specification；Proper salinity level is wide (0-40%), can take pure fresh water, brackish water, sea
The a variety of aquaculture models of water, can grow from natural waterses to freshwater, so as to break territory restriction, and have high temperature resistant, resist
Sick power is strong, is the fine quality of " sea shrimp is light to support ", is the shrimps in culture for having very much development potentiality.Culture of Penaeus vannamei is China sea
The pillar industry of water cultivation, economic benefit is good, and development prospect is wide, and culture of Penaeus vannamei is intensive and the life of scale
Production has dependent on penaeus vannamei boone feed industrial expansion.
Prior art such as Authorization Notice No. is the B of CN 101828644 Chinese invention patent, discloses a kind of vannamei boone pair
Shrimp feed, nutrition is balanced comprehensively, improves the cultivation profit of Environment of Litopenaeus vannamei Low, but the feed is non-digestible, and Yi Shui
Remained in body, in turn result in the pollution-free of environment.
The content of the invention
It is an object of the invention to provide the preparation method of Penaeus Vannmei growth-promoting feed, feed prepared by the method easily disappears
Change and absorb, Penaeus Vannmei growth can be remarkably promoted, and it is small to water pollution.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Penaeus Vannmei growth-promoting feed
Preparation method, including activation culture, peanut meal fermentation, the preparation of feed.The cost of raw material is cheap used in preparation process,
Easily stored, the ratio between composition is moderate, and prepared feed palatability is good, is easily digested absorption, can remarkably promote south
Penaeus vannamei grows, and is remained less in water body, small to water pollution.
Preferably, activation culture process is：Bacillus subtilis is inoculated in inclined-plane culture in a manner of sterile working
Base, 29-33 DEG C of culture 2-3d, the composition and its parts by weight of slant medium are：Beef extract 0.2-0.5 parts, peptone 1-1.4
Part, sodium chloride 0.4-0.7 parts, agar 2-3 parts, water 90-95 parts；Then lived from slant medium picking 1-2 rings bacterium access liquid
Change in culture medium, 32-37 DEG C, 170-180r/min shaken cultivation 6-7h, the composition and its parts by weight of liquid activation medium are：
Beef extract 1.4-2.5 parts, peptone 5-7 parts, sodium chloride 2.4-3.5 parts, water 400-450 parts.Bacillus subtilis purchase used
Buy in Chinese agriculture Culture Collection.Under the conditions of being somebody's turn to do, the growth vigor of strain becomes strong, and physiological character is more stable,
Purebred, the shortening process cycle of certain amount and quality can be quickly obtained, and is provided for follow-up peanut meal fermentation process
Sufficient amount of seed.
Preferably, peanut meal fermentation process is：By the bacillus subtilis bacterium solution obtained by activation culture process with 10%
Inoculum concentration accesses peanut meal fermentation substrate, adds 0.2-0.5 part active peptides, 30-37 DEG C of culture 60-70h, obtains fermenting peanut
The dregs of rice.The preparation method of peanut meal fermentation substrate is：Peanut meal is ground with plant pulverizer, takes 7-9 parts peanut meal, 0.5-1.3 parts
Wheat bran and the mixing of 2-3 parts water, stand 2h, gained mixture are placed in blake bottle, 121 DEG C of sterilizing 25min, obtain peanut meal hair
Ferment matrix.Under the conditions of being somebody's turn to do, peanut meal fermentation substrate utilization rate is high, probiotics, oligopeptides, glutamic acid, lactic acid and dimension in tunning
The content of raw element is big, can improve animal body immunity, suppress the generation of disease of digestive tract, promote the life of Penaeus Vannmei
It is long.
Preferably, the amino acid sequence of the active peptides added in peanut meal fermentation process is：
HSHACARRCGYYVSKAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.Bacillus subtilis is in solid-state
Exotoxin can be synthesized in fermentation process, influences its metabolism, the bioactive peptide of addition is combined with ectotoxic active site, had
Ectotoxic poisonous effect is reduced to effect, favorable environment is provided for solid state fermentation culture.
Preferably, the preparation process of feed is：Take fish meal 12-16 parts, fermented peanut meal 20-24 parts, fish oil 3-5 parts,
Soyabean lecithin oil 2-5 parts, flour 40-45 parts, Choline Chloride 0.1-0.3 parts, brewer's yeast 0.8-1.2 parts, bile salt 0.1-
0.2 part well mixed, by coarse-cracking machine and micronizer, sorting, granulation, curing, drying feed.It is made under the conditions of being somebody's turn to do
Standby feed nutrition is comprehensive, and protein sources are mainly nonantigenic small peptide, are easily digested and assimilated by animal, reduces feed in water body
Residual, reduce the ammonia-nitrogen content in water body, meet green requirement.
Compared with prior art, the advantage of the invention is that：The process that the present invention prepares feed includes activation culture, peanut
The dregs of rice ferment and the preparation of feed, and the feed is using fermented peanut meal as protein sources, with bacillus subtilis to peanut meal fermentation process,
Various antigenic components, ANFs in peanut meal is set to be can be effectively reduced removal, the protein in peanut meal is also broken down into
Substantial amounts of small peptide, this nonantigenic small peptide are more easy to be absorbed by Penaeus Vannmei, can remarkably promote the growth of Penaeus Vannmei,
Residual of the feed in water body is reduced simultaneously, reduces the ammonia-nitrogen content in water body, meets green requirement.
Embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
The preparation method of Penaeus Vannmei growth-promoting feed, comprises the following steps：
1) activation culture：Bacillus subtilis is inoculated in slant medium first in a manner of sterile working, 29 DEG C are cultivated 2d,
The composition and its parts by weight of slant medium be：0.2 part of beef extract, 1 part of peptone, 0.4 part of sodium chloride, 2 parts of agar, water 90
Part；Then accessed from the ring bacterium of slant medium picking 1 in liquid activation medium, 32 DEG C, 170r/min shaken cultivation 6h, liquid
The composition and its parts by weight of activation medium be：1.4 parts of beef extract, 5 parts of peptone, 2.4 parts of sodium chloride, 400 parts of water.Used
Bacillus subtilis is bought in Chinese agriculture Culture Collection；
2) peanut meal ferments：By the bacillus subtilis bacterium solution obtained by activation culture process with 10% inoculum concentration access peanut meal hair
Ferment matrix, 0.2 part of active peptides is added, 30 DEG C of culture 60h, obtains fermented peanut meal.The preparation method of peanut meal fermentation substrate
For：Plant pulverizer grinds peanut meal, takes 7 parts of peanut meals, 0.5 part of wheat bran and 2 parts of water mixing, stands 2h, gained is mixed
Thing is placed in blake bottle, 121 DEG C of sterilizing 25min, obtains peanut meal fermentation substrate.The amino acid sequence of active peptides is：
HSHACARRCGYYVSKAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR；
3) preparation of feed：Take 12 parts of fish meal, 20 parts of fermented peanut meal, 3 parts of fish oil, 2 parts of soyabean lecithin oil, 40 parts of flour, chlorine
Change 0.1 part of choline, 0.8 part of brewer's yeast, 0.1 part of bile salt is well mixed, by coarse-cracking machine and micronizer, sorting,
Granulation, curing, drying feed.Prepared feed nutrition is comprehensive under the conditions of this, and protein sources are mainly nonantigenic small peptide,
Easily digested and assimilated by animal, reduce residual of the feed in water body, reduce the ammonia-nitrogen content in water body, meet green
Requirement.
Embodiment 2：
The preparation method of Penaeus Vannmei growth-promoting feed, comprises the following steps：It is activation culture first：First with sterile working
Mode bacillus subtilis is inoculated in slant medium, 33 DEG C of culture 3d, the composition and its parts by weight of slant medium are：
0.5 part of beef extract, 1.4 parts of peptone, 0.7 part of sodium chloride, 3 parts of agar, 95 parts of water；Then from the ring bacterium of slant medium picking 2
Access in liquid activation medium, 37 DEG C, 180r/min shaken cultivation 7h, the composition and its parts by weight of liquid activation medium
For：2.5 parts of beef extract, 7 parts of peptone, 3.5 parts of sodium chloride, 450 parts of water.Bacillus subtilis used is bought in Chinese agriculture
Culture Collection.Under the conditions of being somebody's turn to do, the growth vigor of strain becomes strong, and physiological character is more stable, can quickly obtain
Purebred, the shortening process cycle of certain amount and quality is obtained, and sufficient amount of kind is provided for follow-up peanut meal fermentation process
Son；Fermented followed by peanut meal：Bacillus subtilis bacterium solution obtained by activation culture process is accessed into peanut with 10% inoculum concentration
Dregs of rice fermentation substrate, 0.5 part of active peptides is added, 37 DEG C of culture 70h, obtains fermented peanut meal.The preparation of peanut meal fermentation substrate
Method is：Plant pulverizer grinds peanut meal, takes 9 parts of peanut meals, 1.3 parts of wheat brans and 3 parts of water mixing, 2h is stood, by gained
Mixture is placed in blake bottle, 121 DEG C of sterilizing 25min, obtains peanut meal fermentation substrate.Under the conditions of being somebody's turn to do, peanut meal fermentation substrate profit
High with rate, probiotics in tunning, oligopeptides, glutamic acid, the content of lactic acid and vitamin are big, can improve animal body and be immunized
Power, suppress the generation of disease of digestive tract, promote the growth of Penaeus Vannmei；It is finally the preparation of feed：16 parts of fish meal is taken, is fermented
24 parts of peanut meal, 5 parts of fish oil, 5 parts of soyabean lecithin oil, 45 parts of flour, 0.3 part of Choline Chloride, 1.2 parts of brewer's yeast, bile acid
0.2 part of salt is well mixed, by coarse-cracking machine and micronizer, sorting, granulation, curing, drying feed.Institute under the conditions of being somebody's turn to do
The feed nutrition of preparation is comprehensive, and protein sources are mainly nonantigenic small peptide, are easily digested and assimilated by animal, reduces feed in water body
In residual, reduce the ammonia-nitrogen content in water body, meet green requirement.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Lanxi City You Ze feed technologies Services Co., Ltd
<120>The preparation method of Penaeus Vannmei growth-promoting feed
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 57
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Ala Arg Arg Cys Gly Tyr Tyr Val Ser Lys Ala
1 5 10 15
His Cys Arg Arg Ala Arg Arg Cys Gly Tyr Tyr Val Ser His Arg Gly
20 25 30
Arg Cys Tyr Cys Arg Cys Leu Arg Cys Arg Val Leu His Pro Gly Lys
35 40 45
Leu Cys Val Cys Val Asn Cys Ser Arg
50 55
Claims (7)
1. the preparation method of Penaeus Vannmei growth-promoting feed, including activation culture, peanut meal fermentation, the preparation of feed, it is special
Sign is：The peanut meal fermentation process is：By the bacillus subtilis bacterium solution obtained by activation culture process with 10% inoculum concentration
Peanut meal fermentation substrate is accessed, 0.2-0.5 part active peptides is added, 30-37 DEG C of culture 60-70h, obtains fermented peanut meal.
2. the preparation method of Penaeus Vannmei growth-promoting feed according to claim 1, it is characterised in that：The activation training
Foster process is：Bacillus subtilis is inoculated in slant medium, 29-33 DEG C of culture 2-3d in a manner of sterile working；Connect
From slant medium picking 1-2 rings bacterium and access in liquid activation medium, 32-37 DEG C, 170-180r/min shaken cultivations 6-
7h。
3. the preparation method of Penaeus Vannmei growth-promoting feed according to claim 1, it is characterised in that：The peanut meal
The preparation method of fermentation substrate is：Peanut meal is ground with plant pulverizer, takes 7-9 parts peanut meal, 0.5-1.3 parts wheat bran and 2-
3 parts of water mixing, stand 2h, gained mixture are placed in blake bottle, 121 DEG C of sterilizing 25min, obtain peanut meal fermentation substrate.
4. the preparation method of Penaeus Vannmei growth-promoting feed according to claim 1, it is characterised in that：Described activity
The amino acid sequence of polypeptide is：HSHACARRCGYYVSKAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.
5. the preparation method of Penaeus Vannmei growth-promoting feed according to claim 2, it is characterised in that：The inclined-plane training
Support base composition and its parts by weight be：Beef extract 0.2-0.5 parts, peptone 1-1.4 parts, sodium chloride 0.4-0.7 parts, agar 2-3
Part, water 90-95 parts.
6. the preparation method of Penaeus Vannmei growth-promoting feed according to claim 2, it is characterised in that：The liquid is lived
Change culture medium composition and its parts by weight be：Beef extract 1.4-2.5 parts, peptone 5-7 parts, sodium chloride 2.4-3.5 parts, water 400-
450 parts.
7. the preparation method of Penaeus Vannmei growth-promoting feed according to claim 1, it is characterised in that：The feed
Preparation method is：Take fish meal 12-16 parts, fermented peanut meal 20-24 parts, fish oil 3-5 parts, soyabean lecithin oil 2-5 parts, flour 40-
45 parts, Choline Chloride 0.1-0.3 parts, brewer's yeast 0.8-1.2 parts, bile salt 0.1-0.2 parts are well mixed, by coarse-cracking machine
And micronizer, sorting, granulation, curing, drying feed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710638731.2A CN107467347A (en) | 2017-07-31 | 2017-07-31 | The preparation method of Penaeus Vannmei growth-promoting feed |
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| CN201710638731.2A CN107467347A (en) | 2017-07-31 | 2017-07-31 | The preparation method of Penaeus Vannmei growth-promoting feed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108514045A (en) * | 2018-03-12 | 2018-09-11 | 大连民族大学 | One kind having purification of water quality and the immunocompetent fancy carp microbial inoculum bait of raising and preparation method thereof |
| CN110637928A (en) * | 2019-09-26 | 2020-01-03 | 海南农垦南金农场有限公司 | Method for preparing marine aquatic feed by taking jack fruit seeds as raw materials |
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| CN101828644A (en) * | 2010-05-11 | 2010-09-15 | 中山大学 | Feed for litopenaeus vannamei |
| CN102008006A (en) * | 2009-09-08 | 2011-04-13 | 中国农业科学院饲料研究所 | Preparation method of polypeptide for fermented feed with molecular weight of less than 15KDa |
| CN104093734A (en) * | 2011-12-21 | 2014-10-08 | 明尼苏达大学董事会 | Cationic peptides and use of such peptides for inhibiting exotoxin production |
| CN104664130A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Polypeptide-containing aquatic microbial feed |
| CN104757320A (en) * | 2015-04-10 | 2015-07-08 | 烟台大乐饲料有限公司 | Functional compound feed special for greenhouse cultivation of litopenaeus vannamei |
| CN104770600A (en) * | 2015-04-10 | 2015-07-15 | 烟台大乐饲料有限公司 | Special penaeus orientalis functional biological feed in the thickness marking period |
| CN105995044A (en) * | 2016-06-13 | 2016-10-12 | 陕西中马环保科技有限公司 | Method for producing palm kernel meal protein feed through solid state fermentation |
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2017
- 2017-07-31 CN CN201710638731.2A patent/CN107467347A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102008006A (en) * | 2009-09-08 | 2011-04-13 | 中国农业科学院饲料研究所 | Preparation method of polypeptide for fermented feed with molecular weight of less than 15KDa |
| CN101828644A (en) * | 2010-05-11 | 2010-09-15 | 中山大学 | Feed for litopenaeus vannamei |
| CN104093734A (en) * | 2011-12-21 | 2014-10-08 | 明尼苏达大学董事会 | Cationic peptides and use of such peptides for inhibiting exotoxin production |
| CN104664130A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Polypeptide-containing aquatic microbial feed |
| CN104757320A (en) * | 2015-04-10 | 2015-07-08 | 烟台大乐饲料有限公司 | Functional compound feed special for greenhouse cultivation of litopenaeus vannamei |
| CN104770600A (en) * | 2015-04-10 | 2015-07-15 | 烟台大乐饲料有限公司 | Special penaeus orientalis functional biological feed in the thickness marking period |
| CN105995044A (en) * | 2016-06-13 | 2016-10-12 | 陕西中马环保科技有限公司 | Method for producing palm kernel meal protein feed through solid state fermentation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108514045A (en) * | 2018-03-12 | 2018-09-11 | 大连民族大学 | One kind having purification of water quality and the immunocompetent fancy carp microbial inoculum bait of raising and preparation method thereof |
| CN108514045B (en) * | 2018-03-12 | 2021-08-13 | 大连民族大学 | Koi microbial inoculum bait with water quality purification and immunocompetence improvement functions and preparation method thereof |
| CN110637928A (en) * | 2019-09-26 | 2020-01-03 | 海南农垦南金农场有限公司 | Method for preparing marine aquatic feed by taking jack fruit seeds as raw materials |
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Application publication date: 20171215 |